Injectable and Temperature-Sensitive Titanium Carbide-Loaded Hydrogel System for Photothermal Therapy of Breast Cancer

Recently, organic–inorganic hybrid materials have gained much attention as effective photothermal agents for cancer treatment. In this study, Pluronic F127 hydrogel-coated titanium carbide (Ti₃C₂) nanoparticles were utilized as an injectable photothermal agent. The advantages of these nanoparticles are their green synthesis and excellent photothermal efficiency. In this system, lasers were mainly used to irradiate Ti₃C₂ nanoparticles to produce a constant high temperature, which damaged cancer cells. The nanoparticles were found to be stable during storage at low temperatures for at least 2 weeks. The Ti₃C₂ nanoparticles exhibited a shuttle-shaped structure, and the hydrogels presented a loosely meshed structure. In addition, Ti₃C₂ nanoparticles did not affect the reversible temperature sensitivity of the gel, and the hydrogel did not affect the photothermal properties of Ti₃C₂ nanoparticles. The in vitro and in vivo results show that this hydrogel system can effectively inhibit tumor growth upon exposure to near-infrared irradiation with excellent biocompatibility and biosafety. The photothermal agent-embedded hydrogel is a promising photothermal therapeutic strategy for cancer treatment by enhancing the retention in vivo and elevating the local temperature in tumors.

Introduction

Photothermal therapy (PTT) has been widely used in cancer therapy because of its excellent antitumor effects (Senapati et al., 2018; Zhi et al., 2020). In PTT, a near-infrared (NIR: 700–1,100 nm) laser is used to irradiate the target area where a PTT agent is present. This produces a constant high temperature (40°C–50°C), which either induces the death of local cancer cells or increases their sensitivity to other therapies (Chen et al., 2017; Yu et al., 2017; Zhao et al., 2018; Lu et al., 2020). PTT has shown significant therapeutic effects in various antitumor studies (Jiang et al., 2018; Zhu et al., 2018; Chen et al., 2019a). Many photothermal agents (PTAs) have been developed to convert the energy of NIR lasers into heat for cancer research, including organic dye molecules (Sahu et al., 2016), precious metal materials (Xu et al., 2019), carbon-based materials (Liu et al., 2019a; Rafieerad et al., 2019), and other inorganic materials (Popescu et al., 2011). PTA is often administered intravenously (Popescu et al., 2011; Jiang et al., 2018; Zhang et al., 2018; Zhi et al., 2020). However, there are some concerns regarding the toxicity of intravenously administered PTAs. Most PTAs contain heavy metals. In addition, the inherent instability of organic agents limits their therapeutic effects. For example, indocyanine green often undergoes light bleaching and is rapidly eliminated after intravenous administration (Sahu et al., 2016; Chen et al., 2017; Yu et al., 2017).

Therefore, nanomaterials with good photothermal stability have been designed to overcome the limitations of traditional PTAs by using an NIR laser to increase the temperature (Chen et al., 2019b; He et al., 2019; Xu et al., 2019; Zhao et al., 2019; He et al., 2020; Wang et al., 2020). As an outstanding nanomaterial, titanium carbide (Ti₃C₂) shows good performance in the anticancer field of PTT. For example, Ti₃C₂ can absorb large amounts of light and shows high light-thermal conversion efficiency and high biocompatibility at NIR wavelengths. Photothermal nanomaterials can be actively concentrated in tumor tissues by modifying the nanoparticle surface to avoid systemic toxicity (He et al., 2019; Zhao et al., 2019). The treatment conditions (laser power, exposure positions, and exposure times) can be artificially controlled to minimize damage to the surrounding healthy tissue. In addition, photothermal nanomaterials can be actively targeted to tumor sites using ultrasonic or magnetic guidance (Senapati et al., 2018; Chen et al., 2019b). However, nanomaterials with targeted functions not only require costly materials and complex operations but also have a short half-life in the body (Xu et al., 2019; He et al., 2020; Liu et al., 2020; Lu et al., 2020). Thus, a simple and effective local slow-release administration system for PTT is urgently needed.

Currently, commonly used intratumoral or peritumoral sustained-release delivery systems include microneedles (Yu et al., 2020) and hydrogels (Fu et al., 2019; Geng et al., 2020), which can prolong drug release into the targeted tissue. For example, in situ gels contain a drug loaded in a specific polymer carrier. In situ gels adopt a sol form in vitro and quickly form a gel after administration in vivo, prolonging drug retention in the tumor (Liu et al., 2017; Geng et al., 2020). The gel formation mechanism can be illustrated by Pluronic F127 (F127), a three-segment copolymer that consists of polyoxymethylene and polyoxypropylene. At low temperatures, F127 exists as a single molecule. As the temperature increases, the hydrophobic fragments of polyoxypropylene in the F127 molecule dehydrate to form spherical gel beams consisting of an inner core of hydrophobic polyoxypropylene and external swollen polyoxyethylene. Subsequently, the stacked bundles further wind to form a gel (Chen et al., 2019a; Russo and Villa, 2019; Yu et al., 2021). As a mild-sensitized pharmaceutical accessory, F127 limits the random dispersion of nanoparticles within tissues to avoid damage to normal tissues (Fu et al., 2019).

To effectively treat breast cancer and reduce the side effects of traditional PTA, we designed an injectable and temperature-sensitive F127 hydrogel mixed with Ti₃C₂ nanoparticles. The Ti₃C₂ nanoparticles and temperature-sensitive F127 hydrogel jointly construct the Ti₃C₂-Gel system, which is administered by local injection at low temperatures and forms a gel at 37°C in the body. The results of in vivo and in vitro experiments showed that the system had an excellent antitumor effect. In general, the combination of Ti₃C₂ nanoparticles and temperature-sensitive hydrogels is a promising antitumor local administration system with the potential for use in clinical cancer treatments.

Materials and Methods

Materials

Ti₃C₂ MXene solution was purchased from Beike 2D Materials Co., Ltd. (Beijing, China). Pluronic F127 was purchased from Sigma (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum were purchased from Gibco (Grand Island, NY, USA). The Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Laboratories (Kumamoto, Japan). The hematoxylin–eosin staining kit (H&E) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) kit were purchased from Solarbio (Beijing, China). All other reagents were analytically pure and used without further purification. Deionized water was used in this study.

Cell and Mice

Mouse breast cancer cells (4T1 cells) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in DMEM containing 1% penicillin–streptomycin and 10% fetal bovine serum at 37°C under a 5% CO₂ atmosphere. Female BALB/c mice (6–8 weeks of age) were obtained from Charles River Laboratories (Wilmington, MA, USA) for animal experiments. All animal experiments were approved by the Animal Care and Use Committees at the First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology.

Preparation and Characterization of Ti₃C₂-Gel

The Ti₃C₂-Gel system was fabricated using a simple mixture. Ti₃C₂ nanoparticles were obtained through ultrasonic treatment of Ti₃C₂ MXene solution. The Ti₃C₂ nanoparticles and thermosensitive Pluronic F127 were mixed to prepare the PTA-embedded hydrogel system (Figure 1). The F127 powder was dissolved in water to form hydrogels with different concentrations (40%, 35%, 30%, 25%, 20%, 19%, and 18%). To obtain different final concentrations of Ti₃C₂ nanoparticles embedded in F127 hydrogels (Ti₃C₂: 400, 200, 100, 50, and 25 μg/ml; F127 hydrogels: 20%), a solution of Ti₃C₂ nanoparticles was added to the 20% F127 hydrogels. These hydrogels (including Gel and Ti₃C₂-Gel) were stored at 4°C until use. After dilution by 50-fold with pure water, the size and zeta potential of these samples (Ti₃C₂ and Ti₃C₂-Gel) were measured by dynamic light scattering (PSS ZPW 388, Nicomp, Orlando, FL, USA). The appearance of these samples was recorded using transmission electron microscopy (JEOL174 1200EX, Tokyo, Japan).

FIGURE 1

FIGURE 1. Schematic illustration of the fabrication of the injectable hydrogel system with excellent photo–heat transition capacity. This system was fabricated using a one-step synthesis method. Ti₃C₂ nanoparticles and thermosensitive Pluronic F127 were mixed to prepare the photothermal agent-embedded hydrogel system. The system can form in situ gel in tumor tissue through sol–gel transition and prolong the retention of Ti₃C₂ nanoparticles. Using near-infrared laser irradiation, repeated treatments can be achieved with a single injection.

To determine the phase change temperature of the hydrogels, different concentrations of blank F127 gel (40%, 35%, 30%, 25%, 20%, 19%, 18%) and Ti₃C₂-Gel (Ti₃C₂: 50 μg/ml) were added to glass bottles and placed at 4°C to maintain the liquid state to remove bubbles from the samples. During this assay, an agitator was placed in the bottles, which were placed in a water bath under a tunable temperature and magnetic stirring. The heating rate was 0.5°C/min, and the rotation rate of the magnetic agitator was 300 rpm. The temperature was recorded as the gel formation temperature when the agitator was completely stopped. Each sample was measured three times.

In addition, the rheumatic behaviors of blank F127-Gel (20%) and Ti₃C₂-Gel (Ti₃C₂: 50 μg/ml; 20% F127 Gel) were observed using a rheometer (MCR92, Anton Paar, Graz, Austria). These samples were placed on the plate of the rheometer, and silicone oil was added to the outer edge of the samples to prevent moisture evaporation.

To study the stability of the Ti₃C₂ nanoparticles and hydrogel sample, Ti₃C₂ nanoparticles (50 μg/ml) and Ti₃C₂-Gel (Ti₃C₂: 50 μg/ml; 20% F127 Gel) were stored at 4°C. The samples were diluted with pure water before and after storage. Changes in the size of the Ti₃C₂ nanoparticles and Ti₃C₂-Gel were measured using a particle sizing system.

Assessment of Photothermal Properties

To evaluate the photothermal conversion capability of Ti₃C₂ nanoparticles aqueous solution and Ti₃C₂-Gel, 1-ml samples were separately added to different centrifuge tubes and then exposed to an 808-nm NIR laser (1 W/cm²). A thermal imaging system (FOTRIC, Allen, TX, USA) was used to record the changes in temperature of the samples.

To further evaluate the stability of photothermal conversion, 1 ml Ti₃C₂ nanoparticle solution (Ti₃C₂: 50 μg/ml) and Ti₃C₂-Gel (Ti₃C₂: 50 μg/ml; 20% F127 Gel) were separately placed into different tubes and then exposed to the NIR laser (1 W/cm²). After the samples had naturally cooled to room temperature (20°C–30°C), they were repeatedly exposed to laser light. We used thermal imaging cameras to record the temperature increase in the samples, which was used to plot a curve of photothermal stability.

In vitro Cytotoxicity

The cytotoxicity of Ti₃C₂ was evaluated using the standard CCK-8 assay. 4T1 cells were seeded at a density of 5 × 10³ cells/well in 96-well plates (37°C, 5% CO₂). After 12 h of incubation, various concentrations of Ti₃C₂ (400, 200, 100, 50, and 25 μg/ml) were dispersed into fresh DMEM and inoculated into the wells. Cells without Ti₃C₂ were used as controls. After incubation for another 24 h, the cells were washed with PBS three times. The absorbance of the cells was measured at 450 nm using a microplate reader. Each group was analyzed in triplicate.

To evaluate the photothermal therapeutic effects of Ti₃C₂ in vitro on tumor cells, 4T1 cells were seeded into 96-well plates (5 × 10³ cells/well) and incubated overnight at 37°C in a 5% CO₂ atmosphere. Next, Ti₃C₂ nanoparticles were added to the wells at final concentrations of 25, 50, 100, 200, and 400 μg/ml. The cells were exposed to an 808-nm NIR laser (1 W/cm²) for 1 min, with untreated 4T1 cells used as a control. The cells were incubated for a further 24 h. The Ti₃C₂ nanoparticles were removed and washed with PBS. Cell viability was measured at 450 nm following the instructions for CCK-8.

In vivo Antitumor Performance

To prepare a 4T1 tumor-bearing mouse model, 1 × 10⁶ 4T1 cells were administered subcutaneously into the right armpit of BALB/c mice (female, 6–8 weeks old). When the tumor volume reached approximately 100 mm³, 4T1 tumor-bearing mice were used for in vivo therapy experiments. The mice were randomly divided into nine groups (n = 5/group): mock (untreated), PBS, Pluronic F127 Gel, Ti₃C₂, Ti₃C₂-Gel, PBS + NIR, Gel + NIR, Ti₃C₂ +NIR, and Ti₃C₂-Gel + NIR (Ti₃C₂: 50 μg/ml; Pluronic F127 Gel: 20%). At day 1, 30 µl of different preparations was administered into the tumor in mice. After the mice were anesthetized, the tumors were exposed to an 808-nm NIR laser at 1 W/cm² for 2 min on days 1, 3, and 5. The length and width of the tumors were measured every other day. Moreover, the body weights of mice were recorded to map the curve of weight–time changes. The tumor volume was calculated according to the following formula: volume = length × (width²)/2. All mice were sacrificed on day 15, and the tumors were collected to evaluate the therapeutic effect of PTT in vivo.

In vivo Safety Evaluation

Blood was collected from anesthetized mice, and the blood samples were placed at room temperature for 2 h and centrifuged at 1,000 × g for 20 min to isolate the serum. Blood biochemistry indicators were detected using a BS-180 automatic biochemical analyzer (Mindray, Shenzhen, China). The tumors were collected for H&E and TUNEL staining to evaluate the toxicity of the treatment.

Statistical Analysis

Quantitative data are expressed as the mean ± standard deviation. Statistical analyses were performed using GraphPad Prism version 8.0 software (GraphPad, Inc, La Jolla, CA, USA). Student’s t-test and one-way analysis of variance were used to test the significance of differences. Statistical significance was set at p < 0.05 (*p < 0.05, **p < 0.01).

Results and Discussion

Characterization of Ti₃C₂ and Ti₃C₂-Gel

As shown in Figure 2A, the Ti₃C₂ MXene solution existed as a two-dimensional flaky substance. After ultrasonic destruction, the flaky structure disintegrated into uniform shuttle-shaped nanoparticles with a size of nearly 50–100 nm, as revealed by transmission electron microscopy (Figure 2B). The particle size of the Ti₃C₂-Gel was approximately 100–200 nm, which was larger than that of the Ti₃C₂ nanoparticles (Figure 2C). The results in Figure 2C show that the F127 hydrogels were coated on the surface of the Ti₃C₂ nanoparticles. Scanning electron microscopy images showed that the blank F127 hydrogels had a loose and porous structure along with a three-dimensional network (Figure 2D). The main elemental compositions of the Ti₃C₂-Gel were Ti, C, and O, suggesting the formation of composites (Figure 2E). Therefore, F127 hydrogels have the potential to easily accommodate large amounts of nanoparticles. In addition, the particle sizes of Ti₃C₂ and Ti₃C₂-Gel were measured using dynamic light scattering, which corroborated the scanning electron microscopy results (Figure 2F). All zeta potential values of the Ti3C₂ nanoparticles, F127 hydrogels, and Ti₃C₂-Gel were positive. To assess the stability of the PTA, the Ti₃C₂ nanoparticles and Ti₃C₂-Gel were stored at 4°C for 2 weeks and examined for changes in particle size (Figure 2H). The results showed that hydrogels are excellent drug reservoirs, and the samples had a stable particle size, indicating that they are suitable for long-term storage.

FIGURE 2

FIGURE 2. Preparation and characterization of Ti₃C₂ nanoparticles and Ti₃C₂-Gel. Transmission electron microscopy image of (A) Ti₃C₂ MXene solution (scale bar: 200 nm), (B) Ti₃C₂ nanoparticles (scale bar: 100 nm), and (C) Ti₃C₂-Gel (scale bar: 200 nm). (D) Scanning electron microscopy image of Pluronic F-127 gel (scale bar: 1 mm). (E) Energy-dispersive X-ray image of Ti₃C₂-Gel (scale bar: 50 nm). (F) Particle sizes of Ti₃C₂ nanoparticles and Ti₃C₂-Gel. (G) Zeta potentials of the different preparations. (H) Stability of Ti₃C₂ and Ti₃C₂-Gel sizes.

Temperature-Sensitive Characteristics of Ti₃C₂-Gel

Figure 3A shows images of the blank F127 hydrogel and Ti₃C₂-Gel at two different temperatures (4°C and 37°C). Both samples were liquids at low temperatures and presented injectable features. They changed to semisolids at 37°C and did not flow even when inverted, which may prolong the retention time of the Ti₃C₂ nanoparticles in the tissue. By test tube inversion in a water bath at 37°C, the time required for the gels to convert from a sol to a gel was 21 ± 1 s. Doping nanoparticles in the gel did not affect the reversible sol–gel transition of the F127 hydrogel. Observation of the samples showed that the nanoparticles were evenly distributed in the F127 hydrogel matrix. To further explain the temperature-sensitive characteristics of the F127 hydrogels, in vitro experiments were conducted to determine the phase transition temperature and rheological performance of the hydrogels. The phase transition temperature is the temperature at which the hydrogel changes from a sol state to a gel state and is among the most important evaluation indicators for temperature-sensitive hydrogels. The phase transition temperatures of the F127 hydrogels differed at different concentrations. As shown in Figures 3B,C, the phase transition temperatures of F127 hydrogels and Ti₃C₂-Gel increased with decreasing concentrations, and Ti₃C₂ had no obvious effect on the phase transition temperature of F127 hydrogels. When the concentration of F127 hydrogels was 18% and 19%, the phase transition temperatures were 36.3 ± 0.245°C and 29.0 ± 0.294°C, respectively. Although the phase transition temperature at an F127 hydrogel concentration of 18% was closer to the human body temperature, in the experiment, 18% and 19% F127 hydrogels were observed to form a second sol state at temperatures greater than 40°C. When the concentration of F127 hydrogels was less than 20%, the kinetic energy of the colloidal particles increased with increasing temperature and accelerated movement speed. Thus, the gel state was unstable, and a concentration of 20% F127 hydrogel was the best choice. It is beneficial for the blank hydrogel and Ti₃C₂-Gel to pass through the syringe needle in a sol state during injection and then quickly transform into a gel state in the body.

FIGURE 3

FIGURE 3. Assessment of photothermal properties. (A) Morphology of Pluronic F127 gel and Ti₃C₂-gel at 4°C and 37°C. (B,C) Phase transition temperature of Pluronic F127 gel and Ti₃C₂-Gel (concentration of Ti₃C₂ was 50 μg/ml). (D,E) Rheological properties of Pluronic F127 and Ti₃C₂-gel (concentration of Pluronic F127 was 20%, and that of Ti₃C₂ was 50 μg/ml).

Next, the complex rheological performances of the blank hydrogel and Ti₃C₂-Gel were detected using a rheometer to draw temperature–viscosity curves (Figures 3D,E). The viscosity profiles of the blank hydrogel and Ti₃C₂-Gel showed similar viscosity characteristics, with a phase transition temperature of 25.4°C, indicating that the Ti₃C₂ nanoparticles had negligible effects on the temperature-sensitive performance of F127 hydrogels. The viscosity of the gels was positively correlated with increased temperature. In the phase transition temperature range, the viscosities of the two formulations increased significantly. The F127 hydrogel was in a sol state, with a viscosity of less than 100 mPa s, ensuring that the entire system was injectable. When the temperature rose to 27°C, the F127 hydrogel adopted a gel state with a stable viscosity higher than 10,000 mPa s, indicating that a drug reservoir could be formed in the body after local administration.

In vitro Photothermal Performance

To evaluate the photothermal performance of this hydrogel system, Ti₃C₂ nanoparticles and Ti₃C₂-Gel were exposed to an 808-nm laser under different conditions (Figures 4A–D). It has been reported that a power of 1 W/cm² is the most commonly used safe and effective range (Chen et al., 2019b; Fu et al., 2019; Zhi et al., 2020). The images and temperature changes of these samples were recorded using a thermal imaging system. Based on the heating curve and images, Ti₃C₂ and the hydrogel system showed excellent photothermal properties. Under the same irradiation power, the increase in the temperature of samples in the tube was concentration- and time-dependent. In addition, the temperature of the Ti₃C₂ nanoparticle solution (50 μg/ml) reached 40°C, whereas that of the Ti₃C₂-Gel system reached 50°C after exposure to the NIR laser for 2 min. The Ti₃C₂ nanoparticles without encapsulation in the hydrogel were so dispersed that the heat generated was more likely to be lost, whereas the gel system was conducive to the accumulation of Ti₃C₂ nanoparticles. In addition, the blank hydrogel exhibited negligible temperature changes under NIR laser exposure.

FIGURE 4

FIGURE 4. In vitro photothermal performance. (A,B) Temperature diagram of Ti₃C₂ nanoparticles and Ti₃C₂-Gel under 808-nm irradiation (1 W/cm²). (C,D) Heating curve of different concentrations of Ti₃C₂ nanoparticles and Ti₃C₂-Gel under 808-nm irradiation (1 W/cm²). (E,F) Heating curves of Ti₃C₂ nanoparticles and Ti₃C₂-Gel treated with repeated 808-nm irradiation (1 W/cm²). (G) Viabilities of 4T1 cells incubated with different concentrations of Ti₃C₂ nanoparticles in DMEM media for 12 h. (H) Cytotoxicity of Ti₃C₂ nanoparticles against 4T1 cells exposed to the 808-nm NIR laser (1 W/cm²) for 1 min (n = 4/group).

An excellent PTA must produce constant high temperatures to facilitate repeated photothermal treatment (Figures 4E,F). To evaluate photothermal stability, the Ti₃C₂ nanoparticle solution (50 μg/ml) and Ti₃C₂-Gel (Ti₃C₂: 50 μg/ml; Pluronic F127 Gel: 20%) were tested by recording heating curves with irradiation (808 nm, 1 W/cm²). The temperature of both Ti₃C₂ nanoparticles and Ti₃C₂-Gel rapidly reached 45°C upon NIR irradiation. The photothermal performance of the Ti₃C₂ nanoparticles and Ti₃C₂-Gel remained constant over five cycles of repeated laser irradiation, indicating the potential of Ti₃C₂-Gel to act as a durable PTA for cancer treatment. The Ti₃C₂-Gel system may achieve multiple treatment effects following a single injection.

Based on the above results, the toxicity and photothermal therapeutic efficacy of Ti₃C₂ on 4T1 cells were investigated in a CCK-8 assay. Figures 4G,H show the survival rate of 4T1 cells against different treatments for 24 h. As observed in the CCK-8 assay, Ti₃C₂ nanoparticles at different concentrations (25, 50, 100, 200, and 400 μg/ml) had no obvious lethal effects on 4T1 cells. The cell viability rates were all above 90%, suggesting negligible toxicity of the Ti₃C₂ nanoparticles. However, cell death was observed after laser exposure (808 nm, 1 W/cm², 1 min). The survival rate of cells varied with the increase in the concentration of Ti₃C₂ nanoparticles under the same laser output power. As the concentration of Ti₃C₂ nanoparticles increased, the survival rate of the cells decreased. At a concentration of 50 μg/ml, most tumor cells were killed, exhibiting an excellent therapeutic effect. Generally, a high concentration of exogenous nanomaterials increases metabolic pressure on animals. To prepare materials economically and safely, and from the perspective of animal welfare, we chose the lowest effective drug concentration.

In vivo Antitumor Performance

Encouraged by the above results, we further evaluated the photothermal antitumor efficacy of Ti₃C₂-Gel in 4T1 tumor-bearing mouse models. After the tumors reached 100 mm³ in size, the 4T1 tumor-bearing mice were randomly divided into nine groups: mock, PBS, Pluronic F127 Gel, Ti₃C₂ aqueous solution, Ti₃C₂-Gel, PBS + NIR, Pluronic F127 Gel + NIR, Ti₃C₂ aqueous solution + NIR, and Ti₃C₂-Gel + NIR. To determine the advantages of the gel system, we used thermal imaging to record the temperature generated upon laser exposure. NIR treatment was performed on days 1, 3, and 5; at 2 weeks after treatment, all tumor tissues were collected to evaluate antitumor efficacy. Notably, upon treatment with Ti₃C₂+NIR and Ti₃C₂-Gel + NIR, tumor growth was significantly suppressed (Figures 5A,F). The tumor tissues treated with the Ti₃C₂ nanoparticles and Ti₃C₂-Gel were smaller than those of the control groups. Using a thermal camera, we observed that the Ti₃C₂+NIR group reached the desired therapeutic temperature upon irradiation at 808 nm at the beginning of the experiment. However, the nanoparticles did not reach the initial high temperature over time, similar to the observations in the other control groups. In contrast, Ti₃C₂+NIR treatment led to a high temperature during the long treatment period (Figures 5B–E). Interestingly, some tumor tissues faded away at the end of treatment with Ti₃C₂-Gel and NIR radiation. During the treatment, the temperature of the tumor tissues increased to 50°C within 2 min, which can disrupt the cell membranes of cancer cells; the underlying mechanisms resulting from PTT have been reported as follows: 1) inhibition of DNA, RNA, and protein synthesis at high temperatures; 2) changes in cell membrane permeability; 3) induction of immunogenic death of cancer cells due to the release of tumor-specific antigens; and 4) production of blood vessel spasms that result in intravascular thrombosis of tumor tissue (Liu et al., 2019b; Poursalehi et al., 2019; Ye et al., 2019; Shang et al., 2020; Chang et al., 2021; Li et al., 2021).

FIGURE 5

FIGURE 5. In vivo antitumor performance. (A) Digital images of tumors of mice after treatment. (B) Thermal images of mice irradiated with an 808-nm laser. (C–E) Temperature in tumor-bearing mice recorded by a thermal imager under the 808-nm laser. (F) Growth curves of tumors in all mice after treatment. n = 5/group, mean ± SD, **p < 0.01. (G) Average body weight of mice in different groups.

These superior antitumor effects were verified by H&E and TUNEL staining of the tumor sections (Figure 6D). The tumor tissues in the Ti₃C₂-Gel + NIR group showed more obvious histological damage compared to the typical tumor structure in other groups, such as partial tumor destruction and necrotic response, indicating significant apoptosis. The body weights of the mice were measured to evaluate the systemic toxicity of the treatments. No obvious body weight loss was observed in these groups during treatment, indicating the biocompatibility of the Ti₃C₂ and F127 hydrogels (Figure 5G). In addition, the indicators of liver and kidney function in all mice were detected using a biochemical analyzer. The blood biochemical indicators of mice treated with Ti₃C₂-Gel showed no obvious differences from those of other control groups, suggesting healthy liver and kidney functions (Figures 6A–C). Similar to previously reported results (Chen et al., 2019a; Fu et al., 2019; Qin et al., 2019), the F127 hydrogel delayed the release of the loaded drug and avoided accumulated toxicity. The in vivo results reveal the potential of the Ti₃C₂-Gel system to exert photothermal therapeutic effects with high biocompatibility and biosafety.

FIGURE 6

FIGURE 6. In vivo safety evaluation. (A–C) Blood biochemistry analysis (including indicators of liver and kidney function) of mice after various treatments. n = 3/group, mean ± SD. (D) Hematoxylin and eosin staining and TUNEL staining of tumor sections from different treatment groups.

Conclusion

In summary, we constructed an injectable and biodegradable theranostic system based on Ti₃C₂ nanoparticles and Ti₃C₂-Gel for robust PTT. The Ti₃C₂ nanoparticle showed a shuttle structure with a diameter of approximately 50 nm, making it useful as a PTA for PTT. As a thermosensitive hydrogel approved by the FDA, F127 is combined with Ti₃C₂ nanoparticles to form a versatile photothermal gel system through a simple mixture. In addition, in vivo and in vitro experiments showed that the gel system has an excellent therapeutic effect against breast cancer. The Ti₃C₂-Gel can elevate the temperature of tumor tissues to ablate 4T1 cancer cells upon relatively mild laser exposure (40–50°C). The Ti₃C₂-Gel system not only has excellent photo-heat conversion and photothermal stability under repeated irradiation but also can form gels in situ in the body to increase the retention time and reduce the toxic side effects of the nanoparticles. As a result, this injectable Ti₃C₂-Gel system provides a translational paradigm for the photothermal treatment of breast cancer.

Data Availability Statement

The raw data supporting the conclusion of this article will be made available by the authors, without undue reservation.

Ethics Statement

The animal study was reviewed and approved by The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology.

Author Contributions

JY and CZ contributed equally to this work. JY and QM conceived and organized the project. CZ and QM completed the experimental part. CZ was responsible for writing the paper. SG and TP collected and summarized the relevant literature. SG provided fund support and revised this article.

Funding

This work was financially supported by the National Natural Science Foundation of China (81972571).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

Chang, M., Hou, Z., Wang, M., Li, C., and Lin, J. (2021). Recent Advances in Hyperthermia Therapy-Based Synergistic Immunotherapy. Adv. Mater. 33 (4), e2004788. doi:10.1002/adma.202004788

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, J., Ning, C., Zhou, Z., Yu, P., Zhu, Y., Tan, G., et al. (2019). Nanomaterials as Photothermal Therapeutic Agents. Prog. Mater. Sci. 99, 1–26. doi:10.1016/j.pmatsci.2018.07.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, Y., Khan, A. R., Yu, D., Zhai, Y., Ji, J., Shi, Y., et al. (2019). Pluronic F127-Functionalized Molybdenum Oxide Nanosheets with pH-dependent Degradability for Chemo-Photothermal Cancer Therapy. J. Colloid Interf. Sci. 553, 567–580. doi:10.1016/j.jcis.2019.06.066

CrossRef Full Text | Google Scholar

Chen, Y., Li, H., Deng, Y., Sun, H., Ke, X., and Ci, T. (2017). Near-infrared Light Triggered Drug Delivery System for Higher Efficacy of Combined Chemo-Photothermal Treatment. Acta Biomater. 51, 374–392. doi:10.1016/j.actbio.2016.12.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Fu, J., Wu, B., Wei, M., Huang, Y., Zhou, Y., Zhang, Q., et al. (2019). Prussian Blue Nanosphere-Embedded In Situ Hydrogel for Photothermal Therapy by Peritumoral Administration. Acta Pharmaceutica Sinica B 9 (3), 604–614. doi:10.1016/j.apsb.2018.12.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Geng, S., Zhao, H., Zhan, G., Zhao, Y., and Yang, X. (2020). Injectable In Situ Forming Hydrogels of Thermosensitive Polypyrrole Nanoplatforms for Precisely Synergistic Photothermo-Chemotherapy. ACS Appl. Mater. Inter. 12 (7), 7995–8005. doi:10.1021/acsami.9b22654

PubMed Abstract | CrossRef Full Text | Google Scholar

He, R.-X., Wang, Q., Li, B., Jia, J., Lu, W.-J., and Shuang, S.-M. (2020). Construction of Protein-Mediated Copper Sulfide Bonded Mesoporous Silica Nanoparticles Vector for Chemo-Photothermal Synergistic Therapy of Cancer. Chin. J. Anal. Chem. 48 (2), 197–205. doi:10.1016/s1872-2040(19)61216-8

CrossRef Full Text | Google Scholar

He, W., Jiang, Y., Li, Q., Zhang, D., Li, Z., and Luan, Y. (2019). A Versatile Strategy to Create an Active Tumor-Targeted Chemo-Photothermal Therapy Nanoplatform: A Case of an IR-780 Derivative Co-assembled with Camptothecin Prodrug. Acta Biomater. 84, 356–366. doi:10.1016/j.actbio.2018.11.049

PubMed Abstract | CrossRef Full Text | Google Scholar

Jiang, K., Zhang, L., Hu, Q., Yue, D., Zhang, J., Zhang, X., et al. (2018). Indocyanine green-encapsulated Nanoscale Metal-Organic Frameworks for Highly Effective Chemo-Photothermal Combination Cancer Therapy. Mater. Today Nano 2, 50–57. doi:10.1016/j.mtnano.2018.09.001

CrossRef Full Text | Google Scholar

Li, J., Zhang, W., Ji, W., Wang, J., Wang, N., Wu, W., et al. (2021). Near Infrared Photothermal Conversion Materials: Mechanism, Preparation, and Photothermal Cancer Therapy Applications. J. Mater. Chem. B 9 (38), 7909–7926. doi:10.1039/d1tb01310f

CrossRef Full Text | Google Scholar

Liu, B., Sun, J., Zhu, J., Li, B., Ma, C., Gu, X., et al. (2020). Injectable and NIR-Responsive DNA-Inorganic Hybrid Hydrogels with Outstanding Photothermal Therapy. Adv. Mater. 32 (39), e2004460. doi:10.1002/adma.202004460

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, M., Song, X., Wen, Y., Zhu, J.-L., and Li, J. (2017). Injectable Thermoresponsive Hydrogel Formed by Alginate-G-Poly(N-Isopropylacrylamide) that Releases Doxorubicin-Encapsulated Micelles as a Smart Drug Delivery System. ACS Appl. Mater. Inter. 9 (41), 35673–35682. doi:10.1021/acsami.7b12849

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, W., Zhang, X., Zhou, L., Shang, L., and Su, Z. (2019). Reduced Graphene Oxide (rGO) Hybridized Hydrogel as a Near-Infrared (NIR)/pH Dual-Responsive Platform for Combined Chemo-Photothermal Therapy. J. Colloid Interf. Sci. 536, 160–170. doi:10.1016/j.jcis.2018.10.050

CrossRef Full Text | Google Scholar

Liu, Y., Bhattarai, P., Dai, Z., and Chen, X. (2019). Photothermal Therapy and Photoacoustic Imaging via Nanotheranostics in Fighting Cancer. Chem. Soc. Rev. 48 (7), 2053–2108. doi:10.1039/c8cs00618k

PubMed Abstract | CrossRef Full Text | Google Scholar

Lu, H., Zhao, Q., Wang, X., Mao, Y., Chen, C., Gao, Y., et al. (2020). Multi-stimuli Responsive Mesoporous Silica-Coated Carbon Nanoparticles for Chemo-Photothermal Therapy of Tumor. Colloids Surf. B: Biointerfaces 190, 110941. doi:10.1016/j.colsurfb.2020.110941

PubMed Abstract | CrossRef Full Text | Google Scholar

Popescu, M.-T., Mourtas, S., Pampalakis, G., Antimisiaris, S. G., and Tsitsilianis, C. (2011). pH-responsive Hydrogel/liposome Soft Nanocomposites for Tuning Drug Release. Biomacromolecules 12 (8), 3023–3030. doi:10.1021/bm2006483

PubMed Abstract | CrossRef Full Text | Google Scholar

Poursalehi, Z., Salehi, R., Samadi, N., Rasta, S. H., Mansoori, B., and Majdi, H. (2019). A Simple Strategy for Chemo-Photothermal Ablation of Breast Cancer Cells by Novel Smart Gold Nanoparticles. Photodiagnosis Photodynamic Ther. 28, 25–37. doi:10.1016/j.pdpdt.2019.08.019

CrossRef Full Text | Google Scholar

Qin, L., Ling, G., Peng, F., Zhang, F., Jiang, S., He, H., et al. (2019). Black Phosphorus Nanosheets and Gemcitabine Encapsulated Thermo-Sensitive Hydrogel for Synergistic Photothermal-Chemotherapy. J. Colloid Interf. Sci. 556, 232–238. doi:10.1016/j.jcis.2019.08.058

CrossRef Full Text | Google Scholar

Rafieerad, A., Yan, W., Sequiera, G. L., Sareen, N., Abu-El-Rub, E., Moudgil, M., et al. (2019). Application of Ti3 C2 MXene Quantum Dots for Immunomodulation and Regenerative Medicine. Adv. Healthc. Mater. 8 (16), e1900569. doi:10.1002/adhm.201900569

PubMed Abstract | CrossRef Full Text | Google Scholar

Russo, E., and Villa, C. (2019). Poloxamer Hydrogels for Biomedical Applications. Pharmaceutics 11 (12). doi:10.3390/pharmaceutics11120671

PubMed Abstract | CrossRef Full Text | Google Scholar

Sahu, A., Lee, J. H., Lee, H. G., Jeong, Y. Y., and Tae, G. (2016). Prussian Blue/serum Albumin/indocyanine green as a Multifunctional Nanotheranostic Agent for Bimodal Imaging Guided Laser Mediated Combinatorial Phototherapy. J. Controlled Release 236, 90–99. doi:10.1016/j.jconrel.2016.06.031

CrossRef Full Text | Google Scholar

Senapati, S., Mahanta, A. K., Kumar, S., and Maiti, P. (2018). Controlled Drug Delivery Vehicles for Cancer Treatment and Their Performance. Sig Transduct Target. Ther. 3, 7. doi:10.1038/s41392-017-0004-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Shang, T., Yu, X., Han, S., and Yang, B. (2020). Nanomedicine-based Tumor Photothermal Therapy Synergized Immunotherapy. Biomater. Sci. 8 (19), 5241–5259. doi:10.1039/d0bm01158d

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, Y., Zhang, H., Xie, J., Liu, Y., Wang, S., and Zhao, Q. (2020). Three Dimensional Mesoporous Carbon Nanospheres as Carriers for Chemo-Photothermal Therapy Compared with Two Dimensional Graphene Oxide Nanosheets. Colloids Surf. A: Physicochemical Eng. Aspects 590. doi:10.1016/j.colsurfa.2020.124498

CrossRef Full Text | Google Scholar

Xu, M., Zhang, K., Liu, Y., Wang, J., Wang, K., and Zhang, Y. (2019). Multifunctional MoS2 Nanosheets with Au NPs Grown In Situ for Synergistic Chemo-Photothermal Therapy. Colloids Surf. B: Biointerfaces 184, 110551. doi:10.1016/j.colsurfb.2019.110551

PubMed Abstract | CrossRef Full Text | Google Scholar

Ye, X., Liang, X., Chen, Q., Miao, Q., Chen, X., Zhang, X., et al. (2019). Surgical Tumor-Derived Personalized Photothermal Vaccine Formulation for Cancer Immunotherapy. ACS Nano 13 (3), 2956–2968. doi:10.1021/acsnano.8b07371

PubMed Abstract | CrossRef Full Text | Google Scholar

Yu, J., Wang, J., Zhang, Y., Chen, G., Mao, W., Ye, Y., et al. (2020). Glucose-responsive Insulin Patch for the Regulation of Blood Glucose in Mice and Minipigs. Nat. Biomed. Eng. 4 (5), 499–506. doi:10.1038/s41551-019-0508-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Yu, Y., Cheng, Y., Tong, J., Zhang, L., Wei, Y., and Tian, M. (2021). Recent Advances in Thermo-Sensitive Hydrogels for Drug Delivery. J. Mater. Chem. B 9 (13), 2979–2992. doi:10.1039/d0tb02877k

CrossRef Full Text | Google Scholar

Yu, Y., Zhang, Z., Wang, Y., Zhu, H., Li, F., Shen, Y., et al. (2017). A New NIR-Triggered Doxorubicin and Photosensitizer Indocyanine green Co-delivery System for Enhanced Multidrug Resistant Cancer Treatment through Simultaneous Chemo/photothermal/photodynamic Therapy. Acta Biomater. 59, 170–180. doi:10.1016/j.actbio.2017.06.026

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, H., Sun, Y., Huang, R., Cang, H., Cai, Z., and Sun, B. (2018). pH-Sensitive Prodrug Conjugated Polydopamine for NIR-Triggered Synergistic Chemo-Photothermal Therapy. Eur. J. Pharmaceutics Biopharmaceutics 128, 260–271. doi:10.1016/j.ejpb.2018.05.013

CrossRef Full Text | Google Scholar

Zhao, P., Jin, Z., Chen, Q., Yang, T., Chen, D., Meng, J., et al. (2018). Local Generation of Hydrogen for Enhanced Photothermal Therapy. Nat. Commun. 9 (1), 4241. doi:10.1038/s41467-018-06630-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhao, T., Qin, S., Peng, L., Li, P., Feng, T., Wan, J., et al. (2019). Novel Hyaluronic Acid-Modified Temperature-Sensitive Nanoparticles for Synergistic Chemo-Photothermal Therapy. Carbohydr. Polym. 214, 221–233. doi:10.1016/j.carbpol.2019.03.043

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhi, D., Yang, T., O'Hagan, J., Zhang, S., and Donnelly, R. F. (2020). Photothermal Therapy. J. Controlled Release 325, 52–71. doi:10.1016/j.jconrel.2020.06.032

CrossRef Full Text | Google Scholar

Zhu, D., Fan, F., Huang, C., Zhang, Z., Qin, Y., Lu, L., et al. (2018). Bubble-generating Polymersomes Loaded with Both Indocyanine green and Doxorubicin for Effective Chemotherapy Combined with Photothermal Therapy. Acta Biomater. 75, 386–397. doi:10.1016/j.actbio.2018.05.033

PubMed Abstract | CrossRef Full Text | Google Scholar