Tianwen Bai
Botuo Zheng
Jun Ling- MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China
To synthesize well-defined poly (α-amino acid)s (PAAs), ring opening polymerizations (ROP) of cyclic monomers of α-amino acid N-carboxyanhydrides (NCAs) and N-thiocarboxyanhydrides (NTAs) are most widely used. In this mini-review, we summarize the mechanism details of the monomer preparation and ROP. The present study used density functional theory calculations to reveal the mechanisms together with experimental phenomena in the past decades. Detailed discussion includes normal amine mechanism and the selectivity of the initiators bearing various nucleophilic groups.
Introduction
Poly(α-amino acid)s (PAAs) are promising biomaterials that have been employed in drug delivery, gene engineering, and self-assembly fields in recent years (Deming, 2016; Gangloff et al., 2016; Tao et al., 2018a; Birke et al., 2018). The synthetic process toward PAA includes ring opening polymerization of a cyclic monomer such as α-amino acid N-carboxyanhydrides (NCAs) and N-thiocarboxyanhydrides (NTAs), solid-phase synthesis, and biological methods (Mazo et al., 2020). NCAs were first prepared by Leuchs (Leuchs, 1906) in 1906, and NTAs were first reported by Aubert and Knott (1950). The ring opening polymerization (ROP) of cyclic monomers is still the most promising synthetic route toward PAAs. Due to the existence of multiple active sites located on NCA or in the NTA ring, including carbonyl groups at 2- and 5-positions for nucleophilic attack and acidic protons of 3-NH and 4-CH groups, various initiators have been carried out for polymerization including amines (Kricheldorf, 1987), trimethylsilyl (TMS) containing compounds, (Lu and Cheng, 2007, 2008; Yuan et al., 2016; Baumgartner et al., 2017; Yuan et al., 2018), salts (Szwarc, 1965; Wu et al., 2018), (activated) alcohols (Zhao et al., 2019; 2020), N-heterocyclic carbene (Guo and Zhang, 2009; Falivene et al., 2016; Falivene and Cavallo, 2017), transition metal catalysts (Deming, 1997), rare earth complexes (Ling et al., 2012; Peng et al., 2012; Tao et al., 2014), and so on.
The diversity of initiators leads to the complexity of ROP mechanisms, including normal amine mechanism (NAM), active monomer mechanism (AMM), carbene mechanism and so forth (Kricheldorf, 2006). NAM is considered responsible for living/controlled ROP, which follows chain propagation mechanism. Initiators in NAM usually bear at least one labile proton, as shown in Scheme 1. In mechanism investigation, NAM consists of three major steps, i.e., carbonyl addition, ring opening and decarboxylation. The rate-determining step of NAM is still under debate which is carbonyl addition or decarboxylation depending on monomers, initiators, and polymerization conditions. Differing from NAM, strong basic initiators prefer AMM, which is capable of ionizing cyclic monomers via deprotonation on 3-NH. As a result, AMM is only suitable for NCA and NTA but not N-substituted glycine NCA (NNCA) and the corresponding NTA (NNTA) monomers since they do not contain a proton on nitrogen atom.
SCHEME 1. NAM and AMM mechanisms for the ring opening polymerization of cyclic monomers toward poly(α-amino acid)s.
The experimental phenomena of AMM of NCA and NTA are similar to those of the general AMM of cyclic monomers, for instance, fast propagation rate, high monomer conversion in a short time, high molecular weight (MW), and broad dispersity of product, etc. Fast polymerization rate also makes AMM a practical method to prepare high-MW PAAs regardless of limited solubility. The details of AMM are not well-understood yet and lack information on reaction and overall kinetics analyses. AMM and NAM are sometimes found mixed, such as metal complex catalytic systems or initiation by mixtures of primary and tertiary amines.
For amine initiators, NAM and AMM have a boundary based on nucleophilicity and basicity. Primary amines follow NAM and tertiary amines follow AMM, but secondary amines depend on substituted groups. For linear substituted secondary amines such as propagation end in NNCA polymerization, good controllability and pseudo first kinetics are confirmed, but for some α-branched substituted secondary amines such as diisopropylamine obeys AMM. The nucleophilicity and basicity of amines are also influenced by interaction with other groups, especially in α,ω-telechelic initiators such as α,ω-alcohol/thiol amine (Tao et al., 2017; Tao et al., 2018b; Zhao et al., 2019; 2020).
Limited by our experimental tools, the molecular scale understanding of the mechanism is still lacking. Quantum chemistry tools such as density functional theory (DFT) can provide understanding at the molecular level, which could explain the essentials of NAM and AMM. In the present work, DFT investigation for monomer synthesis and NAM details are reviewed with some comments on unsolved mechanism problems.
Synthesis of NCA and NTA Monomers
The preparation of cyclic monomers is the first step toward PAAs. Hermann Leuchs synthesized NCAs from N-ethoxycarbonyl and N-methoxycarbonyl chloride via cyclization upon heating in vacuo. Such cyclization of N-alkoxycarbonyl halogenides toward NCAs is hence known as the Leuchs method (Leuchs, 1906; Leuchs and Manasse, 1907; Leuchs and Geiger, 1908), shown in Figure 1A. However, the Leuchs method cannot produce N-dinitrophenyl or N-acyl NCAs (Kricheldorf, 1987). The simplest and most widely-used method for NCA synthesis is a direct reaction between corresponding free amino acids with phosgene or its derivatives known as the Fuchs-Farthing route (Coleman and Farthing, 1950; Farthing and Reynolds, 1950). Benefiting from stability, NTAs can be carried out in an aqueous solution. Similar to the Leuchs route, the cyclization of N-ethoxythiocarbonyl amino acids with PBr3 or other ring-closing reagent was first carried out by Kricheldorf and Bösinger (Kricheldorf and Bösinger, 1976).
FIGURE 1. (A) Syntheses of NCA monomers via Leuchs and Fuchs-Farthing routes and NTA monomers via Kricheldorf route. (B) Two possible ring-closing pathways toward Ala-NCA and Ala-NTA following Leuchs route or Kricheldorf.
The critical compound in both the Leuchs and Kricheldorf routes is the N-alkyl(thio)carbonyl halogenides, which is too active to be caught in experimental work. After the ring closing process, some possible five-membered ring structures are proposed (Hirschmann et al., 1971; Vinick and Jung, 1982; Montalbetti and Falque, 2005). To investigate the synthetic details from the N-alkyl(thio)carbonyl halogenides to corresponding cyclic monomers, DFT calculations have employed model compound derived from alanine- (Ala-) and sarcosine- (Sar-) NCA or NTA (Bai et al., 2020). Two possible pathways are put forward as shown in Figure 1B.
For both NCA and NTA monomers, pathway 2 is preferred according to the comparison of the highest Gibbs free energy barriers in the whole process. In pathway 2, after the ring closing transition state (TS), the equivalent bromide anion is released. The bromide anion would further attack the methylene moiety neighboring oxygen with an SN2 substitution, which produces the final cyclic monomer via the corresponding TS. In the case of Ala-NCA, the rate-determining step is the SN2 step with a Gibbs free energy barrier of 22.0 kcal/mol under M06-2X/6-311++G(d,p) in the solvation model of dichloromethane. For Ala-NTA, the energy barrier of SN2 is 24.6 kcal/mol, which is a little higher than that of Ala-NCA.
A systemic study was carried out involving the effect of halides and N-substituents. As the starting reagents in the ring closing step, PBr3 is proposed to be a more active reagent than PCl3. DFT calculations confirm this based on the TSs along the pathways, with a benchmark analysis based on M06-2X, BHandH, and B3PW91/6-311++G(d,p). In TS of SN2 reaction, bromide is observed with longer interaction distance and more negative charge than chloride. For NNCA and NNTA, pathway 1 is forbidden due to the absence of hydrogen on nitrogen. Similar reaction pathways are found in the syntheses of alanine-NTA (Ala-NTA) and sarcosine-NTA (Sar-NTA), where Sar-NTA is confirmed with a lower energy barrier (18.5 kcal/mol) in the SN2 step compared with Ala-NTA (24.6 kcal/mol).
Racemization of NTA is of great importance due to the related chiral properties and secondary structures. The strong protonic acid is responsible for the racemization with SN2 TS, and the bulky electron cloud near α-C will suppress the racemization according to DFT calculations. For instance, leucine-NTA and isoleucine-NTA are harder to undergo racemization than Ala-NTA. The energy barrier (28.6 kcal/mol) of racemization is also noteworthy as it is higher than that of the synthesis of Ala-NTA (24.6 kcal/mol), which indicates that the racemization can be suppressed if shortening the reaction time.
Progress in Amine-Initiated NAM
In the last century, the search for the rate-determining step of NAM has become of great importance to research, since knowing it will enable chemists how to improve polymerization rate and controllability (Kricheldorf, 2006). Experimental results indicate that the rate-determining step can be one of carbonyl addition and decarboxylation, but still lacks sufficient evidence to conclude and may depend on initiators, monomers, and even polymerization conditions (Kricheldorf, 1987). For example, the polymerization is faster under an inert atmosphere, i.e., inert gas purged or in a vacuum, compared with that in sealed rubes for some NCAs (Habraken et al., 2011; Zou et al., 2013; Fan et al., 2014). Some NCAs are not (Habraken et al., 2011). It is also suspected that the dissolved CO2 decreases the reaction rate (Tao et al., 2014; Cao et al., 2017; Siefker et al., 2018). In some copolymerizations, it is the monomer species that determine the reaction rate (Shalitin and Katchalski, 1960). To provide a molecular scale understanding, DFT calculations do help. The first calculation work upon NAM was carried out by Ling and Huang (2010), taking L-Ala-NCA initiated by ethylamine as a model reaction. The NAM is divided into three main steps, i.e., carbonyl addition, ring opening, and decarboxylation. The reactivity of the 5-carboxyl group (5-CO) and 2-carboxyl group (2-CO) in the carbonyl addition step was examined, and it was confirmed that the carbonyl addition occurs at 5-CO. It was the first calculation evidence to support carbonyl addition step as rate-determining step.
After confirming the rate-determining step and three-step framework in the ROP of Ala-NCA initiated by ethylamine, it is of interest whether the NNCAs will follow the same mechanism. Compared with NCA, propagation end in NNCA ROP is a secondary amine rather than a primary amine due to the substitution on nitrogen, which may influence the whole NAM mechanism in both the carbonyl addition and decarboxylation steps and further change the rate-determining step. The DFT calculations of polymerizations of Ala-NCA as well as sarcosine-NCA (Sar-NCA) (Liu and Ling, 2015) are investigated with two initiators, i.e., primary amine and secondary amine with weak steric hindrance. The rate-determining steps in ROPs of NCA and NNCA initiated by either ethyl amine or dimethylamine, are the amine addition on the 5-carbonyl group rather than decarboxylation as before. These results are also benchmarked by Midix, Møller−Plesset perturbation theory, and coupled cluster theory, as shown in Figure 2A.
FIGURE 2. (A) The whole profiles of Gibbs free energy barriers in the ring opening polymerization of Ala-NCA initiated by dimethylamine and ethylamine with 3-dimensional models. Some hydrogen atoms are omitted for clarity. Thermodynamics and kinetic data, calculated by four basis set methods of energy barrier are listed. (B) The Gibbs free energy barriers in carbonyl addition step of six NNCAs in a vacuum and solution of benzonitrile. Transition states on the carbonyl addition step of NiPG-NCA and optimized geometry of an aggregated dimer with octyl side groups are illustrated with the distance between some hydrogen pairs in the unit of Å. Dash line is used as a guide. (C) The whole profiles of Gibbs free energies in ring opening polymerization of Ala-NCA initiated by MeNHTMS in benchmark. Reproduced with permission from corresponding references.
A small energy barrier difference was found in the carbonyl addition step between primary and secondary amine initiated cases. Based on the energy barrier, the reactivity ratios (r) of Ala-NCA and Sar-NCA are obtained with the product r1 × r2 = 1. Considering that ethylamine or dimethylamine are not only initiators but a simplified model of propagating end in ROP. The calculation results indicate that the random polymerization of NNCA and NCA is available especially for Ala-NCA and Sar-NCA. However, although the block copolymer of polypeptides-co-polypeptoids was obtained from both polypeptide and polypeptoid ends via sequential monomer feeding process (Birke et al., 2014; Heller et al., 2015), the synthesis of random poly(peptide-r-peptoid) has not yet been realized in experiments.
In the ROP of NNCA monomers, Sar-NCA has shown good reactivity in both experimental results and DFT calculations. Do NNCAs with large substituted groups have a similar polymerization mechanism to that of Sar-NCA? The side group effect in NNCA is of interest since the substituent group is related to the physical properties of polypeptoids such as hydrophilicity and crystallinity. In an experimental kinetic investigation (Fetsch et al., 2011) the ROP of NNCA follows pseudo first-order kinetics, and the apparent polymerization rate of NNCA is in the order of Sar >> EtGly > PrGly > nBuGly > iBuGly in benzonitrile. In the DFT calculation of the ROP of six NNCA monomers, the propagating chain ends rather than employing simple secondary amines to model real propagation (Bai and Ling, 2019). The rate-determining steps in all NNCA cases are still carbonyl addition in a three-step framework, but the energy barriers in the carbonyl addition step highly depend on the branched structure of substituents, as shown in Figure 2B. The steric hindrance causes strong repulsion of hydrogen pairs in carbonyl addition step, which leads to low reactivity of the β-branched amino acid such as NiPG-NNCA. In cases of linear and γ-C branched NNCAs, aggregation of side groups is observed in DFT calculations, which is considered responsible for low reaction rate instead of steric hindrance.
Other Labile Groups: NAM-TMS Mechanism
Can any group be the transfer moiety instead of proton in NAM? Trimethylsilyl (TMS) containing compounds, such as TMS-amines(Lu and Cheng, 2007, 2008; Baumgartner et al., 2017), have been found to successfully initiate living/controlled ROP of NCAs. DFT calculations have been carried out for the mechanism in ROP of Ala-NCA and Sar-NCA initiated by TMS amine with MeNHTMS as a model compound of initiator (Bai and Ling, 2017). In MeNHTMS, both proton and TMS groups can be transfer moiety in NAM. According to our DFT results, TMS transfer is thermodynamically favored compared with proton transfer in a benchmark of three functionals, as shown in Figure 2C. The mechanism is named as the “NAM-TMS mechanism”. In NAM-TMS, the CO2-TMS bearing compound is noteworthy as it has been observed to have great stability in both calculation and experimental work, which enlarges the energy barrier in the decarboxylation step, which becomes the rate-determining step. NAM-TMS is the first calculation evidence to support decarboxylation as a rate-determining step in NAM.
α,ω-Telechelic Amines
When α,ω-telechelic functionalized amines are employed as initiators, the end group of obtained PAA can be designed via adjusting interaction between two functionalized groups. Compared with NCA, NTA is more tolerable for water, hydroxyl, and thiol groups, which enables the convenient synthesis of α,ω-telechelic functionalized PAAs, such as ROP of NTA initiated α,ω-aminoalcohol(Tao et al., 2017) and cysteamine. (Tao et al., 2018b). In the case of some aminoalcohols, i.e., 2-amino-1-ethanol, 3-amino-1-propanol, and 4-aminomethylbenzyl alcohol, DFT calculations confirm that hydrogen bonding leads to the activation of hydroxyl group via 5- or 6-membered ring or π–π stacking in the initiation, and produces polypeptoid mixtures. By contrast, it is hard to activate thiol groups by amino groups, and the α,ω-cysteamine-initiated ROP selectively results in pure thiol-capped polypeptoids.
Summary and Perspectives
In amine-mediated ROP of NCA and NTA, integrated understanding of NAM has been established by DFT calculations including three-step framework, NAM-TMS mechanism, the effect of N-substitution in initiator or monomer ring, and the α,ω-functionalized initiator. Besides NAM, two problems have still not been solved in the ROP of NCA and NTA. The other mechanism, i.e., active monomer mechanism, is still not pieced in the whole picture puzzle. AMM contains more elemental reactions and more complicated kinetics than NAM. Another mechanism problem lies in the effect of chain structure, e.g., the acceleration induced by helix secondary structures (Baumgartner et al., 2017; Song et al., 2019) or macroinitiators.
Author Contributions
TB and BZ started the research and wrote the draft. JL supervised the project and revised the manuscript.
Funding
Financial support from the National Natural Science Foundation of China (21674091), the Key Research and Development Program of Zhejiang Province (2019C03014) and the National Key Research and Development Program from the Ministry of Science and Technology of China (No. 2016YFA0200301) is acknowledged.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: polypeptide, polypeptoid, quantum chemical calculation, mechanism and kinetic, polymer synthesis
Citation: Bai T, Zheng B and Ling J (2021) Density Functional Theory Studies on the Synthesis of Poly(α-Amino Acid)s Via the Amine-Mediated Ring Opening Polymerizations of N-Carboxyanhydrides and N-Thiocarboxyanhydrides. Front. Chem. 9:645949. doi: 10.3389/fchem.2021.645949
Received: 24 December 2020; Accepted: 17 February 2021;
Published: 29 March 2021.
Edited by:
Changkui Fu, The University of Queensland, AustraliaReviewed by:
Daniela Pappalardo, University of Sannio, ItalyGiovanni Talarico, University of Naples Federico II, Italy
Copyright © 2021 Bai, Zheng and Ling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jun Ling, lingjun@zju.edu.cn