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CORRECTION article

Front. Cell Dev. Biol., 21 June 2024
Sec. Stem Cell Research
Volume 12 - 2024 | https://doi.org/10.3389/fcell.2024.1407164
This article is part of the Research Topic Understanding Human Biology with iPSC Derived Cell Types in the Era of CRISPR Technology View all 6 articles

Corrigendum: ACE2 knockout hinders SARS-CoV-2 propagation in iPS cell-derived airway and alveolar epithelial cells

This article is a correction to:

  1. ACE2 knockout hinders SARS-CoV-2 propagation in iPS cell-derived airway and alveolar epithelial cells

    1. Read original article
Ryo Niwa,Ryo Niwa1,2Kouji Sakai, Kouji Sakai3,4*Mandy Siu Yu Lung&#x;Mandy Siu Yu Lung1†Tomoko MatsumotoTomoko Matsumoto1Ryuta Mikawa,Ryuta Mikawa2,5Shotaro Maehana,Shotaro Maehana6,7Masato SuzukiMasato Suzuki8Yuki Yamamoto&#x;Yuki Yamamoto2†Thomas L. Maurissen&#x;Thomas L. Maurissen1†Ai HirabayashiAi Hirabayashi9Takeshi Noda,Takeshi Noda9,10Makoto Kubo,Makoto Kubo6,7Shimpei Gotoh, Shimpei Gotoh2,5*Knut Woltjen Knut Woltjen1*
  • 1Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
  • 2Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • 3Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan
  • 4Management Department of Biosafety, Laboratory Animal and Pathogen Bank, National Institute of Infectious Diseases, Tokyo, Japan
  • 5Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
  • 6Department of Microbiology, Kitasato University School of Allied Health Sciences, Kanagawa, Japan
  • 7Regenerative Medicine and Cell Design Research Facility, Kitasato University School of Allied Health Sciences, Kanagawa, Japan
  • 8Antimicrobial Resistance Research Center, National Institute of Infectious Diseases (NIID), Tokyo, Japan
  • 9Laboratory of Ultrastructural Virology, Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan
  • 10Laboratory of Ultrastructural Virology, Graduate School of Biostudies, Kyoto University, Kyoto, Japan

A Corrigendum on
ACE2 knockout hinders SARS-CoV-2 propagation in iPS cell-derived airway and alveolar epithelial cells

by Niwa R, Sakai K, Lung MSY, Matsumoto T, Mikawa R, Maehana S, Suzuki M, Yamamoto Y, Maurissen TL, Hirabayashi A, Noda T, Kubo M, Gotoh S and Woltjen K (2023). Front. Cell Dev. Biol. 11:1290876. doi: 10.3389/fcell.2023.1290876

In the published article, there was an error. The Results section regarding the reported proportion of DNA sequence outcomes for one gRNA contained a mistake. While the data in the associated figure was correct, an error in the main text was identified during a subsequent review of our data.

A correction has been made to Results, ACE2 knockout by MMEJ-based guide RNA design, Paragraph 3. This sentence previously stated:

“We evaluated gRNAs ACE2x138, ACE2x484, and ACE2x1371 for KO activity in the B2-3 lung reporter iPS cell line (Gotoh et al., 2014). Gene editing outcomes were confirmed by Sanger sequencing and TIDE analysis (Figure 1C). While ACE2x138 did not demonstrate any detectable indels with this assay, ACE2x484 and ACE2x1371 both showed indel formation (42% and 60.1%, respectively). In the ACE2x484 polyclonal population, 15% of indel alleles were represented by the predicted del7 mutation, while for ACE2x1371 the predicted del8 mutation was represented only 3.9% of the total population. For both gRNAs, ins1 mutations were observed in the TIDE data (14.7% and 39.9%, respectively). The ACE2x484 gRNA had the highest MENTHU score in exon five and is also supported by being in the top four in VBC score top two in BioScore (Supplementary Table S4).”

The corrected sentence appears below:

“We evaluated gRNAs ACE2x138, ACE2x484, and ACE2x1371 for KO activity in the B2-3 lung reporter iPS cell line (Gotoh et al., 2014). Gene editing outcomes were confirmed by Sanger sequencing and TIDE analysis (Figure 1C). While ACE2x138 did not demonstrate any detectable indels with this assay, ACE2x484 and ACE2x1371 both showed indel formation (42% and 49.3%, respectively). In the ACE2x484 polyclonal population, 15% of indel alleles were represented by the predicted del7 mutation, while for ACE2x1371 the predicted del8 mutation was represented only 0.8% out of the total population. For both gRNAs, ins1 mutations were observed in the TIDE data (13.1% and 9.2%, respectively). The ACE2x484 gRNA had the highest MENTHU score in exon 5. It also ranked fourth when evaluated using the VBC score and was the second best based on the BioScore out of 13 gRNA on exon 5 (Supplementary Table S4)”.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: iPS cells, CRISPR-Cas9, gene editing, gene knockout, ACE2, SARS-CoV-2

Citation: Niwa R, Sakai K, Lung MSY, Matsumoto T, Mikawa R, Maehana S, Suzuki M, Yamamoto Y, Maurissen TL, Hirabayashi A, Noda T, Kubo M, Gotoh S and Woltjen K (2024) Corrigendum: ACE2 knockout hinders SARS-CoV-2 propagation in iPS cell-derived airway and alveolar epithelial cells. Front. Cell Dev. Biol. 12:1407164. doi: 10.3389/fcell.2024.1407164

Received: 26 March 2024; Accepted: 20 May 2024;
Published: 21 June 2024.

Edited and reviewed by:

Joel W. Blanchard, Icahn School of Medicine at Mount Sinai, United States

Copyright © 2024 Niwa, Sakai, Lung, Matsumoto, Mikawa, Maehana, Suzuki, Yamamoto, Maurissen, Hirabayashi, Noda, Kubo, Gotoh and Woltjen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Kouji Sakai, ksakai@nih.go.jp; Shimpei Gotoh, gotoh.shimpei.5m@kyoto-u.ac.jp; Knut Woltjen, woltjen@cira.kyoto-u.ac.jp

†Present address: Yuki Yamamoto, HiLung Inc, Kyoto, Japan
Thomas L. Maurissen, Roche Pharma Research and Early Development, Immunology, Infectious Diseases and Ophthalmology, Roche Innovation Center Basel, F Hoffmann-La Roche Ltd., Basel, Switzerland
Mandy Siu Yu Lung, aceRNA Technologies, Innovation Hub Kyoto, Kyoto, Japan

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.