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ORIGINAL RESEARCH article

Front. Cell Dev. Biol., 08 January 2021
Sec. Molecular and Cellular Pathology
This article is part of the Research Topic The Application of AI and Other Advanced Technology in Studying Eye Diseases and Visual Development View all 21 articles

The Metabolic Reprogramming of Frem2 Mutant Mice Embryos in Cryptophthalmos Development

\r\nXiayin Zhang&#x;Xiayin Zhang1†Ruixin Wang&#x;Ruixin Wang1†Ting Wang&#x;Ting Wang1†Xulin Zhang&#x;Xulin Zhang1†Meimei DongyeMeimei Dongye1Dongni WangDongni Wang1Jinghui WangJinghui Wang1Wangting LiWangting Li1Xiaohang WuXiaohang Wu1Duoru LinDuoru Lin1Haotian Lin,*Haotian Lin1,2*
  • 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • 2Center for Precision Medicine, Sun Yat-sen University, Guangzhou, China

Background: Cryptophthalmos is characterized by congenital ocular dysplasia with eyelid malformation. The pathogenicity of mutations in genes encoding components of the FRAS1/FREM protein complex is well established, but the underlying pathomechanisms of this disease are still unclear. In the previous study, we generated mice carrying Frem2R725X/R2156W compound heterozygous mutations using CRISPR/Cas9 and showed that these mice recapitulated the human cryptophthalmos phenotype.

Methods: In this study, we tracked changes in the metabolic profile of embryos and expression of metabolism-related genes in Frem2 mutant mice on E13.5 compared with wild-type mice. RNA sequencing (RNA-seq) was utilized to decipher the differentiated expression of genes associated with metabolism. Untargeted metabolomics and targeted metabolomics analyses were performed to detect and verify the shifts in the composition of the embryonic metabolome.

Results: Differentially expressed genes participating in amino acid metabolism and energy metabolism were observed by RNA-seq. Transcriptomic analysis suggests that 821 (39.89%) up-regulated genes and 320 (32.99%) down-regulated genes were involved in the metabolic process in the enriched GO terms. A total of 92 significantly different metabolites were identified including creatine, guanosine 5′-monophosphate, cytosine, cytidine 5′-monophosphate, adenine, and L-serine. Interestingly, major shifts related to ATP binding cassette transporters (ABC transporters) and the biosynthesis of amino acids in the composition of the embryonic metabolome were observed by KEGG metabolic analysis, indicating that these pathways could also be involved in the pathogenesis of cryptophthalmos.

Conclusion: We demonstrate that Frem2 mutant fetal mice have increased susceptibility to the disruption of eye morphogenesis in association with distinct transcriptomic and metabolomic signatures. Our findings suggest that the metabolomic signature established before birth may play a role in mediating cryptophthalmos in Frem2 mutant mice, which may have important implications for the pathogenesis of cryptophthalmos.

Introduction

Cryptophthalmos (MIM: 123570) is a rare congenital ocular dysplasia accompanied by fusion of the eyelids (François, 1969). Mutations in the FREM2 gene result in reduced epithelial-mesenchymal coupling and transient embryonic epidermal blistering, leading to the development of cryptophthalmos (Jadeja et al., 2005; Haelst et al., 2010). However, the underlying pathomechanisms of this disease are still not known.

Metabolomics is considered as a sensitive approach for the development of novel targeted therapeutics because of its direct elucidation of pathophysiological mechanisms and its mediation as signals that directly or indirectly trigger adaptive responses (Piazza et al., 2018). Nutritional states, stress, and ecological conditions can all influence the intracellular levels of metabolites, and the development of the eye has been proven to be associated with intermediates of multiple metabolites by accumulating evidence (Robciuc et al., 2013; Cherif et al., 2018; Paramita et al., 2018). It is unclear whether the absence of metabolites drives epithelial–mesenchymal coupling in either mice or humans and whether the metabolic disorder is a consequence of cryptophthalmos.

Our objective, therefore, was to define the contribution of the metabolic disorder in driving cryptophthalmos as a predisposing factor. In our previous study, we established a mouse model that recapitulates the human complete cryptophthalmos phenotype characterized by abnormal eyelids, microphthalmia, and severe ocular anterior segment developmental defects (Zhang et al., 2019). Here, we used untargeted metabolomics analysis in conjunction with RNA-seq, to examine the contribution of metabolites and metabolism-related genes in modulating cryptophthalmos susceptibility in Frem2 mutant fetal mice. Real-time quantitative PCR and targeted metabolomics analysis were performed to verify the shifts in the composition of the embryonic metabolome. We suggest that the metabolites established before birth may have an impact on cryptophthalmos susceptibility in Frem2 mutant mice.

Materials and Methods

Animals

All animals used in this study were Mus musculus, C57BL/6J mice. All mouse procedures were approved by the Institutional Animal Care and Use Committee of Zhongshan Ophthalmic Center, Sun Yat-sen University. All experiments were carried out in accordance with the ARVO Statement for the Use and Care of Animals in Ophthalmic and Vision Research. The mice were maintained on a 12-h: 12-h light-dark cycle with unlimited access to food and water. All animals used for experiments were killed humanely and quickly by cervical dislocation (adult mice) or decapitation (embryos).

The generation of mice carrying the c.2173C > T (R725X) or c.6466A > T (R2156W) point mutation in the murine Frem2 gene was designed as previously reported using CRISPR/Cas9 technology (Zhang et al., 2019). According to our previous study, the phenotypes exhibited by the Frem2R725X/R2156W adult mice were bilateral or unilateral cryptophthalmos. Furthermore, 8/11 mice exhibited a single kidney and 4/11 mice exhibited syndactyly (Zhang et al., 2019).

Histological Analysis

The embryos from Frem2 mutant and wild-type mice were fixed with 4% paraformaldehyde and subsequently embedded in paraffin for at least 24 h. Tissues were sectioned in a vertical pupillary optic nerve plane and stained with H&E.

RNA-Seq Analysis and Real-Time Quantitative PCR Analysis

We compared embryonic transcriptome changes between three Frem2 mutant mice and one wild-type littermates. Total RNA was extracted with TRIzol reagent (Invitrogen). The RNA-seq libraries were generated using Illumina TruSeq RNA Sample Preparation Kits. RNA-seq was performed using an Illumina HiSeq 2000 platform as previously reported (Robciuc et al., 2013). Genes with q ≤ 0.05 and | log2_ratio| ≥ 1 were identified as differentially expressed genes. GO enrichment analyses of the differentially expressed genes were performed using DAVID.1 GO terms with a Bonferroni corrected P value of < 0.05 were considered significantly enriched functional annotations.

Real-time quantitative PCR was performed in three Frem2 mutant mice embryos and three wild-type embryos on a 7900HT Real-time PCR system (Applied Biosystems) using real-time primers and TaqMan probes from Applied Biosystems. The first strand was reverse-transcribed using the Omniscript Reverse Transcription Kit (Qiagen) and random primers. The fold changes in gene expression were calculated using the ΔΔCt (cycle threshold) values. Expression was normalized to Actin. All primers used are listed in Supplementary Table S1.

Untargeted Metabolomics Analysis

Untargeted high-throughput metabolomic profiling was conducted in six embryos of Frem2 mutant mice and compared with that in six wild-type embryos. Frozen embryos of Frem2R725X/R2156W and wild-type mice were thawed and analyzed using an UHPLC (1290 Infinity LC, Agilent Technologies) coupled with an AB SCIEX Triple TOF 6600 System (AB SCIEX, Framingham, MA, United States). In parallel to the preparation of the test samples (in a group of six), pooled quality control samples were prepared by mixing equal amounts (30 μL) of each sample. The quality control samples were utilized to monitor the LC-MS response in real time.

All samples were analyzed using a 2.1 mm × 100 mm ACQUIY UPLC BEH 1.7 μm column (Waters, Ireland). In both ESI positive and negative modes, the mobile phase contained the following: A, 25 mM ammonium acetate, and 25 mM ammonium hydroxide in water; B, acetonitrile (Merck, 1,499,230–935). The gradient elution program was 95% B for 1 min, linearly reduced to 65% in 13 min, reduced to 40% in 2 min, kept for 2 min, and then increased to 85% in 0.1 min, with a 5 min re-equilibration.

The ESI source conditions were set as follows: ion source gas1, 60; ion source gas2, 60; curtain gas, 30; source temperature, 600°C, ion spray voltage floating ± 5500 V, TOF MS scan m/z range, 60–1000 Da; and accumulation time, 0.20 s/spectra; production scan m/z range, 25–1000 Da; and accumulation time, 0.05 s/spectra for MS/MS acquisition. The production scan is acquired using information-dependent acquisition by high sensitivity mode with collision energy as 35 V with ±15 eV and declustering potential as ±60 V.

The raw MS data (wiff. scan files) were converted to mzXML files through ProteoWizard and processed by XCMS. The metabolites were identified by accuracy mass (<25 ppm) and MS/MS data that were matched with the lab database (Shanghai Applied Protein Technology Co., Ltd.). The variables having more than 50% of the valid values in at least one group were kept and handled for the multivariate statistical analysis by MetaboAnalyst2, as well as unidimensional statistical analysis by R software. The significantly different metabolites were determined based on the combination of a statistically significant threshold of VIP values obtained from supervised partial least squares-discriminate analysis and a two-tailed Student’s t-test. The VIP can measure the impact strength and interpretation capability of each metabolite pattern to distinguish samples in each group. Metabolites with VIP > 1.0 and P < 0.1 were considered significant. For metabolite annotation and pathway analysis, metabolites that showed differences between groups were analyzed by the KEGG3 database.

Targeted Metabolomics Analysis

Targeted high-throughput metabolomic profiling was conducted in five embryos of Frem2 mutant mice and compared with that in six wild-type mice. To precipitate proteins, the samples were incubated for 1 h at −20°C, followed by 15 min centrifugation at 13,000 r/min and 4°C. The resulting supernatant was removed and evaporated to dryness in a vacuum concentrator. The dry extracts were then reconstituted in 100 mL ACN: H2O (1:1, v/v), sonicated for 10 min, and centrifuged for 15 min at 13,000 r/min and 4°C to remove insoluble debris. The supernatants were transferred to HPLC vials for LC-MS analysis. The LC-MS analysis was performed using a UHPLC system (Agilent Technologies) coupled to a triple quadrupole mass spectrometer in the MRM mode. For the argininosuccinate and arginine tests alone, metabolites were monitored in positive mode only. For high-throughput metabolic profiling, metabolites were monitored in both ESI positive and ESI negative modes. A WATERS ACQUITY UPLC BEH Amino column (particle size, l.7 mm, 100 mm length × 2.1 mm ID) was used for argininosuccinate and arginine tests. Phenomenex Luna amino column (particle size, 3 mm; 100 mm length 3 2.1 mm ID) was used for metabolic profiling. The column temperature was kept at 25°C. The flow rate was 300 mL/min and the sample injection volume was 2 mL. Mobile phase A was 25 mM ammonium acetate and 25 mM ammonium hydroxide in 100% water, and B was 100% acetonitrile. The linear gradient for argininosuccinate and arginine tests was set as follows: 0–1 min: 85% B, 1–6 min: 85% B to 70% B, 6–10 min: 70% B to 0% B, 10–15 min: 0% B, 15–15.1 min: 0% B to 85% B, 15.1–20 min: 85% B. The linear gradient for metabolic profiling was set as follows: 0–1 min: 85% B, 1–14 min: 95% B to 65% B, 14–16 min: 65% B to 40% B, 16–18 min: 40% B, 18–18.1 min: 40% B to 95% B, 18.1–23 min: 95% B. ESI source conditions were set as follows: sheath gas temperature, 350°C; dry gas temperature, 350°C; sheath gas flow, 12 L/min for argininosuccinate and arginine tests, 11 L/min for metabolic profiling; dry gas flow, 16 L/min for argininosuccinate and arginine tests, 10 L/min for metabolic profiling; capillary voltage, 3000 V in positive mode for argininosuccinate and arginine tests, 4000 or −3500 V in positive or negative modes for metabolic profiling; nozzle voltage, 1000 V for argininosuccinate and arginine tests, 500 V for metabolic profiling; nebulizer pressure, 40 psi for argininosuccinate and arginine tests, 30 psi for metabolic profiling. For argininosuccinate and arginine tests, four MRM transitions representing the two metabolites were simultaneously monitored. The dwell time for each MRM transition is 50 ms, and the total cycle time is 535 ms. For metabolic profiling, the dwell time for each MRM transition is 3 ms, and the total cycle time is 1.263 s. Original MRM raw data were processed by MRMAnalyzer based on detection and area integration of peaks from individual target metabolites. Protein concentration was used for sample normalization.

To construct the metabolite MRM library, each metabolite standard (100 mg/mL) was first analyzed in ESI positive/negative mode via flow injection using the software MassHunter Optimizer (Agilent Technologies) to obtain the optimal MRM transition parameters. Then the retention time of each metabolite was determined by measuring the corresponding MRM transition individually on the column. The significantly different metabolites were determined based on the combination of a statistically significant threshold from fold change analysis (fold change > 1.5) and a two-tailed Student’s t-test (P < 0.05).

Statistical Analysis

The principal component analysis was performed to examine intrinsic clusters of metabolomics data. Heatmaps were generated using a hierarchical clustering algorithm to visualize metabolite differences. All statistical analyses were performed using SPSS (version 19.0) and R (version 3.3.1). Significance levels of P values are graphically represented with: for P values between 0.05 and 0.01.

Results

Ocular Abnormalities in Frem2 Mutant Embryos

As formation of the eyelids in the mouse begins at approximately 13–14 days of gestation (Pei and Rhodin, 1970), we studied E13.5 mice. H&E staining confirmed microphthalmia and showed dysplastic eye structures in the Frem2 mutant embryo (Figure 1). At the beginning of eyelid formation, the lower eyelid fold cannot be defined in the Frem2 mutant embryo, whereas small grooves developing from the surface ectoderm to form the eyelid folds above and below the developing eye have been well formed in wild-type mice. The corneal epithelium of the Frem2 mutant embryo stratifies unclearly with the reduction in corneal epithelial thickness. In addition, the dysplastic eyes exhibited a reduced axial length and reduced lens thickness compared to the wild-type mice.

FIGURE 1
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Figure 1. Abnormal ocular development in fetal Frem2 mutant mice. Histology of eyes from E13.5 Frem2R725X/R2156W mice (A,C) and wild-type mice (B,D). (A) The lower eyelid fold cannot be defined in Frem2 mutant mice, and the axial length and size of the lens are significantly reduced. (C) The corneal epithelium stratifies unclearly with the reduction in corneal epithelial thickness. The primary lens fibers are significantly shortened. Numbered arrows refer to the following: 1. eyelid; 2.cornea; 3. lens vesicle; 4. retina; 5. cornea epithelium; 6. lens epithelium; 7. primary lens fiber. Scale bars in all panels = 200 μm.

Identification of Differentially Expressed Genes Enriched in the Metabolic Process

To identify other genes that potentially contribute to the ocular phenotype, RNA-seq was performed with RNA from the whole embryos of E13.5 mice. RNA-seq results confirmed decreased expression of Frem2 and genes contributing to extracellular matrix organization and cell-matrix adhesion, including Ecm2, Col6a1, Col5a3, Colla1, and Lama5 in Frem2 mutant mice (Zhang et al., 2019). In addition, we observed differentially expressed genes including Stat5a, Cbs, Dpys, Adssl1, and Pm20d1 participating in amino acid metabolism, and Ldhb, Hk1, Chchd10 participating in energy metabolism in fetal Frem2R725X/R2156W mice.

Moreover, there were 821 (39.89%) up-regulated genes and 320 (32.99%) down-regulated genes involved in the metabolic process in the enriched GO terms of biological processes (Figure 2A). The decreased expressions of Ldhb and increased expression levels of Stat5a, Pm20d1, Chchd10, and Hk1 were verified by performing real-time quantitative PCR in the embryos of Frem2R725X/R2156W mice (Figure 2B).

FIGURE 2
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Figure 2. Differentially expressed genes enriched in the metabolic process. (A) A total of 821 (39.89%) up-regulated genes and 320 (32.99%) down-regulated genes involved in the metabolic process were identified in the enriched GO terms of biological processes. (B) The decreased expressions of Ldhb, and increased expression levels of Stat5a, Pm20d1, Chchd10, Hk1, Abcc3, Abcc9, and Abcc6 were verified by performing real-time quantitative PCR in the whole embryos of Frem2R725X/R2156W mice (*P < 0.05). Data are presented as the mean ± standard deviation (n = 3).

Frem2 Mutant Fetal Mice Have a Distinct Metabolomic Profile

To determine whether the metabolomic signatures were consistent with the transcriptomic signatures, a total of 92 metabolites that differed significantly between the mutant and wild-type embryos of E13.5 mice were identified by untargeted metabolomics analysis (Supplementary Table S2). Principal component analysis plots were performed for both positive and negative modes, showing that metabolite profiles in embryos from Frem2 mutant mice with ocular hypoplasia were very different from those in embryos from wild-type mice (Figures 3A,B). We identified the top 50 most different biologically significant metabolites, displayed by volcano plots in Figures 3C,D. Heatmaps of differentially expressed metabolites within fetal Frem2 mutant mice were visualized in Figure 4. Metabolites including creatine, guanosine 5′-monophosphate, cytosine, cytidine 5′-monophosphate, adenine, and L-serine were identified to be altered in the whole embryos of Frem2 mutant mice by targeted LC-MS analyses (Figure 3E).

FIGURE 3
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Figure 3. Metabolites profiles in fetal Frem2ΔR725X/R2156W mice. (A,B) Principal component analysis plots were performed under positive and negative modes. (C,D) Volcano plot showing the top 50 most different biologically significant metabolites under positive and negative modes. (E) Metabolites including creatine, guanosine 5′-monophosphate, cytosine, cytidine 5′-monophosphate, adenine, and L-serine were quantified in the embryos of Frem2 mutant mice compared with wild-type mice by targeted LC-MS analyses (*P < 0.05). (F) KEGG metabolic pathway analysis of different metabolites.

FIGURE 4
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Figure 4. Heatmap of differentially expressed metabolites within fetal Frem2ΔR725X/R2156W mice under (A) positive and (B) negative modes.

Metabolites determined in untargeted metabolomics analysis were screened and placed into the KEGG database to determine chemical and metabolic pathways that could be involved in the development of cryptophthalmos. A total of 153 possible pathways were found to be involved, covering 74 specific metabolites. KEGG metabolic pathway analysis of different metabolites annotated significant features related to ABC transporters and biosynthesis of amino acids, indicating that these substances could also be involved in the pathogenesis of cryptophthalmos (Table 1 and Figure 3F). Consistent with the KEGG analysis, real-time quantitative PCR confirmed the higher expression of ABC genes including Abcc3, Abcc6, and Abcc9, as well as genes contributing to the biosynthesis of amino acids in the embryos of Frem2R725X/R2156W mice (Figure 2B).

TABLE 1
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Table 1. The significantly altered pathways including more than ≥5 different metabolites.

Discussion

Ocular and eyelid development occurs in a step-wise fashion, and any misstep will be followed by the failure of subsequent steps (Tawfik et al., 2016). Our results confirmed that the phenotype of Frem2 mutant embryonic mice shows consistent results with that of the adult mice characterized by abnormal eyelids, microphthalmia, and developmental defects in the anterior segment. In addition, these congenital ocular dysplasias begin to appear at E13.5.

This study has highlighted the impact of loss-of-function mutations of Frem2 on the mouse embryonic metabolome. In particular, the data show connections between transcriptomic and metabolomic signatures in Frem2 mutant mice, which is intriguing coincides with reductions in metabolites participating in amino acid metabolism and energy metabolism. Metabolites identified to be altered in the Frem2 mutant mice by targeted LC-MS analyses, including guanosine 5′-monophosphate, cytosine, cytidine 5′-monophosphate, adenine, and L-serine, were all essential in the ocular development. Overall, we demonstrate that changes in metabolome composition may have contributed to the development of the cryptophthalmos phenotype in Frem2 mutant mice.

As shown in the KEGG metabolic pathway analysis, 16 different metabolites have been revealed to be related to the map of ABC transporters, which constitute a ubiquitous protein superfamily forming the largest transporter gene family expressed in various body tissues (Lee et al., 2016). ABC transporters translocate a wide variety of substrates, hydrophobic compounds, and metabolites across extra and intracellular membranes and play critical roles in maintaining intracellular balance (Gottesman et al., 2002; Szakacs et al., 2006). Mutations in these genes contribute to disorders including cystic fibrosis, neurological disease, retinal degeneration, cholesterol and bile transport defects, anemia, and drug response (Dean et al., 2001). Mutations in the ABCC6 gene were proved to be responsible for connective tissue disorder (Saux et al., 2000) and suggested to play a role in the transportation of toxic metabolites to which connective tissue cells are sensitive (Fung et al., 2019). In addition to these activities, ABC transporters have been shown to mediate epithelial to mesenchymal transition, and the cystic fibrosis transmembrane conductance regulator has been proven to function as a chloride/anion channel in epithelial cells around the body (Meng et al., 2016). However, it is still unclear to what extent this transport effect is related to epithelial–mesenchymal coupling and how FREM2 mutation affects ABC transporters.

Although connections between transcriptomic and metabolomic signatures have been shown in Frem2 mutant mice, the results of our study should be cautiously interpreted within the context of C57BL/6J mice embryos, as postnatal validation in mice and humans should be further confirmed. Additional experiments are also required to study whether the metabolic alterations were paralleled to the genetic changes or served as upstream indicators in cryptophthalmos development. Meanwhile, experiments designed to clarify the chronology of the pathogenesis of cryptophthalmos are also essential for identifying when defects in the formation of the eye and other tissues occur.

Shifts in metabolome composition and functionality may have contributed to the cryptophthalmos phenotype. As a result, we should acknowledge the influence of both direct mutations in genes and indirect metabolic programming effects caused by their modulation in utero. This is suggestive of a cooperative action utilizing both metabolic detection and screening of pathogenic genes in prenatal diagnostic testing, as cryptothalmos is difficult to diagnose by prenatal B-ultrasound. Furthermore, as novel therapeutics of genome editing tools enter the market, it becomes increasingly important to consider their effects on the metabolome.

Summary Statement

Metabolomic and transcriptomic signatures provide a map of metabolism in the Frem2 mutant mouse model of cryptophthalmos. These data provide insights into further mechanistic studies of the pathogenesis of cryptophthalmos.

Data Availability Statement

The data that support the findings of this study is available from the corresponding author upon reasonable request. The detected transcriptomics dataset and metabolomics datasets are available through Mendeley (https://data.mendeley.com/datasets/xfhsyssgrk/1).

Ethics Statement

All mouse procedures were approved by the Institutional Animal Care and Use Committee of Zhongshan Ophthalmic Center, Sun Yat-sen University.

Author Contributions

HL and XYZ designed the study. XYZ, RW, XLZ, DW, and MD performed the experiments. XYZ analyzed the data and wrote the manuscript. TW, XW, DL, JW, and WL critically revised the manuscript. All authors discussed the results and commented on the manuscript.

Funding

This study was funded by the National Natural Science Foundation of China (81770967 and 91546101). The funders had no role in the study design, data collection, data analysis, data interpretation, or writing of the report.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2020.625492/full#supplementary-material

The detected transcriptomics dataset and metabolomics datasets are available through Mendeley (https://data.mendeley.com/datasets/xfhsyssgrk/1).

Abbreviations

ABC transporters, ATP binding cassette transporters; ESI, electrospray ionization; H&E, hematoxylin-eosin; KEGG; Kyoto Encyclopedia of Genes and Genomes; LC-MS, liquid chromatography-mass spectrometry; MRM, multiple reaction monitoring; MS, mass spectrometry; PCR, polymerase chain reaction; RNA-seq, RNA sequencing; TOF, time-of-flight; UHPLC, ultra high-performance liquid chromatography; VIP, variable influence on projection.

Footnotes

  1. ^ http://david.abcc.ncifcrf.gov/
  2. ^ www.metaboanalyst.ca
  3. ^ http://www.genome.jp/kegg/

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Keywords: metabonomics, Frem2 mutation, cryptophthalmos, development of eyelids, transcriptomics

Citation: Zhang XY, Wang RX, Wang T, Zhang XL, Dongye MM, Wang DN, Wang JH, Li WT, Wu XH, Lin DR and Lin HT (2021) The Metabolic Reprogramming of Frem2 Mutant Mice Embryos in Cryptophthalmos Development. Front. Cell Dev. Biol. 8:625492. doi: 10.3389/fcell.2020.625492

Received: 03 November 2020; Accepted: 14 December 2020;
Published: 08 January 2021.

Edited by:

Zhiguo Chen, Capital Medical University, China

Reviewed by:

Zi-Bing Jin, Capital Medical University, China
Grace Zhai, University of Miami Miller School of Medicine, United States
Guoxing Xu, First Affiliated Hospital of Fujian Medical University, China

Copyright © 2021 Zhang, Wang, Wang, Zhang, Dongye, Wang, Wang, Li, Wu, Lin and Lin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Haotian Lin, linht5@mail.sysu.edu.cn; orcid.org/0000-0003-4672-9721

These authors have contributed equally to this work

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