Skip to main content

MINI REVIEW article

Front. Cell Dev. Biol., 24 October 2022
Sec. Cell Death and Survival
This article is part of the Research Topic Regulation and Coordination of the Different DNA Damage Responses and their Role in Tissue Homeostasis Maintenance View all 6 articles

The sooner, the better: ROS, kinases and nutrients at the onset of the damage response in Drosophila

  • Department of Genetics, Microbiology and Statistics, Faculty of Biology, and Institute of Biomedicine of the University of Barcelona, University of Barcelona, Barcelona, Spain

One of the main topics in regeneration biology is the nature of the early signals that trigger the damage response. Recent advances in Drosophila point to the MAP3 kinase Ask1 as a molecular hub that integrates several signals at the onset of regeneration. It has been discovered that reactive oxygen species (ROS) produced in damaged imaginal discs and gut epithelia will activate the MAP3 kinase Ask1. Severely damaged and apoptotic cells produce an enormous amount of ROS, which ensures their elimination by activating Ask1 and in turn the pro-apoptotic function of JNK. However, this creates an oxidative stress environment with beneficial effects that is sensed by neighboring healthy cells. This environment, in addition to the Pi3K/Akt nutrient sensing pathway, can be integrated into Ask1 to launch regeneration. Ultimately the activity of Ask1 depends on these and other inputs and modulates its signaling to achieve moderate levels of p38 and low JNK signaling and thus promote survival and regeneration. This model based on the dual function of Ask1 for early response to damage is discussed here.

Introduction

Regeneration, involving the repair or replacement of body parts, is widespread across the metazoans (Bely and Nyberg, 2010). The extent of regeneration varies among phyla, ranging from complete body parts to small pieces of damaged cells. Regardless of their regenerative capacity, different animal models, from worms to mammals, are being used in laboratories worldwide to shed some light on the basic principles of regeneration (Goldman and Poss, 2020). Research using animal models has generated several breakthroughs and has accumulated a vast amount of knowledge about the factors necessary for wound healing, regenerative proliferation, and re-patterning (Reuter et al., 2019). A definition of regeneration reminiscent of T.H. Morgan (Morgan, 1901) entails two scenarios in metazoans: (a) reparative or traumatic regeneration and (b) a physiological or homeostatic scenario, including cell turnover and tissue or epithelial lining wear and tear. In fact, optimal health is largely dependent upon tissue homeostasis, which involves cell replacement, regeneration, and tissue repair.

However, little is known about how tissues sense damage to initiate a cellular response. This aspect can be studied in model organisms where damage and its response can be reproducibly traced in small populations of cells and where genetic manipulation is possible, such as in Drosophila (Fox et al., 2020). How cells know when the regeneration machinery must be ignited is the subject of this paper, and in particular, I will focus on recent contributions that have unveiled the early dialog between damaged cells and healthy regenerating cells in Drosophila.

Drosophila imaginal discs are a model for tissue regeneration, as they undergo compensatory proliferation upon damage (Fan and Bergmann, 2008; Martin et al., 2008; Perez-Garijo et al., 2009; Smith-Bolton et al., 2009; Bergantiños et al., 2010; Herrera et al., 2013). Moreover, imaginal discs, like the mammalian liver, are epithelia that can heal and regenerate to their original size following removal of part of their mass, implying that some form of memory is retained in these organs (Hariharan and Serras, 2017). The wing imaginal discs, which are easily accessible, permit the manipulation and monitoring of both fast responses in the larval tissue and delayed responses in the adult tissue.

How is damage sensed?

Upon damage, the internal and external microenvironments can act as stressors for the cell. Stressors can affect the ionic balance of the cell membranes, change the permeability of the mitochondria and cause DNA damage. For example, injuries can produce intracellular Ca2+ release from the endoplasmic reticulum Ca2+ stores downstream of the inositol-3-phosphate receptor, which in turn requires gap junctions for intercellular propagation during wound healing (Razzell et al., 2013; Narciso et al., 2015; Restrepo and Basler, 2016). Also, ROS produced as byproducts of mitochondrial dysfunction generate oxidative stress that can also propagate to healthy neighbor cells (Serras, 2016). There are different sources of ROS in the cell. The evolutionary conserved membrane bound NAPH-oxidases (e.g. Duox, Nox) are specialized ROS producers and are involved in signaling (Vermot et al., 2021). ROS are also produced during mitochondrial electron transport or oxidation reactions. The free radical superoxide (O2), which predominates in the mitochondria, is the result of the reduction by one electron to oxygen. Two-electron reactions that reduce oxygen to hydrogen peroxide (H2O2) also occur in mitochondria (Finkel, 2011). ROS, which have generally been considered to be deleterious, are now emerging as active participants in cell signaling events (Finkel, 2011). For example, H2O2 is required for inflammatory cell recruitment (Niethammer et al., 2009; Moreira et al., 2010; Hunter et al., 2018; Niethammer, 2018). ROS produced after Xenopus tail or zebrafish fin lesions are required for regeneration (Gauron et al., 2013; Love et al., 2013; al Haj Baddar et al., 2019). ROS can rescue regeneration in Erk-deficient planarians (Jaenen et al., 2021). H2O2 is generated by the activation of NADPH oxidases (DUOX) and is important for rapid recruitment of pro-inflammatory cells to the wound (Razzell et al., 2013; Narciso et al., 2015). ROS and Ca++ production are interrelated as blocking the calcium waves or flashes produced after damage inhibits H2O2 release at the wound site, suggesting that the wound-induced Ca++ activates DUOX, likely via an EF-hand calcium-binding motif that, in turn, produces H2O2 (Razzell et al., 2013; Khan et al., 2017). Damaged cells can propagate H2O2 extracellularly and aquaporins might then act as conduits that are needed for these extracellular ROS to be channeled into the nearby healthy cells (Thiagarajah et al., 2017; Dhawan et al., 2021). Several lines of evidence show that interfering with Ca++ flashes or with oxidative stress alters wound healing and tissue repair (Razzell et al., 2013; Santabárbara-Ruiz et al., 2015; Restrepo and Basler, 2016; Brock et al., 2017; Khan et al., 2017). Therefore, these mechanisms operate as chemical alerts to sense damage and eventually activate regeneration.

In summary, the capacity to sense damage by Ca++ or ROS is a primary hallmark of tissue regeneration. ROS produced by various redox metabolic reactions have recently emerged as active participants in cell signaling events that spark regeneration.

JNK and p38: two MAP kinases that respond to ROS generated by cell damage

ROS act as second messengers to activate redox-sensitive signals, including the stress activated MAP kinases Jun-N Terminal kinase (JNK) and p38 (Droge, 2002; McCubrey et al., 2006; Jiang et al., 2011; Sato et al., 2014). In mammals, apoptosis enhances the activation of p38, resulting in Wnt3 transcription, a signal required for regeneration (Ankawa et al., 2021). In Drosophila, p38 and JNK signaling pathways respond to ROS and foster regeneration (Matsuzawa et al., 2005; Shi et al., 2014; Santabárbara-Ruiz et al., 2015; Verghese and Su, 2016; Brock et al., 2017; Khan et al., 2017; Pérez et al., 2017; Diwanji and Bergmann, 2018; Worley et al., 2018; Patel et al., 2019; Evans et al., 2022).

Because imaginal discs do not show significant apoptosis, the ectopic activation of pro-apoptotic genes in discrete zones is a suitable technique for neatly testing tissue recovery (Smith-Bolton et al., 2009; Bergantiños et al., 2010). This approach is less laborious than traditional surgical methods and can, therefore, be incorporated into large-scale genetic studies. It is based on the expression of the yeast transcription factor Gal4 targeted at discrete zones of the epithelium using tissue specific regulatory elements and also at a UAS that drives the expression of pro-apoptotic genes. With these genetic tools, the binding of Gal4 to the UAS will result in apoptosis in discrete zones of the tissue. The activity of Gal4 can be inhibited by a temperature-sensitive allele of Gal80, a Gal4 repressor. Genetic ablation is achieved by a temperature shift (from 17°C to 30°C) for a period of several hours during third instar larval development (Figure 1A).

FIGURE 1
www.frontiersin.org

FIGURE 1. ROS-dependent responses in early regeneration. (A) Design for genetic induction of cell death (genetic ablation). Drosophila strains carrying tissue specific (enhancer/promoter) expression of Gal4 (E/P-Gal4) will allow the transcription of the pro-apoptotic gene cloned downstream of the UAS sequence when Gal80 is inhibited (at 29–30°C). However, at 18°C the temperature sensitive Gal80 is active and blocks Gal4 which results in regeneration of the apoptotic tissue. (B) Scheme of wing imaginal discs showing the activity of some of the early responding signals after apoptosis. After genetic ablation, apoptotic cells produce high levels of ROS (Red in the central disc), which spreads to cells surrounding the apoptotic domain (shaded orange circle); Apoptotic cells and few non-apoptotic cells nearby, show expression of the JNK reporter TRE-DsRed (dark red). The puckered phosphatase (puc in green) is transcribed downstream JNK mainly in cells that are nearby the apoptotic zone. P-Akt and P-p38 respond in a broader domain and both found in surrounding the dead domain. Apoptotic cells are outlined with a black line in the center of each disc or with a solid black background.

When apoptosis is induced in imaginal discs, stress factors stimulate the pro-apoptotic role of JNK, resulting in a loop that ensures apoptosis (Shlevkov and Morata, 2012; Wells and Johnston, 2012). However, in addition to the pro-apoptotic role of JNK, proliferative and developmental roles have been widely reported (Pinal et al., 2019). Reporters of JNK activity, such as TRE-DsRed (Chatterjee and Bohmann, 2012), have been detected in cells that undergo apoptosis, but also in cells adjacent to the apoptotic zone, (Santabárbara-Ruiz et al., 2015) (Figure 1B). The phosphatase puckered (puc) is expressed downstream of the JNK pathway and acts as a negative regulator of the pathway (McEwen and Peifer, 2005). Puc is found at the edge of injured or apoptotic zones (Bosch et al., 2005, 2008; Smith-Bolton et al., 2009; Bergantiños et al., 2010; Santabárbara-Ruiz et al., 2015) (Figure 1B). In addition, blocking ROS production or mutants in the JNK pathway prevent regeneration (Smith-Bolton et al., 2009; Bergantiños et al., 2010; Fogarty et al., 2016; Khan et al., 2017).

Another MAP kinase that controls cellular responses to stress is p38. Phospho-p38, a reporter of p38 signaling, accumulates considerably in the imaginal disc, particularly in cells that surround the apoptotic zone (Figure 1B). Chemical antioxidants and genetic ROS scavengers interfere with the activation of p38; and loss-of-function of p38 signaling results in impaired regeneration (Santabárbara-Ruiz et al., 2015). Interestingly, the domain of puc cells is restricted to a few cells at the edge of the apoptotic zone, whereas increased p38 activity is much more extensive (Figure 1B). This observation is reminiscent of the antagonism between p38 and JNK (Wagner and Nebreda, 2009). In Drosophila gut, the interaction between p38 and its major target MK2 ensures proper stress response by keeping JNK activity low to avoid apoptosis (Seisenbacher et al., 2011).

The link between ROS production and JNK or p38 activation

There are two crucial questions that need to be addressed to understand the onset of repair. First, how is the production of ROS linked to JNK and p38 activity? And second, as the two MAPKs can promote apoptosis, how do JNK and p38 avoid killing the cells and be essential for regeneration? Recent advances have shed some light on these issues.

A key molecule in both instances is the apoptosis signal regulating kinase-1 (ASK1). Among the MAP3 kinases that operate upstream from JNK and p38, ASK1 is particularly sensitive to oxidative stressors (Takeda et al., 2006; Shiizaki et al., 2013; Sakauchi et al., 2017). ASK1 is inhibited when bound to thioredoxin (TRX) at the N-terminal region and to 13-4-4 protein close to the C-terminus (Saitoh et al., 1998; Zhang et al., 1999). Upon oxidative stress, these inhibitory proteins dissociate from the Ask1 complex and, thereby, ASK1 oligomerizes, autophosphorylates, recruits partners and becomes active (Bunkoczi et al., 2007; Takeda et al., 2008; Obsil and Obsilova, 2017). A threonine-rich catalytic domain is important for ASK1 signaling and can trigger JNK- and p38-dependent apoptosis (Ichijo et al., 1997; Tobiume et al., 2001, 2002; Bunkoczi et al., 2007).

Little is known about the partners and mechanism of action of the Drosophila Ask1. Ask1 can induce apoptosis in a JNK-dependent manner (Kuranaga et al., 2002). But loss of function of Ask1 impairs regeneration, which indicates that it has functions other than those related to apoptosis (Patel et al., 2019; Santabárbara-Ruiz et al., 2019). The activation of p38 (phospho-p38) mainly occurs in non-apoptotic cells and is Ask1 dependent (Patel et al., 2019; Santabárbara-Ruiz et al., 2019).

In addition to the autophosphorylation of Thr-rich sites, the phosphorylation of various Ser residues by other kinases also regulates mammalian ASK1 signaling activity (Zhang et al., 1999, 2005; Kim et al., 2001; Fujii et al., 2004). Interestingly, phosphorylation of human ASK1 Ser83 by Akt is thought to attenuate Ask1 activity and inhibit apoptosis (Kim et al., 2001; Zhang et al., 2005). Drosophila lacks the vertebrate N-terminal region that includes Ser. However, position 83 of Drosophila Ask1 is occupied by another Ser residue (Ser174 in human ASK1) embedded in a consensus that is highly conserved from sponges to mammals and that can be phosphorylated by Akt (Santabárbara-Ruiz et al., 2019). Amino-acid substitution of this Ser83/174 impedes p38 phosphorylation and regeneration, suggesting that at least in flies, Ask1 Ser83/174 is key for survival, skipping apoptosis and promoting repair (Santabárbara-Ruiz et al., 2019). Remarkably, healthy cells near apoptotic cells not only show increased phosphorylation of p38 but also a ROS-dependent increase in phosphorylated Akt, also known as Protein Kinase B (PKB) (Santabárbara-Ruiz et al., 2019) (Figure Fig1B). Thus, ROS have a dual function, one to activate Ask1 by removing Trx and the other to promote the activity of Akt. The Akt pathway can be enhanced through inhibition of the counteracting PTEN phosphatase. Interestingly, hydrogen peroxide oxidizes and inactivates human PTEN through disulfide bond formation between the catalytic domain Cys-124 and Cys-71 residues (Lee et al., 2002).

In summary, phosphorylation of Ask1 by Akt promotes survival, perhaps by keeping JNK at low levels and thus avoiding JNK-induced apoptosis, and by producing moderate levels of phospho-p38. These low JNK and moderate p38 levels set the scene for triggering tissue repair in healthy cells and avoiding apoptosis. Moreover, Akt-dependent phosphorylation of the Ser83/174 residue is required for p38-activity and for low JNK activity. Thus, this Ser83/174 residue must be key for swapping between p38 or JNK, and therefore between survival or apoptosis.

The adult Drosophila gut, a stem cell-based regenerating tissue, has many structural similarities to the human intestine and is the subject of intensive research that has been reviewed elsewhere (Colombani and Andersen, 2020; Zhang and Edgar, 2022). Remarkably, p38 is activated in enterocytes upon damage, and Ask1 acts upstream from p38 in response to numerous stressors, among them ROS produced by the NAPH oxidase NOX. This NOX/Ask1/P38 module in enterocytes acts as the signal for Upd cytokines to induce intestinal stem cells to proliferate, repair damage and regenerate the gut (Patel et al., 2019). Moreover, p38 signaling protects enterocytes from JNK-induced apoptosis after chronic stress (Seisenbacher et al., 2011).

Kinases and nutrients

Akt mediates phosphatidylinositol 3-kinase (Pi3K) to modulate different signals that foster proliferation and survival and prevent apoptosis. One of the canonical signals upstream from Pi3K/Akt is the insulin/insulin-like growth factor signaling pathway (IIS) that, upon activation of its tyrosine-kinase receptor, PIP2 is converted to PIP3 by Pi3K (Revathidevi and Munirajan, 2019). PIP3 recruits Akt to the plasma membrane and binds to phosphoinositide-dependent kinase-1 (PDK1), which in turn phosphorylates Akt. The conserved IIS and the target of rapamycin (TOR) pathways have a central role in metabolic homeostasis, stress response, growth, and aging, in flies, worms and mammals (Partridge et al., 2011).

One of the central mechanisms that organisms use to sense nutrients and their own nutritional status is the highly evolutionarily conserved insulin/insulin-like growth factor signaling (IIS) and the target of rapamycin (TOR) pathways (Flatt and Partridge, 2018). The decreased activity of these pathways can improve health and delay aging (Johnson et al., 2013; Flatt and Partridge, 2018). However, reduced activity of the IIS and TOR pathways can impair wound healing in flies and mice (Squarize et al., 2010; Kakanj et al., 2016). Thus, it is conceivable that the mechanisms that sense the nutritional status of the organism are key in sensing damage and therefore in the onset of regeneration. Indeed, it has recently been found that nutrient restriction blocks regeneration in similar phenotypes such as downregulation of Pi3K/Akt or in the mutated Ser83 of Ask1 (Santabárbara-Ruiz et al., 2019; Esteban-Collado et al., 2021). More importantly, it has also been found that the anomalous regeneration resulting from nutrient restriction or from mutated Ask1 Ser83 can be rescued by ectopic activation of p38, but not of JNK (Esteban-Collado et al., 2021). These observations demonstrate that the function of Pi3K/Akt in regeneration is channelled through p38 rather than JNK.

Discussion

In the last two decades several types of Drosophila epithelia, from embryo to adult, have contributed to our understanding of the early signals that respond to damage in an organism. A central feature is Ask1, a kinase initially associated with promoting cell death that has now emerged as being pivotal in regeneration. We foresee Ask1 as a signaling hub that integrates early moderate Ca++/ROS signals and phospho-Akt survival signals to divert Ask1 towards the p38-regeneration response (Figure 2). In contrast, high ROS or pro-apoptotic signals will act on the Ask1 hub to steer it towards JNK-dependent apoptosis (Figure 2). In addition to ROS and IIS/Pi3K/Akt, the decision to survive or not may require other molecules interacting with Ask1. Various signaling proteins such as the TNF receptor associated factors (TRAF) can bind to Ask1 and modulate its function (Nishitoh et al., 1998; Matsuzawa et al., 2005; Fujino et al., 2007).

FIGURE 2
www.frontiersin.org

FIGURE 2. Different requirement of JNK and p38 in Drosophila wing disc cells during apoptosis and regeneration. Apoptotic stimulus produces ROS that promote the dissociation of the thioredoxin (Trx) from Ask1. As a consequence, the activity of the Ask1 kinase domain is enhanced and JNKK will be phosphorylated and eventually the cell will irreversibly enter into apoptosis (Cell committed to die). The adjacent healthy cells will be sensitive to propagated ROS from the apoptotic cell, albeit in much lower levels, and moderately activate Ask1 (Cell recruited to regenerate). Ask1 can activate JNK in low levels or transiently until puckered (puc) attenuates JNK. In addition, the JNK-p38 antagonism could also attenuate JNK in the regenerating cell. This is key to maintain the viability of the regenerating cell. Activation of the Pi3K/Akt by nutrients or insulin signaling will result in phosphorylation of the Ser residue of YH_GVRESF Ask1 consensus. This signal is crucial for the activation of p38 but not JNK. Akt is also sensitive to ROS, likely through PTEN inactivation (dotted line).

JNK has a variety of functions other than promoting apoptosis (Martin-Blanco et al., 1998, 2000; Zeitlinger and Bohmann, 1999; Pastor-Pareja et al., 2004; Muñoz-Descalzo et al., 2005; Gettings et al., 2010; Martín et al., 2017; Pinal et al., 2019). JNK’s function in early regeneration events will strongly depend on its levels or perhaps on how long these are sustained. In Drosophila discs, constitutive activation of JNK results in apoptosis (Igaki, 2009; Shlevkov and Morata, 2012). But in physical injuries, JNK is activated at the wound edges (Bosch et al., 2005, 2008; Lee et al., 2005; Mattila et al., 2005; Blanco et al., 2010; Diaz-Garcia and Baonza, 2013; Ahmed-de-Prado et al., 2018). Reporters of JNK (i.e. TRE-dsRed) are expressed earlier than the phosphatase puc (i.e. puc > GFP) (Santabárbara-Ruiz et al., 2015). In addition, the cell lineage of these puc positive cells has shown that most of the reconstructed disc derives from puc positive cells (Bosch et al., 2008). The presence of puc positive cells indicates that JNK has been activated and dampened down, likely to restrict JNK to beneficial levels.

An exciting suggestion would be that JNK responds rapidly to the first flashes of ROS or Ca++. But once the phospho-Akt diverts Ask1 towards the survival pathway, JNK activity is restricted to protecting the tissue from further damage. This is an attractive hypothesis as it implies that the onset of stress-induced repair has two phases: one more dependent on JNK and a second on p38. Further research will be needed to elucidate whether the activity of these kinases overlaps.

The transcriptional signature downstream of the damage response includes the program for regenerative proliferation and repatterning (Vizcaya-Molina et al., 2018; Harris et al., 2020; Worley et al., 2022) and the identification of the blastema signature (Worley et al., 2022). In addition, JNK and p38 MAP kinases target the unpaired (Upd) cytokines (the Drosophila members of the interleukin-6 family), which are capable of activating the JAK/STAT pathway for growth control (Pastor-Pareja et al., 2008; Jiang et al., 2009; Diaz-Garcia and Baonza, 2013; Katsuyama et al., 2015; Santabárbara-Ruiz et al., 2015; la Fortezza et al., 2016; Verghese and Su, 2016; Ahmed-de-Prado et al., 2018; Worley et al., 2018; Patel et al., 2019). Also, non-apoptotic JNK activity in regeneration is driven by, for example, Wg/Wnt signaling (Smith-Bolton et al., 2009; Harris et al., 2016; Gracia-Latorre et al., 2022), Hippo signaling (Grusche et al., 2011; Sun and Irvine, 2011; Repiso et al., 2013; Meserve and Duronio, 2015; Ruiz-Romero et al., 2015), Gadd45, involved in DNA repair (Camilleri-Robles et al., 2019), and Ets21c, a JNK-dependent transcription factor key for disc blastema cells (Worley et al., 2022). Further research will be necessary to clarify whether the genetic response to JNK and p38 differs. In addition, studies are needed to explore whether this mechanism of sensing damage has been conserved through evolution.

Author contributions

FS wrote the manuscript.

Funding

This work was funded by grant PGC 2018–099763-B-I00 and PID 2021-123300NB-I00 of the Ministry of Science and Innovation -Spanish State Research Agency.

Acknowledgments

I apologize to the researchers whose work has not been discussed here due to space constraints. The author wishes to thank José Esteban Collado for reading the manuscript.

Conflict of interest

The author declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

Ahmed-de-Prado, S., Diaz-Garcia, S., and Baonza, A. (2018). Amputation-induced reactive oxygen species signaling is required for axolotl tail regeneration. Dev. Dyn. 441, 31–41. doi:10.1016/j.ydbio.2018.05.021

CrossRef Full Text | Google Scholar

al Haj Baddar, N. W., Chithrala, A., and Voss, S. R. (2019). Amputation-induced reactive oxygen species signaling is required for axolotl tail regeneration. Dev. Dyn. 248, 189–196. doi:10.1002/DVDY.5

PubMed Abstract | CrossRef Full Text | Google Scholar

Ankawa, R., Goldberger, N., Yosefzon, Y., Koren, E., Yusupova, M., Rosner, D., et al. (2021). Apoptotic cells represent a dynamic stem cell niche governing proliferation and tissue regeneration. Dev. Cell 56, 1900–1916.e5. e5. doi:10.1016/j.devcel.2021.06.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Bely, A. E., and Nyberg, K. G. (2010). Evolution of animal regeneration: Re-emergence of a field. Trends Ecol. Evol. 25, 161–170. doi:10.1016/j.tree.2009.08.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Bergantiños, C., Corominas, M., and Serras, F. (2010). Cell death-induced regeneration in wing imaginal discs requires JNK signalling. Development 137, 1169–1179. doi:10.1242/dev.045559

PubMed Abstract | CrossRef Full Text | Google Scholar

Blanco, E., Ruiz-Romero, M., Beltran, S., Bosch, M., Punset, A., Serras, F., et al. (2010). Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. BMC Dev. Biol. 10, 94. doi:10.1186/1471-213X-10-94

PubMed Abstract | CrossRef Full Text | Google Scholar

Bosch, M., Baguñà, J., and Serras, F. (2008). Origin and proliferation of blastema cells during regeneration of Drosophila wing imaginal discs. Int. J. Dev. Biol. 52, 1043–1050. doi:10.1387/ijdb.082608mb

PubMed Abstract | CrossRef Full Text | Google Scholar

Bosch, M., Serras, F., Martín-Blanco, E., and Baguñà, J. (2005). JNK signaling pathway required for wound healing in regenerating Drosophila wing imaginal discs. Dev. Biol. 280, 73–86. doi:10.1016/j.ydbio.2005.01.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Brock, A. R., Seto, M., and Smith-Bolton, R. K. (2017). Cap-n-Collar promotes tissue regeneration by regulating ROS and JNK signaling in the Drosophila melanogaster wing imaginal disc. Genetics 206, 1505–1520. doi:10.1534/genetics.116.196832

PubMed Abstract | CrossRef Full Text | Google Scholar

Bunkoczi, G., Salah, E., Filippakopoulos, P., Fedorov, O., Müller, S., Sobott, F., et al. (2007). Structural and functional characterization of the human protein kinase ASK1. Structure 15, 1215–1226. doi:10.1016/J.STR.2007.08.011

PubMed Abstract | CrossRef Full Text | Google Scholar

Camilleri-Robles, C., Serras, F., and Corominas, M. (2019). Role of D-GADD45 in JNK-dependent apoptosis and regeneration in Drosophila. Genes (Basel) 10, E378. doi:10.3390/genes10050378

PubMed Abstract | CrossRef Full Text | Google Scholar

Chatterjee, N., and Bohmann, D. (2012). A versatile ΦC31 based reporter system for measuring AP-1 and Nrf2 signaling in Drosophila and in tissue culture. PLoS One 7, e34063. doi:10.1371/journal.pone.0034063

PubMed Abstract | CrossRef Full Text | Google Scholar

Colombani, J., and Andersen, D. S. (2020). The Drosophila gut: A gatekeeper and coordinator of organism fitness and physiology. Wiley Interdiscip. Rev. Dev. Biol. 9. doi:10.1002/WDEV.378

PubMed Abstract | CrossRef Full Text | Google Scholar

Dhawan, S., Myers, P., Bailey, D. M. D., Ostrovsky, A. D., Evers, J. F., and Landgraf, M. (2021). Reactive oxygen species mediate activity-regulated dendritic plasticity through NADPH oxidase and aquaporin regulation. Front. Cell. Neurosci. 15, 641802. doi:10.3389/fncel.2021.641802

PubMed Abstract | CrossRef Full Text | Google Scholar

Diaz-Garcia, S., and Baonza, A. (2013). Pattern reorganization occurs independently of cell division during Drosophila wing disc regeneration in situ. Proc. Natl. Acad. Sci. U. S. A. 110, 13032–13037. doi:10.1073/pnas.1220543110

PubMed Abstract | CrossRef Full Text | Google Scholar

Diwanji, N., and Bergmann, A. (2018). An unexpected friend − ROS in apoptosis-induced compensatory proliferation: Implications for regeneration and cancer. Semin. Cell Dev. Biol. 80, 74–82. doi:10.1016/j.semcdb.2017.07.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Droge, W. (2002). Free radicals in the physiological control of cell function. Physiol. Rev. 82, 47–95. doi:10.1152/physrev.00018.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

Esteban-Collado, J., Corominas, M., and Serras, F. (2021). Nutrition and PI3K/Akt signaling are required for p38-dependent regeneration. Dev. Camb. 148, dev197087. doi:10.1242/dev.197087

PubMed Abstract | CrossRef Full Text | Google Scholar

Evans, C. J., Ting, L., Girard, J., and Banerjee, U. (2022). Injury-induced inflammatory signaling and hematopoiesis in Drosophila. Proc. Natl. Acad. Sci. U. S. A. 119, e2119109119. doi:10.1073/pnas.2119109119

PubMed Abstract | CrossRef Full Text | Google Scholar

Fan, Y., and Bergmann, A. (2008). Distinct mechanisms of apoptosis-induced compensatory proliferation in proliferating and differentiating tissues in the Drosophila eye. Dev. Cell 14, 399–410. doi:10.1016/j.devcel.2008.01.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Finkel, T. (2011). Signal transduction by reactive oxygen species. J. Cell Biol. 194, 7–15. doi:10.1083/jcb.201102095

PubMed Abstract | CrossRef Full Text | Google Scholar

Flatt, T., and Partridge, L. (2018). Horizons in the evolution of aging. BMC Biol. 16, 93. doi:10.1186/S12915-018-0562-Z

PubMed Abstract | CrossRef Full Text | Google Scholar

Fogarty, C. E., Diwanji, N., Lindblad, J. L., Tare, M., Amcheslavsky, A., Makhijani, K., et al. (2016). Extracellular reactive oxygen species drive apoptosis-induced proliferation via Drosophila macrophages. Curr. Biol. 26, 575–584. doi:10.1016/j.cub.2015.12.064

PubMed Abstract | CrossRef Full Text | Google Scholar

Fox, D. T., Cohen, E., and Smith-Bolton, R. (2020). Model systems for regeneration: Drosophila. Development 147, dev173781. doi:10.1242/dev.173781

PubMed Abstract | CrossRef Full Text | Google Scholar

Fujii, K., Goldman, E. H., Park, H. R., Zhang, L., Chen, J., and Fu, H. (2004). Negative control of apoptosis signal-regulating kinase 1 through phosphorylation of Ser-1034. Oncogene 23, 5099–5104. doi:10.1038/SJ.ONC.1207668

PubMed Abstract | CrossRef Full Text | Google Scholar

Fujino, G., Noguchi, T., Matsuzawa, A., Yamauchi, S., Saitoh, M., Takeda, K., et al. (2007). Thioredoxin and TRAF family proteins regulate reactive oxygen species-dependent activation of ASK1 through reciprocal modulation of the N-terminal homophilic interaction of ASK1. Mol. Cell. Biol. 27, 8152–8163. doi:10.1128/MCB.00227-07

PubMed Abstract | CrossRef Full Text | Google Scholar

Gauron, C., Rampon, C., Bouzaffour, M., Ipendey, E., Teillon, J., Volovitch, M., et al. (2013). Sustained production of ROS triggers compensatory proliferation and is required for regeneration to proceed. Sci. Rep. 3, 2084. doi:10.1038/srep02084

PubMed Abstract | CrossRef Full Text | Google Scholar

Gettings, M., Serman, F., Rousset, R., Bagnerini, P., Almeida, L., and Noselli, S. (2010). JNK signalling controls remodelling of the segment boundary through cell reprogramming during Drosophila morphogenesis. PLoS Biol. 8, e1000390. doi:10.1371/journal.pbio.1000390

PubMed Abstract | CrossRef Full Text | Google Scholar

Goldman, J. A., and Poss, K. D. (2020). Gene regulatory programmes of tissue regeneration. Nat. Rev. Genet. 21, 511–525. doi:10.1038/s41576-020-0239-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Gracia-Latorre, E., Pérez, L., Muzzopappa, M., and Milán, M. (2022). A single WNT enhancer drives specification and regeneration of the Drosophila wing. Nat. Commun. 13, 4794. doi:10.1038/s41467-022-32400-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Grusche, F. A., Degoutin, J. L., Richardson, H. E., and Harvey, K. F. (2011). The Salvador/Warts/Hippo pathway controls regenerative tissue growth in Drosophila melanogaster. Dev. Biol. 350, 255–266. doi:10.1016/j.ydbio.2010.11.020

PubMed Abstract | CrossRef Full Text | Google Scholar

Hariharan, I. K., and Serras, F. (2017). Imaginal disc regeneration takes flight. Curr. Opin. Cell Biol. 48, 10–16. doi:10.1016/j.ceb.2017.03.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Harris, R. E., Setiawan, L., Saul, J., and Hariharan, I. K. (2016). Localized epigenetic silencing of a damage-activated WNT enhancer limits regeneration in mature Drosophila imaginal discs. Elife 5, e11588. doi:10.7554/eLife.11588

PubMed Abstract | CrossRef Full Text | Google Scholar

Harris, R. E., Stinchfield, M. J., Nystrom, S. L., McKay, D. J., and Hariharan, I. K. (2020). Damage-responsive, maturity-silenced enhancers regulate multiple genes that direct regeneration in drosophila. Elife 9, e58305–e58326. doi:10.7554/eLife.58305

PubMed Abstract | CrossRef Full Text | Google Scholar

Herrera, S. C., Martin, R., and Morata, G. (2013). Tissue homeostasis in the wing disc of Drosophila melanogaster: Immediate response to massive damage during development. PLoS Genet. 9, e1003446. doi:10.1371/journal.pgen.100344610.1371/journal.pgen.1003446

PubMed Abstract | CrossRef Full Text | Google Scholar

Hunter, M. v., Willoughby, P. M., Bruce, A. E. E., and Fernandez-Gonzalez, R. (2018). Oxidative stress orchestrates cell polarity to promote embryonic wound healing. Dev. Cell 47, 377–387. e4. doi:10.1016/j.devcel.2018.10.013

PubMed Abstract | CrossRef Full Text | Google Scholar

Ichijo, H., Nishida, E., Irie, K., ten Dijke, P., Saitoh, M., Moriguchi, T., et al. (1997). Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275, 90–94. doi:10.1126/science.275.5296.90

PubMed Abstract | CrossRef Full Text | Google Scholar

Igaki, T. (2009). Correcting developmental errors by apoptosis: Lessons from Drosophila JNK signaling. Apoptosis 14, 1021–1028. doi:10.1007/s10495-009-0361-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Jaenen, V., Fraguas, S., Bijnens, K., Heleven, M., Artois, T., Romero, R., et al. (2021). Reactive oxygen species rescue regeneration after silencing the MAPK–ERK signaling pathway in Schmidtea mediterranea. Sci. Rep. 11, 881. doi:10.1038/s41598-020-79588-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Jiang, F., Zhang, Y., and Dusting, G. J. (2011). NADPH oxidase-mediated redox signaling: Roles in cellular stress response, stress tolerance, and tissue repair. Pharmacol. Rev. 63, 218–242. doi:10.1124/pr.110.002980

PubMed Abstract | CrossRef Full Text | Google Scholar

Jiang, H., Patel, P. H., Kohlmaier, A., Grenley, M. O., McEwen, D. G., and Edgar, B. A. (2009). Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell 137, 1343–1355. doi:10.1016/j.cell.2009.05.014

PubMed Abstract | CrossRef Full Text | Google Scholar

Johnson, S. C., Rabinovitch, P. S., and Kaeberlein, M. (2013). mTOR is a key modulator of ageing and age-related disease. Nature 493, 338–345. doi:10.1038/nature11861

PubMed Abstract | CrossRef Full Text | Google Scholar

Kakanj, P., Moussian, B., Grönke, S., Bustos, V., Eming, S. A., Partridge, L., et al. (2016). Insulin and TOR signal in parallel through FOXO and S6K to promote epithelial wound healing. Nat. Commun. 7, 12972. doi:10.1038/ncomms12972

PubMed Abstract | CrossRef Full Text | Google Scholar

Katsuyama, T., Comoglio, F., Seimiya, M., Cabuy, E., and Paro, R. (2015). During Drosophila disc regeneration, JAK/STAT coordinates cell proliferation with Dilp8-mediated developmental delay. Proc. Natl. Acad. Sci. U. S. A. 112, E2327–E2336. doi:10.1073/pnas.1423074112

PubMed Abstract | CrossRef Full Text | Google Scholar

Khan, S. J., Abidi, S. N. F., Skinner, A., Tian, Y., and Smith-Bolton, R. K. (2017). The Drosophila Duox maturation factor is a key component of a positive feedback loop that sustains regeneration signaling. PLoS Genet. 13, e1006937. doi:10.1371/journal.pgen.1006937

PubMed Abstract | CrossRef Full Text | Google Scholar

Kim, A. H., Khursigara, G., Sun, X., Franke, T. F., and Chao, M. v. (2001). Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1. Mol. Cell. Biol. 21, 893–901. doi:10.1128/MCB.21.3.893-901.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

Kuranaga, E., Kanuka, H., Igaki, T., Sawamoto, K., Ichijo, H., Okano, H., et al. (2002). Reaper-mediated inhibition of DIAP1-induced DTRAF1 degradation results in activation of JNK in Drosophila. Nat. Cell Biol. 4, 705–710. doi:10.1038/ncb842

PubMed Abstract | CrossRef Full Text | Google Scholar

la Fortezza, M., Schenk, M., Cosolo, A., Kolybaba, A., Grass, I., and Classen, A.-K. (2016). JAK/STAT signalling mediates cell survival in response to tissue stress. Development 143, 2907–2919. doi:10.1242/dev.132340

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, N., Maurange, C., Ringrose, L., and Paro, R. (2005). Suppression of Polycomb group proteins by JNK signalling induces transdetermination in Drosophila imaginal discs. Nature 438, 234–237. nature04120 [pii]. doi:10.1038/nature04120

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, S. R., Yang, K. S., Kwon, J., Lee, C., Jeong, W., and Rhee, S. G. (2002). Reversible inactivation of the tumor suppressor PTEN by H2O2. J. Biol. Chem. 277, 20336–20342. doi:10.1074/JBC.M111899200

PubMed Abstract | CrossRef Full Text | Google Scholar

Love, N. R., Chen, Y., Ishibashi, S., Kritsiligkou, P., Lea, R., Koh, Y., et al. (2013). Amputation-induced reactive oxygen species are required for successful Xenopus tadpole tail regeneration. Nat. Cell Biol. 15, 222–228. doi:10.1038/ncb2659

PubMed Abstract | CrossRef Full Text | Google Scholar

Martin, F. A., Perez-Garijo, A., and Morata, G. (2008). Apoptosis in Drosophila: Compensatory proliferation and undead cells. Int. J. Dev. Biol. 53, 1341–1347. 072447fm [pii]. doi:10.1387/ijdb.072447fm

PubMed Abstract | CrossRef Full Text | Google Scholar

Martín, R., Pinal, N., and Morata, G. (2017). Distinct regenerative potential of trunk and appendages of drosophila mediated by JNK signalling. Dev. Camb. 144, 3946–3956. doi:10.1242/dev.155507

CrossRef Full Text | Google Scholar

Martin-Blanco, E., Gampel, A., Ring, J., Virdee, K., Kirov, N., Tolkovsky, A. M., et al. (1998). Puckered encodes a phosphatase that mediates a feedback loop regulating JNK activity during dorsal closure in Drosophila. Genes Dev. 12, 557–570. doi:10.1101/gad.12.4.557

PubMed Abstract | CrossRef Full Text | Google Scholar

Martin-Blanco, E., Pastor-Pareja, J. C., and Garcia-Bellido, A. (2000). JNK and decapentaplegic signaling control adhesiveness and cytoskeleton dynamics during thorax closure in Drosophila. Proc. Natl. Acad. Sci. U. S. A. 97, 7888–7893. [pii]. doi:10.1073/pnas.97.14.7888

PubMed Abstract | CrossRef Full Text | Google Scholar

Matsuzawa, A., Saegusa, K., Noguchi, T., Sadamitsu, C., Nishitoh, H., Nagai, S., et al. (2005). ROS-dependent activation of the TRAF6-ASK1-p38 pathway is selectively required for TLR4-mediated innate immunity. Nat. Immunol. 6, 587–592. doi:10.1038/ni1200

PubMed Abstract | CrossRef Full Text | Google Scholar

Mattila, J., Omelyanchuk, L., Kyttala, S., Turunen, H., and Nokkala, S. (2005). Role of Jun N-terminal Kinase (JNK) signaling in the wound healing and regeneration of a Drosophila melanogaster wing imaginal disc. Int. J. Dev. Biol. 49, 391–399. doi:10.1387/ijdb.052006jm

PubMed Abstract | CrossRef Full Text | Google Scholar

McCubrey, J. A., Lahair, M. M., and Franklin, R. A. (2006). Reactive oxygen species-induced activation of the MAP kinase signaling pathways. Antioxid. Redox Signal. 8, 1775–1789. doi:10.1089/ars.2006.8.1775

PubMed Abstract | CrossRef Full Text | Google Scholar

McEwen, D. G., and Peifer, M. (2005). Puckered, a Drosophila MAPK phosphatase, ensures cell viability by antagonizing JNK-induced apoptosis. Development 132, 3935–3946. dev.01949 [pii]. doi:10.1242/dev.01949

PubMed Abstract | CrossRef Full Text | Google Scholar

Meserve, J. H., and Duronio, R. J. (2015). Scalloped and Yorkie are required for cell cycle re-entry of quiescent cells after tissue damage. Development 142, 2740–2751. doi:10.1242/dev.119339

PubMed Abstract | CrossRef Full Text | Google Scholar

Moreira, S., Stramer, B., Evans, I., Wood, W., and Martin, P. (2010). Prioritization of competing damage and developmental signals by migrating macrophages in the Drosophila embryo. Curr. Biol. 20, 464–470. doi:10.1016/j.cub.2010.01.047

PubMed Abstract | CrossRef Full Text | Google Scholar

Morgan, T. H. (1901). Regeneration. New York: The Macmillan Company. doi:10.5962/bhl.title.1114

CrossRef Full Text | Google Scholar

Muñoz-Descalzo, S., Terol, J., and Paricio, N. (2005). Cabut, a C2H2 zinc finger transcription factor, is required during Drosophila dorsal closure downstream of JNK signaling. Dev. Biol. 287, 168–179. doi:10.1016/j.ydbio.2005.08.048

PubMed Abstract | CrossRef Full Text | Google Scholar

Narciso, C., Wu, Q., Brodskiy, P., Garston, G., Baker, R., Fletcher, A., et al. (2015). Patterning of wound-induced intercellular Ca(2+) flashes in a developing epithelium. Phys. Biol. 12, 056005. doi:10.1088/1478-3975/12/5/056005

PubMed Abstract | CrossRef Full Text | Google Scholar

Niethammer, P., Grabher, C., Look, A. T., and Mitchison, T. J. (2009). A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish. Nature 459, 996–999. doi:10.1038/nature08119

PubMed Abstract | CrossRef Full Text | Google Scholar

Niethammer, P. (2018). Wound redox gradients revisited. Semin. Cell Dev. Biol. 80, 13–16. doi:10.1016/j.semcdb.2017.07.038

PubMed Abstract | CrossRef Full Text | Google Scholar

Nishitoh, H., Saitoh, M., Mochida, Y., Takeda, K., Nakano, H., Rothe, M., et al. (1998). ASK1 is essential for JNK/SAPK activation by TRAF2. Mol. Cell 2, 389–395. doi:10.1016/s1097-2765(00)80283-x

PubMed Abstract | CrossRef Full Text | Google Scholar

Obsil, T., and Obsilova, V. (2017). Structural aspects of protein kinase ASK1 regulation. Adv. Biol. Regul. 66, 31–36. doi:10.1016/J.JBIOR.2017.10.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Partridge, L., Alic, N., Bjedov, I., and Piper, M. D. W. (2011). Ageing in Drosophila: The role of the insulin/Igf and TOR signalling network. Exp. Gerontol. 46, 376–381. doi:10.1016/j.exger.2010.09.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Pastor-Pareja, J. C., Grawe, F., Martin-Blanco, E., and Garcia-Bellido, A. (2004). Invasive cell behavior during Drosophila imaginal disc eversion is mediated by the JNK signaling cascade. Dev. Cell 7, 387–399. [pii]. doi:10.1016/j.devcel.2004.07.022

PubMed Abstract | CrossRef Full Text | Google Scholar

Pastor-Pareja, J. C., Wu, M., and Xu, T. (2008). An innate immune response of blood cells to tumors and tissue damage in Drosophila. Dis. Model. Mech. 1, 144–154. doi:10.1242/dmm.000950

PubMed Abstract | CrossRef Full Text | Google Scholar

Patel, P. H., Pénalva, C., Kardorff, M., Roca, M., Pavlović, B., Thiel, A., et al. (2019). Damage sensing by a Nox-Ask1-MKK3-p38 signaling pathway mediates regeneration in the adult Drosophila midgut. Nat. Commun. 10, 4365. doi:10.1038/s41467-019-12336-w

PubMed Abstract | CrossRef Full Text | Google Scholar

Pérez, E., Lindblad, J. L., and Bergmann, A. (2017). Tumor-promoting function of apoptotic caspases by an amplification loop involving ROS, macrophages and JNK in Drosophila. Elife 6, e26747. doi:10.7554/eLife.26747

PubMed Abstract | CrossRef Full Text | Google Scholar

Perez-Garijo, A., Shlevkov, E., and Morata, G. (2009). The role of Dpp and Wg in compensatory proliferation and in the formation of hyperplastic overgrowths caused by apoptotic cells in the Drosophila wing disc. Development 136, 11691169–11691177. [pii]. doi:10.1242/dev.034017

PubMed Abstract | CrossRef Full Text | Google Scholar

Pinal, N., Calleja, M., and Morata, G. (2019). Pro-apoptotic and pro-proliferation functions of the JNK pathway of drosophila: Roles in cell competition, tumorigenesis and regeneration. Open Biol. 9, 180256. doi:10.1098/rsob.180256

PubMed Abstract | CrossRef Full Text | Google Scholar

Razzell, W., Evans, I. R., Martin, P., and Wood, W. (2013). Calcium flashes orchestrate the wound inflammatory response through DUOX activation and hydrogen peroxide release. Curr. Biol. 23, 424–429. doi:10.1016/j.cub.2013.01.058

PubMed Abstract | CrossRef Full Text | Google Scholar

Repiso, A., Bergantinos, C., Serras, F., Bergantiños, C., and Serras, F. (2013). Cell fate respecification and cell division orientation drive intercalary regeneration in Drosophila wing discs. Development 140, 3541–3551. doi:10.1242/dev.095760

PubMed Abstract | CrossRef Full Text | Google Scholar

Restrepo, S., and Basler, K. (2016). Drosophila wing imaginal discs respond to mechanical injury via slow InsP3R-mediated intercellular calcium waves. Nat. Commun. 7, 12450. doi:10.1038/ncomms12450

PubMed Abstract | CrossRef Full Text | Google Scholar

Reuter, H., Vogg, M. C., and Serras, F. (2019). Repair, regenerate and reconstruct: Meeting the state-of-the-art. Cambridge: Development. 146. doi:10.1242/dev.176974

PubMed Abstract | CrossRef Full Text | Google Scholar

Revathidevi, S., and Munirajan, A. K. (2019). Akt in cancer: Mediator and more. Semin. Cancer Biol. 59, 80–91. doi:10.1016/J.SEMCANCER.2019.06.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Ruiz-Romero, M., Blanco, E., Paricio, N., Serras, F., and Corominas, M. (2015). Cabut/dTIEG associates with the transcription factor Yorkie for growth control. EMBO Rep. 16, 362–369. doi:10.15252/embr.201439193

PubMed Abstract | CrossRef Full Text | Google Scholar

Saitoh, M., Nishitoh, H., Fujii, M., Takeda, K., Tobiume, K., Sawada, Y., et al. (1998). Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1. EMBO J. 17, 2596–2606. doi:10.1093/emboj/17.9.2596

PubMed Abstract | CrossRef Full Text | Google Scholar

Sakauchi, C., Wakatsuki, H., Ichijo, H., and Hattori, K. (2017). Pleiotropic properties of ASK1. Biochim. Biophys. Acta. Gen. Subj. 1861, 3030–3038. doi:10.1016/j.bbagen.2016.09.028

PubMed Abstract | CrossRef Full Text | Google Scholar

Santabárbara-Ruiz, P., Esteban-Collado, J., Pérez, L., Viola, G., Abril, J. F., Milán, M., et al. (2019). Ask1 and Akt act synergistically to promote ROS-dependent regeneration in Drosophila. PLoS Genet. 15, e1007926. doi:10.1371/journal.pgen.1007926

PubMed Abstract | CrossRef Full Text | Google Scholar

Santabárbara-Ruiz, P., López-Santillán, M., Martínez-Rodríguez, I., Binagui-Casas, A., Pérez, L., Milán, M., et al. (2015). ROS-induced JNK and p38 signaling is required for unpaired cytokine activation during Drosophila regeneration. PLoS Genet. 11, e1005595. doi:10.1371/journal.pgen.1005595

PubMed Abstract | CrossRef Full Text | Google Scholar

Sato, A., Okada, M., Shibuya, K., Watanabe, E., Seino, S., Narita, Y., et al. (2014). Pivotal role for ROS activation of p38 MAPK in the control of differentiation and tumor-initiating capacity of glioma-initiating cells. Stem Cell Res. 12, 119–131. doi:10.1016/j.scr.2013.09.012

PubMed Abstract | CrossRef Full Text | Google Scholar

Seisenbacher, G., Hafen, E., and Stocker, H. (2011). MK2-dependent p38b signalling protects Drosophila hindgut enterocytes against JNK-induced apoptosis under chronic stress. PLoS Genet. 7, e1002168. doi:10.1371/journal.pgen.1002168

PubMed Abstract | CrossRef Full Text | Google Scholar

Serras, F. (2016). The benefits of oxidative stress for tissue repair and regeneration. Fly. (Austin) 10, 128–133. doi:10.1080/19336934.2016.1188232

PubMed Abstract | CrossRef Full Text | Google Scholar

Shi, Y., Nikulenkov, F., Zawacka-Pankau, J., Li, H., Gabdoulline, R., Xu, J., et al. (2014). ROS-dependent activation of JNK converts p53 into an efficient inhibitor of oncogenes leading to robust apoptosis. Cell Death Differ. 21, 612–623. doi:10.1038/cdd.2013.186

PubMed Abstract | CrossRef Full Text | Google Scholar

Shiizaki, S., Naguro, I., and Ichijo, H. (2013). Activation mechanisms of ASK1 in response to various stresses and its significance in intracellular signaling. Adv. Biol. Regul. 53, 135–144. doi:10.1016/j.jbior.2012.09.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Shlevkov, E., and Morata, G. (2012). A dp53/JNK-dependant feedback amplification loop is essential for the apoptotic response to stress in Drosophila. Cell Death Differ. 19, 451–460. doi:10.1038/cdd.2011.113

PubMed Abstract | CrossRef Full Text | Google Scholar

Smith-Bolton, R. K., Worley, M. I., Kanda, H., and Hariharan, I. K. (2009). Regenerative growth in Drosophila imaginal discs is regulated by Wingless and Myc. Dev. Cell 16, 797–809. doi:10.1016/j.devcel.2009.04.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Squarize, C. H., Castilho, R. M., Bugge, T. H., and Gutkind, J. S. (2010). Accelerated wound healing by mTOR activation in genetically defined mouse models. PLoS One 5, e10643. doi:10.1371/journal.pone.0010643

PubMed Abstract | CrossRef Full Text | Google Scholar

Sun, G., and Irvine, K. D. (2011). Regulation of Hippo signaling by Jun kinase signaling during compensatory cell proliferation and regeneration, and in neoplastic tumors. Dev. Biol. 350, 139–151. doi:10.1016/j.ydbio.2010.11.036

PubMed Abstract | CrossRef Full Text | Google Scholar

Takeda, K., Noguchi, T., Naguro, I., and Ichijo, H. (2008). Apoptosis signal-regulating kinase 1 in stress and immune response. Annu. Rev. Pharmacol. Toxicol. 48, 199–225. doi:10.1146/annurev.pharmtox.48.113006.094606

PubMed Abstract | CrossRef Full Text | Google Scholar

Takeda, K., Shimozono, R., Noguchi, T., Umeda, T., Morimoto, Y., Naguro, I., et al. (2006). Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1. J. Biol. Chem. 282, 7522–7531. doi:10.1074/jbc.M607177200

PubMed Abstract | CrossRef Full Text | Google Scholar

Thiagarajah, J., Jeffrey, C., Goettel, J., Verkman, A., and Lencer, W. (2017). Aquaporin-3 mediates hydrogen peroxide-dependent responses to environmental stress in colonic epithelia. Proc. Natl. Acad. Sci. U. S. A. 114, 568–573. doi:10.1073/pnas.1612921114

PubMed Abstract | CrossRef Full Text | Google Scholar

Tobiume, K., Matsuzawa, A., Takahashi, T., Nishitoh, H., Morita, K., Takeda, K., et al. (2001). ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis. EMBO Rep. 2, 222–228. doi:10.1093/embo-reports/kve046

PubMed Abstract | CrossRef Full Text | Google Scholar

Tobiume, K., Saitoh, M., and Ichijo, H. (2002). Activation of apoptosis signal-regulating kinase 1 by the stress-induced activating phosphorylation of pre-formed oligomer. J. Cell. Physiol. 191, 95–104. doi:10.1002/jcp.10080

PubMed Abstract | CrossRef Full Text | Google Scholar

Verghese, S., and Su, T. T. (2016). Drosophila Wnt and STAT define apoptosis-resistant epithelial cells for tissue regeneration after irradiation. PLoS Biol. 14, e1002536. doi:10.1371/journal.pbio.1002536

PubMed Abstract | CrossRef Full Text | Google Scholar

Vermot, A., Petit-Härtlein, I., Smith, S. M. E., and Fieschi, F. (2021). NADPH oxidases (NOX): An overview from discovery, molecular mechanisms to physiology and pathology. Antioxidants 10, 890. doi:10.3390/ANTIOX10060890

PubMed Abstract | CrossRef Full Text | Google Scholar

Vizcaya-Molina, E., Klein, C. C., Serras, F., Mishra, R. K., Guigó, R., and Corominas, M. (2018). Damage-responsive elements in Drosophila regeneration. Genome Res. 28, 1852–1866. doi:10.1101/gr.233098.117

PubMed Abstract | CrossRef Full Text | Google Scholar

Wagner, E. F., and Nebreda, Á. R. (2009). Signal integration by JNK and p38 MAPK pathways in cancer development. Nat. Rev. Cancer 9, 537–549. doi:10.1038/nrc2694

PubMed Abstract | CrossRef Full Text | Google Scholar

Wells, B. S., and Johnston, L. A. (2012). Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53. Dev. Biol. 361, 263–276. doi:10.1016/j.ydbio.2011.10.012

PubMed Abstract | CrossRef Full Text | Google Scholar

Worley, M. I., Alexander, L. A., and Hariharan, I. K. (2018). CtBP impedes JNK- and Upd/STAT-driven cell fate misspecifications in regenerating Drosophila imaginal discs. Elife 7, e30391. doi:10.7554/eLife.30391

PubMed Abstract | CrossRef Full Text | Google Scholar

Worley, M. I., Everetts, N. J., Yasutomi, R., Chang, R. J., Saretha, S., Yosef, N., et al. (2022). Ets21C sustains a pro-regenerative transcriptional program in blastema cells of Drosophila imaginal discs. Curr. Biol. 32, 3350–3364.e6. doi:10.1016/J.CUB.2022.06.040

PubMed Abstract | CrossRef Full Text | Google Scholar

Zeitlinger, J., and Bohmann, D. (1999). Thorax closure in Drosophila: Involvement of fos and the JNK pathway. Development 126, 3947–3956. doi:10.1242/dev.126.17.3947

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, L., Chen, J., and Fu, H. (1999). Suppression of apoptosis signal-regulating kinase 1-induced cell death by 14-3-3 proteins. Proc. Natl. Acad. Sci. U. S. A. 96, 8511–8515. doi:10.1073/PNAS.96.15.8511

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, P., and Edgar, B. A. (2022). Insect gut regeneration. Cold Spring Harb. Perspect. Biol. 14, a040915. doi:10.1101/CSHPERSPECT.A040915

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, R., Luo, D., Miao, R., Bai, L., Ge, Q., Sessa, W. C., et al. (2005). Hsp90-Akt phosphorylates ASK1 and inhibits ASK1-mediated apoptosis. Oncogene 24, 3954–3963. doi:10.1038/sj.onc.1208548

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: apoptosis, regeneration, ASK1 (apoptosis signal regulating kinase 1), p38, wound healing, JNK, ROS, reactive oxygen species

Citation: Serras F (2022) The sooner, the better: ROS, kinases and nutrients at the onset of the damage response in Drosophila. Front. Cell Dev. Biol. 10:1047823. doi: 10.3389/fcell.2022.1047823

Received: 18 September 2022; Accepted: 11 October 2022;
Published: 24 October 2022.

Edited by:

Antonio Baonza, Spanish National Research Council (CSIC), Spain

Reviewed by:

Andres Dekanty, CONICET Santa Fe, Argentina
Andreas Bergmann, University of Massachusetts Medical School, United States

Copyright © 2022 Serras. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Florenci Serras, ZnNlcnJhc0B1Yi5lZHU=

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.