Abstract
Efferocytosis is a process by which phagocytes remove dead or dying cells. It is considered anti-inflammatory, as the removal process reduces potential inflammatory molecules originating from dead cells and results in the reprogramming of macrophages to an anti-inflammatory state. However, engulfment of infected dead cells, deregulated phagocytosis and perturbed digestion of apoptotic bodies induce inflammatory signalling pathways during efferocytosis. The affected inflammatory signalling molecules and the mechanism of activation are largely unknown. I discuss how the choice of dead cell cargo, the type of ingestion, and the digestion efficiency can influence phagocyte programming in the context of disease. I also present the latest findings, highlight knowledge gaps, and propose selected experimental approaches to fill them.
Introduction
The adage ‘Nothing is certain but death’ (modified from Benjamin Franklin) is valid for all cells in our body. From their formation to their last signalling events, cells balance pro-survival and pro-death signalling triggered by external and internal cues. Pathogens, DNA damage, and a variety of other stressors can sway this balance (Galluzzi et al., 2018). While infected or damaged cells can be detrimental to the body, excessive cell death can also be problematic, especially in the inflammatory context (Rothlin et al., 2021). The number of dead cells, location, and cell death type substantially influence the inflammatory potential. The tumour necrosis factor (TNF), a pro-inflammatory cytokine, can induce apoptosis–considered an immunological silent cell death type–and necroptosis, an inflammatory cell death type (Murphy et al., 2013; Tanzer et al., 2021; Tanzer, 2022). Infection by many pathogens can also induce a highly inflammatory form of cell death, termed pyroptosis (Bergsbaken et al., 2009). Endogenous inhibitors of cell death, such as cellular FADD-like Il-1β-converting enzyme inhibitory protein (cFLIP), inhibitors of apoptosis (IAPs), myeloid leukemia cell differentiation protein (MCL-1) and B-cell lymphoma 2 (BCL-2) are effective (Vaux et al., 1988; Chang et al., 2002; Silke and Meier, 2013). However, overpowering cell death signals lead to excess cell death. Therefore, the efficient removal of dead cells is essential to prevent pathologic inflammation and sustain homeostasis of our bodies.
The removal of dead cells is called efferocytosis and is primarily mediated by macrophages. The efficient efferocytosis of dead cells is essential for wound healing and for containing inflammation. Macrophages undergo a programming switch towards an anti-inflammatory state with the release of interleukin 10 (IL-10) and transforming growth factor beta (TGF-β) (Voll et al., 1997). Upon sensing phosphatidylserine (PS) exposure on dead cells, macrophages initiate engulfment via LC3-associated phagocytosis and induce anti-inflammatory programming (Fadok et al., 1992; Korns et al., 2011). Many upstream events, such as ‘find-me’ signals released by the dead cell to attract macrophages, have been identified. PS receptors on macrophages such as Tyro 3, Axl and Mer (TAM), transmembrane immunoglobulin and mucin domain 1 and 4 (TIM-1 and 4), CD300a,b,f, stabilin-1 and 2, the receptor for advanced glycosylation end products (RAGE), brain-specific angiogenesis inhibitor 1 (BAI 1) are partially redundant, but overall essential for engulfment (Elliott et al., 2017). Little is known about the relationship between cell type, cell death, and efferocytosis efficiency, as well as the release of anti-inflammatory factors (Figure 1). Additionally, the regulation of engulfment and digestion and the role these processes play in reprogramming macrophages is still poorly understood. Even less is known about the mechanisms activated when efferocytosis is disrupted and pro-inflammatory signalling is triggered. The perturbation of engulfment by Rubicon deficiency, an essential member of the LC3-associated phagocytosis pathway, and digestion seen in patients with mutated deoxyribonuclease II (DNase II), generate pro-inflammatory signalling (Okabe et al., 2005; Cunha et al., 2018). To better understand these signalling pathways, further research must be conducted to uncover the events that inhibit anti-inflammatory and drive pro-inflammatory signalling during efferocytosis, which could offer therapeutic targets for treating inflammatory and infectious diseases (Figure 1).
FIGURE 1
The menu–dead cells and their inflammatory potential
Apoptosis, necroptosis and pyroptosis are the main cell death types involved in inflammation, activated by cytokines and pathogens. Necroptosis is mediated by MLKL activation, and pyroptosis is mediated via gasdermin D activation. Both are lytic cell death pathways. (Sun et al., 2012; Murphy et al., 2013; Kayagaki et al., 2015; Atkin-Smith, 2021). In contrast, apoptotic cells stay intact and active caspases degrade inflammatory molecules before their leakage into the extracellular space. Numerous reports investigate the inflammatory potential of these different cell death types by studying inflammatory factors released by dying cells (Dinarello, 1997; Orozco et al., 2019; Tanzer et al., 2020; Phulphagar et al., 2021). Pyroptosis, for example, leads to the release of many inflammatory mediators, including the highly pro-inflammatory cytokines cleaved IL-1B and IL-18 (Dinarello, 1997; Phulphagar et al., 2021). The inflammatory potential of TNF-induced apoptosis and necroptosis is less clear. Apoptotic T-cells release anti-inflammatory factors including TGF-β, IL-10 and ATP (Szondy et al., 2017). Nevertheless, in a global analysis of proteins released by these cell death types in vitro, we see prolonged production of cytokines by apoptotic cells. In contrast, during the early stages of necroptosis, dying cells release various cellular components such as lysosomal proteins (Tanzer et al., 2020).
Cell death types not only differ in their inflammatory potential based on the release of different inflammatory factors, but also differentially impact the removal of dead cells. A macrophage’s perturbed or deficient ability to detect and remove dead cell populations increases the dead cells’ inflammatory potential. Nevertheless, less focus has been placed on how different cell death pathways influence removal efficiency. The cell death type affects the recruitment of macrophages through the release of find-me signals, including metabolites, chemokines and growth factors (Ravichandran, 2010). Systematic comparisons of proteomic and metabolomic analyses of dead cell supernatants and in vivo analyses will give more insight into the recruitment ability of cells dying via different cell death pathways.
A classical hallmark of apoptosis is the exposure of PS, which is also the main trigger for engulfment. Upon apoptosis, certain flippases such as ATPase phospholipid transporting 11c (ATP11c), are inhibited and scramblases such as XK-related protein 8 (XKR8) are activated by active caspases (Nagata et al., 2016). Interestingly, necroptotic and pyroptotic cells also demonstrated increased PS staining, but due to membrane permeabilisation, this might not be specific to the outer leaflet (Zargarian et al., 2017; Atkin-Smith, 2021). Efferocytosis efficiency of necroptotic and pyroptotic cells was reduced. This could be due to the increased expression of engulfment inhibitors like cluster of differentiation 47 (CD47) and the lack of other pro-engulfment factors on necroptotic cells (Gardai et al., 2005). Another factor for the optimal stimulation of engulfment receptors could be the distribution of PS on the outer leaflet (Kern et al., 2021). Considering the significant differences in cell morphology between apoptosis, necroptosis and pyroptosis, differences in PS distribution are very likely.
Besides engulfment and recruitment, digestion efficiency will vary between different cell corpses. Digestion of smaller apoptotic bodies will likely be faster than that of large necroptotic cells (Atkin-Smith and Poon, 2017). The variable disassembly of cells could also influence their digestion. In contrast to necroptotic cells, apoptotic cells activate caspases that cleave many intracellular inflammatory components. These differences affect the reprogramming of macrophages. Consequently, macrophages engulfing necroptotic cells release increased pro-inflammatory cytokines (Zargarian et al., 2017). Comparing signalling activation between the engulfment of apoptotic versus necroptotic or pyroptotic cells could elucidate pathways important for anti-inflammatory reprogramming.
The recruitment, engulfment, digestion and inflammatory programming will also be affected by the cell type of the dying cell and the tissue-specific macrophages, such as Kupffer cells in the liver and microglia in the brain. Investigations of dead cells and macrophages within these tissues, for example, by combining laser microdissection with single-cell proteomics, will be necessary to regulate efferocytosis effectively in tissue-specific diseases (Mund et al., 2022).
When ingestion is deregulated–LC3-associated phagocytosis
Activating LC3-associated phagocytosis (LAP) is essential for anti-inflammatory reprogramming during efferocytosis (Heckmann and Green, 2019). In contrast to classical autophagy, the engulfment of corpses leads to the recruitment of LAP-specific proteins such as Rubicon and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2) to the single-membrane phagosome (Martinez et al., 2015). Recruitment of vacuolar protein sorting 34 (VPS34) to the complex induces the production of phosphatidylinositol 3-phosphate (PI3P), which is essential for LC3 binding to the phagosomal membrane (Heckmann and Green, 2019). LC3 association facilitates phagosome fusion with the lysosome and Rab-GTPases enhance phagosome maturation (Fazeli and Wehman, 2017). Consequently, the digestion efficiency of corpses and certain phagocytosed pathogens is increased, which is believed to reduce inflammation, contributing to the induction of anti-inflammatory mediators (Heckmann and Green, 2019). Cunha et al. demonstrated that perturbation of LAP by knocking out LAP-specific components, including Rubicon, induced type I interferon responses via the activation of Sting1 through unknown mechanisms (Cunha et al., 2018). This inflammatory response reduced tumour growth of a melanoma cell line via activation of T-cells. The LAP machinery could therefore serve as a target for anti-tumour therapy. Another study reported Rubicon as an inhibitor of the antiviral response by directly interacting with interferon regulatory factor 3 (Irf3) and inhibiting its dimerisation (Kim et al., 2017). Pena Martinez et al. speculated on Rubicon’s role in localising and inhibiting the NLR family pyrin domain containing 3 (NLRP3) inflammasome (Pena-Martinez et al., 2022). Another report demonstrated Rubicon interacting with p22phox, an NADPH oxidase subunit. This interaction increased reactive oxygen species (ROS) production, cytokine expression and pathogen clearance (Yang et al., 2012). In the future, it would be interesting to dissect Rubicon’s role in a LAP-dependent and independent context. Two Saudi Arabian families have been identified as harbouring Rubicon mutations, which obstruct its endolysosomal localisation, resulting in ataxia as the primary symptom in afflicted family members (Assoum et al., 2010; Seidahmed et al., 2020). More investigation into immunological abnormalities upon infection or in the context of cancer could further elucidate its function in a physiological setting.
Since the first description of LAP in 2007, many questions about LAP regulation and its physiological impact remain (Sanjuan et al., 2007). Rubicon complex recruitment to the phagosome depends on the engagement of PS receptors such as TIM4, but the exact mechanisms are unknown (Martinez et al., 2011). Regulations of LC3 binding to the phagosome downstream have also to be determined. Intact LAP is a prerequisite for efficient cargo digestion and subsequent inhibition of inflammation. However, it is unclear how LAP signalling regulates phagolysosome acidification, major histocompatibility complex (MHC) class II presentation and digestion of diverse cargo components (Romao and Munz, 2014). Future investigations that analyse ingested material and compare the signalling activation of LAP-deficient cells to that of wild-type cells could shed light on the role of LAP in macrophage reprogramming during efferocytosis.
Digestion, digestion, digestion: The leaky phagolysosome in health and disease
The last step of efferocytosis involves the digestion of the cellular corpse. The majority of dead cell digestion occurs in the phagolysosome, where pH levels are low, and active proteases, DNases and other digestive enzymes are present (Howell et al., 2003; Yang and Wang, 2021). Efficient digestion is a prerequisite for efficient efferocytosis and is essential to prevent inflammation. Two decades ago, the Nagata Lab showed that DNaseII-deficient mice died due to defective erythropoiesis induced by perturbed digestion of old erythrocytes (Kawane et al., 2001). Later, they demonstrated that transfer of DNaseII-deficient bone marrow cells induced arthritis and that macrophages were responsible for the phenotype (Kawane et al., 2010). Besides deficient digestion, deficient engulfment was also linked to disease. Increased auto-antibodies were detected in TAM-deficient mice. Lupus-like autoimmune disease features were observed upon lack of the scramblase Xkr8, which regulates PS exposure of apoptotic cells or in milk fat globule epidermal growth factor 8 (Mfg-e8)-deficient mice lacking Mfg-e8, a PS-binding protein mediating integrin binding and engulfment (Lu and Lemke, 2001; Hanayama et al., 2004; Kawano and Nagata, 2018).
The origin of inflammatory signalling upon hampered efferocytosis is challenging to define but essential for developing effective anti-inflammatory treatment. Reduced removal of dead cells leads to accumulating inflammatory and immunogenic intracellular material such as DNA in the extracellular space. Extracellular DNA is a prime example of a significant inflammatory mediator. DNA clearance is reduced during sepsis and is linked to COVID-19 severity (Aramburu et al., 2022). DNA in the extracellular space activates the B-cell response leading to increased anti-DNA antibodies in the plasma. Extracellular DNases are, therefore, essential to reduce inflammation and are heavily studied in the context of lupus (Martinez Valle et al., 2008).
Perturbed ingestion and digestion of dead cells not only lead to the release of inflammatory material in the extracellular space, but also activate inflammatory pathways within the efferocytosing cell. Several studies indicated that phagolysosomes are not entirely sealed and leakage of undigested material could trigger inflammation (Okabe et al., 2005; Cunha et al., 2018). Although it has not been experimentally demonstrated thus far, undigested DNA is believed to leak into the cytosol in DNaseII-deficient macrophages and be responsible for the secretion of inflammatory cytokines (Okabe et al., 2005). Consistently, Cunha et al. detected Sting1 activation and interferon production in mice with LAP-deficient macrophages (Cunha et al., 2018). Earlier, it was shown that transfection of DNA from apoptotic and necrotic cells induced Sting1 activation in macrophages (Ahn et al., 2012). The mechanism of DNA release from the phagolysosome is unknown. Oxidative stress has been found to induce lysosomal rupture (Terman et al., 2006). Whether this contributes to phagolysosome leakage of efferocytosing macrophages has yet to be determined. Engulfment of large-sized cell corpses could put mechanical strain on the phagolysosomes and contribute to leakage. These explanations entail phagolysosomal leakiness in efferocytosing wild-type macrophages, which do not exert pro-inflammatory signatures. How wild-type cells circumvent the pro-inflammatory response would be interesting to address. During intact efferocytosis in wild-type cells, digestion will be efficient, and leakage of undigested material will be minimal but present. Upon leakage of undigested material, mechanisms inhibiting Sting1 activation and other inflammatory pathways must be in place. How perturbed engulfment and digestion override these anti-inflammatory programs is unknown.
It is also unclear whether other molecules of dying cells, such as proteins, lipids and other metabolites leak into the cytosol and whether they are also triggering an inflammatory response. Zhang et al. demonstrated that fatty acids from engulfed apoptotic cells activated mitochondrial respiration to ensure NAD+ production, Sirtuin activation and IL-10 upregulation upon efferocytosis (Zhang et al., 2019). This implies that fatty acids exit the phagolysosome. Considering these discoveries, a particular focus should be set on the impact of different cargo on macrophage programming. Cells derived from different tissue and undergoing different cell death types will differentially activate macrophage receptors and influence macrophage programming based on cytosolic exposure of cellular debris.
Pathogens within dead cells can also access the cytosol. Certain pathogens, such as Staphylococcus aureus and Salmonella typhimurium, are directly phagocytosed by macrophages, undergo LAP and invade the cytosol through the phagolysosome (Herb et al., 2022; Mehrotra and Ravichandran, 2022). Other pathogens could not infect macrophages if it was not through another infected dead cell that serves as a Trojan horse. Efferocytosis of apoptotic cells infected by SARS-CoV-2 induced upregulation of pro-inflammatory cytokines (Salina et al., 2022). Viral RNA could thereby enter the cytosol and induce an antiviral response. While not every virus investigated induced the production of pro-inflammatory cytokines, efferocytosis of apoptotic bodies infected with the influenza A virus led to increased viral production (Atkin-Smith et al., 2020; Salina et al., 2022). Systematic examinations of signalling activated upon engulfment of dead cells infected with selected pathogens could help determine the contribution of phagolysosome leakage in various infectious diseases. In the context of viral infections, the macrophage could activate antiviral signalling and act as a new host cell by producing viral proteins. Intracellular localisation studies using proteomics and metabolomics approaches to identify peptides and metabolites of pathogens within macrophages in combination with high resolution microscopy will elucidate the understudied aspects of pathogen-macrophage interactions.
Concluding remarks
Even though efferocytosis exemplifies anti-inflammatory processes, like most immune processes, it is susceptible to perturbations. Due to its complexity, efferocytosis can be deregulated at many levels. To gain a better understanding of the precise signals that lead to the anti-inflammatory programming of macrophages, more research is necessary. The roles of various receptors in reprogramming macrophages upon efferocytosis has not been dissected precisely. To achieve this, tissue specific receptor deletion is required, as macrophage receptor expression is variable dependent on macrophage location. Additionally, macrophages that are resident in different tissues are exposed to different cell types undergoing different cell death processes. This will influence the engulfment and digestion efficiency and will pose variable metabolic challenges on macrophages. The effect of efferocytosis of dead cells with variable metabolic compositions on macrophage reprogramming has to be investigated, especially in context of disease. Also, pathogens within dead cells may be able to manipulate efferocytosis and the inflammatory status of macrophages for their own benefit. State-of-the-art technology, such as spatially resolved high-sensitive metabolomics and proteomics including the analysis of post-translational modifications, will uncover regulators of efferocytosis in vitro, in mouse models, and patient samples.
Open access statement
Open access for this article was enabled by the participation of Max Planck Digital Library.
Statements
Author contributions
MCT wrote the manuscript.
Funding
MCT was supported by the Max Planck Society for the Advancement of Science.
Acknowledgments
Many thanks to Che A Stafford and Julie S Stafford for editing the manuscript. The figure was generated with Biorender.com.
Conflict of interest
The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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References
1
AhnJ.GutmanD.SaijoS.BarberG. N. (2012). STING manifests self DNA-dependent inflammatory disease. Proc. Natl. Acad. Sci. U. S. A.109, 19386–19391. 10.1073/pnas.1215006109
2
AramburuI. V.HovingD.VernardisS. I.TinM. C. F.IoannouM.TemkinM. I.et al (2022). Functional proteomic profiling links deficient DNA clearance with increased mortality in individuals with severe COVID-19 pneumonia. Immunity55, 2436–2453.e5. 10.1016/j.immuni.2022.11.007
3
AssoumM.SalihM. A.DrouotN.H'Mida-Ben BrahimD.Lagier-TourenneC.AldreesA.et al (2010). Rundataxin, a novel protein with RUN and diacylglycerol binding domains, is mutant in a new recessive ataxia. Brain133, 2439–2447. 10.1093/brain/awq181
4
Atkin-SmithG. K. (2021). Phagocytic clearance of apoptotic, necrotic, necroptotic and pyroptotic cells. Biochem. Soc. Trans.49, 793–804. 10.1042/BST20200696
5
Atkin-SmithG. K.DuanM.ZankerD. J.LohL.NguyenT. H. O.KoutsakosM.et al (2020). Monocyte apoptotic bodies are vehicles for influenza A virus propagation. Commun. Biol.3, 223. 10.1038/s42003-020-0955-8
6
Atkin-SmithG. K.PoonI. K. H. (2017). Disassembly of the dying: Mechanisms and functions. Trends Cell Biol.27, 151–162. 10.1016/j.tcb.2016.08.011
7
BergsbakenT.FinkS. L.CooksonB. T. (2009). Pyroptosis: Host cell death and inflammation. Nat. Rev. Microbiol.7, 99–109. 10.1038/nrmicro2070
8
ChangD. W.XingZ.PanY.Algeciras-SchimnichA.BarnhartB. C.Yaish-OhadS.et al (2002). c-FLIP(L) is a dual function regulator for caspase-8 activation and CD95-mediated apoptosis. EMBO J.21, 3704–3714. 10.1093/emboj/cdf356
9
CunhaL. D.YangM.CarterR.GuyC.HarrisL.CrawfordJ. C.et al (2018). LC3-Associated phagocytosis in myeloid cells promotes tumor immune tolerance. Cell175, 429–441. 10.1016/j.cell.2018.08.061
10
DinarelloC. A. (1997). Interleukin-1. Cytokine Growth Factor Rev.8, 253–265. 10.1016/s1359-6101(97)00023-3
11
ElliottM. R.KosterK. M.MurphyP. S. (2017). Efferocytosis signaling in the regulation of macrophage inflammatory responses. J. Immunol.198, 1387–1394. 10.4049/jimmunol.1601520
12
FadokV. A.VoelkerD. R.CampbellP. A.CohenJ. J.BrattonD. L.HensonP. M. (1992). Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J. Immunol.148, 2207–2216. 10.4049/jimmunol.148.7.2207
13
FazeliG.WehmanA. M. (2017). Safely removing cell debris with LC3-associated phagocytosis. Biol. Cell109, 355–363. 10.1111/boc.201700028
14
GalluzziL.VitaleI.AaronsonS. A.AbramsJ. M.AdamD.AgostinisP.et al (2018). Molecular mechanisms of cell death: Recommendations of the nomenclature committee on cell death 2018. Cell Death Differ.25, 486–541. 10.1038/s41418-017-0012-4
15
GardaiS. J.McphillipsK. A.FraschS. C.JanssenW. J.StarefeldtA.Murphy-UllrichJ. E.et al (2005). Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte. Cell123, 321–334. 10.1016/j.cell.2005.08.032
16
HanayamaR.TanakaM.MiyasakaK.AozasaK.KoikeM.UchiyamaY.et al (2004). Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice. Science304, 1147–1150. 10.1126/science.1094359
17
HeckmannB. L.GreenD. R. (2019). LC3-associated phagocytosis at a glance. J. Cell Sci.132, jcs222984. 10.1242/jcs.222984
18
HerbM.GluschkoA.FaridA.KronkeM. (2022). When the phagosome gets leaky: Pore-forming toxin-induced non-canonical autophagy (PINCA). Front. Cell Infect. Microbiol.12, 834321. 10.3389/fcimb.2022.834321
19
HowellD. P.KrieserR. J.EastmanA.BarryM. A. (2003). Deoxyribonuclease II is a lysosomal barrier to transfection. Mol. Ther.8, 957–963. 10.1016/j.ymthe.2003.09.011
20
KawaneK.FukuyamaH.KondohG.TakedaJ.OhsawaY.UchiyamaY.et al (2001). Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver. Science292, 1546–1549. 10.1126/science.292.5521.1546
21
KawaneK.TanakaH.KitaharaY.ShimaokaS.NagataS. (2010). Cytokine-dependent but acquired immunity-independent arthritis caused by DNA escaped from degradation. Proc. Natl. Acad. Sci. U. S. A.107, 19432–19437. 10.1073/pnas.1010603107
22
KawanoM.NagataS. (2018). Lupus-like autoimmune disease caused by a lack of Xkr8, a caspase-dependent phospholipid scramblase. Proc. Natl. Acad. Sci. U. S. A.115, 2132–2137. 10.1073/pnas.1720732115
23
KayagakiN.StoweI. B.LeeB. L.O'RourkeK.AndersonK.WarmingS.et al (2015). Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling. Nature526, 666–671. 10.1038/nature15541
24
KernN.DongR.DouglasS. M.ValeR. D.MorrisseyM. A. (2021). Tight nanoscale clustering of Fcγ receptors using DNA origami promotes phagocytosis. Elife10, e68311. 10.7554/eLife.68311
25
KimJ. H.KimT. H.LeeH. C.NikapitiyaC.UddinM. B.ParkM. E.et al (2017). Rubicon modulates antiviral type I interferon (IFN) signaling by targeting IFN regulatory factor 3 dimerization. J. Virol.91, 002488–e317. 10.1128/JVI.00248-17
26
KornsD.FraschS. C.Fernandez-BoyanapalliR.HensonP. M.BrattonD. L. (2011). Modulation of macrophage efferocytosis in inflammation. Front. Immunol.2, 57. 10.3389/fimmu.2011.00057
27
LuQ.LemkeG. (2001). Homeostatic regulation of the immune system by receptor tyrosine kinases of the Tyro 3 family. Science293, 306–311. 10.1126/science.1061663
28
MartinezJ.AlmendingerJ.OberstA.NessR.DillonC. P.FitzgeraldP.et al (2011). Microtubule-associated protein 1 light chain 3 alpha (LC3)-associated phagocytosis is required for the efficient clearance of dead cells. Proc. Natl. Acad. Sci. U. S. A.108, 17396–17401. 10.1073/pnas.1113421108
29
MartinezJ.MalireddiR. K.LuQ.CunhaL. D.PelletierS.GingrasS.et al (2015). Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins. Nat. Cell Biol.17, 893–906. 10.1038/ncb3192
30
Martinez ValleF.BaladaE.Ordi-RosJ.Vilardell-TarresM. (2008). DNase 1 and systemic lupus erythematosus. Autoimmun. Rev.7, 359–363. 10.1016/j.autrev.2008.02.002
31
MehrotraP.RavichandranK. S. (2022). Drugging the efferocytosis process: Concepts and opportunities. Nat. Rev. Drug Discov.21, 601–620. 10.1038/s41573-022-00470-y
32
MundA.CosciaF.KristonA.HollandiR.KovacsF.BrunnerA. D.et al (2022). Deep Visual Proteomics defines single-cell identity and heterogeneity. Nat. Biotechnol.40, 1231–1240. 10.1038/s41587-022-01302-5
33
MurphyJ. M.CzabotarP. E.HildebrandJ. M.LucetI. S.ZhangJ. G.Alvarez-DiazS.et al (2013). The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism. Immunity39, 443–453. 10.1016/j.immuni.2013.06.018
34
NagataS.SuzukiJ.SegawaK.FujiiT. (2016). Exposure of phosphatidylserine on the cell surface. Cell Death Differ.23, 952–961. 10.1038/cdd.2016.7
35
OkabeY.KawaneK.AkiraS.TaniguchiT.NagataS. (2005). Toll-like receptor-independent gene induction program activated by mammalian DNA escaped from apoptotic DNA degradation. J. Exp. Med.202, 1333–1339. 10.1084/jem.20051654
36
OrozcoS. L.DanielsB. P.YatimN.MessmerM. N.QuaratoG.Chen-HarrisH.et al (2019). RIPK3 activation leads to cytokine synthesis that continues after loss of cell membrane integrity. Cell Rep.28, 2275–2287. 10.1016/j.celrep.2019.07.077
37
Pena-MartinezC.RickmanA. D.HeckmannB. L. (2022). Beyond autophagy: LC3-associated phagocytosis and endocytosis. Sci. Adv.8, eabn1702. 10.1126/sciadv.abn1702
38
PhulphagarK.KuhnL. I.EbnerS.FrauensteinA.SwietlikJ. J.RieckmannJ.et al (2021). Proteomics reveals distinct mechanisms regulating the release of cytokines and alarmins during pyroptosis. Cell Rep.34, 108826. 10.1016/j.celrep.2021.108826
39
RavichandranK. S. (2010). Find-me and eat-me signals in apoptotic cell clearance: Progress and conundrums. J. Exp. Med.207, 1807–1817. 10.1084/jem.20101157
40
RomaoS.MunzC. (2014). LC3-associated phagocytosis. Autophagy10, 526–528. 10.4161/auto.27606
41
RothlinC. V.HilleT. D.GhoshS. (2021). Determining the effector response to cell death. Nat. Rev. Immunol.21, 292–304. 10.1038/s41577-020-00456-0
42
SalinaA. C. G.Dos-SantosD.RodriguesT. S.Fortes-RochaM.Freitas-FilhoE. G.Alzamora-TerrelD. L.et al (2022). Efferocytosis of SARS-CoV-2-infected dying cells impairs macrophage anti-inflammatory functions and clearance of apoptotic cells. Elife11, e74443. 10.7554/eLife.74443
43
SanjuanM. A.DillonC. P.TaitS. W.MoshiachS.DorseyF.ConnellS.et al (2007). Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis. Nature450, 1253–1257. 10.1038/nature06421
44
SeidahmedM. Z.HamadM. H.AlbakheetA.ElmalikS. A.AldreesA.Al-SufayanJ.et al (2020). Ancient founder mutation in RUBCN: A second unrelated family confirms salih ataxia (SCAR15). BMC Neurol.20, 207. 10.1186/s12883-020-01761-w
45
SilkeJ.MeierP. (2013). Inhibitor of apoptosis (IAP) proteins-modulators of cell death and inflammation. Cold Spring Harb. Perspect. Biol.5, a008730. 10.1101/cshperspect.a008730
46
SunL.WangH.WangZ.HeS.ChenS.LiaoD.et al (2012). Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell148, 213–227. 10.1016/j.cell.2011.11.031
47
SzondyZ.SarangZ.KissB.GarabucziE.KoroskenyiK. (2017). Anti-inflammatory mechanisms triggered by apoptotic cells during their clearance. Front. Immunol.8, 909. 10.3389/fimmu.2017.00909
48
TanzerM. C. (2022). A proteomic perspective on TNF-mediated signalling and cell death. Biochem. Soc. Trans.50, 13–20. 10.1042/BST20211114
49
TanzerM. C.BludauI.StaffordC. A.HornungV.MannM. (2021). Phosphoproteome profiling uncovers a key role for CDKs in TNF signaling. Nat. Commun.12, 6053. 10.1038/s41467-021-26289-6
50
TanzerM. C.FrauensteinA.StaffordC. A.PhulphagarK.MannM.MeissnerF. (2020). Quantitative and dynamic catalogs of proteins released during apoptotic and necroptotic cell death. Cell Rep.30, 1260–1270. 10.1016/j.celrep.2019.12.079
51
TermanA.KurzT.GustafssonB.BrunkU. T. (2006). Lysosomal labilization. IUBMB Life58, 531–539. 10.1080/15216540600904885
52
VauxD. L.CoryS.AdamsJ. M. (1988). Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature335, 440–442. 10.1038/335440a0
53
VollR. E.HerrmannM.RothE. A.StachC.KaldenJ. R.GirkontaiteI. (1997). Immunosuppressive effects of apoptotic cells. Nature390, 350–351. 10.1038/37022
54
YangC. S.RodgersM.MinC. K.LeeJ. S.KingeterL.LeeJ. Y.et al (2012). The autophagy regulator Rubicon is a feedback inhibitor of CARD9-mediated host innate immunity. Cell Host Microbe11, 277–289. 10.1016/j.chom.2012.01.019
55
YangC.WangX. (2021). Lysosome biogenesis: Regulation and functions. J. Cell Biol.220, e202102001. 10.1083/jcb.202102001
56
ZargarianS.ShlomovitzI.ErlichZ.HourizadehA.Ofir-BirinY.CrokerB. A.et al (2017). Phosphatidylserine externalization, "necroptotic bodies" release, and phagocytosis during necroptosis. PLoS Biol.15, e2002711. 10.1371/journal.pbio.2002711
57
ZhangS.WeinbergS.DebergeM.GainullinaA.SchipmaM.KinchenJ. M.et al (2019). Efferocytosis fuels requirements of fatty acid oxidation and the electron transport chain to polarize macrophages for tissue repair. Cell Metab.29, 443–456. 10.1016/j.cmet.2018.12.004
Summary
Keywords
efferocytosis, inflammation, LC3-associated phagocytosis, cell death, phagolysosome
Citation
Tanzer MC (2023) You are what you eat and how you digest it! A discussion on inflammatory efferocytosis. Front. Cell Dev. Biol. 11:1132696. doi: 10.3389/fcell.2023.1132696
Received
27 December 2022
Accepted
31 January 2023
Published
09 February 2023
Volume
11 - 2023
Edited by
Jianke Zhang, Thomas Jefferson University, United States
Reviewed by
Hui Xiao, Shaanxi Normal University, China
Updates
Copyright
© 2023 Tanzer.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Maria C. Tanzer, tanzer@biochem.mpg.de
This article was submitted to Cell Death and Survival, a section of the journal Frontiers in Cell and Developmental Biology
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