Zhigang Xu
Anthony W. Peng
Wei Xiong- 1Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China
- 2Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, Aurora, CO, United States
- 3School of Life Sciences, IDG/McGovern Institute for Brain Research at Tsinghua, Tsinghua University, Beijing, China
Editorial on the Research Topic
Hair Bundles—Development, Maintenance, and Function
Hair bundles are hairy-looking cell protrusions at the apical surface of mechanosensitive hair cells, consisting of hundreds of actin-based stereocilia and one microtubule-based kinocilium in each cell (Flock and Cheung, 1977). The kinocilium is important for hair bundle development as well as planar cell polarity (PCP) establishment, while the stereocilia are indispensable for mechano-electrical transduction (MET) (Hudspeth and Jacobs, 1979; Jones et al., 2008). The tightly-regulated process of hair bundle development started to be appreciated after Lew Tilney’s seminal work (Tilney et al., 1992). With the rapid progress of genetic, proteomic, and microscopic techniques, many proteins have been identified to play important roles in the development, maintenance, and/or function of hair bundles (Barr-Gillespie, 2015; McGrath et al., 2017; Velez-Ortega and Frolenkov, 2019). Nevertheless, a lot more remain unknown about this fantastic subcellular structure responsible for sound and balance detection.
In the present Research Topic, Landin Malt et al. show that non-canonical Wnt signaling regulates cochlear outgrowth and PCP through Gsk3β inhibition. Zhang et al. investigate the role of RNA-binding protein RBM24 in hair cell development, and show that Rbm24a deficiency in zebrafish affects development of hair cells as well as stereocilia through regulating the stability of its downstream target mRNAs. Li et al. show that GRXCR2 interacts with CLIC5 at the base of stereocilia and plays an essential role in stereocilia development. Kannan-Sundhari et al. reveal that bromodomain protein BRD4 is essential for stereocilia development as well as hair cell function, and Liu et al. show that lysyl oxidase LOXL3 inactivation leads to hair cell deficits including stereocilia disorganization. Wang and Zhou review the role of kinocilia in the development of hair cells and related diseases, while Cirilo et al. review the functional role of class III myosins in the stereocilia. Together, these works help us to learn more about the underlying mechanism of hair bundle development and maintenance.
The mechanic stimuli such as sound deflect hair bundles, then open the MET channels localized at the tips of shorter row stereocilia, resulting in influx of cations into the hair cells (Hudspeth and Jacobs, 1979; Beurg et al., 2009). Peng et al. show that hair bundles stimulated with a fluid jet exhibit spatial non-uniformities and two viscoelastic-like mechanisms, which are important for us to understand the mechanics of the mammalian hair bundles. TMC1/TMC2 have been suggested to be the pore-forming components of MET channels (Pan et al., 2018; Jia et al., 2020). In the zebrafish, there are one Tmc1 and two Tmc2 orthologs, and Zhu et al. show that Tmc reliance is biased by the hair cell subtype and position in the zebrafish inner ear. These works shed light on the mechanism of how stereocilia respond to mechanical stimuli and result in MET.
Our understanding of the hair bundles cannot improve without technical advances. Electron microscopy (EM) has been extensively used to understand the development, maintenance, and function of hair bundles. Ivanchenko et al. systematically summarize different EM techniques commonly used in studying hair bundles such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and focused-ion-beam scanning electron microscopy (FIB-SEM), and provide detailed protocols for sample preparation, treatment, and imaging. Miller et al. compare stereocilia measurements from living tissue, tissue mildly-fixed for fluorescent imaging, or tissue strongly-fixed for SEM, and illustrate the requirement of high accuracy to understand hair bundle mechanotransduction. Lin et al. turn to structural biology and reveal that the harmonin homology domain (HHD) of PDZD7 binds lipid and mediates the localization of PDZD7 to the plasma membrane, therefore provide mechanistic explanations for human deafness-associated mutations in PDZD7.
In the present Research Topic, researchers also show some other interesting findings. Li et al. show that albeit specially expressed in the inner ear hair cells and localized at the tips of stereocilia, annexin A4 (ANXA4) is dispensable for development and function of stereocilia and hair cells. Moreover, Gilbert et al. show that actin crosslinking family protein 7 (ACF7) is localized to the cuticular plate and the circumferential band in hair cells, but its inactivation does not have a significant effect on the hair bundle or hair cells. These studies suggest that not all hair cell-specific or hair bundle-enriched proteins are essential for hair cells to develop or function. Nevertheless, possibilities remain that these proteins are required for hair cell processes that were not examined in these studies.
In summary, this Research Topic presents some recent advances in the development, maintenance, and function of hair bundles. However, despite the rapid progress in this field, there is much more to learn. For example, how are the heights of stereocilia in different rows precisely controlled? How are the stereocilia row numbers determined? How is the MET machinery organized and regulated? We hope that we can have answers for these questions in the near future.
Author Contributions
All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Acknowledgments
We thank all the contributing authors and reviewers for their support in this Research Topic.
References
Barr-Gillespie, P.-G. (2015). Assembly of Hair Bundles, an Amazing Problem for Cell Biology. MBoC 26 (15), 2727–2732. doi:10.1091/mbc.E14-04-0940
Beurg, M., Fettiplace, R., Nam, J.-H., and Ricci, A. J. (2009). Localization of Inner Hair Cell Mechanotransducer Channels Using High-Speed Calcium Imaging. Nat. Neurosci. 12 (5), 553–558. doi:10.1038/nn.2295
Flock, A., and Cheung, H. C. (1977). Actin Filaments in Sensory Hairs of Inner Ear Receptor Cells. J. Cel Biol 75 (2 Pt 1), 339–343. doi:10.1083/jcb.75.2.339
Hudspeth, A. J., and Jacobs, R. (1979). Stereocilia Mediate Transduction in Vertebrate Hair Cells (Auditory System/cilium/vestibular System). Proc. Natl. Acad. Sci. 76 (3), 1506–1509. doi:10.1073/pnas.76.3.1506
Jia, Y., Zhao, Y., Kusakizako, T., Wang, Y., Pan, C., Zhang, Y., et al. (2020). TMC1 and TMC2 Proteins Are Pore-Forming Subunits of Mechanosensitive Ion Channels. Neuron 105 (2), 310–321. doi:10.1016/j.neuron.2019.10.017
Jones, C., Roper, V. C., Foucher, I., Qian, D., Banizs, B., Petit, C., et al. (2008). Ciliary Proteins Link Basal Body Polarization to Planar Cell Polarity Regulation. Nat. Genet. 40 (1), 69–77. doi:10.1038/ng.2007.54
McGrath, J., Roy, P., and Perrin, B. J. (2017). Stereocilia Morphogenesis and Maintenance through Regulation of Actin Stability. Semin. Cel Dev. Biol. 65, 88–95. doi:10.1016/j.semcdb.2016.08.017
Pan, B., Akyuz, N., Liu, X.-P., Asai, Y., Nist-Lund, C., Kurima, K., et al. (2018). TMC1 Forms the Pore of Mechanosensory Transduction Channels in Vertebrate Inner Ear Hair Cells. Neuron 99 (4), 736–753. doi:10.1016/j.neuron.2018.07.033
Tilney, L. G., Tilney, M. S., and DeRosier, D. J. (1992). Actin Filaments, Stereocilia, and Hair Cells: How Cells Count and Measure. Annu. Rev. Cel. Biol. 8, 257–274. doi:10.1146/annurev.cb.08.110192.001353
Keywords: hair cells, hair bundles, stereocilia, kinocilium, mechano-electrical transduction
Citation: Xu Z, Peng AW and Xiong W (2021) Editorial: Hair Bundles—Development, Maintenance, and Function. Front. Cell Dev. Biol. 9:800410. doi: 10.3389/fcell.2021.800410
Received: 23 October 2021; Accepted: 28 October 2021;
Published: 15 November 2021.
Edited and Reviewed by:
Philipp Kaldis, Lund University, SwedenCopyright © 2021 Xu, Peng and Xiong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Zhigang Xu, eHV6Z0BzZHUuZWR1LmNu