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Front. Cell Dev. Biol., 29 June 2022
Sec. Cell Growth and Division
This article is part of the Research Topic Editors' Showcase 2021: Insights in Cell Growth and Division View all 11 articles

Embryonic Programs in Cancer and Metastasis—Insights From the Mammary Gland

  • Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore
GRAPHICAL ABSTRACT
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GRAPHICAL ABSTRACT. Shared molecular programs in embryonic mammary gland cells and breast cancers. Key developmental pathways are often reactivated or dysregulated during tumorigenesis and metastagenesis. The identification of embryonic signatures in tumors and metastasis could pinpoint novel prognostic markers and targets for therapeutic intervention. Figure created with BioRender.

Introduction

The mammary gland is the definitive feature of species in the class of Mammalia. Development of the mammary glands starts in the mouse embryo at embryonic (E) day 10.5 with the specification of the mammary line. By E11.75, all five mammary rudiment (MR) pairs in the mouse are present as disk-shaped, multi-layered placodal structures that will grow and acquire, sequentially, a morphology characterized by hillock, bud and bulb. Conventionally, the MR pairs are numbered 1 to 5 by their position along the antero-posterior axis. At E14.5, MR development diverges between the two sexes in a process called sexual dimorphism. MR development halts in males but proceeds in females with bulb enlargement and its recession into the mesenchyme. At E18.5, just before birth, the MR consists of a rudimentary ductal tree structure of 10–15 branches embedded within the mammary fat pad (Veltmaat et al., 2003; Spina and Cowin, 2021). The MRs develop asynchronously in the order of MR3, MR4, MR1 and MR5 and finally MR2, as determined by histological examination (Lee et al., 2011).

As a skin appendage, the murine embryonic mammary glands are excellent models for understanding developmental processes such as ectodermal specification, epithelial-mesenchymal cross-talks, morphogenesis, and their underlying cellular and molecular mechanisms. Various spontaneous mouse mutants and genetically engineered mouse models (GEMMs) have greatly facilitated the discovery of genes and signaling pathways that regulate mammary gland development (Veltmaat, 2017). This deeper understanding of developmental mechanisms has also proffered new perspectives regarding pathological conditions such as cancer. Indeed, the phases in embryonic mammary gland development resemble the phases of tumorigenesis and cancer progression. For example, the induction of the embryonic MR at around E11.5 and subsequent growth mediated by ectodermal cell recruitment (Lee et al., 2011) could be likened to cellular transformation and the development of carcinoma in situ. Further, MR sprouting at E15.5 and branching morphogenesis at E18.5 resemble the invasion of the basement membrane and tumor stroma during the metastatic cascade. Intriguingly, these embryonic morphogenetic events that mimic stages of tumorigenesis and metastasis also share similarities at the molecular level. Collectively, insight into these associations raises the possibility that cancer cells may leverage upon early developmental pathways and molecular programs to spur pathogenic development. Reversion to a more undifferentiated, embryonic-like state may promote processes that are associated with malignancy such as proliferation, epithelial-to-mesenchymal transition (EMT), cancer stem cell (CSC) formation, invasion, and metastasis (Kelleher et al., 2006; Takebe et al., 2011; Lee et al., 2019) (Figure 1A).

FIGURE 1
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FIGURE 1. Modes by which the embryonic mammary gland may contribute to breast cancer development. (A) Reactivation of embryonic mammary gland genes or signaling pathways may promote cancer development. (B) Exposure to carcinogens may remodel the postnatal mammary gland and increase breast cancer risk. (C) Embryonic mammary gland cells may contribute to the stem cell pool in the postnatal mammary gland which may be cells of origin of cancer. Note: a representative MR is depicted, however, each mode could be plausibly applied to MRs in other stages of development. Figure created with BioRender.

Does embryonic developmental history affect postnatal development and susceptibility to cancer? Development of the MRs during embryogenesis may lay the foundation for growth and morphogenesis during the postnatal phase, which is subsequently mirrored during neoplastic transformation. It has been observed that the postnatal thoracic mammary glands (MGs 1, 2, and 3) tend to form mammary tumors more frequently than inguinal glands (MGs 4 and 5) (Sheldon et al., 1982; Minasian, 1983; Bolander, 1990). This has been attributed to the presence of more epithelial tissue in the thoracic glands compared to the inguinal glands that can undergo neoplastic transformation (Vaage, 1984). Moreover, the asynchronous differentiation between the thoracic and inguinal mammary glands results in increased less-differentiated structures such as the terminal end buds in the thoracic glands. This may explain the thoracic glands’ increased susceptibility to 7,12-dimethylbenz(a)anthracene (DMBA)-induced carcinogenesis (Russo and Russo, 1988). These observations are in line with studies showing that the mouse mammary tumor virus long terminal repeat-driven Polyoma virus middle T antigen (MMTV- PyMT) and MMTV-cNeu mouse models generate more tumors in specific thoracic mammary glands than inguinal ones (Veltmaat et al., 2013) (Figure 1B). Lastly, embryonic mammary gland development is adversely affected by its exposure to endogenous and synthetic estrogens such as Bisphenol-A (BPA) which is linked to increased breast cancer risk during adulthood (Acevedo et al., 2013; Speroni et al., 2017) (Figure 1B). Taken together, physiological differences that are associated with differential tumorigenic potential could, in part, be attributed to differential molecular regulation that has taken place in embryonic development.

The approach of seeking to understand pathological conditions from the study of normal development has provided new perspectives into the origins of cancer. Some suggest that breast cancer may have a stem cell origin as the transcription factors that normally regulate gene expression in embryonic stem or progenitor cells are also misregulated in breast cancers (Briegel, 2006). Moreover, lineage tracing studies show that proliferating, long label-retaining embryonic cells (Boras-Granic et al., 2014) and a Lgr5+Tspan8hi subpopulation (Fu et al., 2017) may contribute to the population of long-lived, quiescent mammary stem cells, which may be precursor cancer cells in the adult (Figure 1C). These findings imply the potential to simplify and deconvolute the study of tumors and their significant cellular and molecular heterogeneity by considering and focusing on subpopulations of cells having embryonic origins or molecular signatures.

This review provides a comprehensive summary of all known genes and signaling pathways that lead to aberrant embryonic mammary gland phenotypes in GEMMs as well as breast cancers and metastasis when dysregulated. Following this, recent studies that compare molecular signatures of the embryonic mammary glands and breast cancers are summarized. Evidence is provided to support the proposal that studying the development of the mammary glands aligns with providing an understanding of the mechanisms of cancer, with the intention to identify novel prognostic markers and therapeutic strategies against this prominent disease.

Signaling Pathways in Embryonic Mammary Gland Development and Cancer

WNT Signaling

WNT signaling regulates numerous developmental processes. The name “WNT” is a combination of wingless and integration site-1 (Int1), of which the latter was identified as a genetic locus activated by the insertion of the MMTV that leads to the formation of mammary tumors (Nusse and Varmus, 1982). Presently, there are 19 known WNT ligands in mammals. Classical WNTs (WNT1, WNT3A, WNT8, and WNT8B) activate the canonical β-catenin pathway while non-classical WNTs (WNT4, WNT5A, and WNT11) activate the non-canonical WNT/calcium pathway.

The canonical WNT/β-catenin pathway primarily acts to regulate cytosolic β-catenin levels (Figure 2). Without WNT, β-catenin is targeted to the APC/AXIN destruction complex where it is phosphorylated by CK1 and GSK3β (Kimelman and Xu, 2006). Consequently, phosphorylated β-catenin is ubiquitinated by the E3 ubiquitin ligase β-TrCP and targeted for proteasomal degradation. In the absence of WNT, LEF/TCF transcription factors bind to WNT response elements, enabling the recruitment of co-repressors such as Groucho and histone deacetylases (HDACs) to promote chromatin compaction and inhibit target gene transcription. In the presence of WNT however, DVL recruitment by the frizzled receptor (FZD) results in the phosphorylation of co-receptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP5/6) and AXIN recruitment. This inhibits the AXIN-mediated phosphorylation and degradation of β-catenin, resulting in its accumulation and nuclear import. Nuclear β-catenin binds to LEF/TCF, replaces transcriptional repressors and recruits members of the switch/sucrose non-fermentable (SWI/SNF) family of transcriptional coactivators and other chromatin remodellers. Transcriptional co-activators BCL9/LGL and pygopus (PYGO) aid in the transport of these proteins to the TCF/β-catenin complex. As the chromatin becomes less compacted and consequently more accessible, the transcription of WNT-target genes will proceed (Nusse and Clevers, 2017) (Figure 2).

FIGURE 2
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FIGURE 2. Canonical WNT/β-catenin signaling in the embryonic mammary gland. In the absence of WNT (left, OFF state), cytoplasmic β-catenin is targeted to the destruction complex comprising AXIN, APC, GSK3β and CK1 where it is phosphorylated. Phosphorylated β-catenin is ubiquitinated by the E3 ubiquitin ligase β-TrCP, which targets β-catenin for proteasomal degradation. WNT target genes are repressed by Groucho and histone deacetylases (HDACs). LRP4 and its potential ligand, WISE/SOSTDC1 inhibits WNT signaling. In the presence of WNT ligand (right, ON state), a receptor complex forms between FZD and LRP5/6. DVL recruitment by FZD leads to LRP5/6 phosphorylation and AXIN recruitment. Consequently, degradation of β-catenin is disrupted, allowing β-catenin to accumulate in the nucleus where it functions as a co-activator of LEF1/TCF to promote the transcription of target genes. Genes highlighted in red denote those that give rise to aberrant embryonic mammary gland phenotypes when deleted or overexpressed. P denotes phosphorylation events after overexpressed. Figure created with BioRender.

WNT signaling plays critical roles in embryonic mammary gland development as inhibition and dysregulation of multiple pathway components results in aberrant phenotypes (Table 1, Figure 2). The absolute requirement for WNT signaling is demonstrated by the failure of MR induction caused by the expression of the secreted WNT inhibitor, Dickkopf-1 (Dkk1) under the ectoderm- and epithelium-specific Krt5 promoter (Chu, Hens et al., 2004). In Lef1−/− embryos all MRs regress by E15.5, with MR2 and MR3 being the first to regress shortly after induction, highlighting the requirement of WNT signaling for MR maintenance (van Genderen et al., 1994; Boras-Granic et al., 2006). Lrp5−/− and Lrp6−/− embryos form hypoplastic MRs. Additionally, Lrp6−/− embryos display defects in branching morphogenesis (Lindvall et al., 2006; Lindvall et al., 2009). Lrp4mdig/mdig (Lrp4 hypomorph) embryos display a delay in MR initiation as well as an aberrant number and distribution of mammary precursor cells leading to abnormal morphology, number and positioning of the MRs. In contrast to the previous WNT signaling-associated mutants, the Lrp4mdig/mdig mammary defects are associated with abnormally elevated WNT/β-catenin signaling. In support of this, Lrp4mdig/mdig mammary defects are abrogated by heterozygous or homozygous-null alleles of Lrp5 and Lrp6, as well as the deletion of ectoderm and MR-specific β-catenin with the Krt14-Cre promoter (Krt14-Cre;β-cateninflox/flox) (Ahn et al., 2013). Lrp4 interacts with Wise/Sostdc1 which modulates WNT signaling and inhibits bone morphogenetic protein (BMP) signaling (Itasaki et al., 2003; Laurikkala et al., 2003; Lintern et al., 2009). Wise−/− mice phenocopies a subset of the Lrp4 mammary defects, including elevated WNT/β-catenin signaling whereas Wise overexpression reduces the number of mammary precursor cells. Finally, embryos null for Pygo2 or lack ectoderm- and mammary epithelium-specific Pygo2 (Krt14-Cre;Pygo2flox/flox) show aberrant induction, sprouting and branching, most often in the thoracic MRs (Gu et al., 2009). Taken together, the levels of WNT/β-catenin signaling must be precisely regulated for proper MR development.

TABLE 1
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TABLE 1. Genes and signaling pathways critical for embryonic mammary gland development, their corresponding role in breast cancer, involvement in other cancers, and available therapeutic targets and strategies.

Despite there being a lack of mutations to pathway genes, an upregulation in WNT activity has been detected in most breast cancers and is linked to reduced overall survival (Zhan et al., 2017). High levels of β-catenin and its target gene, CCND1 were detected in 60% of breast cancers and correlates with poor prognosis (Lin et al., 2000). Decreased expression of the WNT inhibitory factor (WIF1) (Ai et al., 2006) and an elevated expression of WNT ligands are commonly observed in breast cancer (Xu et al., 2020). Aberrant epigenetic changes, including methylation of the APC gene promoter which could dysregulate WNT signaling has also been detected in inflammatory breast cancer (Van der Auwera et al., 2008). Importantly, WNT hyperactivity may result due to other cancer-associated mutations and aberrant activation of cancer-associated signaling pathways. For example, stabilization of β-catenin by WNT-independent pathways, such as PIN1, P53, PTEN/AKT, and NF-κB pathways, plays a significant role in breast cancer and malignant progression (Incassati et al., 2010). Intriguingly, the embryonic transcription cofactor limb-bud and heart (LBH), a direct target of WNT signalling in epithelial development, is overexpressed in the basal subtype of breast cancer (Rieger et al., 2010); this is suggestive of a WNT-mediated reversion to an embryonic-like state during tumorigenesis.

WNT signaling activation is implicated in metastasis. Cytokine signaling from the local bone microenvironment activates NF-κB and cAMP response element-binding protein (CREB) signaling in breast cancer cells, which in turn, initiates an autocrine WNT signaling loop. This leads to CSC colony formation in the bone marrow (Eyre et al., 2019). Importantly, inhibition of WNT signaling by recombinant human DKK1 or anti-FZD is sufficient to prevent metastatic colonisation in the bone. Other therapeutic strategies to inhibit WNT signaling include the use of small molecule inhibitors against the FZD receptor, destruction complex, nuclear β-catenin, and WNT ligand modifying enzymes such as Porcupine and Tankyrase (Wen et al., 2020).

Hedgehog Signaling

The Hedgehog (HH) pathway is activated by HH ligand binding to patched (PTCH), a transmembrane transporter-like protein located at the cilium. Upon HH binding, the suppression of PTCH on the seven-span transmembrane protein smoothened (SMO) is released, leading to the release of SMO-mediated inhibition on the suppressor of fused (SUFU) (Wang et al., 2007). As a result, phosphorylation and proteolytic processing of the GLI family of zinc-finger transcription factors are inhibited and full-length proteins that function as transcriptional activators accumulate (Kise et al., 2009; Humke et al., 2010). GLI1, GLI2, and GLI3 mediate HH signaling in vertebrates; whereas GLI1 is exclusively a transcriptional activator, GLI2 and GLI3 could either activate or repress transcription (Figure 3A).

FIGURE 3
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FIGURE 3. Signaling pathways in the embryonic mammary gland. Cartoon depicting major signaling pathways that are critical during embryonic mammary gland development. (A) Hedgehog signaling. Although Hh ligands are expressed in the MRs, Hedgehog signaling is in the inactivated state. GLI is maintained in the repressor form to repress the transcription of Hh target genes. (B) FGF signaling. FGF10 binding to its main receptor, FGFR2B triggers receptor dimerization, phosphorylation and the activation of diverse downstream pathways. (C) IGF1R signaling. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosine phosphorylation of multiple signaling adapter proteins including, the insulin receptor substrates (IRS1/2). (D) PTHRP signaling. PTHRP binding to the G-coupled receptor PTHR1 activates AC and PLC downstream signaling. (E) BMP signaling. Binding of ligand to the receptor complex stimulates BMPR autophosphorylation and phosphorylation of downstream substrates. BMP4 may interact with PTHRP signaling to facilitate epithelial-mesenchymal cross talk. (F) EDA-EDAR signaling. EDA binding to EDAR triggers downstream NF-kB signaling. (G) NRG3-ERBB4 signaling. NRG binding triggers receptor dimerization and activation of receptor tyrosine kinase domain and downstream signaling. (H) NOTCH signaling. Ligand binding triggers the cleavage of N1ICD which activates downstream signaling. (I) Hormone signaling. Binding of hormones such as estrogen or progesterone to their cognate hormone receptor (HR) promotes internalization of the hormone-receptor complex. Homo- or heterodimer formation ensues followed by translocation into the nucleus and binding to DNA response elements and transcription or repression of target genes. Only relevant components of each signaling pathway in embryonic mammary gland development are depicted. Genes highlighted in red denote genes that give rise to aberrant embryonic mammary gland phenotypes when deleted or overexpressed. P denotes phosphorylation events. Figure created with BioRender.

HH ligands namely Sonic hedgehog (Shh) and Indian hedgehog (Ihh) are expressed in the MRs at E12.5. Despite this, Shh−/− embryos induce all MRs and show normal branching morphogenesis at E16.5 (Michno et al., 2003). Similarly, transplantation of Shh−/− and Ihh−/− MRs into wildtype cleared fat pads results in normal branching morphogenesis. Taken together, these observations indicate that epithelial Shh and Ihh are dispensable for MR induction and branching morphogenesis (Gallego et al., 2002).

Gli1 is a reliable marker of HH signaling activation as Gli1 is a direct transcriptional target of HH signaling and its expression is strictly dependent on HH signaling pathway activation transduced by either Gli2 or Gli3 transcriptional activators (Dai et al., 1999). While Gli1 expression is not detected in the somites underlying MR3 at E11.5, Gli2 and more prominently, Gli3, are expressed. Gli1 expression is absent in the MRs from E11.5 to E14.5. This indicates that HH signaling is inactive in the somites and MRs in these stages (Hatsell and Cowin, 2006). The upregulation of Gli1 and, to a lesser extent, Gli2 in Gli3Xt-J/Xt-J (Gli3 null) MRs indicates that Gli1 expression is suppressed by Gli3 repressor during normal MR development. Furthermore, the loss of Gli3Xt-J/Xt-J MR3 and MR5 is phenocopied by the expression of Gli1 under the Gli2 promoter and Gli3 heterozygosity (Gli21nki/1nki; Gli3Xt/+). Altogether, this shows that HH signaling must be inactivated for MR3 and MR5 induction (Hatsell and Cowin, 2006; Lee et al., 2013). Moreover, aberrant activation of HH signaling in Gli3Xt-J/Xt-J embryos results in hypoplasia as well as defective bud and branching morphogenesis in MR2, MR4, and to a lesser extent, MR1 (Lee et al., 2011). Gli1lzki/lzki (Gli1 null) and Gli2lzki/lzki (Gli2 null) embryos induce all MRs normally (Hatsell and Cowin, 2006).

In contrast to embryonic mammary gland development, accumulating evidence suggests that HH signaling is activated during tumorigenesis. Conditional over-expression of GLI1 with the MMTV promoter expands mammary progenitor cells, upregulates genes involved in proliferation, cell survival, EMT and metastasis and results in tumors that display the malignant basal or hybrid basal and luminal epithelial phenotypes (Fiaschi et al., 2009). Noteworthy, HH signaling components and other oncogenic pathways integrate to synergistically promote breast tumorigenesis. Specifically, GLIs may be modulated by non-HH signaling pathways through the integration of signals from TGFβ (Javelaud et al., 2011) and NF-κB (Colavito et al., 2014) signaling pathways. Similarly, although Gli3 does not have a direct role in breast cancer, it has been found to cooperate with other genes such as androgen receptor (AR) (Lin et al., 2022), estrogen receptor (ER) (Massah et al., 2021) and Eph10A (Peng and Zhang, 2021) to promote tumorigenesis and invasive phenotypes. In silico analyses of gene expression profile datasets identified GLI3 as a putative interacting partner of TBX3, an important regulator in embryonic mammary gland development commonly overexpressed in breast cancer (see also section on TBX below). Further sequence-based and chromatin immunoprecipitation analyses show that Gli3 is a direct transcriptional target of TBX3 (Mosca et al., 2009).

The HH signaling pathway is also activated in the CSC-enriched CD44+CD24−/low population and side population of the MCF7 breast cancer cell line (Tanaka et al., 2009). The interaction of PTCH1 with the membrane glycoprotein, TSPAN8, leads to enhanced SHH signaling, increased tumor formation in mice and resistance to chemotherapeutic agents (Zhu et al., 2019). These observations suggest a link between the deregulation of HH signaling and the acquisition of cancer stemness and therapeutic resistance. Finally, in metastasis, paracrine signaling and activation of the HH pathway in stromal cells via tumor cell HH ligand overexpression increases invasiveness and metastasis in breast cancer (O'Toole et al., 2011).

The HH pathway has been extensively explored as therapeutic targets for breast cancer (Bhateja et al., 2019). Treatment of cyclopamine, a naturally-occurring steroidal alkaloid that inhibits the HH pathway by binding to SMO, suppresses Gli1 expression and the growth of HH pathway-activated breast carcinoma cells (Kubo et al., 2004). GLI inhibitors have also been extensively developed for clinical trials (Bhateja et al., 2019; Riobo-Del Galdo et al., 2019).

Fibroblast Growth Factor Signaling

The fibroblast growth factor (FGF) family is composed of at least 22 members and 4 FGF receptors (FGFRs) that are involved in various aspects of vertebrate development (Pownall and Isaacs, 2010). Upon ligand binding, FGFRs dimerize and become phosphorylated in the intracellular tyrosine kinase domains, which, in turn, leads to the activation of various downstream proteins; e.g., FGFR substrate 2 (FRS2), phospholipase Cγ (PLCγ), among others. FGF signals are typically transduced by the RAS/MAPK, PI3K/AKT, or PLCγ downstream cascades which regulate a myriad of cellular processes like cell growth. Given its wide-ranging effects, FGFR signaling is tightly regulated, exemplified by its negative regulation by MAPK phosphatase 3, Sprouty proteins, and similar expression to FGF (SeF) family members (Santolla and Maggiolini, 2020) (Figure 3B).

FGF10 and its main receptor, FGFR2B are involved in epithelial-mesenchymal interactions, as suggested by their complementary expression domains in the MRs. At E10.5, Fgf10 is expressed in the ventral region of the thoracic somites (Mailleux et al., 2002; Veltmaat et al., 2006). From E11 to E12, Fgfr2b is expressed in the mammary epithelium (Spencer-Dene et al., 2001; Mailleux et al., 2002). Strikingly, Fgf10−/− embryos only form MR4 (Mailleux et al., 2002; Veltmaat et al., 2006). Even so, development of MR4 proceeds aberrantly as at E18.5, Fgf10−/− MR4 consists only of a sprout connected to the nipple, demonstrating the involvement of Fgf10 in branching morphogenesis in addition to MR induction. The requirement of FGF signaling in branching morphogenesis is consistent with the finding that at E18.5, all 4 FGFRs (most prominently FGFR2 and FGFR3) are expressed in the MRs (Spike et al., 2012). Like Fgf10−/− embryos, Fgfr2b−/− embryos only induce MR4 (Veltmaat et al., 2006), albeit transiently as it regresses by E13 due to mammary epithelial apoptosis. Intriguingly, high levels of FGF10 are required for MR3 induction as Fgf10mlcv/- (Fgf10 hypomorph) embryos lack MR3 (Veltmaat et al., 2006). This suggests MR-specific requirement for FGF signaling activation.

Fgf7, another ligand of Fgfr2b, is expressed at E12.5 in the mesenchyme, before the formation of the mammary mesenchyme. This suggests that FGF7 may act redundantly with FGF10 to activate FGFR2B for MR4 maintenance. By E15.5, Fgf7 expression decreases but expands into the adjacent fat pad precursor (Mailleux et al., 2002). Other ligands of FGFR2B namely Fgf1 and Fgf3 are not expressed in the MRs.

FGF signaling misregulation is common across all breast cancer subtypes (Santolla and Maggiolini, 2020). FGFR1 amplification is the most frequent genomic alteration whereas FGFR2-4 amplification is relatively less common in breast cancer (Reis-Filho 2006). Interestingly, FGFR1 amplification may be breast cancer subtype-specific: in particular, the FGFR1 locus (8q12) is amplified in nearly 15% of hormone receptor-positive breast cancers but only in 5% of triple-negative breast cancers (TNBC). Aberrant activation of FGF/FGFR signaling caused by FGF overexpression or FGFR1 amplification and overexpression in ER+ breast cancer cells is associated with estrogen-independent cell proliferation, metastasis and reduced distant metastasis-free survival (McLeskey et al., 1998; Turner and Grose, 2010). These studies, and many others, strongly suggest the involvement of FGF signaling in malignant progression. As such, various therapeutic approaches have been developed to inhibit the pathway including small molecule inhibitors and monoclonal antibodies (mAbs) that block FGFRs or entrap FGFs (Santolla and Maggiolini, 2020).

P190b RhoGTPase Activating Protein, Insulin Receptor Substrate, and Insulin-Like Growth Factor 1 Receptor Signaling

P190b is a member of the RhoGAP family which are negative regulators of RhoGTPase activity (Burbelo et al., 1995). At E12.5, p190b is expressed in the mammary epithelium while at E14.5 p190b expands its expression domain, but at a lower level, to the adjacent mesenchyme (Heckman et al., 2007). P190b−/− embryos develop hypoplastic MRs with disorganized mammary mesenchyme that lack AR expression (Heckman et al., 2007). Thus, p190b is required for MR growth, mammary mesenchyme specification, maturation and potentially sexual dimorphism. At E14.5, p190b−/− MRs show a decrease in the expression of adaptor proteins, insulin receptor substrate 1 (IRS1) and IRS2 and the insulin-like growth factor 1 receptor (IGF1R) signaling marker, phosphorylated Akt. IRS1 and IRS2 are expressed in the mammary epithelium and mesenchyme at E14.5. Similar to p190b−/− embryos, Irs1−/−;Irs2−/− compound mutants develop hypoplastic MRs and lack mammary mesenchyme differentiation at E14.5 (Heckman et al., 2007). Finally, and consistent with the previous mutants, Igf1r−/− embryos develop hypoplastic MRs at E14.5. Taken together, P190B, IRS1, IRS2, and IGF1R form a signaling network that regulates various aspects of embryonic mammary development (Figure 3C).

Although RhoGTPases are commonly overexpressed and hyperactivated in breast cancers (Tang et al., 2008), paradoxically, P190b has been implicated as an oncogene in breast carcinogenesis. P190b haploinsufficiency inhibits MMTV-Neu tumor formation, progression, angiogenesis, and metastasis (Heckman-Stoddard et al., 2009). Consistently, specific overexpression of p190b in the mammary epithelium, also in MMTV-Neu mice, led to enhanced tumorigenesis and metastasis mediated by downstream Rac1-dependent reactive oxygen species (ROS) production (McHenry et al., 2010).

Despite their homology, IRS1 and IRS2, have distinct functions in regulating breast cancer progression (Gibson et al., 2007). Specifically, IRS2 is a positive regulator of metastasis (Nagle et al., 2004), whereas IRS1 is a suppressor of metastasis in the MMTV-PyMT mouse model (Ma et al., 2006). More recently, IRS1 has also been implicated in progesterone receptor (PR)-driven stemness and endocrine resistance in ER+ breast cancer (Dwyer et al., 2021). The IGF1R-IRS1/2 signaling axes may be important in breast cancers as at least 50% of breast tumors have activated IGF1R (Ekyalongo and Yee, 2017).

Several strategies have been developed to inhibit IGF1R signaling including the use of mAbs, small-molecule tyrosine kinase inhibitors of IGF1R and insulin receptor, and ligand neutralization. However, clinical trials show no appreciable benefit of these approaches thus far (Ekyalongo and Yee, 2017). A more promising approach uses the small-molecule tyrphostin, NT157 to target IRS; this method downregulates IRS protein expression and sensitizes ERα+ breast cancer cells to the mammalian target of rapamycin (mTOR) inhibitor, rapamycin. Moreover, NT157 inhibits the growth of tamoxifen-resistant ERα+ breast cancer cells (Yang et al., 2018).

Parathyroid Hormone-Related Protein Signaling

Parathyroid hormone-related protein (PTHRP) derives its name from and shares structural and functional similarities with parathyroid hormone (PTH). PTHrP and PTH bind to and signal through the Type 1 PTH/PTHRP receptor (PTHR1) which is a G-protein coupled receptor. Activation of PTH1R causes an acute increase in intracellular signaling molecules including the two classical G protein signaling cascades initiated by adenylate cyclase (AC) and phospholipase C (PLC). This leads to a variety of responses including the transcription of target genes and the regulation of intracellular calcium levels (Hiremath and Wysolmerski, 2013) (Figure 3D).

The complementary expression pattern of Pthrp and Pthr in the embryonic mammary gland is indicative of their roles in mediating epithelial-mesenchymal interactions. Pthrp is expressed in the mammary epithelium from the placodal stage through birth, whereas Pthr1 is expressed in the mammary and dermal mesenchyme (Wysolmerski et al., 1998). Disruption to either gene results in the induction of normal MRs, however nipple formation and subsequent development are impeded (Wysolmerski et al., 1998). Pthrp has non-cell autonomous roles as it is necessary for the differentiation and maturation of the mammary mesenchyme. In Pthrp−/− embryos, mesenchymal cells lack AR and tenascin C expression, although they continue to condense around the epithelial bud (Wysolmerski et al., 1998; Dunbar et al., 1999; Hiremath et al., 2012). The lack of AR expression causes the MRs of Pthrp−/− and Pthr1−/− male embryos to resist androgen-mediated destruction that is essential for sexual dimorphism (Dunbar et al., 1999). Conversely, overexpression of Pthrp under the control of the ectoderm- and MR-specific Krt14 promoter results in aberrant mammary mesenchyme differentiation and supernumerary nipple formation in the ventral epidermis (Foley et al., 2001). Taken together, PTHRP-PTHR1 signaling is critical for epithelial-mesenchymal cross-talks, sexual dimorphism, mesenchymal maturation, and branching morphogenesis.

Pthrp upregulates bone morphogenetic protein receptor 1a (Bmpr1a) expression in the mammary mesenchyme to enable BMP4-mediated signaling. Ductal outgrowth regulated by PTHrP may be mediated in part by BMP4 as its supplementation can rescue ductal outgrowth defects in Pthrp−/− MRs. This points to critical roles of BMP signaling in the mesenchyme and in the development of the MRs after E12.5 (Hens et al., 2007) (see section on BMP signaling). The specification of the mammary mesenchyme may also implicate the homeobox transcription factor Msx2 (Hens et al., 2007) (see also section on HOX transcription factors) and canonical WNT signaling in the mesenchyme, as its expression and activity, respectively, are dependent on Pthrp (Hiremath et al., 2012).

PTHRP and PTHR1 are often overexpressed in breast cancer and notably, PTHR1 is commonly overexpressed in breast cancer stroma (Henderson et al., 2006). Genome-wide association studies have implicated both the parathyroid hormone-like hormone (PTHLH) and the PTHR1 loci as breast cancer susceptibility genes (Garcia-Closas et al., 2013; Michailidou et al., 2013). In line with this, loss of Pthrp in the mammary epithelium of the MMTV-PyMT mice delays tumor initiation, progression, and reduces metastasis. Besides regulating genes involved in cell proliferation, angiogenesis, and apoptosis, PTHRP also affects the expression of adhesion factor CXCR4, which may be crucial for metastatic dissemination (Li et al., 2011).

PTHRP is important for metastatic colonisation in various distal sites, in particular the bone through the upregulation of osteoblastic receptor activator of NF-κB ligand (RANKL) which drives bone destruction, and downregulation of osteoprotegerin (OPG) expression (Guise et al., 2002). Consistent with this, a neutralising mAb against PTHRP diminished tumor growth and lytic bone lesions in MDA-MB-231 human breast cancer mice xenografts (Guise et al., 1996). PTHRP-specific neutralizing antibodies have also been shown to reduce lung metastases in MDA-MB-435 mice xenografts (Li et al., 2011), suggesting the broad utility of such antibodies and the feasibility of PTHRP as a target for metastasis.

Bone Morphogenetic Protein Signaling

Bone morphogenetic proteins (BMPs) constitute a large family of secreted growth factors from the transforming growth factor beta (TGFβ) family of ligands that are involved in many aspects of development. The binding of BMPs to their cognate receptors results in the phosphorylation of the “Small”, receptor-regulated “Mothers Against Decapentaplegic” homolog (SMAD) family members, notably SMAD1, SMAD5, and SMAD 8. Phosphorylated SMAD 1/5/8 forms a complex with SMAD4 that translocates into the nucleus to regulate the expression of target genes (Chen et al., 2004; Wang et al., 2014) (Figure 3E).

Bmp2 is expressed in the mammary epithelium at E13.5 (Phippard et al., 1996), although its function remains to be elucidated as Bmp2−/− mutants are not viable before the onset of mammogenesis. From E11.5 to E14.5 Bmp4 is expressed predominantly in the mesenchyme and at lower levels in the mammary epithelium (Phippard et al., 1996; Hens et al., 2007). Bmp4 is involved in various processes in the embryonic mammary gland including the positioning of the mammary line along the dorso-ventral axis, along with Tbx3, at E11.5 (Cho et al., 2006), epithelial-mesenchymal maturation and ductal outgrowth in conjunction with its receptor, Bmpr1a (Hens et al., 2007). Bmpr1a expression in the mesenchyme is regulated by PTHRP (see section on PTHRP signaling).

Aberrant expression of BMPs and misregulation of BMP signaling has been associated with breast cancer; however, their roles and effects in tumorigenesis can be context-dependent and ligand-specific. Several BMPs, including BMP2, BMP6, BMP9, BMP10, BMP15, and GDF9a inhibit the proliferation of breast cancer cells, whereas BMP4 and BMP7 can either promote or inhibit proliferation in different contexts (Alarmo and Kallioniemi, 2010; Zabkiewicz et al., 2017).

Besides proliferation, BMPs can also regulate cancer cell stemness. Recombinant BMP2 induces EMT and stemness of breast cancer cells via the Rb and CD44 signaling pathways, which leads to metastasis (Huang et al., 2017). In contrast, BMP4 may act as an autocrine mediator to activate SMAD7 and block metastasis in animal models of breast cancer. Restored BMP4 expression or therapeutically administered BMP4 protein sensitizes cancer cells to anoikis, reduces the number of circulating tumor cells and the extent of metastasis, thereby resulting in increased survival (Eckhardt et al., 2020).

Various approaches have been developed to modulate BMP signaling, including the use of BMPR kinase inhibitors and other soluble decoy receptors which can prevent the interaction of BMPs in the extracellular space with membrane-embedded receptors. Downregulation of SMAD signaling via the silencing of the E3 ubiquitin ligase, Smurf1 also attenuates BMP signaling (Lowery and Rosen, 2018).

Ectodysplasin Signaling

Ectodysplasin (Eda) is a member of the tumor necrosis factor (TNF) ligand superfamily, and functions with its receptor, Edar, to regulate the development of a variety of ectodermal appendages (Figure 3F). Upon EDA binding, EDAR recruits the adaptor protein EDARADD and via TRAF6 activates the IKK complex. This leads to the phosphorylation of I-κB, translocation of NF-κB into the nucleus and target gene transcription (Mikkola and Thesleff, 2003) (Figure 3F).

Edar is expressed in the mammary epithelium at E12 (Pispa et al., 2003) whereas Eda is expressed in the mesenchyme before and during branching morphogenesis from E15.5 to E17.5 (Voutilainen et al., 2012). Although Eda−/− (Tabby) mutants have normal numbers of mammary glands, the glands have smaller ductal trees with fewer branches (Mustonen et al., 2003). Conversely, overexpression of Eda using the Krt14 promoter induces supernumerary MRs which develop into mature glands in the adult, between MR3 and MR4 (Mustonen et al., 2003; Mustonen et al., 2004). Krt14-Eda MRs display precocious branching morphogenesis and ectopically activate NF-κB. EDA signaling is likely mediated by NF-κB as inhibition of NF-κB, concurrent with Eda overexpression result in smaller ductal trees with fewer branches at E18 (Voutilainen et al., 2012). Important regulators of embryonic mammary gland development namely PTHrP, Wnt10a, Wnt10b and other genes such as the epidermal growth factor (EGF) family ligands, Areg and Epgn, have been identified as potential transcriptional targets of EDA/NF-κB signaling during ductal development, suggesting the integration of these pathways for MR development.

Krt14-Eda mice do not show palpable tumors, however, elevated EDAR signaling in EdarTg951 (Edar copy-number amplification) transgenic mice results in a high incidence of mammary tumors in breeding female mice. These tumors may bear important, clinical-relevant characteristics as they resemble EDAR-high human tumors which lack ER expression but have elevated β-catenin transcriptional activity and extensive squamous metaplasia (Williams et al., 2022).

Neuregulin 3

The neuregulin (Nrg) family consists of four genes, Nrg1, Nrg2, Nrg3, and Nrg4, which is characterized by a conserved domain related to the EGF family of ligands. Nrg3 is a ligand for the receptor tyrosine kinase erythroblastic leukaemia viral oncogene homolog 4 (ERBB4) that belongs to the ErbB receptor tyrosine kinase family (Zhang et al., 1997). Ligand binding causes the receptor to dimerize and activate intracellular tyrosine kinase domain, leading to the activation of downstream signaling cascades such as the PI3K/AKT and MAPK pathways to regulate various processes (Stern, 2000; Hayes and Gullick, 2008) (Figure 3G).

In embryonic mammary gland development, Nrg3 is likely involved in early inductive events as it is expressed in the dermal mesenchyme adjacent to the presumptive mammary region and the presumptive mammary region itself at E11 (Howard et al., 2005). Subsequently, Nrg3 is expressed in the mammary epithelium. Like Nrg3, Erbb4 is expressed in the dermal mesenchyme underlying the presumptive MR3 and MR4 at E11.5. Subsequently, Erbb4 is expressed in the mammary epithelium and ectoderm at E12.5 and E13 (Howard et al., 2005).

Scaramanga (Nrg3ska, Nrg3 hypomorph) embryos often fail to induce MR3 but induce supernumerary MRs adjacent to the site of MR4, suggesting the anatomical region-specific roles of Nrg3 in mammogenesis. Application of recombinant NRG3 or Nrg3 overexpression using the Krt14 promoter induces MRs along the mammary line (Howard et al., 2005; Panchal et al., 2007). Nrg3ska MR3 display defects in mammary mesenchyme specification characterized by the downregulation of Lef1, ER, AR, and Pth1r expressions at E12.5 (Kogata et al., 2014).

In human breast cancer cell lines, NRG3 activates ectopically-expressed ERBB receptors (ERBB1-4). Whereas NRG3 is potentially overexpressed in breast cancer, paradoxically, recombinant NRG3 diminished the growth of human breast cancer cells in vitro. These results indicate potential dose-dependent effects of NRG3 (Hijazi et al., 1998). On the other hand, ERBB family receptor tyrosine kinases are commonly overexpressed in breast cancers, in particular, ErbB2 or HER2/neu amplification constitute a major breast cancer subtype found in 15–30% of breast cancers while ErbB4 overexpression is less common. Intriguingly, ErbB4 has context-dependent tumor suppressive and oncogenic roles (Sundvall et al., 2008). Therapeutics targeting ERBB receptors in breast cancer include the humanized anti-ErbB2 antibody trastuzumab (Herceptin) and the tyrosine kinase inhibitor, lapatinib which are efficacious and widely used in the clinic.

NOTCH Signaling

The NOTCH pathway mediates juxtacrine cellular signaling where transmembrane ligands on one cell activate transmembrane receptors on a juxtaposed cell (Hori et al., 2013; Siebel and Lendahl, 2017). Four receptors (NOTCH1–4) and five ligands—Delta-like ligand 1, 3, 4 (DLL1, 3, 4), Jagged 1 and 2 (JAG1, 2)—have been described in mammals (Hori et al., 2013) (Figure 3H).

NOTCH signaling is activated upon the binding a NOTCH ligand to its receptor, which triggers receptor cleavage by a member of the disintegrin and metalloprotease domain family (ADAM17 or ADAM10) and a presenilin-dependent γ-secretase complex. The cleaved intracellular domain of the NOTCH receptor (NICD) translocates into the nucleus where it forms a complex with the DNA-binding protein, CSL, and other transcriptional co-activators to drive Notch-target genes expression (Kopan and Ilagan, 2009; Hori et al., 2013).

Several in vivo lineage tracing studies demonstrate that at the population level, embryonic mammary gland cells are multipotent, bearing the capacity to give rise to basal and luminal cell lineages in the postnatal mammary gland (Van Keymeulen et al., 2011; Wuidart et al., 2016). However, NOTCH1 activation via the transgenic overexpression of NOTCH1 intracellular domain (N1ICD)—a ligand-independent active form of the NOTCH1 receptor—imposes a luminal ERα cell fate onto E13.5 cells (Lilja et al., 2018). This suggests that NOTCH signaling must be inactivated to maintain the multipotency of the embryonic mammary cells (see section on cellular lineages and stem cell potency).

NOTCH signaling is frequently deregulated in different breast cancer subtypes and is associated with the acquisition of therapeutic resistance (Lamy et al., 2017; Krishna et al., 2019; Nandi and Chakrabarti, 2020). Overexpression of the NOTCH1 intracellular domain with the MMTV promoter [MMTV-Notch1 (intra)] impairs mammary gland development and induces mammary tumors, suggesting the oncogenic role of NOTCH1 (Hu et al., 2006). Other studies also show NOTCH1 activation and its association with metastatic breast cancer cells (Mohammadi-Yeganeh et al., 2015). Interestingly, accumulating evidence points to the involvement of juxtacrine NOTCH signaling between tumor cells and cells that constitute the tumor microenvironment such as immune cells, cancer associated fibroblasts and endothelial cells to promote malignant progression (Meurette and Mehlen, 2018).

Gamma-secretase inhibitors (GSIs) are pan-NOTCH inhibitors that are the first and most extensively explored small molecules targeting the NOTCH pathway. GSIs reduce the levels of NICDs and several other substrate proteins, thereby inhibiting downstream signaling. Other promising approaches include anti-Notch mAbs which target the receptor-ligand binding domain or the negative regulatory region (NRR) of the NOTCH receptor, and, in turn, block intracellular NOTCH cleavage by γ-secretases and signal transduction (Lamy et al., 2017).

Homeobox Transcription Factors

Homeotic (Hox) genes encode for the prototypic homeobox transcription factors, which are known to be master regulators of developmental programs (Carroll, 1995). The role of Hox genes in regional specification is reflected in their sequential, partially overlapping expression domains along the antero-posterior body axis. This is also reflected in the relative positions of the Hox genes on the chromosome (Morgan, 1997).

Several homeobox factors are involved in MR development: Hoxc6−/− mouse embryos form hypoplastic thoracic MRs at E12.5 but their inguinal MRs are unaffected (Garcia-Gasca and Spyropoulos, 2000). The position-specific phenotype is consistent with the expression of Hoxc6 in the anterior somites underlying the thoracic MRs, and its absence in the posterior somites underlying inguinal MRs. Hoxb9 and Hoxd9 are expressed in the mammary mesenchyme at E12.5 (Chen and Capecchi, 1999), although their function, if any, in embryonic mammary gland development is unknown. Supernumerary mammary gland formation has been attributed to the ectopic expression of Hox genes (Schmidt, 1998). Taken together, Hox genes are important for MR-specific induction and growth.

The Msx genes belong to a small family of three homeobox-containing transcription factors related to the muscle segment homeobox gene, msh, in Drosophila (Davidson, 1995). Msx1 and Msx2 are expressed in the mammary epithelium at E13.5 (Phippard et al., 1996) whereas at E14.5, Msx2 is expressed in the mesenchyme (Satokata et al., 2000). Msx1−/− MRs develop normally (Phippard et al., 1996) while in Msx2−/−, MR4 development arrests at the sprout stage by E16.5. Msx1−/−;Msx2−/− compound mutants form a hypoplastic, protruded MR4 coincident with defective mammary mesenchyme at E15.5. These aberrant phenotypes are linked to the loss of Lef1 expression in the epithelium and mesenchyme (Satokata et al., 2000). Thus, Msx1 and Msx2 play non-redundant roles in MR development. Lastly, the paired-box homeobox gene 3 (Pax3) is expressed in the somites at E11.5 (Veltmaat et al., 2006). Pax3ILZ/ILZ (Pax3 null) mouse embryos do not form hypaxial buds of the somites and show delayed induction of MR3; this finding highlights the role of the somites for MR3 induction (Veltmaat et al., 2006).

Hox transcription factors play multiple roles in breast cancer including cell cycle control, apoptosis, angiogenesis and cell-cell communication (Lewis, 2000; Briegel, 2006). HOXB7 has been reported as an oncogene associated with the upregulation of bFGF expression (Care et al., 1998) and EMT induction (Wu et al., 2006). Bisphenol-A, a known endocrine-disrupting compound that increases the risk of breast cancer, induces the expression of estrogen-regulated Hoxc6 (Hussain et al., 2015) and Hoxb9 (Deb et al., 2016). Comparatively, other Hox family members like HOXA9 may exhibit tumor suppressive roles by upregulating the expression of BRCA1 in breast cancer cells. Moreover, HOXA9 downregulation is associated with elevated tumor invasion, metastasis, and patient mortality (Gilbert et al., 2010). Hox genes may engage epigenetic regulators to regulate tumorigenesis and metastasis (Sun et al., 2013).

One potential strategy to inhibit HOX function is via the use of HXR9 peptides which prevents HOX binding to PBX, a transcription co-activator common to many HOX proteins. HXR9 causes apoptosis in multiple breast cancer cell lines (Morgan et al., 2012). Overcoming the functional redundancies among the different HOX family members is one of the main challenges for HOX-based therapeutic strategies (Feng et al., 2021).

T-Box Transcription Factors

The T-box family (TBX) are transcriptional activators or repressors that are defined by a highly conserved T-box DNA binding domain (Wilson and Conlon, 2002). Tbx2 is expressed in the mammary mesenchyme at E11.5 while Tbx3 is expressed in the mammary epithelium between E11.5 and E12.5 (Chapman et al., 1996; Eblaghie et al., 2004). Tbx3 expression is restricted to the dorsal domain by ventral Bmp4 expression, which also determines the position of the mammary line (Cho et al., 2006). Tbx3tm1Pa/+ (Tbx3 heterozygous) mice form mammary placodes but maintenance of a subset of thoracic buds, nipple formation and ductal branching are impaired (Jerome-Majewska et al., 2005). Expectedly, Tbx3tm1Pa/tm1Pa (Tbx3 null) embryos have a more severe phenotype, failing to induce most MRs altogether (Davenport et al., 2003). Tbx2tm1Pa/+ (Tbx2 heterozygous) embryos form MRs normally whereas Tbx2tm1Pa/tm1Pa (Tbx2 null) embryos show inductive defects for MR2 and MR5.

Although induction is largely not affected in Tbx2+/−;Tbx3+/− compound mutants, some thoracic MRs regress by E18.5. For MRs that do progress, nipple formation and branching morphogenesis are frequently affected (Jerome-Majewska et al., 2005). In sum, Tbx genes play important roles in the induction, maintenance, nipple formation and branching morphogenesis of the MRs.

Both TBX2 and TBX3 are deregulated in breast cancers. Interestingly, TBX2 is found in a region of amplification on chromosome 17q23, which is common to about 20% of human breast cancers (Sinclair et al., 2003). TBX2 may be involved in malignant progression as its overexpression correlates with advanced tumor stages and with aggressive, hereditary BRCA1/2 breast cancers. Mechanistically, the deregulation of TBX2 or TBX3 may result in the bypass of P53-mediated senescence, growth arrest and apoptosis in breast cancers. TBX2 and TBX3 suppress Cdnk2a/p19Arf (p14Arf in human) transcription, which induces cell cycle arrest at the G1 and G2 phase by interfering with MDM2, a negative regulator of P53 (Briegel, 2006). The repression of p14Arf by TBX3 overexpression may be mediated by HDACs (Yarosh et al., 2008). Interestingly, estrogen signaling expands breast CSCs in MCF7 breast cancer cells through a paracrine FGF/FGFR/TBX3 signaling pathway, suggesting a role for Tbx3 in promoting stemness (Fillmore et al., 2010). On the other hand, Tbx2 has been shown to repress the expression of p21WAF1/CIP1, a P53 target necessary for P53-mediated growth arrest (Prince et al., 2004). TBX2 overexpression directly represses E-cadherin transcription and promotes EMT (Wang et al., 2012). Importantly, TBX2 and TBX3 may additionally play non-redundant roles in breast cancers. For example, TBX2, but not TBX3, is associated with increased metastatic potential of breast tumors through its regulation of adhesion molecules like cadherins and integrins (Rowley et al., 2004). Therapeutic strategies for TBX proteins, currently unavailable, could be directed towards their unique small repression domains (Chang et al., 2016).

GATA3

GATA3 belongs to a family of zinc finger transcription factors that bind to a consensus DNA sequence (A/T)GATA (A/G) in gene promoter regions to directly activate or repress target gene expression (Du et al., 2015).

GATA3 is expressed in the mammary epithelium and ectoderm at E12.5 (Asselin-Labat et al., 2007). Conditional deletion of Gata3 in these compartments under the Krt14 promoter (Krt14–Cre;Gata-3flox/flox) results in the lack of induction and hypoplasia of a variable subset of MRs, as assessed by Lef1 expression at E11.75 (Asselin-Labat et al., 2007).

GATA3 is one of the most frequently mutated genes in breast cancer and has context-dependent tumor suppressive or oncogenic roles. GATA3 heterozygosity in MMTV-PyMT mice expands the CD14+ c-kit−/lo and c-kit+ luminal progenitor cell population and promotes tumorigenesis; contrastingly, overexpression of GATA3 in the same mouse model promotes cellular differentiation, reduces angiogenesis and inhibits tumorigenesis (Asselin-Labat et al., 2011). In MCF7 human breast cancer cells, GATA3, through its transcription regulation of CCND1 and in association with PARP1 promotes cell proliferation and tumorigenesis by facilitating the G1 to S phase transition in the cell cycle. In vivo studies further show that GATA3 knockdown dramatically reduces tumor volume (Shan et al., 2014).

In the context of metastasis, GATA3 overexpression in LM2-4175 breast cancer cell line, an aggressive derivative of MDA-MB-231, inhibits cancer cell expansion in the lung. This is linked to GATA3-mediated downregulation of ID1/-3, KRTHB1, LY6E and RARRES3 as well as upregulation of genetic inhibitors of lung metastasis such as deleted in liver cancer 1 (DLC1) and progestagen-associated endometrial protein, PAEP (Dydensborg et al., 2009). GATA3 may also suppress metastasis via the reversal of EMT (Yan et al., 2010).

P63

The p63 gene, a homologue of the tumor suppressor p53, is highly expressed in the basal or progenitor layers of epithelial tissues. Very strikingly, p63−/− embryos fail to induce all MRs and all other ectodermal appendages (Mills et al., 1999; Yang et al., 1999).

P63 has multifaceted roles in breast cancer (Gatti et al., 2019). Notably, the overexpression of H-RasV12 or PIK3CAH1047R oncogenes in MCF10A and MCF12A normal breast cell lines downregulates the expression of ∆Np63, a p63 isoform, which leads to EMT, increased cell motility, and invasiveness. Importantly, silencing of ∆Np63 alone induces EMT and recapitulates the pro-migratory action of these oncogenes; highlighting ∆Np63 as a critical effector (Yoh et al., 2016). The invasive properties of p63 may be mediated through its target gene, membrane-type 1 membrane-anchored matrix metalloproteinase (MT1-MMP), a protease that is upregulated in p63-high basal breast cancers (Lodillinsky et al., 2016).

∆Np63 is also involved in controlling the self-renewal potential and expansion of mammary CSCs. Downregulation of p63 in MMTV-ErbB2-derived mammospheres significantly limits its self-renewal capacity in vitro and delays tumor growth in vivo. At the molecular level, ∆Np63 enhances HH signaling by directing the expression of SHH, GLI2, and PTCH1 (Memmi et al., 2015).

Hormone Signaling

The steroid hormones estrogen and progesterone are essential for the development and function of the breast, bone as well as the reproductive and cardiovascular systems. Classically, estrogen or progesterone binding to their cognate nuclear receptors leads to receptor dimerization, nuclear translocation and binding to DNA response elements to activate or inhibit target gene expression (Fuentes and Silveyra, 2019) (Figure 3I).

Hormone signaling is essential for sexual dimorphism, a process where MR development diverges between the two sexes in the embryonic mouse MRs at E14.5. In males, testosterone acts on the AR-expressing mesenchyme to constrict the mammary epithelium which halts further development (Durnberger and Kratochwil, 1980; Sakakura et al., 1982). When AR expression is absent or downregulated, MR development proceeds aberrantly (see sections on BMP, PTHRP, P190B RHOGAP, IRS, and IGF1R signaling).

Hormone receptor (ER or PR)-positive breast cancers constitute the major proportion of breast cancer subtypes. The transcriptional activity of ERα is regulated by its post-translational modifications and the action of nuclear receptor co-regulators, which may contribute to the development of breast cancer (Manavathi et al., 2013). The cross-talk of ER with other steroid receptors like PR affects tumor progression. Notably, progesterone may enhance the anti-proliferative effect of standard anti-estrogen therapy by influencing ER binding and its target gene transcription (Siersbaek et al., 2018).

ERα signaling has been implicated in metastasis. In ERα+ primary tumors, more than 80% of lymph node metastases, and 65–70% of overall distant metastases retain ERα expression. Moreover, ERα expression in tumors is also correlated with the development of bone and lung metastases (Saha Roy and Vadlamudi, 2012). Mechanistically, the bone tropism of metastatic breast cancer cells may be mediated by the interaction of ERα and the EMT transcription factor ZEB1, which have been shown to modulate ERα-mediated transcription induced by estrogen or cAMP signaling (Mohammadi Ghahhari et al., 2022). ERα knockdown in MCF7 breast cancer cells induces potent EMT and changes in the expression and activity of matrix macromolecules (Bouris et al., 2015). Functional cross-talks between estrogens and insulin/insulin-like growth factors (IIGFs)—by affecting the tumor microenvironment—may contribute to metastasis (Vella et al., 2020).

In the clinic, anti-estrogen therapy including selective ER modulators (SERMs), selective ER down-regulators (SERDs), and aromatase inhibitors (AIs) is the standard of care for patients with ERα+ breast cancers (Siersbaek et al., 2018).

Evidently, the preceding list of genes and signaling pathways have demonstrably clear, important roles in embryonic mammary gland development and often context-dependent roles in promoting breast cancer. GATA3 mutations are almost always associated with breast cancer compared to other cancers. Similarly, PTHRP signaling dysregulation is frequently implicated in breast cancer and bone metastasis. As a hormone-sensitive tissue, hormone signaling dysregulation has important implications for breast tissue. This, however does not imply that these genes and signaling pathways have exclusive roles or specific associations with breast cancer. Indeed, many are known to drive tumorigenesis in other tissues in different contexts. In the case of WNT signaling, loss-of-function mutations in APC were first implicated in the hereditary colon cancer syndrome, familial adenomatous polyposis whereas HH signaling dysregulation is predominantly known to drive basal cell carcinomas. These and examples of other cancers are listed in Table 1.

Parallels of Properties and Molecular Signatures of Embryonic Cells in Breast Cancer

Cellular Lineages and Stem Cell Potency

The cellular hierarchy and clonal dynamics of cells during pre- and postnatal mammary gland development have been greatly clarified by in vivo lineage tracing methodologies. Promoter specific-Cre models that activate the expression of a reporter gene encoding a fluorescent protein or lacZ enable the identification and facilitate the tracking of progenies from a defined parental cell (Kretzschmar and Watt, 2012).

By and large, in vivo lineage tracing studies with multiple promoter-Cre models suggest that during the initial stages of development, embryonic mammary cells are multipotent at the population level (Van Keymeulen et al., 2011; Rodilla et al., 2015; Trejo et al., 2017; Wuidart et al., 2018). Clonal analyses, where a small number of cells (<1%) is initially labelled, showed the progressive segregation of basal and luminal lineages and has revealed of unipotent luminal cells such as Notch1pos cells at E12.5 (Lilja et al., 2018). Other subsets of unipotent luminal cells include Axin2-expressing cells at E12.5 (Van Amerongen et al., 2012) and the zinc finger transcriptional repressor, Blimp1-expressing cells at E17.5 (Elias et al., 2017). During postnatal development, evidence for both unipotency and multipotency the mammary stem epithelia have been reported. While overwhelming evidence suggest the unipotency and lineage restriction of basal and luminal cells through in vivo lineage tracing (Van Keymeulen et al., 2011; de Visser et al., 2012; van Amerongen et al., 2012; Lafkas et al., 2013; Prater et al., 2014; Rodilla et al., 2015; Trejo et al., 2017; Lilja et al., 2018; Wuidart et al., 2018; Matsuo et al., 2022), rare bipotent basal cell clones expressing Krt5, Krt14, Lgr5 (Rios et al., 2014), the WNT target, Procr (Wang et al., 2015) and the NOTCH ligand Dll1-expressing cells (Chakrabarti et al., 2018) have also been observed.

During postnatal development, it is postulated that luminal and basal cell interactions are crucial to maintain lineage fidelity. Specifically, cell-cell interactions are mediated by TNF which is secreted by luminal cells and restricts basal cell multipotency. Ablation of luminal cells in the adult mammary gland reactivates the multipotency of normally unipotent basal stem cells both in vivo and in organoid models. Bulk- and single-cell transcriptomic analyses reveal that after luminal cell ablation, basal cells activate a hybrid basal and luminal cell differentiation program that is reminiscent of the genetic program that regulates multipotency during embryonic development before giving rise to luminal cells. This multipotency program is mediated by the activation of NOTCH, WNT and EGFR and downregulation of the TNF signaling pathways. Therefore, NOTCH, WNT and EGFR pathway inactivation, or TNF pathway activation were able to inhibit regeneration-induced basal cell multipotency. This demonstrates that heterotypic communication between luminal and basal cells—tightly regulated by embryonic pathways such as NOTCH and WNT—is essential to maintain lineage fidelity and stem cell potency in mammary epithelial stem cells (Centonze et al., 2020). It is tempting to speculate that disruption of normal cell-cell interactions that lead to a reversion to a multipotent embryonic cell state may be an early event in tumorigenesis. Similar to lineage ablation, reprogramming of cell states by an oncogenic stimulus such as PIK3CAH1047R results in the acquisition of multipotency of both basal and luminal cells in the postnatal mammary gland and the recapitulation of embryonic gene signatures (Wuidart et al., 2018). This, in turn, leads to the development of different breast cancer subtypes and the acquisition of tumor heterogeneity (Koren et al., 2015; Van Keymeulen et al., 2015).

Embryonic Molecular Signatures in EMT, Stem Cells and Breast Cancer Subtypes

Expression profiles analyses of various human tumor types have revealed the enrichment patterns of gene sets associated with embryonic stem cell identity. In breast cancers, this embryonic stem cell-like signature is often associated with high-grade, basal subtype ER tumors, with poor clinical prognosis (Ben-Porath et al., 2008).

Several recent studies have harnessed the advancements of multi-omics technologies to investigate the presence of embryonic signatures in breast tumor models. Bulk transcriptomic analyses show that subsets of embryonic mammary epithelial signature at E12.5 are activated in mouse Brca1−/−;p53+/- tumors and malignant human basal-like breast cancers. The signature is composed of genes that encode predominantly transcriptional regulators, notably Hox genes, cell cycle, and actin cytoskeleton components. There is also evidence that the embryonic signatures that are reactivated in cancers are subtype-specific. Embryonic gene subsets that include regulators of neuronal differentiation, transcription, and cell biosynthesis were enriched in non-basal-like tumor subtypes and repressed in basal-like tumors. Moreover, several embryonic genes showed significant upregulation in hormone receptor negative, and/or grade 3 breast cancers. Notably, the transcription factor, SOX11, a progenitor cell and lineage regulator of non-mammary cell types, is found to be highly expressed in some Brca1−/− mammary tumors. Cancer cells may also activate latent embryonic mesenchymal signatures to undergo EMT. A list of 25 genes—ATL3, B3GNT5, BCL11A, CDCA2, CHST2, CORO1C, DNM1L, DNMT3A, EPHA4, GPC2, HDGF, IGF2BP3, JMJD4, KIF20A, PROX1, PTDSS1, RPS6KA3, SLC16A13, SOX11, TCF7L2, TMEM38A, TMOD1, TRIB2, TTC9C, and UCHL1—were found in the 37-gene tumor-associated embryonic epithelial signature. This gene set could be further evaluated for their roles as putative regulators of EMT in breast cancers and potentially serve as new targets for therapeutic intervention in the future (Zvelebil et al., 2013).

There is evidence for significant molecular similarity of stem-like subpopulations of mammary cells which are enriched at E18.5 (Spike et al., 2012; Makarem et al., 2013; Trejo et al., 2017), to breast cancers. Specifically, the fetal mammary stem cell (fMaSC) signature was enriched among aggressive basal-like and Her2+ tumors. The co-expression of myoepithelial and luminal keratins as well as vimentin, which is characteristic of the fMaSC-like state, suggests that the reversion of cancer cells to an embryonic-like state resembling the fMaSC and/or fetal stroma (fSTR) compartments could be driven by partial EMT (Spike et al., 2012). On the other hand, a stem-like, quiescent, hormone-sensitive subpopulation of mammary gland cells which originate in the MRs, Lgr5+Tspan8hi, has been shown to bear molecular features of claudin-low breast tumors (Fu et al., 2017).

In a follow-up to the Spike (2012) study using single-cell transcriptomic analyses, Her2+ tumors and basal-like tumors - but not the equally proliferative luminal B tumors—were found to frequently show enrichment of fMaSC-like metabolic profiles including glycolysis, the Krebs cycle and fatty acid oxidation. (Giraddi et al., 2018). This finding suggests that the re-emergence of embryonic metabolic programs could be less associated with the enhancement of tumor cell proliferation. Rather, other processes associated with tumorigenesis such as cell state changes, cellular plasticity, and lineage specification, could be directed by such embryonic programs (Ben-Porath et al., 2008).

In silico analyses comparing bulk TCGA tumor gene expression data to the Giraddi mouse developmental trajectory show that normal, luminal A, and luminal B tumors map most closely to mature adult cells whereas Her2+ tumors map to slightly more immature cells. Basal tumors, which harbour great molecular heterogeneity, spanned the pseudotime encompassing both embryonic and adult cells along the basal trajectory (Thong et al., 2020). In the same study, gene expression analyses revealed that normal human mammary (NM) cells aligned to adult mouse cells whereas NM cells, which are conditionally-reprogramed (CR) in vitro to promote stem-like features, aligned more closely with mouse embryonic cells across the pseudotime trajectory. In contrast to NM cells, CR cells also develop hybrid cell states, characterized by the co-expression of basal and luminal lineage markers or epithelial and mesenchymal markers that are associated with aggressive cancers. Taken together, these results suggests that the acquisition of embryonic programs converts cells into a stem-like state, presenting with characteristics typical of a more developmentally immature phenotype.

There is also evidence that metastatic cells may leverage on embryonic or more generic pluripotency programs to facilitate malignant progression. Single-cell analyses show that low-burden metastatic cells harbouring basal- or stem-like characteristics may exploit embryonic programs for self-renewal and maintenance by the upregulation of pluripotency genes POU5F1 (also known as OCT4) and SOX2 as well as classical EMT markers (Lawson et al., 2015).

Embryonic Molecular Signatures and Their Clinical Relevance for Breast Cancer

Breast cancer and metastasis remain challenging problems globally. The striking similarities between embryonic progenitor cells to breast cancers and metastases could pave the way to pinpoint novel prognostic markers and targets for therapeutic intervention (Figure 4). It has been proposed that critical signaling pathways that promote pathogenesis may be masked by the over-representation of proliferation-, ER-, and Her2-related signaling signatures in many existing prognostic signatures. Therefore, these minor but critical signaling pathways may be uncovered by studying and distilling normal developmental paradigms such as the fMaSC and fSTR states. Such approaches could potentially deconvolute the complexities of tumor heterogeneity by narrowing the focus on subpopulations of cells that exhibit embryonic signatures and identifying targets specific for these populations.

FIGURE 4
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FIGURE 4. Similarities of genes or signaling pathways critical in embryonic mammary gland development and processes in breast cancer. (A) Key stages in embryonic mammary gland development and genes and their associated pathways involved in the respective stages. For MR-specific developmental processes that are regulated by relevant genes and signaling pathways, please refer to main text. (B,C,D) Processes that are linked to tumorigenesis and metastasis and mediated by the same genes or signaling pathways important for embryonic mammary gland development. Genes in the same signaling pathway are coded in the same colour. Figure created with BioRender.

There are some indications that show embryonic signatures may have clinically-relevant prognostic value. Only a very low percentage of patients who are treated with neoadjuvant chemotherapy will progress to pathological Complete Response (pCR). For this reason, novel approaches that can estimate the probability of pCR are highly desired. The predictive value of normal cellular expression features for pCR was evaluated using univariate and multivariate logistic regression analyses. Indeed, human luminal progenitors (LumProg) and mouse fMaSC expression features were identified as predictive of neoadjuvant chemotherapy efficacy across all breast cancer patients. These signatures were highly expressed in basal-like tumors, consistent with the clinical observation that basal-like tumors have better neoadjuvant chemotherapy response rates. On the other hand, benign luminal A and B tumors which are typically more resistant to neoadjuvant chemotherapy exhibit high expression of another MaSC signature subset (fMaSC-MmEnriched-feature2). Importantly, the prognostic value of these normal and embryonic signatures remained significant even after accounting for tumor intrinsic subtype, proliferative status, and other clinical parameters; in other words, normal cell signatures add information and prognostic value that are distinct from clinical features (Pfefferle et al., 2015). Along a similar vein, a prognostic gene expression signature derived from the E6.5 mouse which is representative of extensive cellular plasticity was shown to predict metastatic competence in human breast tumor cells (Soundararajan et al., 2015).

Conclusion and Perspectives

The misregulation of genes and signaling that are important in regulating normal embryonic mammary gland development frequently occur in pathological conditions such as breast cancer (Howard and Ashworth, 2006). Thus, the identification and study of the mechanisms mediated by genes and signaling pathways during early development may give rise to deeper understanding of disease mechanisms. Notably, embryonic molecular signatures may complement conventional clinical parameters for the stratification of patients and to offer accurate predictions of outcomes among breast cancer patients.

To date, practical considerations and sample limitations have resulted in pooled mammary gland molecular profiling. Considering the unique genetic and developmental history of the mammary glands, such data pooling may have implications in the success of cancer research. Thus, it may be prudent to make MR-specific comparisons and analyses in future research.

Likewise, it will be of interest to further characterize and locate cells harbouring different cell states such as the hybrid state, reminiscent of embryonic cells, in the adult mammary gland and tumors using techniques such as spatial transcriptomics. With recent technical advancements in a suite of multi-omics methods, the identification of the metabolomic and epigenomic states of the embryonic mammary glands—and the parallels to breast cancers - will be exciting themes of research that will illuminate more insights into mammary gland development and cancer pathogenesis.

Author Contributions

MYL wrote the manuscript.

Funding

MYL acknowledges funding from the A*STAR Career Development Fund.

Conflict of Interest

The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Acknowledgments

I would like to thank Jacqueline Veltmaat, Raj Giraddi, Yi Fei Lee and Shirley Lam for helpful discussions and help with illustrations and Rebecca Jackson for editing help.

References

Acevedo, N., Davis, B., Schaeberle, C. M., Sonnenschein, C., and Soto, A. M. (2013). Perinatally Administered Bisphenol a as a Potential Mammary Gland Carcinogen in Rats. Environ. Health Perspect. 121, 1040–1046. doi:10.1289/ehp.1306734

PubMed Abstract | CrossRef Full Text | Google Scholar

Ahmad, N., and Kumar, R. (2011). Steroid Hormone Receptors in Cancer Development: a Target for Cancer Therapeutics. Cancer Lett. 300, 1–9. doi:10.1016/j.canlet.2010.09.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Ahn, Y., Sims, C., Logue, J. M., Weatherbee, S. D., and Krumlauf, R. (2013). Lrp4 and Wise Interplay Controls the Formation and Patterning of Mammary and Other Skin Appendage Placodes by Modulating Wnt Signaling. Development 140, 583–593. doi:10.1242/dev.085118

PubMed Abstract | CrossRef Full Text | Google Scholar

Ai, L., Tao, Q., Zhong, S., Fields, C. R., Kim, W.-J., Lee, M. W., et al. (2006). Inactivation of Wnt Inhibitory Factor-1 (WIF1) Expression by Epigenetic Silencing Is a Common Event in Breast Cancer. Carcinogenesis 27, 1341–1348. doi:10.1093/carcin/bgi379

PubMed Abstract | CrossRef Full Text | Google Scholar

Alarmo, E.-L., and Kallioniemi, A. (2010). Bone Morphogenetic Proteins in Breast Cancer: Dual Role in Tumourigenesis? Endocr. Relat. Cancer 17, R123–R139. doi:10.1677/erc-09-0273

PubMed Abstract | CrossRef Full Text | Google Scholar

Asselin-Labat, M.-L., Sutherland, K. D., Barker, H., Thomas, R., Shackleton, M., Forrest, N. C., et al. (2007). Gata-3 Is an Essential Regulator of Mammary-Gland Morphogenesis and Luminal-Cell Differentiation. Nat. Cell. Biol. 9, 201–209. doi:10.1038/ncb1530

PubMed Abstract | CrossRef Full Text | Google Scholar

Asselin-Labat, M.-L., Sutherland, K. D., Vaillant, F., Gyorki, D. E., Wu, D., Holroyd, S., et al. (2011). Gata-3 Negatively Regulates the Tumor-Initiating Capacity of Mammary Luminal Progenitor Cells and Targets the Putative Tumor Suppressor Caspase-14. Mol. Cell. Biol. 31, 4609–4622. doi:10.1128/mcb.05766-11

PubMed Abstract | CrossRef Full Text | Google Scholar

Aster, J. C., Pear, W. S., and Blacklow, S. C. (2017). The Varied Roles of Notch in Cancer. Annu. Rev. Pathol. Mech. Dis. 12, 245–275. doi:10.1146/annurev-pathol-052016-100127

PubMed Abstract | CrossRef Full Text | Google Scholar

Bach, D.-H., Park, H. J., and Lee, S. K. (2018). The Dual Role of Bone Morphogenetic Proteins in Cancer. Mol. Ther. - Oncolytics 8, 1–13. doi:10.1016/j.omto.2017.10.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Ben-Porath, I., Thomson, M. W., Carey, V. J., Ge, R., Bell, G. W., Regev, A., et al. (2008). An Embryonic Stem Cell-like Gene Expression Signature in Poorly Differentiated Aggressive Human Tumors. Nat. Genet. 40, 499–507. doi:10.1038/ng.127

PubMed Abstract | CrossRef Full Text | Google Scholar

Bhateja, P., Cherian, M., Majumder, S., and Ramaswamy, B. (2019)., 11. Basel). doi:10.3390/cancers11081126The Hedgehog Signaling Pathway: A Viable Target in Breast Cancer?Cancers (Basel)

CrossRef Full Text | Google Scholar

Bolander, F. F. (1990). Differential Characteristics of the Thoracic and Abdominal Mammary Glands from Mice. Exp. Cell. Res. 189, 142–144. doi:10.1016/0014-4827(90)90266-d

PubMed Abstract | CrossRef Full Text | Google Scholar

Boras-Granic, K., Chang, H., Grosschedl, R., and Hamel, P. A. (2006). Lef1 Is Required for the Transition of Wnt Signaling from Mesenchymal to Epithelial Cells in the Mouse Embryonic Mammary Gland. Dev. Biol. 295, 219–231. doi:10.1016/j.ydbio.2006.03.030

PubMed Abstract | CrossRef Full Text | Google Scholar

Boras-Granic, K., Dann, P., and Wysolmerski, J. J. (2014). Embryonic Cells Contribute Directly to the Quiescent Stem Cell Population in the Adult Mouse Mammary Gland. Breast Cancer Res. 16, 487. doi:10.1186/s13058-014-0487-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Bouris, P., Skandalis, S. S., Piperigkou, Z., Afratis, N., Karamanou, K., Aletras, A. J., et al. (2015). Estrogen Receptor Alpha Mediates Epithelial to Mesenchymal Transition, Expression of Specific Matrix Effectors and Functional Properties of Breast Cancer Cells. Matrix Biol. 43, 42–60. doi:10.1016/j.matbio.2015.02.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Briegel, K. (2006). Embryonic Transcription Factors in Human Breast Cancer. IUBMB Life (International Union Biochem. Mol. Biol. Life) 58, 123–132. doi:10.1080/15216540600686870

PubMed Abstract | CrossRef Full Text | Google Scholar

Burbelo, P. D., Miyamoto, S., Utani, A., Brill, S., Yamada, K. M., Hall, A., et al. (1995). p190-B, A New Member of the Rho GAP Family, and Rho are Induced to Cluster After Integrin Cross-Linking. J. Biol. Chem. 270, 30919–30926.

PubMed Abstract | CrossRef Full Text | Google Scholar

Caré, A., Silvani, A., Meccia, E., Mattia, G., Peschle, C., and Colombo, M. P. (1998). Transduction of the SkBr3 Breast Carcinoma Cell Line with the HOXB7 Gene Induces bFGF Expression, Increases Cell Proliferation and Reduces Growth Factor Dependence. Oncogene 16, 3285–3289. doi:10.1038/sj.onc.1201875

PubMed Abstract | CrossRef Full Text | Google Scholar

Carroll, S. B. (1995). Homeotic Genes and the Evolution of Arthropods and Chordates. Nature 376, 479–485. doi:10.1038/376479a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Centonze, A., Lin, S., Tika, E., Sifrim, A., Fioramonti, M., Malfait, M., et al. (2020). Heterotypic Cell-Cell Communication Regulates Glandular Stem Cell Multipotency. Nature 584, 608–613. doi:10.1038/s41586-020-2632-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Chakrabarti, R., Celià-Terrassa, T., Kumar, S., Hang, X., Wei, Y., Choudhury, A., et al. (2018). Notch Ligand Dll1 Mediates Cross-Talk between Mammary Stem Cells and the Macrophageal Niche. Science 360. doi:10.1126/science.aan4153

CrossRef Full Text | Google Scholar

Chang, F., Xing, P., Song, F., du, X., Wang, G., Chen, K., et al. (2016). The Role of T-Box Genes in the Tumorigenesis and Progression of Cancer. Oncol. Lett. 12, 4305–4311. doi:10.3892/ol.2016.5296

PubMed Abstract | CrossRef Full Text | Google Scholar

Chapman, D. L., Garvey, N., Hancock, S., Alexiou, M., Agulnik, S. I., Gibson-Brown, J. J., et al. (1996). Expression of the T-Box Family genes,Tbx1-Tbx5, during Early Mouse Development. Dev. Dyn. 206, 379–390. doi:10.1002/(sici)1097-0177(199608)206:4<379::aid-aja4>3.0.co;2-f

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, D., Zhao, M., and Mundy, G. R. (2004). Bone Morphogenetic Proteins. Growth factors. 22, 233–241. doi:10.1080/08977190412331279890

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, F., and Capecchi, M. R. (1999). Paralogous Mouse Hox Genes, Hoxa9, Hoxb9, and Hoxd9, Function Together to Control Development of the Mammary Gland in Response to Pregnancy. Proc. Natl. Acad. Sci. U.S.A. 96, 541–546. doi:10.1073/pnas.96.2.541

PubMed Abstract | CrossRef Full Text | Google Scholar

Cho, K.-W., Kim, J.-Y., Song, S.-J., Farrell, E., Eblaghie, M. C., Kim, H.-J., et al. (2006). Molecular Interactions between Tbx3 and Bmp4 and a Model for Dorsoventral Positioning of Mammary Gland Development. Proc. Natl. Acad. Sci. U.S.A. 103, 16788–16793. doi:10.1073/pnas.0604645103

PubMed Abstract | CrossRef Full Text | Google Scholar

Chu, E. Y., Hens, J., Andl, T., Kairo, A., Yamaguchi, T. P., Brisken, C., et al. (2004). Canonical WNT Signaling Promotes Mammary Placode Development and Is Essential for Initiation of Mammary Gland Morphogenesis. Development 131, 4819–4829. doi:10.1242/dev.01347

PubMed Abstract | CrossRef Full Text | Google Scholar

Colavito, S. A., Zou, M. R., Yan, Q., Nguyen, D. X., and Stern, D. F. (2014). Significance of Glioma-Associated Oncogene Homolog 1 (GLI1)expression in Claudin-Low Breast Cancer and Crosstalk with the Nuclear Factor Kappa-Light-Chain-Enhancer of Activated B Cells (NFκB) Pathway. Breast Cancer Res. 16, 444. doi:10.1186/s13058-014-0444-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Dai, P., Akimaru, H., Tanaka, Y., Maekawa, T., Nakafuku, M., and Ishii, S. (1999). Sonic Hedgehog-Induced Activation of the Gli1Promoter Is Mediated by GLI3. J. Biol. Chem. 274, 8143–8152. doi:10.1074/jbc.274.12.8143

PubMed Abstract | CrossRef Full Text | Google Scholar

Davenport, T. G., Jerome-Majewska, L. A., and Papaioannou, V. E. (2003). Mammary Gland, Limb and Yolk Sac Defects in Mice lackingTbx3,the Gene Mutated in Human Ulnar Mammary Syndrome. Development 130, 2263–2273. doi:10.1242/dev.00431

PubMed Abstract | CrossRef Full Text | Google Scholar

Davidson, D. (1995). The Function and Evolution of Msx Genes: Pointers and Paradoxes. Trends Genet. 11, 405–411. doi:10.1016/s0168-9525(00)89124-6

PubMed Abstract | CrossRef Full Text | Google Scholar

De Visser, K. E., Ciampricotti, M., Michalak, E. M., Tan, D. W.-M., Speksnijder, E. N., Hau, C.-S., et al. (2012). Developmental Stage-specific Contribution of LGR5+cells to Basal and Luminal Epithelial Lineages in the Postnatal Mammary Gland. J. Pathol. 228, 300–309. doi:10.1002/path.4096

PubMed Abstract | CrossRef Full Text | Google Scholar

Deb, P., Bhan, A., Hussain, I., Ansari, K. I., Bobzean, S. A., Pandita, T. K., et al. (2016). Endocrine Disrupting Chemical, Bisphenol-A, Induces Breast Cancer Associated Gene HOXB9 Expression In Vitro and In Vivo. Gene 590, 234–243. doi:10.1016/j.gene.2016.05.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Du, F., Yuan, P., Wang, T., Zhao, J., Zhao, Z., Luo, Y., et al. (2015). The Significance and Therapeutic Potential of GATA3 Expression and Mutation in Breast Cancer: A Systematic Review. Med. Res. Rev. 35, 1300–1315. doi:10.1002/med.21362

PubMed Abstract | CrossRef Full Text | Google Scholar

Dunbar, M. E., Dann, P. R., Robinson, G. W., Hennighausen, L., Zhang, J. P., and Wysolmerski, J. J. (1999). Parathyroid Hormone-Related Protein Signaling Is Necessary for Sexual Dimorphism during Embryonic Mammary Development. Development 126, 3485–3493. doi:10.1242/dev.126.16.3485

PubMed Abstract | CrossRef Full Text | Google Scholar

Dürnberger, H., and Kratochwil, K. (1980). Specificity of Tissue Interaction and Origin of Mesenchymal Cells in the Androgen Response of the Embryonic Mammary Gland. Cell. 19, 465–471. doi:10.1016/0092-8674(80)90521-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Dwyer, A. R., Truong, T. H., Kerkvliet, C. P., Paul, K. V., Kabos, P., Sartorius, C. A., et al. (2021). Insulin Receptor Substrate-1 (IRS-1) Mediates Progesterone Receptor-Driven Stemness and Endocrine Resistance in Oestrogen Receptor+ Breast Cancer. Br. J. Cancer 124, 217–227. doi:10.1038/s41416-020-01094-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Dydensborg, A. B., Rose, A. A. N., Wilson, B. J., Grote, D., Paquet, M., Giguère, V., et al. (2009). GATA3 Inhibits Breast Cancer Growth and Pulmonary Breast Cancer Metastasis. Oncogene 28, 2634–2642. doi:10.1038/onc.2009.126

PubMed Abstract | CrossRef Full Text | Google Scholar

Eblaghie, M. C., Song, S.-J., Kim, J.-Y., Akita, K., Tickle, C., and Jung, H.-S. (2004). Interactions between FGF and Wnt Signals and Tbx3 Gene Expression in Mammary Gland Initiation in Mouse Embryos. J. Anat. 205, 1–13. doi:10.1111/j.0021-8782.2004.00309.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Eckhardt, B. L., Cao, Y., Redfern, A. D., Chi, L. H., Burrows, A. D., Roslan, S., et al. (2020). Activation of Canonical BMP4-SMAD7 Signaling Suppresses Breast Cancer Metastasis. Cancer Res. 80, 1304–1315. doi:10.1158/0008-5472.can-19-0743

PubMed Abstract | CrossRef Full Text | Google Scholar

Edwards, C. M., and Johnson, R. W. (2021). From Good to Bad: The Opposing Effects of PTHrP on Tumor Growth, Dormancy, and Metastasis throughout Cancer Progression. Front. Oncol. 11, 644303. doi:10.3389/fonc.2021.644303

PubMed Abstract | CrossRef Full Text | Google Scholar

Ekyalongo, R. C., and Yee, D. (2017). Revisiting the IGF-1R as a Breast Cancer Target. NPJ Precis. Oncol. 1. doi:10.1038/s41698-017-0017-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Elias, S., Morgan, M. A., Bikoff, E. K., and Robertson, E. J. (2017). Long-lived Unipotent Blimp1-Positive Luminal Stem Cells Drive Mammary Gland Organogenesis throughout Adult Life. Nat. Commun. 8, 1714. doi:10.1038/s41467-017-01971-w

PubMed Abstract | CrossRef Full Text | Google Scholar

Eyre, R., Alférez, D. G., Santiago-Gómez, A., Spence, K., Mcconnell, J. C., Hart, C., et al. (2019). Microenvironmental IL1β Promotes Breast Cancer Metastatic Colonisation in the Bone via Activation of Wnt Signalling. Nat. Commun. 10, 5016. doi:10.1038/s41467-019-12807-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Feng, Y., Zhang, T., Wang, Y., Xie, M., Ji, X., Luo, X., et al. (2021). Homeobox Genes in Cancers: From Carcinogenesis to Recent Therapeutic Intervention. Front. Oncol. 11, 770428. doi:10.3389/fonc.2021.770428

PubMed Abstract | CrossRef Full Text | Google Scholar

Fiaschi, M., Rozell, B., Bergström, Å., and Toftgård, R. (2009). Development of Mammary Tumors by Conditional Expression of GLI1. Cancer Res. 69, 4810–4817. doi:10.1158/0008-5472.can-08-3938

PubMed Abstract | CrossRef Full Text | Google Scholar

Fillmore, C. M., Gupta, P. B., Rudnick, J. A., Caballero, S., Keller, P. J., Lander, E. S., et al. (2010). Estrogen Expands Breast Cancer Stem-like Cells through Paracrine FGF/Tbx3 Signaling. Proc. Natl. Acad. Sci. U.S.A. 107, 21737–21742. doi:10.1073/pnas.1007863107

PubMed Abstract | CrossRef Full Text | Google Scholar

Foley, J., Dann, P., Hong, J., Cosgrove, J., Dreyer, B., Rimm, D., et al. (2001). Parathyroid Hormone-Related Protein Maintains Mammary Epithelial Fate and Triggers Nipple Skin Differentiation during Embryonic Breast Development. Development 128, 513–525. doi:10.1242/dev.128.4.513

PubMed Abstract | CrossRef Full Text | Google Scholar

Fu, N. Y., Rios, A. C., Pal, B., Law, C. W., Jamieson, P., Liu, R., et al. (2017). Identification of Quiescent and Spatially Restricted Mammary Stem Cells that Are Hormone Responsive. Nat. Cell. Biol. 19, 164–176. doi:10.1038/ncb3471

PubMed Abstract | CrossRef Full Text | Google Scholar

Fuentes, N., and Silveyra, P. (2019). Estrogen Receptor Signaling Mechanisms. Adv. Protein Chem. Struct. Biol. 116, 135–170. doi:10.1016/bs.apcsb.2019.01.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Gallego, M. I., Beachy, P. A., Hennighausen, L., and Robinson, G. W. (2002). Differential Requirements for Shh in Mammary Tissue and Hair Follicle Morphogenesis. Dev. Biol. 249, 131–139. doi:10.1006/dbio.2002.0761

PubMed Abstract | CrossRef Full Text | Google Scholar

Garcia-Closas, M., Gail, M. H., Kelsey, K. T., and Ziegler, R. G. (2013). Searching for Blood DNA Methylation Markers of Breast Cancer Risk and Early Detection. JNCI J. Natl. Cancer Inst. 105, 678–680. doi:10.1093/jnci/djt090

PubMed Abstract | CrossRef Full Text | Google Scholar

Garcia-Gasca, A., and Spyropoulos, D. D. (2000). Differential Mammary Morphogenesis along the Anteroposterior axis in Hoxc6 Gene Targeted Mice. Dev. Dyn. 219, 261–276. doi:10.1002/1097-0177(2000)9999:9999<::AID-DVDY1048>3.0.CO;2-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Gatti, V., Bongiorno-Borbone, L., Fierro, C., Annicchiarico-Petruzzelli, M., Melino, G., and Peschiaroli, A. (2019). p63 at the Crossroads between Stemness and Metastasis in Breast Cancer. Int. J. Mol. Sci. 20. doi:10.3390/ijms20112683

PubMed Abstract | CrossRef Full Text | Google Scholar

Gibson, S. L., Ma, Z., and Shaw, L. M. (2007). Divergent Roles for IRS-1 and IRS-2 in Breast Cancer Metastasis. Cell. Cycle 6, 631–637. doi:10.4161/cc.6.6.3987

PubMed Abstract | CrossRef Full Text | Google Scholar

Gilbert, P. M., Mouw, J. K., Unger, M. A., Lakins, J. N., Gbegnon, M. K., Clemmer, V. B., et al. (2010). HOXA9 Regulates BRCA1 Expression to Modulate Human Breast Tumor Phenotype. J. Clin. Investig. 120, 1535–1550. doi:10.1172/jci39534

PubMed Abstract | CrossRef Full Text | Google Scholar

Giraddi, R. R., Chung, C.-Y., Heinz, R. E., Balcioglu, O., Novotny, M., Trejo, C. L., et al. (2018). Single-Cell Transcriptomes Distinguish Stem Cell State Changes and Lineage Specification Programs in Early Mammary Gland Development. Cell. Rep. 24, 1653–1666. doi:10.1016/j.celrep.2018.07.025

PubMed Abstract | CrossRef Full Text | Google Scholar

Gu, B., Sun, P., Yuan, Y., Moraes, R. C., Li, A., Teng, A., et al. (2009). Pygo2 Expands Mammary Progenitor Cells by Facilitating Histone H3 K4 Methylation. J. Cell. Biol. 185, 811–826. doi:10.1083/jcb.200810133

PubMed Abstract | CrossRef Full Text | Google Scholar

Guise, T. A., Yin, J. J., Taylor, S. D., Kumagai, Y., Dallas, M., Boyce, B. F., et al. (1996). Evidence for a Causal Role of Parathyroid Hormone-Related Protein in the Pathogenesis of Human Breast Cancer-Mediated Osteolysis. J. Clin. Investig. 98, 1544–1549. doi:10.1172/jci118947

PubMed Abstract | CrossRef Full Text | Google Scholar

Guise, T. A., Yin, J. J., Thomas, R. J., Dallas, M., Cui, Y., and Gillespie, M. T. (2002). Parathyroid Hormone-Related Protein (PTHrP)-(1-139) Isoform Is Efficiently Secreted In Vitro and Enhances Breast Cancer Metastasis to Bone In Vivo. Bone 30, 670–676. doi:10.1016/s8756-3282(02)00685-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Hatsell, S. J., and Cowin, P. (2006). Gli3-mediated Repression of Hedgehog Targets Is Required for Normal Mammary Development. Development 133, 3661–3670. doi:10.1242/dev.02542

PubMed Abstract | CrossRef Full Text | Google Scholar

Hayes, N. V. L., and Gullick, W. J. (2008). The Neuregulin Family of Genes and Their Multiple Splice Variants in Breast Cancer. J. Mammary Gland. Biol. Neoplasia 13, 205–214. doi:10.1007/s10911-008-9078-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Heckman, B. M., Chakravarty, G., Vargo-Gogola, T., Gonzales-Rimbau, M., Hadsell, D. L., Lee, A. V., et al. (2007). Crosstalk between the P190-B RhoGAP and IGF Signaling Pathways Is Required for Embryonic Mammary Bud Development. Dev. Biol. 309, 137–149. doi:10.1016/j.ydbio.2007.07.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Heckman-Stoddard, B. M., Vargo-Gogola, T., Mchenry, P. R., Jiang, V., Herrick, M. P., Hilsenbeck, S. G., et al. (2009). Haploinsufficiency for p190B RhoGAP Inhibits MMTV-Neu Tumor Progression. Breast Cancer Res. 11, R61. doi:10.1186/bcr2352

PubMed Abstract | CrossRef Full Text | Google Scholar

Henderson, M. A., Danks, J. A., Slavin, J. L., Byrnes, G. B., Choong, P. F. M., Spillane, J. B., et al. (2006). Parathyroid Hormone-Related Protein Localization in Breast Cancers Predict Improved Prognosis. Cancer Res. 66, 2250–2256. doi:10.1158/0008-5472.can-05-2814

PubMed Abstract | CrossRef Full Text | Google Scholar

Hens, J. R., Dann, P., Zhang, J.-P., Harris, S., Robinson, G. W., and Wysolmerski, J. (2007). BMP4 and PTHrP Interact to Stimulate Ductal Outgrowth during Embryonic Mammary Development and to Inhibit Hair Follicle Induction. Development 134, 1221–1230. doi:10.1242/dev.000182

PubMed Abstract | CrossRef Full Text | Google Scholar

Hijazi, M. M., Young, P. E., Dougherty, M. K., Bressette, D. S., Cao, T. T., Pierce, J. H., et al. (1998). NRG-3 in Human Breast Cancers: Activation of Multiple erbB Family Proteins. Int. J. Oncol. 13, 1061–1067. doi:10.3892/ijo.13.5.1061

PubMed Abstract | CrossRef Full Text | Google Scholar

Hiremath, M., Dann, P., Fischer, J., Butterworth, D., Boras-Granic, K., Hens, J., et al. (2012). Parathyroid Hormone-Related Protein Activates Wnt Signaling to Specify the Embryonic Mammary Mesenchyme. Development 139, 4239–4249. doi:10.1242/dev.080671

PubMed Abstract | CrossRef Full Text | Google Scholar

Hiremath, M., and Wysolmerski, J. (2013). Parathyroid Hormone-Related Protein Specifies the Mammary Mesenchyme and Regulates Embryonic Mammary Development. J. Mammary Gland. Biol. Neoplasia 18, 171–177. doi:10.1007/s10911-013-9283-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Hori, K., Sen, A., and Artavanis-Tsakonas, S. (2013). Notch Signaling at a Glance. J. Cell. Sci. 126, 2135–2140. doi:10.1242/jcs.127308

PubMed Abstract | CrossRef Full Text | Google Scholar

Howard, B., and Ashworth, A. (2006). Signalling Pathways Implicated in Early Mammary Gland Morphogenesis and Breast Cancer. PLoS Genet. 2, e112. doi:10.1371/journal.pgen.0020112

PubMed Abstract | CrossRef Full Text | Google Scholar

Howard, B., Panchal, H., Mccarthy, A., and Ashworth, A. (2005). Identification of the Scaramanga Gene Implicates Neuregulin3 in Mammary Gland Specification. Genes. Dev. 19, 2078–2090. doi:10.1101/gad.338505

PubMed Abstract | CrossRef Full Text | Google Scholar

Hu, C., Diévart, A., Lupien, M., Calvo, E., Tremblay, G., and Jolicoeur, P. (2006). Overexpression of Activated Murine Notch1 and Notch3 in Transgenic Mice Blocks Mammary Gland Development and Induces Mammary Tumors. Am. J. Pathology 168, 973–990. doi:10.2353/ajpath.2006.050416

CrossRef Full Text | Google Scholar

Huang, P., Chen, A., He, W., Li, Z., Zhang, G., Liu, Z., et al. (2017). BMP-2 Induces EMT and Breast Cancer Stemness through Rb and CD44. Cell. Death Discov. 3, 17039. doi:10.1038/cddiscovery.2017.39

PubMed Abstract | CrossRef Full Text | Google Scholar

Humke, E. W., Dorn, K. V., Milenkovic, L., Scott, M. P., and Rohatgi, R. (2010). The Output of Hedgehog Signaling Is Controlled by the Dynamic Association between Suppressor of Fused and the Gli Proteins. Genes. Dev. 24, 670–682. doi:10.1101/gad.1902910

PubMed Abstract | CrossRef Full Text | Google Scholar

Hussain, I., Bhan, A., Ansari, K. I., Deb, P., Bobzean, S. A. M., Perrotti, L. I., et al. (2015). Bisphenol-A Induces Expression of HOXC6, an Estrogen-Regulated Homeobox-Containing Gene Associated with Breast Cancer. Biochimica Biophysica Acta (BBA) - Gene Regul. Mech. 1849, 697–708. doi:10.1016/j.bbagrm.2015.02.003

CrossRef Full Text | Google Scholar

Incassati, A., Chandramouli, A., Eelkema, R., and Cowin, P. (2010). Key Signaling Nodes in Mammary Gland Development and Cancer: β-catenin. Breast Cancer Res. 12, 213. doi:10.1186/bcr2723

PubMed Abstract | CrossRef Full Text | Google Scholar

Itasaki, N., Jones, C. M., Mercurio, S., Rowe, A., Domingos, P. M., Smith, J. C., et al. (2003). Wise, a Context-dependent Activator and Inhibitor of Wnt Signalling. Development 130, 4295–4305. doi:10.1242/dev.00674

PubMed Abstract | CrossRef Full Text | Google Scholar

Javelaud, D., Alexaki, V. I., Dennler, S., Mohammad, K. S., Guise, T. A., and Mauviel, A. (2011). TGF-β/SMAD/GLI2 Signaling Axis in Cancer Progression and Metastasis: Figure 1. Cancer Res. 71, 5606–5610. doi:10.1158/0008-5472.can-11-1194

PubMed Abstract | CrossRef Full Text | Google Scholar

Jerome-Majewska, L. A., Jenkins, G. P., Ernstoff, E., Zindy, F., Sherr, C. J., and Papaioannou, V. E. (2005). Tbx3, the Ulnar-Mammary Syndrome Gene, andTbx2interact in Mammary Gland Development through a p19Arf/p53-independent Pathway. Dev. Dyn. 234, 922–933. doi:10.1002/dvdy.20575

PubMed Abstract | CrossRef Full Text | Google Scholar

Kelleher, F. C., Fennelly, D., and Rafferty, M. (2006). Common Critical Pathways in Embryogenesis and Cancer. Acta Oncol. 45, 375–388. doi:10.1080/02841860600602946

PubMed Abstract | CrossRef Full Text | Google Scholar

Kimelman, D., and Xu, W. (2006). β-Catenin Destruction Complex: Insights and Questions from a Structural Perspective. Oncogene 25, 7482–7491. doi:10.1038/sj.onc.1210055

PubMed Abstract | CrossRef Full Text | Google Scholar

Kise, Y., Morinaka, A., Teglund, S., and Miki, H. (2009). Sufu Recruits GSK3β for Efficient Processing of Gli3. Biochem. Biophysical Res. Commun. 387, 569–574. doi:10.1016/j.bbrc.2009.07.087

PubMed Abstract | CrossRef Full Text | Google Scholar

Kogata, N., Oliemuller, E., Wansbury, O., and Howard, B. A. (2014). Neuregulin-3Regulates Epithelial Progenitor Cell Positioning and Specifies Mammary Phenotype. Stem Cells Dev. 23, 2758–2770. doi:10.1089/scd.2014.0082

PubMed Abstract | CrossRef Full Text | Google Scholar

Kopan, R., and Ilagan, M. X. G. (2009). The Canonical Notch Signaling Pathway: Unfolding the Activation Mechanism. Cell. 137, 216–233. doi:10.1016/j.cell.2009.03.045

PubMed Abstract | CrossRef Full Text | Google Scholar

Koren, S., Reavie, L., Couto, J. P., de Silva, D., Stadler, M. B., Roloff, T., et al. (2015). PIK3CAH1047R Induces Multipotency and Multi-Lineage Mammary Tumours. Nature 525, 114–118. doi:10.1038/nature14669

PubMed Abstract | CrossRef Full Text | Google Scholar

Kretzschmar, K., and Watt, F. M. (2012). Lineage Tracing. Cell. 148, 33–45. doi:10.1016/j.cell.2012.01.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Krishna, B. M., Jana, S., Singhal, J., Horne, D., Awasthi, S., Salgia, R., et al. (2019). Notch Signaling in Breast Cancer: From Pathway Analysis to Therapy. Cancer Lett. 461, 123–131. doi:10.1016/j.canlet.2019.07.012

PubMed Abstract | CrossRef Full Text | Google Scholar

Kubo, M., Nakamura, M., Tasaki, A., Yamanaka, N., Nakashima, H., Nomura, M., et al. (2004). Hedgehog Signaling Pathway Is a New Therapeutic Target for Patients with Breast Cancer. Cancer Res. 64, 6071–6074. doi:10.1158/0008-5472.can-04-0416

PubMed Abstract | CrossRef Full Text | Google Scholar

Lafkas, D., Rodilla, V., Huyghe, M., Mourao, L., Kiaris, H., and Fre, S. (2013). Notch3 Marks Clonogenic Mammary Luminal Progenitor Cells In Vivo. J. Cell. Biol. 203, 47–56. doi:10.1083/jcb.201307046

PubMed Abstract | CrossRef Full Text | Google Scholar

Lamy, M., Ferreira, A., Dias, J. S., Braga, S., Silva, G., and Barbas, A. (2017). Notch-out for Breast Cancer Therapies. New Biotechnol. 39, 215–221. doi:10.1016/j.nbt.2017.08.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Laurikkala, J., Kassai, Y., Pakkasjärvi, L., Thesleff, I., and Itoh, N. (2003). Identification of a Secreted BMP Antagonist, Ectodin, Integrating BMP, FGF, and SHH Signals from the Tooth Enamel Knot. Dev. Biol. 264, 91–105. doi:10.1016/j.ydbio.2003.08.011

PubMed Abstract | CrossRef Full Text | Google Scholar

Lawson, D. A., Bhakta, N. R., Kessenbrock, K., Prummel, K. D., Yu, Y., Takai, K., et al. (2015). Single-cell Analysis Reveals a Stem-Cell Program in Human Metastatic Breast Cancer Cells. Nature 526, 131–135. doi:10.1038/nature15260

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, M. Y., Giraddi, R. R., and Tam, W. L. (2019). Cancer Stem Cells: Concepts, Challenges, and Opportunities for Cancer Therapy. Methods Mol. Biol., 43–66. doi:10.1007/978-1-4939-9524-0_4

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, M. Y., Racine, V., Jagadpramana, P., Sun, L., Yu, W., du, T., et al. (2011). Ectodermal Influx and Cell Hypertrophy Provide Early Growth for All Murine Mammary Rudiments, and Are Differentially Regulated Among Them by Gli3. PLoS One 6, e26242. doi:10.1371/journal.pone.0026242

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, M. Y., Sun, L., and Veltmaat, J. M. (2013). Hedgehog and Gli Signaling in Embryonic Mammary Gland Development. J. Mammary Gland. Biol. Neoplasia 18, 133–138. doi:10.1007/s10911-013-9291-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Leitner, B. P., Siebel, S., Akingbesote, N. D., Zhang, X., and Perry, R. J. (2022). Insulin and Cancer: a Tangled Web. Biochem. J. 479, 583–607. doi:10.1042/bcj20210134

PubMed Abstract | CrossRef Full Text | Google Scholar

Lewis, M. T. (2000). Homeobox Genes in Mammary Gland Development and Neoplasia. Breast Cancer Res. 2, 158–169. doi:10.1186/bcr49

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, B., Huang, Q., and Wei, G. H. (2019). The Role of HOX Transcription Factors in Cancer Predisposition and Progression, 11. Cancers (Basel). doi:10.3390/cancers11040528

CrossRef Full Text | Google Scholar

Li, J., Karaplis, A. C., Huang, D. C., Siegel, P. M., Camirand, A., Yang, X. F., et al. (2011). PTHrP Drives Breast Tumor Initiation, Progression, and Metastasis in Mice and Is a Potential Therapy Target. J. Clin. Investig. 121, 4655–4669. doi:10.1172/jci46134

PubMed Abstract | CrossRef Full Text | Google Scholar

Lilja, A. M., Rodilla, V., Huyghe, M., Hannezo, E., Landragin, C., Renaud, O., et al. (2018). Clonal Analysis of Notch1-Expressing Cells Reveals the Existence of Unipotent Stem Cells that Retain Long-Term Plasticity in the Embryonic Mammary Gland. Nat. Cell. Biol. 20, 677–687. doi:10.1038/s41556-018-0108-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Lin, M., Zhu, H., Shen, Q., Sun, L.-Z., and Zhu, X. (2022). GLI3 and Androgen Receptor Are Mutually Dependent for Their Malignancy-Promoting Activity in Ovarian and Breast Cancer Cells. Cell. Signal. 92, 110278. doi:10.1016/j.cellsig.2022.110278

PubMed Abstract | CrossRef Full Text | Google Scholar

Lin, S.-Y., Xia, W., Wang, J. C., Kwong, K. Y., Spohn, B., Wen, Y., et al. (2000). β-Catenin, a Novel Prognostic Marker for Breast Cancer: Its Roles in Cyclin D1 Expression and Cancer Progression. Proc. Natl. Acad. Sci. U.S.A. 97, 4262–4266. doi:10.1073/pnas.060025397

PubMed Abstract | CrossRef Full Text | Google Scholar

Lindvall, C., Evans, N. C., Zylstra, C. R., Li, Y., Alexander, C. M., and Williams, B. O. (2006). The Wnt Signaling Receptor Lrp5 Is Required for Mammary Ductal Stem Cell Activity and Wnt1-Induced Tumorigenesis. J. Biol. Chem. 281, 35081–35087. doi:10.1074/jbc.m607571200

PubMed Abstract | CrossRef Full Text | Google Scholar

Lindvall, C., Zylstra, C. R., Evans, N., West, R. A., Dykema, K., Furge, K. A., et al. (2009). The Wnt Co-receptor Lrp6 Is Required for Normal Mouse Mammary Gland Development. PLoS One 4, e5813. doi:10.1371/journal.pone.0005813

PubMed Abstract | CrossRef Full Text | Google Scholar

Lintern, K. B., Guidato, S., Rowe, A., Saldanha, J. W., and Itasaki, N. (2009). Characterization of Wise Protein and its Molecular Mechanism to Interact with Both Wnt and BMP Signals. J. Biol. Chem. 284, 23159–23168. doi:10.1074/jbc.m109.025478

PubMed Abstract | CrossRef Full Text | Google Scholar

Lodillinsky, C., Infante, E., Guichard, A., Chaligné, R., Fuhrmann, L., Cyrta, J., et al. (2016). p63/MT1-MMP axis Is Required for In Situ to Invasive Transition in Basal-like Breast Cancer. Oncogene 35, 344–357. doi:10.1038/onc.2015.87

PubMed Abstract | CrossRef Full Text | Google Scholar

Lowery, J. W., and Rosen, V. (2018). Bone Morphogenetic Protein-Based Therapeutic Approaches. Cold Spring Harb. Perspect. Biol. 10. doi:10.1101/cshperspect.a022327

PubMed Abstract | CrossRef Full Text | Google Scholar

Ma, Z., Gibson, S. L., Byrne, M. A., Zhang, J., White, M. F., and Shaw, L. M. (2006). Suppression of Insulin Receptor Substrate 1 (IRS-1) Promotes Mammary Tumor Metastasis. Mol. Cell. Biol. 26, 9338–9351. doi:10.1128/mcb.01032-06

PubMed Abstract | CrossRef Full Text | Google Scholar

Mailleux, A. A., Spencer-Dene, B., Dillon, C., Ndiaye, D., Savona-Baron, C., Itoh, N., et al. (2002). Role of FGF10/FGFR2b Signaling during Mammary Gland Development in the Mouse Embryo. Development 129, 53–60. doi:10.1242/dev.129.1.53

PubMed Abstract | CrossRef Full Text | Google Scholar

Makarem, M., Kannan, N., Nguyen, L. V., Knapp, D. J. H. F., Balani, S., Prater, M. D., et al. (2013). Developmental Changes in the In Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells. PLoS Biol. 11, e1001630. doi:10.1371/journal.pbio.1001630

PubMed Abstract | CrossRef Full Text | Google Scholar

Manavathi, B., Dey, O., Gajulapalli, V. N. R., Bhatia, R. S., Bugide, S., and Kumar, R. (2013). Derailed Estrogen Signaling and Breast Cancer: an Authentic Couple. Endocr. Rev. 34, 1–32. doi:10.1210/er.2011-1057

PubMed Abstract | CrossRef Full Text | Google Scholar

Massah, S., Foo, J., Li, N., Truong, S., Nouri, M., Xie, L., et al. (2021). Gli Activation by the Estrogen Receptor in Breast Cancer Cells: Regulation of Cancer Cell Growth by Gli3. Mol. Cell. Endocrinol. 522, 111136. doi:10.1016/j.mce.2020.111136

PubMed Abstract | CrossRef Full Text | Google Scholar

Matsuo, J., Mon, N. N., Douchi, D., Yamamura, A., Kulkarni, M., Heng, D. L., et al. (2022). A Runx1-Enhancer Element eR1 Identified Lineage Restricted Mammary Luminal Stem Cells. Stem Cells 40, 112–122. doi:10.1093/stmcls/sxab009

PubMed Abstract | CrossRef Full Text | Google Scholar

Mchenry, P. R., Sears, J. C., Herrick, M. P., Chang, P., Heckman-Stoddard, B. M., Rybarczyk, M., et al. (2010). P190B RhoGAP Has Pro-tumorigenic Functions during MMTV-Neu Mammary Tumorigenesis and Metastasis. Breast Cancer Res. 12, R73. doi:10.1186/bcr2643

PubMed Abstract | CrossRef Full Text | Google Scholar

Mcleskey, S. W., Tobias, C. A., Vezza, P. R., Filie, A. C., Kern, F. G., and Hanfelt, J. (1998). Tumor Growth of FGF or VEGF Transfected MCF-7 Breast Carcinoma Cells Correlates with Density of Specific Microvessels Independent of the Transfected Angiogenic Factor. Am. J. Pathology 153, 1993–2006. doi:10.1016/s0002-9440(10)65713-6

CrossRef Full Text | Google Scholar

Melino, G. (2011). p63 Is a Suppressor of Tumorigenesis and Metastasis Interacting with Mutant P53. Cell. Death Differ. 18, 1487–1499. doi:10.1038/cdd.2011.81

PubMed Abstract | CrossRef Full Text | Google Scholar

Memmi, E. M., Sanarico, A. G., Giacobbe, A., Peschiaroli, A., Frezza, V., Cicalese, A., et al. (2015). p63 Sustains Self-Renewal of Mammary Cancer Stem Cells through Regulation of Sonic Hedgehog Signaling. Proc. Natl. Acad. Sci. U.S.A. 112, 3499–3504. doi:10.1073/pnas.1500762112

PubMed Abstract | CrossRef Full Text | Google Scholar

Meurette, O., and Mehlen, P. (2018). Notch Signaling in the Tumor Microenvironment. Cancer Cell. 34, 536–548. doi:10.1016/j.ccell.2018.07.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Michailidou, K., Hall, P., Gonzalez-Neira, A., Ghoussaini, M., Dennis, J., Milne, R. L., et al. 2013. Large-scale Genotyping Identifies 41 New Loci Associated with Breast Cancer Risk. Nat. Genet., 45, 353-61, 361e1-2.doi:10.1038/ng.2563

PubMed Abstract | CrossRef Full Text | Google Scholar

Michno, K., Boras-Granic, K., Mill, P., Hui, C. C., and Hamel, P. A. (2003). Shh Expression Is Required for Embryonic Hair Follicle but Not Mammary Gland Development. Dev. Biol. 264, 153–165. doi:10.1016/s0012-1606(03)00401-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Miettinen, M., Mccue, P. A., Sarlomo-Rikala, M., Rys, J., Czapiewski, P., Wazny, K., et al. (2014). GATA3: a Multispecific but Potentially Useful Marker in Surgical Pathology: a Systematic Analysis of 2500 Epithelial and Nonepithelial Tumors. Am. J. Surg. Pathol. 38, 13–22. doi:10.1097/pas.0b013e3182a0218f

PubMed Abstract | CrossRef Full Text | Google Scholar

Mikkola, M. L., and Thesleff, I. (2003). Ectodysplasin Signaling in Development. Cytokine Growth Factor Rev. 14, 211–224. doi:10.1016/s1359-6101(03)00020-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Mills, A. A., Zheng, B., Wang, X. J., Vogel, H., Roop, D. R., and Bradley, A. (1999). p63 Is a P53 Homologue Required for Limb and Epidermal Morphogenesis. Nature 398, 708–713. doi:10.1038/19531

PubMed Abstract | CrossRef Full Text | Google Scholar

Minasian, H. (1983). Regional Differences in Blood Flow in Relation to Tumour Growth in the Mouse. Lab. Anim. 17, 21–24. doi:10.1258/002367783781070993

PubMed Abstract | CrossRef Full Text | Google Scholar

Mohammadi Ghahhari, N., Sznurkowska, M. K., Hulo, N., Bernasconi, L., Aceto, N., and Picard, D. (2022). Cooperative Interaction between ERalpha and the EMT-Inducer ZEB1 Reprograms Breast Cancer Cells for Bone Metastasis. Nat. Commun. 13, 2104. doi:10.1038/s41467-022-29723-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Mohammadi-Yeganeh, S., Mansouri, A., and Paryan, M. (2015). Targeting of miR9/NOTCH1 Interaction Reduces Metastatic Behavior in Triple-Negative Breast Cancer. Chem. Biol. Drug Des. 86, 1185–1191. doi:10.1111/cbdd.12584

PubMed Abstract | CrossRef Full Text | Google Scholar

Morgan, B. A. (1997). Hox Genes and Embryonic Development. Poult. Sci. 76, 96–104. doi:10.1093/ps/76.1.96

PubMed Abstract | CrossRef Full Text | Google Scholar

Morgan, R., Boxall, A., Harrington, K. J., Simpson, G. R., Gillett, C., Michael, A., et al. (2012). Targeting the HOX/PBX Dimer in Breast Cancer. Breast Cancer Res. Treat. 136, 389–398. doi:10.1007/s10549-012-2259-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Mosca, E., Bertoli, G., Piscitelli, E., Vilardo, L., Reinbold, R. A., Zucchi, I., et al. (2009). Identification of Functionally Related Genes Using Data Mining and Data Integration: a Breast Cancer Case Study. BMC Bioinforma. 10 (Suppl. 12), S8. doi:10.1186/1471-2105-10-S12-S8

CrossRef Full Text | Google Scholar

Mustonen, T., Ilmonen, M., Pummila, M., Kangas, A. T., Laurikkala, J., Jaatinen, R., et al. (2004). Ectodysplasin A1 Promotes Placodal Cell Fate during Early Morphogenesis of Ectodermal Appendages. Development 131, 4907–4919. doi:10.1242/dev.01377

PubMed Abstract | CrossRef Full Text | Google Scholar

Mustonen, T., Pispa, J., Mikkola, M. L., Pummila, M., Kangas, A. T., Pakkasjarvi, L., et al. (2003). Stimulation of Ectodermal Organ Development by Ectodysplasin-A1. Dev. Biol. 259, 123–136. doi:10.1016/s0012-1606(03)00157-x

PubMed Abstract | CrossRef Full Text | Google Scholar

Nagle, J. A., Ma, Z., Byrne, M. A., White, M. F., and Shaw, L. M. (2004). Involvement of Insulin Receptor Substrate 2 in Mammary Tumor Metastasis. Mol. Cell. Biol. 24, 9726–9735. doi:10.1128/mcb.24.22.9726-9735.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

Nandi, A., and Chakrabarti, R. (2020). The Many Facets of Notch Signaling in Breast Cancer: toward Overcoming Therapeutic Resistance. Genes. Dev. 34, 1422–1438. doi:10.1101/gad.342287.120

PubMed Abstract | CrossRef Full Text | Google Scholar

Nusse, R., and Clevers, H. (2017). Wnt/beta-Catenin Signaling, Disease, and Emerging Therapeutic Modalities. Cell. 169, 985–999. doi:10.1016/j.cell.2017.05.016

PubMed Abstract | CrossRef Full Text | Google Scholar

Nusse, R., and Varmus, H. E. (1982). Many Tumors Induced by the Mouse Mammary Tumor Virus Contain a Provirus Integrated in the Same Region of the Host Genome. Cell. 31, 99–109. doi:10.1016/0092-8674(82)90409-3

PubMed Abstract | CrossRef Full Text | Google Scholar

O'toole, S. A., Machalek, D. A., Shearer, R. F., Millar, E. K., Nair, R., Schofield, P., et al. (2011). Hedgehog Overexpression Is Associated with Stromal Interactions and Predicts for Poor Outcome in Breast Cancer. Cancer Res. 71, 4002–4014. doi:10.1158/0008-5472.can-10-3738

PubMed Abstract | CrossRef Full Text | Google Scholar

Ocana, A., Diez-Gonzalez, L., Esparis-Ogando, A., Montero, J. C., Amir, E., and Pandiella, A. (2016). Neuregulin Expression in Solid Tumors: Prognostic Value and Predictive Role to Anti-HER3 Therapies. Oncotarget 7, 45042–45051. doi:10.18632/oncotarget.8648

PubMed Abstract | CrossRef Full Text | Google Scholar

Panchal, H., Wansbury, O., Parry, S., Ashworth, A., and Howard, B. (2007). Neuregulin3 Alters Cell Fate in the Epidermis and Mammary Gland. BMC Dev. Biol. 7, 105. doi:10.1186/1471-213x-7-105

PubMed Abstract | CrossRef Full Text | Google Scholar

Peng, J., and Zhang, D. (2021). Coexpression of EphA10 and Gli3 Promotes Breast Cancer Cell Proliferation, Invasion and Migration. J. Investig. Med. 69, 1215–1221. doi:10.1136/jim-2021-001836

PubMed Abstract | CrossRef Full Text | Google Scholar

Pfefferle, A. D., Spike, B. T., Wahl, G. M., and Perou, C. M. (2015). Luminal Progenitor and Fetal Mammary Stem Cell Expression Features Predict Breast Tumor Response to Neoadjuvant Chemotherapy. Breast Cancer Res. Treat. 149, 425–437. doi:10.1007/s10549-014-3262-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Phippard, D. J., Weber-Hall, S. J., Sharpe, P. T., Naylor, M. S., Jayatalake, H., Maas, R., et al. (1996). Regulation of Msx-1, Msx-2, Bmp-2 and Bmp-4 during Foetal and Postnatal Mammary Gland Development. Development 122, 2729–2737. doi:10.1242/dev.122.9.2729

PubMed Abstract | CrossRef Full Text | Google Scholar

Pispa, J., Mikkola, M. L., Mustonen, T., and Thesleff, I. (2003). Ectodysplasin, Edar and TNFRSF19 Are Expressed in Complementary and Overlapping Patterns during Mouse Embryogenesis. Gene Expr. Patterns 3, 675–679. doi:10.1016/s1567-133x(03)00092-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Pownall, M. E., and Isaacs, H. V. (2010). FGF Signalling in Vertebrate Development. San Rafael (CA).

Google Scholar

Prater, M. D., Petit, V., Alasdair Russell, I., Giraddi, R. R., Shehata, M., Menon, S., et al. (2014). Mammary Stem Cells Have Myoepithelial Cell Properties. Nat. Cell. Biol. 16 (942-50), 1–7. doi:10.1038/ncb3025

CrossRef Full Text | Google Scholar

Prince, S., Carreira, S., Vance, K. W., Abrahams, A., and Goding, C. R. (2004). Tbx2 Directly Represses the Expression of the p21(WAF1) Cyclin-dependent Kinase Inhibitor. Cancer Res. 64, 1669–1674. doi:10.1158/0008-5472.can-03-3286

PubMed Abstract | CrossRef Full Text | Google Scholar

Rieger, M. E., Sims, A. H., Coats, E. R., Clarke, R. B., and Briegel, K. J. (2010). The Embryonic Transcription Cofactor LBH Is a Direct Target of the Wnt Signaling Pathway in Epithelial Development and in Aggressive Basal Subtype Breast Cancers. Mol. Cell. Biol. 30, 4267–4279. doi:10.1128/mcb.01418-09

PubMed Abstract | CrossRef Full Text | Google Scholar

Riobo-Del Galdo, N. A., Lara Montero, A., and Wertheimer, E. V. (2019). Role of Hedgehog Signaling in Breast Cancer: Pathogenesis and Therapeutics. Cells 8. doi:10.3390/cells8040375

PubMed Abstract | CrossRef Full Text | Google Scholar

Rios, A. C., Fu, N. Y., Lindeman, G. J., and Visvader, J. E. (2014). In Situ identification of Bipotent Stem Cells in the Mammary Gland. Nature 506, 322–327. doi:10.1038/nature12948

PubMed Abstract | CrossRef Full Text | Google Scholar

Rodilla, V., Dasti, A., Huyghe, M., Lafkas, D., Laurent, C., Reyal, F., et al. (2015). Luminal Progenitors Restrict Their Lineage Potential during Mammary Gland Development. PLoS Biol. 13, e1002069. doi:10.1371/journal.pbio.1002069

PubMed Abstract | CrossRef Full Text | Google Scholar

Rowley, M., Grothey, E., and Couch, F. J. (2004). The Role of Tbx2 and Tbx3 in Mammary Development and Tumorigenesis. J. Mammary Gland. Biol. Neoplasia 9, 109–118. doi:10.1023/b:jomg.0000037156.64331.3f

PubMed Abstract | CrossRef Full Text | Google Scholar

Russo, I. H., and Russo, J. (1988). Hormone Prevention of Mammary Carcinogenesis: a New Approach in Anticancer Research. Anticancer Res. 8, 1247–1264.

PubMed Abstract | Google Scholar

Saha Roy, S., and Vadlamudi, R. K. (20122012). Role of Estrogen Receptor Signaling in Breast Cancer Metastasis. Int. J. Breast Cancer, 654698. doi:10.1155/2012/654698

PubMed Abstract | CrossRef Full Text | Google Scholar

Sakakura, T., Sakagami, Y., and Nishizuka, Y. (1982). Dual Origin of Mesenchymal Tissues Participating in Mouse Mammary Gland Embryogenesis. Dev. Biol. 91, 202–207. doi:10.1016/0012-1606(82)90024-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Santolla, M. F., and Maggiolini, M. (2020). The FGF/FGFR System in Breast Cancer: Oncogenic Features and Therapeutic Perspectives, 12. Cancers (Basel). doi:10.3390/cancers12103029

CrossRef Full Text | Google Scholar

Satokata, I., Ma, L., Ohshima, H., Bei, M., Woo, I., Nishizawa, K., et al. (2000). Msx2 Deficiency in Mice Causes Pleiotropic Defects in Bone Growth and Ectodermal Organ Formation. Nat. Genet. 24, 391–395. doi:10.1038/74231

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmidt, H. (1998). Supernumerary Nipples: Prevalence, Size, Sex and Side Predilection -- a Prospective Clinical Study. Eur. J. Pediatr. 157, 821–823. doi:10.1007/s004310050944

PubMed Abstract | CrossRef Full Text | Google Scholar

Shan, L., Li, X., Liu, L., Ding, X., Wang, Q., Zheng, Y., et al. (2014). GATA3 Cooperates with PARP1 to Regulate CCND1 Transcription through Modulating Histone H1 Incorporation. Oncogene 33, 3205–3216. doi:10.1038/onc.2013.270

PubMed Abstract | CrossRef Full Text | Google Scholar

Sheldon, W. G., Owen, K., Weed, L., and Kodell, R. (1982). Distribution of Mammary Gland Neoplasms and Factors Influencing Metastases in Hybrid Mice. Lab. Anim. Sci. 32, 166–168.

PubMed Abstract | Google Scholar

Siebel, C., and Lendahl, U. (2017). Notch Signaling in Development, Tissue Homeostasis, and Disease. Physiol. Rev. 97, 1235–1294. doi:10.1152/physrev.00005.2017

PubMed Abstract | CrossRef Full Text | Google Scholar

Siersbaek, R., Kumar, S., and Carroll, J. S. (2018). Signaling Pathways and Steroid Receptors Modulating Estrogen Receptor Alpha Function in Breast Cancer. Genes. Dev. 32, 1141–1154.

PubMed Abstract | Google Scholar

Sinclair, C. S., Rowley, M., Naderi, A., and Couch, F. J. (2003). The 17q23 Amplicon and Breast Cancer. Breast Cancer Res. Treat. 78, 313–322. doi:10.1023/a:1023081624133

PubMed Abstract | CrossRef Full Text | Google Scholar

Skoda, A. M., Simovic, D., Karin, V., Kardum, V., Vranic, S., and Serman, L. (2018). The Role of the Hedgehog Signaling Pathway in Cancer: A Comprehensive Review. Bosn. J. Basic Med. Sci. 18, 8–20. doi:10.17305/bjbms.2018.2756

PubMed Abstract | CrossRef Full Text | Google Scholar

Soundararajan, R., Paranjape, A. N., Barsan, V., Chang, J. T., and Mani, S. A. (2015). A Novel Embryonic Plasticity Gene Signature that Predicts Metastatic Competence and Clinical Outcome. Sci. Rep. 5, 11766. doi:10.1038/srep11766

PubMed Abstract | CrossRef Full Text | Google Scholar

Speroni, L., Voutilainen, M., Mikkola, M. L., Klager, S. A., Schaeberle, C. M., Sonnenschein, C., et al. (2017). New Insights into Fetal Mammary Gland Morphogenesis: Differential Effects of Natural and Environmental Estrogens. Sci. Rep. 7, 40806. doi:10.1038/srep40806

PubMed Abstract | CrossRef Full Text | Google Scholar

Spike, B. T., Engle, D. D., Lin, J. C., Cheung, S. K., la, J., and Wahl, G. M. (2012). A Mammary Stem Cell Population Identified and Characterized in Late Embryogenesis Reveals Similarities to Human Breast Cancer. Cell. Stem Cell. 10, 183–197. doi:10.1016/j.stem.2011.12.018

PubMed Abstract | CrossRef Full Text | Google Scholar

Spina, E., and Cowin, P. (2021). Embryonic Mammary Gland Development. Semin. Cell. Dev. Biol. 114, 83–92. doi:10.1016/j.semcdb.2020.12.012

PubMed Abstract | CrossRef Full Text | Google Scholar

Stern, D. F. (2000). Tyrosine Kinase Signalling in Breast Cancer: ErbB Family Receptor Tyrosine Kinases. Breast Cancer Res. 2, 176–183. doi:10.1186/bcr51

PubMed Abstract | CrossRef Full Text | Google Scholar

Sun, M., Song, C. X., Huang, H., Frankenberger, C. A., Sankarasharma, D., Gomes, S., et al. (2013). HMGA2/TET1/HOXA9 Signaling Pathway Regulates Breast Cancer Growth and Metastasis. Proc. Natl. Acad. Sci. U. S. A. 110, 9920–9925. doi:10.1073/pnas.1305172110

PubMed Abstract | CrossRef Full Text | Google Scholar

Sundvall, M., Iljin, K., Kilpinen, S., Sara, H., Kallioniemi, O. P., and Elenius, K. (2008). Role of ErbB4 in Breast Cancer. J. Mammary Gland. Biol. Neoplasia 13, 259–268. doi:10.1007/s10911-008-9079-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Takebe, N., Warren, R. Q., and Ivy, S. P. (2011). Breast Cancer Growth and Metastasis: Interplay between Cancer Stem Cells, Embryonic Signaling Pathways and Epithelial-To-Mesenchymal Transition. Breast Cancer Res. 13, 211. doi:10.1186/bcr2876

PubMed Abstract | CrossRef Full Text | Google Scholar

Tanaka, H., Nakamura, M., Kameda, C., Kubo, M., Sato, N., Kuroki, S., et al. (2009). The Hedgehog Signaling Pathway Plays an Essential Role in Maintaining the CD44+CD24-/low Subpopulation and the Side Population of Breast Cancer Cells. Anticancer Res. 29, 2147–2157.

PubMed Abstract | Google Scholar

Tang, Y., Olufemi, L., Wang, M. T., and Nie, D. (2008). Role of Rho GTPases in Breast Cancer. Front. Biosci. 13, 759–776. doi:10.2741/2718

PubMed Abstract | CrossRef Full Text | Google Scholar

Thong, T., Wang, Y., Brooks, M. D., Lee, C. T., Scott, C., Balzano, L., et al. (2020). Hybrid Stem Cell States: Insights into the Relationship between Mammary Development and Breast Cancer Using Single-Cell Transcriptomics. Front. Cell. Dev. Biol. 8, 288. doi:10.3389/fcell.2020.00288

PubMed Abstract | CrossRef Full Text | Google Scholar

Trejo, C. L., Luna, G., Dravis, C., Spike, B. T., and Wahl, G. M. (2017). Lgr5 Is a Marker for Fetal Mammary Stem Cells, but Is Not Essential for Stem Cell Activity or Tumorigenesis. NPJ Breast Cancer 3, 16. doi:10.1038/s41523-017-0018-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Turner, N., and Grose, R. (2010). Fibroblast Growth Factor Signalling: from Development to Cancer. Nat. Rev. Cancer 10, 116–129. doi:10.1038/nrc2780

PubMed Abstract | CrossRef Full Text | Google Scholar

Vaage, J. (1984). Occurrence of Shared Nonviral Tumor-Associated Transplantation-type Antigens Among C3H/He Mammary Carcinomas. Cancer Res. 44, 1876–1879.

PubMed Abstract | Google Scholar

Van Amerongen, R., Bowman, A. N., and Nusse, R. (2012). Developmental Stage and Time Dictate the Fate of Wnt/beta-Catenin-Responsive Stem Cells in the Mammary Gland. Cell. Stem Cell. 11, 387–400. doi:10.1016/j.stem.2012.05.023

PubMed Abstract | CrossRef Full Text | Google Scholar

Van Der Auwera, I., van Laere, S. J., van Den Bosch, S. M., van Den Eynden, G. G., Trinh, B. X., van Dam, P. A., et al. (2008). Aberrant Methylation of the Adenomatous Polyposis Coli (APC) Gene Promoter Is Associated with the Inflammatory Breast Cancer Phenotype. Br. J. Cancer 99, 1735–1742. doi:10.1038/sj.bjc.6604705

PubMed Abstract | CrossRef Full Text | Google Scholar

Van Genderen, C., Okamura, R. M., Farinas, I., Quo, R. G., Parslow, T. G., Bruhn, L., et al. (1994). Development of Several Organs that Require Inductive Epithelial-Mesenchymal Interactions Is Impaired in LEF-1-Deficient Mice. Genes. Dev. 8, 2691–2703. doi:10.1101/gad.8.22.2691

PubMed Abstract | CrossRef Full Text | Google Scholar

Van Keymeulen, A., Lee, M. Y., Ousset, M., Brohee, S., Rorive, S., Giraddi, R. R., et al. (2015). Reactivation of Multipotency by Oncogenic PIK3CA Induces Breast Tumour Heterogeneity. Nature 525, 119–123. doi:10.1038/nature14665

PubMed Abstract | CrossRef Full Text | Google Scholar

Van Keymeulen, A., Rocha, A. S., Ousset, M., Beck, B., Bouvencourt, G., Rock, J., et al. (2011). Distinct Stem Cells Contribute to Mammary Gland Development and Maintenance. Nature 479, 189–193. doi:10.1038/nature10573

PubMed Abstract | CrossRef Full Text | Google Scholar

Vella, V., de Francesco, E. M., Lappano, R., Muoio, M. G., Manzella, L., Maggiolini, M., et al. (2020). Microenvironmental Determinants of Breast Cancer Metastasis: Focus on the Crucial Interplay between Estrogen and Insulin/Insulin-like Growth Factor Signaling. Front. Cell. Dev. Biol. 8, 608412. doi:10.3389/fcell.2020.608412

PubMed Abstract | CrossRef Full Text | Google Scholar

Veltmaat, J. M., Mailleux, A. A., Thiery, J. P., and Bellusci, S. (2003). Mouse Embryonic Mammogenesis as a Model for the Molecular Regulation of Pattern Formation. Differentiation 71, 1–17. doi:10.1046/j.1432-0436.2003.700601.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Veltmaat, J. M. (2017). Prenatal Mammary Gland Development in the Mouse: Research Models and Techniques for its Study from Past to Present. Methods Mol. Biol. 1501, 21–76. doi:10.1007/978-1-4939-6475-8_2

PubMed Abstract | CrossRef Full Text | Google Scholar

Veltmaat, J. M., Ramsdell, A. F., and Sterneck, E. (2013). Positional Variations in Mammary Gland Development and Cancer. J. Mammary Gland. Biol. Neoplasia 18, 179–188. doi:10.1007/s10911-013-9287-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Veltmaat, J. M., Relaix, F., le, L. T., Kratochwil, K., Sala, F. G., van Veelen, W., et al. (2006). Gli3-mediated Somitic Fgf10 Expression Gradients Are Required for the Induction and Patterning of Mammary Epithelium along the Embryonic Axes. Development 133, 2325–2335. doi:10.1242/dev.02394

PubMed Abstract | CrossRef Full Text | Google Scholar

Vial, J., Royet, A., Cassier, P., Tortereau, A., Dinvaut, S., Maillet, D., et al. (2019). The Ectodysplasin Receptor EDAR Acts as a Tumor Suppressor in Melanoma by Conditionally Inducing Cell Death. Cell. Death Differ. 26, 443–454. doi:10.1038/s41418-018-0128-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Voutilainen, M., Lindfors, P. H., Lefebvre, S., Ahtiainen, L., Fliniaux, I., Rysti, E., et al. (2012). Ectodysplasin Regulates Hormone-independent Mammary Ductal Morphogenesis via NF-kappaB. Proc. Natl. Acad. Sci. U. S. A. 109, 5744–5749. doi:10.1073/pnas.1110627109

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, B., Lindley, L. E., Fernandez-Vega, V., Rieger, M. E., Sims, A. H., and Briegel, K. J. (2012). The T Box Transcription Factor TBX2 Promotes Epithelial-Mesenchymal Transition and Invasion of Normal and Malignant Breast Epithelial Cells. PLoS One 7, e41355. doi:10.1371/journal.pone.0041355

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, D., Cai, C., Dong, X., Yu, Q. C., Zhang, X. O., Yang, L., et al. (2015). Identification of Multipotent Mammary Stem Cells by Protein C Receptor Expression. Nature 517, 81–84. doi:10.1038/nature13851

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, R. N., Green, J., Wang, Z., Deng, Y., Qiao, M., Peabody, M., et al. (2014). Bone Morphogenetic Protein (BMP) Signaling in Development and Human Diseases. Genes. Dis. 1, 87–105. doi:10.1016/j.gendis.2014.07.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, Y., Mcmahon, A. P., and Allen, B. L. (2007). Shifting Paradigms in Hedgehog Signaling. Curr. Opin. Cell. Biol. 19, 159–165. doi:10.1016/j.ceb.2007.02.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Wansleben, S., Peres, J., Hare, S., Goding, C. R., and Prince, S. (2014). T-box Transcription Factors in Cancer Biology. Biochim. Biophys. Acta 1846, 380–391. doi:10.1016/j.bbcan.2014.08.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Wen, X., Wu, Y., Awadasseid, A., Tanaka, Y., and Zhang, W. (2020). New Advances in Canonical Wnt/beta-Catenin Signaling in Cancer. Cancer Manag. Res. 12, 6987–6998. doi:10.2147/cmar.s258645

PubMed Abstract | CrossRef Full Text | Google Scholar

Wiedlocha, A., Haugsten, E. M., and Zakrzewska, M. (2021). Cells, 10. doi:10.3390/cells10092231Roles of the FGF-FGFR Signaling System in Cancer Development and Inflammation

CrossRef Full Text | Google Scholar

Williams, R., Jobling, S., Sims, A. H., Mou, C., Wilkinson, L., Collu, G. M., et al. (2022). Elevated EDAR Signalling Promotes Mammary Gland Tumourigenesis with Squamous Metaplasia. Oncogene 41, 1040–1049. doi:10.1038/s41388-021-01902-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Wilson, V., and Conlon, F. L. (2002). The T-Box Family. Genome Biol. 3, REVIEWS3008. doi:10.1186/gb-2002-3-6-reviews3008

PubMed Abstract | CrossRef Full Text | Google Scholar

Wu, X., Chen, H., Parker, B., Rubin, E., Zhu, T., Lee, J. S., et al. (2006). HOXB7, a Homeodomain Protein, Is Overexpressed in Breast Cancer and Confers Epithelial-Mesenchymal Transition. Cancer Res. 66, 9527–9534. doi:10.1158/0008-5472.can-05-4470

PubMed Abstract | CrossRef Full Text | Google Scholar

Wuidart, A., Ousset, M., Rulands, S., Simons, B. D., van Keymeulen, A., and Blanpain, C. (2016). Quantitative Lineage Tracing Strategies to Resolve Multipotency in Tissue-specific Stem Cells. Genes. Dev. 30, 1261–1277. doi:10.1101/gad.280057.116

PubMed Abstract | CrossRef Full Text | Google Scholar

Wuidart, A., Sifrim, A., Fioramonti, M., Matsumura, S., Brisebarre, A., Brown, D., et al. (2018). Early Lineage Segregation of Multipotent Embryonic Mammary Gland Progenitors. Nat. Cell. Biol. 20, 666–676. doi:10.1038/s41556-018-0095-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Wysolmerski, J. J., Philbrick, W. M., Dunbar, M. E., Lanske, B., Kronenberg, H., and Broadus, A. E. (1998). Rescue of the Parathyroid Hormone-Related Protein Knockout Mouse Demonstrates that Parathyroid Hormone-Related Protein Is Essential for Mammary Gland Development. Development 125, 1285–1294. doi:10.1242/dev.125.7.1285

PubMed Abstract | CrossRef Full Text | Google Scholar

Xu, X., Zhang, M., Xu, F., and Jiang, S. (2020). Wnt Signaling in Breast Cancer: Biological Mechanisms, Challenges and Opportunities. Mol. Cancer 19, 165. doi:10.1186/s12943-020-01276-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Yan, W., Cao, Q. J., Arenas, R. B., Bentley, B., and Shao, R. (2010). GATA3 Inhibits Breast Cancer Metastasis through the Reversal of Epithelial-Mesenchymal Transition. J. Biol. Chem. 285, 14042–14051. doi:10.1074/jbc.m110.105262

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, A., Schweitzer, R., Sun, D., Kaghad, M., Walker, N., Bronson, R. T., et al. (1999). p63 Is Essential for Regenerative Proliferation in Limb, Craniofacial and Epithelial Development. Nature 398, 714–718. doi:10.1038/19539

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, Y., Chan, J. Y., Temiz, N. A., and Yee, D. (2018). Insulin Receptor Substrate Suppression by the Tyrphostin NT157 Inhibits Responses to Insulin-like Growth Factor-I and Insulin in Breast Cancer Cells. Horm. Cancer 9, 371–382. doi:10.1007/s12672-018-0343-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Yarosh, W., Barrientos, T., Esmailpour, T., Lin, L., Carpenter, P. M., Osann, K., et al. (2008). TBX3 Is Overexpressed in Breast Cancer and Represses P14 ARF by Interacting with Histone Deacetylases. Cancer Res. 68, 693–699. doi:10.1158/0008-5472.can-07-5012

PubMed Abstract | CrossRef Full Text | Google Scholar

Yoh, K. E., Regunath, K., Guzman, A., Lee, S. M., Pfister, N. T., Akanni, O., et al. (2016). Repression of P63 and Induction of EMT by Mutant Ras in Mammary Epithelial Cells. Proc. Natl. Acad. Sci. U. S. A. 113, E6107–E6116. doi:10.1073/pnas.1613417113

PubMed Abstract | CrossRef Full Text | Google Scholar

Zabkiewicz, C., Resaul, J., Hargest, R., Jiang, W. G., and Ye, L. (2017). Bone Morphogenetic Proteins, Breast Cancer, and Bone Metastases: Striking the Right Balance. Endocr. Relat. Cancer 24, R349–R366. doi:10.1530/erc-17-0139

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhan, T., Rindtorff, N., and Boutros, M. (2017). Wnt Signaling in Cancer. Oncogene 36, 1461–1473. doi:10.1038/onc.2016.304

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, D., Sliwkowski, M. X., Mark, M., Frantz, G., Akita, R., Sun, Y., et al. (1997). Neuregulin-3 (NRG3): a Novel Neural Tissue-Enriched Protein that Binds and Activates ErbB4. Proc. Natl. Acad. Sci. U. S. A. 94, 9562–9567. doi:10.1073/pnas.94.18.9562

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhu, R., Gires, O., Zhu, L., Liu, J., Li, J., Yang, H., et al. (2019). TSPAN8 Promotes Cancer Cell Stemness via Activation of Sonic Hedgehog Signaling. Nat. Commun. 10, 2863. doi:10.1038/s41467-019-10739-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Zvelebil, M., Oliemuller, E., Gao, Q., Wansbury, O., Mackay, A., Kendrick, H., et al. (2013). Embryonic Mammary Signature Subsets Are Activated in Brca1-/- and Basal-like Breast Cancers. Breast Cancer Res. 15, R25. doi:10.1186/bcr3403

PubMed Abstract | CrossRef Full Text | Google Scholar

Glossary

Mammary rudiment (MR) The mammary gland during embryogenesis.

Placode Mammary rudiment at around E11.5 characterized by a multi-layered, disk-shaped morphology.

Genetically engineered mouse model (GEMM) Mouse (Mus musculus) with its genome modified using genetic engineering techniques. Genetic modifications may include gene loss-of-function (knockout, hypomorph) or gain-of-function (overexpression).

Sexual dimorphism Developmental process in the E14.5 mouse where development diverges in male and female.

Epithelial-to-mesenchymal transition (EMT) A process that enables cancer cells to suppress their epithelial features and take on mesenchymal ones. As a result, cells detach from their primary site and migrate to distal sites.

Cancer stem cell (CSC) Subpopulation of cancer cells with tumor-generating and multi-lineage differentiation potential. These cells are commonly associated with chemoresistance.

MMTV-PyMT (mouse mammary tumor virus-Polyoma virus middle T antigen) Mouse mammary gland carcinoma model, which is characterized by rapid development of multifocal tumors and extensive lung metastasis.

Lineage tracing A method to study the progenies of an initial population of cells that are marked genetically by a promoter-specific-Cre construct in combination with a reporter gene such as GFP.

Keywords: mammary gland, embryonic development, breast cancer, metastasis, molecular signatures

Citation: Lee MY (2022) Embryonic Programs in Cancer and Metastasis—Insights From the Mammary Gland. Front. Cell Dev. Biol. 10:938625. doi: 10.3389/fcell.2022.938625

Received: 07 May 2022; Accepted: 07 June 2022;
Published: 29 June 2022.

Edited by:

Philipp Kaldis, Lund University, Sweden

Reviewed by:

Prasanna Kolatkar, Qatar Biomedical Research Institute, Qatar
Nai Yang Fu, Duke-NUS Medical School, Singapore

Copyright © 2022 Lee. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: May Yin Lee, leemy@gis.a-star.edu.sg

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