Membrane Fouling in Algal Separation Processes: A Review of Influencing Factors and Mechanisms

Novoa, Andres Felipe; Vrouwenvelder, Johannes S.; Fortunato, Luca

doi:10.3389/fceng.2021.687422

REVIEW article

Front. Chem. Eng., 31 May 2021

Sec. Separation Processes

Volume 3 - 2021 | https://doi.org/10.3389/fceng.2021.687422

This article is part of the Research Topic Fouling in Membrane Filtration Systems View all 5 articles

Membrane Fouling in Algal Separation Processes: A Review of Influencing Factors and Mechanisms

$\nAndres Felipe Novoa$ Andres Felipe Novoa

Johannes S. Vrouwenvelder

Luca Fortunato^*

Division of Biological and Environmental Science and Engineering (BESE), Water Desalination and Reuse Center (WDRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia

The use of algal biotechnologies in the production of biofuels, food, and valuable products has gained momentum in recent years, owing to its distinctive rapid growth and compatibility to be coupled to wastewater treatment in membrane photobioreactors. However, membrane fouling is considered a main drawback that offsets the benefits of algal applications by heavily impacting the operation cost. Several fouling control strategies have been proposed, addressing aspects related to characteristics in the feed water and membranes, operational conditions, and biomass properties. However, the lack of understanding of the mechanisms behind algal biofouling and control challenges the development of cost-effective strategies needed for the long-term operation of membrane photobioreactors. This paper reviews the progress on algal membrane fouling and control strategies. Herein, we summarize information in the composition and characteristics of algal foulants, namely algal organic matter, cells, and transparent exopolymer particles; and review their dynamic responses to modifications in the feedwater, membrane surface, hydrodynamics, and cleaning methods. This review comparatively analyzes (i) efficiency in fouling control or mitigation, (ii) advantages and drawbacks, (iii) technological performance, and (iv) challenges and knowledge gaps. Ultimately, the article provides a primary reference of algal biofouling in membrane-based applications.

GRAPHICAL ABSTRACT

Graphical Abstract. Schematic illustration of the interactions among algal cells, algal organic matter (AOM), and transparent exopolymer particles (TEP) in algal membrane fouling in separation processes. The external organic matter (EOM) is the AOM that results from the algal metabolic activity, while the internal organic matter (IOM) refers to the AOM released due to cell rupture. dEOM and bEOM refer, respectively, to the dissolved and bound fractions.

Introduction

Microalgae comprise a diverse group of prokaryotic and eukaryotic organisms capable of using CO₂ and nutrients, such as nitrogen (N) and phosphorous (P), to produce organic matter and oxygen via photosynthesis (Roostaei and Zhang, 2017). Owing to its distinctive rapid growth and the versatility to be cultivated in a wide range of environments, the large-scale production of microalgae as a source of biofuels, food, and valuable products has gained momentum in recent years (Roostaei and Zhang, 2017; Tua et al., 2021). More recently, algal growth has been coupled to water treatment, employing wastewater as a feed containing nutrients necessary, such as carbon, nitrogen, and phosphorous (Kanchanatip et al., 2016; Luo et al., 2017; Sun et al., 2021). In addition to this, microalgae can be cultivated, utilizing CO₂ from unconventional sources, such as power plants and cement manufacturing facilities, thus reducing carbon dioxide emissions (Senatore et al., 2021). Thereby, combining water treatment and algal production offsets the costs of both processes by optimizing the use of energy, water, and resources (Xu et al., 2015; Liao et al., 2018; Luo et al., 2019; Sun et al., 2021).

The potential benefits of algal biotechnologies are affected by their high production cost (Tua et al., 2021). Using wastewater might be a competitive source to replace energy-intensive commercial fertilizers, yet algal harvesting and dewatering remain as major challenges (Roostaei and Zhang, 2017; Alshahri et al., 2021a). In fact, it is estimated that 90% of the total cost of algal biomass production may come from harvesting and dewatering (Zhang et al., 2014), whereas the latter contributes up to 60–70% of the cost (Chen et al., 2012; Liao et al., 2018; Zhang and Fu, 2018; Zhang Y. et al., 2019). The traditional solid-liquid separation technologies for microalgae, such as coagulation/flocculation, centrifugation, gravity sedimentation, and dissolved air flotation, impact the capital expenses of algal production as they demand large amounts of energy, chemicals, or footprint (Bilad et al., 2014; Zhang and Fu, 2018). Compared with conventional microalgal separation processes, membrane technologies proved to be a cost and energy-efficient alternative for removing a wide range of particles and organisms, lowering the solid-liquid separation costs and reducing the chemicals consumption (Mu et al., 2014; Xu et al., 2015; Gonçalves et al., 2017; Luo et al., 2017; Bamba et al., 2021; Senatore et al., 2021; Zhao et al., 2021a; Zheng et al., 2021). However, membrane fouling and the consequent decline in the permeate flux remain a major challenge to membrane applications on the cultivation, harvesting, and dewatering of algal biomass, as well as to downstream separation processes for recovery of value-added products (Adhikari and Pellegrino, 2015; Shi et al., 2019; Sun et al., 2021; Zhao et al., 2021a). Therefore, an increasing number of publications addressing algal fouling and membrane algal fouling have been published in recent years (Figure 1).

FIGURE 1

Figure 1. Number of publications reported in the Scopus database on the topics of algal fouling.

A broad number of studies have highlighted the influence of several factors affecting algal biofouling and separation performance (Le-Clech et al., 2005; Fletcher et al., 2011b; Krzeminski et al., 2017; Luo et al., 2017; Bagheri and Mirbagheri, 2018; Fortunato et al., 2020; Jiang et al., 2020; Zhou et al., 2020; Sun et al., 2021). These aspects include membrane characteristics, such as pore size and membrane materials; characteristics, such as algal species and cell size (Kanchanatip et al., 2016; Elcik and Cakmakci, 2017; Keyvan Hosseini et al., 2020); metabolic products, including fractions of proteins and carbohydrates (Kanchanatip et al., 2016; Shekhar et al., 2017; Wu et al., 2018; Luo et al., 2019; Zhang Z. et al., 2020; Zhou et al., 2020); and operating conditions, such as the hydraulic retention time (HRT) and solids retention time (SRT) (Low et al., 2016; Novoa et al., 2020).

Considering the high fouling propensity in algal membrane separation processes, fouling control strategies are crucial to ensure their long-term operation (Eliseus et al., 2017; Zhang and Fu, 2018; Fortunato et al., 2020; Hua et al., 2020). Different strategies, including physical and chemical membrane cleaning, have been employed to increase the membrane life span and reduce the impact of fouling on the process (Zhao et al., 2021a). However, the increase in the consumption of energy and chemicals is a common drawback in fouling controls (Le-Clech et al., 2005; Fletcher et al., 2011b; Krzeminski et al., 2017; Luo et al., 2017; Bagheri and Mirbagheri, 2018). In this review, an updated summary of the contributing factors and the underlaying mechanisms to algal fouling is presented. It is found out that a comprehensive understanding of the dynamic interactions between foulants and the membrane during operation is critically important in the development and implementation of fouling control strategies.

Impact of Algal products on Fouling Mechanisms

Membrane fouling is governed by the interactions between mixed liquor and the membrane, whereas the convective forces toward the membrane trigger the deposition of foulants on the surface. Over time, fouling results in a loss of water permeability through the membrane, thereby increasing transmembrane pressure (TMP) required to drive the process (Fletcher et al., 2011b; Bilad et al., 2014; Krzeminski et al., 2017). Fouling is commonly classified into different categories based on the foulants characteristics (i), permeability recovery (ii), or fouling mechanism (iii).

According to the origin of foulants, the fouling can be classified as organic, inorganic, or biofouling. Inorganic fouling, or scaling, results from the chemical precipitation of inorganic particles onto the membrane surface or into the membrane pores. Organic fouling includes extracellular polymeric substances (EPS), proteins, carbohydrates, and humic substances present in the algal organic matter (AOM). In algal membrane photobioreactors (MPBRs), the term biofouling mainly refers to the accumulation of algal cells, organic matter, and transparent exopolymer particles (TEP) (Fletcher et al., 2011b; Bilad et al., 2014; Wang et al., 2014; Liao et al., 2018; Zhang Z. et al., 2020). It is worth noting that, in algae processes, there is no clear distinction between organic fouling and biofouling, as, most of the time, they occur simultaneously.

Based on permeability recovery, fouling can be considered reversible, irreversible, or permanent. Reversible fouling usually refers to the fouling fraction that can be easily removed by physical cleaning such as backwash or relaxation. Irreversible fouling, on the other hand, is defined as the fraction that can be removed only by intensive chemical cleaning. Finally, permanent fouling is defined as the fraction that cannot be recovered by any cleansing agent (Fletcher et al., 2011b; Bilad et al., 2014; Wang et al., 2014) (Table 1).

TABLE 1

Table 1. Definitions in fouling classification according to the permeability recovery, fouling mechanism, and cleaning efficiency.

A variety of membrane-foulant and foulant-foulant interactions result in different fouling mechanisms, namely, pore-blocking, pore-narrowing, and cake layer formation. The sorption of particles and colloidal substances with a size smaller than the membrane pores narrow the membrane channels, whereas the sorption of particles sized similar to the membrane pores causes pore-blocking (Fletcher et al., 2011b; Bilad et al., 2014; Wang et al., 2014; Marbelia et al., 2016; Elcik and Cakmakci, 2017; Krzeminski et al., 2017; Keyvan Hosseini et al., 2020; Novoa et al., 2020; Zhou et al., 2020). In contrast, particles and solutes with a size larger than that of the membrane pores, are rejected and accumulated on the membrane surface due to size exclusion, forming a cake layer. The cake layer is a complex matrix composed of foulants like cells, debris, inorganic colloidal particles, and AOM, which provides additional hydraulic resistance to the filtration and reduces the water permeability (Liao et al., 2018; Zhang and Fu, 2018; Zhang M. et al., 2019). The cake layer is often reversible, depending on its adhesive properties (Bin et al., 2017; Fortunato et al., 2020; Zhou et al., 2020). In fact, Bin et al. (2017) described higher reversible cake layer resistance when algal cells or debris is accumulated on the vicinity of the membrane surface, in divergence with cell debris and IOM that showed a greater tendency to irreversible fouling.

On the other side, it is worth mentioning the eventual positive impact of the cake layer: A compact and more resistant cake layer acts as a secondary filtration layer, protecting the membrane surface from small organics molecules responsible for irreversible fouling (Low et al., 2016; Fortunato et al., 2020; Novoa et al., 2020). Javadi et al. (2014) found out that an increase in the cake layer resistance, induced by a compact and non-porous structure, improved the permeate quality (Javadi et al., 2014). This finding is in agreement with the reported positive effect of biofouling on MBR systems, whereby the accumulation of microbial EPS and SMP was found to further improve the rejection of organic micropollutants that would otherwise permeate UF/MF membrane pores (Deng et al., 2016; BouNehme Sawaya and Harb, 2021).

Stages of Algal Fouling

Algal biofouling results from the deposition and accumulation of AOM, algal cells, and TEP present in the mixed liquid solution (Fletcher et al., 2011b; Bilad et al., 2014; Wang et al., 2014; Liao et al., 2018; Zhou et al., 2020). The fouling in algal membrane processes is described as a three-stages process (Babel and Takizawa, 2010; Low et al., 2016). Biofouling is, hence, firstly built upon AOM coatings on the membrane surface during a stage named “conditioning fouling,” characterized by strong membrane-foulant interactions that result in higher rates of pore-blocking and narrowing that depend on the interfacial electrochemistry and steric forces, and result in the formation of a gel layer and short TMP jumps. Thereupon, microfouling with colonies of unicellular algae is facilitated by the adsorbed and conditioned foulants; this second fouling stage, or slow fouling, results in a cake layer composed of multicellular algal complexes that lead to a linear TMP rise as the cake becomes thicker (Fletcher et al., 2011b; Bagheri and Mirbagheri, 2018; Liao et al., 2018). Finally, the third fouling stage is characterized by a sharp exponential rise in fouling rates, which is caused by the progressively heterogeneous distribution of foulants on the membrane surface and cake layer; the permeability in the fouled areas is hampered, and the flux is redirected toward less fouled areas wherein the critical flux is exceeded (Babel and Takizawa, 2010; Fletcher et al., 2011b; Bagheri and Mirbagheri, 2018; Novoa et al., 2020).

The first stage (conditioning fouling) is strongly influenced by the interactions between algal foulants and the membrane surface; these interactions are widely described by the extended Derjaguin–Landau–Verwey–Overbeek model (XDLVO) in terms of interfacial free energy variations (ΔG_adh) before and after adhesion. The total free energy of adhesion is considered as the sum of acid–base (AB), van der Waals (LW), and electrostatic (EL) interactions (Blumreisinger et al., 1983; Ozkan and Berberoglu, 2013). LW originates from asymmetrical distributions of electrons, and it is usually attractive; on the other hand, EL interactions refer to the Coulombic interactions between foulants and a membrane; and, finally, the AB forces represent the attractive interactions between similarly hydrophobic species and the repulsive interactions between hydrophobic surfaces and hydrophilic species (Ozkan and Berberoglu, 2013). The magnitude of the total AB, LW, and EL interactions controls the reversibility of a foulant's adhesion (Blumreisinger et al., 1983).

As algal fouling develops, the membrane–cell interactions are weakened, and the fouling development is mostly influenced by the cell–cell and cell–AOM interactions in the cake layer (Fletcher et al., 2011b; Wu et al., 2013; Bilad et al., 2014; Kanchanatip et al., 2016; Marbelia et al., 2016; Elcik and Cakmakci, 2017; Ting et al., 2017; Liao et al., 2018; Zhang and Fu, 2018; Zhang M. et al., 2019). In fact, Zhou et al. (2020), Novoa et al. (2020), and Low et al. (2016) report that, in the continuous operation mode, upon the cake layer formation, the XDLVO forces are strongly weakened by convective and hydrodynamic forces. Similarly, Marbelia et al. found out that, in hydrophilic negatively charged membranes, the correlation between flux and XDLVO interactions decrease after 120 min of operation, as the deposition of foulants neutralizes the charge of the membrane surface, decreasing its ability to repel AOM and algal cells (Marbelia et al., 2016).

Despite the commonalities reported in the fouling development process of biological sludge and microalgae, the differences in foulants composition and their interactions with the membrane led to different fouling mechanisms (Fletcher et al., 2011b; Wu et al., 2013; Bilad et al., 2014; Kanchanatip et al., 2016; Marbelia et al., 2016; Elcik and Cakmakci, 2017; Ting et al., 2017; Liao et al., 2018; Zhang and Fu, 2018; Zhang M. et al., 2019). In algal processes, the cells present large sizes and are completely retained by UF and MF membranes, and, thus, the cake layer constitutes a significantly larger fraction of total fouling when compared with other processes, such as MBRs. Conversely, a gel layer and pore adsorption have been found to exert a larger contribution to membrane fouling in bacterial sludge processes (Deng et al., 2016).

Algal Foulants

Algal Cells

The sizes, shapes, and surface properties strictly depend on the algae species (Liao et al., 2018; Zhang and Fu, 2018; Zhou et al., 2020). The algal sizes vary around 3–30 μm, resulting bigger than the pore diameters of MF and UF membranes, and, thus, it becomes the primary constituent of the cake layer due to size exclusion (Zhang and Fu, 2018; Sun et al., 2021). A higher cake porosity is commonly observed in species with bigger cells (Bilad et al., 2013; Marbelia et al., 2016; Zhang and Fu, 2018). Shekhar et al. (2017) investigated the relation between particle size and cake resistance in algal suspensions of Chlorella vulgaris and Chlamydomonas reinhardtii; the latter displayed a thicker cake layer and higher resistance to water permeation. Analogous results were reported by Marbelia et al. (2016), in studying the filterability of different algal species, using polyacrylonitrile (PAN) membranes; large particles, near to spherical shape and with a numeric narrow size distribution, led to a higher cake porosity and better filterability properties. Similarly, Bilad et al. found an enhancement in filterability when using cells with spherical shape and rigid cell walls, such as Chlorella vulgaris, in magnetically induced membrane vibrating (MMV) systems (Bilad et al., 2013); and Zhou et al. (2020) studied the size-dependent adhesive properties of Chlorella vulgaris and Scenedesmus obliquus that induced different filter abilities. To this extent, different results highlight that large, spherical, and rigid cells present the best filterability characteristics (Bilad et al., 2013; Marbelia et al., 2016; Liao et al., 2018; Zhang and Fu, 2018; Zhou et al., 2020).

Both cake layer compressibility and membrane performance are strongly affected by the cell walls rigidity (Zhang and Fu, 2018). Hereafter, algal species with rigid cell walls, as is the case of Chlorella and Phaeodactylum, lead to the formation of a rigid cake layer that exhibits low compressibility and, thereby, a constant porosity and better filterability over time (Baerdemaeker et al., 2013; Marbelia et al., 2016; Zhang and Fu, 2018; Zhang M. et al., 2019). In contrast, species with compressible cell walls, such as Nannochloropsis, are associated with sharper TMP increases and a significant reduction in permeability (Babel and Takizawa, 2010).

Another factor that contributes to membrane fouling is the biomass concentration, which increases the viscosity of the bulk medium and hinders the air scouring effect across the membrane surface (Fletcher et al., 2011b; Bagheri and Mirbagheri, 2018). Kanchanatip et al. reported a direct correlation between biomass concentration and flux decline; cell solids were found to contribute as primary foulants, leading to 63 and 86% water flux decrease when operating, respectively, at 10 and 40 g/L (Kanchanatip et al., 2016). Similarly, Bamba et al. (2021) reported a permeate flux declined from 441 to 73 LMH when the feed concentration of Chlorella vulgaris increased from 0.25 to 1 g/L.

It is worth noting that algal cell concentration cannot be considered a stand-alone parameter to describe the fouling propensity. Indeed, the cells are in suspension with large amounts of AOM (Javadi et al., 2014; Bamba et al., 2021). Thereby, indicators targeting the interactions between algal cells and AOM in solution, such as the shear rate efficiency and the particle size distribution (PSD), presented a better correlation with the fouling rates (Fletcher et al., 2011b; Javadi et al., 2014; Kanchanatip et al., 2016; Low et al., 2016).

The PSD elucidates the size properties of the particles that result from the dynamic equilibrium between formation, transformation, and breakage of algal aggregates (Barbusiński and Kościelniak, 1995). Smaller particles lead to a more compact and non-porous structure, exhibiting higher cake specific resistance when compared with larger particles (Marbelia et al., 2014; Bourcier et al., 2016). Bourcier et al. studied the impact of PSD in the specific cake resistance and compressibility; a broad and heterogeneous PSD was found to build a cake constituted by uneven layers with different particle sizes, where the smaller ones were prone to move between layers as the filtration pressure increased (Bourcier et al., 2016). In fact, the uneven deposition of particles with wide-size distribution at different locations in the cake layer negatively impacts the permeability, causing the flux to redistribute toward less-fouled areas and leading to a rapid rise in TMP (Fletcher et al., 2011b; Bourcier et al., 2016; Novoa et al., 2020).

Algal Organic Matter (AOM)

The soluble organic matter released by algae represents a main operational challenge in algal membrane processes since it strongly affects the membrane fouling potential (Luo et al., 2019). These compounds vary in chemical compositions and molecular weights, depending on several factors, including the algal characteristics, the nutrients availability, and the growth phase (Huang et al., 2012; Zhang et al., 2016; Zhuang et al., 2016). Common terms and classifications of AOM are presented in Figure 2.

FIGURE 2

Figure 2. Algal organic matter (AOM) classification according to sources and characteristics. The AOM is considered the main contributor to irreversible fouling in algal membrane separation processes. The AOM can form gel-like structures and irreversibly attach to the membrane pores and surfaces.

Based on its molecular weight, the AOM can be classified into four categories: (1) high molecular weight (MW) biopolymers (>20,000 Da); (2) humic-like substances (500–20,000 Da); (3) medium molecular weight components, such as building blocks (350–500 Da); and (4) low-MW neutrals (<350 Da) (Zhang W. et al., 2013). High-MW biopolymers, due to size exclusion, tend to accumulate on the membrane surface, contributing to cake layer resistance (Zhang et al., 2016; Bin et al., 2017). Similarly, in a characterization of the organic compounds present in a MPBR, Luo et al. (2019) described the relation between rejection of organics and their molecular weight: Biopolymers were largely rejected (84%), followed by building blocks (<42%) and low molecular weight compounds (42–63%). Due to their size, low molecular weight compounds were reported to be more prone to penetrate into the membrane pores and cause pore-blocking and irreversible fouling (Morineau-Thomas et al., 2002; Kanchanatip et al., 2016; Luo et al., 2019). Hence, the cake layer built upon high-MW biopolymers might result beneficial, as it acts as a secondary and reversible sieving layer that protects the membrane surface from irreversible low-MW foulants.

Proteins and carbohydrates present in the AOM exhibit hydrophilic and hydrophobic fractions that vary according to the algal species and growth phase (Zhang and Fu, 2018). A larger hydrophilic fraction is reported in carbohydrates, characterized for forming stronger intermolecular bridges through hydrogen bonding (Zhang W. et al., 2013; Zhang X. et al., 2013). These interactions increase the adsorption rates and the share of irreversible fouling in the system (Zhang W. et al., 2013; Bin et al., 2017). In contrast, proteins are associated with amino acid chains with hydrophobic side groups that exhibit weak interactions with hydrophilic membranes (Henderson et al., 2008), leading to a loose and reversible fouling (Zhang W. et al., 2013; Zhang et al., 2016; Xu et al., 2018; Zhou et al., 2020). Thereby, proteins have been found to exhibit stronger hydrophobic characteristics compared with carbohydrates, thus with a greater tendency to act as charge neutralizers while forming “gel-like” substances (Henderson et al., 2008; Keyvan Hosseini et al., 2020).

AOM that results from the algal metabolic activity is termed “external organic matter” (EOM), whereas the internal organic matter (IOM) refers to the AOM released due to cell rupture. The relative fractions of EOM and IOM, as well as size distribution and hydrophobicity, vary depending on the algal growth phase; in logarithmic growth, AOM is mainly composed of EOM as the cells have good integrity and death or lysis does not occur (Bin et al., 2017; Zhou et al., 2020). However, as the biomass reaches a stationary phase, the cell aging and lysis trigger the release of IOM into the solution, increasing drastically the total AOM in solution (Zhuang et al., 2016; Bin et al., 2017; Liu et al., 2017; Zhang and Fu, 2018). Carbohydrates are reported to be the main component of EOM, whereas the IOM is seemingly richer in proteins (Zhang and Fu, 2018). Contradictory results on the impact of carbohydrates and proteins suggest that their effect on membrane fouling is species dependent and relies on the algal growth conditions. Bin et al. (2017) and Xu et al. (2021) both report the stronger impact of IOM on flux decline (Zhang et al., 2016; Bin et al., 2017), while Low et al. (2016) and Novoa et al. (2020) report a stronger impact of carbohydrates on membrane fouling. However, all the studies highlighted the stronger tendency of EOM to irreversibly deposit onto the membrane surface (Luo et al., 2019). To this extent, strategies targeting the prevention of cell breakage are desired to keep the cell integrity required for the recovery of value-added products (Adhikari and Pellegrino, 2015; Shi et al., 2019).

The fraction of EOM that is dissolved in the algal suspension is named “dissolved EOM” (dEOM), while the fraction bound to the cell surface is regarded as bound EOM (bEOM). Both dEOM and bEOM are mainly composed of organics with sizes in the high and low fractions (>100 and <1 kDa), although dEOM presents a larger share of low-MW organics and greater hydrophilicity (Zhang X. et al., 2013). Qu et al. (2012) reported that dEOM accounted for 80% of the total EOM of M. aureginosa. Thereafter, it remains thoroughly possible to develop operational strategies devised to shift the hydrophilicity of dEOM and to prevent its detachment from algal cells, thus controlling the impact of EOM on irreversible fouling.

By studying the growth of the biofouling layer in the algal bioreactor, Luo et al. (2019) found out that the deposition of foulants across the fouling layers is size dependent. The small-MW organics exhibited high affinity to attach and build upon the membrane surface, while the biopolymers displayed a weak interaction with the membrane surface and, thus, were present at low concentration in the bottom fouling layer (<10%). Zhou et al. (2020) confirmed these findings by reporting a preferential deposition of algal cells and debris on the upper cake layer, while larger amounts of organic compounds were contained in the chemically desorbing layer. Furthermore, the lower part of the cake layer of Chlorella vulgaris was found to present a larger amount of proteins, while the carbohydrates were mainly distributed on the upper part of the layer; this is in contrast with another study, reporting larger amounts of carbohydrates on the bottom layer of Arthrospira Platensis (Keyvan Hosseini et al., 2020) and, thereby, support the hypothesis that the distribution of foulants across the cake layer varies among species.

Similar to algal cells, the study of AOM as a fouling precursor needs to be addressed, starting from the inter-foulant interaction with cells and debris (Novoa et al., 2020; Zhang Y. et al., 2020). Therefore, Bin et al. (2017) evaluated the impact of algal cells, debris, EOM, and IOM on the membrane fouling; the relative flux reductions were 10, 27, and 16% for debris + IOM, cells + EOM, and debris + IOM + EOM, respectively. The authors found out also that the combined algal foulants led to less-severe adsorptive fouling in comparison with individual foulants, supporting the previous hypothesis of a competitive adsorption of AOM, cells, and debris, and indicating that larger inter-foulant aggregates exert a positive effect on the long-term filterability (Bin et al., 2017; Zhou et al., 2020). Thereby, the cake layer density is determined by the cell–cell interaction, cell–membrane entanglement, and hydrogen bonding interaction between AOM and algal cells (Novoa et al., 2020; Zhang Z. et al., 2020).

Transparent Exopolymer Particles (TEPs)

A particular case of AOM, termed “transparent exopolymer particles” (TEPs), attracted increasing research due to its significant contribution to membrane fouling. TEPs are characterized by presenting high viscosity, reaching values up to 0.21 Pa•s (Logan et al., 1995). In fact, TEPs are reported to be about 2–4 orders of magnitude stickier and more flexible than phytoplankton or mineral particles, conferring a gel-like structure that significantly increases the probability of attachment upon collision (Bilad et al., 2014; Liao et al., 2018; Zhang Z. et al., 2020). Therefore, TEPs act as an adsorption and condensation nuclei of dissolved fouling precursors, forming aggregates that severely hamper the membrane permeability (Zhang and Fu, 2018; Zhang Z. et al., 2020). Furthermore, upon attachment onto the membrane surface, TEPs act as a scaffold between organisms and the membrane, building a 3D-stable structure that is easily deformed upon the increase in pressure and serves as an ideal surface for the attachment and growth of algae (Zhang Z. et al., 2020).

TEPs and their precursors are generally sticky and highly hydrophilic and comprise mainly of acidic glycoproteins and carbohydrates. TEPs present sizes that range from 0.05 to 200 μm (Passow et al., 2001; Berman and Holenberg, 2005; Zhang Z. et al., 2020), and, according to their size, can be classified as colloidal (0.05–0.4 μm) and particulate (>0.4 μm), although one form converts into the other given certain conditions. Comparably to AOM, the TEPs can also be classified as bound or free TEP (_bTEP and _fTEP), based on its presence in attachment with algal cells or free in solution (Zhang Z. et al., 2020). Zhang Z. et al. (2020) investigated the effect of _bTEP and _fTEP on UF membrane fouling; _bTEP led to a faster flux decline at the beginning of the filtration. However, over time, _bTEP reached a maximum plateau level, and _fTEP became the main fouling contributor; _bTEP was found to precede larger reversible fouling than _fTEP (10.8–15.6% higher), whereas _fTEP was the main factor leading to irreversible fouling (37.8 vs. 32.9%). Thereupon, the cake layer formed by _bTEP was more easily removed by backwash than that formed by _fTEP, and a larger fraction of proteins was found in the latter. Based on the above-described studies, understanding the fraction of TEP in fouling will likely play a more significant role in the development of fouling control strategies in membrane applications in algal technologies.

Combined Effect in Membrane Fouling

The cake layer permeability is conditioned by the forces acting upon the attachment of algal particles on the membrane (Babel and Takizawa, 2010; Marbelia et al., 2016). In principle, in absence of AOM, the cake resistance increases linearly with the number of cells deposited (Babel and Takizawa, 2010). However, microalgae release AOM and, thus, a dynamic structure are built upon the linkages between algal cells and the AOM, as reported in the literature (Morineau-Thomas et al., 2002; Babel and Takizawa, 2010; Kanchanatip et al., 2016; Zhou et al., 2020). EOM, IOM, and TEP fill void spaces between algal cells and drastically increase the cake resistance (Graphical Abstract). The interactions between algal cells and AOM also determine the size and stability of the algal flocs. To this extent, larger aggregates with homogeneous size distribution are preferred to secure long-term filterability (Bin et al., 2017; Zhou et al., 2020). It is worth noting that the membrane fouling in algal separation processes is governed by several factors (Figure 3).

FIGURE 3

Figure 3. Summary of fouling contributing factors in algal membrane separation processes. The membrane fouling is influenced by the operating conditions, the membrane, the algal species, and AOMs.

Impact of Membranes on Algal Fouling

The membrane proprieties and configuration influence their surface properties and define the dominating fouling mechanism and overall fouling severity (Figure 3). The membrane materials directly impact properties, such as morphology (roughness, pore size, and distribution), hydrophilicity/hydrophobicity and electric charge (Liao et al., 2018; Zhang and Fu, 2018; Mat Nawi et al., 2020). On the other hand, membrane configuration also impacts the hydrodynamics during permeation and cleaning; for example, hollow-fiber membranes are characterized by higher packing density, allowing higher permeate productivity, albeit increasing the chances of intermembrane clogging and lowering the scouring efficiency during cleaning (Fletcher et al., 2011b; Bagheri and Mirbagheri, 2018; Liao et al., 2018).

Pore Size

An appropriate membrane pore size is required for an optimal operation, as it determines the retention of algal biomass during harvesting and the dominating fouling mechanism (Liao et al., 2018; Zhang and Fu, 2018; Mat Nawi et al., 2020). The molecular weight distribution of foulants relative to the membrane pore size regulates the fouling propensity, and, thus, the impact of membrane pore size on the fouling development has been widely investigated (Fletcher et al., 2011b; Bilad et al., 2014; Wang et al., 2014; Marbelia et al., 2016; Elcik and Cakmakci, 2017; Krzeminski et al., 2017; Liao et al., 2018; Zhang and Fu, 2018; Zhang Y. et al., 2019). Smaller pores reject a wider range of foulants, increasing the cake layer resistance that can be removed by cleaning techniques such as backwash. Nevertheless, small pores exert higher resistance to water permeation, increasing the energy consumption and creating a trade-off between permeability and fouling control (Fletcher et al., 2011b; Bilad et al., 2014; Wang et al., 2014; Marbelia et al., 2016; Elcik and Cakmakci, 2017; Krzeminski et al., 2017; Liao et al., 2018; Zhang and Fu, 2018; Zhang Y. et al., 2019).

In spite of presenting higher permeate flux, larger pore sizes are more prone to irreversible fouling (i.e., pore-blocking or adsorption) (Low et al., 2016; Liao et al., 2018; Novoa et al., 2020). This was observed by Elcik and Cakmakci (2017) in a comparison between ultrafiltration (UF) and microfiltration (MF) membranes in cross-flow filtration; larger pore sizes in MF exhibited faster flux decline and presented a higher fouling severity than that of UF membranes. Accordingly, Rickman et al. (2012) compared the filterability properties of UF and MF membranes, using pre-filtered suspensions of Chlamydomonas; MF displayed a higher irreversible fouling propensity, presumably due to the internal pore plugging of submicron particles, while in the UF prevailed the cake layer resistance. In contrast, Zhao et al. (2017) and Bilad et al. (2018) reported the opposite trend: Larger pore sizes resulted in milder fouling and higher permeability, leading to a lower TMP increase. Zhao et al. linked this positive effect to the different drag forces prevailing, as larger pore sizes lowered the hydraulic resistance and reduced the deposition of foulants onto the membrane (Zhao et al., 2017). Contradictory results support the idea that the impact of membrane pore size on fouling development relies on foulant-membrane interactions that are species dependent (Zhang Y. et al., 2019).

Materials and Hydrophobicity/Hydrophilicity

Membrane materials used in algal biotechnologies can be classified into organic and inorganic (Zhang Y. et al., 2019). Polymeric organic materials used in membranes include polyvinylidene fluoride (PVDF) (Marbelia et al., 2018), polyacrylonitrile (PAN) (Marbelia et al., 2016), polyethersulfone (PES) (Yogarathinam et al., 2018), cellulose acetate (CA), polyethylene terephthalate (PET) (Kim et al., 2020; Kusumocahyo et al., 2020; Wu and Sancaktar, 2020), polyvinyl alcohol (PVA), regenerated cellulose (RC), polysulfone (PS) (Ma et al., 2020; Zhao et al., 2021a,b), polyamide (PA), and cellulose ester (CE) (Zhang M. et al., 2019). Inorganic materials, such as ZrO₂-TiO₂, are commonly used in ceramic membranes (Low et al., 2016; Hua et al., 2020). PVDF membranes are widespread in microalgal filtration due to their high thermal and chemical resistance; however, their hydrophobic nature results in poor electrostatic interaction with water molecules and stronger interactions with foulant species (Bilad et al., 2018; Marbelia et al., 2018). On the other hand, hydrophilic membranes exhibit a higher anti-fouling capacity with respect to hydrophobic membranes, owing to a reduced adsorption of hydrophobic protein-like substances and dissolved macromolecular compounds (Liao et al., 2018; Marbelia et al., 2018; Zhang Y. et al., 2019). However, the low mechanical resistance of hydrophilic membranes hinders their widespread application (Bilad et al., 2014; Liao et al., 2018; Mat Nawi et al., 2020). Different materials exhibit differences in surface charge and hydrophobicity/hydrophilicity; thereby, surface modification techniques, such as coatings and grafting, have been employed to develop robust and fouling-resistant membranes (Marbelia et al., 2018).

The use of hydrophilic materials, such as nanofiber membranes, has been increased over the last years due to their efficiency in microalgae harvesting, although their mechanical strength still represents a bottleneck (Liao et al., 2018; Marbelia et al., 2018; Zhang Y. et al., 2019; Mat Nawi et al., 2020). Accordingly, Mat Nawi et al. (2020) investigated the applicability of electrospinning mechanically enhanced nylon 6.6 nanofiber membranes (NFM) in algal filtration. The crossing and overlapping of NFM fibers reduced thickness, porosity, and mean pore size by 18, 4, and 40%, respectively, the effect, created by the fusion and melting of fibers, increased the mechanical resistance of the membrane by more than 2-fold (221%). Additionally, the surface modification reduced surface roughness and water contact angle by 63 and 28%, respectively, increasing the overall membrane hydrophilicity (Mat Nawi et al., 2020). Similarly, Bilad et al. (2018) compared the performance of nylon 6.6 nanofiber membrane (NFM) with a PVDF phase inverted membrane (PVDF-PIM) for filtration of Chlorella vulgaris; Nylon 6.6 NFM was found to have higher permeability (1,018 vs. 493 L/m².h) and better fouling resistance than PVDF-PIM. This was attributed to the lower contact angle (0.25 vs. 76), the larger pore size (0.12 vs. 0.09 μm), and a higher surface porosity (25.82 vs. 18.84%), compared with PVDF-PIM. Moreover, it is worth mentioning that lowering the surface roughness is targeted in membrane design to control the fouling deposition and, more importantly, to lower the water contact angle and enhance the membrane hydrophilicity (Liao et al., 2018; Mat Nawi et al., 2020).

Recent studies have explored the use of PET-based membranes due to their commercial availability, low price, and stability (Kusumocahyo et al., 2020; Wu and Sancaktar, 2020). Kim et al. (2020) used an anthracene (ANT)-attached polyethylene glycol (PEG) surface-modification agent to create a hydration layer by coating the surface with brush and arch forms. The resulting hydrolyzed membrane exhibited an improved antifouling effect and microalgal productivity compared with the unmodified one. Analogously, Marbelia et al. (2018) used PVP cross-linking modification and sulfonation to enhance the hydrophilicity and antifouling properties of PVDF membranes; the water permeability and fouling resistance increased by PVP modification in four different algal species (Danuliella, Picochlorum, Scenedesmus, and Diacronema), indicating the efficiency of hydrolyzed membranes as a fouling control strategy in algal separation. Recently, PET self-forming dynamic membranes have been proposed in an innovative photobioreactor, allowing harvesting biomass at rates of 41 g/m² 2021 (Senatore et al., 2021).

Electric Surface Charge

In addition to hydrophilicity, surface charge and electrostatic interactions play significant roles in the membrane fouling potential. Negatively charged hydrophilic membranes are reported to enhance the electrostatic repulsion with negatively charged AOM and algal cells, inducing an antifouling effect on the membrane surface (Liao et al., 2018; Marbelia et al., 2018). Accordingly, Marbelia et al. (2016) evaluated the filterability of different microalgae species when using hydrolyzed negatively charged PAN-based membranes. For most species, negatively charged membranes mitigated the fouling development during the first 120 min of filtration. However, thereupon, the antifouling effect faded, indicating that the electrostatic repulsion forces become less significant over time, as hydrodynamic interactions become the main parameter, controlling fouling development. Similarly, applying direct currents (dc) to conducting materials allowed to successfully remove organics and mitigate membrane fouling in electrochemically reactive ceramic membranes made of TiO₂ (Hua et al., 2020); the antifouling effect was tested at different dc (−1.25 to +2.5 mA.cm⁻²), proving to be efficient at mitigating membrane fouling and maintaining higher critical flux when using positive currents. The effect was ascribed to radical electrostatic repulsion and formation of microbubbles on the membrane surface.

Impact of Operating Conditions

Environmental Conditions (pH, Temperature, and Light)

Environmental conditions, such as pH, temperature, and light, significantly impact the fouling severity by influencing the algal growth, the AOM, and the cell surfaces. The pH influences the degree of protonation/deprotonation and, thus, the surface charge in algal foulants and the electrostatic repulsive/attractive interactions with the membrane; lower pH values create positive surface charges by increasing the concentration of amine groups, whereas, at high pH, these groups are deprotonated, inducing a net negative surface charge (Zhou et al., 2014; Zhang and Fu, 2018). Comparatively, under acidic conditions, the repulsive interactions between TEP and UF membranes significantly decreased (41.7%) in comparison with neutral and alkaline conditions, facilitating the adhesion of free TEPs onto the membrane and, thereby, increasing the irreversible membrane fouling (Zhang Z. et al., 2020).

The pH-dependent surface charges exert a direct effect on the floc properties and coagulation efficiency (Zhou et al., 2014; Xu et al., 2021). As reported by Zhou et al. (2014), the low pH intensifies the interactions between the EOM and the membrane surface, thus increasing the membrane fouling. Analogously, Xu et al. (2021) evaluated the impact of pH on coagulation; the results highlighted the effect of the pH on the isoelectric point (pH_iep) of different coagulants, impacting the floc size and formation rate and, subsequently, the cake layer filterability. Acidic conditions favored the isoelectric point of a titanium xerogel coagulant, enabling the aggregation of algae and organic matter upon dosing; alkaline conditions, on the other hand, proved to be inadequate for achieving charge neutrality, and, thereby, the aggregation was lower. Finally, it is worth noting that increases in the pH associated with algal photosynthesis increased the formation of mineral foulants and, thereby, the inorganic fouling resulting from the precipitation of phosphorus, calcium, and iron (Hu et al., 2015).

Light and temperature affect the enzymatic activity of microalgae and the diffusivity of organic matter (Liao et al., 2018; González-Camejo et al., 2019). The increase in temperature reduces the water viscosity and increases the membrane permeability; thereafter, the drag forces toward the membrane are weakened, and the deposition of microalgal cells is reduced (Zhao et al., 2015; Liao et al., 2018). Furthermore, the temperature influences the enzymatic activity, impacting the AOM production (Liao et al., 2018). In fact, Zhao et al. (2015) observed a decrease in the secretion of EOM by increasing the temperature from 15 to 30°C. On the other hand, the light influences also microalgal productivity and death and lysis of the algal cells. Furthermore, variations in the light/dark cycles can induce a cell lysis and, thereby, increase the IOM concentration in solution (González-Camejo et al., 2019). A photoinhibition effect was found at light intensities beyond 1,800 μmol.m⁻²s⁻¹ (González-Camejo et al., 2019).

Based on the notions described above, it is reasonable to secure environmental conditions that assure cell integrity while favoring charge neutrality and aggregation. Further to this, adjusting light cycles/intensity and pH results challenging in the long-term operation, as it directly impacts the algal biomass quality.

Nutrients, Hydraulic Retention Time (HRT), and Solids Retention Time (SRT)

In terms of nutrients, the food to the microorganism ratio (F/M) relates to the substrate provided per unit of volume of algae (Metcalf and Eddy, 2003). At low F/M ratios, algal flocs are characterized by compact arrangements that are characterized by high stability (Barbusiński and Kościelniak, 1995; Medina and Neis, 2007). While, at high F/M ratios, the flocs exhibit a high propensity to break up and, thereby, resulting in a heterogenous size distribution of floc aggregates (Barbusiński and Kościelniak, 1995; Medina and Neis, 2007; Ashadullah et al., 2021). This effect was investigated by Novoa et al. (2020), reporting fewer stable aggregates and a broader particle size distribution when higher nutrients were supplied at shorter HRT; the resulting cake layer formed on the membrane surface consisted of heterogeneous particles, and, thus, the void spaces between large particles were filled by the smaller ones, lowering the cake porosity and increasing its resistance to permeation. The results are in agreement with the reported effect of F/M in conventional MBRs (Liao et al., 2002, Wu et al., 2013); for example, higher F/M ratios were reported by Ashadullah et al. (2021) and Wu et al. (2013) to induce a larger surplus of the substrate that triggered the excretion of EPS, while, on the other hand, low F/M ratios decreased the bacterial growth rates, increasing endogenous metabolism and cell lysis (Metcalf and Eddy, 2003; Fletcher et al., 2011b; Zhang M. et al., 2020).

The F/M ratio is directly affected by parameters such as the HRT and SRT, which refer, respectively, to the residency time of feed solution and the microalgal biomass in the reactor. The HRT directly impacts the loading rate of nutrients into the reactor, whereas an upsurge in nutrients is observed at lower HRT. The SRT impacts the retention time of the algal biomass in the reactor, and, thereby, lower SRT limits the contact time between algae and nutrients, lowering the uptake of nutrients needed for metabolic growth. On the other hand, higher biomass retention times at longer SRTs might result in greater biomass concentrations, as the nutrients are efficiently removed from the feed at a constant HRT (Metcalf and Eddy, 2003; Fletcher et al., 2011b; Ashadullah et al., 2021; Zhang M. et al., 2020).

The impacts of HRT and SRT directly affect the metabolic growth and, thereby, the fouling mechanisms and overall severity. Novoa et al. (2020) assessed the fouling behavior of Chlorella vulgaris cultivated in an MPBR at HRTs of 12, 24, and 36 h; higher nutrient loading at HRT_12h impacted the PSD and the AOM production, leading to the formation of a dense cake layer on the membrane surface. On the other hand, a longer HRT (HRT_24h and HRT_36h) resulted in a narrower PSD, leading to a more moderate fouling and stable flux. Similarly, Low et al. (2016) reported an increase in the average particle size from 8.5 to 13.1 μm, when the HRT was reduced from 72 to 6.5 h. The decrease in HRT was followed by the increase of the biopolymer concentrations and by the formation of large and heterogenous aggregates in the reactor, leading to the development of a dense cake layer with higher hydraulic resistance. Moreover, the longer HRT_72h enhanced the hydrolysis of the organic molecules into low MW compounds, responsible for a milder and yet more irreversible fouling, compared with a high MW compound (i.e., biopolymers).

The impact of the N/P ratio and nutrient depletion conditions on the filtration performance has been evaluated by several authors (Huang et al., 2012; Zevin et al., 2015; Zhang M. et al., 2020). Zhang Z. et al. (2020) evaluated the effects of the HRT and N/P ratio on fouling propensity. At the same N/P ratio, shorter HRTs led to severe fouling. Conversely, at equal HRTs, lower N/P ratios (3.9:1) showed smaller floc sizes and faster fouling rates compared with higher ratios (9.7:1). Huang et al. (2012) reported an increase of the hydrophilic fraction at a lower N/P ratio (1:10) in discrepancy with higher ratios (1:2 and 1:1). Similarly, Zevin et al. (2015) analyzed the SMP in two PBRs, cultured with Synechocystis sp., operated under normal (PBR_P+) and limited phosphate (PRB_P0) conditions. PBR_P+led to greater biomass production while lowering the SMP production per unit of biomass, compared with PBR_P0. The latter exhibited a larger concentration of carbohydrates due to the phosphate limitation.

Based on the results reported in the literature, it might be desired to target high N/P ratios and longer HRTs. However, the extreme nutrient limitation might result in the breakup of large biopolymers into small-MW organics and into the endogenic release of IOM, resulting from a cell lysis, increasing the fouling potential. Conversely, short HRTs and, thereby, high-nutrient loading rates result in faster metabolic growth, whereas the EOM fraction prevails over irreversible IOM. In summary, the balance between the nutrient-loading rates and the residence time of algal biomass determines the concentration of the IOM/EOM in the membrane reactor.

Flux and Hydrodynamic Conditions

The hydrodynamic conditions in algal membrane processes are governed by several factors, including flux, cross-flow velocity and air scouring. These parameters impact the fouling behavior, as they exert great influence on the algal cell deposition rates, cell integrity, and permeate drag forces. A conventional approach to mitigate the fouling in the system consists of reducing the permeate drag force by applying mild flux and increasing the shear (Zhang and Fu, 2018; Bamba et al., 2021).

When operating at constant flux, the deposition of fouling on the membrane surface leads to a significant increase of the transmembrane pressure (TMP) required to achieve constant flux production (Liao et al., 2018; Zhang and Fu, 2018). An alternative strategy consists of operating under subcritical flux conditions to decrease the convective forces that drag the foulants toward the membrane. However, although reducing the permeate flow rate can be used to mitigate the fouling developed on the membrane, it significantly impacts the capital cost by requiring larger membrane areas (Fletcher et al., 2011b). Wu et al. (2018), compared the filtration performance of Chlorella vulgaris under sub and supercritical flux conditions (22 and 44 LMH, respectively); as expected, the subcritical flux displayed a more stable filtration performance and lower irreversible fouling (7 vs. 10%) compared with supercritical flux conditions. The latter displayed larger resistance (61% higher), thereby exhibiting a higher overall contribution to fouling (9.2 vs. 2%). Analogously, similar results are reported by Kanchanatip et al. when characterizing the flux decline of Arthrospira maxima; the results confirm that higher permeate velocity increases the particle's deposition rates on the membrane surface (Kanchanatip et al., 2016).

Another parameter that affects the filtration performance is the fluid cross-flow velocity (CFV). The CFV is directly linked to the turbulence and shear force and, thereby, allows to decrease the accumulation of microalgae on the membrane, achieving higher filtration flux (Elcik and Cakmakci, 2017; Liao et al., 2018; Bamba et al., 2021). Elcik and Cakmakci (2017) evaluated the microalgal harvesting at different CFVs. At a TMP of a 1.5 bar, cross-flow velocities of 0.35 and 1.57 m/s led to fluxes of 65 and 195 LMH, respectively, demonstrating that increasing the CFV is associated with enhanced filtration flux, as it was thereafter confirmed by Bamba et al. (2021) in ceramic microfiltration of Chlorella vulgaris and Tisochrysis lutea. Similarly, Ozkan and Berberoglu reported that, at a flow rate of 1 ml min⁻¹, the net force acting on a cell adhered over a substratum, decreased from 1.9 ×10⁻¹¹ to 3.3 ×10⁻¹² N as the effective cell diameter interacting with the membrane decreased from 10.4 to 4.3 μm. At a velocity of 1.5 mm s⁻¹, the kinetic energy of Chlorella vulgaris increased to counter the attractive energy between cells and a substratum, thus preventing the attachment on the surface (Ozkan and Berberoglu, 2013). It is worth to mention that there is a critical CFV that negatively impacts the cell integrity and the transmission of AOM across the membrane after being overpassed (Wicaksana et al., 2012; Ozkan and Berberoglu, 2013). This effect was observed for CFV values above 0.17 m/s (Wicaksana et al., 2012).

Membrane Cleaning

As membrane fouling exacerbates, the need of frequent membrane cleaning to keep constant flux becomes a major challenge as it reduces the efficiency of algae harvesting and permeate flux and reduces the lifetime of the membranes (Zhao et al., 2018). Thereby, choosing an adequate membrane cleaning mechanism is of vital importance in terms of energy consumption, permeate production, and overall process performance (Zhao et al., 2018; Fortunato et al., 2020).

Cleaning strategies, namely backwash, relaxation, and air scouring, are commonly employed in the operation of a submerged reactor, displaying different effects on fouling control. The relaxation temporary stops the filtration, thus allowing the release of foulants from the cake layer into the solution via back transport (Mat Nawi et al., 2020); backwash, on the other hand, consists of reversing the permeate flow, allowing the partial detachment of the fouling layer and the removal of the particles attached to the membrane surface, thereby reducing the compaction of the gel polarization layer and alleviating the fouling caused by pore blockage and cake formation (Fletcher et al., 2011b; Wang et al., 2014; Bagheri and Mirbagheri, 2018); finally, air scouring employs gas bubbles that move across the membrane surface, resulting in localized shear stress conditions that scour the foulants attached on the outer fouling layer and promote a turbulent flow regime that facilitates their back transport into the solution (Mat Nawi et al., 2020).

Fortunato et al. (2020) investigated the impact of membrane cleaning techniques by monitoring the algal biomass morphology developed on the membrane surface under continuous operation. The use of relaxation led to a thicker and more porous algal cake that, in turn, displayed better filterability properties and lower energy consumption compared with backwash (Figure 4). Membrane cleaning techniques present, however, some drawbacks; long relaxation periods reduce the filtration time, hampering the permeate productivity (Fortunato et al., 2020). The impact on productivity is even more severe in backwash, as large backwash flow rates are reportedly required to remove strongly attached LMW organics, thus diminishing significantly the permeate produced and exerting extra energy inputs, analogous to the one caused by the large energy demands of air scouring (Fortunato et al., 2020; Mat Nawi et al., 2020). Therefore, a porous and permeable cake layer is reportedly preferred in operation, owing its effect of sieving irreversible foulants. The effect of air scouring beyond a shear threshold limits the cake thickness and, in turn, increases the severity of irreversible fouling caused by LMW-organics that directly interact with the membrane (Fortunato et al., 2020; Mat Nawi et al., 2020).

FIGURE 4

Figure 4. Algae biomass morphology developed on the membrane surface under different fouling cleaning strategies in submerged algae membrane photobioreactor. The biomass morphology was monitored non-destructively in-situ with optical coherence tomography (OCT), adapted from Fortunato et al. (2020).

Recent developments have aimed to optimize the shear effect created by air scouring systems. Eliseus et al. (2017) designed a customized tilted membrane panel that maximized the local shear and lift forces created by air bubbles during air scouring for filtering Euglena sp.; the permeability was improved by 20–30% as the tilting angle was increased up to 20°, maximizing the contact between air bubbles and the membrane surface (Eliseus et al., 2017). Accordingly, Mat Nawi et al. (2020) tilted the panel of nanofiber membranes to 20° to improve the fouling control effect of membrane cleaning cycles; increasing the aeration rates enhanced the permeability up to 379 LMH.

Emerging cleaning strategies attempt to reduce the area wherein shear stress is applied (Bamba et al., 2021; Zhao et al., 2021b). This effect has proved to be efficient at controlling fouling, while securing cell integrity and thus limiting the release of irreversible foulants. For example, dynamic filtration systems apply a relative motion, either by vibration or rotation, in the membrane-foulant interface within the range of 0–0.5 mm of distance, and, thereby, the algal cells in the feed are protected from shear-induced damage, while the shear is efficiently applied on the cake layer and concentration polarization layers (Zhang and Fu, 2018; Jiang et al., 2020).

Developments and Challenges of Algal Fouling Control

The study of the dynamic interactions involved at the membrane-foulant interface led to the developments of several algal fouling control strategies (Figure 5, Table 2). Most of the strategies were initially adopted from other membrane filtration processes (i.e., MBR for wastewater treatment). However, there are fundamental differences between bacterial and algal processes. For example, in the activated sludge MBRs, the main focus is the removal of pollutants and the recovery of clean permeate. In contrast, in algal membrane-based processes, one of the main objectives is the recovery of valuable algal biomass (Deng et al., 2016; Liao et al., 2018). Therefore, traditional fouling control techniques differed essentially by the targeted final products, although significant similarities have emerged in recent years, as algal production has been coupled to wastewater treatment (Liao et al., 2018; Liu et al., 2018).

FIGURE 5

Figure 5. Summary of fouling control approaches in algal membrane separation processes.

TABLE 2

Table 2. Summary of recent findings on algal fouling precursors and their effect on fouling development.

The first group of algal fouling control strategies attempted to modify the filterability properties of algal foulants present in the feed solution, using pretreatment methods and controls in operation. Feed pretreatment relies on the use of coagulants and adsorbents to promote the formation of cells and AOM aggregates that, in turn, are easily removed from the membrane surface and show better filterability properties upon attachment (Huang et al., 2019; Xing et al., 2019; Zhang Y. et al., 2020; Xu et al., 2021). Alternatively, oxidants are increasingly used in pretreatment to reduce and mineralize the concentration of the smaller organics fraction responsible for irreversible fouling (Liu et al., 2018; Lee et al., 2019, 2021; Wan et al., 2019; Ren et al., 2020). Despite their positive impacts on the fouling mitigation, primary challenges arose from concerns related to the effect of the chemical agents on the algal biomass, their release into the environment, and the high energy and capital expenses demanded by these techniques (Discart et al., 2015; Alshahri et al., 2021b).

Membrane operation is often controlled to target specific flux and cross-flow velocity (CFV), whereby the attractive forces between foulants and membrane are reduced by increasing the shear rates in the reactor (Liao et al., 2018; Zhang and Fu, 2018; Bamba et al., 2021). Moreover, operating parameters, such as the HRT and SRT, might be adjusted to control the rates of EOM/IOM generation and, thereby, the characteristics of the aggregates and prevailing fouling mechanism (Low et al., 2016; Novoa et al., 2020).

More recently, upon improved knowledge of membrane fouling, a second generation of control strategies has focused on the disruption of foulant-foulant and foulant-membrane interactions by mechanical means in dynamic filtration systems and by modifications of membrane materials and surface geometry (Ladner et al., 2010; Zhang and Fu, 2018; Zhang Y. et al., 2019). Membrane modifications have been recently applied by coating the surface with polymers and inorganic nanoparticles, aiming to control the overall electrostatic repulsion/attraction and hydrophobic/hydrophilic interactions in the membrane-foulant interface (Liu et al., 2019; Soleimani et al., 2021; Zhao et al., 2021a; Zheng et al., 2021). Hence, recent efforts in surface coating with hydrophilic monomers, surface grafting with hydrophilic polymer bushes, and nanomaterial incorporation have been developed to improve the filtration performance (Hu et al., 2015; Liao et al., 2018; Liu et al., 2019; Ma et al., 2020; Mat Nawi et al., 2020). In addition, the incorporation of TiO₂, Fe₂O₃, zeolites, silica, and silver has been found to increase the hydrophilicity of the membrane, thereby enhancing the permeate flux (Hu et al., 2015). However, the significantly higher energy demand and the increase in the cost exerted by these modifications represent a bottleneck for these approaches, and, therefore, developing cost-efficient antifouling membrane materials and geometries with high hydrophilicity and mechanical resistance remains a subject of study (Ladner et al., 2010; Zhang and Fu, 2018; Zhang Y. et al., 2019; Zhao et al., 2021b). To this extent, it is necessary to integrate these aspects into the developments of fouling control technology, while comparative techno-economic and environmental assessments of fouling control strategies should be evaluated to decrease the cost and the environmental impact of the process.

Future Perspectives and Conclusions

This paper focuses on a comprehensive literature review on precursors and mechanisms of algal fouling. It was found out that the properties affecting the interactions in the membrane-feed interface directly impact the fouling mechanism and its overall contribution to the membrane filtration performance. The characteristics of algal cells, AOM, and TEP, as well as their roles in membrane fouling, were reviewed. Algal cells are the main fouling contributor, leading to high reversible resistance that is easily removed by cleaning means; a cake layer, made of large and rigid cells with spherical shapes, is reported to present the best filterability properties, while these aspects are strongly determined by the algal species. On the other hand, AOM is the main contributor of irreversible fouling; it is mainly composed of hydrophobic proteins that form gel-like structures and hydrophilic carbohydrates that irreversibly attach to the membrane pores and surfaces. Controlling operating conditions to minimize the release of IOM and small-MW organics and promoting the aggregation of AOM and algal cells into larger particles are considered key factors to mitigate membrane fouling and to reach longer cycles of biomass harvesting. Moreover, understanding the underlying mechanisms responsible for the release of TEP into the solution remains a priority in fouling control.

Over recent years, different fouling control techniques have been developed in algal processes based on a growing understanding of the underlying interactions between the foulants and membrane surfaces. These strategies include changes in the operating conditions, pretreating the feed via coagulation, adsorption, or oxidation, cleaning the membranes by physical or mechanical means, and modifying the membrane surfaces by coatings or blends with polymers and nanoparticles. Despite the impact on membrane operation, very limited studies exist on real-time fouling characterization, and current assessments rely on indirect fouling indicators, such as TMP and concentration of foulants. Thereby, novel fouling characterization and assessment techniques to further understand the interaction between algal foulants and a membrane surface demand further study.

In spite of recent progress in control strategies, fouling remains the main challenge for membrane-based algal technologies. Understanding algal fouling still presents major knowledge gaps; the dynamics across the cake layer impacting the cake porosity and overall filterability demand further research. Further understanding of these dynamics might drive the development of novel in-situ membrane fouling control systems, whereby localized shear rates or cleaning minimizes the cell breakage in solution while reducing the overall resistance of gel and a cake layer. Likewise, environmental considerations should be integrated in the future fouling control developments, aiming to optimize their economic performance of algal separation processes and minimize environmental impacts.

Author Contributions

AN and LF: conceptualization and investigation. AN: writing—original draft preparation. LF, AN, and JV: writing—review and editing. All authors have read and agreed to the published version of the manuscript.

Funding

The research reported in this paper was supported by funding from King Abdullah University of Science and Technology (KAUST).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

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