Afarin Neishabouri1†
Alireza Soltani Khaboushan1,2†
Faezeh Daghigh3Abdol-Mohammad Kajbafzadeh1*
Masoumeh Majidi Zolbin1*- 1Pediatric Urology and Regenerative Medicine Research Center, Children’s Medical Center, Pediatric Center of Excellence, Tehran University of Medical Science, Tehran, Iran
- 2Students’ Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
- 3Department of Physiology, Faculty of Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran
Reproduction of different tissues using scaffolds and materials is a major element in regenerative medicine. The regeneration of whole organs with decellularized extracellular matrix (dECM) has remained a goal despite the use of these materials for different purposes. Recently, decellularization techniques have been widely used in producing scaffolds that are appropriate for regenerating damaged organs and may be able to overcome the shortage of donor organs. Decellularized ECM offers several advantages over synthetic compounds, including the preserved natural microenvironment features. Different decellularization methods have been developed, each of which is appropriate for removing cells from specific tissues under certain conditions. A variety of methods have been advanced for evaluating the decellularization process in terms of cell removal efficiency, tissue ultrastructure preservation, toxicity, biocompatibility, biodegradability, and mechanical resistance in order to enhance the efficacy of decellularization methods. Modification techniques improve the characteristics of decellularized scaffolds, making them available for the regeneration of damaged tissues. Moreover, modification of scaffolds makes them appropriate options for drug delivery, disease modeling, and improving stem cells growth and proliferation. However, considering different challenges in the way of decellularization methods and application of decellularized scaffolds, this field is constantly developing and progressively moving forward. This review has outlined recent decellularization and sterilization strategies, evaluation tests for efficient decellularization, materials processing, application, and challenges and future outlooks of decellularization in regenerative medicine and tissue engineering.
1 Introduction
Tissue engineering, as a division of regenerative medicine, combines engineering and biological science in order to reproduce tissues and organs that can help to overcome the lack of enough donor organs (Shafiee and Atala, 2017). Tissue engineering applies cells into desirable biological structures in a defined framework to restore the normal function of tissues. This process includes three cornerstones, namely scaffolds, cells, and signaling factors. As a critical component in tissue engineering, scaffolds provide mechanical stability and structural support for exogenous cell attachment and proliferation and facilitate the delivery of required growth factors for tissue regeneration (Vacanti and Langer, 1999; Soltani Khaboushan et al., 2021).
Scaffolds could be made up of natural tissues harvested from animal or human sources or being constructed using synthetic biomaterials. Natural scaffolds represent biological characteristics that better fit the regular tissue microenvironment, promoting appropriate cellular interactions, biocompatibility, and degradability. Decellularization is the process of eliminating cells and their components (especially DNA and RNA) from the extracellular matrix (ECM) to yield a natural matrix with saved mechanical integrity. It has been demonstrated that decellularized extracellular matrix (dECM) is a suitable type of natural scaffold for tissue engineering since the ECM plays a crucial role in tissue development (Gilbert et al., 2006).
ECM is mainly composed of water, proteins (mainly collagen), and polysaccharides (Frantz et al., 2010). The composition and arrangement of ECM components and microenvironmental conditions (e.g., mechanical properties, pH, CO2 concentration) of the matrix differ from one tissue to another based on the tissue function and its residing cells that secrete ECM components (Kawecki et al., 2018). Fibroblasts, adipocytes, and chondrocytes are among the cells involved in the formation of ECM components (including growth factors and structural proteins such as fibronectin). Physical, chemical, and biological methods have been utilized to produce acellular scaffolds, either by perfusion- or immersion/agitation-based systems, or even a combination of methods (Crapo et al., 2011).
So far, a wide variety of decellularization methods have been studied and developed (Crapo et al., 2011; Fu et al., 2014; Keane et al., 2015; Choudhury et al., 2020). Since acellular scaffolds have low immunogenicity and are biologically recognizable, they are beneficial for cell adhesion, proliferation, and survival. Because of these properties, decellularized materials have the potential to regenerate injured tissues or organs (Damodaran and Vermette, 2018). After decellularization, dECM should be sterilized, and several tests should assess the efficacy of decellularization. These tests are carried out to ensure the removal of cellular contents and preservation of biochemical and mechanical properties and include macroscopic and microscopic assessments, staining for evaluation of remaining components, and mechanical analyzes (Crapo et al., 2011). Moreover, these acellular scaffolds can be processed before application to acquire desired characteristics of the ECM for further use. Modifying and refining the dECM, recellularizing the scaffold, and providing a suitable biochemical and physical environment via bioreactors, could improve its characteristics and boost its regeneration ability in host tissues (Garreta et al., 2017).
The application of dECM as wound healing products and surgical mesh devices has been reported (Damodaran and Vermette, 2018; Daryabari et al., 2019; Uday Chandrika et al., 2021). In addition to therapeutic applications, dECM could be used for modeling various diseases, such as tumors, which helps to understand the pathophysiology and progression process of the disease (Liu et al., 2019; Pina et al., 2019). Despite the recent progress, tissue engineering is still in its early stages, and multiple studies are being conducted to develop functional organs. Up to the present, research was mainly directed towards discovering the ideal decellularization methods; and translational studies were performed to achieve functioning materials. This review aims to provide an overview of decellularization techniques, evaluation tests, dECM modification, practical approaches, clinical application, current insufficiencies, and future prospects in tissue decellularization.
2 Decellularization Agents and Methods
Several decellularization techniques have been developed to date to reconstruct different types of living organs. The main principle in all methods is removing cellular material and leaving the ECM ultrastructure unchanged in the tissue. Decellularization techniques differ in terms of applied materials (reagent combinations) and the routes used to deliver the main reagent, namely vascular, airway, or both (Uygun et al., 2010; Badylak et al., 2011; Crapo et al., 2011; Daryabari et al., 2019).
Generally, tissue decellularization methods are classified into three main groups: chemical methods, such as alkaline/acid, detergents, and alcohols; physical methods, such as electroporation, pressurization, freeze/thaw; biological methods, such as enzymes (Table 1) (Badylak et al., 2011; Gilpin and Yang, 2017). Several characteristics could sway the quality of the tissue decellularization process, including cell density, matrix thickness, and tissue morphology. These characteristics are different in various tissues; Thus, it is crucial to determine which method is the most suitable one for a specific tissue (Crapo et al., 2011; Heath, 2019).
TABLE 1. Decellularization methods and agents.
2.1 Chemical Agents
2.1.1 Ionic Detergents
Ionic detergents act by solubilizing DNA and cell membrane and tend to denature the proteins, thus decreasing collagen integrity. Ionic detergents are powerful in removing glycosaminoglycans (GAGs) and growth factors, therefore destroying ECM rigidity and function. Sodium dodecyl sulfate (SDS), sodium deoxycholate (SD), and Triton X-200 are ionic detergents that have been used in decellularization (Hudson et al., 2004; Gilbert et al., 2006; Lumpkins et al., 2008; Montoya and McFetridge, 2009; Zhou et al., 2010). Alshaikh et al. (Alshaikh et al., 2019) have examined SDS and SD in ovary decellularization. They have suggested a better ECM preservation in SD than SDS but lower donor DNA content in SDS. Due to polarity, ionic detergents such as SDS are hard to remove from ECM, and extensive wash with non-ionic detergents such as Triton X-100 is usually needed to take away the remnant ionic detergents from tissue (Gilpin and Yang, 2017).
2.1.2 Non-Ionic Detergents
Non-ionic detergents, such as Triton X-100, can strongly break lipid-lipid and lipid-protein bonds, but they are less effective on protein-protein interaction. Although they maintain the ultrastructure of the decellularized tissue and preserve the growth factors, they are less effective in removing cellular materials than SDS. The Triton X-100 is not appropriate for tissues where GAGs and lipids are important parts. Accordingly, the effectiveness of non-ionic detergents depends on the tissue undergoing the decellularization process (Gilbert et al., 2006; Lumpkins et al., 2008; Fu et al., 2010; Crapo et al., 2011; Gupta et al., 2018).
2.1.3 Zwitterionic Detergents
Zwitterionic detergents have the properties of both ionic and non-ionic detergents. They have shown better cell removal than non-ionic detergents and improved preservation of the ECM ultrastructure than ionic detergents (Hudson et al., 2004; Gupta et al., 2018; Heath, 2019). Zwitterionic detergents, include sulfobetaine-10 (SB-10), SB-16, Tri (n-butyl) phosphate (TnBP), and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS). CHAPS is a common zwitterionic agent that has been widely used in tissue decellularization (Tsuchiya et al., 2016; Gupta et al., 2018). TnBP is an organic solvent that dissociates protein-protein Interactions. TnBP was reported to match SDS in cell removal in tendon and ligament tissues (Deeken et al., 2011). Kuna et al. (Kuna et al., 2018) have reported that decellularization of the human saphenous vein using Triton X-100, TnBP, and deoxyribonuclease (DNase) resulted in properly removing the cells.
2.1.4 Chelators and Toxins
Ethylene glycol tetraacetic acid (EGTA) and ethylenediaminetetraacetic acid (EDTA) are used as chelating agents in organ decellularization through binding to divalent metal cations at the cell adhesion site of ECM. This binding was found to dissociate the cell from the remaining ECM. Due to their ability to damage cells, Cytotoxic agents could be used in the decellularization process (Crapo et al., 2011). Reyna et al. (Reyna et al., 2020) have shown the high efficiency of latrunculin, a toxin, in removing skeletal muscle cells by disrupting actin and myosin, thus damaging the cell and reducing DNA content to less than 10% compared to controls have shown the high efficiency of latrunculin, a toxin, in removing skeletal muscle cells by reducing DNA content to less than 10% compared to controls. They stated that this toxin is more effective in decellularizing skeletal muscle than ionic and non-ionic agent treatments.
2.1.5 Bases
Solutions with extreme pH were indicated to be effective in the decellularization process. Alkaline substances including sodium sulfide, ammonium hydroxide, calcium hydroxide, and sodium hydroxide were suggested as commonly used in organ decellularization processes. It has been shown that increasing the PH of CHAPS during decellularization increases the effectiveness of cell and protein removal. Bases could eliminate growth factors and disrupt the mechanical structure of the scaffold (Komai and Ushiki, 1991; Prasertsung et al., 2008; Choi et al., 2010; Reing et al., 2010; Sheridan et al., 2012; Tsuchiya et al., 2014; Gupta et al., 2018).
2.1.6 Acids
Acids were found to dissociate nuclear DNA from ECM by disrupting nucleic acids and solubilizing cytoplasmic components. Moreover, acids could facilitate the denaturation of the biomolecules. Acetic acid, hydrochloric acid, and sulfuric acid can disrupt cell membranes and be used in decellularization (Gilbert et al., 2006; Gupta et al., 2018). Lin et al. used and compared formic acid, acetic acid, and citric acid for porcine cornea decellularization (Lin et al., 2019). They showed that formic acid treatment had the optimal decellularizing effect and preserved in vitro and in vivo recellularization. Peracetic acid (PAA) can also be used as a decellularizing agent; however, it is mostly used as a sterilizing agent and has an unsuitable impact on decellularization. Kao and colleagues (Kao et al., 2020) have reported that the use of PAA in bladder decellularization was not successful because the levels of remaining DNA were similar to the native tissue.
2.1.7 Alcohols
Alcohols play their role in the decellularization process via dehydration; they diffuse into the cells, replace the intracellular water, disrupt the cells, and decrease their genetic materials. Alcohols such as methanol and ethanol are effective in lipid solubilization. Due to their role in tissue fixation and protein deposition, ethanol and methanol may affect the ultrastructure of the tissues. Lumpkin et al. have demonstrated that the use of acetone/ethanol in the decellularization of the temporomandibular joint disc came up with a stiffer tissue compared to Triton X-100 and SDS, and the mechanical characteristics of the decellularized tissue were not appropriately preserved (Lumpkins et al., 2008; Jamur and Oliver, 2010; Crapo et al., 2011).
2.1.8 Hypertonic and Hypotonic Solutions
Hypotonic and hypertonic solutions can lead to cell lysis and disruption of the DNA. Although they do not remove the cellular debris, they could improve decellularization in combination with other chemical reagents since they do not disturb ECM composition (Woods and Gratzer, 2005; Gupta et al., 2018).
2.2 Biological Enzymes
Enzymatic agents include phospholipase A2, proteases (e.g., trypsin, dispase), and nucleases (e.g., DNase, ribonuclease (RNase) are used in the decellularization process. Trypsin breaks peptides containing Lys and Arg, and in extended exposure, it may damage the ECM structure (Stenn et al., 1989; Gilbert et al., 2006; Rahman et al., 2018). Dispase chiefly cleaves fibronectin and collagen IV. It has been used in the decellularization of the porcine cornea and skin (Wilson et al., 2016; Joszko et al., 2019). Nucleases are mainly used in combination with other detergents to expedite the removal of DNAs and RNAs from the scaffold (Grauss et al., 2005; Heath, 2019). The application of phospholipase A2 in decellularization helps maintain collagen and proteoglycans in the tissue. It has been demonstrated that phospholipase A2, along with SD, was influential in producing the acellular porcine corneal stroma (Wu et al., 2009; Rahman et al., 2018).
2.3 Physical Methods
2.3.1 Freeze-Thaw Cycles
The freeze-thaw cycle is being done by fluctuation between freezing temperature (−80c°) and biological temperature. The freeze-thaw cycle disrupts the cell membranes and cell lysis via the formation of intracellular crystals. It has been demonstrated that this method considerably keeps the structure of the ECM but does not effectively remove the remnant cellular debris; thus, further detergents are needed after the freeze-thaw cycle. Multiple freeze-thaw cycles can be used in the decellularization process, whereas they could deteriorate the ECM structure (Pulver et al., 2014; Xing et al., 2015; Rahman et al., 2018).
2.3.2 Agitation Immersion and Pressure
The agitation in conjunction with immersion leads to cell lysis, but it is mostly used with chemical reagents to enhance the exposure of the ECM to the detergents and to augment the decellularization process. Agitation could be applied in the decellularization of the thin tissues, including the small intestine and bladder. In addition, the use of agitation and immersion in the decellularization of the tracheal tissue has been reported. The time of the protocol and intensity of agitation depends on the thickness of the tissue. Agitation may cause cell lysis before exposure to the detergents; thus, deteriorating the structure of the ECM. Furthermore, pressure could promote accessibility of the detergents to the ECM, hence decreasing the time of the decellularization process. Also, it can improve the removal of cellular remnant materials. The pressure leads to minimal changes in ECM structure, even less than agitation (Crapo et al., 2011; Heath, 2019; Rabbani et al., 2021). Sonication is another physical method and can be considered a subtype of agitation. It has been demonstrated that direct or indirect sonication increases agent penetration to the scaffold, therefore enhancing chemical decellularization while causing less damage to the ECM structural content (Forouzesh et al., 2019).
2.3.3 Supercritical Fluids
The supercritical fluids are over their critical pressure and temperature where it is not distinctly gas or liquid. Due to high permeability, these fluids could easily be removed from the tissue without the need for further washing. They can remove remnant cellular particles from the scaffold and decrease harmful changes in the ECM. Supercritical carbon dioxide has recently attracted attention in tissue decellularization because its critical temperature is appropriate for ECM processing. Recently, different studies reported complete effective removal of porcine skin via supercritical CO2 (Crapo et al., 2011; Chou et al., 2020; Rabbani et al., 2021). Supercritical CO2 not only decellularizes different tissues with better ECM structure preservation compared to conventional detergent-based techniques but also is used for sterilization of the dECM. Efficient cell removal and preserved ECM integrity were achieved by applying different supercritical CO2-based protocols on the optic nerve, myocardium, and cornea (Topuz et al., 2020).
3 Sterilization
Decontamination of the dECM via sterilization and disinfection process is necessary before further evaluations and in vitro or in vivo application. Sterilization kills all microorganisms, but the disinfection process only removes vegetative microorganisms and does not affect the bacterial spores. There are different sterilization methods that can be used for dECM methods. Appropriate sterilization and disinfection method should be selected considering various properties of the decellularized scaffold, including chemical and physical characteristics. Moreover, the needed time, availability, and target of application are important factors in choosing the proper method for sterilization (Kajbafzadeh et al., 2013; Fidalgo et al., 2018; Moradi et al., 2020a; Tao et al., 2021). Irradiation is a physical sterilization strategy that directly destroys nucleic acids and proteins from microorganisms. Gamma irradiation is extensively used for the sterilization of various tissues. Gamma irradiation can effectively sterilize corneal xenografts while preserving their structure and integrity. The irradiation has strong penetrance to the tissue without toxicity. Ultraviolet (UV) rays as a convenient physical disinfectant for the environment and surfaces are used for disinfecting thin decellularized scaffolds with a large surface. Large tissues such as decellularized kidneys cannot entirely be sterilized by UV, and it may interfere with further cell seeding on dECM (Islam et al., 2019; Moradi et al., 2020a; Gosztyla et al., 2020; Tao et al., 2021).
Ethylene oxide sterilizes the tissues by disrupting the function of the nucleic acids and proteins of the microorganisms. With its strong penetration, ethylene oxide can be used for sterilizing various decellularized scaffolds without causing toxicity. Peroxides are widely used as a disinfectant, which can also cause sterilization in specific conditions. They do not produce any toxic product after decomposition (Hennessy et al., 2017; Moradi et al., 2020a; Tao et al., 2021). According to a study, it has been demonstrated that PAA, as a peroxide, has the ability to decontaminate acellular rabbit kidney completely, whereas γ-irradiation destructed the structure tissue and UV failed to eliminate microorganisms (Moradi et al., 2020a). Alcohols are disinfectants that destroy the proteins within microorganisms, but they do not remove spores (Tao et al., 2021). They have minimal effects on the tissue structure; thus, they have been widely used to disinfect dECM derived from various tissues. Supercritical CO2, in addition to its effects on decellularization process, can lead to disinfection and sterilization of the decellularized scaffolds (Hennessy et al., 2017; Antons et al., 2018; Tao et al., 2021). CO2 laser, although less commonly used, has a great potential to be used for the sterilization process. CO2 laser bursts effectively removed bacteria inoculated on pig skin in an experiment. A sterile environment is a prerequisite of regenerative endodontic treatment, which could be effectively achieved with the assistance of a CO2 laser (Mullarky et al., 1985; Nammour and Majerus, 1991; Divya et al., 2021). Low-level laser with 660 nm wavelength and 100 mW power has been used for sterilization of the decellularized lung tissues (Lopes Guimarães et al., 2020). Laser is an appropriate option for effectively disinfecting tissues, and its potential for disinfecting the dECM should be further explored (Mullarky et al., 1985; Nammour and Majerus, 1991; Divya et al., 2021).
These methods may impact the structural and biochemical characteristics of the matrices. The γ-irradiation mainly affects the ultrastructure and mechanical properties of the scaffold, while ethanol and peracetic acid increase the ECM crosslinking (Hennessy et al., 2017; Johnson et al., 2017; Islam et al., 2019). In order to assess the efficacy of the sterilization method and its effects on the scaffold, several evaluations, including histological evaluations, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, mechanical tests, and bacterial and fungal cultures may be applied (Moradi et al., 2020b).
4 Evaluation of Decellularized Extracellular Matrix
Although decellularization techniques cannot remove 100% of cell material, quantitative analysis of cell components such as mitochondria, double-stranded DNA (dsDNA), and membrane-associated molecules (e.g., phospholipids) is a crucial way to ensure the effectiveness of the decellularization model. On the other hand, the ideal decellularization method should conserve biochemical and mechanical properties with the lowest toxicity rate for the subsequent recellularizing phase to occur. Various evaluation tests and assessments have been introduced to appraise the different characteristics of the dECM (Figure 1).
FIGURE 1. Evaluating the dECM. (A) Gross inspection of the decellularized ovine whole testes. (B) Hematoxylin and Eosin H&E staining of decellularized human breast skin, no nucleus can be observed. (C) 4’,6-diamidino-2-phenylindole (DAPI) staining of decellularized human skin with no stained DNA. (D) Masson’s Trichome staining of the human ovary, showing preserved collagen fibers while no nucleus is seen. (E) SEM imaging of decellularized ovine ovary tissue. (F) 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, showing cellular viability and activity where the purple dye is observed. (G) Immunohistochemistry (IHC) staining using antibodies against collagen I in human skin after recellularization. (H) Schematic imaging of the tensile test, tissue is placed in the middle of the apparatus and dragged in opposite directions to evaluate the mechanical strength of the dECM.
4.1 Macroscopic Evaluation
Despite the fact that gross inspection alone is not an entirely reliable evaluation method, it is the first step in the evaluation of the decellularized scaffold, which should be followed by more accurate assessments. Different studies use macroscopic imaging analysis as one of the first assessment steps to determine the level of transparency of dECM compared to native tissue (Philips et al., 2018a). Moreover, light transmittance is essential in several organ reconstructions, such as cornea (Oliveira et al., 2013). Transparency is measured by placing samples on a patterned surface. Then, the intensity is calculated by assessing the transmitted light by processing images with software (Oliveira et al., 2013; Philips et al., 2018a; Fernández-Pérez and Ahearne, 2019a).
4.2 Cell Removal Efficacy and Immunogenicity
Various tests assay the effectiveness of the decellularization method. Complete removal of antigens and nucleic acids, along with other cellular components, ensures the biocompatibility of the scaffold. The dECM must be implanted without host immune response activation and graft rejection or the formation of vascular thrombosis. Besides, transgenic transmission has been reported by retained DNA in genetically engineered tissue implantation (Hussein et al., 2016). Crapo et al. have suggested criteria for decellularization satisfaction with cut-off amounts for dsDNA per mg ECM dry weight and DNA fragment length, 50ng and 20bp, respectively, and lack of visible nuclear content in microscopic evaluation (Crapo et al., 2011). Also, extended agent exposure has been used to reduce DNA content by approximately 93% (Tenreiro et al., 2021). Histological analysis of the tissue allows evaluation of general morphology of dECM, as well as cell content and structural integrity (Philips et al., 2018a). Hematoxylin and Eosin (H&E) is mainly used in assessing tissue morphology and cell nuclei, while fluorescent staining with 4’,6-diamidino-2-phenylindole (DAPI) are used in the detection of remaining nuclear structures (Crapo et al., 2011; Su et al., 2018). Other histological staining such as Safrin O, Movat’s pentachrome, Masson’s Trichome, and Hoescht can also be used to evaluate the existence of remnant DNA or cytoplasmic and extracellular molecules in decellularized tissue (Garreta et al., 2017; Gaetani et al., 2018).
DNA quantification using immunofluorescence, electrophoresis, or polymerase chain reaction is also of great use (Pors et al., 2019; Naik et al., 2020; Hussein et al., 2018; da Mata Martins et al., 2020). Of note, electron microscopic assessment can be but is not commonly used to evaluate the existence of nuclear material or cytoplasmic debris due to the cost and the technical complexity (Gilbert et al., 2006).
4.3 Ultrastructure Evaluation
Preservation of 3 dimensional (3D) architecture and structure of the scaffold can be evaluated using different assessments, such as electron microscope (Giraldo-Gomez et al., 2019). A scanning electron microscope (SEM) is used to reveal surface topography, while transmission electron microscopy (TEM) is used for the detailed orientation of materials and cellular organelles (Márquez et al., 2009). These assessments are utilized to indicate the efficacy of decellularization treatment in conserving the native structure of ECM. In addition, they can show the debris and remaining cell components in the decellularized tissue (Philips et al., 2018a). In a study by Forouzesh et al., SEM images showed sonication-induced micropores with 0.5–5 μm diameter on the surface of decellularized cartilage tissue. Compared to the ultrasound-induced ones, these smaller micropores resulted in increased surface roughness, and suitable cell adhesion was imaged by SEM (Forouzesh et al., 2019).
4.4 Cytocompatibility
Prior to in vivo use, it is crucial to investigate the cellular interaction of cells with the decellularized scaffold. In other words, the damaging effects of the decellularization method or agent on the scaffolds should be assessed ex vivo to guarantee the clinical efficacy of the dECM. In this regard, the initial step is to quantify the remaining chemicals after washing and sterilizing. Decellularizing and sterilizing agents are cytotoxic and prevent efficient recellularization. The threshold for chemicals cytotoxic concentration, such as SDS has been investigated (Zvarova et al., 2016). The most valued biocompatibility test is in vitro cell culture, either indirect or direct (Hussein et al., 2016). In indirect contact assay, cellular proliferation (e.g., live/dead assay or DNA quantification) and metabolic activity (e.g., MTT assay) will be evaluated when cultured in samples extraction, while in direct contact assay, cells will be directly cultured on the decellularized samples and investigated.
4.5 Biochemical Analysis
Following decellularization, it is necessary to determine the remaining desirable ECM components such as GAGs, elastic fibers, collagens, and adhesion proteins like fibronectin and laminin in the decellularized tissue (Gilbert et al., 2006). An adequate amount of these molecules in ECM contributes to the tissue’s normal function, structure, and mechanical characteristics. Various stains, kits, and immunohistochemistry (IHC) markers can be used for the detection of these materials (Garreta et al., 2017; Kajbafzadeh et al., 2019). For instance, Masson’s trichrome is used for collagen fiber staining, and laminin can be detected by IHC antibodies (Kajbafzadeh et al., 2019).
4.6 Mechanical Tests
Following verification of complete cell removal, the impact of decellularization on the mechanical characteristics of ECM is also important. The ECM is composed of a network of molecules that confer a proper mechanical architecture in the tissue required to grow the desired cell population in tissue remodeling. It has been demonstrated that ECM elasticity plays a role in determining stem cell lineage specification, as matrices with elasticity similar to the brain, muscle, or collagenous bone induced neurogenic, myogenic, and osteogenic differentiation of mesenchymal stem cells (MSCs) (Engler et al., 2006). Atomic force microscopy (AFM), and single or bi-axial mechanical tests are vastly used to measure and compare the mechanical strength of the decellularized and native tissue. In AFM, samples are subjected to a probe, and indentation stress curves are obtained. Then these curves are analyzed, and AFC is measured and compared to native tissue (Peng et al., 2019). Moreover, mechanical testing, namely the burst pressure test, can provide insight into the existence, distribution, and integrity of collagen and elastin fibers within dECM (Bielli et al., 2018; Cai et al., 2019).
4.7 Additional Tests
Based on the purpose of the study and future function of the grafted dECM, researchers evaluate various components and characteristics of their acellular scaffold. Proteomic analysis of the dECM can precisely show and compare the proteome of the ECM before and after decellularization treatment. Proteomic evaluation can be exerted by different techniques, such as nano liquid chromatography and tandem mass spectrometry, and is used to assess the complete protein profile of the ECM, including remaining enzymes and growth factors (Nakayama et al., 2013). Computed tomography angiography is a technique to demonstrate remained vasculature of tissue after decellularization (Daryabari et al., 2019). In addition, during the process of hydrogel production, the ability of dECM to form gel could be analyzed by gelation assay of dECM to form a gel by gelation assay (Gaetani et al., 2018).
5 Pre-Application Processing
Before being applied, an acellular tissue may undergo a series of in vitro procedures, including cell seeding. Processing the scaffolds enhance their ability in successful grafting and function (García-Gareta et al., 2020; Mendibil et al., 2020) (Figure 2).
FIGURE 2. Pre-application processing of decellularized scaffolds. Modifying the decellularized scaffolds improves their capacity to regenerate damaged tissues without causing adverse events. In addition, processing scaffolds with stem cells and bioreactors helps improve their regeneration capacity.
5.1 Modification and Refinement
5.1.1 Vascularization
Oxygen delivery to the tissues is one of the hindrances against applying the decellularized scaffolds, and tissue thickness is an important index for this matter. Preserving the ECM components and vascular structure of the tissue yields better perfusion post-implantation, facilitating further angiogenesis. For instance, laminin and fibronectin in the basement membrane and lamina propria play an essential role in the revascularization of the tissue. Hence, evaluation of the scaffold components and structure after the decellularization process can help us estimate the extent of blood supply (Partington et al., 2013; García-Gareta et al., 2020). Vascularization can be induced by adding angiogenic factors to the matrix or prevascularization of the matrix (Amirsadeghi et al., 2020). Various growth factors were introduced to enhance angiogenesis. Drew et al. observed neovascularization in the presence of exogenous Vascular Endothelial Growth Factor (VEGF) (Dew et al., 2016). However, another study revealed that in bladder tissue regeneration, poly (octamethylene citrate) (POC) composites outperformed small intestinal submucosa (SIS) scaffolds in tissue vascularization, while POC scaffolds lack any exogenous growth factor (Bury et al., 2021). Stem cells are considered beneficial in angiogenesis induction, as they can differentiate into endothelial cells or secrete various growth factors. Adipose-derived stem cells (ADSCs) were reported to significantly enhance vascular network formation in decellularized adipose tissue scaffolds (Han et al., 2015). Prevascularization is a technique of creating microvasculature inside the synthetic scaffold; a decellularized tissue is already prevascularized. Whole organ matrices maintain the internal vasculature of the scaffold, bypassing the perfusion limits regardless of thickness and density (Wang et al., 2019). Hence, it is crucial to produce acellular organs with optimally preserved vessels and ECM composition for repopulating a capable scaffold and restoring blood circulation after implantation.
5.1.2 Immunogenicity Regulation
Non-autologous materials or cells on an engineered organ may trigger the immune response. Activation of the host immune system against synthetic, biological, and decellularized xenogeneic and allogeneic grafts has been reported as a hurdle in the transplantation of engineered tissues and organs (Wiles et al., 2016). Studies have proposed various approaches to overcome immune reaction against engineered tissue, including modulatory coatings on the scaffold surface, immune cloaking, modification of Damage Associated Molecular Pattern (DAMP) proteins which may remain after decellularization, and recellularization with autologous cells to suppress the immune response to materials (Wiles et al., 2016). Moreover, genetically engineered hypoimmunogenic stem cells are alternative to autologous cells to evade immune rejection (Deuse et al., 2019). One of the limitations concerning tissue decellularization is that most studies have examined these scaffolds on animal models, and limited human studies have been conducted so far. Consequently, translation of animal findings on the immune response to humans can be challenging. Human-like animals, such as nonhuman primates and humanized mouse models, with immune systems similar to humans, are being investigated to improve our understanding of the immunogenic components of the acellular scaffold (Bhattacharya et al., 2019; Bilodeau et al., 2020).
5.1.3 Bioprinting and Electrospinning
Bioprinting is a process through which bioinks are used to produce a 3D construct resembling human tissue that can be used to regenerate injured tissues and treat various diseases. Although bioprinting is progressively used in regenerative medicine to develop novel and effective treatments for various diseases, the lack of suitable bioinks hinders its widespread application in medicine (Ferreira et al., 2020; De Santis et al., 2021). Bioinks should be both cytocompatible and appropriate for structuring 3D tissues. 3D bioengineering of tissues using dECM bioink helps provide scaffolds for modeling diseases and developing efficient treatments for them. However, dECM needs augmentation to mechanically resemble the natural ECM (Ferreira et al., 2020; De Santis et al., 2021). Hydrogels could be made through solubilizing dECM followed by reformation of scaffold controlled by temperature and pH. Formation of dECM hydrogels is highly dependent on the collagen content of dECM. Hydrogel bioink derived from dECM can help deliver biomolecules and provide an appropriate surface for cells to migrate and grow (Saldin et al., 2017; Ferreira et al., 2020; De Santis et al., 2021). The biochemical, structural, and viscoelastic properties of dECM are tunable via combination with other biomaterials and crosslinking to provide maximum cytocompatibility along with structural support for tissues and cells. Alginate/dECM compositions as bioink demonstrated beneficial impacts on blood vessel formation after transplantation and prevention of foreign body reactions (Saldin et al., 2017; Ferreira et al., 2020; De Santis et al., 2021). Alginate reinforced with methacrylate-dECM demonstrated augmented bioactivity of hydrogel bioprinted scaffold and osteogenic differentiation of ADSCs (Lee et al., 2020).
Electrospinning has recently become increasingly popular for modifying the dECMs. Electrospinning may help design nanofibrous scaffolds from dECM with similar characteristics to the native tissue, including ultrastructure, porosity, and mechanical characteristics. Electrospun scaffolds demonstrated superior functionality and histocompatibility compared to scaffolds that are combined with other methods. Reinforcing rat decellularized vessels with Polycaprolactone (PCL) using the electrospinning method resulted in increased biomechanical endurance. Electrospinning provides an efficient method to combine natural and synthetic materials to achieve hybrid scaffolds that resemble native tissue with biomimetic architecture and enhanced biomechanical properties (Schenke-Layland et al., 2009; Kai et al., 2013; Gong et al., 2016).
5.1.4 Hybrid and Combined Scaffolds
Combining the decellularized scaffolds with other molecules, including biomaterials, drugs, and growth factors may help modify the characteristics of the decellularized scaffolds. The methods for designing combined scaffolds include solvent casting and particulate leaching, lyophilization (freeze-drying), thermal-induced phase separation (TIPS), gas foaming, rapid prototyping, stereolithography, fused deposition modeling, selective laser sintering, 3D printing, bioprinting, cross-linking, and electrospinning (Eltom et al., 2019; Wasyłeczko et al., 2020).
It was demonstrated that decellularized omentum scaffolds coated with gold (Au) nanoparticles (NP) could provide proper electrical conductivity and characteristics. Hence after seeding cardiac cells, the AuNP-decellularized scaffold can cause higher contraction force, decrease the excitability threshold, and increase the calcium channels currents. Also, silver (Ag) NPs show proangiogenic properties and enhance the biocompatibility of the scaffolds (Shevach et al., 2014; Saleh et al., 2019). When modified with the methoxy polyethylene glycol, an acellular adipose matrix decreases the immunogenicity and increases the adipogenicity of the scaffold (Liu et al., 2021a). Furthermore, polypropylene mesh coated with the ECM inhibits the M1 macrophages and increases the M1/M2 ratio (Wolf et al., 2014). Electrospun PCL blended with collagen in combination with decellularized rabbit aorta was found to provide biocompatibility and rigidity (Ghorbani et al., 2017). Scaffolds fabricated from dECM mixed with PCL have suitable properties for the growth and migration of stem cells. In addition, PCL/Poly Lactic-co-Glycolic Acid (PLGA) electrospun scaffold can support the cell-derived ECM to produce a dECM with desirable mechanical properties and biocompatibility (Schenke-Layland et al., 2009; Bracaglia and Fisher, 2015). Sugar-induced modification of the decellularized kidneys increases mechanical strength and resistance to deformation (Sant et al., 2021). Recently multilayered decellularized scaffolds demonstrated promising bioactivity and should be further studied to be widely used (Smith et al., 2022). Moreover, growth factors and drugs could be loaded on the dECMs, making them more potent. The addition of neurotrophic factors to dECM as well as removing chondroitin sulfate proteoglycans from dECM promote neurite outgrowth (Boyer et al., 2015; Qiu et al., 2020). Heparinized decellularized scaffolds have been examined for promoting angiogenesis and preventing clot formation (Wu et al., 2016). Layer-by-layer coating of the decellularized porcine aortic valve with basic fibroblast growth factor (bFGF) and heparin preserve a sustained release of these factors leading to improved biological activity (De Cock et al., 2010). Another study loaded fibroblast-derived ECM with VEGF and heparin to boost angiogenesis. Application of heparin/VEGF ECM encapsulated with alginate resulted in a prolonged release of these factors as well as enhanced bioactivity (Du et al., 2014). The dECMs are promising options for drug delivery; meanwhile, further studies are needed to increase their effectiveness.
5.1.5 Surface Modification
In order to achieve improved cytocompatibility, mechanical properties, and biological function of decellularized scaffolds without inflammatory reactions, the surface modification could be done via various methods and biomaterials (Ozasa et al., 2013; Liu et al., 2021b). It is demonstrated that immersion of decellularized tendons with carbodiimide-derivatized hyaluronic acid and gelatin could alleviate the tendon’s gliding resistance by augmenting its surface’s smoothness (Ozasa et al., 2013). Moreover, riboflavin-mediated UV crosslinking may repair the damages caused by the decellularization process and increase the smoothness and mechanical strength of the dECM; therefore, it could be used as the luminal surface in the vascular prosthesis (Schneider et al., 2020). Laser micro-ablation is another method that can produce microporosity in the scaffold’s surface (Matuska and McFetridge, 2018). In addition, coating the surface of the scaffolds using heparin reduces the thrombogenicity and makes their surface appropriate to be used as vascular grafts (Wu et al., 2016). Decellularized aorta covalently linked with heparin through “click” coating inhibited platelet adhesion and thrombogenicity. Also, Proper adhesion and proliferation of the endothelial cells were observed (Dimitrievska et al., 2015). Immobilization of VEGF on the surface of dECM through “click” reactions improves angiogenic properties of scaffolds (Wang et al., 2014). Accordingly, surface modification can optimize dECMs for various applications.
5.2 Recellularization
Acellular scaffolds have the capacity of in vitro or in vivo cell seeding. In situ tissue regeneration takes advantage of the recipient’s body’s regenerative capabilities and biological supply. Therefore, cell seeding of the scaffold prior to transplantation is not always necessary, but adding the bioactive molecules would reinforce the regeneration process (Yang et al., 2020). Thrombogenic and immunogenic factors on decellularized matrices may trigger thrombogenesis or the host immune response. These factors can be hidden, degraded, or modified by cells. Robertson et al. have shown that reendothelialization of whole heart ECM reduced the scaffold thrombogenicity (Robertson et al., 2014).
During the recellularization process, whole organ repopulation is difficult due to the complexity of the scaffold, the challenges of introducing cells to different parts of the scaffold, the need for the presence of different cell types, and uncertainty about the viability and functionality of cells after organ grafting (Bilodeau et al., 2020). However, advances in cell seeding techniques have provided solutions. Several strategies for the recellularization of a whole organ have been advocated, including slicing, perfusion, and injections (Figliuzzi et al., 2017). For instance, studies reported that the perfusion-based method is fruitful for the recellularization of acellular lungs and kidneys through the trachea and ureter, respectively (Song et al., 2013; Kuevda et al., 2016). Daryabari et al. implanted patches of the decellularized ovine whole uterus into uterine horns of female rats. They demonstrated regeneration of endometrium and myometrium layers, and vascularization was apparent (Daryabari et al., 2019). Whole organ recellularization through the vascular system on the liver, heart, pancreas, kidney, and lung with preservation of original architecture has been widely investigated (Gilpin et al., 2014a; Sabetkish et al., 2015; Scarrit et al., 2015; Ferng et al., 2017). Also, innovative techniques such as the exertion of negative trans-renal pressure gradient and magnetic guidance to direct cells have been proposed for repopulating complex tissues (Song et al., 2013; Ghodsizad et al., 2014).
Moreover, as an alternative method, intraparenchymal injection of the renal cell into the acellular porcine kidneys resulted in satisfactory repopulation and function (Abolbashari et al., 2016). Despite the progress in reseeding methods, slicing the organ demolishes its native 3D structure, the introduction of non-epithelial cells via perfusion is ineffective, and numerous cell injections may cause injuries to the scaffold (Figliuzzi et al., 2017). Therefore, multiple recellularization techniques might be employed to achieve optimal results in the meantime.
Although recellularization is considered a beneficial stage in the regeneration process, in vitro cell reseeding could be associated with some constraints. The recellularization process is time-consuming, especially when using induced pluripotent stem cells (iPSCs) derived from the patient’s somatic cells. One of the most important things to keep in mind is to utilize an adequate amount of the appropriate cell type. This factor varies in different tissues and organs and should be considered to reduce the risk of teratoma formation or immune response induction (Badylak et al., 2011; Kim et al., 2020). Multiple types of cells have been investigated for the recellularization of the scaffolds. Limited differentiation and expansion capacity of specialized cells restrains their effectiveness in recellularizing whole organ scaffolds. Stem cells have higher proliferation capacity and are mainly classified into adult stem cells, embryonic stem cells (ESCs), fetal stem cells, iPSCs, and other engineered cells (Denstedt and Atala, 2009; Bacakova et al., 2018). Mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) are the most common stem cells that have been used in the recellularization process. ESCs are pluripotent, but supply issues and ethical concerns restrain their application potential. The iPSCs are produced by the genetic reprogramming of adult somatic cells (Moser and Ott, 2014). Although iPSCs ensure histocompatibility and avoid ethical conflicts, tumor formation remains unresolved. Recently, iPSCs have been shown to produce self-organizing organoids with different cell types through genetic reprogramming or exposure to environmental signaling factors (Ebrahimkhani and Levin, 2021). Gilpin et al. evaluated the recellularization of decellularized lung scaffold with lung endothelial and epithelial progenitor cells derived from human iPSCs. They found decellularized lung scaffold could provide an appropriate environment for iPSC-derived progenitor cells (Gilpin et al., 2014b). Therefore, iPSCs seem promising in providing various functional cells for repopulating whole organs. MSCs are multipotent stem cells and can be easily harvested from various tissues such as bone marrow and adipose tissue. They can differentiate into several cell lineages and have demonstrated encouraging results in recellularizing different tissues. Thus, these cells have been used as a source for reseeding diverse tissues, such as cartilage (Zheng et al., 2011), respiratory tract (Hou et al., 2011; Mendez et al., 2014), urinary tract (Huang et al., 2007; Coutu et al., 2014), and cardiovascular system (Zhao et al., 2010).
It is preferred to use autologous cells for recellularizing acellular scaffolds since these cells would not trigger the host immune system reactions and rejection after transplantation. It has been demonstrated that reseeding acellular diaphragm and lung matrices with stromal cells from homogenized rat lung and diaphragm tissues had lower inflammation and fibrosis formation compared to reseeding with mesenchymal stem cells (Kuevda et al., 2019). In another study by Hellström and colleagues, primary uterine cells and bone marrow-derived mesenchymal stem cells (BM-MSCs) were seeded on rat decellularized uterine scaffolds (Hellström et al., 2016). They observed that these matrices were able to bear pregnancy and normal fetus development. The co-culture of parenchymal cells with non-parenchymal cells could enhance tissue development. Combining tissue-derived cells with endothelial cells or fibroblasts has been reported to improve ECM remodeling, cellular function, and cell organization (Badylak et al., 2011; Gilpin and Yang, 2017). Shen and colleagues have shown that endothelial cells are a critical component for neurogenesis of neural stem cells (Shen et al., 2004). Endothelial cells work as a barrier to prevent thrombosis and organ loss due to immunogenicity of the matrix after transplantation, especially in vascular and valvular grafts (Lichtenberg et al., 2006; Robertson et al., 2014). Furthermore, reendothelialization of whole decellularized heart vascular structures with rat aortic endothelial cells enhanced the contractility of left ventricular constructs (Robertson et al., 2014).
5.3 Bioreactors
Intercellular interactions and signaling play a major role in tissue development and function. Bioreactors provide a refreshing environment regarding factors, nutrients, and mechanical force that together enhance cell seeding (Martin et al., 2004). Mostly, bioreactors are perfusion-based systems involving a simple flow. Enhancement in the systems includes gravity involvement or providing a rotatory environment, optimization in oxygenation, or regulation of mechanical stimuli to mimic a natural microenvironment, such as compression and shear stress (Selden and Fuller, 2018). Spinning flasks, rotating cylindrical devices, perfusion bioreactors, and microfluidic systems are among the most used types of bioreactors (Ahmed et al., 2019). Unlike static culture conditions, bioreactors are able to monitor and control the environmental factors precisely. The incorporation of sensors into these devices allows detection of any changes, namely pH or concentration of factors (Simmons et al., 2017). The automation of these systems not only eliminates the need for manual and invasive balancing of the ECM and cellular interactions but also decreases manufacturing costs and enables inclusive clinical application (Martin et al., 2004).
6 Application of Decellularized Scaffolds in Regenerative Medicine
6.1 In Vitro Application
Decellularized scaffolds are being used for modeling the diseases to investigate their pathophysiology. Cell-cell communication, intracellular signaling, stem cell secretions, and therapeutics approaches could be studied in a 3D ex vivo scaffold to evaluate the role of the microenvironment in the progression of diseases, such as tumors and inflammatory conditions (García-Gareta et al., 2020). It has been shown that breast cancer cells had improved growth profiles when seeded on decellularized human adipose tissue compared to two-dimensional and Matrigel three-dimensional cultures (Dunne et al., 2014). In a recent study by Wishart and colleagues, the involvement of collagen-IV in breast cancer cell invasion was demonstrated (Wishart et al., 2020). Moreover, using decellularized human colorectal cancer matrices, Pinto et al. advocated that tumoral ECM macrophages enhanced cancer cell invasion via cytokine signaling (Pinto et al., 2017).
The underlying mechanism of the diseases and the effectiveness of the drugs could be assessed by modeling the diseases using decellularized scaffolds (McCrary et al., 2020a). For instance, acellular human intestine ECM was cultured with intestinal myofibroblasts to assess intestine fibrosis subsequent to inflammatory bowel disease (Giuffrida et al., 2019). Altered sensitivity of breast cancer cells to doxorubicin and lapatinib was also demonstrated in human adipose tissue-derived ECM scaffolds compared with 2D-cultured cells (Dunne et al., 2014). In another research, higher resistance to drug administration in cancer cells was reported in 3D cultures rather than 2D (Ganjibakhsh et al., 2019). Such studies support the beneficial aspects of in vitro application of decellularized tissue from normal or diseased tissues.
6.2 In Vivo Application
Since decellularized materials are biocompatible and stable compared to synthetic matrices, these cell-free scaffolds are being widely used (Kawecki et al., 2018). However, the attraction of desired stem cells to the site of the scaffold transplantation still remained challenging. Growth factors and chemoattractant substances could help overcome this pitfall (Yang et al., 2020). Sabetkish et al. used decellularized human testicles from patients with testicular feminization syndrome and implanted them between the thigh muscles of mice. Spermatogonial stem-like cells were observed during follow-up (Sabetkish et al., 2021).
Decellularized tissues, either cell-free or recellularized, primarily aim to function as implantable matrices for lost or injured organ regeneration (Figure 3). Multiple tissues have been introduced as candidates for tissue engineering, including the gastrointestinal tract, respiratory system, vascular, and neural tissues (Mendibil et al., 2020). Gilpin et al. have classified the applicable acellular scaffolds into cell sheets, tissues, and whole organs (Gilpin and Yang, 2017). Cell sheets are simple constructs mainly derived from a single cell type and may heal minor lesions or more complex structures when different sheets are combined. Cell-derived matrices are state-of-the-art ECM that can be used as an alternative to native tissue-derived matrices since human cells are accessible and limitless, and matrix properties can be controlled (Fitzpatrick and McDevitt, 2015).
FIGURE 3. Schematic diagram of preparation and application of the recellularized scaffolds. (A) The decellularized scaffolds could be obtained via chemical, biological, and physical approaches from human or animal sources. It has been demonstrated that stem cells from embryonic sources have a higher expansion capacity, albeit they are not easily accessible. Conversely, stem cells from adult sources, including mesenchymal stem cells, are highly available, whereas they do not have proliferation and differentiation capability as much as embryonic stem cells. Nevertheless, mesenchymal stem cells are multipotent stem cells that can differentiate into various cell lineages. The iPSC can be obtained via genetic modification of mature cells. Thus, they are highly expansible and readily available. (B) decellularized scaffolds can be used as cell-free grafts, or they can be seeded with stem cells. Decellularized scaffolds with or without stem cells could be transplanted for regeneration of the damaged tissues. Moreover, they are used in drug delivery, drug screening, disease modeling, and studying the pathophysiology of diseases.
It is evidenced that genetic modification may improve the characteristics of ECM and the efficacy of the decellularization process. Effective decellularization process could be reached via apoptosis of death-inducible stem cells. Also, genetic modification of the stem cells may help tune the ECM composition, such as modifying VEGF expression by stem cells (Papadimitropoulos et al., 2015; Bourgine et al., 2017; Morris et al., 2018). Acellular ECM derived from BM-MSC sheets was used to treat osteochondral defects in rabbits (Wang et al., 2020). These matrices promoted regeneration of articular cartilage and subchondral bone with some degrees of vascularization. Reconstruction of blood vessels, combination of various decellularized cartilage sheets to produce two-dimensional cartilage assembly, and recellularization of periodontal ligament cell sheets were also reported (Fitzpatrick et al., 2008; Gilpin and Yang, 2017).
Acellular ECM hydrogels can also provide a scaffold for transplantation and injection (Young et al., 2011; Wolf et al., 2012). In addition, transplantation of recellularized acellular scaffolds has been approached by many researchers. In a study, regenerated rat lungs were orthotopically transplanted to investigate the function of reendothelialized pulmonary vasculature (Tsuchiya et al., 2017). They showed blood perfusion in the transplanted lung as well as leak-free ventilation. Transplantation of reseeded decellularized diaphragm into rats resulted in improved spirometry parameters (Gubareva et al., 2016).
Currently, decellularized tissues are widely being studied for organ regeneration. Kajbafzadeh and colleagues have implanted acellular rat colon-derived scaffolds into the mesenteric tissue as a graft with an end-to-end anastomosis to the colon of host rats that resulted in recellularization of the scaffold (Kajbafzadeh et al., 2020). The trachea, heart valve, and urethra are examples that have been clinically regenerated with decellularized scaffolds (Versteegden et al., 2017; Porzionato et al., 2018).
6.3 Human Studies
Human application of different acellular organs is probably the ultimate goal of tissue engineering and regenerative medicine. Due to the shortcoming of living organs donation, decellularization and tissue engineering methods could pave the way for regenerating tissues and organs to treat diseases in a less invasive procedure.
Variability in human tissues and their immunogenicity makes it inconceivable to propose a standardized decellularization method, thus precluding acellular whole-organ transplantation (Tenreiro et al., 2021; Mattei et al., 2017). Other hindrances include ideal vascularization, innervation, or recellularization of acellular scaffolds (Choudhury et al., 2018). Application of cell-free grafts is a relatively new area of the clinical setting to use acellular matrices. Many commercial companies have been producing dECM, namely Biohorizon and Axogen, which produce acellular skin graft and dECM nerve scaffolds. In addition, many human organ-derived dECM-bioprinted hydrogels have been used in drug screening and delivery (Choudhury et al., 2020; Lopresti et al., 2015; Cui et al., 2019). Also, AlloDerm (derived from the allogenic human cadaveric dermis), Strattice (derived from porcine dermis), and OaSIS (derived from porcine SIS) are commercial cell-free products that have been used in recent decades mainly for covering the skin flaps donor sites, breast reconstruction, and managing ulcers and wounds, respectively (Cui et al., 2019). In a clinical trial, human acellular vessels showed safety and functionality in providing access for hemodialysis in end-stage renal disease patients (Lawson et al., 2016). Different decellularized tissues and their application status is reviewed by Liao et al. (Liao et al., 2020). Currently, several clinical trials in different phases are investigating the application of decellularized tissue in the regeneration of injured or lost organs. An active phase II study is being conducted at the Department of Biomedical Engineering, Johns Hopkins University, investigating acellular adipose tissue for soft tissue reconstruction (identifier: NCT03544632, clinicaltrials.gov). Various decellularized scaffolds from animal and human sources have been used clinically, mainly for wound healing and surgical mesh devices, albeit their application is still limited, and further studies are needed to make decellularized scaffolds commercially available treatment of disease (Damodaran and Vermette, 2018).
7 Discussion: Challenges and Future Directions
Despite favorable characteristics of the decellularized scaffolds for implementation in clinical practice, there are some constraints and challenges in their application. Determining the suitable tissue, decellularization and sterilization method selection, optimizing the efficacy of these techniques, dECM quality assessment, modification of acellular scaffolds in vitro and in vivo study, and transplantation problems (e.g., the timing of biomaterial transplantation) are among the unsolved challenges regarding the clinical translation of the decellularized scaffolds (Figure 4) (Zhang et al., 2021).
FIGURE 4. Challenges and hurdles regarding the decellularization process. The decellularization process encounters different challenges that may affect the resulting scaffold.
The decellularization method is selected based on the type of tissue and its characteristics. Developing cell removal techniques with lower toxic agents or productions, in addition to enhancing sterilizing techniques, would be beneficial. Apart from that, unlike most synthetic scaffolds (Han et al., 2015), purified ECM provides a natural microenvironment for the cells that is challenging to preserve its properties during the decellularization process (Frantz et al., 2010; Crapo et al., 2011). Ongoing research is aspiring to develop new techniques to better preserve the ECM architecture and components, such as vacuum-assisted and apoptosis-assisted decellularization (Zhang et al., 2021). Removing cells from ECM can become more efficient if an innate cellular process or an exogenous stimulus, such as a drug, promotes cell death, thereby eliminating possible ECM structural damage. Cartilage ECM was yielded by culturing human mesenchymal stromal cells with death-inducible genetic systems. This method resulted in more productive bone tissue engineering compared to cartilage ECM obtained from freeze-thaw cycles (Bourgine et al., 2014). Also, camptothecin-treated nerve tissue resulted in cellular apoptosis and clearance, which was immunogenically tolerable in vivo (Cornelison et al., 2018).
Various evaluation tests have already been established to ensure that cellular compounds have been cleared from the dECM during the decellularization treatment, while the chemical and biomechanical properties remain intact and the residual detergent is removed from the decellularized tissue, as the remaining detergents may cause cell toxicity in the scaffold (Zhang et al., 2021). Each tissue has unique characteristics that make it proper for a research plan and further clinical use. Additional novel analyzing tests should be developed for each tissue and its future point of function, letting researchers assess the acellular scaffold specifically and exclusively.
Application of naturally-derived ECM is restricted owing to limited animal sources that are free of by-products and contaminants, while synthetic scaffolds are available in ample amounts (Frantz et al., 2010). Polymerization of biodegradable materials is becoming increasingly popular since manufacturing techniques such as electrospinning can help create a large number of scaffolds with controllable indexes, including shapes, porosities, and fiber arrangements (Nam et al., 2007; Langer and Vacanti, 2016; Asghari et al., 2017). In order to overcome the damages caused by the decellularization process and take advantage of engineered materials, many researchers have attempted to modify decellularized scaffolds, print 3D scaffolds using bio-inks, or design ECM-derived hydrogels (García-Gareta et al., 2020). In terms of regenerative medicine, 3D printing is a valuable tissue fabrication technology since it is capable of reproducing the structure of the desired organ; however, clinical application of these 3D bioprinted constructs depends on further regulations and improvements (Ji and Guvendiren, 2017; Bilodeau et al., 2020).
Although the aforementioned strategies have been effective to a large extent in the decellularization process, there are still many hindrances in the endless road of dECM engineering. Despite all progress in this field, further investigations are needed to overcome challenges regarding the clinical application of decellularized biomaterials and the ultimate goal of whole organ transplantation.
Author Contributions
AN conceptualized the title, prepared the first draft, revised the manuscript, and designed the table and figures. ASK conceptualized the title, prepared the first draft, revised the manuscript, and designed the figures. FD conceptualized the title and prepared the first draft. MMZ conceptualized the title, critically revised the manuscript, edited and finalized the draft, supervised the project. A-MK conceptualized the title, critically revised the manuscript, edited and finalized the draft, and supervised the project. All the authors have read and approved the final draft of the manuscript.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Acknowledgments
Figure 1F, Figure 2, and Figure 3 have been created with BioRender.com.
References
Abolbashari, M., Agcaoili, S. M., Lee, M.-K., Ko, I. K., Aboushwareb, T., Jackson, J. D., et al. (2016). Repopulation of Porcine Kidney Scaffold Using Porcine Primary Renal Cells. Acta Biomater. 29, 52–61. doi:10.1016/j.actbio.2015.11.026
Ahmed, S., Chauhan, V. M., Ghaemmaghami, A. M., and Aylott, J. W. (2019). New Generation of Bioreactors that advance Extracellular Matrix Modelling and Tissue Engineering. Biotechnol. Lett. 41 (1), 1–25. doi:10.1007/s10529-018-2611-7
Alshaikh, A. B., Padma, A. M., Dehlin, M., Akouri, R., Song, M. J., Brännström, M., et al. (2019). Decellularization of the Mouse Ovary: Comparison of Different Scaffold Generation Protocols for Future Ovarian Bioengineering. J. Ovarian Res. 12 (1), 58–59. doi:10.1186/s13048-019-0531-3
Amirsadeghi, A., Jafari, A., Eggermont, L. J., Hashemi, S.-S., Bencherif, S. A., and Khorram, M. (2020). Vascularization Strategies for Skin Tissue Engineering. Biomater. Sci. 8 (15), 4073–4094. doi:10.1039/d0bm00266f
Antons, J., Marascio, M. G., Marascio, M., Aeberhard, P., Weissenberger, G., Hirt-Burri, N., et al. (2018). Decellularised Tissues Obtained by a CO2-philic Detergent and Supercritical CO2. eCM 36, 81–95. doi:10.22203/ecm.v036a07
Asghari, F., Samiei, M., Adibkia, K., Akbarzadeh, A., and Davaran, S. (2017). Biodegradable and Biocompatible Polymers for Tissue Engineering Application: a Review. Artif. Cell nanomedicine, Biotechnol. 45 (2), 185–192. doi:10.3109/21691401.2016.1146731
Assmann, A., Struß, M., Schiffer, F., Heidelberg, F., Munakata, H., Timchenko, E. V., et al. (2017). Improvement of the In Vivo Cellular Repopulation of Decellularized Cardiovascular Tissues by a Detergent-free, Non-proteolytic, Actin-Disassembling Regimen. J. Tissue Eng. Regen. Med. 11 (12), 3530–3543. doi:10.1002/term.2271
Bacakova, L., Zarubova, J., Travnickova, M., Musilkova, J., Pajorova, J., Slepicka, P., et al. (2018). Stem Cells: Their Source, Potency and Use in Regenerative Therapies with Focus on Adipose-Derived Stem Cells - a Review. Biotechnol. Adv. 36 (4), 1111–1126. doi:10.1016/j.biotechadv.2018.03.011
Badylak, S. F., Taylor, D., and Uygun, K. (2011). Whole-organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Scaffolds. Annu. Rev. Biomed. Eng. 13, 27–53. doi:10.1146/annurev-bioeng-071910-124743
Bhattacharya, R., Das, P., Joardar, S. N., Biswas, B. K., Batabyal, S., Das, P. K., et al. (2019). Novel Decellularized Animal Conchal Cartilage Graft for Application in Human Patient. J. Tissue Eng. Regen. Med. 13 (1), 46–57. doi:10.1002/term.2767
Bielli, A., Bernardini, R., Varvaras, D., Rossi, P., Di Blasi, G., Petrella, G., et al. (2018). Characterization of a New Decellularized Bovine Pericardial Biological Mesh: Structural and Mechanical Properties. J. Mech. Behav. Biomed. Mater. 78, 420–426. doi:10.1016/j.jmbbm.2017.12.003
Bilodeau, C., Goltsis, O., Rogers, I. M., and Post, M. (2020). Limitations of Recellularized Biological Scaffolds for Human Transplantation. J. Tissue Eng. Regen. Med. 14 (3), 521–538. doi:10.1002/term.3004
Bourgine, P. E., Scotti, C., Pigeot, S., Tchang, L. A., Todorov, A., and Martin, I. (2014). Osteoinductivity of Engineered Cartilaginous Templates Devitalized by Inducible Apoptosis. Proc. Natl. Acad. Sci. U.S.A. 111 (49), 17426–17431. doi:10.1073/pnas.1411975111
Bourgine, P. E., Gaudiello, E., Pippenger, B., Jaquiery, C., Klein, T., Pigeot, S., et al. (2017). Engineered Extracellular Matrices as Biomaterials of Tunable Composition and Function. Adv. Funct. Mater. 27 (7), 1605486. doi:10.1002/adfm.201605486
Boyer, R. B., Sexton, K. W., Rodriguez-Feo, C. L., Nookala, R., Pollins, A. C., Cardwell, N. L., et al. (2015). Adjuvant Neurotrophic Factors in Peripheral Nerve Repair with Chondroitin Sulfate Proteoglycan-Reduced Acellular Nerve Allografts. J. Surg. Res. 193 (2), 969–977. doi:10.1016/j.jss.2014.09.023
Bracaglia, L. G., and Fisher, J. P. (2015). Extracellular Matrix-Based Biohybrid Materials for Engineering Compliant, Matrix-Dense Tissues. Adv. Healthc. Mater. 4 (16), 2475–2487. doi:10.1002/adhm.201500236
Bury, M. I., Fuller, N. J., Sturm, R. M., Rabizadeh, R. R., Nolan, B. G., Barac, M., et al. (2021). The Effects of Bone Marrow Stem and Progenitor Cell Seeding on Urinary Bladder Tissue Regeneration. Sci. Rep. 11 (1), 1–12. doi:10.1038/s41598-021-81939-5
Cai, Z., Gu, Y., Cheng, J., Li, J., Xu, Z., Xing, Y., et al. (2019). Decellularization, Cross-Linking and Heparin Immobilization of Porcine Carotid Arteries for Tissue Engineering Vascular Grafts. Cell Tissue Bank 20 (4), 569–578. doi:10.1007/s10561-019-09792-5
Caralt, M., Uzarski, J. S., Iacob, S., Obergfell, K. P., Berg, N., Bijonowski, B. M., et al. (2015). Optimization and Critical Evaluation of Decellularization Strategies to Develop Renal Extracellular Matrix Scaffolds as Biological Templates for Organ Engineering and Transplantation. Am. J. Transplant. 15 (1), 64–75. doi:10.1111/ajt.12999
Cheng, J., Wang, C., and Gu, Y. (2019). Combination of Freeze-Thaw with Detergents: A Promising Approach to the Decellularization of Porcine Carotid Arteries. Bme 30 (2), 191–205. doi:10.3233/bme-191044
Choi, J. S., Williams, J. K., Greven, M., Walter, K. A., Laber, P. W., Khang, G., et al. (2010). Bioengineering Endothelialized Neo-Corneas Using Donor-Derived Corneal Endothelial Cells and Decellularized Corneal Stroma. Biomaterials 31 (26), 6738–6745. doi:10.1016/j.biomaterials.2010.05.020
Chou, P.-R., Lin, Y.-N., Wu, S.-H., Lin, S.-D., Srinivasan, P., Hsieh, D.-J., et al. (2020). Supercritical Carbon Dioxide-Decellularized Porcine Acellular Dermal Matrix Combined with Autologous Adipose-Derived Stem Cells: its Role in Accelerated Diabetic Wound Healing. Int. J. Med. Sci. 17 (3), 354–367. doi:10.7150/ijms.41155
Choudhury, D., Anand, S., and Naing, M. W. (2018). The Arrival of Commercial Bioprinters - towards 3D Bioprinting Revolution! Int. J. Bioprint 4 (2), 139. doi:10.18063/IJB.v4i2.139
Choudhury, D., Yee, M., Sheng, Z. L. J., Amirul, A., and Naing, M. W. (2020). Decellularization Systems and Devices: State-Of-The-Art Review. Acta Biomater. 115, 51–59. doi:10.1016/j.actbio.2020.07.060
Cornelison, R. C., Wellman, S. M., Park, J. H., Porvasnik, S. L., Song, Y. H., Wachs, R. A., et al. (2018). Development of an Apoptosis-Assisted Decellularization Method for Maximal Preservation of Nerve Tissue Structure. Acta Biomater. 77, 116–126. doi:10.1016/j.actbio.2018.07.009
Coutu, D. L., Mahfouz, W., Loutochin, O., Galipeau, J., and Corcos, J. (2014). Tissue Engineering of Rat Bladder Using Marrow-Derived Mesenchymal Stem Cells and Bladder Acellular Matrix. PLoS One 9 (12), e111966. doi:10.1371/journal.pone.0111966
Crapo, P. M., Gilbert, T. W., and Badylak, S. F. (2011). An Overview of Tissue and Whole Organ Decellularization Processes. Biomaterials 32 (12), 3233–3243. doi:10.1016/j.biomaterials.2011.01.057
Cui, H., Chai, Y., and Yu, Y. (2019). Progress in Developing Decellularized Bioscaffolds for Enhancing Skin Construction. J. Biomed. Mater. Res. A. 107 (8), 1849–1859. doi:10.1002/jbm.a.36688
da Mata Martins, T. M., da Silva Cunha, P., Rodrigues, M. A., de Carvalho, J. L., de Souza, J. E., de Carvalho Oliveira, J. A., et al. (2020). Epithelial Basement Membrane of Human Decellularized Cornea as a Suitable Substrate for Differentiation of Embryonic Stem Cells into Corneal Epithelial-like Cells. Mater. Sci. Eng. C 116, 111215. doi:10.1016/j.msec.2020.111215
Damodaran, R., and Vermette, P. (2018). Tissue and Organ Decellularization in Regenerative Medicine. Biotechnol. Prog. 34 (6), 1494–1505. doi:10.1002/btpr.2699
Daryabari, S. S., Kajbafzadeh, A.-M., Fendereski, K., Ghorbani, F., Dehnavi, M., Rostami, M., et al. (2019). Development of an Efficient Perfusion-Based Protocol for Whole-Organ Decellularization of the Ovine Uterus as a Human-Sized Model and In Vivo Application of the Bioscaffolds. J. Assist. Reprod. Genet. 36 (6), 1211–1223. doi:10.1007/s10815-019-01463-4
De Cock, L. J., De Koker, S., De Vos, F., Vervaet, C., Remon, J.-P., and De Geest, B. G. (2010). Layer-by-layer Incorporation of Growth Factors in Decellularized Aortic Heart Valve Leaflets. Biomacromolecules 11 (4), 1002–1008. doi:10.1021/bm9014649
De Santis, M. M., Alsafadi, H. N., Tas, S., Bölükbas, D. A., Prithiviraj, S., Da Silva, I. A. N., et al. (2021). Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue. Adv. Mater. 33 (3), e2005476. doi:10.1002/adma.202005476
Deeken, C. R., White, A. K., Bachman, S. L., Ramshaw, B. J., Cleveland, D. S., Loy, T. S., et al. (2011). Method of Preparing a Decellularized Porcine Tendon Using Tributyl Phosphate. J. Biomed. Mater. Res. 96B (2), 199–206. doi:10.1002/jbm.b.31753
Denstedt, J., and Atala, A. (2009). Biomaterials and Tissue Engineering in Urology. Amsterdam, Netherlands: Elsevier.
Deuse, T., Hu, X., Gravina, A., Wang, D., Tediashvili, G., De, C., et al. (2019). Hypoimmunogenic Derivatives of Induced Pluripotent Stem Cells Evade Immune Rejection in Fully Immunocompetent Allogeneic Recipients. Nat. Biotechnol. 37 (3), 252–258. doi:10.1038/s41587-019-0016-3
Dew, L., English, W. R., Chong, C. K., and MacNeil, S. (2016). Investigating Neovascularization in Rat Decellularized Intestine: an In Vitro Platform for Studying Angiogenesis. Tissue Eng. Part. A. 22 (23-24), 1317–1326. doi:10.1089/ten.TEA.2016.0131
Dimitrievska, S., Cai, C., Weyers, A., Balestrini, J. L., Lin, T., Sundaram, S., et al. (2015). Click-coated, Heparinized, Decellularized Vascular Grafts. Acta Biomater. 13, 177–187. doi:10.1016/j.actbio.2014.11.015
Divya, D., Naik, S., Raju, O., Shivani, B., Basappa, N., and Betur, A. (2021). Conceptual Combination of Disinfection in Regenerative Endodontics: Conventional versus Laser-Assisted Disinfection. J. Conserv Dent 24 (3), 252–259. doi:10.4103/jcd.jcd_96_21
Du, P., Hwang, M. P., Noh, Y. K., Subbiah, R., Kim, I. G., Bae, S. E., et al. (2014). Fibroblast-derived Matrix (FDM) as a Novel Vascular Endothelial Growth Factor Delivery Platform. J. Controlled Release 194, 122–129. doi:10.1016/j.jconrel.2014.08.026
Duarte, M. M., Ribeiro, N., Silva, I. V., Dias, J. R., Alves, N. M., and Oliveira, A. L. (2021). Fast Decellularization Process Using Supercritical Carbon Dioxide for Trabecular Bone. J. Supercrit. Fluids 172, 105194. doi:10.1016/j.supflu.2021.105194
Dunne, L. W., Huang, Z., Meng, W., Fan, X., Zhang, N., Zhang, Q., et al. (2014). Human Decellularized Adipose Tissue Scaffold as a Model for Breast Cancer Cell Growth and Drug Treatments. Biomaterials 35 (18), 4940–4949. doi:10.1016/j.biomaterials.2014.03.003
Ebrahimkhani, M. R., and Levin, M. (2021). Synthetic Living Machines: A New Window on Life. Iscience 24, 102505. doi:10.1016/j.isci.2021.102505
Eltom, A., Zhong, G., and Muhammad, A. (2019). Scaffold Techniques and Designs in Tissue Engineering Functions and Purposes: A Review. Adv. Mater. Sci. Eng. 2019, 3429527. doi:10.1155/2019/3429527
Engler, A. J., Sen, S., Sweeney, H. L., and Discher, D. E. (2006). Matrix Elasticity Directs Stem Cell Lineage Specification. Cell 126 (4), 677–689. doi:10.1016/j.cell.2006.06.044
Fernández-Pérez, J., and Ahearne, M. (2019). The Impact of Decellularization Methods on Extracellular Matrix Derived Hydrogels. Sci. Rep. 9 (1), 14933. doi:10.1038/s41598-019-49575-2
Fernández-Pérez, J., and Ahearne, M. (2019). The Impact of Decellularization Methods on Extracellular Matrix Derived Hydrogels. Scientific Rep. 9 (1), 1–12.
Ferng, A. S., Connell, A. M., Marsh, K. M., Qu, N., Medina, A. O., Bajaj, N., et al. (2017). Acellular Porcine Heart Matrices: Whole Organ Decellularization with 3D-Bioscaffold & Vascular Preservation. J. Clin. Transl Res. 3 (2), 260–270.
Ferreira, L. P., Gaspar, V. M., and Mano, J. F. (2020). Decellularized Extracellular Matrix for Bioengineering Physiomimetic 3D In Vitro Tumor Models. Trends Biotechnol. 38 (12), 1397–1414. doi:10.1016/j.tibtech.2020.04.006
Fidalgo, C., Iop, L., Sciro, M., Harder, M., Mavrilas, D., Korossis, S., et al. (2018). A Sterilization Method for Decellularized Xenogeneic Cardiovascular Scaffolds. Acta Biomater. 67, 282–294. doi:10.1016/j.actbio.2017.11.035
Figliuzzi, M., Bonandrini, B., and Remuzzi, A. (2017). Decellularized Kidney Matrix as Functional Material for Whole Organ Tissue Engineering. J. Appl. Biomater. Funct. Mater. 15 (4), e326–e333. doi:10.5301/jabfm.5000393
Fitzpatrick, L. E., and McDevitt, T. C. (2015). Cell-derived Matrices for Tissue Engineering and Regenerative Medicine Applications. Biomater. Sci. 3 (1), 12–24. doi:10.1039/c4bm00246f
Fitzpatrick, J. C., Clark, P. M., and Capaldi, F. M. (2008). Effect of Decellularization Protocol on the Mechanical Behavior of Porcine Descending Aorta. Int. J. Biomater. 2010, 620503. doi:10.1155/2010/620503
Forouzesh, F., Rabbani, M., and Bonakdar, S. (2019). A Comparison between Ultrasonic bath and Direct Sonicator on Osteochondral Tissue Decellularization. J. Med. Signals Sens 9 (4), 227–233. doi:10.4103/jmss.JMSS_64_18
Frantz, C., Stewart, K. M., and Weaver, V. M. (2010). The Extracellular Matrix at a Glance. J. Cel. Sci. 123 (24), 4195–4200. doi:10.1242/jcs.023820
Fu, Y., Fan, X., Chen, P., Shao, C., and Lu, W. (2010). Reconstruction of a Tissue-Engineered Cornea with Porcine Corneal Acellular Matrix as the Scaffold. Cells Tissues Organs 191 (3), 193–202. doi:10.1159/000235680
Fu, R. H., Wang, Y. C., Liu, S. P., Shih, T. R., Lin, H. L., Chen, Y. M., et al. (2014). Decellularization and Recellularization Technologies in Tissue Engineering. Cel Transpl. 23 (4-5), 621–630. doi:10.3727/096368914X678382
Funamoto, S., Nam, K., Kimura, T., Murakoshi, A., Hashimoto, Y., Niwaya, K., et al. (2010). The Use of High-Hydrostatic Pressure Treatment to Decellularize Blood Vessels. Biomaterials 31 (13), 3590–3595. doi:10.1016/j.biomaterials.2010.01.073
Gaetani, R., Aude, S., DeMaddalena, L. L., Strassle, H., Dzieciatkowska, M., Wortham, M., et al. (2018). Evaluation of Different Decellularization Protocols on the Generation of Pancreas-Derived Hydrogels. Tissue Eng. C: Methods 24 (12), 697–708. doi:10.1089/ten.tec.2018.0180
Ganjibakhsh, M., Mehraein, F., Koruji, M., Aflatoonian, R., and Farzaneh, P. (2019). Three-dimensional Decellularized Amnion Membrane Scaffold as a Novel Tool for Cancer Research; Cell Behavior, Drug Resistance and Cancer Stem Cell Content. Mater. Sci. Eng. C 100, 330–340. doi:10.1016/j.msec.2019.02.090
García-Gareta, E., Abduldaiem, Y., Sawadkar, P., Kyriakidis, C., Lali, F., and Greco, K. V. (2020). Decellularised Scaffolds: Just a Framework? Current Knowledge and Future Directions. J. Tissue Eng. 11, 2041731420942903. doi:10.1177/2041731420942903
Garreta, E., Oria, R., Tarantino, C., Pla-Roca, M., Prado, P., Fernández-Avilés, F., et al. (2017). Tissue Engineering by Decellularization and 3D Bioprinting. Mater. Today 20 (4), 166–178. doi:10.1016/j.mattod.2016.12.005
Ghodsizad, A., Bordel, V., Wiedensohler, H., Elbanayosy, A., Koerner, M. M., Gonzalez Berjon, J. M., et al. (2014). Magnetically Guided Recellularization of Decellularized Stented Porcine Pericardium-Derived Aortic Valve for TAVI. ASAIO J. 60 (5), 582–586. doi:10.1097/mat.0000000000000110
Ghorbani, F., Moradi, L., Shadmehr, M. B., Bonakdar, S., Droodinia, A., and Safshekan, F. (2017). In-vivo Characterization of a 3D Hybrid Scaffold Based on PCL/decellularized Aorta for Tracheal Tissue Engineering. Mater. Sci. Eng. C 81, 74–83. doi:10.1016/j.msec.2017.04.150
Gilbert, T. W., Sellaro, T. L., and Badylak, S. F. (2006). Decellularization of Tissues and Organs. Biomaterials 27 (19), 3675–3683. doi:10.1016/j.biomaterials.2006.02.014
Gilpin, A., and Yang, Y. (2017). Decellularization Strategies for Regenerative Medicine: from Processing Techniques to Applications. Biomed. Res. Int. 2017, 9831534. doi:10.1155/2017/9831534
Gilpin, S. E., Guyette, J. P., Gonzalez, G., Ren, X., Asara, J. M., Mathisen, D. J., et al. (2014). Perfusion Decellularization of Human and Porcine Lungs: Bringing the Matrix to Clinical Scale. J. Heart Lung Transplant. 33 (3), 298–308. doi:10.1016/j.healun.2013.10.030
Gilpin, S. E., Ren, X., Okamoto, T., Guyette, J. P., Mou, H., Rajagopal, J., et al. (2014). Enhanced Lung Epithelial Specification of Human Induced Pluripotent Stem Cells on Decellularized Lung Matrix. Ann. Thorac. Surg. 98 (5), 1721–1729. doi:10.1016/j.athoracsur.2014.05.080
Giraldo-Gomez, D. M., García-López, S. J., Tamay-de-Dios, L., Sánchez-Sánchez, R., Villalba-Caloca, J., Sotres-Vega, A., et al. (2019). Fast Cyclical-Decellularized Trachea as a Natural 3D Scaffold for Organ Engineering. Mater. Sci. Eng. C. 105, 110142. doi:10.1016/j.msec.2019.110142
Giuffrida, P., Curti, M., Al-Akkad, W., Biel, C., Crowley, C., Frenguelli, L., et al. (2019). Decellularized Human Gut as a Natural 3D Platform for Research in Intestinal Fibrosis. Inflamm. Bowel Dis. 25 (11), 1740–1750. doi:10.1093/ibd/izz115
Goktas, S., Matuska, A. M., Pierre, N., Gibson, T. M., Dmytryk, J. J., and McFetridge, P. S. (2014). Decellularization Method Influences Early Remodeling of an Allogenic Tissue Scaffold. J. Biomed. Mater. Res. 102 (1), 8–16. The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. doi:10.1002/jbm.a.34692
Gong, W., Lei, D., Li, S., Huang, P., Qi, Q., Sun, Y., et al. (2016). Hybrid Small-Diameter Vascular Grafts: Anti-expansion Effect of Electrospun Poly ε-caprolactone on Heparin-Coated Decellularized Matrices. Biomaterials 76, 359–370. doi:10.1016/j.biomaterials.2015.10.066
Gosztyla, C., Ladd, M. R., Werts, A., Fulton, W., Johnson, B., Sodhi, C., et al. (2020). A Comparison of Sterilization Techniques for Production of Decellularized Intestine in Mice. Tissue Eng. Part C: Methods 26 (2), 67–79. doi:10.1089/ten.tec.2019.0219
Grauss, R., Hazekamp, M., Oppenhuizen, F., Vanmunsteren, C., Gittenbergerdegroot, A., and DeRuiter, M. (2005). Histological Evaluation of Decellularised Porcine Aortic Valves: Matrix Changes Due to Different Decellularisation Methods. Eur. J. Cardio-Thoracic Surg. 27 (4), 566–571. doi:10.1016/j.ejcts.2004.12.052
Gubareva, E. A., Sjöqvist, S., Gilevich, I. V., Sotnichenko, A. S., Kuevda, E. V., Lim, M. L., et al. (2016). Orthotopic Transplantation of a Tissue Engineered Diaphragm in Rats. Biomaterials 77, 320–335. doi:10.1016/j.biomaterials.2015.11.020
Gupta, S. K., Mishra, N. C., and Dhasmana, A. (2017). Decellularization Methods for Scaffold Fabrication. Decellularized Scaffolds and Organogenesis. Methods Mol. Biol. 1577, 1–10. doi:10.1007/7651_2017_34
Gupta, S. K., Mishra, N. C., and Dhasmana, A. (2018). “Decellularization Methods for Scaffold Fabrication,” in Decellularized Scaffolds and Organogenesis: Methods and Protocols. Editor K. Turksen (New York, NY: Springer New York), 1–10.
Han, T. T. Y., Toutounji, S., Amsden, B. G., and Flynn, L. E. (2015). Adipose-derived Stromal Cells Mediate In Vivo Adipogenesis, Angiogenesis and Inflammation in Decellularized Adipose Tissue Bioscaffolds. Biomaterials 72, 125–137. doi:10.1016/j.biomaterials.2015.08.053
Heath, D. E. (2019). A Review of Decellularized Extracellular Matrix Biomaterials for Regenerative Engineering Applications. Regen. Eng. Transl. Med. 5 (2), 155–166. doi:10.1007/s40883-018-0080-0
Hellström, M., Moreno-Moya, J. M., Bandstein, S., Bom, E., Akouri, R. R., Miyazaki, K., et al. (2016). Bioengineered Uterine Tissue Supports Pregnancy in a Rat Model. Fertil. Steril 106 (2), 487–e1. doi:10.1016/j.fertnstert.2016.03.048
Hennessy, R. S., Jana, S., Tefft, B. J., Helder, M. R., Young, M. D., Hennessy, R. R., et al. (2017). Supercritical Carbon Dioxide-Based Sterilization of Decellularized Heart Valves. JACC: Basic Translational Sci. 2 (1), 71–84. doi:10.1016/j.jacbts.2016.08.009
Hou, N., Cui, P., Luo, J., Ma, R., and Zhu, L. (2011). Tissue-engineered Larynx Using Perfusion-Decellularized Technique and Mesenchymal Stem Cells in a Rabbit Model. Acta oto-laryngologica 131 (6), 645–652. doi:10.3109/00016489.2010.547517
Huang, H., Zhang, J., and Chu, H. (2007). Feasibility of Constructing Tissue-Engineered Corpus Cavernous Urethra with Rabbit Bone Marrow Mesenchymal Stem Cells. Zhongguo Zuzhi Gongcheng Yanjiu Yu Linchuang Kangfu 11, 2665–2668. doi:10.1016/j.biomaterials.2014.03.003
Huang, M., Li, N., Wu, Z., Wan, P., Liang, X., Zhang, W., et al. (2011). Using Acellular Porcine Limbal Stroma for Rabbit Limbal Stem Cell Microenvironment Reconstruction. Biomaterials 32 (31), 7812–7821. doi:10.1016/j.biomaterials.2011.07.012
Hudson, T. W., Liu, S. Y., and Schmidt, C. E. (2004). Engineering an Improved Acellular Nerve Graft via Optimized Chemical Processing. Tissue Eng. 10 (9-10), 1346–1358. doi:10.1089/ten.2004.10.1641
Huh, M. I., Lee, K. P., Kim, J., Yi, S., Park, B. U., and Kim, H. K. (2018). Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering. J. Ophthalmol. 2018, 2590536. doi:10.1155/2018/2590536
Hussein, K. H., Park, K.-M., Kang, K.-S., and Woo, H.-M. (2016). Biocompatibility Evaluation of Tissue-Engineered Decellularized Scaffolds for Biomedical Application. Mater. Sci. Eng. C 67, 766–778. doi:10.1016/j.msec.2016.05.068
Hussein, K. H., Saleh, T., Ahmed, E., Kwak, H.-H., Park, K.-M., Yang, S.-R., et al. (2018). Biocompatibility and Hemocompatibility of Efficiently Decellularized Whole Porcine Kidney for Tissue Engineering. J. Biomed. Mater. Res. 106 (7), 2034–2047. doi:10.1002/jbm.a.36407
Isidan, A., Liu, S., Li, P., Lashmet, M., Smith, L. J., Hara, H., et al. (2019). Decellularization Methods for Developing Porcine Corneal Xenografts and Future Perspectives. Xenotransplantation 26 (6), e12564. doi:10.1111/xen.12564
Islam, M. M., Sharifi, R., Mamodaly, S., Islam, R., Nahra, D., Abusamra, D. B., et al. (2019). Effects of Gamma Radiation Sterilization on the Structural and Biological Properties of Decellularized Corneal Xenografts. Acta Biomater. 96, 330–344. doi:10.1016/j.actbio.2019.07.002
Jamur, M. C., and Oliver, C. (2010). Cell Fixatives for Immunostaining. Methods Mol. Biol. 588, 55–61. doi:10.1007/978-1-59745-324-0_8
Ji, S., and Guvendiren, M. (2017). Recent Advances in Bioink Design for 3D Bioprinting of Tissues and Organs. Front. Bioeng. Biotechnol. 5, 23. doi:10.3389/fbioe.2017.00023
Johnson, C. M., Guo, D., Ryals, S., Postma, G. N., and Weinberger, P. M. (2017). The Feasibility of Gamma Radiation Sterilization for Decellularized Tracheal Grafts. The Laryngoscope 127 (8), E258–E264. doi:10.1002/lary.26367
Joszko, K., Gzik-Zroska, B., Kawlewska, E., Klama-Baryła, A., Suchoń, S., Burkacki, M., et al. (2019). Evaluation of the Impact of Decellularization and Sterilization on Tensile Strength Transgenic Porcinedermal Dressings. Acta Bioeng. Biomech. 21 (3), 87–97.
Kai, D., Jin, G., Prabhakaran, M. P., and Ramakrishna, S. (2013). Electrospun Synthetic and Natural Nanofibers for Regenerative Medicine and Stem Cells. Biotechnol. J. 8 (1), 59–72. doi:10.1002/biot.201200249
Kajbafzadeh, A.-M., Javan-Farazmand, N., Monajemzadeh, M., and Baghayee, A. (2013). Determining the Optimal Decellularization and Sterilization Protocol for Preparing a Tissue Scaffold of a Human-Sized Liver Tissue. Tissue Eng. Part C: Methods 19 (8), 642–651. doi:10.1089/ten.tec.2012.0334
Kajbafzadeh, A.-M., Khorramirouz, R., Nabavizadeh, B., Ladi Seyedian, S.-S., Akbarzadeh, A., Heidari, R., et al. (2019). Whole Organ Sheep Kidney Tissue Engineering and In Vivo Transplantation: Effects of Perfusion-Based Decellularization on Vascular Integrity. Mater. Sci. Eng. C 98, 392–400. doi:10.1016/j.msec.2019.01.018
Kajbafzadeh, A. M., Fendereski, K., Khorramirouz, R., Daryabari, S. S., Masoomi, A., Moosavi, S., et al. (2020). In Vivo application of Decellularized Rat colon and Evaluation of the Engineered Scaffolds Following 9 Months of Follow‐up. Cell Biol Int 44 (11), 2253–2262. doi:10.1002/cbin.11433
Kao, C.-Y., Nguyen, H.-Q. -D., and Weng, Y.-C. (2020). Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method. Polymers 12 (12), 3007. doi:10.3390/polym12123007
Kawecki, M., Łabuś, W., Klama-Baryla, A., Kitala, D., Kraut, M., Glik, J., et al. (2018). A Review of Decellurization Methods Caused by an Urgent Need for Quality Control of Cell-free Extracellular Matrix' Scaffolds and Their Role in Regenerative Medicine. J. Biomed. Mater. Res. 106 (2), 909–923. doi:10.1002/jbm.b.33865
Keane, T. J., Swinehart, I. T., and Badylak, S. F. (2015). Methods of Tissue Decellularization Used for Preparation of Biologic Scaffolds and In Vivo Relevance. Methods 84, 25–34. doi:10.1016/j.ymeth.2015.03.005
Kim, J. K., Koh, Y.-D., Kim, J. O., and Seo, D. H. (2016). Development of a Decellularization Method to Produce Nerve Allografts Using Less Invasive Detergents and Hyper/hypotonic Solutions. J. Plast. Reconstr. Aesthet. Surg. 69 (12), 1690–1696. doi:10.1016/j.bjps.2016.08.016
Kim, J., Koo, B.-K., and Knoblich, J. A. (2020). Human Organoids: Model Systems for Human Biology and Medicine. Nat. Rev. Mol. Cel Biol 21 (10), 571–584. doi:10.1038/s41580-020-0259-3
Komai, Y., and Ushiki, T. (1991). The Three-Dimensional Organization of Collagen Fibrils in the Human Cornea and Sclera. Invest. Ophthalmol. Vis. Sci. 32 (8), 2244–2258.
Kuevda, E. V., Gubareva, E. A., Sotnichenko, A. S., Gumenyuk, I. S., Gilevich, I. V., Polyakov, I. S., et al. (2016). Experience of Perfusion Recellularization of Biological Lung Scaffold in Rats. Rjtao 18 (1), 38–44. doi:10.15825/1995-1191-2016-1-38-44
Kuevda, E. V., Gubareva, E. A., Nakokhov, R. Z., Verevkin, A. A., and Puzanov, D. P. (2019). Obtaining and Characterization of Cells from Native Lung and Diaphragm Rat Tissues for Recellularization of Tissue Engineered Constructs. Cell Tiss. Biol. 13 (2), 136–143. doi:10.1134/s1990519x19020056
Kumar Kuna, V., Xu, B., and Sumitran-Holgersson, S. (2018). Decellularization and Recellularization Methodology for Human Saphenous Veins. J. visualized experiments: JoVE. (137), e57803. doi:10.3791/57803
Langer, R., and Vacanti, J. (2016). Advances in Tissue Engineering. J. Pediatr. Surg. 51 (1), 8–12. doi:10.1016/j.jpedsurg.2015.10.022
Lawson, J. H., Glickman, M. H., Ilzecki, M., Jakimowicz, T., Jaroszynski, A., Peden, E. K., et al. (2016). Bioengineered Human Acellular Vessels for Dialysis Access in Patients with End-Stage Renal Disease: Two Phase 2 Single-Arm Trials. The Lancet 387 (10032), 2026–2034. doi:10.1016/s0140-6736(16)00557-2
Lee, J., Hong, J., Kim, W., and Kim, G. H. (2020). Bone-derived dECM/alginate Bioink for Fabricating a 3D Cell-Laden Mesh Structure for Bone Tissue Engineering. Carbohydr. Polym. 250, 116914. doi:10.1016/j.carbpol.2020.116914
Liao, J., Joyce, E. M., and Sacks, M. S. (2008). Effects of Decellularization on the Mechanical and Structural Properties of the Porcine Aortic Valve Leaflet. Biomaterials 29 (8), 1065–1074. doi:10.1016/j.biomaterials.2007.11.007
Liao, J., Xu, B., Zhang, R., Fan, Y., Xie, H., and Li, X. (2020). Applications of Decellularized Materials in Tissue Engineering: Advantages, Drawbacks and Current Improvements, and Future Perspectives. J. Mater. Chem. B 8 (44), 10023–10049. doi:10.1039/d0tb01534b
Lichtenberg, A., Tudorache, I., Cebotari, S., Ringes-Lichtenberg, S., Sturz, G., Hoeffler, K., et al. (2006). In Vitro re-endothelialization of Detergent Decellularized Heart Valves under Simulated Physiological Dynamic Conditions. Biomaterials 27 (23), 4221–4229. doi:10.1016/j.biomaterials.2006.03.047
Lin, H. J., Wang, T. J., Li, T. W., Chang, Y. Y., Sheu, M. T., Huang, Y. Y., et al. (2019). Development of Decellularized Cornea by Organic Acid Treatment for Corneal Regeneration. Tissue Eng. Part. A. 25 (7-8), 652–662. doi:10.1089/ten.TEA.2018.0162
Liu, G., Wang, B., Li, S., Jin, Q., and Dai, Y. (2019). Human Breast Cancer Decellularized Scaffolds Promote Epithelial‐to‐mesenchymal Transitions and Stemness of Breast Cancer Cells In Vitro. J. Cell Physiol. 234 (6), 9447–9456. doi:10.1002/jcp.27630
Liu, K., He, Y., Yao, Y., Zhang, Y., Cai, Z., Ru, J., et al. (2021). Methoxy Polyethylene Glycol Modification Promotes Adipogenesis by Inducing the Production of Regulatory T Cells in Xenogeneic Acellular Adipose Matrix. Mater. Today Bio 12, 100161. doi:10.1016/j.mtbio.2021.100161
Liu, X., Yu, K., Cheng, S., Ren, T., Maitusong, M., Liu, F., et al. (2021). Ulvan Mediated VE Cadherin Antibody and REDV Peptide Co-modification to Improve Endothelialization Potential of Bioprosthetic Heart Valves. Mater. Sci. Eng. C 128, 112337. doi:10.1016/j.msec.2021.112337
Lopes Guimarães, L., Aparecida De Brito, A., Gonçalves Santos, T., Daronco Cereta, A., De Oliveira, L. V., Ligeiro De Oliveira, A. P., et al. (2020). Low-level Laser Boosts Extracellular Matrix Cues and Enhances Acellular Lung Scaffold Recellularization. Eur. Respir. J. 56 (Suppl. 64), 561. doi:10.1183/13993003.congress-2020.561
Lopresti, S. T., and Brown, B. N. (2015). “Host Response to Naturally Derived Biomaterials,” in Host Response to Biomaterials. Editor F. Badylak (Cambridge, Massachusetts, United States: Academic Press), 53–79. doi:10.1016/b978-0-12-800196-7.00004-9
Lumpkins, S. B., Pierre, N., and McFetridge, P. S. (2008). A Mechanical Evaluation of Three Decellularization Methods in the Design of a Xenogeneic Scaffold for Tissue Engineering the Temporomandibular Joint Disc. Acta Biomater. 4 (4), 808–816. doi:10.1016/j.actbio.2008.01.016
Márquez, S. P., Martínez, V. S., Ambrose, W. M., Wang, J., Gantxegui, N. G., Schein, O., et al. (2009). Decellularization of Bovine Corneas for Tissue Engineering Applications. Acta Biomater. 5 (6), 1839–1847. doi:10.1016/j.actbio.2009.02.011
Martin, I., Wendt, D., and Heberer, M. (2004). The Role of Bioreactors in Tissue Engineering. Trends. Biotechnol. 22 (2), 80–86. doi:10.1016/j.tibtech.2003.12.001
Mattei, G., Magliaro, C., Pirone, A., and Ahluwalia, A. (2017). Decellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold. Artif. organs 41 (12), E347–E355. doi:10.1111/aor.12925
Matuska, A. M., and McFetridge, P. S. (2018). Laser Micro‐ablation of Fibrocartilage Tissue: Effects of Tissue Processing on Porosity Modification and Mechanics. J. Biomed. Mater. Res. 106 (5), 1858–1868. doi:10.1002/jbm.b.33997
McCrary, M. W., Bousalis, D., Mobini, S., Song, Y. H., and Schmidt, C. E. (2020). Decellularized Tissues as Platforms for In Vitro Modeling of Healthy and Diseased Tissues. Acta Biomater. 111, 1–19. doi:10.1016/j.actbio.2020.05.031
McCrary, M. W., Vaughn, N. E., Hlavac, N., Song, Y. H., Wachs, R. A., and Schmidt, C. E. (2020). Novel Sodium Deoxycholate-Based Chemical Decellularization Method for Peripheral Nerve. Tissue Eng. Part C: Methods 26 (1), 23–36. doi:10.1089/ten.tec.2019.0135
Mendez, J. J., Ghaedi, M., Steinbacher, D., and Niklason, L. E. (2014). Epithelial Cell Differentiation of Human Mesenchymal Stromal Cells in Decellularized Lung Scaffolds. Tissue Eng. Part. A. 20 (11-12), 1735–1746. doi:10.1089/ten.TEA.2013.0647
Mendibil, U., Ruiz-Hernandez, R., Retegi-Carrion, S., Garcia-Urquia, N., Olalde-Graells, B., and Abarrategi, A. (2020). Tissue-specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds. Ijms 21 (15), 5447. doi:10.3390/ijms21155447
Mimler, T., Nebert, C., Eichmair, E., Winter, B., Aschacher, T., Stelzmueller, M.-E., et al. (2019). Extracellular Matrix in Ascending Aortic Aneurysms and Dissections - what We Learn from Decellularization and Scanning Electron Microscopy. PLoS ONE 14 (3), e0213794. doi:10.1371/journal.pone.0213794
Montoya, C. V., and McFetridge, P. S. (2009). Preparation of Ex Vivo-Based Biomaterials Using Convective Flow Decellularization. Tissue Eng. Part C: Methods 15 (2), 191–200. doi:10.1089/ten.tec.2008.0372
Moradi, L., Mohammadi Jobania, B., Jafarnezhad-Ansariha, F., Ghorbani, F., Esmaeil-Pour, R., Majidi Zolbina, M., et al. (2020). Evaluation of Different Sterilization Methods for Decellularized Kidney Tissue. Tissue and Cell 66, 101396. doi:10.1016/j.tice.2020.101396
Moradi, L., Mohammadi Jobania, B., Jafarnezhad-Ansariha, F., Ghorbani, F., Esmaeil-Pour, R., Majidi Zolbina, M., et al. (2020). Evaluation of Different Sterilization Methods for Decellularized Kidney Tissue. Tissue and Cell 66, 101396. doi:10.1016/j.tice.2020.101396
Morris, A. H., Lee, H., Xing, H., Stamer, D. K., Tan, M., and Kyriakides, T. R. (2018). Tunable Hydrogels Derived from Genetically Engineered Extracellular Matrix Accelerate Diabetic Wound Healing. ACS Appl. Mater. Inter. 10 (49), 41892–41901. doi:10.1021/acsami.8b08920
Moser, P. T., and Ott, H. C. (2014). Recellularization of Organs. Curr. Opin. Organ Transplant. 19 (6), 603–609. doi:10.1097/mot.0000000000000131
Mullarky, M. B., Norris, C. W., and Goldberg, I. D. (1985). The Efficacy of the Co2 Laser in the Sterilization of Skin Seeded with Bacteria. The Laryngoscope 95 (2), 186–187. doi:10.1288/00005537-198502000-00011
Naik, A., Griffin, M., Szarko, M., and Butler, P. E. (2020). Optimizing the Decellularization Process of an Upper Limb Skeletal Muscle; Implications for Muscle Tissue Engineering. Artif. Organs 44 (2), 178–183. doi:10.1111/aor.13575
Nakayama, K. H., Lee, C. C. I., Batchelder, C. A., and Tarantal, A. F. (2013). Tissue Specificity of Decellularized Rhesus Monkey Kidney and Lung Scaffolds. PLoS ONE 8 (5), e64134. doi:10.1371/journal.pone.0064134
Nam, J., Huang, Y., Agarwal, S., and Lannutti, J. (2007). Improved Cellular Infiltration in Electrospun Fiber via Engineered Porosity. Tissue Eng. 13 (9), 2249–2257. doi:10.1089/ten.2006.0306
Nammour, S., and Majerus, P. (1991). Sterilization Potential of the CO2 Laser. Acta Stomatol Belg. 88 (4), 183–186.
Oliveira, A. C., Garzón, I., Ionescu, A. M., Carriel, V., Cardona, J. d. l. C., González-Andrades, M., et al. (2013). Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering. PLoS ONE 8 (6), e66538. doi:10.1371/journal.pone.0066538
O'Neill, J. D., Anfang, R., Anandappa, A., Costa, J., Javidfar, J., Wobma, H. M., et al. (2013). Decellularization of Human and Porcine Lung Tissues for Pulmonary Tissue Engineering. Ann. Thorac. Surg. 96 (3), 1046–1056. doi:10.1016/j.athoracsur.2013.04.022
Ozasa, Y., Amadio, P. C., Thoreson, A. R., An, K.-N., and Zhao, C. (2013). The Effect of Surface Modification on Gliding Ability of Decellularized Flexor Tendon in a Canine Model In Vitro. J. Hand Surg. 38 (9), 1698–1704. doi:10.1016/j.jhsa.2013.05.023
Papadimitropoulos, A., Scotti, C., Bourgine, P., Scherberich, A., and Martin, I. (2015). Engineered Decellularized Matrices to Instruct Bone Regeneration Processes. Bone 70, 66–72. doi:10.1016/j.bone.2014.09.007
Partington, L., Mordan, N. J., Mason, C., Knowles, J. C., Kim, H.-W., Lowdell, M. W., et al. (2013). Biochemical Changes Caused by Decellularization May Compromise Mechanical Integrity of Tracheal Scaffolds. Acta Biomater. 9 (2), 5251–5261. doi:10.1016/j.actbio.2012.10.004
Peng, X., Kristi, N., Gafur, A., Wang, J., Wang, G., and Ye, Z. (2019). Micromechanical Property Analyses of Decellularized Vessels by Atomic Force Microscopy. J. Phys. D: Appl. Phys. 52 (42), 425401. doi:10.1088/1361-6463/ab33ce
Philips, C., Campos, F., Roosens, A., Sánchez-Quevedo, M. d. C., Declercq, H., and Carriel, V. (2018). Qualitative and Quantitative Evaluation of a Novel Detergent-Based Method for Decellularization of Peripheral Nerves. Ann. Biomed. Eng. 46 (11), 1921–1937. doi:10.1007/s10439-018-2082-y
Philips, C., Campos, F., Roosens, A., Sánchez-Quevedo, M. d. C., Declercq, H., and Carriel, V. (2018). Qualitative and Quantitative Evaluation of a Novel Detergent-Based Method for Decellularization of Peripheral Nerves. Ann. Biomed. Eng. 46 (11), 1921–1937. doi:10.1007/s10439-018-2082-y
Pina, S., Ribeiro, V. P., Marques, C. F., Maia, F. R., Silva, T. H., Reis, R. L., et al. (2019). Scaffolding Strategies for Tissue Engineering and Regenerative Medicine Applications. Materials (Basel) 12 (11), 1824. doi:10.3390/ma12111824
Pinto, M. L., Rios, E., Silva, A. C., Neves, S. C., Caires, H. R., Pinto, A. T., et al. (2017). Decellularized Human Colorectal Cancer Matrices Polarize Macrophages towards an Anti-inflammatory Phenotype Promoting Cancer Cell Invasion via CCL18. Biomaterials 124, 211–224. doi:10.1016/j.biomaterials.2017.02.004
Poornejad, N., Schaumann, L. B., Buckmiller, E. M., Momtahan, N., Gassman, J. R., Ma, H. H., et al. (2016). The Impact of Decellularization Agents on Renal Tissue Extracellular Matrix. J. Biomater. Appl. 31 (4), 521–533. doi:10.1177/0885328216656099
Pors, S. E., Ramløse, M., Nikiforov, D., Lundsgaard, K., Cheng, J., Andersen, C. Y., et al. (2019). Initial Steps in Reconstruction of the Human Ovary: Survival of Pre-antral Stage Follicles in a Decellularized Human Ovarian Scaffold. Hum. Reprod. 34 (8), 1523–1535. doi:10.1093/humrep/dez077
Porzionato, A., Stocco, E., Barbon, S., Grandi, F., Macchi, V., and De Caro, R. (2018). Tissue-engineered Grafts from Human Decellularized Extracellular Matrices: a Systematic Review and Future Perspectives. Ijms 19 (12), 4117. doi:10.3390/ijms19124117
Pouliot, R. A., Young, B. M., Link, P. A., Park, H. E., Kahn, A. R., Shankar, K., et al. (2020). Porcine Lung-Derived Extracellular Matrix Hydrogel Properties Are Dependent on Pepsin Digestion Time. Tissue Eng. Part C: Methods 26 (6), 332–346. doi:10.1089/ten.tec.2020.0042
Prasertsung, I., Kanokpanont, S., Bunaprasert, T., Thanakit, V., and Damrongsakkul, S. (2008). Development of Acellular Dermis from Porcine Skin Using Periodic Pressurized Technique. J. Biomed. Mater. Res. 85B (1), 210–219. The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. doi:10.1002/jbm.b.30938
Pulver, S. A., Leybovich, B., Artyuhov, I., Maleev, Y., and Peregudov, A. (2014). Production of Organ Extracellular Matrix Using a Freeze-Thaw Cycle Employing Extracellular Cryoprotectants. Cryo Lett. 35 (5), 400–406.
Qiu, S., Rao, Z., He, F., Wang, T., Xu, Y., Du, Z., et al. (2020). Decellularized Nerve Matrix Hydrogel and Glial‐derived Neurotrophic Factor Modifications Assisted Nerve Repair with Decellularized Nerve Matrix Scaffolds. J. Tissue Eng. Regen. Med. 14 (7), 931–943. doi:10.1002/term.3050
Rabbani, M., Zakian, N., and Alimoradi, N. (2021). Contribution of Physical Methods in Decellularization of Animal Tissues. J. Med. Signals Sens 11 (1), 1–11. doi:10.4103/jmss.jmss_2_20
Rahman, S., Griffin, M., Naik, A., Szarko, M., and Butler, P. E. (2018). Optimising the Decellularization of Human Elastic Cartilage with Trypsin for Future Use in Ear Reconstruction. Sci. Rep. 8 (1), 1–11. doi:10.1038/s41598-018-20592-x
Reing, J. E., Brown, B. N., Daly, K. A., Freund, J. M., Gilbert, T. W., Hsiong, S. X., et al. (2010). The Effects of Processing Methods upon Mechanical and Biologic Properties of Porcine Dermal Extracellular Matrix Scaffolds. Biomaterials 31 (33), 8626–8633. doi:10.1016/j.biomaterials.2010.07.083
Reyna, W. E., Pichika, R., Ludvig, D., and Perreault, E. J. (2020). Efficiency of Skeletal Muscle Decellularization Methods and Their Effects on the Extracellular Matrix. J. Biomech. 110, 109961. doi:10.1016/j.jbiomech.2020.109961
Rieder, E., Kasimir, M.-T., Silberhumer, G., Seebacher, G., Wolner, E., Simon, P., et al. (2004). Decellularization Protocols of Porcine Heart Valves Differ Importantly in Efficiency of Cell Removal and Susceptibility of the Matrix to Recellularization with Human Vascular Cells. J. Thorac. Cardiovasc. Surg. 127 (2), 399–405. doi:10.1016/j.jtcvs.2003.06.017
Robertson, M. J., Dries-Devlin, J. L., Kren, S. M., Burchfield, J. S., and Taylor, D. A. (2014). Optimizing Recellularization of Whole Decellularized Heart Extracellular Matrix. PloS one 9 (2), e90406. doi:10.1371/journal.pone.0090406
Roth, S. P., Glauche, S. M., Plenge, A., Erbe, I., Heller, S., and Burk, J. (2017). Automated Freeze-Thaw Cycles for Decellularization of Tendon Tissue - a Pilot Study. BMC Biotechnol. 17 (1), 13–10. doi:10.1186/s12896-017-0329-6
Sabetkish, S., Kajbafzadeh, A.-M., Sabetkish, N., Khorramirouz, R., Akbarzadeh, A., Seyedian, S. L., et al. (2015). Whole-organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Liver Scaffolds. J. Biomed. Mater. Res. 103 (4), 1498–1508. doi:10.1002/jbm.a.35291
Sabetkish, S., Kajbafzadeh, A.-M., and Sabetkish, N. (2021). Recellularization of Testicular Feminization Testis in C57bl6 as a Natural Bioreactor for Creation of Cellularized Seminiferous Tubules: an Experimental Study. Cell Tissue Bank 22 (2), 287–295. doi:10.1007/s10561-020-09877-6
Saldin, L. T., Cramer, M. C., Velankar, S. S., White, L. J., and Badylak, S. F. (2017). Extracellular Matrix Hydrogels from Decellularized Tissues: Structure and Function. Acta Biomater. 49, 1–15. doi:10.1016/j.actbio.2016.11.068
Saleh, T., Ahmed, E., Yu, L., Kwak, H.-H., Kang, B.-J., Park, K.-M., et al. (2019). Characterization of Silver Nanoparticle-Modified Decellularized Rat Esophagus for Esophageal Tissue Engineering: Structural Properties and Biocompatibility. J. Biosci. Bioeng. 128 (5), 613–621. doi:10.1016/j.jbiosc.2019.04.017
Sant, S., Wang, D., Abidi, M., Walker, G., and Ferrell, N. (2021). Mechanical Characterization of Native and Sugar-Modified Decellularized Kidneys. J. Mech. Behav. Biomed. Mater. 114, 104220. doi:10.1016/j.jmbbm.2020.104220
Scarrit, M. E., Pashos, N. C., and Bunnell, B. A. (2015). A Review of Cellularization Strategies for Tissue Engineering of Whole Organs. Front. Bioeng. Biotechnol. 3, 43. doi:10.3389/fbioe.2015.00043
Schenke-Layland, K., Rofail, F., Heydarkhan, S., Gluck, J. M., Ingle, N. P., Angelis, E., et al. (2009). The Use of Three-Dimensional Nanostructures to Instruct Cells to Produce Extracellular Matrix for Regenerative Medicine Strategies. Biomaterials 30 (27), 4665–4675. doi:10.1016/j.biomaterials.2009.05.033
Schneider, K. H., Rohringer, S., Kapeller, B., Grasl, C., Kiss, H., Heber, S., et al. (2020). Riboflavin-mediated Photooxidation to Improve the Characteristics of Decellularized Human Arterial Small Diameter Vascular Grafts. Acta Biomater. 116, 246–258. doi:10.1016/j.actbio.2020.08.037
Selden, C., and Fuller, B. (2018). Role of Bioreactor Technology in Tissue Engineering for Clinical Use and Therapeutic Target Design. Bioengineering 5 (2), 32. doi:10.3390/bioengineering5020032
Shafiee, A., and Atala, A. (2017). Tissue Engineering: toward a new era of Medicine. Annu. Rev. Med. 68, 29–40. doi:10.1146/annurev-med-102715-092331
Shen, Q., Goderie, S. K., Jin, L., Karanth, N., Sun, Y., Abramova, N., et al. (2004). Endothelial Cells Stimulate Self-Renewal and Expand Neurogenesis of Neural Stem Cells. science 304 (5675), 1338–1340. doi:10.1126/science.1095505
Sheridan, W. S., Duffy, G. P., and Murphy, B. P. (2012). Mechanical Characterization of a Customized Decellularized Scaffold for Vascular Tissue Engineering. J. Mech. Behav. Biomed. Mater. 8, 58–70. doi:10.1016/j.jmbbm.2011.12.003
Shevach, M., Fleischer, S., Shapira, A., and Dvir, T. (2014). Gold Nanoparticle-Decellularized Matrix Hybrids for Cardiac Tissue Engineering. Nano Lett. 14 (10), 5792–5796. doi:10.1021/nl502673m
Simmons, A. D., Williams, C., Degoix, A., and Sikavitsas, V. I. (2017). Sensing Metabolites for the Monitoring of Tissue Engineered Construct Cellularity in Perfusion Bioreactors. Biosens. Bioelectron. 90, 443–449. doi:10.1016/j.bios.2016.09.094
Simsa, R., Padma, A. M., Heher, P., Hellström, M., Teuschl, A., Jenndahl, L., et al. (2018). Systematic In Vitro Comparison of Decellularization Protocols for Blood Vessels. PLoS One 13 (12), e0209269. doi:10.1371/journal.pone.0209269
Simsa, R., Vila, X. M., Salzer, E., Teuschl, A., Jenndahl, L., Bergh, N., et al. (2019). Effect of Fluid Dynamics on Decellularization Efficacy and Mechanical Properties of Blood Vessels. PLoS ONE 14 (8), e0220743. doi:10.1371/journal.pone.0220743
Smith, M. J., Dempsey, S. G., Veale, R. W., Duston-Fursman, C. G., Rayner, C. A. F., Javanapong, C., et al. (2022). Further Structural Characterization of Ovine Forestomach Matrix and Multi-Layered Extracellular Matrix Composites for Soft Tissue Repair. J. Biomater. Appl. 36 (6), 996–1010. doi:10.1177/08853282211045770
Soltani Khaboushan, A., Shakibaei, M., Kajbafzadeh, A-M., and Majidi Zolbin, M. (2021). Prenatal Neural Tube Anomalies: A Decade of Intrauterine Stem Cell Transplantation Using Advanced Tissue Engineering Methods. Stem Cel Rev. Rep. 18, 1–16. doi:10.1007/s12015-021-10150-w
Song, J. J., Guyette, J. P., Gilpin, S. E., Gonzalez, G., Vacanti, J. P., and Ott, H. C. (2013). Regeneration and Experimental Orthotopic Transplantation of a Bioengineered Kidney. Nat. Med. 19 (5), 646–651. doi:10.1038/nm.3154
Song, Y. H., Maynes, M. A., Hlavac, N., Visosevic, D., Daramola, K. O., Porvasnik, S. L., et al. (2021). Development of Novel Apoptosis-Assisted Lung Tissue Decellularization Methods. Biomater. Sci. 9 (9), 3485–3498. doi:10.1039/d1bm00032b
Starnecker, F., König, F., Hagl, C., and Thierfelder, N. (2018). Tissue-engineering Acellular Scaffolds-The Significant Influence of Physical and Procedural Decellularization Factors. J. Biomed. Mater. Res. 106 (1), 153–162. doi:10.1002/jbm.b.33816
Stenn, K. S., Link, R., Moellmann, G., Madri, J., and Kuklinska, E. (1989). Dispase, a Neutral Protease from Bacillus Polymyxa, Is a Powerful Fibronectinase and Type IV Collagenase. J. Invest. Dermatol. 93 (2), 287–290. doi:10.1111/1523-1747.ep12277593
Su, J., Satchell, S. C., Shah, R. N., and Wertheim, J. A. (2018). Kidney Decellularized Extracellular Matrix Hydrogels: Rheological Characterization and Human Glomerular Endothelial Cell Response to Encapsulation. J. Biomed. Mater. Res. 106 (9), 2448–2462. doi:10.1002/jbm.a.36439
Tao, M., Ao, T., Mao, X., Yan, X., Javed, R., Hou, W., et al. (2021). Sterilization and Disinfection Methods for Decellularized Matrix Materials: Review, Consideration and Proposal. Bioactive Mater. 6 (9), 2927–2945. doi:10.1016/j.bioactmat.2021.02.010
Tenreiro, M. F., Almeida, H. V., Calmeiro, T., Fortunato, E., Ferreira, L., Alves, P. M., et al. (2021). Interindividual Heterogeneity Affects the Outcome of Human Cardiac Tissue Decellularization. Sci. Rep. 11 (1), 1–11. doi:10.1038/s41598-021-00226-5
Topuz, B., Günal, G., Guler, S., and Aydin, H. M. (2020). Use of Supercritical CO2 in Soft Tissue Decellularization. Methods Cel. Biol. 157, 49–79. doi:10.1016/bs.mcb.2019.10.012
Tsuchiya, T., Balestrini, J. L., Mendez, J., Calle, E. A., Zhao, L., and Niklason, L. E. (2014). Influence of pH on Extracellular Matrix Preservation during Lung Decellularization. Tissue Eng. Part C: Methods 20 (12), 1028–1036. doi:10.1089/ten.tec.2013.0492
Tsuchiya, T., Mendez, J., Calle, E. A., Hatachi, G., Doi, R., Zhao, L., et al. (2016). Ventilation-based Decellularization System of the Lung. BioResearch open access 5 (1), 118–126. doi:10.1089/biores.2016.0012
Tsuchiya, T., Mitsutake, N., Nishimura, S., Matsuu-Matsuyama, M., Nakazawa, Y., Ogi, T., et al. (2017). Transplantation of Bioengineered Rat Lungs Recellularized with Endothelial and Adipose-Derived Stromal Cells. Sci. Rep. 7 (1), 1–5. doi:10.1038/s41598-017-09115-2
Uday Chandrika, K., Tripathi, R., Kameshwari, Y., Rangaraj, N., Mahesh Kumar, J., and Singh, S. (2021). Refunctionalization of Decellularized Organ Scaffold of Pancreas by Recellularization: Whole Organ Regeneration into Functional Pancreas. Tissue Eng. Regen. Med. 18 (1), 99–112. doi:10.1007/s13770-020-00296-y
Uygun, B. E., Soto-Gutierrez, A., Yagi, H., Izamis, M.-L., Guzzardi, M. A., Shulman, C., et al. (2010). Organ Reengineering through Development of a Transplantable Recellularized Liver Graft Using Decellularized Liver Matrix. Nat. Med. 16 (7), 814–820. doi:10.1038/nm.2170
Vacanti, J. P., and Langer, R. (1999). Tissue Engineering: the Design and Fabrication of Living Replacement Devices for Surgical Reconstruction and Transplantation. The lancet 354, S32–S34. doi:10.1016/s0140-6736(99)90247-7
Versteegden, L. R. M., de Jonge, P. K. J. D., IntHout, J., van Kuppevelt, T. H., Oosterwijk, E., Feitz, W. F. J., et al. (2017). Tissue Engineering of the Urethra: a Systematic Review and Meta-Analysis of Preclinical and Clinical Studies. Eur. Urol. 72 (4), 594–606. doi:10.1016/j.eururo.2017.03.026
Walawalkar, S., and Almelkar, S. (2019). Fabrication of Aortic Bioprosthesis by Decellularization, Fibrin Glue Coating and Re-endothelization: a Cell Scaffold Approach. Prog. Biomater. 8 (3), 197–210. doi:10.1007/s40204-019-00122-2
Wang, L., Zhao, M., Li, S., Erasquin, U. J., Wang, H., Ren, L., et al. (2014). "Click" Immobilization of a VEGF-Mimetic Peptide on Decellularized Endothelial Extracellular Matrix to Enhance Angiogenesis. ACS Appl. Mater. Inter. 6 (11), 8401–8406. doi:10.1021/am501309d
Wang, Z., Mithieux, S. M., and Weiss, A. S. (2019). Fabrication Techniques for Vascular and Vascularized Tissue Engineering. Adv. Healthc. Mater. 8 (19), 1900742. doi:10.1002/adhm.201900742
Wang, Z., Han, L., Sun, T., Ma, J., Sun, S., Ma, L., et al. (2020). Extracellular Matrix Derived from Allogenic Decellularized Bone Marrow Mesenchymal Stem Cell Sheets for the Reconstruction of Osteochondral Defects in Rabbits. Acta Biomater. 118, 54–68. doi:10.1016/j.actbio.2020.10.022
Wasyłeczko, M., Sikorska, W., and Chwojnowski, A. (2020). Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering. Membranes (Basel) 10 (11), 348. doi:10.3390/membranes10110348
Watanabe, N., Mizuno, M., Matsuda, J., Nakamura, N., Otabe, K., Katano, H., et al. (2019). Comparison of High‐Hydrostatic‐Pressure Decellularized versus Freeze‐Thawed Porcine Menisci. J. Orthop. Res. 37 (11), 2466–2475. doi:10.1002/jor.24350
Wiles, K., Fishman, J. M., De Coppi, P., and Birchall, M. A. (2016). The Host Immune Response to Tissue-Engineered Organs: Current Problems and Future Directions. Tissue Eng. B: Rev. 22 (3), 208–219. doi:10.1089/ten.teb.2015.0376
Wilson, S. L., Sidney, L. E., Dunphy, S. E., Dua, H. S., and Hopkinson, A. (2016). Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation? Curr. Eye Res. 41 (6), 769–782. doi:10.3109/02713683.2015.1062114
Wishart, A. L., Conner, S. J., Guarin, J. R., Fatherree, J. P., Peng, Y., McGinn, R. A., et al. (2020). Decellularized Extracellular Matrix Scaffolds Identify Full-Length Collagen VI as a Driver of Breast Cancer Cell Invasion in Obesity and Metastasis. Sci. Adv. 6 (43), eabc3175. doi:10.1126/sciadv.abc3175
Wolf, M. T., Daly, K. A., Brennan-Pierce, E. P., Johnson, S. A., Carruthers, C. A., D'Amore, A., et al. (2012). A Hydrogel Derived from Decellularized Dermal Extracellular Matrix. Biomaterials 33 (29), 7028–7038. doi:10.1016/j.biomaterials.2012.06.051
Wolf, M. T., Dearth, C. L., Ranallo, C. A., LoPresti, S. T., Carey, L. E., Daly, K. A., et al. (2014). Macrophage Polarization in Response to ECM Coated Polypropylene Mesh. Biomaterials 35 (25), 6838–6849. doi:10.1016/j.biomaterials.2014.04.115
Wollmann, L., Suss, P., Mendonça, J., Luzia, C., Schittini, A., Rosa, G. W. X. D., et al. (2019). Characterization of Decellularized Human Pericardium for Tissue Engineering and Regenerative Medicine Applications. Arq Bras Cardiol. 113, 11–17. doi:10.5935/abc.20190094
Woods, T., and Gratzer, P. F. (2005). Effectiveness of Three Extraction Techniques in the Development of a Decellularized Bone-Anterior Cruciate Ligament-Bone Graft. Biomaterials 26 (35), 7339–7349. doi:10.1016/j.biomaterials.2005.05.066
Wu, Z., Zhou, Y., Li, N., Huang, M., Duan, H., Ge, J., et al. (2009). The Use of Phospholipase A2 to Prepare Acellular Porcine Corneal Stroma as a Tissue Engineering Scaffold. Biomaterials 30 (21), 3513–3522. doi:10.1016/j.biomaterials.2009.03.003
Wu, Q., Li, Y., Wang, Y., Li, L., Jiang, X., Tang, J., et al. (2016). The Effect of Heparinized Decellularized Scaffolds on Angiogenic Capability. J. Biomed. Mater. Res. 104 (12), 3021–3030. doi:10.1002/jbm.a.35843
Xing, Q., Yates, K., Tahtinen, M., Shearier, E., Qian, Z., and Zhao, F. (2015). Decellularization of Fibroblast Cell Sheets for Natural Extracellular Matrix Scaffold Preparation. Tissue Eng. Part C: Methods 21 (1), 77–87. doi:10.1089/ten.tec.2013.0666
Yang, H., Atala, A., and Yoo, J. J. (2020). Kidney Regeneration Approaches for Translation. World J. Urol. 38 (9), 2075–2079. doi:10.1007/s00345-019-02999-x
Young, D. A., Ibrahim, D. O., Hu, D., and Christman, K. L. (2011). Injectable Hydrogel Scaffold from Decellularized Human Lipoaspirate. Acta Biomater. 7 (3), 1040–1049. doi:10.1016/j.actbio.2010.09.035
Yusof, F., Sha'ban, M., and Azhim, A. (2019). Development of Decellularized Meniscus Using Closed Sonication Treatment System: Potential Scaffolds for Orthopedics Tissue Engineering Applications. Ijn Vol. 14, 5491–5502. doi:10.2147/ijn.s207270
Zhang, X., Chen, X., Hong, H., Hu, R., Liu, J., and Liu, C. (2021). Decellularized Extracellular Matrix Scaffolds: Recent Trends and Emerging Strategies in Tissue Engineering. Bioactive Mater. 10, 15–31. doi:10.1016/j.bioactmat.2021.09.014
Zhao, Y., Zhang, S., Zhou, J., Wang, J., Zhen, M., Liu, Y., et al. (2010). The Development of a Tissue-Engineered Artery Using Decellularized Scaffold and Autologous Ovine Mesenchymal Stem Cells. Biomaterials 31 (2), 296–307. doi:10.1016/j.biomaterials.2009.09.049
Zheng, X.-F., Lu, S.-B., Zhang, W.-G., Liu, S.-Y., Huang, J.-X., and Guo, Q.-Y. (2011). Mesenchymal Stem Cells on a Decellularized Cartilage Matrix for Cartilage Tissue Engineering. Biotechnol. Bioproc. E 16 (3), 593–602. doi:10.1007/s12257-010-0348-9
Zhou, J., Fritze, O., Schleicher, M., Wendel, H.-P., Schenke-Layland, K., Harasztosi, C., et al. (2010). Impact of Heart Valve Decellularization on 3-D Ultrastructure, Immunogenicity and Thrombogenicity. Biomaterials 31 (9), 2549–2554. doi:10.1016/j.biomaterials.2009.11.088
Zvarova, B., Uhl, F. E., Uriarte, J. J., Borg, Z. D., Coffey, A. L., Bonenfant, N. R., et al. (2016). Residual Detergent Detection Method for Nondestructive Cytocompatibility Evaluation of Decellularized Whole Lung Scaffolds. Tissue Eng. Part C: Methods 22 (5), 418–428. doi:10.1089/ten.tec.2015.0439
Glossary
ECM extracellular matrix
dECM decellularized extracellular matrix
3D three-dimensional
GAG glycosaminoglycans
SDS sodium dodecyl sulfate
SD sodium deoxycholate
SB sulfobetaine
TnBP tri (n-butyl) phosphate
CHAPS 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
DNase deoxyribonuclease
EGTA ethylene glycol tetraacetic acid
EDTA ethylenediaminetetraacetic acid
PAA peracetic acid
RNase ribonuclease
UV ultraviolet
MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide
dsDNA double-stranded DNA
H&E hematoxylin and eosin
DAPI 4’,6-diamidino-2-phenylindole
SEM scanning electron microscope
TEM transmission electron microscopy
IHC immunohistochemistry
MSCs mesenchymal stem cells
AFM atomic force microscopy
VEGF vascular endothelial growth factor
POC poly(octamethylene citrate)
SIS small intestinal submucosa
ADSCs adipose-derived stem cells
DAMP damage associated molecular pattern
TIPS thermal-induced phase separation
Au gold
NP nanoparticle
Ag silver
PLGA poly lactic-co-glycolic acid
bFGF basic fibroblast growth factor
iPSCs induced pluripotent stem cells
ESCs embryonic stem cells
HSCs hematopoietic stem cells
BM-MSCs bone marrow-derived mesenchymal stem cells
Keywords: extracellular matrix, recellularization, regenerative medicine, decellularizalion, tissue engineering
Citation: Neishabouri A, Soltani Khaboushan A, Daghigh F, Kajbafzadeh A-M and Majidi Zolbin M (2022) Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods. Front. Bioeng. Biotechnol. 10:805299. doi: 10.3389/fbioe.2022.805299
Received: 30 October 2021; Accepted: 04 April 2022;
Published: 25 April 2022.
Edited by:
Kamal Hany Hussein, Assiut University, EgyptReviewed by:
Renata Kelly Da Palma, Universidad Francisco de Vitoria, SpainChiara Magliaro, University of Pisa, Italy
Xiaojun Yu, Stevens Institute of Technology, United States
Copyright © 2022 Neishabouri, Soltani Khaboushan, Daghigh, Kajbafzadeh and Majidi Zolbin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Masoumeh Majidi Zolbin, Masoumeh.majidizolbin@gmail.com; Abdol-Mohammad Kajbafzadeh, kajbafzd@tums.ac.ir
†These authors share first authorship