Corrigendum: MFG-E8 maintains cellular homeostasis by suppressing endoplasmic reticulum stress in pancreatic exocrine acinar cells

A Corrigendum on
MFG-E8 maintains cellular homeostasis by suppressing endoplasmic reticulum stress in pancreatic exocrine acinar cells

by Ren Y, Liu W, Zhang J, Bi J, Fan M, Lv Y, Wu Z, Zhang Y and Wu R (2022). Front. Cell Dev. Biol. 9:803876. doi: 10.3389/fcell.2021.803876

In the original article, there was a mistake in Figures 2, 3 as published. The TEM results in the Sham group in Figure 2A inadvertently used the Sham group of mfge8-KO mice. In Figure 3A, the image of MPO with Vehicle treatment was misused from pancreas in CD11b group. The corrected Figures 2, 3 and its caption appear below:

FIGURE 2

FIGURE 2. Exogenous MFG-E8 alleviates pancreatic ER stress in vivo. In mice, arginine-AP stress was induced by 2 h intraperitoneal injections of 4.0 g/kg L-arginine. At 2 h after the last injection of L-arginine, normal saline (vehicle) or 20 μg/kg MFG-E8 were administered through intraperitoneal injection. The animals were sacrificed at 69 h after MFG-E8 treatment (i.e., 72 h after the first injection of L-arginine). Blood and tissue samples were collected. (A) Ultrastructural alterations in the pancreas (Transmission electron microscopy); (B,C) Representative photos of GRP78 staining and quantitative of GRP78 staining; (D) Western blot analysis of the expression of GRP78, phospho-IRE1α and IRE1α in the pancreas; (E) Western blot analysis of the expression of phospho-PERK, PERK, phospho-eIF2α and eIF2α in the pancreas; (F) Western blot analysis of the expression of nucl-ATF-6, cyto-ATF-6, nucl-H3 and cyto-β-actin in the pancreas; (G) Western blot analysis of the expression of CHOP, caspase-9 and cleaved caspase-9 in the pancreas. n = 4–6/group, error bars indicate the SEM; ∗ p < .05 versus Sham group; #p < .05 versus Vehicle group. MFG-E8, milk fat globule EGF factor 8; AP, acute pancreatitis; GRP78, glucose-regulated protein 78; eIF2α, eukaryotic initiation factor 2α; ATF-6, Activating Transcription Factor 6; CHOP, C/EBP homologous protein; Nucl, nucleus; Cyto, cytoplasm; H-3, histone-3; PERK, PKR-like endoplasmic reticulum kinase.

FIGURE 3

FIGURE 3. MFG-E8 alleviates the inflammatory response in experimental-AP through NF-κB signaling pathway. In mice, arginine-AP stress was induced by 2 h intraperitoneal injections of 4.0 g/kg L-arginine. At 2 h after the last injection of L-arginine, normal saline (vehicle) or 20 μg/kg MFG-E8 were administered through intraperitoneal injection. The animals were sacrificed at 69 h after MFG-E8 treatment (i.e., 72 h after the first injection of L-arginine). Blood and tissue samples were collected. (A) Representative photos of F4/80, CD11b, Gr1 and MPO staining; (B) Quantitative of F4/80, CD11b, Gr1 and MPO staining; (C) Serum TNF-α, IL-6 and HMGB-1 levels; (D,E) Western blot analysis of the expression of phospho-IκBα, IκBα, nucl-P65, cyto-P65, nucl-H3 and cyto-β-actin in the pancreas. n = 4–6/group, error bars indicate the SEM; ∗ p < .05 versus Sham group; #p < .05 versus Vehicle group. MFG-E8, milk fat globule EGF factor 8; AP, acute pancreatitis; MPO, myeloperoxidase; HMGB-1, High mobility group box 1; Nucl, nucleus; Cyto, cytoplasm; H-3, histone-3; IκBα, inhibitor of NF-κB-α; NF-κB p65, Nuclear Factor Kappa-B p65.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

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