Multifunctional CuBiS2 Nanoparticles for Computed Tomography Guided Photothermal Therapy in Preventing Arterial Restenosis After Endovascular Treatment

Chronic inflammation mediated by artery infiltrated macrophages plays critical role in artery restenosis after endovascular therapy. Evidence has demonstrated the potential ability of photothermal therapy (PTT) in eliminating chronic inflammation by targeting inflammatory cells including macrophages. Recently, increasing attention has been payed to copper chalcogenide nanocrystals doped of radiocontrast agent, e.g., bismuth (Bi) for computed tomography (CT) guided PTT. However, the application of imaging guided PTT in preventing artery restenosis is lacking and limited. Herein, a novel multifunctional CuBiS₂ nanoparticles (CuBiS₂ NPs) were synthesized for CT imaging guided PTT in artery re-stenosis prevention. The optimum amount and other conditions of CuBiS₂ NPs were optimized to exert the maximum ablation effect on macrophages with good biocompatibility. In vivo carotid injury model revealed that CuBiS₂ NPs exhibited promising therapeutic effect on inhibition of artery stenosis by eliminating macrophages with excellent CT imaging ability. The recent study highlights a new cost-effective metal nanostructures-based nanotechnology in prevention of artery restenosis after endovascular therapy.

Introduction

Atherosclerosis remains a devastating disease affecting cardio/cerebrovascular system with high morbidity and mortality (Toyohara et al., 2020). Although endovascular treatment i.e., percutaneous transluminal angioplasty (PTA) and stent implantation is somehow considered the treatment of choice when dealing with atherosclerotic stenosis or occlusion (Baumgartner et al., 2018), it would unavoidably lead to the injury of arterial endothelium, release of various cytokines and chemokines, and subsequent recruitment of circulating monocytes to the arterial wall (Koelwyn et al., 2018; Morley et al., 2018). Once activated, infiltrated monocytes differentiate into macrophages, mediating chronic inflammation of the injured artery (Koelwyn et al., 2018). Evidence has been growing that inflammatory macrophages play crucial role in artery restenosis after endovascular treatment (Sun et al., 2016; Pei et al., 2019). Infiltrated macrophages secrete matrix metalloproteinase (MMP) to degrade extracellular matrix and pro-inflammatory cytokines to stimulate the proliferation and transformation of smooth muscle cell (SMC) via different signaling pathways, eventually leading to the negative remodeling of the artery, i.e., artery restenosis (Yamashita et al., 2015; Zhao et al., 2017). Therefore, depletion of local aggregated inflammatory macrophages would provide a powerful mean to prevent artery restenosis.

Due to excellent properties of tissue penetration and minimally invasive, near-infrared (NIR) light driven photothermal therapy (PTT) based on nanomaterials could induce apoptosis and cell death by targeting intracellular protein and DNA (Al-Barram, 2020; Zhang et al., 2020; Zhi et al., 2020). With the advantages above and novel nanoparticles being developed (such as modified/decorated metal-organic framework based nanomaterial), nanotech PTT has been shown to be greatly applied in the anti-tumor theranostics, cardiovascular diseases and nervous system diseases, etc. (Shan et al., 2018; Luo et al., 2019; Wang et al., 2019; Pan et al., 2020; Zhou et al., 2020). Using ZD2-engineered gold nanostar@ metal-oragnic framework nanoprobes, it has been reported that the nanoprobes based PTT was efficient in treating triple-negative breast cancer with good magnetic resonance imaging property (Zhang et al., 2018). Nevertheless, we have previously reported that other nanoparticles, e.g., CuCo₂S₄ nanocrystals, MoO₂ nanoclusters, Fe₃S₄ nanoparticles, polypyrrole nanoparticles, and gold nanorods as PTT agents, can effectively alleviate inflammation by eliminating inflammatory macrophages (Peng et al., 2015; Qin et al., 2015; Wang et al., 2019; Zhang et al., 2019). Collectively, growing evidence suggest that nanoparticles as PTT agents may be an attractive and promising therapeutic target for chronic inflammation, including artery re-stenosis. However, the potential use of nanoparticle-based PTT in preventing artery restenosis after endovascular intervention is still lacking.

Metal sulfides are kinds of ideal candidate materials for photothermal applications, but their band gaps are usually too large to absorb significant fractions of NIR light and it has been gathering interest that by means of combining Cu⁺ and Bi³⁺, the ternary sulfides CuBiS₂ is formed (Dufton et al., 2012). CuBiS₂ is a semiconductor material with an energy gap distributed between 1.5 and 2.1 eV with good light absorption capability. Askari and Askari (2019) reported that CuBiS₂ demonstrated good photothermal property and showed anticancer effect on AGS cancer cell line via apoptosis pathway in vitro. On one hand, due to non-carcinogenic or toxic properties, bismuth is considered safe in pharmaceutical application and some of its components or compounds as an anti-inflammatory, antiviral and antifungal agents are widely used in clinical treatment (Tiekink, 2002; Iuchi et al., 2008). Moreover, accumulating evidence has been reported that by doping of radiocontrast agent, e.g., bismuth, copper chalcogenide nanocrystals exhibited excellent property for CT guided PTT, which has attracted great attention (Liu et al., 2015). However, evidence of CuBiS₂ based PTT in preventing artery re-stenosis is lacking.

In current study, CuBiS₂ nanoparticles (CuBiS₂ NPs) were synthesized and characterized in vitro/vivo and photothermal properties of the nanoparticles were further evaluated. Moreover, the cytotoxicity and PTT effect of CuBiS₂ NPs on inflammatory macrophages were also assessed in vitro. Carotid inflammation/endothelium injury model was established by using 29G Syringe needle mimicking endothelia damage after endovascular intervention. After that, PTT was conducted based on local injection of CuBiS₂ NPs to the surrounding of the injured carotid artery. Of note, we specially evaluated the imaging ability of CuBiS₂ NPs for in vivo tracking by small animal CT device. Histologic analysis demonstrated the effect of PTT in attenuating artery wall inflammation and stenosis. Also, histologic analysis of major organs and blood examination were performed to evaluate the biocompatibility of CuBiS₂ NPs.

Materials and Methods

Materials

Raw264.7 (mouse macrophage) was obtained from Fuheng Cell Bank, Fudan University (Shanghai, China) for the in vitro study. High glucose (4,500 mg^–1 mL) Dulbecco′s Modified Eagle′s Medium (DMEM), penicillin/streptomycin and fetal bovine serum were purchased from Gibco (Carlsbad, CA, United States). The CD68 antibody and corresponding 2nd antibody were purchased from Thermo Fisher Scientific (United States). Cell Counting Kit-8 (CCK-8) and Calcein-AM/PI Double Stain Kit were purchased from Thermo Fisher Scientific (United States).

CuBiS₂ NPs Synthesis and Characterization

One-step hydrothermal process (Wang et al., 2019) was used for the synthesis of the CuBiS₂ NPs according to the method described previously. Briefly, 0.256 g CuCl₂.2H₂O and 0.16 g BiCl₃ was mixed and stirred with 30 mL anhydrous alcohol and 50 mL glycerol by magnetic stirrer, during which thiourea resolution (i.e., 0.19 g thiourea dissolved in 10 mL anhydrous alcohol) was dropped and stirred together for 10 min. After that, the mixture solution was transferred to a flat flask for constant temperature water bath heating at 60°C for 1 h and moved to a Teflon lined autoclave to be heated at 160°C for 12 h. After centrifugation (5,000 r/min), the precipitate was then collected and washed with 75% ethanol and deionized water three times.

Scanning electron microscopy (SEM) characterized the size and morphology properties of the CuBiS₂ NPs. UV-vis absorption spectra were measured by Jasco V-7000 UV-visible-NIR spectrophotometer (Tokyo, Japan). X-ray diffractometer (XRD) analysis were conducted using Aeris X-ray diffractometer (Malvern, United Kingdom). Fourier transform infrared (FTIR) spectra were analyzed by KBr pellet methods using TruDefender^TM FTX/FTXi infrared spectrometer (Thermo Fisher Scientific; United States).

The power of 808 nm semiconductor laser (Hangzhou Qiulai Optoelectronics Technology Co. Ltd., China) could be externally adjusted (average 1.5 W). Calibration of the output power of lasers was conducted by using a hand-held optical power meter (Newport model 1918-C, CA, United States).

Cell Culture and Characterization

Raw264.7 macrophages were cultured in DMEM medium (4,500 mg^–1 mL glucose, with 10% FBS and 1% streptomycin/penicillin) and maintained at 37°C in a humidified 5% CO₂ atmosphere. Cellular immunofluorescence (IF) staining was performed to identify Raw264.7 macrophage properties by the fluorescence microscope (Olympus, Japan).

Cytotoxicity and Cell Viability

The cytotoxicity of CuBiS₂ NPs on macrophages was evaluated in the absence of PTT. Raw264.7 was co-cultured with CuBiS₂ NPs at different concentrations (0, 80, 160, 320 mg/mL) for 12 h. CCK-8 cell proliferation assay was used to measure the cell viability of macrophages after co-incubation with CuBiS₂ NPs, following which the safe concentration of CuBiS₂ NPs was determined and utilized for the subsequent in vitro or in vivo experiments. Next, the photothermal effects of CuBiS₂ NPs on macrophages was assessed. Raw264.7 was co-cultured with CuBiS₂ NPs at a predetermined concentration above for 12 h and subjected to 808 nm NIR laser irradiation for 5 min. After that, the irradiated macrophages stained with Calcein AM/PI (Calcein AM labeled-green, PI labeled-red, respectively) were observed under fluorescence microscope to discriminate living and dead cells. Flow cytometry (FCM) was performed to assess cell apoptosis analysis after Annexin V/PI staining following the procedures described previously (Wang et al., 2019).

Intracellular SEM

CuBiS₂ NPs engulfed by macrophages was observed by intracellular Transmission Electron Microscope (TEM; JEM-1400, Tokyo, Japan).

Animal Model and Photothermal Therapy

The animal experiment protocol was approved by Ethics Review Committee of Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine. Male, 8-week-old apolipoprotein E knockout mice (ApoE^–/– mice; Shanghai Southern Model Biological Co., Ltd.) were raised under specific pathogen-free (SPF) conditions. The method for artery inflammation and endothelium injury mice model is briefly described as follows: First anesthetize the ApoE^–/– mouse by intraperitoneal injection of pentobarbital sodium (40 mg/kg), after that the left common carotid artery (CCA) was dissected, and blocked by a blocking clamp at the proximal end, an incision was then made at the distal end of CCA, following which a 29G needle (BD Insulin Syringe Ultra-Fine^®) was inserted to the CCA. To mimic the endothelium injury by endovascular treatment, the needle in CCA was rotated for three circles and pushed forward-back for three times. After closing the incision, the carotid artery was sheathed with a constrictive silica collar as previously descripted (Wang et al., 2019).

After 14 days, 100 mL (160 mg/mL) CuBiS₂ NPs were injected into the left carotid arteries, while the contralateral right carotid artery was sham-operated to serve as intra-animal control (without silica collar). Twelve hours after injection, all the necks were irradiated at 808 nm NIR laser with power density of 0.5 W cm^–2 for 5 min. GX-300 photothermal medical device was used to record the temperature of full-body infrared thermal images dynamically.

Computed Tomography in vivo

Small animal CT scanning was used to determine the imaging ability of CuBiS₂ NPs. Endothelium injury model mice received CT scanning before and after perivascular injection with the CuBiS₂ NPs (160 mg/mL, 100 mL, per mouse). CT data were acquired using X-ray voltage biased to 50 kVp with a 670 μA anode current with projection angles of 720°. Afterward, 3-dimensional CT imaging was established to observe the distribution of CuBiS₂ NPs around the left carotid artery.

Tissue Histological Findings and Blood Examination

Fourteenth day after PTT, all the mice were sacrificed for histopathological examination. Both sham-operated and collared carotid arteries were harvested. The tissue infiltrating macrophages was stained for its surface maker CD68 and the immunofluorescent signal was detected by microscope which was further quantified using Image-Pro Plus software. The number of infiltrated macrophages were counted by two investigators blinded to group information. Hematoxylin-eosin (HE) staining was performed to determine the thickness of the intima-media of arteries by the Image-pro Plus software.

To assess the biocompatibility and toxicity of the CuBiS₂ NPs in vivo, major viscera, e.g., heart, liver, spleen, lung and kidney were made at 4–6 nm sections slides for HE staining. Five age-sex matched healthy ApoE^–/– mice were sacrificed as control. Biochemical parameters of the blood samples were measured in Shanghai Research Center.

Statistics Analysis

Quantitative data was represented as means ± standard deviation (SD), and one-way Annona analysis was used to compare the difference among multiple groups. Student’s t-test was used when appropriate. A P-value < 0.05 was considered statistically significant. All data are representative of at least three independent experiments.

Results and Discussion

Characterization of CuBiS₂ NPs

CuBiS₂ NPs were synthesized according to one-step hydrothermal method described previously. Figure 1A shows the XRD pattern of the as-synthesized products. The pattern of the sample can be matched well with the emplectite CuBiS₂ phase (JCPDS no. 43-1473), without no other peaks. EDS analysis (Supplementary Figure S1) showed that the products was composed of three elements (i.e., Cu, Bi, and S), further indicating the high purity of the CuBiS₂ NPs. As shown in Figure 1B, SEM results confirmed the products were nanoparticles with an average diameter of 240 nm (range from 180 to 400 nm). Figure 1C exhibits the UV-vis absorbance spectrum of the aqueous dispersion of CuBiS₂ NPs. It showed an intense absorption band centered at 910 nm. The strong NIR absorption made the nanoparticles possess the potential of to be PTT agents. Moreover, the zeta potential of CuBiS₂ NPs (160 mg/mL) was recorded by zeta potential analyzer (Nicomp Z3000) for 5 min, the result of which showed that the average zeta potential value was -1.86 mV.

FIGURE 1

Figure 1. Characterization of CuBiS₂ NPs. (A) XRD pattern and the corresponding standard pattern of CuBiS₂ NPs. (B) SEM images of CuBiS₂ NPs, Scale bars: 2 μm; (C) UV-vis-NIR absorption spectrum of CuBiS₂ NPs.

As an important member of mononuclear phagocytic system, macrophages have powerful phagocytosis. Therefore, before determining the cytotoxicity and photothermal properties of CuBiS₂ NPs on Raw 264.7, we explored the phagocytosis of macrophages toward the CuBiS₂ NPs by TEM. The results showed efficient phagocytosis of CuBiS₂ NPs with no obvious accumulation or sever damage to other organelles of macrophages (Figure 2).

FIGURE 2

Figure 2. Representative of TEM images of Raw264.7 co-incubated with CuBiS₂ NPs. Scale bar: 500 nm. (A,B) Macrophages incubated with 160 mg/mL CuBiS₂ NPs for 6 and 12 h, respectively. Scale Bar: 500 nm.

To evaluate the photothermal effect of CuBiS₂ NPs, the temperature evolution at different CuBiS₂ NPs concentration (0, 80, 160, 320 mg/mL) under continuous 808 nm wavelength laser irradiation for 300 s were recorded, showing that the temperature was elevated in dramatic and smooth pattern with increasement of CuBiS₂ NPs concentration (Figure 3A). Cytotoxicity of CuBiS₂ NPs on macrophages should be considered before other biomedical applications. To this end, Raw264.7 was co-cultured with CuBiS₂ NPs for 12 h in the absence of PTT. Subsequently, the CCK-8 assay was conducted to measure the concentration-dependent cytotoxic effect of CuBiS₂ NPs on Raw264.7 (Figure 3B). Finally, no significant difference on cell cytotoxicity was identified between the CuBiS₂ NPs group and the control group below the concentration of 160 mg/mL, while significant cytotoxicity was observed when macrophages were co-incubated with CuBiS₂ NPs at concentration above 160 mg/mL, indicating that CuBiS₂ NPs exhibited good biocompatibility at concentration of 160 mg/mL (Figure 3B).

FIGURE 3

Figure 3. Screening the optimal concentration of CuBiS₂ NPs for photothermal therapy. (A) In vitro photothermal effect of PBS and CuBiS₂ NPs in aqueous solution at different concentrations. (B) Relative cell viabilities of macrophages after co-incubation with CuBiS₂ NPs at different concentrations for 12 h without photothermal therapy. (C) Relative cell viabilities of macrophages after co-incubation with CuBiS₂ NPs at different concentrations for 12 h under photothermal therapy.

To investigate the applicability of CuBiS₂ NPs based PTT, in vitro evaluation of their photothermal efficacy was performed. Raw264.7 macrophages were co-cultured with CuBiS₂ NPs at 160 mg/mL for 12 h, and then subjected to 808 nm NIR irradiation (0.3 W/cm²). Calcein AM/PI results demonstrated that few dead cells were observed in the control group, and CuBiS₂ NPs group (Figures 4A,B). While about 60% dead cells were observed in the CuBiS₂ NPs + PTT group (Figure 4C). In accordance with Calcein AM/PI, the CCK-8 assay showed similar results (Figure 3C). The above results showed that CuBiS₂ NPs based PTT exhibited excellent photothermal property with remarkable macrophages death being observed.

FIGURE 4

Figure 4. Photothermal effect of CuBiS₂ NPs on macrophages by Calcein AM/PI and flow cytometry. (A–C) Representative images of live (green) or dead (red) cells without or with CuBiS₂ NPs co-incubation (160 mg/mL), respectively. Scale Bar: 100 μm.

Apoptosis is an important type of programmed cell death which can be induced by thermal effect, whether it is involved in CuBiS₂ NPs based PTT induced cell death is unknown. Annexin V/PI duo-stanning detects the signals of Annexin V and the impermeable nucleic acid dye, respectively, which can quantify the proportion of mid- and late-apoptotic cells, we used Annexin V/PI stanning to determine the CuBiS₂ NPs based PTT effect on macrophages and discriminate cell apoptosis and necrosis. Toward this end, FCM was performed showing that compared with CuBiS₂ NPs group, CuBiS₂ NPs + PTT group exhibited a significantly higher either early apoptosis (18.3 vs. 0.21%) or mid/late apoptosis (60 vs. 5.9%) index of macrophages with statistical difference (Figure 5). In contrast to programmed cell death, necrosis is the death process in which cells are subject to strong physical and chemical or biological factors that cause disordered changes in cells. As shown in Figure 5, the necrosis index of macrophages was identical between CuBiS₂ NPs group and CuBiS₂ NPs + PTT group, indicating that the type of CuBiS₂ NPs based PTT induced macrophages death was mainly apoptosis instead of necrosis. The above results demonstrated that CuBiS₂ NPs based PTT could ablate macrophages effectively by inducing cell apoptosis (Figures 5A–C), which possesses great potential to alleviate chronic inflammation mediated by macrophages.

FIGURE 5

Figure 5. CuBiS₂ NPs based PTT induced apoptosis of macrophages. Flow cytometry analysis of Raw264.7 apoptosis after different treatments: (A) Control; (B) CuBiS₂; (C) CuBiS₂ + PTT. Cell apoptosis was detected by flow cytometry with annexin-V-FITC and PI. CuBiS₂ NPs + PTT group showed higher early (Q7) and mid/late (Q6) apoptosis index than CuBiS₂ group.

CT Imaging Assessment and Photothermal Effect of CuBiS₂ NPs in vivo

Nanomaterials have drawn great attention over the decades and been widely utilized in biomedicine (Cole et al., 2015; Zhang et al., 2016). Nanoplatforms can integrate imaging moieties and therapeutic species flexibly, which has been widely applied in cancer diagnosis and treatment (Liu et al., 2007). Among numbers of investigations, CT imaging guided PTT has been widely reported and highly recognized. Conventional contrast agents have the disadvantages of short imaging time and potential nephrotoxicity, while new nanocrystal contrast agents such as Bi₂S₃, TaOx, and other nanoparticles (NPs) overcome the above shortcomings and has high absorption coefficient (Ai et al., 2011; Lee et al., 2012; Leeuwenburgh et al., 2013; Cheng et al., 2014). With high density (ρ) and atomic number (Z), Bi element was reported to possess high attenuation coefficient of X-ray (Elsabahy et al., 2015). With long vascular half-life, Bi₂S₃ nanoplates was reported to gain considerable potential to achieve enhanced CT efficacy with lower agent dose in future clinical use (Rabin et al., 2006). In addition to the photothermal ablation ability, CT imaging property of CuBiS₂ NPs was further assessed in intro and in vivo on the bases of pre-determined concentration for cytotoxicity and cell viability evaluation in vitro. Figure 6 presents the CT image of aqueous dispersions of the CuBiS₂ NPs with different concentration, showing that CT signal intensity enhanced with increased concentrations of the CuBiS₂ NPs. Meanwhile, the Hounsfield units (HU) values increased linearly with the concentration of the CuBiS₂ NPs. As shown in Figures 6A,B, CuBiS₂ NPs at concentration of 160 mg/ml exhibited excellent in vitro imaging property, which was further tested in vivo showing that distinct difference was observed in CT images of CuBiS₂ NPs surrounding inflammatory artery, demonstrating good CT imaging property of CuBiS₂ NPs (Figures 6C,D).

FIGURE 6

Figure 6. Demonstration of computed tomography imaging property of CuBiS₂ in vitro and in vivo. (A) The CT signals intensity of different CuBiS₂ concentration in vitro. (B) CT value (HU) of the CuBiS₂ NPs as a function of the concentration of NPs. (C,D) 3-D CT imaging of mice without and with CuBiS₂ NPs being injected to the surrounding of the carotid artery, respectively.

Endovascular repairs would unavoidably cause mechanical injury to endothelial cells and activates the endothelium to express massive adhesive molecules and chemokines, which promotes the recruitment of monocytes, accelerates differentiation of monocytes into macrophages and aggravate artery inflammation, leading the restenosis after endovascular treatment (Sun et al., 2016; Pei et al., 2019). According to above results, we speculate that the macrophages infiltrated in the injured lesion of artery endothelium engulf locally injected non-toxic CuBiS₂ NPs and provide opportunities for in vivo non-invasive PTT to attenuate arterial inflammation and stenosis. Toward this end, a carotid artery inflammation/endothelium injury model was conducted in ApoE^–/– mice by mechanical injury to intima using a 29G Syringe needle mimicking endovascular treatment related endothelium injury to further validate the feasibility of CuBiS₂ NPs based PTT for inhibiting artery restenosis (Supplementary Figure S2). The mice received local injection of a solution of the CuBiS₂ NPs (160 mg/mL, 100 mL) around the left carotid artery and subjected to NIR laser irradiation (808 nm, 0.5 W cm^–2) 12 h. An equivalent volume of PBS was injected as control. The local temperatures of the left necks were recorded by an infrared thermal camera dynamically. In mice injected with CuBiS₂ NPs, the local surface temperature of the left neck gradually increased to 45°C rapidly within 5 min, while non-dramatic increase of the local surface temperature was recorded in the control group being injected PBS (Figure 7). After 14 days, the mice were sacrificed, and the carotid arteries were collected for HE staining and immunofluorescence examination.

FIGURE 7

Figure 7. In vivo thermal effect of CuBiS₂ NPs in vivo. (A,B) Representative of infrared thermal images of the mice after being injected with PBS or CuBiS₂ NPs under laser irradiation. (C) Temperature changes according to irradiation time in corresponding squared regions (red: PBS control; black: CuBiS₂ NPs group).

The effectiveness of CuBiS₂ NPs based PTT for alleviating arterial inflammation was further assessed by IF (Figures 8A–D), the results of which showed that the artery-infiltrating macrophages in the CuBiS₂ NPs + PTT group reduced greatly. The HE staining exhibited that the thickness of arterial intima-media in the CuBiS₂ NPs + PTT group was much thinner than that of the CuBiS₂ NPs group and the control group (Figure 8E). However, there was no significant difference between the control group and the CuBiS₂ NPs group. Thus, the results suggested CuBiS₂ NPs as photothermal agents and 808 nm NIR PTT can effectively eliminate inflammatory macrophages infiltrated in the layer of artery to inhibit arterial inflammation and stenosis.

FIGURE 8

Figure 8. CuBiS₂ NPs based PTT mitigated arterial inflammation in vivo. (A–C) Representative of HE and Immunofluorescence stanning of carotid artery from control group, CuBiS₂ group and CuBiS₂ + PTT group, respectively. (D,E) Representative of CD68 + macrophages positive area and artery wall thickness in each group, respectively. Scale bar: 50 μm.

Biocompatibility of the CuBiS₂ Nanoparticles

Of note, favorable biocompatibility of NPs should be considered and guaranteed for living body. Mice from CuBiS₂ NPs group and control group (n = 4, each) were sacrificed 14 days after PTT. Paraffin-embedded sections (4 nm) of major organs were stained with hematoxylin-eosin (HE) dye. No obvious histopathological changes were found between the two groups (Figure 9A). No obvious cell degeneration and necrosis were observed in major viscera (Figure 9A). Blood samples were tested using Elisa method. Statistical difference was not gained between the two groups in terms of alanine aminotransferase (ALT), asparate aminotransferase (AST), blood urea nitrogen (BUN), or creatinine (Cr) (Figure 9B). As dysfunction of endothelial cells contributed to negative remodeling the artery (e.g., artery restenosis), Evan’s Blue stanning of the carotid artery from each group was performed to test the potential side-effect of CuBiS₂ NPs on reendothelialization, showing that no difference was found between the groups (Supplementary Figure S3). These results show that CuBiS₂ NPs as PTT agents have no toxic effects on the major organ function, and thus are safe for use in vivo.

FIGURE 9

Figure 9. In vivo biocompatibility test of CuBiS₂ NPs. (A) HE staining of major viscera showed no obvious histopathological changes in heart, intestine, kidney, liver, lung, or spleen between the control group and the CuBiS₂ group. Scale bar: 50 μm. (B) Laboratory blood test of control group and CuBiS₂ group at different time points (3, 7, 14 days) after CuBiS₂ NPs injection, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (CR).

Conclusion

In summary, CuBiS₂ NPs were successfully synthesized by one-step hydrothermal method. The NPs showed intense NIR absorption due to the defect structure, thus were demonstrated excellent photothermal performance. Due to the High X-ray attenuation coefficient of bismuth, CuBiS₂ NPs possessed CT imaging ability. CuBiS₂ NPs based PTT could eliminate macrophages in vitro and in vivo, expanding the understanding of CuBiS₂ NPs as efficient PTT agents for arterial inflammation and restenosis after endovascular treatment.

Data Availability Statement

All datasets generated for this study are included in the article/Supplementary Material.

Ethics Statement

The animal study was reviewed and approved by the Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine.

Author Contributions

XW and XL contributed to the model development, code development, data generation and analysis, writing, and editing the manuscript. JL and GY contributed to the model development, data analysis, and editing the manuscript. KL and RW contributed to the model development, data analysis, writing, and editing the manuscript. All the authors contributed to discussion of the results.

Funding

This work was supported by the National Natural Science Foundation of China (Grant Nos. 81701801, 81971712, 81970405, and 81801526) and Clinical Research Program of Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine (Grant No. JYLJ019).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fbioe.2020.585631/full#supplementary-material

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