Cornelia Brunner
Annika C. Betzler
Jennifer R. Brown
Amy H. Andreotti
Emanuela Grassilli- 1Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Ulm, Germany
- 2Chronic Lymphocytic Leukemia Center, Division of Medical Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, United States
- 3Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, United States
- 4Laboratory of Molecular Medicine, Department of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy
Editorial on the Research Topic
Targeting Bruton Tyrosine Kinase
The discovery of the Bruton tyrosine kinase (BTK) as the product of the defective gene in X-linked agammaglobulinemia (XLA) dates to almost 30 years ago (Tsukada et al., 1993). However, the more we study BTK, the more facets we discover in a protein once believed to be one size-fits-all and hematopoietic-specific. In this Research Topic, we present reviews and primary research providing insights into the complex role of BTK and an update on the latest findings regarding BTK as therapeutic target in inflammation, autoimmunity and malignancy.
Shortly after its discovery, BTK was positioned in the signal transduction pathway downstream of the B cell antigen receptor (BCR) and therefore relevant to adaptive immunity (Woyach et al., 2012). Upon BCR engagement, proteins including BTK, VAV, BLNK, PLC-γ2, and PI3K interact to form one signaling complex termed the signalosome (Seda and Mraz, 2015). Since BTK and VAV proteins are both components of the signalosome, Betzler et al. investigated whether these molecules lie in the same or independent pathways. Their data suggest a BTK-independent role for VAV1, but a BTK-dependent role for VAV3. Thus, these findings indicate that within the signalosome different VAV-family members can coordinate BCR-mediated signaling in different ways—necessary for BTK-dependent as well as independent signal transduction.
During the years it became evident that BTK is pivotal for transducing activation signals through receptors other than the BCR, such as CD19, BAFFR, TLRs, and chemokine receptors (Satterthwaite et al., 2000; Shinners et al., 2007; McDonald et al., 2021). In addition, BTK engagement in macrophages, myeloid cells, and mast cell activation revealed a role in the innate immune system. Recently, it was discovered that BTK regulates the NLRP3 inflammasome, involved in several acute and chronic disorders such as myocardial infarction, stroke, liver inflammation, type 2 diabetes, Alzheimer’s disease, Parkinson’s disease, and sepsis (Liu et al., 2017). The mini review by Weber addresses the role of BTK in NLRP3 inflammasome activation and highlights the resulting possibilities of targeting NLRP3-mediated inflammation using existing or future BTK inhibitors.
In the context of inflammatory and systemic autoimmune diseases, Neys et al. review the numerous studies supporting the potential of BTK targeting in conditions such as rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, atherosclerosis, and many others.
The discovery of dysregulation of BTK expression or activation in pathologies characterized by excessive B cell proliferation/activation—e g., B cell neoplasias and auto-immune disorders—led to the development of several specific BTK inhibitors (BTKis), a few of which are already approved for some B cell malignancies. In addition, numerous others BTKis are currently in clinical trials. The review by Estupiñán et al. provides a comprehensive overview about the currently available BTKis. They present ongoing clinical trials and discuss the unique properties of the different BTKis and their possible limitations arising from resistance mutations. Furthermore, they point out possible adverse effects of the BTKis and discuss their underlying mechanisms.
An important issue when targeting a kinase is understanding the detailed molecular mechanisms that control regulation. So far, the strategy has been to block the kinase domain via irreversible or reversible occupation of the ATP binding site. However, this approach can lead to off-target effects given that the targeted Cys residue is conserved in a small number of other kinases. The identification and/or synthesis of allosteric inhibitors may therefore allow an ever-increasing and more stringent specificity of next generation BTKis. The “hot topic” of understanding the role of interdomain interactions and their importance in regulating BTK activity is reviewed by Kueffer et al.
In the last decade it has been discovered that BTK does not occur in only one flavor and, most notably, that other isoforms seem to be primarily expressed in solid tumors, rather than in immune cells. p65BTK and p80BTK (BTK-C) were most recently identified as oncogenic isoforms in multiple solid tumors (Eifert et al., 2013; Kokabee et al., 2015; Grassilli et al., 2016; Giordano et al., 2019; Sala et al., 2019; Wang et al., 2016; Wang et al., 2017). The expression of these BTK isoforms in solid tumors is associated with tumor progression and poor prognosis, suggesting the use of BTKis not only in the treatment of B-cell malignancies and autoimmune or inflammatory disease, but also in solid tumors.
Grassilli et al. review the potential of p65BTK—an oncogenic isoform identified and characterized in colon carcinoma—as a novel biomarker and highlight its potential as a therapeutic target in several solid tumors. To complete the picture, Wang et al. review the newly described oncogenic BTK isoforms and further focus on the BTK-C isoform and its therapeutic potential in epithelial tumors. Complementarily, the perspective article by Uckun and Venkatachalam underlines the potential of BTK as a molecular target for the treatment of B cell malignancies and solid tumors. Moreover, they discuss the role of BTK in the tumor microenvironment and the current knowledge of coumarins as possible BTKis.
In conclusion, this Research Topic summarizes and discusses how recent research has expanded our understanding of BTK activation in several different cell types and has led to the identification of novel isoforms. Notably, the understanding of BTK’s new roles, such as in inflammation and solid tumors, will create opportunities to re-purpose BTKis for treating an increasing number of diseases. In particular, experimental evidence supporting the addition of BTKis to bypass tumor resistance to chemo- and targeted therapy is very promising. To better repurpose the BTKis, an essential challenge is to define the biological functions of each isoform and identify the relevant signaling pathways in which they are involved. Finally, given the off-target effects of several BTKis - all targeting the kinase’s ATP-binding domain—and the onset of drug resistance, significant efforts to develop ever more specific inhibitors are quite valuable. It is anticipated that understanding the molecular mechanism(s) that regulate and fine-tune kinase activity will allow the development of allosteric inhibitors to overcome these problems, thus paving the way for BTKis to take center stage in the era of personalized medicine.
Author Contributions
All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: BTK, innate and adaptive immune response, inflammation, autoimmune diseases, B-cell neoplasia, solid tumors
Citation: Brunner C, Betzler AC, Brown JR, Andreotti AH and Grassilli E (2022) Editorial: Targeting Bruton Tyrosine Kinase. Front. Cell Dev. Biol. 10:909655. doi: 10.3389/fcell.2022.909655
Received: 31 March 2022; Accepted: 08 April 2022;
Published: 26 April 2022.
Edited and reviewed by:
Zhi-Gang Zhang, Shanghai Jiao Tong University, ChinaCopyright © 2022 Brunner, Betzler, Brown, Andreotti and Grassilli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Cornelia Brunner, Y29ybmVsaWEuYnJ1bm5lckB1bmlrbGluaWstdWxtLmRl; Emanuela Grassilli, ZW1hbnVlbGEuZ3Jhc3NpbGxpQHVuaW1pYi5pdA==