In the published article, there were errors in Figure 6, Supplementary Figure S4 and the legend for Supplementary Figure S3. The representative image of the “GFP+Myc-Hsp90” group in Figure 6B and the image of the “GFP-NudC 0 h” in Supplementary Figure S4B were misused due to carelessness. In the figure legend for Supplementary Figure S3D, the description of the scale bar should be 50 μm, but not 100 μm. The corrected Figure 6, Supplementary Figure S4 and figure legend for Supplementary Figure S3 appear below.
FIGURE 6
The authors apologize for these errors and state that these mistakes do not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2023.1163790/full#supplementary-material
Supplementary Figure S3Downregulation of filamin A suppresses cell migration in AGS cells. Cells treated with filamin A RNAi or not were subjected to the following analyses. (A) Western blotting analysis showed the expression of filamin (A). GAPDH, a loading control. (B,C) Scratch wound assays revealed cell migration at the different time points. The scratch closure was recorded with DIC microscopy. Dashed lines indicated the approximate line of wound edges. The distance between the two edge lines was measured by ImageJ software. Scale bar, 100 μm. (D,E) Transwell migration assays were performed to detect cell motility. Cells that migrated to the undersides of the filters were stained with 0.2% crystal violet and monitored with DIC microscopy. The number of migrated cells per transwell was counted. Scale bar, 50 μm. (F–H) The migration tracks of individual cells were traced by Imaris 9.1.2 software. Euclidean distance and migration velocity were analyzed with Imaris 9.1.2 software. Quantitative data are presented as the means ± SD (at least three independent experiments). n, the sample size. *P < 0.05; **P < 0.01. Student’s t-test.
Supplementary Figure S4Overexpression of NudC-L279P suppresses cell migration in AGS Cells. AGS cells stably overexpressing GFP, GFP-NudC, or GFP-NudC-L279P were subjected to following analyses. (A) Western blotting analysis of the expression levels of the indicated proteins. GAPDH, a loading control. (B,C) Scratch wound assays revealed cell migration at the different time points. The scratch closure was recorded with fluorescence microscopy. Dashed lines defined the approximate line of wound edges. The distance between the two edge lines was measured by ImageJ software. (D,E) Transwell migration assays were carried out to detect cell motility. Cells that migrated to the undersides of the filters were stained with 0.2% crystal violet and monitored with DIC microscopy. The number of migrated cells per transwell was counted. (F–H) The migration tracks of individual cells were traced by Imaris 9.1.2 software. Euclidean distance and migration velocity were analyzed with Imaris 9.1.2 software. Scale bars, 100 μm. Quantitative data are presented as the means ± SD (at least three independent experiments). n, the sample size. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant (P > 0.05). Student’s t-test.
Summary
Keywords
cell migration, filamin A, Hsp90, NudC-L279P, protein stability
Citation
Liu M, Xu Z, Zhang C, Yang C, Feng J, Lu Y, Zhang W, Chen W, Xu X, Sun X, Yang M, Liu W, Zhou T and Yang Y (2023) Corrigendum: NudC L279P mutation destabilizes filamin a by inhibiting the Hsp90 chaperoning pathway and suppresses cell migration. Front. Cell Dev. Biol. 11:1163790. doi: 10.3389/fcell.2023.1163790
Received
11 February 2023
Accepted
20 March 2023
Published
28 March 2023
Volume
11 - 2023
Edited and reviewed by
Ben Goult, University of Kent, United Kingdom
Updates
Copyright
© 2023 Liu, Xu, Zhang, Yang, Feng, Lu, Zhang, Chen, Xu, Sun, Yang, Liu, Zhou and Yang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Tianhua Zhou, tzhou@zju.edu.cn; Yuehong Yang, yhyang@zju.edu.cn
†These authors have contributed equally to this work
This article was submitted to Cell Adhesion and Migration, a section of the journal Frontiers in Cell and Developmental Biology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.