Andreina Arias
Alonso Quiroz
Nicolás Santander
Eugenia Morselli
Dolores Busso- 1Laboratory of Nutrition, Metabolism and Reproduction, Research and Innovation Center, Program of Reproductive Biology, Universidad de Los Andes, Santiago, Chile
- 2Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Chile
- 3School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile
- 4Instituto de Ciencias de la Salud, Universidad de O’Higgins, Rancagua, Chile
- 5Department of Basic Sciences, Faculty of Medicine and Sciences, Universidad San Sebastián, Santiago, Chile
- 6IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, Chile
Cholesterol is an essential component of animal cells. Different regulatory mechanisms converge to maintain adequate levels of this lipid because both its deficiency and excess are unfavorable. Low cell cholesterol content promotes its synthesis and uptake from circulating lipoproteins. In contrast, its excess induces the efflux to high-density lipoproteins (HDL) and their transport to the liver for excretion, a process known as reverse cholesterol transport. Different studies suggest that an abnormal HDL metabolism hinders female fertility. HDL are the only lipoproteins detected in substantial amounts in follicular fluid (FF), and their size and composition correlate with embryo quality. Oocytes obtain cholesterol from cumulus cells via gap junctions because they cannot synthesize cholesterol de novo and lack HDL receptors. Recent evidence has supported the possibility that FF HDL play a major role in taking up excess unesterified cholesterol (UC) from the oocyte. Indeed, genetically modified mouse models with disruptions in reverse cholesterol transport, some of which show excessive circulating UC levels, exhibit female infertility. Cholesterol accumulation can affect the egg´s viability, as reported in other cell types, and activate the plasma membrane structure and activity of membrane proteins. Indeed, in mice deficient for the HDL receptor Scavenger Class B Type I (SR-B1), excess circulating HDL cholesterol and UC accumulation in oocytes impairs meiosis arrest and hinders the developmental capacity of the egg. In other cells, the addition of cholesterol activates calcium channels and dysregulates cell death/survival signaling pathways, suggesting that these mechanisms may link altered HDL cholesterol metabolism and infertility. Although cholesterol, and lipids in general, are usually not evaluated in infertile patients, one study reported high circulating UC levels in women showing longer time to pregnancy as an outcome of fertility. Based on the evidence described above, we propose the existence of a well-regulated and largely unexplored system of cholesterol homeostasis controlling traffic between FF HDL and oocytes, with significant implications for female fertility.
Introduction
Overview of Cholesterol Biochemistry and Whole-Body Metabolism
Cholesterol, the principal sterol in animals, comprises a four-ring structure and a single hydroxyl group attached to one of the rings (Cortes et al., 2013). This essential component of cell membranes and precursor of steroid hormones, bile acids, and vitamins, among other molecules, exists in two forms: free or unesterified cholesterol (UC) and cholesteryl ester (CE) or esterified cholesterol (Luo et al., 2020). The first is an amphipathic, active form present in cell membranes. The second inactive form results from esterification of UC with fatty acids for storage in lipid droplets (LD) or lipoproteins.
Cholesterol is synthesized by almost every cell type and provided by the diet. The liver delivers both endogenously synthesized and exogenously acquired cholesterol to the bloodstream for its distribution to other tissues by secreting lipoproteins. These specialized particles solubilize lipids in blood and allow their bidirectional exchange with cells (Salter and Brindley, 1988). Lipoproteins are classified, according to their density, into chylomicrons, very-low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL). Each lipoprotein class contains different proportions of lipids and proteins (apolipoproteins) that determine their structure, specificity towards specific receptors and functional properties (Salter and Brindley, 1988). Apolipoprotein B (Apo B) is the principal protein component of LDL and VLDL. This large and hydrophobic protein assembles with lipids in the endoplasmic reticulum (ER) of liver or intestinal cells (Jonas et al., 2008). Smaller apolipoproteins (e.g., Apo A1, Apo A2, Apo E) have much smaller molecular masses, they can solubilize in blood, acquire lipids, and modulate the activity of plasma enzymes (Jonas et al., 2008). Although lipoproteins have mainly been studied in plasma, these particles are also present in other body fluids such as cerebrospinal, amniotic, and follicular fluid (FF), etc (Blackett and McConathy, 1982; Suchanek et al., 1988; Mahley, 2016).
In different tissues, a low cholesterol level promotes its own synthesis and uptake from circulating lipoproteins, whereas its excess induces efflux to HDL and excretion through the liver, the key organ regulating cholesterol metabolism (Luo et al., 2020). The mechanism of cholesterol mobilization from extrahepatic tissues to the liver for excretion is denominated reverse cholesterol transport (Luo et al., 2020). This process starts with the efflux of UC and phospholipids from the plasma membrane (PM) to Apo AI via ATP-binding cassette transporters A1 (ABCA1) (Brooks-Wilson et al., 1999). These lipid-poor nascent HDL particles travel in the bloodstream to other tissues and serve as acceptors for UC and other lipid classes (Phillips, 2014). Concomitantly, UC at the HDL surface is esterified by the enzyme Lecithin: Cholesterol Acyl Transferase (LCAT) using fatty acids from phospholipids and stored as CE in the lipoprotein core, along with other neutral lipids like triglycerides and hydrophobic vitamins (Frohlich et al., 1982). Mature, lipid-rich HDL mediate the efflux of additional UC cholesterol from extrahepatic cells via ABCG1 and finally bind to the hepatic non-endocytic Scavenger Receptor class B type 1 (SR-B1), which takes up CE for catabolism into bile acids or direct biliary excretion (Acton et al., 1996).
In addition to the liver, SR-B1 is also expressed in cells that require high amounts of cholesterol for their function, such as steroidogenic cells and macrophages. In macrophages, SR-B1 provides the cell with cholesterol to regulate phagocytic functions, which is essential for infection and tissue homeostasis responses. In steroidogenic cells of the adrenal gland, the ovaries and the testis, SR-B1 takes up CE from circulating HDL, which is then transported to mitochondria. Mitochondrial enzymes of the P450 family catalyze the conversion of cholesterol into steroid hormones (e.g., corticoids in the adrenal gland, estrogen and progesterone in the ovary, and testosterone in the testis).
Cholesterol Cellular Distribution and Homeostatic Processes
UC is distributed unevenly among membranes from different cellular organelles (Ikonen and Zhou, 2021). This distribution seems to depend on the relative affinity of cholesterol for the lipids composing each membrane. Cholesterol co-localizes preferentially with phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin, the major constituents of the PM. Thus, the proportion of UC to total lipids is high (near 40% of total lipids) in the PM and very low (less than 5%) in the ER and mitochondria. Due to the unique biophysical properties of cholesterol, its level determines the fluidity and heterogeneity of components at the PM (Ikonen and Zhou, 2021). Indeed, cholesterol-rich specialized microdomains, including caveolae, tetraspanin-enriched microdomains and lipid rafts, are crucial for regulating signaling pathways by restricting the localization and activity of specific proteins in the cell membrane.
Different convergent regulatory mechanisms maintain cell cholesterol levels within appropriate ranges because excess UC (levels above the physiological set point) is cytotoxic (Luo et al., 2020). Cholesterol accumulation in the PM induces the redistribution of this lipid to intracellular membranes and activates homeostatic responses to reduce cholesterol synthesis and uptake (Lange et al., 2014). Sterol regulatory element-binding proteins (SREBPs) are key players controlling cholesterol synthesis in the ER (Cortes et al., 2013). The active domains of these membrane-bound transcription factors are released proteolytically to enter the nucleus and activate genes promoting cholesterol synthesis and uptake. SCAP (SREBP cleavage-activating protein) is a sterol-regulated escort protein that transports SREBPs from their site of synthesis in the ER to their site of cleavage in the Golgi. When cholesterol levels are low, SREBPs upregulate cholesterol biosynthesis and uptake by increasing the transcription of the rate-limiting enzyme 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) and the PM LDL receptor (LDLR), respectively. On the other hand, when cholesterol levels are high, the cells upregulate the transcription of ABCA1 and ABCG1 and induce the degradation of cholesterol receptors at the PM (Acton et al., 1996).
Another mechanism to prevent damage by UC is the esterification of this lipid by sterol O-acyltransferase, also known as acyl-CoA cholesterol acyltransferase (ACAT), residing in the ER and storage of inactive CE within the hydrophobic nucleus of LD (Chang et al., 1997). In these organelles, fatty acids are also stored in triglycerides, an important source of ATP production via β-oxidation (Geltinger et al., 2020).
Cell Toxicity by UC Excess: Proposed Mechanisms
Although the precise mechanism of UC cytotoxicity is unclear, this lipid can disturb the fluidity of membranes, disrupt lipid rafts, form cytoplasmic crystals and activate different signaling pathways, all of which can reduce the cell´s viability (Tabas, 1997).
An abnormal proportion of cholesterol at the outer leaflet of the PM can reduce its fluidity and may block the function of signaling proteins that reside in lipid rafts. In artificial monolayers composed of a mixture of cholesterol, phosphatidylcholine and sphingomyelin, a rise in the percentage of cholesterol (over 33%) impedes the formation of membrane subdomains (Milhiet et al., 2002). In the adipocyte cell line 3T3-L1, increased cholesterol in the PM dysregulates the insulin-dependent glucose transport system by reducing the amount of 4,5-bisphosphate (PIP(2))-regulated filamentous actin (F-actin) structures, finally impairing Glut-4 functionality and causing insulin resistance (Bhonagiri et al., 2011).
Consistently, membrane cholesterol concentration changes in mature neurons affect their susceptibility to cell death. In young hippocampal rat neurons, increased amounts of cholesterol in the cell membrane promote beta-amyloid-dependent calpain activation, leading to the generation of the cytotoxic tau fragment (Nicholson and Ferreira, 2009). Conversely, in mature neurons, when the cholesterol concentration in the membrane is pharmacologically reduced, calpain activation and therefore tau production is blunted, promoting cell survival (Nicholson and Ferreira, 2009). Notably, cholesterol accumulation in membranes different from the PM can also affect cell viability. In liver and neurodegenerative diseases, the chronic accumulation of cholesterol in mitochondrial membranes modulates their permeability and the release of intermembrane proteins such as Bax that activate death-inducing caspases (Montero et al., 2010). This effect of cholesterol on mitochondrial membrane permeability seems to involve a reduction of GSH transport into the mitochondrial matrix; this reduced GSH availability results in the increased generation of ROS by cell death stimuli such as TNF, hypoxia or Aβ (Montero et al., 2010).
Another mechanism of UC-mediated cytotoxicity is cholesterol crystallization in LD and PM, identified in different cell types, from phagocytes to hepatocytes (Marí et al., 2006; Duewell et al., 2010; Shu et al., 2018). During UC transport for storage in LD, the concentration of this lipid may exceed the ability of phospholipids in the LD membrane to solubilize it and cause the precipitation of cholesterol monohydrate crystals (Marí et al., 2006). Cholesterol crystals induce an inflammatory response, both in mice and humans, which is associated with the activation of the NLRP3 inflammasome and may lead to lysosomal damage, finally inducing necrosis and programmed cell-death pathways such as pyroptosis, a process characterized by inflammasome activation and secretion of inflammatory cytokines (Duewell et al., 2010; Rajamäki et al., 2010; Wree et al., 2014; Favor et al., 2021). In bone-marrow-derived macrophages, the binding of cholesterol crystals to the cell surface extracts cholesterol from the PM and promotes an inflammatory response that culminates in PM damage and necrotic cell death (Shu et al., 2018). Interestingly, macrophages exposed to increasing concentration of UC also show intrinsic and extrinsic induction of apoptosis, with a fraction of the cells showing biochemical, morphological and molecular indicators of apoptosis activation, including DNA fragmentation, externalization of phosphatidylserine at the PM, activation of pro-apoptotic pathways, i.e., Fas/FasLigand, Bax-dependent cytochrome C release from the mitochondria and subsequent caspase 9 activation (Yao and Tabas, 2000; Yao and Tabas, 2001). Thus, cellular UC overload does not affect a single cell death pathway but instead activates different mechanisms that converge to cell death. Interestingly, apoptosis in macrophages can be diminished by the depletion of SR-B1 (Galle-Treger et al., 2020).
Finally, UC accumulation can promote cell toxicity by forming oxysterols, some of which promote cell death in diverse cell types from different species (Vejux et al., 2020). In rats fed diets enriched in fat and cholesterol, oxysterols promote liver toxicity by impairing mitochondrial function and driving apoptosis in hepatocytes, a mechanism potentially involved in the progression from non-alcoholic fatty liver disease to hepatic steatosis (Bellanti et al., 2018). Interestingly, 25-hydroxycholesterol and 7-Ketocholesterol activate oxiapoptophagy, a type of cell death associated with oxysterol-mediated induction of oxidative stress and characterized by features of both apoptosis and autophagy, that has been associated with the development of different diseases ranging from atherosclerosis to neurodegeneration (Anderson et al., 2020; You et al., 2021).
Lipoprotein Cholesterol as a Substrate for Ovarian Steroidogenesis
The implications of cholesterol for female reproduction have been suggested by different studies in various species (Skoblina et al., 1981; Shim et al., 2002; Bełtowski and Semczuk, 2010; Fujimoto et al., 2010; van Montfoort et al., 2014). Cholesterol is indispensable as a substrate for steroid hormone synthesis in the mammalian ovarian follicle. This structure is organized into two compartments, separated by a basal lamina: the outer compartment comprising the vascularized thecal cells and the inner, nonvascular compartment containing granulosa cells, the oocyte and cumulus cells, and the antrum filled with FF (Fahiminiya and Gérard, 2010). During steroidogenesis, follicular cells use cholesterol from different sources, i.e., local synthesis and uptake from lipoproteins in FF, and receptors for different lipoprotein classes are expressed in a cyclic and well-concerted manner in specific follicular cells to maximize steroid hormone production. FF is the product of granulosa secretions and small proteins and particles that filter from the blood through the basal lamina (Shalgi et al., 1973). It provides a rich microenvironment, composed of hormones, signaling factors, metabolites and nutrients, that supports oocyte development during follicular development (Revelli et al., 2009).
HDL is the major lipoprotein present in FF from different species, including bovine (Brantmeier et al., 1987), porcine (Chang et al., 1976), equine (Le Goff, 1994) and humans (Simpson et al., 1980); larger lipoproteins are barely detectable in antral follicles from those species. Existing evidence suggests that FF HDL mainly originate from plasma (Le Goff, 1994; Jaspard et al., 1997; Quiroz et al., 2020). The first evidence to support this came from early work from Le Goff and collaborators who characterized FF HDL in mares (Le Goff, 1994). In their study, plasma and FF lipoproteins were isolated by density gradient ultracentrifugation and characterized according to size, density, lipid content and electrophoretic mobility. The authors concluded that FF contained smaller HDL subtypes, with higher ratios of cholesterol/phospholipid and CE/UC than plasma. They hypothesized that larger plasma HDL particles are excluded by the basal lamina pores and smaller HDL permeate into the antrum and are metabolically transformed in the follicle to enhance reverse cholesterol transport. Consistently to what was described in equine, human FF were reported to exhibit a different composition compared to circulating HDL (Jaspard et al., 1997). Compared to plasma HDL, human FF HDL seem to be predominantly smaller, with less UC (and a higher esterification rate), more phospholipids, and a higher ApoA-IV/ApoA-I ratio, supporting the idea that HDL are either of different origin or subject to remodeling in the follicular antrum. We recently provided the first experimental evidence demonstrating that circulating HDL reach the antrum (Quiroz et al., 2020). In an ovarian cross-transplantation experiment in mice, ApoA-I was immunodetected on the surface of granulosa cells facing the antrum and within the antral cavity of ApoA-I deficient (ApoA-I KO) ovaries transplanted into wild type (WT) mice. By contrast, ApoA-I was not detected in the antrum or granulosa cells of WT mouse ovaries transplanted into Apo-AI KO females. Together, the results of our study suggested that mouse FF HDL originate from plasma. Unfortunately, we were unable to perform biochemical analyses to describe the properties of mouse FF HDL due to sampling limitations derived from the minimal volume of FF recovered in these animals. Intriguingly, ApoA-I mRNA and protein were detected in granulosa cells from chicken and human ovaries, opening the possibility that at least some FF HDL may be of intraovarian origin (Hermann et al., 1998; Choi et al., 2010).
FF HDLs, rich in esterified cholesterol, are poor substrates for steroid synthesis (Parinaud et al., 1987). Thus, during the follicular stage, thecal cells incorporate cholesterol from blood HDL and synthesize androgens (androstenedione and testosterone) which cross the basal membrane and fuel estrogen production by granulosa cells in the developing follicle. After the LH surge, the follicular basal membrane becomes more permeable to larger lipoproteins which enhance cholesterol availability to support the active production of steroids during ovulation (Simpson et al., 1980; Cherian-Shaw et al., 2009). Indeed, FF cholesterol concentrations are positively correlated with follicular size, and the proportions of LDL and VLDL in preovulatory follicles increase gradually (Argov et al., 2004). The LH rise also activates the SREBP/SCAP system and induces de novo cholesterol biosynthesis in mouse granulosa cells (Nakanishi et al., 2021). After ovulation, the follicle becomes vascularized and follicular cells mobilize stored CE and upregulate the expression of lipoprotein receptors (e.g., VLDLR, LDLR, SR-B1 and LRP8) (Argov et al., 2004; Cherian-Shaw et al., 2009). In the corpus luteum, luteal cells use cholesterol obtained by the novo biosynthesis and from plasma LDL and HDL to rapidly increase progesterone synthesis. In most species, SR-B1 is mainly expressed in theca and corpora lutea cells to provide cholesterol as the starting substrate for steroidogenesis (Miranda-Jiménez and Murphy, 2007; Cherian-Shaw et al., 2009; Lai et al., 2013; Chang et al., 2017). The LDLR expression in granulosa cells is mostly extinguished as luteinization progresses (Cherian-Shaw et al., 2009). By contrast, luteinization causes upregulation of SR-BI expression and posttranslational modifications that favor its insertion in luteal cell membranes (Miranda-Jiménez and Murphy, 2007; Cherian-Shaw et al., 2009). Experiments using primary cultures of rat ovarian granulosa showed that in luteal cells SR-B1 is less stringently regulated than LDLR. Hence, its increasing expression as luteinization progresses helps these cells evade the cholesterol homeostatic mechanisms and ensure sufficient cholesterol acquisition for steroid hormone production (Lai et al., 2013).
Studies by the group of A Rodriguez suggest that SR-B1 is relevant for steroidogenesis in human granulosa cells during the periovulatory stage (Velasco et al., 2006). SR-B1 expression in granulosa cells isolated from mature follicles obtained during oocyte aspirations in vitro fertilization (IVF) procedures shows positive associations with plasma estrogen levels, the number of oocytes retrieved, and fertilization rates (Velasco et al., 2006). Further support for this role came from manipulations of human granulosa cells in vitro by the same group. Indeed, reduction of SR-B1 levels using siRNA led to impaired progesterone secretion in these cells, even after stimulation with forskolin (Kolmakova et al., 2010). In addition, single nucleotide polymorphisms (SNP) in the SCARB1 gene, which codes for SR-B1 in humans, are associated with progesterone levels in FF and viable pregnancy outcomes in women subjected to IVF (Yates et al., 2011). Altogether, the evidence indicates that lipoprotein-mediated cholesterol uptake is relevant for steroidogenesis in theca, granulosa, and luteal cells.
As mentioned above, LDL and larger lipoproteins are not abundant in FF. LDL are prone to forming oxidized LDL (oxLDL), which induce multiple metabolic and functional disturbances in cells, as shown by numerous studies in different pathologies (Mineo, 2020; Deng et al., 2022). It may be hypothesized that exposure of oocytes in preovulatory follicles to high LDL levels is avoided as a protective mechanism to prevent their damage by oxidative stress. However, apoB-containing lipoproteins are secreted by human granulosa cells and show a positive relationship with improved fertility parameters after in vitro fertilization (IVF) (Gautier et al., 2010). These results suggest that large lipoproteins may be locally assembled and secreted into FF. In the last years, numerous studies have agreed in demonstrating that fertility is compromised in women with high body mass index, and that one of the drivers of this dysfunction is bad oocyte quality (Silvestris et al., 2018). Women with obesity are exposed to systemic chronic oxidative stress and exhibit high levels of circulating oxLDL (Korac et al., 2021). In this regard, exposure of preovulatory follicles to high levels of oxLDL in women with obesity and the resulting adverse effects on the oocyte viability and function may be one of the mechanisms contributing to infertility in obesity. Additional studies from different perspectives are required to underscore the complex mechanisms through which an adverse cholesterol metabolism may hinder fertility in women with obesity (Gonzalez et al., 2022).
Relationship Between FF HDL and Oocyte Quality and Developmental Potential
Early knowledge on the role of HDL particles for oocyte quality and developmental potential was provided by Fujimoto, Browne and collaborators, who analyzed FF in assisted reproductive technology (ART) procedures (Fujimoto et al., 2010). In those studies, FF HDL cholesterol concentration and particle size were negatively correlated with IVF outcomes and preimplantation embryo quality parameters, including fragmentation and symmetry (Browne et al., 2008; Browne et al., 2009; Kim et al., 2017a). The authors proposed that alterations in oocyte intracellular cholesterol homeostasis due to abnormal HDL may hinder embryo quality (Fujimoto et al., 2010). It is interesting to note that those studies only provided information on total cholesterol, so associations between UC or esterified cholesterol and the parameters mentioned above cannot be inferred from these studies. Similar to plasma HDL, FF HDL contain other molecules that may be important for the physiology of oocytes and early embryos, such as sphingosine 1-phosphate (S1P), carotenoids, vitamins and antioxidant enzymes (Schweigert, 2003; Kim et al., 2017b). Indeed, analyses of FF HDL obtained during ovum pickup preceding ART procedures show that specific molecular components from HDL of different sizes relate to preimplantation embryo quality (Schweigert, 2003; Browne et al., 2009; Kim et al., 2017b). In this context, HDL-associated molecules that may be considered potential predictors of successful IVF include γ-tocopherol, which correlates with less embryo fragmentation (Browne et al., 2009); FF-paraoxonase-arylesterase activity, a positive predictor of embryo cell number (Kim et al., 2017b); phosphatidylcholine, positively correlated with embryo cleavage (Wallace et al., 2012); and S1P, associated with good embryo quality and a better chance of clinical pregnancy (Garrido et al., 2000). In recent work, FF HDL from modified natural IVF cycles showed a positive relationship between FF HDL anti-inflammatory properties and the developmental potential of the oocyte (Jia et al., 2020). However, the relationship with pregnancy success was not significant in that study. There are some limitations in studies using samples from ART procedures, e.g., inclusion of a low number of patients with variable clinical characteristics, determination of a reduced group of molecules in each study and transfer of more than one embryo, all of which difficult direct embryo-pregnancy relationships. However, they provide valuable evidence that supports the idea that the close interaction of FF HDL and the developing oocyte is essential for its developmental potential through endocrine and non-endocrine mechanisms. Different functions attributed to FF HDL, including reverse cholesterol transport, antioxidant capacity and signaling in follicular cells and the oocyte, and provision of cholesterol as a substrate for steroid synthesis during the periovulatory phase, may be necessary for the physiology of oocytes and early embryos. The following sections will discuss how FF HDL may contribute to oocyte cholesterol homeostasis.
Cholesterol Content and Distribution in Oocytes and Eggs
Mouse oocytes and preimplantation embryos are deficient in cholesterol biosynthesis, as shown by the low expression of transcripts encoding enzymes of the cholesterol synthetic pathway and the levels of radio-labeled cholesterol detected after providing denuded oocytes with labeled acetate (Su et al., 2008). Oocytes are most probably unable to take up cholesterol from the surrounding extracellular microenvironment because they don´t express lipoprotein receptors. Indeed, neither HDL cholesterol receptor (SR-B1) nor LDLR are expressed in mammalian oocytes (Sato et al., 2003; Trigatti et al., 2003; Quiroz et al., 2020). Since they cannot synthesize or take up cholesterol by themselves, oocytes rely on the provision of cholesterol from granulosa or cumulus cells. Data suggest that cholesterol is transported from cumulus cells to oocytes across the zona pellucida via gap junctions, intercellular channels that allow passage of small molecules between cells (Anderson and Albertini, 1976). These structures provide the physical basis for cumulus-to-oocyte cholesterol transfer within the follicle. Interestingly, oocytes stimulate cholesterol biosynthesis in cumulus cells through paracrine growth factors such as bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) (Su et al., 2008).
The cholesterol content in the oocyte seems to modulate maturation, fertilization, activation and embryo development (Skoblina et al., 1981; Shim et al., 2002; Buschiazzo et al., 2008; Buschiazzo et al., 2013; Marco-Jimenez et al., 2014; Yesilaltay et al., 2014). Buschiazzo et al. showed that cholesterol depletion with methyl-beta-cyclodextrin (MβCD) in the mouse oocyte PM reduced in vitro fertilization and egg activation rates, which was associated with alterations in the structure of PM microdomains (Buschiazzo et al., 2013). MβCD-treated oocytes also showed raft-associated -but not non-raft-associated- protein depletion. Interestingly, cholesterol repletion using cholesterol-loaded MβCD (MβCD-Chol) almost completely restored fertility (Buschiazzo et al., 2013). Recent studies in farm animals showed that a significant proportion of cholesterol is removed from the PM during vitrification, a well-known cryopreservation method used to preserve female gametes (Buschiazzo et al., 2017; Atalla et al., 2018; Chen et al., 2019). This method, applied in farm animal production, human-assisted reproduction and protection of endangered species, is still considered inefficient because it affects the oocytes’ viability and fertilization capacity. Studies using bovine, ovine and porcine eggs demonstrated that rising UC cholesterol levels transiently during vitrification preserves oocyte lipid rafts organization and quality, resulting in improved maturation, viability, fertilization, and early development rates after thawing (Buschiazzo et al., 2017; Atalla et al., 2018; Chen et al., 2019). In a recent paper, Hao et al. showed that the level of JUNO, a glycosylphosphatidylinositol (GPI)-anchored protein expressed on the egg surface that is essential for female fertility (Bianchi et al., 2014), was significantly reduced in bovine eggs by vitrification (Hao et al., 2021). The successive treatment of MβCD-Chol/MβCD before/after cryopreservation preserved oocyte JUNO levels and significantly improved fertilization capacity. The GPI anchor allows proteins to anchor to lipid rafts (Seong et al., 2013). Altogether, this information suggests that changes in cholesterol levels at the egg PM may affect the activity of membrane proteins, especially those localized in rafts.
In a recent study cholesterol depletion from the PM of Xenopus oocytes induced the activation of the heterologously expressed rat transient receptor potential vanilloid 4 (TRPV4) (Lakk et al., 2021). TRPV4 is a mechanosensitive nonselective cation channel, not localized in PM microdomains, that transduces mechanical stimuli, e.g., membrane strain, shear flow, swelling, and thermal stimuli, into calcium (Ca2+) signals that control a wide range of downstream signaling pathways (Rosenbaum et al., 2020). Ca2+ homeostasis is particularly relevant in mammalian eggs because intracellular Ca2+ oscillations trigger activation and embryo development after fertilization. Cytoplasmic Ca2+ levels are strictly regulated before sperm-egg fusion to prevent egg parthenogenetic activation, fragmentation and apoptosis (Gordo et al., 2002; Ozil et al., 2005). Multiple ion transporters regulate intracellular Ca2+ levels (iCa2+) and storage of Ca2+ in the ER, as reviewed in (Wakai et al., 2013). We showed that loading WT mouse eggs with MβCD-Chol induced an elevation of iCa2+ and a reduction in maturation promoting factor and MAPK activities, followed by progression to meiotic stages beyond MII, extrusion of a second polar body, and parthenogenic cleavage (Yesilaltay et al., 2014). Recent work from R. Fissore´s laboratory demonstrated that at least three divalent-permeable ion channels expressed in the egg PM regulate Ca2+ influx during fertilization-induced oscillations: the transient receptor potential vanilloid member 3 (TRPV3), the transient receptor potential cation channel subfamily M, member 7 (TRPM7) and the T-type channel CaV3.2 (Bernhardt et al., 2018; Mehregan et al., 2021). It may be hypothesized that cholesterol in the egg PM. Activates Ca2+ channels and therefore its levels need to be low to maintain MII arrest. Although this hypothesis needs to be demonstrated experimentally, several observations support this idea: 1) an increase in the proportion of cholesterol in eggs activates PM Ca2+ channels; 2) the expression of SOATs increases during maturation (progression from GV to MII stages); and 3) cholesterol levels decrease in mouse oocytes during this period (Li et al., 2020).
The mechanisms regulating cholesterol homeostasis by esterification and storage in oocyte LD are largely unknown. In bovine eggs, removing PM cholesterol with MβCD significantly reduced the density of LD in the cell cortex (Buschiazzo et al., 2017). On the other hand, pulse-chase experiments using fluorescent BODIPY-labelled cholesterol demonstrated the internalization of the probe and its accumulation in cytoplasmic structures resembling LD (Buschiazzo et al., 2017). This evidence suggests that hydrolysis of CE and esterification of UC may be used by oocytes to control cholesterol content in membranes, as observed in other cell types (Ouimet and Marcel, 2012). However, more studies are needed to understand how LD could help to modulate cholesterol levels in oocytes. Species-specific mechanisms need to be considered because lipid levels and LD distribution are highly variable in different animals (Bradley and Swann, 2019). Compared to human or mouse oocytes, the oocytes from dogs, pigs, cows and sheep contain a much higher lipid concentration and LD that are detectable by transmission microscopy (Bradley and Swann, 2019).
Link Between Infertility and UC: Lessons From Genetically Modified Mice
Evidence from genetically modified mice suggests that disruptions in the HDL metabolism may affect female fertility (Table 1). One of the best described mouse models linking disruptions in HDL metabolism and infertility is the SR-B1 knock out (SR-B1 KO) mouse, generated in M. Krieger´s laboratory 25 years ago to understand the relationship between reverse cholesterol transport and atherosclerosis risk (Rigotti et al., 1997). SR-B1 KO mice display distinctively large HDL enriched in apolipoprotein E (ApoE) and UC and an increased incidence of pathologies in critical physiological systems, including high atherosclerosis susceptibility (Rigotti et al., 1997; Rigotti et al., 2003; Hoekstra et al., 2010; Liu et al., 2021). Unexpectedly, during the generation of SR-B1 KO mice females were found to be sterile (Trigatti et al., 1999). SR-B1 KO ovaries showed smaller corpora lutea and suboptimal luteal steroid production after ovulation, which agrees with the high expression of SR-B1 in luteal cells (Miranda-Jiménez and Murphy, 2007; Jiménez et al., 2010). Despite this endocrine disorder, SR-B1 KO animals undergo unaltered estrus cycles. They ovulate normally, both naturally and after superovulation, so insufficient hormone synthesis cannot explain SR-B1 KO female infertility (Trigatti et al., 1999). In the first paper reporting the reproductive phenotype of SR-B1 KO females, the authors described that around 60% of the early embryos produced by mating with WT males showed an abnormal, non-refractile morphology, “reminiscent of that seen in embryos mechanically damaged during pronuclear injection” (Trigatti et al., 1999). Those embryos did not develop further and were suspected to be dead. The eggs harvested after superovulation of SR-B1 KO females also showed compromised viability, although in a lower proportion (around 30%). Unexpectedly, SR-B1 was not detected in oocytes.
TABLE 1. Fertility and cholesterol levels in plasma and eggs from genetically modified mice with disruptions in HDL metabolism.
Two alternative mechanisms were proposed to explain infertility in SR-B1 KO females (Trigatti et al., 1999). The first involved deficiencies in the provision of cholesterol or other HDL-transported lipids to support follicular cell functions due to the lack of SR-B1 in the ovary itself. This possibility was ruled out by a straightforward experiment involving bilateral transplantation of SR-B1 KO ovaries into immunocompromised ovariectomized WT females (Miettinen et al., 2001). Two to three weeks after transplantation, around 80% of the females with SR-B1 negative ovaries mated with WT males delivered healthy heterozygous pups, showing that the lack of SR-B1 in the ovary was not the cause of infertility in SR-B1 KO females. The second potential mechanism for SR-B1 KO female sterility comprised a negative effect on egg viability of large, dysfunctional HDLs circulating in those animals (Trigatti et al., 1999). Existing evidence suggests that structural and functional properties of FF HDL influence follicle and oocyte biology. To test this hypothesis, M. Krieger and his team used different genetic and pharmacologic strategies to modify the structure of circulating HDL in SR-B1 KO females and subsequently examined their fertility by mating them with WT males (Miettinen et al., 2001). They generated ApoA-I/SR-B1 double KO (dKO) females with smaller, less cholesterol-enriched HDL than those in SR-B1 KO females. HDL from ApoA-I-deficient mice had normal size but altered composition: more UC and less CE. After mating them with WT males, around 40% of dKO females became pregnant and delivered healthy pups, supporting the idea that normalization of HDL structure may restore fertility in SR-B1 KO females. As an additional strategy to test this possibility, researchers from the same group treated SR-B1 KO females with the powerful cholesterol-lowering drug probucol (Barnhart et al., 1970; Miettinen et al., 2001). Total cholesterol (TC) levels were reduced by approximately 50% and the UC:TC ratio was normalized in SR-B1 KO females after administration of 0.5% probucol in the diet for 3 weeks. This intervention restored fertility rapidly and completely: as soon as one week after starting probucol administration, females became pregnant and delivered healthy, normal-sized litters. In a subsequent paper, Krieger´s group explored the effect of overexpressing SR-B1 in the liver of SR-B1 KO females on HDL structure and female fertility (Yesilaltay et al., 2006). They found that hepatic SR-BI expression using adenovirus-mediated transient expression and stable transgenesis completely restored female fertility associated with correcting the two main structural defects of SR-B1 KO HDL, their abnormally large size and high UC:TC ratio. Apparently, reducing the proportion of UC in HDL in these animals seemed to be effective at reversing infertility, similarly to the effect in probucol-treated SR-B1 KO females.
In collaboration with M Krieger´s group, we compared the cholesterol content and distribution in WT and SR-B1 KO eggs using filipin, a fluorescent UC-specific probe. Uniform staining with brighter puncta, suggestive of intracellular UC deposits, was observed in eggs from mice of both genotypes (Yesilaltay et al., 2014). However, SR-B1 KO eggs showed higher filipin fluorescence intensity (∼68%), indicative of UC accumulation (Yesilaltay et al., 2014). In addition, ̴ 73% of SR-B1 KO eggs showed spontaneous progression beyond MII arrest after superovulation (Yesilaltay et al., 2014). Also, some of them were found to be dead (∼19%), in agreement with previous results (Trigatti et al., 1999). These results, together with our data showing that artificially induced cholesterol excess using MβCD-Chol in mouse WT eggs promoted their spontaneous activation and parthenogenic cleavage, suggest that cholesterol enrichment may be a cause of exit from MII arrest independent of fertilization (Yesilaltay et al., 2014). Furthermore, those results also link cholesterol excess in SR-B1 KO eggs with female infertility in this model.
We recently showed that cholesterol accumulation in SR-B1 KO oocytes starts at the antral stage of follicular development when oocytes begin to be exposed to FF HDL (Quiroz et al., 2020). In that study, FF HDL were detected in the antrum of SR-B1 KO follicles using ApoA-I indirect immunofluorescence. Although the size and composition of FF HDL could not be characterized due to obvious limitations in the volume of mouse FF samples, cholesterol accumulation in antral oocytes from SR-B1 KO mice is consistent with the hypothesis that disruptions in HDL structure and function may affect egg quality and developmental potential (Browne et al., 2008; Browne et al., 2009; Kim et al., 2017a; Jia et al., 2020). Indeed, probucol administration to SR-B1 KO females, a treatment previously shown to lower HDL UC levels and restore fertility in these mice (Miettinen et al., 2001), normalized UC levels in both immature oocytes and ovulated eggs (Quiroz et al., 2020). The normalization of UC cholesterol levels in gametes from SR-B1 KO females was also achieved by incubating cumuli-denuded eggs with HDL purified from WT plasma, suggesting that efflux of excess cholesterol from eggs to functional HDL may help to maintain cholesterol levels within a physiological range in the gamete. In contrast, incubation of HDL purified from SR-B1 KO plasma with WT eggs did not result in evident lipoprotein-to-gamete transfer of UC. Thus, cholesterol excess in eggs from SR-B1 KO females is probably not caused primarily by UC transfer from FF HDL but due to inefficient efflux to abnormal HDL in SR-B1 KO FF. Although this in vitro assay using denuded eggs and purified plasma HDL does not recapitulate what occurs in the ovarian follicle precisely, it suggests that FF HDL may play a key role in cholesterol efflux to maintain cholesterol homeostasis in oocytes. The possibility that the fertilizing ability and developmental potential of SR-B1 KO eggs is restored after exposure to WT HDL is currently being evaluated in our laboratory.
In agreement with the idea that FF HDL participate in UC efflux, ABCA1 and ABCG1 transporters were detected both at the mRNA and protein levels (by immunofluorescence) in eggs (Quiroz et al., 2020). A strong signal for ABCA1 was observed at the egg surface. In addition, we also observed that ABCA1 KO eggs stained with filipin showed ∼30% higher fluorescent levels than WT eggs, suggesting that ABCA1 plays a role in mediating cholesterol efflux from eggs to HDL. Human FF HDL are mostly small and contain low UC content, biochemical features that favor the affinity of this HDL subclass with ABCA1 - and not with ABCG1 (Jaspard et al., 1996; Jaspard et al., 1997). This evidence, together with the fact that ABCG1 KO females are completely fertile (Kennedy et al., 2005), suggests a less significant function of this transporter in egg cholesterol efflux. ABCA1 KO females show approximately 80% reduction in total plasma cholesterol, and HDL with almost no cholesterol and an abnormal phospholipid composition (McNeish et al., 2000; Francone et al., 2003). These females have reduced fertility, with lower pregnancy rates and smaller litter sizes (Christiansen-Weber et al., 2000). Two independent abnormalities seem to combine in these animals. First, their placenta is malformed, so fetuses develop with severe growth retardation, and almost 50% of pups die in utero or before weaning. This altered development seems to be explained by a reduction in ovarian steroids: both estrogen and progesterone levels are ∼50% lower in pregnant ABCA1 KO when compared to WT females (Christiansen-Weber et al., 2000). Second, pregnancy rates after mating in ABCA1 KO females are around 45%, suggesting an additional defect in one or more events before pregnancy or placenta development, e.g., gamete production, fertilization, and activation. We recently reported that ∼20% of eggs retrieved from the oviducts of ABCA1 superovulated females appear dead, with a morphology reminiscent of that observed in SR-B1 KO eggs (Quiroz et al., 2020). Although characteristics of ABCA1 KO mice mentioned here suggest that the mechanism explaining the infertility is complex, our recent evidence indicates that the absence of ABCA1 in KO eggs affects their cholesterol homeostasis. Altogether, the phenotype observed in eggs from SR-B1 and ABCA1 KO mice supports the hypothesis that FF HDL and ABC transporters in oocytes are part of a cholesterol homeostatic mechanism preventing UC cholesterol accumulation in oocytes that may be relevant for fertility.
The role of UC as a pathogenic player in female mouse infertility was also suggested by evidence from two genetically modified mouse models with altered HDL metabolism. In one of these models, the PDZ domain containing 1 (PDZK1) KO mouse, a normal UC:TC proportion despite abnormal HDL levels does not affect fertility in females (Kocher et al., 2003). PDZK1 is a multi-PDZ domain containing adaptor protein that interacts with SR-B1 and stabilizes this receptor in a tissue-specific and post-translational manner (Trigatti, 2017). The expression of PDZK1 in the liver is necessary for the stability of SR-B1 in this organ. PDZK1 deficient mice show normal SR-B1 content in ovaries and other steroidogenic tissues, but a very low hepatic SR-B1 levels (5% vs. WT controls) (Kocher et al., 2003). Like SR-B1 KO animals, PDZK1 KO mice have abnormally large, cholesterol-enriched circulating HDL particles. However, the UC:TC ratio in these particles remains almost normal and female PDZK1 KO mice are fertile. An opposite situation is observed in SR-B1 KO mice transgenically expressing Human Cholesteryl Esther Transfer Protein (CETP): TgCETP SR-B1 KO (Hildebrand et al., 2010). CETP is a hydrophobic plasma glycoprotein mainly expressed in human liver, spleen and adipose tissue and naturally absent in mice (Agellon et al., 1991). This enzyme mediates the mobilization of CE between HDL and other lipoproteins, such as VLDL and LDL, so these larger lipoproteins carry around ∼65% of plasma cholesterol in humans (Tall, 1993). Mice and rats do not express functional CETP and cannot transfer CE from HDL to other lipoproteins, so almost all plasma cholesterol in rodents is contained in HDL. TgCETP SR-B1 KO mice were generated to study if CETP-mediated transfer of CE from HDL to larger lipoproteins could replace the inefficient reverse cholesterol transport observed in SR-B1 KO and serve as a compensatory mechanism to prevent atherosclerosis. CETP expression in SR-B1 KO mice significantly reduced HDL particle size and TC levels; however, the UC:TC ratio remained high compared to that in WT females, and TgCETP SR-B1 KO were unable to generate litters over a period of 6 months.
As described previously, UC may become esterified by LCAT within lipoproteins. In LCAT KO mice, plasma esterified cholesterol is significantly reduced whereas plasma UC level remains essentially unchanged, so despite a very high UC:CE ratio, UC levels are normal (Ng et al., 1997). The fact that LCAT KO females are fertile shows that not only the percentage of UC but the net amount of this toxic lipid in lipoproteins is relevant for fertility.
Conclusion
Cholesterol has been traditionally considered essential for female fertility mainly based on its relevance as a substrate for steroid synthesis in ovarian follicular cells. However, recent work provided evidence showing that oocyte cholesterol homeostasis may also be relevant ensure the developmental potential of eggs.
The small size and low cholesterol content in FF HDL, which apparently permeate from circulation and subsequently undergo remodeling inside the follicle, suggest that these particles play an essential role in maintaining oocyte cholesterol levels within appropriate ranges by participating as acceptors for UC. Indeed, females from mouse models with HDL containing excess UC or undergoing impaired reverse cholesterol transport are infertile. In some of these animals, UC accumulation in oocytes is associated with an increased lability and reduced capacity of generating live embryos.
Infertility affects around 1 in 10 young couples, and as far as 30% of female infertility is idiopathic (of unknown cause) (Sadeghi, 2015). Disorders of lipid metabolism, particularly classic dyslipidemia characterized by high triglycerides and a low HDL-to-LDL cholesterol ratio, are considered independent risk factors of lower fecundability in women (Pugh et al., 2017; Pirnat et al., 2018; Jamro et al., 2019). However, the participation of abnormal HDL in the etiology of infertility in women remains largely unexplored. As far as we are aware of, only one study demonstrated that infertility is associated with disruptions in circulating UC levels. In the Longitudinal Investigation of Fertility and Environment (LIFE) study, higher serum UC levels were reported in couples who did not become pregnant during the 12-months follow-up period. This association was also observed when female partners were analyzed independently: high UC levels were observed in women showing longer time to pregnancy as an outcome of fertility (Schisterman et al., 2014). Unfortunately, neither the distribution of UC among different lipoproteins nor the oocyte phenotype were reported in that study.
Our current working model (Figure 1) proposes that FF HDL are part of a homeostatic mechanism that ensures adequate cholesterol levels in developing oocytes and eggs by removing excess cholesterol from the PM. Deficient reverse cholesterol transport by abnormal FF HDL results in UC excess in the egg, an alteration that can interfere with biological events modulating oocyte maturation, meiosis arrest and egg activation after fertilization. Thus, alterations in HDL cholesterol metabolism may impact FF HDL function and result in infertility due to egg dysfunction and lability.
FIGURE 1. Working model. We propose a homeostatic mechanism in the ovary in which FF HDL ensure adequate cholesterol levels in the egg by removing excess cholesterol from the oocyte during the antral stage of follicular development. SR-B1: Scavenger Receptor Class B Type 1; ABCA1: ATP-Binding Cassette transporter A1; CETP: Cholesteryl Esther Transfer Protein; LCAT: Lecithin Cholesterol Acyl Transferase; PDZK1: PDZ domain containing 1. Created with BioRender.com.
Author Contributions
AA: Writing introductory section and section UC and oocyte biology, review final draft; AQ: writing section FF HDL and oocyte developmental potential, review final draft; NS: Writing section lipoproteins and steroidogenesis, preparing Table 1 and Figure 1, review final draft; EM: writing UC and induction of cell death mechanisms, review final draft; DB: Conceptualization, organization and structuring of review, writing section mouse models of abnormal HDL metabolism and infertility, reviewing final draft, preparing Figure 1, submitting, funding acquisition.
Funding
This manuscript was supported by the Chilean National Agency of Research and Development [Agencia Nacional de Investigación y Desarrollo (ANID)] programs: BASAL #FB210024 and National Fund for Scientific and Technological Development [Fondo Nacional del Desarrollo Científico y Tecnológico (FONDECYT)] #1221376 (to DB) and #1200499 (to EM), and Fellowships #21211964 (to AA) and #21170306 (to AQ). NS in funded by institutional funds from Universidad de O'Higgins.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Acknowledgments
The authors would like to acknowledge Prof. Monty Krieger (Department of Biology, Massachusetts Institute of Technology, Cambridge, United States) and Dr. Attilio Rigotti (Department of Nutrition, Diabetes and Metabolism, School of Medicine, Pontificia Universidad Católica de Chile), and Drs. Omar Francone (Homology Medicines, Inc., Bedford, MA, US) and Silvana Zanlungo (Department of Gastroenterology, School of Medicine, Pontificia Universidad Católica de Chile) for supporting our initial studies and donating the SR-B1 KO and ABCA1 KO mouse lines, respectively.
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Keywords: female fertility, high-density lipoprotein metabolism, cholesterol, unesterified cholesterol, oocyte
Citation: Arias A, Quiroz A, Santander N, Morselli E and Busso D (2022) Implications of High-Density Cholesterol Metabolism for Oocyte Biology and Female Fertility. Front. Cell Dev. Biol. 10:941539. doi: 10.3389/fcell.2022.941539
Received: 11 May 2022; Accepted: 01 June 2022;
Published: 14 September 2022.
Edited by:
Nicolas Brukman, Technion Israel Institute of Technology, IsraelReviewed by:
Pablo Daniel Cetica, Universidad de Buenos Aires, ArgentinaAhmed Ziyyat, Université de Paris, France
Copyright © 2022 Arias, Quiroz, Santander, Morselli and Busso. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Dolores Busso, dbusso@uandes.cl