Abstract
Cerebral ischemia is one of the leading causes of death. Reperfusion is a critical stage after thrombolysis or thrombectomy, accompanied by oxidative stress, excitotoxicity, neuroinflammation, and defects in synapse structure. The process is closely related to the dephosphorylation of actin-binding proteins (e.g., cofilin-1) by specific phosphatases. Although studies of the molecular mechanisms of the actin cytoskeleton have been ongoing for decades, limited studies have directly investigated reperfusion-induced reorganization of actin-binding protein, and little is known about the gene expression of actin-binding proteins. The exact mechanism is still uncertain. The motor cortex is very important to save nerve function; therefore, we chose the penumbra to study the relationship between cerebral ischemia-reperfusion and actin-binding protein. After transient middle cerebral artery occlusion (MCAO) and reperfusion, we confirmed reperfusion and motor function deficit by cerebral blood flow and gait analysis. PCR was used to screen the high expression mRNAs in penumbra of the motor cortex. The high expression of cofilin in this region was confirmed by immunohistochemistry (IHC) and Western blot (WB). The change in cofilin-1 expression appears at the same time as gait imbalance, especially maximum variation and left front swing. It is suggested that cofilin-1 may partially affect motor cortex function. This result provides a potential mechanism for understanding cerebral ischemia-reperfusion.
Introduction
Cerebral ischemia (ischemic stroke) is one of the leading causes of death and disability worldwide (Wang et al., 2018; Virani et al., 2020). Mainly because of the expanding and aging population, the absolute number of related deaths is increasing (Feigin et al., 2014; Vivanco-Hidalgo et al., 2019). According to China stroke statistics, more than 3,010,000 inpatients with stroke were admitted to hospitals during 2018; among them, 81.9% had ischemic stroke (Wang et al., 2020). Unfortunately, therapeutic options for ischemic stroke are limited (Wang et al., 2019). In the last 5 years, reperfusion therapies, either intravenous thrombolysis or mechanical thrombectomy, have been the first line of care in a growing number of eligible acute ischemic stroke patients (Powers et al., 2018; Chamorro et al., 2020). There are potential risks in the application of reperfusion (Huang et al., 2019; Thorén et al., 2020). Reperfusion plays a biphasic role: it is beneficial in the acute stage but is potentially deleterious during recovery. Ischemia-reperfusion may result in reperfusion injury, which manifests as hemorrhagic transformation, brain edema, infarct progression, and neurologic worsening (Choi and Pile-Spellman, 2018). Most of these changes are caused by the energy exhaustion of neurons, followed by Ca2+ entry (Stegner et al., 2019), cell edema (Liebeskind et al., 2019) and excitotoxicity (Lai et al., 2014), neuroinflammation (Jayaraj et al., 2019), apoptosis (Chen et al., 2020), and autophagy (Yan et al., 2013; He et al., 2020). While irretrievable neuronal loss occurs after a series of spatiotemporal pathological changes, especially in the infarct core area where blood flow drops quickly, the surrounding hypoperfused penumbra region (peri-infarct area) is at risk of delayed cell death. Neuroprotection aims to preserve the penumbra (Chamorro et al., 2020), especially the penumbra region in the motor cortex. Neuroprotection includes not only reducing injury (such as anti-inflammation and anti-apoptotic), but also promoting restoration (such as neurogenesis and regeneration). Neurogenesis under physiological and pathological conditions was found in hippocampal and motor cortex (Chamaa et al., 2018; Lv et al., 2019; Wu et al., 2020), but different results were reported (Sorrells et al., 2018). MCAO model leads to the injury of cortex, basal ganglia (Longa et al., 1989; Ma et al., 2020), and motor deficit induced by MCAO is partially related to motor cortex. Rodent motor cortex plays an important role in motor control (Peters et al., 2017; Bundy et al., 2019; Veuthey et al., 2020). If the motor cortex injury could be treated in time, motor function may be recovery gradually (Wang et al., 2021). These injuries are related to oxidative stress (Chamorro et al., 2016), excitotoxicity (Lai et al., 2014), and neuroinflammation (Jayaraj et al., 2019) induced by reperfusion. All of these factors will cause changes in actin-binding proteins.
Actin-binding proteins (such as cofilin-1) play an important role in the regulation of skeletal proteins in neurons (Bernstein and Bamburg, 2010; Shirao et al., 2017), in the injury and repair of neurons (Bamburg et al., 2010; Alhadidi et al., 2016; Shah and Rossie, 2018), apoptosis and autophagy (Head et al., 2009; Sanchez-Varo et al., 2012; Madineni et al., 2016; Wang et al., 2016), and in changes in mitochondrial function and structure (Hoffmann et al., 2019). Actin-binding proteins also play an important role in the regeneration and development of dendritic spines in a pathological state (Sekino et al., 2007; Koganezawa et al., 2017; Kreis et al., 2019). Existing studies have shown that these actin-binding proteins are changed in degenerative neuropathy (Shaw and Bamburg, 2017; Schaffer et al., 2018; Inoue et al., 2019). Cofilin-1 is abnormal after ischemia and affects the morphological integrity, receptor transport and signal transduction of spines in synapses (Shaw and Bamburg, 2017; Won et al., 2018; Shu et al., 2019). Arp2/3 is also commonly involved in actin cycling/turnover (Korobova and Svitkina, 2008; Spence et al., 2016; Konietzny et al., 2017). Additionally, it was found that drebrin plays an important role in developing cerebral cortex and neurodegenerative diseases (Chimura et al., 2015; Sinclair et al., 2015; Inoue et al., 2019). The abnormal changes in actin-binding proteins, which are major regulators of actin dynamics, may result in dendritic injury of neurons during ischemic stroke (Shu et al., 2019). The changes in nerve function induced by actin-binding proteins are not the same in different brain regions during cerebral ischemia-reperfusion. Limited studies have focused on actin-binding proteins in the motor cortex, and little is known about the gene expression of these proteins.
At present, it is not clear whether there are changes in these actin-binding proteins during cerebral ischemia-reperfusion in the motor cortex penumbra. In this study, we aimed to identify the regulatory factors (cofilin-1, Arp2/3, and drebrin-like) of actin cycling/turnover related to cerebral ischemia-reperfusion. And the changes in these proteins and mRNAs in the motor cortex penumbra need to be observed. So that we could investigate the pathological processes and understand the effects of actin-binding proteins during cerebral ischemia-reperfusion.
Materials and Methods
Grouping and Experimental Process
Experiments were performed in adult male Sprague–Dawley rats (257 ± 19 g, Shanghai SLAC Laboratory Animal Co., Ltd.). Animals were housed in institutional animal facilities on a 12 h day/night cycle with ad libitum access to food and water. All experimental procedures were performed in accordance with animal welfare and ethical principles and were approved by the Animal Use and Management Committee of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine.
All rats were randomly divided into two groups: the control group (control) and the middle cerebral artery occlusion (MCAO) group. Control group (n = 8): no MCAO. MCAO group (n = 8): MCAO and reperfusion.
The brief procedure is as follows (also shown in Figure 1A): At the T0 time point, cerebral blood flow (CBF) measurements were prepared (after anesthesia, a small piece of scalp was removed, and detection points were exposed), and the rats then recovered for 3 days, and gait training was conducted for 7 days until the T1 time point with CatWalkTM system XT (Noldus, Wageningen, Netherlands). After that, all rats were analyzed for gait and rested for 7 days. At the T2 time point, gait analysis and neurological deficit scores were measured. CBF was measured after anesthesia in both groups, and surgery was performed in the MCAO group. At the T3 time point, MCAO was performed to block blood flow in the MCAO groups, and the CBF of both groups was measured. At the T4 time point (1.5 h after MCAO), the nylon filament suture was removed, cerebral blood flow was reperfused, CBF was measured again, and the neurological deficit score was measured after the rats regained consciousness. At the T5 time point (3 h after reperfusion), the neurological deficit score was measured. At the T6 time point (24 h after reperfusion), gait and scores were measured.
FIGURE 1
Middle Cerebral Artery Occlusion and Reperfusion
The rat model of middle cerebral artery (MCA) ischemia-reperfusion was established according to the literature (Longa et al., 1989; Noh et al., 2020). Rats underwent anesthesia by 4% isoflurane (RWD Life Science, 217180501) with anesthesia machine (R500 RWD Life Science Co., LTD., Shenzhen, China). The right common carotid artery (CCA) and internal carotid artery (ICA) were exposed via a midline incision in the neck. In the right external carotid artery (ECA), a suture was used to tie an encased knot at the distal end and a slipknot at the proximal end. The right CCA and ICA were temporarily clamped by a microvascular clip and cut approximately 5 mm from the bifurcation of the right ECA. A nylon filament suture (poly-L-lysine-coated nylon monofilament 0.36 mm, Beijing Cinontech Co., Ltd., Beijing, China) was inserted into the incision of the right ECA. The nylon filament suture was advanced from the right ECA through the CCA and up to the ICA for a distance of 18 ± 0.5 mm to block the origin of the MCA. The right MCA was occluded for 90 min. After that, cerebral blood flow was restored by withdrawal of the nylon thread. The temperature of the animals was maintained at 37 ± 0.5°C through a feedback-adjusted heating blanket (Xu et al., 2013).
Detection of Cerebral Blood Flow and Pathological Confirmation After Operation
Cerebral Blood Flow Measurements
CBF was measured in the right hemisphere of the experimental rats. A moor VMS-LDFTM laser Doppler blood flow monitor (Moor Instruments, Devon, United Kingdom) was used to detect the CBF of rats under inhalation anesthesia. Anesthesia was achieved by face mask-delivered isoflurane (2% induction and 1.5% maintenance in 80% N2 and 20% O2). After placing the animal in the stereotaxic frame, the dorsal surface of the skull was exposed through a midline cut. A laser Doppler flowmetry (LDF) probe was situated on the skull, 3 mm posterior to the bregma and 5 mm lateral to the midline (Nishimura et al., 2000). The reperfusion was confirmed by CBF measurement after pulling out the suture from ECA.
TTC Staining
We determined the infarct volume after 24 h of reperfusion with the 2,3,5-triphenyltetrazolium chloride (TTC) method (Longa et al., 1989). Following neurological function evaluation, four rats in each group were deeply anesthetized by an intraperitoneal dose of 400 mg/kg chloral hydrate and then sacrificed. Each brain was removed and sliced into 2 mm sections using a rodent brain matrix slicer (RBM-4000C; ASI Instruments, Warren, MI, United States) (Xu et al., 2013).
Brain tissue sections were immersed in 10 ml of 2% TTC (Beijing Solarbio Life Science and Technology Co., Ltd., Beijing, China) phosphate buffer solution (pH 8.7) and incubated at 37°C in the dark for approximately 30 min. The brain tissue was turned upside down every 10 min. At this time, the normal brain tissue was dyed dark red, and the cerebral infarction area was not stained (gray white). Then, the brain tissue was immersed in 4% paraformaldehyde phosphate buffer (pH 7.4) and fixed at 4°C for 24 h.
A coronal section through optic chiasm was selected. Areas of red and white staining were measured using an image processing program (ImageJ 1.52P, NIH, United States). The percent of infarction is given by the equation: infarct area (%) = infarct area/total area of slice × 100 (Xu et al., 2013).
The coronal sections through optic chiasm were used for cortex penumbra samples (Nash et al., 2018; Sanchez-Bezanilla et al., 2019). The area was shown in Figure 1B. These samples were used for PCR, immunohistochemistry (IHC), and Western blot (WB).
Assessment of Behavioral Change and Assessment of Gait Change
Neurological Deficit Score
The method that we used in this study for behavioral assessment of focal cerebral ischemia in rats has been described previously. All rats were evaluated by the same trained person. The neurological examination results were scored according to the five point system: zero point, no obvious defect; one point, forelimb flexion; two points, forelimb flexion and lateral push resistance reduction; three points, forelimb flexion, lateral push resistance decreased, unilateral rotation occurred in three consecutive tests; four points, three points symptoms plus decreased consciousness (Yang et al., 2000).
Gait Training and Gait Analysis
Before the first recording of the gait, rats were habituated to the laboratory and walkway conditions and trained. Rats were trained for 7 days to cross the runway of the CatWalkTM system, a video-based analysis system, to assess static and dynamic gait parameters. Briefly, the equipment was located in a dark and silent room. Rats were made to travel through a 1.5 m long enclosed glass plate. Animal movement was captured with a camera placed under the walkway and connected to the data acquisition station. A burrow-like house was placed with food at the end of the CatWalkTM runway, which the animals recognized as a safe shelter.
On the last day of training, crossing the CatWalkTM system (three runs per animal) was recorded; these measurements were used as baseline values. Three crossings of the CatWalkTM runway without interruption/hesitation were required for a valid kinematic gait analysis in each animal. Functional studies were performed three times in both groups of rats. A series of behavioral tests was performed before MCAO (T2) and 24 h after reperfusion (T6) (Domin et al., 2016). Gait parameters were labeled right forepaw (RF), right hind paw (RH), left forepaw (LF), and left hind paw (LH). Data were analyzed using CatWalkTM XT 10 software (Fluri et al., 2017).
Gene and Protein Expression of Actin-Binding Proteins in the Motor Cortex
Real-Time PCR and Measurement of mRNA
Real-time PCR was carried out using a real-time PCR system (Light Cycler 480 Instrument II, Roche). The real-time cycler was programmed according to the kit protocol. The threshold cycle number was determined using SDS v1.4 Software, and the reactions were performed in triplicate. Total RNA (5 μg) was reverse transcribed into cDNA by using the RevertAid First Strand cDNA synthesis kit (Invitrogen K1622). For RT-PCR of the cDNA, primer pairs were designed to generate intron-spanning products of 101–150 bp (primer sequences are listed in Table 1). The generation of specific PCR products was confirmed by the melting curve. The expression ratio was calculated according to the formula 2(Rt–Et)/2(Rn–En) as described previously (Livak and Schmittgen, 2001). Transcripts with a twofold increase in expression were considered to be upregulated, and those with a 0.5-fold decrease in expression were considered to be downregulated (Yin et al., 2009).
TABLE 1
| Gene | Primer sequence (5′→3′) forward/reverse |
| COF1 | GACTGCCGCTATGCTCTCTA/CTTGATGGCATCCTTGGAGC |
| ARPC1A | CGGCTCATCTCTGTCTGCTA/AAAACGTTGTTGGGATGCCA |
| ARPC2 | GTGAACAACCGCATCATCGA/AGGACGCCATCAAAATCTGC |
| ARPC3 | GCGGACAGGACCTTGATCTA/TGGAGTTGCACTTTTGGAGC |
| ARPC4 | ACTTCTCTTCCCAGGTCGTG/ACCCGGACAGAATTGATGGA |
| ARPC5L | TCACTGGACAGGAATGGCAT/AAGCCTTTTCATGCCACTGG |
| DBNL | TACCAGAAGACCAATGCCGT/TCTCCTCCTCCTTCTCAGCT |
| ENAH | ATTCAGAGTGGTGGGCAGAA/TTGCTGCCAAAGTTGAGACC |
| WASL | GGTGACCATCAAGTTCCAGC/GGCCATCAGACACGGATTTC |
| BRK1 | GCGAGAGATTCACCAGGACT/TCTCACCCTTTGTCACCCTC |
| GAPDH | TGCCACTCAGAAGACTGTGG/TTCAGCTCTGGGATGACCTT |
Primers for real-time PCR (RT-PCR).
COF1, cofilin-1; ARPC1A, actin-related protein 2/3 complex, subunit 1A; ARPC2, actin-related protein 2/3 complex, subunit 2; ARPC3, actin-related protein 2/3 complex, subunit 3; ARPC4, actin-related protein 2/3 complex, subunit 4; ARPC5L, actin-related protein 2/3 complex, subunit 5L; DBNL, developmentally regulated brain like protein; ENAH, enabled homolog; WASL, neural Wiskott–Aldrich syndrome protein; BRK1, Brick1 subunit of SCAR/WAVE actin nucleating complex (Brick1 is also known as hematopoietic stem cell protein 300, HSPC300); GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Immunohistochemistry and Measurement of Proteins
The target area was embedded in paraffin for standby. The tissue was continuously sliced at a thickness of 3 μM. The tissue was pasted on the slide and baked at 60°C for 1 h to make the tissue adhere tightly to the slide. The slices were dehydrated with 100, 90, 80, and 70% gradient alcohol for 30 min and then washed with tap water for 30 min. The slices were placed into a container containing citric acid hydrochloric acid buffer solution, placed in a high-pressure cooker at 100°C for 6 min, cooled to room temperature, and then removed. H2O2 solution (3%) was added dropwise, incubated in the dark for 5 min, washed with distilled water for 5 min, and washed with buffer solution three times for 5 min each time. After being sealed with 5% normal goat serum and placed at room temperature for 30 min, the excess liquid was removed. The primary antibody (anti-cofilin-1 primary antibody, ab42824, Abcam Inc., 1:100; anti drebrin primary antibody, ab11068, Abcam Inc., 1:50; Anti-ARPC2 antibody, HPA008352, Sigma Inc., 1:400) was added dropwise, placed overnight in a 4°C incubator, and placed in a 37°C incubator for 30 min. The buffer solution was washed three times for 5 min each time. Fifty microliters of biotin-labeled secondary antibody was added dropwise and incubated at 37°C for 30 min, and the buffer solution was washed three times for 5 min each. Horseradish peroxidase labeled streptase ovalbumin working solution was added and incubated at 37°C for 30 min, and the buffer solution was washed three times for 5 min each. One drop each of reagents A, B, and C was added to the kit in turn to avoid light, mixed well and added to the slices. The color reaction was terminated after 5 min at room temperature. Hematoxylin staining was performed for 3 min. The slices were dehydrated by 70, 80, 90, and 100% gradient alcohol successively, xylene was added to clear the slides for 5–8 min, and then the slices were dried naturally. Neutral gum was added to the slides, and they were covered with a clean coverslip (Han et al., 2009).
Western Blot and Measurement of Cofilin
Western blot analysis was used to investigate the cofilin protein expression in rat brain. After being separated by SDS-PAGE, the proteins were electrotransferred onto a PVDF membrane. Then, the membrane was blocked with 5% bovine serum albumin (BSA) for 1 h and analyzed using specific primary antibodies, including anti-cofilin (ab42824, Abcam) and anti-β-actin (4,967, Cell Signaling Technology). Horseradish peroxidase (HRP)-conjugated secondary antibodies were applied, and the membrane was visualized using an electrogenerated chemiluminescence (ECL) detection system.
Statistical Analysis
The data are presented as the mean ± SD. One-way ANOVA (analysis of variance) followed by the least significant difference (LSD) test or the Games-Howell test (depending on the data and on the hypothesis tested). Post hoc analysis was used to analyze the significance of the neurological deficit score, CBF and gait measurement among the two groups. P < 0.05 was considered indicative of significance.
Results
According to the schematic (Figure 1A), modeling, CBF detection, behavioral evaluation, infarct area analysis, and molecular biology of actin-binding proteins in the penumbra were performed in rats at specific time points (T0–T6).
CBF of the Motor Cortex Decreased, Obvious Infarction Appeared
At T2 before the operation, there was no significant difference in the cerebral blood flow of rats between the groups. At T3, MCAO significantly reduced cerebral blood flow compared to that before ischemia compared with the control group. At T4 (1.5 h after MCAO and the beginning of reperfusion), cerebral blood flow in the MCAO group was still significantly lower than that in the control group. After reperfusion, the cerebral blood flow in the MCAO group recovered significantly, but it was still lower than that before the operation (Figure 1C). TTC staining may reflect the infarct region in the rat brain (Figure 1E). There were significant differences in the infarct area (%) between rats in the control and model groups (P < 0.05, Figure 1D).
Obvious Behavioral Changes During Cerebral Ischemia-Reperfusion
To evaluate the change in behavior comprehensively, we used a relatively rough neurologic defect score and relatively reliable gait analysis. Rat gait prints were collected by using the CatWalkTM System. The process of limbs contacting the runway in a temporal series is shown in Figure 2A (upper figure), and the process of the spatial series is shown in Figure 2A (lower figure).
FIGURE 2
Neurological Deficit Score Significantly Increased
The score was determined in conscious rats. At T2, the scores of all rats in the control and MCAO groups were 0. At T4 (beginning reperfusion), the scores of the MCAO group were significantly increased (P < 0.05). At T5 (3 h after reperfusion), the score of MCAO rats recovered. At T6 (24 h after reperfusion), the score of MCAO rats was still increased compared with that of control rats, and there was a significant difference (P < 0.05) (Figure 2B).
Average Speed Decreased, Variation in Average Speed Increased
There was no significant difference in average speed between T1 and T2 and no significant difference in rats between the control group and MCAO group at T1 and T2 (Figure 2C). At T6 (24 h after reperfusion), MCAO decreased the average speed significantly compared with the control group and compared with itself at T1 and T2 (Figure 2C).
The maximum variation is the maximum variation in the average speed of the selected steps in the trial. It can reflect the speed of speed change. After training, control rats are usually able to cross the runway at an approach uniform velocity. Maximum variation is generally 10–20% (Figure 2D). There was no significant difference in maximum variation between T1 and T2. There was no significant difference between groups. At T6 (24 h after reperfusion), the maximum variation increased to some extent, and MCAO led to walking speed that was sometimes fast and sometimes slow. MCAO increased the maximum variation significantly compared with T2. There was a significant difference in maximum variation compared with the control group at T6 (Figure 2D).
Number of Steps Increased, Limb Swing Expanded
The number of steps is the total number of selected steps in the run. Limb swing is the duration in seconds of no contact of the paw with the glass plate. After cerebral ischemia-reperfusion, the balance of rats was poor, walking speed was decreased, number of steps increased, and limb swing expanded. There was no significant difference in the number of steps between T1 and T2, and there was no significant difference in the number of steps between the control group and MCAO group at T1 and T2 (Figure 2E). At T6 (24 h after reperfusion), MCAO increased the number of steps significantly compared with the control group and compared with T1 and T2 (P < 0.05) (Figure 2E).
The left forelimb was the affected side of the MCAO model. There was no significant difference in LF swing between T1 and T2. There was no significant difference between the groups (Figure 2F). At T6 (24 h after reperfusion), MCAO significantly increased LF swing compared with the control group and compared with T1 and T2 (Figure 2F). The results for RF swing (Figure 2F), LH swing (Figure 2F), and RH swing (Figure 2F) were the same as those for RF swing, and MCAO significantly increased swing compared with the control group.
Overexpression of COF1 mRNA and COF1 in the Motor Cortex After Cerebral Ischemia-Reperfusion
Compared with the control group, the mRNA levels of COF1 were significantly increased by MCAO and reperfusion (Figure 3A). In addition, the mRNA expression levels of ARPC2, ARPC3, ARPC4, ARPC5L, and DBNL were upregulated less than twofold compared with the control group. The mRNA expression levels of ENAH, WASL, and BRK1 were downregulated less than 0.5-fold compared with the control group.
FIGURE 3
To further verify the mRNA results, three mRNAs (COF1, ARPC2, and DBNL) demonstrating closest to twofold change were selected to verify the protein expression in IHC. For cofilin-1, which is closely related to the polymerization and depolymerization of actin and has obvious changes in this study (Figure 3A), we conducted a semiquantitative analysis of the protein expression using IHC (Figures 3B,E).
The results showed that the positive expression area of COF1 increased significantly after cerebral ischemia-reperfusion (P < 0.05) (Figures 3B,E). The positive expression areas of ARPC2 and DBNL did not significantly increase after cerebral ischemia-reperfusion (Figures 3B,E).
To further verify the IHC results, COF1 were selected to verify the protein expression in WB. The results showed that the optical density ratio (normalized by β-actin) of COF1 increased significantly after cerebral ischemia-reperfusion (P < 0.05) (Figures 3C,D).
Discussion
Research on chemical drugs for cerebral ischemia has been conducted for decades. However, currently, the most effective drugs are anticoagulants and thrombolytics, which require a tightly controlled time window (Kepplinger et al., 2016). If used beyond the time window, these drugs will increase the risk of intracranial hemorrhage (Choi and Pile-Spellman, 2018). Vascular recanalization often results in reperfusion injury. Although other drugs (anti-injury or promoting neuron regeneration) have achieved good results in animal experiments, they have not been clinically verified (Kaur et al., 2013; Zhou et al., 2018). These biological processes are closely related to actin-binding proteins (e.g., cofilin-1) through dephosphorylation by specific phosphatases (Alhadidi et al., 2016). Although studies of the molecular mechanisms of the actin cytoskeleton have been ongoing for decades, limited literature has directly investigated cerebral ischemia-reperfusion-induced reorganization of actin-binding proteins in the cortex penumbra, and little is known about the gene expression of actin-binding proteins. Our research explored whether there are changes in actin-binding proteins induced by cerebral ischemic reperfusion.
Some studies have shown that cerebral blood flow decreased nearly 80% after MCAO, and obvious infarct size was found with TTC staining (Beard et al., 2020). Using spontaneously hypertensive rats (Cipolla et al., 2018) and aged hypertensive rats (Chan et al., 2018), similar results were found. Cerebral blood flow and TTC staining results were stronger predictors of brain damage in MCAO rats. Our results are consistent with previous publications.
Sports injuries caused by cerebral ischemia are still the main cause of disability. Upper motor impairment is the most common source of disability after cerebral ischemia (Langhorne et al., 2009; Lin et al., 2019). Similar to human, cerebral ischemia also changed gait in rats. At present, there are limited studies on MCAO using gait analysis. In the past, most studies have focused on static parameters of gait (print area, maximal contact area, and stride length) (Domin et al., 2016; Fan et al., 2018; Hu et al., 2019). Few studies have focused on dynamic parameters (Gao et al., 2020). Our research highlights whole-body dynamic parameters (average speed, maximum variation, number of steps, swing) in gait analysis.
With the help of gait analysis, we can quickly and sensitively evaluate sports injuries and supplement the neurological deficit score. After MCAO on the right brain of the rat, the left limb (especially the left forelimb) has dyskinesia. The left forelimb flexion and extension movement is unfavorable. The movement continuity is poor, and the movement speed is sometimes fast and sometimes slow. Our results partly verified previous study (Parkkinen et al., 2013).
The sports injury (i.e., gait changes) caused by cerebral ischemia is closely related to necrosis of the ischemic area, and the necrotic area will not be repaired (Boned et al., 2017; Baron, 2018). However, the ischemic penumbra belongs to the boundary area. Penumbra may recover after proper treatment or may be aggravated and necrotic (Lo, 2008; Candelario-Jalil and Paul, 2020). Therefore, it is of great value to study the penumbra.
The cytoskeleton has attracted our attention in studying penumbra. It has unique function in forming the major neuron structure. It is essential for protein localization and the transport of molecules between dendrites and axons (Madineni et al., 2016). A large number of studies have confirmed that neural structural reorganization is the basis of functional plasticity (Yamada and Kuba, 2016; Gipson and Olive, 2017). Structural reorganization (e.g., spine) is closely related to skeletal proteins (especially actins). Crosstalk between actin and actin-binding proteins during neural pathology has recently attracted much attention (Carlisle and Kennedy, 2005; Alvarez and Sabatini, 2007; Mar et al., 2014; Tonnesen and Nagerl, 2016; Blanquie and Bradke, 2018). In addition, cerebral ischemia-reperfusion can lead to neural injury. Changes in actin-binding proteins would be shown (Lai et al., 2014; Chamorro et al., 2016; Jayaraj et al., 2019). The function of specific brain regions (such as the motor cortex) would be affected. The study of actin-binding proteins in the penumbra of the motor cortex is helpful to reveal the pathological processes of cerebral ischemia-reperfusion.
Recent studies have emphasized that cofilin-1 is involved in the harmful neuronal processes of ischemic stroke, especially in the penumbra (Won et al., 2018; Shu et al., 2019). Interestingly, cofilin-actin rods were not visible after 1 h, but were widely formed within 4–24 h after reperfusion (Hoffmann et al., 2019).
The cofilin-actin rods form macromolecular aggregates, and affected neurites interrupt the cytoskeleton. The rods also affect organelle transport and lead to loss of dendritic spines (Bamburg et al., 2010). Simple overexpression of cofilin-1 is sufficient to induce actin rod formation and associated neurite degeneration (Minamide et al., 2000). Ischemia also leads to loss of dendrites and spines in neurons, even with survival of the parent cell body (Tanay et al., 2006). Cofilin-actin rod suppression during the acute phase of ischemic stroke might provide neuroprotection with histological results (Kurisu et al., 2019). It has also been reported that cofilin-1 increases in oxygen glucose deprivation model of ischemia with in vitro fetal mouse neurons (Madineni et al., 2016). Our study confirmed that MCAO leads to an increase in COF1 mRNA and COF1 protein in the penumbra of the motor cortex with adult rats. These changes may be partially associated with gait imbalance and may be related to structural reorganization and functional impairment of neurons.
Cofilin-1 is an actin-binding protein normally involved in the dynamic turnover of actin filaments (actin treadmilling) and other cell functions (new functions). Phosphoregulation of cofilin is a downstream target of many transmembrane signaling processes, and it has been linked in rodent models to many different neurodegenerative and neurological disorders (Alhadidi et al., 2017; Shaw and Bamburg, 2017). Phosphorylation by LIM kinase (LIMK) causes the deactivation of cofilin, while dephosphorylation by slingshot protein phosphatase-1L (SSH-1L) or chronophin (CIN). Cofilin with active form promotes the turnover of actin filaments (Shu et al., 2019). Cerebral ischemia-reperfusion induces decline in ATP, shifts the balance of kinase/phosphatase activity toward dephosphocofilin, increases active cofilin, increases ADP-actin, and promotes cofilin oxidation and actin rod formation (Bamburg and Bernstein, 2016). Cerebral ischemia-reperfusion activates SSH-1L and CIN, causing active cofilin-1 to emerge and affecting the functions of cofilin-1 (Kanellos and Frame, 2016).
The regulatory mechanism for cofilin mRNA translation during cerebral ischemia-reperfusion is still not well studied. We first reported that the increase in cofilin-1 was associated with cerebral ischemia-reperfusion in the motor cortex. It is suggested that the increase in cofilin-1 is due to dephosphorylation of p-cofilin-1 and a large number of transcriptional mRNAs. In the nucleus, cofilin-1 is required for RNA polymerase II transcription elongation (Obrdlik and Percipalle, 2011), and the presence of cofilin-1 in the nucleus is consistent with enhanced/faster RNA polymerase II-dependent transcription (Domingues et al., 2020). After cerebral ischemia-reperfusion, a large amount of cofilin-1 accumulates in the cytoplasm, which may enter the nucleus and accelerate the transcription of mRNA (including cofilin-1 mRNA). This may be a potential mechanism for the continuous increase in cofilin-1 and damage to the cytoskeleton.
Although previous studies found that ARP2/3 and DBNL exhibit significant changes after cerebral ischemia, we determined that the mRNA and protein levels of ARP2/3 and DBNL exhibit changes, but they are not significant. It has been suggested that ARP2/3 and DBNL may not be direct or key influencing factors in MCAO- and reperfusion-induced sports injury.
Due to the limitation of such tMCAO in this research, the affected areas include cortex and basic ganglia. Whether the above results are applicable to cortex stroke remains to be further verified.
Conclusion
Cerebral ischemia-reperfusion leads to the motor cortex injury with the cofilin-1 increase in the penumbra and is partially related to dyskinesia, suggesting that cofilin-1 plays an important role during cerebral ischemia-reperfusion.
Statements
Data availability statement
The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author/s.
Ethics statement
The animal study was reviewed and approved by the Animal Use and Management Committee of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine.
Author contributions
M-SX and L-MY were responsible for the RT-PCR and Western blot validation and drafting of the manuscript. A-FC, Y-JZ, DZ, and M-MT duplicated the rat middle cerebral artery occlusion model and neurological deficit score. Y-YD contributed to the immunohistochemistry analyses. A-FC, Y-JZ, and M-MT performed the gait training and gait analysis. A-FC and M-MT completed the laser Doppler blood flow and TTC staining. M-SX, L-MY, L-BG, and C-LS contributed to the design of the study and draft of the manuscript. All authors read and approved the final manuscript.
Funding
This study was supported by the Shanghai Committee of Science and Technology Research Plan (No. 17ZR1427500), Shanghai Municipal Healthy Commission Research Plan (No. 201640128), Shanghai Talent Development Fund (No. 2017094), National Key Research and Development Program of China (No. 2018YFC2001600), and Program of Shanghai Academic Research Leader (No. 19XD1403600).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
AlhadidiQ.Bin SayeedM. S.ShahZ. A. (2016). Cofilin as a promising therapeutic target for ischemic and hemorrhagic stroke.Transl. Stroke Res.733–41. 10.1007/s12975-015-0438-2
2
AlhadidiQ.Bin SayeedM. S.ShahZ. A. (2017). The interplay between cofilin and phospho-cofilin: its role in maintaining blood brain barrier integrity.CNS Neurol. Disord. Drug Targets16279–290. 10.2174/1871527316666170117115040
3
AlvarezV. A.SabatiniB. L. (2007). Anatomical and physiological plasticity of dendritic spines.Annu. Rev. Neurosci.3079–97. 10.1146/annurev.neuro.30.051606.094222
4
BamburgJ. R.BernsteinB. W. (2016). Actin dynamics and cofilin-actin rods in alzheimer disease.Cytoskeleton73477–497. 10.1002/cm.21282
5
BamburgJ. R.BernsteinB. W.DavisR. C.FlynnK. C.GoldsburyC.JensenJ. R.et al (2010). ADF/Cofilin-actin rods in neurodegenerative diseases.Curr. Alzheimer Res.7241–250. 10.2174/156720510791050902
6
BaronJ. C. (2018). Protecting the ischaemic penumbra as an adjunct to thrombectomy for acute stroke.Nat. Rev. Neurol.14325–337. 10.1038/s41582-018-0002-2
7
BeardD. J.LiZ.SchneiderA. M.CouchY.CipollaM. J.BuchanA. M. (2020). Rapamycin induces an eNOS (Endothelial Nitric Oxide Synthase) dependent increase in brain collateral perfusion in wistar and spontaneously hypertensive rats.Stroke512834–2843. 10.1161/strokeaha.120.029781
8
BernsteinB. W.BamburgJ. R. (2010). ADF/cofilin: a functional node in cell biology.Trends Cell Biol.20187–195. 10.1016/j.tcb.2010.01.001
9
BlanquieO.BradkeF. (2018). Cytoskeleton dynamics in axon regeneration.Curr. Opin. Neurobiol.5160–69. 10.1016/j.conb.2018.02.024
10
BonedS.PadroniM.RubieraM.TomaselloA.CoscojuelaP.RomeroN.et al (2017). Admission CT perfusion may overestimate initial infarct core: the ghost infarct core concept.J. Neurointerv. Surg.966–69. 10.1136/neurintsurg-2016-012494
11
BundyD. T.GuggenmosD. J.MurphyM. D.NudoR. J. (2019). Chronic stability of single-channel neurophysiological correlates of gross and fine reaching movements in the rat.PLoS One14:e0219034. 10.1371/journal.pone.0219034
12
Candelario-JalilE.PaulS. (2020). Impact of aging and comorbidities on ischemic stroke outcomes in preclinical animal models: a translational perspective.Exp. Neurol.335113494. 10.1016/j.expneurol.2020.113494
13
CarlisleH. J.KennedyM. B. (2005). Spine architecture and synaptic plasticity.Trends Neurosci.28182–187. 10.1016/j.tins.2005.01.008
14
ChamaaF.BahmadH. F.MakkawiA. K.ChalhoubR. M.Al-ChaerE. D.BikhaziG. B.et al (2018). Nitrous oxide induces prominent cell proliferation in adult rat hippocampal dentate gyrus.Front. Cell Neurosci.12:135. 10.3389/fncel.2018.00135
15
ChamorroÁDirnaglU.UrraX.PlanasA. M. (2016). Neuroprotection in acute stroke: targeting excitotoxicity, oxidative and nitrosative stress, and inflammation.Lancet Neurol.15869–881. 10.1016/s1474-4422(16)00114-9
16
ChamorroÁLoE. H.RenúA.van LeydenK.LydenP. D. (2020). The future of neuroprotection in stroke.J. Neurol. Neurosur. Psychiatry92129–135. 10.1136/jnnp-2020-324283
17
ChanS. L.BishopN.LiZ.CipollaM. J. (2018). Inhibition of PAI (Plasminogen Activator Inhibitor)-1 improves brain collateral perfusion and injury after acute ischemic stroke in aged hypertensive rats.Stroke491969–1976. 10.1161/strokeaha.118.022056
18
ChenB.LinW.QiW.LiS.HongZ.ZhaoH. (2020). Cofilin inhibition by limk1 reduces rod formation and cell apoptosis after ischemic stroke.Neuroscience44464–75. 10.1016/j.neuroscience.2020.07.019
19
ChimuraT.LauneyT.YoshidaN. (2015). Calpain-mediated degradation of drebrin by excitotoxicity in vitro and in vivo.PLoS One10:e0125119. 10.1371/journal.pone.0125119
20
ChoiJ. H.Pile-SpellmanJ. (2018). Reperfusion changes after stroke and practical approaches for neuroprotection.Neuroimag. Clin. N. Am.28663–682. 10.1016/j.nic.2018.06.008
21
CipollaM. J.LinfanteI.AbuchowskiA.JubinR.ChanS. L. (2018). Pharmacologically increasing collateral perfusion during acute stroke using a carboxyhemoglobin gas transfer agent (SanguinateTM) in spontaneously hypertensive rats.J. Cereb. Blood Flow Metab.38755–766. 10.1177/0271678x17705567
22
DominH.PrzykazaŁJantasD.KozniewskaE.BoguszewskiP. M.ŚmiałowskaM. (2016). Neuroprotective potential of the group III mGlu receptor agonist ACPT-I in animal models of ischemic stroke: in vitro and in vivo studies.Neuropharmacology102276–294. 10.1016/j.neuropharm.2015.11.025
23
DominguesC.GeraldoA. M.AnjoS. I.MatosA.AlmeidaC.CarameloI.et al (2020). Cofilin-1 is a mechanosensitive regulator of transcription.Front. Cell Dev. Biol.8:678. 10.3389/fcell.2020.00678
24
FanQ. Y.LiuJ. J.ZhangG. L.WuH. Q.ZhangR.ZhanS. Q.et al (2018). Inhibition of SNK-SPAR signaling pathway promotes the restoration of motor function in a rat model of ischemic stroke.J. Cell. Biochem.1191093–1110. 10.1002/jcb.26278
25
FeiginV. L.ForouzanfarM. H.KrishnamurthiR.MensahG. A.ConnorM.BennettD. A.et al (2014). Global and regional burden of stroke during 1990-2010: findings from the Global burden of disease study 2010.Lancet383245–254. 10.1016/s0140-6736(13)61953-4
26
FluriF.MalzahnU.HomolaG. A.SchuhmannM. K.KleinschnitzC.VolkmannJ. (2017). Stimulation of the mesencephalic locomotor region for gait recovery after stroke.Ann. Neurol.82828–840. 10.1002/ana.25086
27
GaoB.ZhouS.SunC.ChengD.ZhangY.LiX.et al (2020). Brain endothelial cell-derived exosomes induce neuroplasticity in rats with ischemia/reperfusion injury.ACS Chem. Neurosci.112201–2213. 10.1021/acschemneuro.0c00089
28
GipsonC. D.OliveM. F. (2017). Structural and functional plasticity of dendritic spines – root or result of behavior?Genes Brain Behav.16101–117. 10.1111/gbb.12324
29
HanM.DongL. H.ZhengB.ShiJ. H.WenJ. K.ChengY. (2009). Smooth muscle 22 alpha maintains the differentiated phenotype of vascular smooth muscle cells by inducing filamentous actin bundling.Life Sci.84394–401. 10.1016/j.lfs.2008.11.017
30
HeT.LiW.SongY.LiZ.TangY.ZhangZ.et al (2020). Sestrin2 regulates microglia polarization through mTOR-mediated autophagic flux to attenuate inflammation during experimental brain ischemia.J. Neuroinflamm.17:329. 10.1186/s12974-020-01987-y
31
HeadB. P.PatelH. H.NiesmanI. R.DrummondJ. C.RothD. M.PatelP. M. (2009). Inhibition of p75 neurotrophin receptor attenuates isoflurane-mediated neuronal apoptosis in the neonatal central nervous system.Anesthesiology110813–825. 10.1097/ALN.0b013e31819b602b
32
HoffmannL.RustM. B.CulmseeC. (2019). Actin(g) on mitochondria - a role for cofilin1 in neuronal cell death pathways.Biol. Chem.4001089–1097. 10.1515/hsz-2019-0120
33
HuJ.LiC.HuaY.ZhangB.GaoB. Y.LiuP. L.et al (2019). Constrained-induced movement therapy promotes motor function recovery by enhancing the remodeling of ipsilesional corticospinal tract in rats after stroke.Brain Res.170827–35. 10.1016/j.brainres.2018.11.011
34
HuangX.YangQ.ShiX.XuX.GeL.DingX.et al (2019). Predictors of malignant brain edema after mechanical thrombectomy for acute ischemic stroke.J. Neurointerv. Surg.11994–998. 10.1136/neurintsurg-2018-014650
35
InoueS.HayashiK.FujitaK.TagawaK.OkazawaH.KuboK.-I.et al (2019). Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex.J. Neurosci.39678–691. 10.1523/JNEUROSCI.1634-18.2018
36
JayarajR. L.AzimullahS.BeiramR.JalalF. Y.RosenbergG. A. (2019). Neuroinflammation: friend and foe for ischemic stroke.J. Neuroinflammation16:142. 10.1186/s12974-019-1516-2
37
KanellosG.FrameM. C. (2016). Cellular functions of the ADF/cofilin family at a glance.J. Cell Sci.1293211–3218. 10.1242/jcs.187849
38
KaurH.PrakashA.MedhiB. (2013). Drug therapy in stroke: from preclinical to clinical studies.Pharmacology92324–334. 10.1159/000356320
39
KepplingerJ.BarlinnK.DeckertS.ScheibeM.BodechtelU.SchmittJ. (2016). Safety and efficacy of thrombolysis in telestroke: a systematic review and meta-analysis.Neurology871344–1351. 10.1212/wnl.0000000000003148
40
KoganezawaN.HanamuraK.SekinoY.ShiraoT. (2017). The role of drebrin in dendritic spines.Mol. Cell. Neurosci.8485–92. 10.1016/j.mcn.2017.01.004
41
KonietznyA.BarJ.MikhaylovaM. (2017). Dendritic actin cytoskeleton: structure, functions, and regulations.Front. Cell Neurosci.11:147. 10.3389/fncel.2017.00147
42
KorobovaF.SvitkinaT. (2008). Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells.Mol. Biol. Cell191561–1574. 10.1091/mbc.E07-09-0964
43
KreisP.GallreinC.Rojas-PuenteE.MackT. G. A.KroonC.DinkelV.et al (2019). ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans.Nat. Commun.10:486. 10.1038/s41467-019-08420-w
44
KurisuK.YouJ.ZhengZ.WonS. J.SwansonR. A.YenariM. A. (2019). Cofilin-actin rod formation in experimental stroke is attenuated by therapeutic hypothermia and overexpression of the inducible 70 kD inducible heat shock protein (Hsp70).Brain Circ.5225–233. 10.4103/bc.bc_52_19
45
LaiT. W.ZhangS.WangY. T. (2014). Excitotoxicity and stroke: identifying novel targets for neuroprotection.Prog. Neurobiol.115157–188. 10.1016/j.pneurobio.2013.11.006
46
LanghorneP.CouparF.PollockA. (2009). Motor recovery after stroke: a systematic review.Lancet Neurol.8741–754. 10.1016/s1474-4422(09)70150-4
47
LiebeskindD. S.JüttlerE.ShapovalovY.YeginA.LandenJ.JauchE. C. (2019). Cerebral edema associated with large hemispheric infarction.Stroke502619–2625. 10.1161/strokeaha.118.024766
48
LinD. J.CloutierA. M.ErlerK. S.CassidyJ. M.SniderS. B.RanfordJ.et al (2019). Corticospinal tract injury estimated from acute stroke imaging predicts upper extremity motor recovery after stroke.Stroke503569–3577. 10.1161/STROKEAHA.119.025898
49
LivakK. J.SchmittgenT. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.Methods25402–408. 10.1006/meth.2001.1262
50
LoE. H. (2008). A new penumbra: transitioning from injury into repair after stroke.Nat. Med.14497–500. 10.1038/nm1735
51
LongaE. Z.WeinsteinP. R.CarlsonS.CumminsR. (1989). Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke2084–91. 10.1161/01.str.20.1.84
52
LvX.RenS. Q.ZhangX. J.ShenZ.GhoshT.XianyuA.et al (2019). TBR2 coordinates neurogenesis expansion and precise microcircuit organization via Protocadherin 19 in the mammalian cortex.Nat. Commun.10:3946. 10.1038/s41467-019-11854-x
53
MaR.XieQ.LiY.ChenZ.RenM.ChenH.et al (2020). Animal models of cerebral ischemia: a review.Biomed. Pharmacother.131:110686. 10.1016/j.biopha.2020.110686
54
MadineniA.AlhadidiQ.ShahZ. A. (2016). Cofilin Inhibition restores neuronal cell death in oxygen-glucose deprivation model of ischemia.Mol. Neurobiol.53867–878. 10.1007/s12035-014-9056-3
55
MarF. M.BonniA.SousaM. M. (2014). Cell intrinsic control of axon regeneration.EMBO Rep.15254–263. 10.1002/embr.201337723
56
MinamideL. S.StrieglA. M.BoyleJ. A.MebergP. J.BamburgJ. R. (2000). Neurodegenerative stimuli induce persistent ADF/cofilin-actin rods that disrupt distal neurite function.Nat. Cell Biol.2628–636. 10.1038/35023579
57
NashK. M.SchieferI. T.ShahZ. A. (2018). Development of a reactive oxygen species-sensitive nitric oxide synthase inhibitor for the treatment of ischemic stroke.Free Radic. Biol. Med.115395–404. 10.1016/j.freeradbiomed.2017.12.027
58
NishimuraY.ItoT.SaavedraJ. M. (2000). Angiotensin II AT(1) blockade normalizes cerebrovascular autoregulation and reduces cerebral ischemia in spontaneously hypertensive rats.Stroke312478–2486. 10.1161/01.str.31.10.2478
59
NohJ. E.OhS. H.ParkI. H.SongJ. (2020). Intracerebral transplants of GMP-grade human umbilical cord-derived mesenchymal stromal cells effectively treat subacute-phase ischemic stroke in a rodent model.Front. Cell Neurosci.14:546659. 10.3389/fncel.2020.546659
60
ObrdlikA.PercipalleP. (2011). The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation.Nucleus272–79. 10.4161/nucl.2.1.14508
61
ParkkinenS.OrtegaF. J.KuptsovaK.HuttunenJ.TarkkaI.JolkkonenJ. (2013). Gait impairment in a rat model of focal cerebral ischemia.Stroke Res. Treat.2013:410972. 10.1155/2013/410972
62
PetersA. J.LiuH.KomiyamaT. (2017). Learning in the Rodent motor cortex.Annu. Rev. Neurosci.4077–97. 10.1146/annurev-neuro-072116-031407
63
PowersW. J.RabinsteinA. A.AckersonT.AdeoyeO. M.BambakidisN. C.BeckerK.et al (2018). Guidelines for the early management of patients with acute ischemic stroke: a guideline for healthcare professionals from the American heart association/American stroke association.Stroke49e46–e99. 10.1161/STR.0000000000000158
64
Sanchez-BezanillaS.NilssonM.WalkerF. R.OngL. K. (2019). Can we use 2,3,5-triphenyltetrazolium chloride-stained brain slices for other purposes? The application of Western Blotting.Front. Mol. Neurosci.12:181. 10.3389/fnmol.2019.00181
65
Sanchez-VaroR.Trujillo-EstradaL.Sanchez-MejiasE.TorresM.Baglietto-VargasD.Moreno-GonzalezI.et al (2012). Abnormal accumulation of autophagic vesicles correlates with axonal and synaptic pathology in young Alzheimer’s mice hippocampus.Acta Neuropathol.12353–70. 10.1007/s00401-011-0896-x
66
SchafferA. E.BreussM. W.CaglayanA. O.Al-SanaaN.Al-AbdulwahedH. Y.KaymakçalanH.et al (2018). Biallelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration.Nat. Genet.501093–1101. 10.1038/s41588-018-0166-0
67
SekinoY.KojimaN.ShiraoT. (2007). Role of actin cytoskeleton in dendritic spine morphogenesis.Neurochem. Int.5192–104. 10.1016/j.neuint.2007.04.029
68
ShahK.RossieS. (2018). Tale of the good and the bad Cdk5: remodeling of the actin cytoskeleton in the brain.Mol. Neurobiol.553426–3438. 10.1007/s12035-017-0525-3
69
ShawA. E.BamburgJ. R. (2017). Peptide regulation of cofilin activity in the CNS: a novel therapeutic approach for treatment of multiple neurological disorders.Pharmacol. Ther.17517–27. 10.1016/j.pharmthera.2017.02.031
70
ShiraoT.HanamuraK.KoganezawaN.IshizukaY.YamazakiH.SekinoY. (2017). The role of drebrin in neurons.J. Neurochem.141819–834. 10.1111/jnc.13988
71
ShuL.ChenB.ChenB.XuH.WangG.HuangY.et al (2019). Brain ischemic insult induces cofilin rod formation leading to synaptic dysfunction in neurons.J. Cereb. Blood Flow Metab.392181–2195. 10.1177/0271678X18785567
72
SinclairL. I.TaylerH. M.LoveS. (2015). Synaptic protein levels altered in vascular dementia.Neuropathol. Appl. Neurobiol.41533–543. 10.1111/nan.12215
73
SorrellsS. F.ParedesM. F.Cebrian-SillaA.SandovalK.QiD.KelleyK. W.et al (2018). Human hippocampal neurogenesis drops sharply in children to undetectable levels in adults.Nature555377–381. 10.1038/nature25975
74
SpenceE. F.KanakD. J.CarlsonB. R.SoderlingS. H. (2016). The Arp2/3 complex is essential for distinct stages of spine synapse maturation, including synapse unsilencing.J. Neurosci.369696–9709. 10.1523/JNEUROSCI.0876-16.2016
75
StegnerD.HofmannS.SchuhmannM. K.KraftP.HerrmannA. M.PoppS.et al (2019). Loss of Orai2-mediated capacitative Ca(2+) entry is neuroprotective in acute ischemic stroke.Stroke503238–3245. 10.1161/strokeaha.119.025357
76
TanayE.MundelP.SommerC. (2006). Short-term ischemia usually used for ischemic preconditioning causes loss of dendritic integrity after long-term survival in the gerbil hippocampus.Brain Res.1112222–226. 10.1016/j.brainres.2006.07.004
77
ThorénM.DixitA.Escudero-MartínezI.GdovinováZ.KleckaL.RandV. M.et al (2020). Effect of recanalization on cerebral edema in ischemic stroke treated with thrombolysis and/or endovascular therapy.Stroke51216–223. 10.1161/strokeaha.119.026692
78
TonnesenJ.NagerlU. V. (2016). Dendritic spines as tunable regulators of synaptic signals.Front. Psychiatry7:101. 10.3389/fpsyt.2016.00101
79
VeutheyT. L.DerosierK.KondapavulurS.GangulyK. (2020). Single-trial cross-area neural population dynamics during long-term skill learning.Nat. Commun.11:4057. 10.1038/s41467-020-17902-1
80
ViraniS. S.AlonsoA.BenjaminE. J.BittencourtM. S.CallawayC. W.CarsonA. P.et al (2020). Heart disease and stroke statistics-2020 update: a report from the American heart association.Circulation141e139–e596. 10.1161/CIR.0000000000000757
81
Vivanco-HidalgoR. M.RiberaA.AbilleiraS. (2019). Association of socioeconomic status with ischemic stroke survival.Stroke503400–3407. 10.1161/STROKEAHA.119.026607
82
WangC.HuangR.LiC.LuM.EmanueleM.ZhangZ. G.et al (2019). Vepoloxamer enhances fibrinolysis of tPA (Tissue-Type Plasminogen Activator) on acute ischemic stroke.Stroke503600–3608. 10.1161/STROKEAHA.119.026049
83
WangH. J.ChenS. F.LoW. Y. (2016). Identification of Cofilin-1 induces G0/G1 arrest and autophagy in angiotensin-(1-7)-treated human aortic endothelial cells from iTRAQ quantitative proteomics.Sci. Rep.6:35372. 10.1038/srep35372
84
WangW. J.ZhongY. B.ZhaoJ. J.RenM.ZhangS. C.XuM. S.et al (2021). Transcranial pulse current stimulation improves the locomotor function in a rat model of stroke.Neural Regen. Res.161229–1234. 10.4103/1673-5374.301018
85
WangY.LiZ.WangY.ZhaoX.LiuL.YangX.et al (2018). Chinese stroke center alliance: a national effort to improve healthcare quality for acute stroke and transient ischaemic attack: rationale, design and preliminary findings.Stroke Vasc. Neurol.3256–262. 10.1136/svn-2018-000154
86
WangY. J.LiZ. X.GuH. Q.ZhaiY.JiangY.ZhaoX. Q.et al (2020). China stroke statistics 2019: a report from the National center for healthcare quality management in neurological diseases, China National clinical research center for neurological diseases, the chinese stroke association, National center for chronic and non-communicable disease control and prevention, Chinese Center for disease control and prevention and institute for global neuroscience and stroke collaborations.Stroke Vasc. Neurol.5211–239. 10.1136/svn-2020-000457
87
WonS. J.MinnellaA. M.WuL.EunC. H.RomeE.HersonP. S.et al (2018). Cofilin-actin rod formation in neuronal processes after brain ischemia.PLoS One13:e0198709. 10.1371/journal.pone.0198709
88
WuZ.SunF.LiZ.LiuM.TianX.GuoD.et al (2020). Electrical stimulation of the lateral cerebellar nucleus promotes neurogenesis in rats after motor cortical ischemia.Sci. Rep.10:16563. 10.1038/s41598-020-73332-5
89
XuM. S.ZhangS. J.ZhaoD.LiuC. Y.LiC. Z.ChenC. Y.et al (2013). Electroacupuncture-induced neuroprotection against cerebral ischemia in rats: role of the dopamine D2 receptor.Evid. Based Complement. Alternat. Med.2013:137631. 10.1155/2013/137631
90
YamadaR.KubaH. (2016). Structural and functional plasticity at the axon initial segment.Front. Cell Neurosci.10:250. 10.3389/fncel.2016.00250
91
YanW. J.DongH. L.XiongL. Z. (2013). The protective roles of autophagy in ischemic preconditioning.Acta Pharmacol. Sin.34636–643. 10.1038/aps.2013.18
92
YangY.LiQ.MiyashitaH.HowlettW.SiddiquiM.ShuaibA. (2000). Usefulness of postischemic thrombolysis with or without neuroprotection in a focal embolic model of cerebral ischemia.J. Neurosurg.92841–847. 10.3171/jns.2000.92.5.0841
93
YinL. M.JiangG. H.WangY.WangY.LiuY. Y.JinW. R.et al (2009). Use of serial analysis of gene expression to reveal the specific regulation of gene expression profile in asthmatic rats treated by acupuncture.J. Biomed. Sci.16:46. 10.1186/1423-0127-16-46
94
ZhouZ.LuJ.LiuW. W.ManaenkoA.HouX.MeiQ.et al (2018). Advances in stroke pharmacology.Pharmacol. Ther.19123–42. 10.1016/j.pharmthera.2018.05.012
Summary
Keywords
cerebral ischemia-reperfusion, gait analysis, cofilin-1, motor cortex, penumbra
Citation
Xu M-S, Yin L-M, Cheng A-F, Zhang Y-J, Zhang D, Tao M-M, Deng Y-Y, Ge L-B and Shan C-L (2021) Cerebral Ischemia-Reperfusion Is Associated With Upregulation of Cofilin-1 in the Motor Cortex. Front. Cell Dev. Biol. 9:634347. doi: 10.3389/fcell.2021.634347
Received
27 November 2020
Accepted
18 February 2021
Published
11 March 2021
Volume
9 - 2021
Edited by
Chang-Duk Jun, School of Life Sciences, Gwangju Institute of Science and Technology, South Korea
Reviewed by
Lize Xiong, Shanghai Fourth People’s Hospital Affiliated to Tongji University School of Medicine, Shanghai, China; Hisham F. Bahmad, Mount Sinai Medical Center, United States; Zahoor A. Shah, University of Toledo, United States
Updates
Copyright
© 2021 Xu, Yin, Cheng, Zhang, Zhang, Tao, Deng, Ge and Shan.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Lin-Bao Ge, 13301727622@189.cnChun-Lei Shan, shanclhappy@163.com
This article was submitted to Cell Adhesion and Migration, a section of the journal Frontiers in Cell and Developmental Biology
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