Ida Sperle1,2*
Gyde Steffen1,3Siv Aina Leendertz1,3Navina Sarma4Sandra Beermann3,5Roma Thamm3,4
Yanita Simeonova1
Markus Cornberg6,7Heiner Wedemeyer6Viviane Bremer1Ruth Zimmermann1Sandra Dudareva1- 1Department of Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany
- 2Charité—Universitätsmedizin, Berlin, Germany
- 3Department of Infectious Disease Epidemiology, Translational Infrastructure Epidemiology of the German Centre for Infection Research, Robert Koch Institute, Berlin, Germany
- 4Department of Epidemiology and Health Monitoring, Robert Koch Institute, Berlin, Germany
- 5Centre for International Health Protection, Robert Koch Institute, Berlin, Germany
- 6Department of Gastroenterology, Hepatology and Endocrinology, Medizinische Hochschule Hannover, Hanover, Germany
- 7Thematic Translational Unit Hepatitis of the German Centre for Infection Research, Hanover, Germany
Background: One of the five strategic directions in the World Health Organization global health sector strategy on viral hepatitis 2016–2021 is to generate strong strategic information for focused action to understand the viral hepatitis epidemic and focus the response. Knowledge of national prevalence is a cornerstone of strategic information. Germany is considered to be a low prevalence country for viral hepatitis B, C, and D, however the prevalence is likely to be higher among at-risk groups.
Methods: The aim of this work was to give a detailed overview of the prevalence of viral hepatitis B (HBsAg, anti-HBc), C (anti-HCV, HCV RNA), and D (anti-HDV, HDV RNA) in different population groups in Germany. Therefore, we analyzed the results of a comprehensive literature search on various aspects of the epidemiological situation of hepatitis B, C, and D in Germany. Eligible publications including information on hepatitis B, C, and D prevalence were extracted from the overall spreadsheet table and summarized and analyzed based on virus and different population groups. A quality appraisal was performed using a checklist developed by Hoy et al. to assess risk of bias in prevalence studies.
Results: Overall, 51 publications were identified through the literature search. The overall prevalence of HBsAg in the general (and proxy) population ranged from 0.3 to 1.6%. Among at-risk groups, including clinical populations and health care workers, the HBsAg prevalence ranged from 0.2% (among rheumatic patients) to 4.5% among HIV positive patients. The overall prevalence of anti-HCV in the general (and proxy) population ranged from 0.2 to 1.9%. Among at-risk groups, including clinical populations and health care workers, the anti-HCV prevalence ranged from 0.04% (among health care workers) to 68.0% among people who inject drugs.
Conclusions: The hepatitis B and C prevalence in the general population in Germany is low. Prevalence is high to very high among at-risk populations, however for some groups evidence was incomplete or missing completely. To reach the elimination goals in Germany and implement a targeted response, more research among at-risk groups is needed.
Introduction
Viral hepatitis (VH) is a major global public health concern. Worldwide, an estimated number of 257 and 71 million people are chronically infected with hepatitis B virus (HBV) and hepatitis C virus (HCV), respectively (1). In total, 15–20 million people are infected with hepatitis D, which corresponds to 5% among those with hepatitis B (1). In the World Health Organization (WHO) European Region, an estimated 15 and 14 million people suffer from chronic HBV and HCV infection, respectively (2).
The WHO global health sector strategy for VH (2016–2021) (3), the WHO European level action plan (2016) (2) and the German integrated national strategy for HIV, HBV, and HCV and other sexually transmitted diseases (2016) (4) represent steps forward in terms of elimination of VH. Nevertheless, they shed light on the lack of comprehensive data to monitor progress and to identify where intensified efforts are needed.
The VH viruses, HBV, HCV, and HDV, show diversity in their prevalence, but also in their modes of transmission depending on country, context, and population group. Data on the country specific epidemic in Germany as well as on population groups most at risk and the effectiveness of prevention and treatment measures are urgently needed to intensify efforts and to reach the elimination goals by 2030.
The most recent national population-based survey among adults in Germany (2008–2011) (DEGS1) found a low HBV and HCV prevalence (0.3%) (5). However, it is known that the prevalence of VH is higher in some groups more vulnerable to VH infection. More research among population groups that are often poorly represented in population-based surveys and more vulnerable to VH (hereafter populations at-risk) is needed.
The aim was to create an overview of existing evidence on the epidemiology of HBV, HCV, and HDV in different population groups in Germany in the time period from 2005 to 2017 to serve as baseline information and guide to improve monitoring of VH in Germany. In this paper, the prevalence in Germany is described.
Materials and Methods
Review Process
The aim of the overall scoping review was operationalised into 13 specific research questions to identify available evidence in the form of published literature on VH epidemiology in Germany (6). One of the 13 questions was “What is the prevalence of HBV, HCV, and HDV in Germany?”
The detailed methods of the review are described elsewhere (6). In brief, the search and reporting methods were based on the reporting items for systematic reviews and meta-analysis extension for scoping reviews (PRISMA-ScR) and the Cochrane Collaboration (https://training.cochrane.org/handbook). Included in the review were available full-text (peer- and non-peer-reviewed) publications of original works in German or English language, published between 01/01/2005 and 09/03/2017 with end of data collection after 01/01/2005 and content relevant to one or more of the research questions. The literature search was conducted in six electronic databases (MEDLINE, EMBASE, Europe PMC, Scopus, Bielefeld Academic Search Engine (BASE), and CC Med) with a detailed search string developed from the research questions Supplementary S1. The final search was conducted on 09/03/2017. The reference list of all publications retrieved from the electronic search and eligible for full-text screening as well as national surveillance reports not cited in the six electronic databases were also screened for references of further publications meeting the inclusion criteria Supplementary S2.
The screening was performed on abstract and full-text level. After full-text screening, relevant information according to the research questions was extracted from the eligible publications using standardized extraction sheets. The screening and data extraction process was performed by two independent reviewers. All discrepancies between the reviewers were discussed. A validation of the screening and extraction process was conducted.
Data Analysis
The extracted data was assigned to different pre-defined categories based on the research questions and sorted by population groups using the definition of the target population in the corresponding publication. Population groups were defined based on the WHO guidelines on Hepatitis B and C testing (7) and adapted to the German context. Population groups were (a) the general population (GP), (b) sub-populations being representative of the national population, which are not considered at higher risk for VH and therefore act as a proxy for the GP (blood donors and pregnant women), (c) clinical populations [populations with non-VH related underlying disease and people with VH in hepatological care (PLWVH)], (d) populations at risk for VH due to risk behavior/exposure (household contacts of PLWVH, health-care workers (HCW), people living with HIV (PLWH), men who have sex with men (MSM), people in prison and closed settings, people who inject drugs (PWID), sex workers) or because they are part of a population with high VH seroprevalence (e.g., mobile or migrant populations from intermediate- and high-endemic countries). When no definition of the target population was available in the corresponding publication, the review team allocated the publication to a population group. In this paper, the evidence identified on HBV, HCV, and HDV prevalence is presented which includes all publications from the scoping review allocated to the category “prevalence.”
A quality of the evidence on prevalence was assessed using a checklist developed by Hoy et al. (8). This tool allows a judgement of the overall risk of bias based on the assessment of 10 individual items covering internal and external validity and reliability (8). The assessment was performed by one of the reviewers, and then checked by the other reviewer and categorized as either at “low risk” or “high risk” of bias. Discrepancies were discussed to reach agreement, and a third reviewer was consulted if needed. The publications were not weighted according to their quality of evidence in the analyses.
Results
Overall, the electronic literature search retrieved 18,410 publications, and an additional 14 publications were identified by manual search. After removal of duplicates, abstract and full-text screening 104 publications were included in the scoping review which covered all 13 research questions. Fifty-six publications of the 104 were allocated to the category “prevalence.” Five of 56 publications were excluded due to the lack of relevance for the analysis, and the remaining 51 were included (Figure 1). Some of the included publications reported on the prevalence of more than one pathogen or marker (Table 1).
Figure 1. Study flow of study selection.
Table 1. Number of publications.
The results of the quality appraisal performed for the publications included in this paper are summarized in Table 2. Fourteen of the 51 publications were assessed to have a high risk of bias due to either lack of properly describing their sampling and recruitment strategy, and/or that the VH markers were either not specified or that VH status was self-reported.
Table 2. Hepatitis B, C, and D prevalence in Germany.
Prevalence of Hepatitis B, C, and D in Germany
Of the 51 publications reporting on VH prevalence 37 had a cross-sectional design, eight a cohort design, five were surveillance studies, and one was a case-control study (37). For seven publications the origin of the data was national surveillance. National level data were reported by 16 publications, while regional or local level data were reported by the remaining publications, except one which did not report on which level the data were from (16).
Hepatitis B
The 39 publications covering HBV prevalence were on studies conducted between 1996 and 2016. The prevalence of HBV in the GP, including proxy populations, was reported in 13 publications of which 11 were at national level (5, 10, 13, 25, 27, 30–32, 35, 58, 59). One publication did not describe on which level the data were from (16).
The prevalence of HBsAg in the GP ranged from 0.3 to 0.7%, and 0 to 1.6% among proxy populations. The prevalence of anti-HBc ranged from 0.5 to 0.6% in GP, and 0.9 to 1.4% in proxy populations. Six publications (16, 18, 30–32, 35) included surveillance data among blood donors. Four of these reported on the prevalence of HBsAg, HBcAg, HBV-DNA (not separately) among first time blood donors and reported a range from 0.12 to 0.15%. Two studies described anti-HBc prevalence among first time blood donors and found a prevalence of 1.9% (18) and 0.9% (16).
Four studies described anti-HBc prevalence among HCWs which ranged from 0.5 to 1.7%, one identifying a self-reported anti-HBc prevalence among medical doctors (1.6%) (36).
One study included HBV infection among household contacts (partner and children) and reported a self-reported prevalence of 10.7% (17).
Thirteen studies looked at HBV prevalence among clinical populations, of which four were VH patients in hepatologic care. These four described the proportion of patients with HCV who were co-infected with HBV (markers not specified) which ranged from 0.1 to 39.1%. The prevalence of HBsAg was reported by eight studies and ranged from 0.2 to 3.4%. Four of these were among emergency and trauma department in which the prevalence ranged from 0.5 (anti-HBc and HBsAg) to 1.3%. One study reported an anti-HBc IgG prevalence of 8.3% among alcohol dependent patients (38), and one study an anti-HBc prevalence of 5.6% among patients with rheumatic disease (21) (Figure 2).
Figure 2. Hepatitis B prevalence in Germany by study population and marker, 2005–2017.
Eight publications described HBV prevalence in at-risk populations, and three were among people with migration background. One study was among refugees screened in an emergency department and found a prevalence of HBsAg and anti-HBc of 2.3 and 14.0%, respectively. The country of birth was not specified (24). Another study screened patients with migration background (patient or parents of patient) and found an HBsAg prevalence of 3.6% and anti-HBc of 32.5%. In total, 87.3% of the patients were from the Eastern Mediterranean Area, 12.0% were from Eastern Europe, and 0.7% originated from other countries (40). The third study tested 488 Syrian refugees upon arrival in Germany, but none were HBV positive (markers not specified) (23).
HBV prevalence among PLWH was reported by two studies, one of which was among HIV positive MSM. The prevalence of HBsAg was 4.5% among HIV patients (28) and 1.7% among HIV positive MSM (25).
Three studies included results on HBV prevalence among PWID. One study included results on self-reported HBV infection among PWID recruited from the streets or drug consumption rooms and from substitution clinics, and found a rate of 14.1 and 14.0%, respectively (41). One study reported an HBsAg prevalence of 1.3% among PWID in specialized methadone substitution centers (34) and another reported an anti-HBc prevalence of 25% (range in the cities: 4.6–33%), among which 1.1% were HBsAg positive (range in the cities: 0.3–2.5%). among PWID recruited from low threshold services (43) (Figure 3).
Figure 3. Hepatitis B prevalence in Germany by study population (at-risk) and marker, 2005–2017.
Hepatitis C
The 33 publications covering HCV prevalence were on studies conducted between 1996 and 2014. The prevalence of HCV in the GP, including proxy populations, was reported in 11 publications of which 10 were on the national level.
The anti-HCV prevalence in the GP ranged from 0.2 to 1.9%, and was 1.5% among baby boomers (proxy population) (42). Two studies reported an HCV RNA prevalence of 0.2% (5) and 0.4%, respectively (44).
Four publications (30–32, 35) reported on surveillance data among blood donors, describing an anti-HCV range from 0.06 to 0.08%.
Three studies on prevalence of anti-HCV among HCWs reported a prevalence of 0 and 0.03% (29, 33) and of 0.04% self-reported anti-HCV (36).
Ten studies analyzed HCV prevalence among clinical populations (26, 27, 37–39, 45, 46, 51, 52, 55), including one in HBV patients in care (55). Anti-HCV ranged from 0.2 to 5.2% with the lowest prevalence in autologous blood donors (giving blood for themselves). One study reported on HCV RNA among two groups of clinical patients and reported a prevalence of 2.4% among chronic haemodialysis patients and 4.6% among kidney transplant recipients (37). One study reported on the proportion of HBV patients with HCV without specifying the marker where 0% were co-infected with HCV (55). Six studies were among emergency and trauma department in which the anti-HCV prevalence ranged from 0.9 to 3.5% (Figure 4).
Figure 4. Hepatitis C prevalence in Germany by study population and marker, 2005–2017.
Nine publications reported HCV prevalence in at-risk populations. Two studies (40, 54) reported an anti-HCV prevalence among mobile/migrant populations of 1.9% among patients with migration background in eight primary care centers in Northwest Germany (40) and 0.4% among refugees and asylum seekers who went through routine screening for infectious diseases upon arrival in Germany (54). The first study with patients largely originating from the Eastern Mediterranean area (87.3%) followed by Eastern Europe (12.0%) and other countries (0.7%) also reported an HCV RNA prevalence of 0.7% (40). The country of origin of the refugees and asylum seekers in the second study was not described (54).
Three studies reported on HCV prevalence among PWID (34, 41, 43) in which the anti-HCV prevalence ranged from 63.0 to 68.0%. One cross-sectional study covered eight cities where the anti-HCV prevalence ranged from 36.9% in Leipzig to 73.0% in Hannover. The HCV RNA prevalence ranged from 23.1 to 54.0% (43).
One study included results on self-reported HCV prevalence among PWID recruited from the street and PWID in opioid-substitution treatment (OST) and found a prevalence of 58.3 and 58.7%, respectively (41). One nationwide study including 21 prisons found an HCV prevalence reported by prison physicians of 14.3% among people in prisons, of which 21.9% were PWID (48).
Three studies described prevalence among PLWH, and for two studies these were MSM. Among HIV positive patients the anti-HCV prevalence was 10.6% (28). Among MSM with HIV the anti-HCV prevalence was 8.2% (25). One study described self-reported HCV prevalence among MSM who were HIV positive and HIV negative (or untested) and the prevalence was 8.8 and 0.2%, respectively (47) (Figure 5).
Figure 5. Hepatitis C prevalence in Germany by study population (at-risk) and marker, 2005–2017.
Hepatitis D
Four publications covered HDV prevalence based on studies conducted between 1989 and 2011. All four included results on the prevalence in patients with chronic HBV, three recruited patients from hospital settings and in one physicians provided patient data (58). The overall prevalence of HDV ranged from 0 to 7.4%. One study specified the HDV marker and reported an anti-HDV prevalence of 7.4%, and HDV RNA of 64.5% (57). One study collected nationwide data from 74 centers across Germany with focus on hepatology and the prevalence was 1.4% in the population of HBV positives.
Discussion
The aim of this analysis was to assess the state of evidence on HBV, HCV, and HDV prevalence in Germany. To our knowledge this is the first time that all available evidence on HBV, HCV, and HDV prevalence has been systematically searched for. The results demonstrate that there is a large body of evidence on prevalence of HBV and HCV in Germany, but less on HDV.
The available evidence is highly variable. Good coverage was found for the GP and some clinical populations but there are gaps in knowledge for some at-risk populations and missing for sex workers, people who have received blood transfusion and persons with tattoos/piercings.
Prevalence in General Population
The low prevalence of HBV and HCV found in the GP is similar to what has been found in other European countries (60). A higher prevalence was found for proxy populations for the GP [e.g., some patient groups or among pregnant women compared to larger health examination surveys which use a random sample of the GP such as DEGS1 (5)]. The robustness of estimates based on proxy populations for the GP has its limitations. On the one hand, pregnant women may serve as a good proxy as women with migration background are likely to be better represented, however in some cases even over-represented, compared to the larger population-based surveys. On the other hand, they represent a group in more frequent contact with health care services and women of younger age only, and not all pregnant women attend all routine screenings potentially introducing selection bias.
A higher prevalence can be found in birth cohorts of the GP exposed through nosocomial or transfusion-related transmission. These are often referred to as baby boomers. Although the epidemiology is changing with injecting drug use now being a primary risk factor, the prevalence of VH is associated with age and sex. A higher prevalence is often found among males and with increasing age (5, 61, 62), and using baby boomers as proxy population for the GP in Germany should be carefully considered. The study which compared baby boomers and GP in this study found similar prevalence of HBsAg prevalence in the two groups, but higher anti-HCV prevalence among the German baby boomer population (42). Data from first time blood donors were included as this group is more likely to resemble the GP compared to multiple blood donors.
The prevalence of HDV was above 5% in two of the four studies identified. While HDV is relatively rare compared to HBV and HCV, as it requires the envelope of HBV for its entry into hepatocytes, it has important implications for mortality and morbidity (56). The prevalence of HDV found in this review is similar to that in other European countries (e.g., Spain (4.0%) (63), and Switzerland (4.4%) (64), but lower than for example in Italy with 11.9% (65)). A recent systematic review found that globally 10.6% of HBsAg carriers without risk factors (IDU or high risk sexual behavior) are infected with HDV, but higher prevalences were found among those with risk factors with 37.6% in PWID and 17.0% in populations with high risk sexual behavior (66).
Improving screening for people with migration background from areas of high prevalence (e.g., from Turkey, who represent the majority of migrant populations in Germany and which is a high prevalence area (67)), may improve early diagnosis, treatment, and data on HDV in Germany.
Prevalence in At-Risk Populations
The VH burden disproportionately affects some population groups more (61) which was also confirmed in this review.
Sexual transmission of HBV is more common than of HCV, whereas HCV is largely transmitted via blood-to-blood contact with infected fluids. The most common transmission paths ultimately affect which groups are at highest risk and where there is the highest prevalence (68, 69).
The HBV prevalence among populations with migration background was higher than in the GP among refugees who were screened in an emergency department (country of origin not specified) (24), and among patients with migration background primarily from the Eastern Mediterranean Area and Eastern Europe (40). The study among refugees arriving from Syria, where none were tested positive for HBV (23) was among unaccompanied minors who may have a different prevalence than the adult population.
The reasons for a higher prevalence found in the two studies are likely multifacetted. Firstly, people with migration background and refugees are two groups of people that need to be distinguished. Refugees are more likely to have been exposed to risks during flight from war and or persecution in home country and to have lack of access to well-functioning health care services and timely medical care. For people with migration background, prevalence will depend in part on the prevalence in the country of origin. This was however only described in two of three studies (23, 40). The wide ranges of prevalence (from 0 to 3.6%) found in this review coincide with results from other European countries demonstrating large heterogeneity depending on country of origin, ranging from 0 to 22.2% among mobile/migrant populations with the highest prevalence reported among migrants from countries in Southeast Asia (20%) and Sub-Saharan Africa (22.2%) (70). The highest rates of prevalence were found among refugees from east European (1.6–53.1%) and Southeast Asian countries (57.7%) (70).
For HCV, a relatively lower prevalence than HBV and closer to that of the GP was found for people with migration background. One study (not with specific focus on people with migration background) looking at HCV prevalence among patients arriving at an emergency room observed that 67.8% of those HCV positive were of German origin (51).
In one study of 236 refugees and asylum seekers screened for anti-HCV at a reception center upon arrival in Germany, one tested anti-HCV positive (54) (country of origin not specified), and in the other 1.9% were anti-HCV positive among 1,298 people with migration background, primarily from the Eastern Mediterranean Area and Eastern Europe, tested in primary care centers (40). The most HCV affected regions are the WHO Eastern Mediterranean and European Regions (71), corresponding to the higher prevalence found among the people from the Eastern Mediterranean Area and Eastern Europe. HCV estimates from other European countries range from 0 to 19.2% with the highest prevalence rates reported among migrants from countries in Eastern Europe (9.3%) and Sub-Saharan- Africa (19.2%). Among refugees, the highest rates were found among refugees from South Asia (9.1%) and Sub-Saharan Africa (26.7%) (70).
Factors relating to higher vulnerability as a result of a migration background are intertwined and related to social and political factors, either in the country of origin or the new country. Further, drawing any general conclusions for migrants based on this review is challenging. The country of origin differed in the included publications ranging from patients with parents with migration background to newly arrived refugees from Syria. Moreover, the publications that reported on prevalence among mobile/migrant populations categorized the countries/regions of origin differently. A standardization of countries/regions of origin reported in literature would improve the comparison across countries and over time to improve the understanding of the epidemic. Moreover, strengthening the terminology is crucial, as different terminology has very distinct and different meanings, and confusing these terms (e.g., migrant vs. refugee, or nationality vs. country of residence) hinders standardization of data and generation of comparable estimates.
More efforts are needed to reach migrant/mobile populations in the larger health surveys conducted in Germany and to include VH testing in these larger population-based surveys. This is currently being piloted and planned to be implemented at the Robert Koch Institute (RKI) as part of the Improving Health Monitoring in Migrant Populations (IMIRA) Project (72, 73).
People living with HIV (PLWH) are also disproportionately affected by VH, and higher rates of HBsAg prevalence was found among PLWH in this review. Sexual transmission of HBV may occur in particular among MSM and/or heterosexual persons with multiple sex partners, making the interaction between different at-risk groups important to consider.
A higher prevalence of HCV among PLWH was also found, which mirrors the global pattern where a 5.8 times (95% CI 4.5–7.5) increased odds of HCV antibody positivity in HIV-positive people compared with HIV-negative people across all population groups has been documented (74). There is particularly a high rate among at-risk groups with rates as high as 6.4% in MSM and 82.4% in PWID. Sexual behaviors linked to blood exposure and use of drugs may contribute to the high prevalence among MSM and HIV positive MSM. Chemsex, referring to voluntary intake of psychoactive and non-psychoactive drugs to facilitate and/or enhance sexual intercourse mostly among MSM, has been shown to be associated with higher risk of HIV and HCV transmission and contribute to increased risk among MSM (75).
High prevalence rates of HBV and HCV were shown among PWID in this analysis, corresponding to rates found in the EU/EEA ranging from 0.5 to 6.1% (HBsAg) and 13.8 to 84.3% (anti-HCV), respectively (61).
This coincides with the pattern of IDU being the main driver of HCV transmission in Europe accounting for more than 40% of new reported infections where the transmission route is known (76). A recent modeling study found that if the increased risk of HCV transmission among PWID was removed, an estimated 43% (95% CrI 25–67) of incident HCV infections globally would be prevented from 2018 to 2030 (77), and the population attributable fraction was higher in high-income countries. Focusing on prevention, testing, and treatment of PWID is important in targeted settings as part of harm reduction services.
In total, 14.3% of the prison population throughout Germany were anti-HCV positive (48). In the EU/EEA some of the highest rates of anti-HCV are detected among prison populations (4.3–86.3%). Further, 21.9% of the included prison populations were PWID demonstrating the intertwined relationship between at risk-groups. However, recent data are missing.
This paper aimed to describe the prevalence among GP and at-risk populations in Germany. This is however a simplistic approach given that populations at higher risk of VH may be exposed to several risk factors contributing to their vulnerability such as migration from a high prevalence country and sex work or prisoners who are sentenced due to IDU combined with potentially lack of access to health care services. Large-scale studies that focus on at-risk populations may determine differences in the prevalence of VH and identify frequent intersections between different at-risk groups in order to identify sub-populations in particular need of intensified testing and treatment efforts.
Some at-risk populations were missing in the identified literature including sex workers, persons with frequently changing sex partners, recipients of blood transfusions, and persons with tattoos and piercings. This indicates a need for more research to generate valid estimates of the prevalence in these groups to know the true burden of VH in Germany.
Methodology—Strengths and Limitations
The broad search string used in this overall scoping review ensured that all relevant outcomes were included and reviewed. By running the search string also in CC Med Base Bielefeld, it was ensured that evidence published only in German was included. Almost half of the identified publications in the “prevalence” category were published in German (24 of 51 publications), which highlights the need to search for publications in both German and English to gain insights into ongoing research and results from Germany.
The quality of the evidence was overall good with risk of bias being low in the majority of the included publications. We used the tool developed by Hoy et al. (8) specifically developed with the purpose of assessing risk of bias in prevalence studies with the focus on looking at the attempt made by the studies in minimizing the risk of bias. The majority of the studies were not population-based prevalence surveys aiming to estimate the national prevalence of HBV, HCV, or HDV, but rather studies with non-probability based sampling methods and small sample sizes. Therefore, they failed to address some of the critical items necessary to reduce bias as set forth in the risk assessment tool by Hoy et al. Although the results were not analyzed based on the risk of bias, this was an important step in order to allow critical interpretation of data and be aware of their strengths and limitations.
Our scoping review has limitations. There is a risk of publication bias and delays in the available and published data. Attempts were made to compensate this by including non-published articles from the RKI Epidemiological Bulletin (EpiBull) and relevant regional journals. Moreover, a manual search was performed of reference lists in the included publications of the overall scoping review on VH epidemiology (6), and 14 references were identified but none were on prevalence. Further efforts, such as conducting a search for gray literature through other sources would potentially increase the number of relevant non-published literature.
This analysis was part of a large comprehensive review covering all aspects of viral hepatitis B, D, and D epidemiology in Germany and presents data on VH prevalence until 2017 (6). With this comprehensive review, information on the baseline situation which is necessary for better monitoring of VH elimination in Germany was collected. The time period before 2017 is of special interest as it serves as baseline to identify where the evidence gaps are and where the prevalence data are missing. An update of the overall review, including prevalence data, is planned to be conducted within the next few years, where the current review will serve as baseline.
Comparisons between the publications in this analysis are challenging because of their heterogeneity. The publications have made use of different study design, population, age-groups, and marker etc. which hinders the drawing of conclusions on patterns and temporal trends of prevalence. Similarly, geographical trends were not possible to analyse due to too few publications with same methodology from the same regional areas in Germany.
Publications with self-reported data and data where the diagnostic marker was not specified were included in this review. However, it is important to emphasize that these cannot be compared to studies based on laboratory data and data with specific diagnostic markers. Therefore, they are mentioned in the text and Table 2 as our aim with the review was also to outlay where there is evidence and where there is not, but excluded from the figures as direct comparisons are not possible.
The majority of the studies included were large cross-sectional screening studies in which patients attending general practices or emergency rooms were offered screening for VH. There is a gap in evidence from longitudinal studies, which could contribute to an understanding of how the VH epidemic is evolving and would allow calculation of incidence and the effects of prevention and control measures on reaching the VH elimination targets. Differences identified in this review are more likely the results of heterogeneous methodology rather than reflections of changes in the VH epidemic. Nonetheless, blood donors represent a group for which standardized data are collected nationwide and over time. The six studies included in this review covered the period from 2005 to 2010, and throughout this 5 years' time period the HBV and HCV prevalence was low, and slightly lower in the later years [2005: 0.14% (HBV), 0.08% (HCV), 2010: 0.12% (HBV), 0.07% (HCV)].
During the time period in which the evidence identified in this review was published, the assays used to test for VH have changed. This may have contributed to a difference in prevalence found in the different studies. In particular for anti-HBc where patterns need to be carefully evaluated due to the risk of differences in sensitivity with the more recent tests having a higher sensitivity than the older tests.
It is also important to underline that some HBsAg positive may be inactive chronic carriers and thereby not sick, eligible for treatment or at risk for developing sequelae. When screening people with migrant background, in particular, many inactive HBsAg carriers with low viremia are identified. However, although not eligible for clinical treatment inactive HBsAg carriers can still transmit the virus to other persons. In this review, of the 39 publications that reported on HBV prevalence, 11 reported on either HBV DNA or HBeAg among those testing HBsAg positive. Further, screening for anti-HBc is important, as while it detects past infection, HBV can reactivate in people who are immunocompromised (e.g., PLWH).
Of the 33 publications covering HCV prevalence, only 13 tested for HCV RNA in addition to anti-HCV, which is important to demonstrate chronic HCV and replication. And importantly, our results include articles published until 2017, which means that the potential impact of the highly effective direct-acting antiviral (DAAs) treatment options on the HCV epidemic are not sufficiently covered in this review.
Conclusion
Globally, the elimination of VH is still gaining momentum. The progress of the interventions needed to reach the WHO elimination goals are being monitored (78) and the continuous need to collect strategic information to target the response is key. This review contributes to the understanding of the existing knowledge about the VH epidemic in Germany.
A comprehensive evidence-based overview of the available evidence on VH prevalence in Germany was provided. While there is overall good evidence, this is largely on HBV and HCV prevalence in the general and clinical populations. Gaps in knowledge exist for HDV and at-risk populations, and longitudinal studies are needed to uncover trends in the epidemic. Although Germany is considered a low prevalence country, high and very high rates are found among at-risk populations, in particular among PWID. Further research is needed on these groups and representative samples at the national level to gain much needed insights into the large-scale patterns of VH and the progress toward reaching the WHO elimination goals by 2030 in Germany.
Author Contributions
RZ and VB conceptualized this study. SD supervised the study. GS, NS, and SL carried out the study. GS, NS, and RZ developed the research questions and drafted the a priori protocol. GS, NS, SL, SB, RT, RZ, and SD extracted the eligible data. IS and SD performed the analyses. IS, GS, NS, MC, HW, YS, VB, RZ, and SD interpreted the results and contributed to the discussion. IS drafted the manuscript. All authors critically revised the manuscript and approved the final version.
Funding
This work was partly funded by a grant from the German Center for Infection Research (DZIF) and the German Ministry of Health (BMG). The funders did not play a role in the design and data collection, analysis, and interpretation of data or in writing the manuscript.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We thank Henriette Senst, Sarah Friedländer, and Thomas Harder for their help and inspiration in the data extraction.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpubh.2020.00424/full#supplementary-material
Supplementary S1. Filename: S1_Text_search strategy.docx.
Title of data: Search strategy.
Description of data: The search strategy used for the study to identify the included publications.
Supplementary S2. In- and exclusion criteria.
Filename: S2_Figure_In- and exclusion criteria.tiff.
Title of data: In- and exclusion criteria.
Description of data: Table listing the criteria for selection of the publications retrieved through the search.
Abbreviations
DAAs, Direct-acting antiviral treatment; BASE, Bielefeld Academic Search Engine; CC Med, Current Contents Medizin; DEGS 1, National population-based survey among adults in Germany; GP, General population; EMBASE, Excerpta Medica Database; Europe PMC, Europe PubMed Central; HCWs, Health care workers; IDU, Injecting drug use; IMIRA, Improving Health Monitoring in Migrant Populations; MEDLINE, Medical Literature Analysis and Retrieval System Online; MSM, Men who have sex with men; PICO, Participants, Interventions, Comparator, Outcome; PWID, People who inject drugs; PLWH, People living with HIV; PLWVH, populations with non-VH related underlying disease and people with VH in hepatological care; PRISMA-ScR, Preferred reporting items for systematic reviews and meta-analysis extension for scoping reviews; PROSPERO, International prospective register of systematic reviews; RKI, Robert Koch Institute; VH, Viral hepatitis.
References
2. WHO Regional Office for Europe. Action Plan for the Health Sector Response to Viral Hepatitis in the WHO European Region. Denmark: WHO Regional Office for Europe. (2016). p. 36.
3. World Health Organization. Global Health Sector Strategy on Viral Hepatitis 2016–2021. Geneva. (2016)
4. Bundesministerium für Gesundheit and Bundesministerium für wirtschaftliche Zusammenarbeit und Entwicklung Strategie zur Eindämmung von HIV Hepatitis B und C und anderen sexuell übertragbaren Infektionen. Bis 2030. - Bedarfsorientiert Integriert Sektorübergreifend. Berlin (2016).
5. Poethko-Müller C, Zimmermann R, Hamouda O, Faber M, Stark K, Ross RS, et al. Epidemiology of hepatitis A, B, and C among adults in Germany: results of the German health interview and examination survey for Adults (DEGS1). Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz. (2013) 56:707–15. doi: 10.1007/s00103-013-1673-x
6. Steffen G, Sperle I, Leendertz SA, Sarma N, Beermann S, Thamm R, et al. The epidemiology of hepatitis B, C and D in Germany: a scoping review. PLoS ONE. (2020) 15:e0229166. doi: 10.1371/journal.pone.0229166
8. Hoy D, Brooks P, Woolf A, Blyth F, March L, Bain C, et al. Assessing risk of bias in prevalence studies: modification of an existing tool and evidence of interrater agreement. J Clin Epidemiol. (2012) 65:934–9. doi: 10.1016/j.jclinepi.2011.11.014
9. Knorr B, Maul H, Schnitzler P. Prevalence of hepatitis B virus infection among women at reproductive age at a German university hospital. J Clin Virol. (2008) 42:422–4. doi: 10.1016/j.jcv.2008.03.009
10. Marcellin P, Grotzinger K, Theodore D, Demuth D, Manns M, Bañares Cañizares R, et al. Severity of liver disease among chronic hepatitis C patients: an observational study of 4594 patients in five European countries. J Gastroenterol Hepatol. (2015) 30:364–71. doi: 10.1111/jgh.12698
11. Lobstein S, Faber R, Tillmann HL. Prevalence of hepatitis B among pregnant women and its impact on pregnancy and newborn complications at a tertiary hospital in the eastern part of Germany. Digestion. (2011) 83:76–82. doi: 10.1159/000320455
12. Alba-Alejandre I, Kainer F, Friese K, Mylonas I. HBs Ag screening during pregnancy. Geburtshilfe und Frauenheilkunde. (2009) 69:537–40. doi: 10.1055/s-0029-1185750
13. Cai W, Poethko-Müller C, Hamouda O, Radun D. Hepatitis B virus infections among children and adolescents in Germany: migration background as a risk factor in a low seroprevalence population. Pediatr Infect Dis J. (2011) 30:19–24. doi: 10.1097/INF.0b013e3181ef22d5
14. Hüppe D, Zehnter E, Mauss S, Böker K, Lutz T, Racky S, et al. Epidemiology of chronic hepatitis C in Germany—an analysis of 10326 patients in hepatitis centres and outpatient units. Z Gastroenterol. (2008) 46:34–44. doi: 10.1055/s-2007-963691
15. Ernst V, P.D.m.T. Weinke, Impfverhalten internistischer Patienten; eine Studie am Klinikum Ernst von Bergmann in Potsdam; Immunisation compliance of internal medicine patients; a survey at the Hospital Ernst von Bergmann in Potsdam. Freie Universität Berlin Universitätsbibliothek, Garystr. 39, 14195 Berlin. (2012).
16. Zeiler T, Karger R, Slonka J, Ketschmer V, Radsak K. Introduction of anti-HBc testing of blood donors in Germany. Transfus Med. (2006) 16:441–3. doi: 10.1111/j.1365-3148.2006.00685.x
17. Deterding K, Heidelberger S, Wiebner B, Meining K, Cornberg M, Manns MP, et al. Knowledge and management of hepatitis B virus infected patients in Germany. Deutsche Medizinische Wochenschrift. (2012) 137:774–9. doi: 10.1055/s-0032-1304852
18. Walch L. Prävalenz und Infektiosität von Anti-HBc reaktiven Spendern bei Erst- und Mehrfachspendern im Blutspendedienst Baden-Württemberg – Hessen / vorgelegt von Lars Antonius Walch (2011).
19. Wiese M, Fischer J, Löbermann M, Göbel U, Grüngreiff K, Güthoff W, et al. Evaluation of liver disease progression in the German hepatitis C virus (1b)-contaminated anti-D cohort at 35 years after infection. Hepatology. (2014) 59:49–57. doi: 10.1002/hep.26644
20. Claus M, Kimbel R, Schöne K, Letzel S, Rose DM. Seroepidemiology of hepatitis A and B and vaccination status in staff at German schools for the handicapped. J Med Virol. (2016) 89:825–33. doi: 10.1002/jmv.24699
21. Feuchtenberger M, Schäfer A, Philipp Nigg A, Rupert Kraus M. Hepatitis b serology in patients with rheumatic diseases. Open Rheumatol J. (2016) 10:39–48. doi: 10.2174/1874312901610010039
22. Kartashev V, Döring M, Nieto L, Coletta E, Kaiser R, Sierra S, et al. New findings in HCV genotype distribution in selected West European, Russian and Israeli regions. J Clin Virol. (2016) 81:82–9. doi: 10.1016/j.jcv.2016.05.010
23. Mockenhaupt FP, Barbre KA, Jensenius M, Larsen CS, Barnett ED, Stauffer W, et al. Profile of illness in syrian refugees: A geosentinel analysis, 2013 to 2015. Eurosurveillance. (2016) 21:160. doi: 10.2807/1560-7917.ES.2016.21.10.30160
24. Hampel A, Solbach P, Cornberg M, Schmidt RE, Behrens GM, Jablonka A. Current seroprevalence, vaccination and predictive value of liver enzymes for hepatitis B among refugees in Germany. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. (2016) 59:578–83. doi: 10.1007/s00103-016-2333-8
25. Jansen K, Thamm M, Bock CT, Scheufele R, Kücherer C, Muenstermann D, et al. High prevalence and high incidence of coinfection with hepatitis B, hepatitis C, and syphilis and low rate of effective vaccination against hepatitis B in HIV-positive men who have sex with men with known date of HIV seroconversion in Germany. PLoS ONE. (2015) 10:e0142515. doi: 10.1371/journal.pone.0142515
26. Winkelmann M, Sorrentinoa N, Kleinb M, Mackea C, Mommsena P, Branda S, et al. Is there a benefit for health care workers in testing HIV, HCV and HBV in routine before elective arthroplasty? Orthop Traumatol Surg Res. (2016) 102:513–6. doi: 10.1016/j.otsr.2016.02.012
27. Wiegand J, Luz B, Mengelkamp AK, Moog R, Koscielny J, Halm-Heinrich I, et al. Autologous blood donor screening indicated a lower prevalence of viral hepatitis in East vs. West Germany: epidemiological benefit from established health resources. J Viral Hepatitis. (2009) 16:743–8. doi: 10.1111/j.1365-2893.2009.01132.x
28. Reuter S, Oette M, Wilhelm FC, Beggel B, Kaiser R, Balduin M, et al. Prevalence and characteristics of hepatitis B and C virus infections in treatment-naïve HIV-infected patients. Med Microbiol Immunol. (2011) 200:39–49. doi: 10.1007/s00430-010-0172-z
29. Wicker S, Rabenau HF, Gottschalk R, Doerr HW, Allwinn R. Seroprevalence of vaccine preventable and blood transmissible viral infections (measles, mumps, rubella, polio, HBV, HCV and HIV) in medical students. Med Microbiol Immunol. (2007) 196:145–50. doi: 10.1007/s00430-007-0036-3
30. Offergeld R, Ritter S, Hamouda O. HIV, HCV, HBV and syphilis infections among blood donors in Germany. 2005 Report from the Robert Koch Institute in accordance with Article 22 of the Transfusion Act. Bundesgesundheitsblatt - Gesundheitsforschung – Gesundheitsschutz. (2007) 50:1221–31. doi: 10.1007/s00103-007-0332-5
31. Willand L, Ritter S, Reinhard B, Offergeld R, Hamouda O. HIV, HCV, HBV and syphilis infections among blood donors in Germany 2006. Report from the Robert Koch Institute in accordance with Article 22 of the Transfusion Act. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. (2008) 51:902–14. doi: 10.1007/s00103-008-0612-8
32. Offergeld R, Ritter S, Quabeck L, Hamouda O. Epidemiological data on infections among blood donors in Germany 2007. Bundesgesundheitsblatt - Gesundheitsforschung – Gesundheitsschutz. (2010) 53:1188–96. doi: 10.1007/s00103-010-1152-6
33. Loutan L, Maitre B, Zuber P. Are medical students sufficiently vaccinated? Lab. Med. (2009) 33:223–7. doi: 10.1515/JLM.2009.035
34. Müller MC, Pichler M, Martin G, Plörer D, Winter C, Pogarell O, et al. Burden of disease and level of patient's medical care in substitution treatment for opiates. Med Klin. (2009) 104:913–7. doi: 10.1007/s00063-009-1196-2
35. Offergeld R, Ritter S, Hamouda O. HIV HCV, HBV and syphilis surveillance among blood donors in Germany 2008-2010. Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz. (2012) 55:907–13. doi: 10.1007/s00103-012-1516-1
36. Baars S. Prävalenz von Hepatitis B und C bei medizinischen Fachangestellten. Arbeitsmedizin, Sozialmedizin, Umweltmedizin. (2011) 46:3.
37. Baid-Agrawal S, Schindler R, Reinke P, Staedtler A, Rimpler S, Malik B, et al. Prevalence of occult hepatitis C infection in chronic hemodialysis and kidney transplant patients. J Hepatol. (2014) 60:928–33. doi: 10.1016/j.jhep.2014.01.012
38. Schmidt CS, Schön D, Schulte B, Lüth S, Polywka S, Reimer J. Viral hepatitis in alcohol-dependent inpatients prevalence, risk factors, and treatment uptake. J Addic Med. (2013) 7:417–21. doi: 10.1097/ADM.0b013e3182a50817
39. Darstein F. Prävalenz und Risikofaktoren von Hepatitis B und C bei Patienten einer Berliner Rettungsstelle; eine analytische Querschnittstudie; Prevalence and risk factors for Hepatitis B and C among patients attending a German Emergency Department; an analytical cross-section°al study. Freie Universität Berlin Universitätsbibliothek, Garystr. 39, 14195 Berlin (2015).
40. Heidrich B, Cetindere A, Beyaz M, Stahmeyer JT, Basaran MM, Braynis B, et al. High prevalence of hepatitis markers in immigrant populations: a prospective screening approach in a real-world setting. Eur J Gastroenterol Hepatol. (2014) 26:1090–7. doi: 10.1097/MEG.0000000000000164
41. Mone JS. Untersuchungen zur nicht bestimmungsgemäßen Verwendung von Substitutionsmitteln und zum Gesundheitszustand von substituierten und nicht substituierten Opiatabhängigen in Deutschland. Medizin: Universität Hamburg (2015).
42. Kant J, Kratzsch J, Maier M, Liebert UG, Berg T, Wiegand J. HBsAg and anti-HCV screening in elderly hospitalized patients of a German tertiary referral centre. Zeitschrift fur Gastroenterologie. (2016) 54:231–7. doi: 10.1055/s-0041-106656
43. Bremer V, Cai W, Gassowski M, Haußig J, Marcus U, Nielsen S, et al. Drogen und chronische Infektionskrankheiten in Deutschland - DRUCK-Studie; Infektions- und Verhaltenssurvey bei injizierenden Drogengebrauchenden in Deutschland - Abschlussbericht: 01.02.2016. Robert Koch-Institut (2016).
44. Wolffram I, Petroff D, Bätz O, Jedrysiak K, Kramer J, Tenckhoff H, et al. Prevalence of elevated ALT values, HBsAg, and anti-HCV in the primary care setting and evaluation of guideline defined hepatitis risk scenarios. J Hepatol. (2015) 62:1256–64. doi: 10.1016/j.jhep.2015.01.011
45. Wicker S, Rabenau HF, Scheller B, Marzi I, Wutzler S. Prevalence of blood-borne pathogens among 275 trauma patients: a prospective observational study. Unfallchirurg. (2016) 119:648–53. doi: 10.1007/s00113-015-0110-z
46. Bert F, Rindermann A, Abdelfattah MA, Stahmeyer JT, Rossol S. High prevalence of chronic hepatitis B and C virus infection in a population of a German metropolitan area: a prospective survey including 10 215 patients of an interdisciplinary emergency unit. Eur J Gastroenterol Hepatol. (2016) 28:1246–52. doi: 10.1097/MEG.0000000000000702
47. Schmidt AJ, Marcus U. Self-reported history of sexually transmissible infections (STIs) and STI-related utilization of the German health care system by men who have sex with men: Data from a large convenience sample. BMC Infect Dis. (2011) 11:132. doi: 10.1186/1471-2334-11-132
48. Schulte B, Stöver H, Thane K, Schreiter C, Gansefort D, Reimer J. Substitution treatment and HCV/HIV-infection in a sample of 31 German prisons for sentenced inmates. Int J Prison Health. (2009) 5:39–44. doi: 10.1080/17449200802692144
49. Tomeczkowski J, Cornberg M. Hepatitis C in Deutschland: Eine Analyse von Daten gesetzlicher Krankenkassen. Dtsch Med Wochenschr. (2015) 140:7. doi: 10.1055/s-0041-101101
50. Thonnes S, Friedel H, Frohlich H. The number of patients with chronic hepatitis C in times of new therapy options: a retrospective observational study on German health insurance funds data. Eur J Gastroenterol Hepatol. (2017) 29:503–8. doi: 10.1097/MEG.0000000000000819
51. Vermehren J, Schlosser B, Domke D, Elanjimattom S, Müller C, Hintereder G, et al. High prevalence of anti-HCV antibodies in two metropolitan emergency departments in Germany: a prospective screening analysis of 28,809 patients. PLoS ONE. (2012) 7:e41206. doi: 10.1371/journal.pone.0041206
52. Dogiami B. Estimation of the occurence of hepatitis C in the Ruhr Area [Evaluation der Inzidenz der Hepatitis C im Ballungsraum Ruhrgebiet], in Med Faculty of Hohen. Ruhr-Universität Bochum. (2014).
53. daCosta DiBonaventura M, Yuan Y, Wagner JS, Italien GJ, Lescrauwaet B, Launglay P, et al. The burden of viral hepatitis C in Europe: a propensity analysis of patient outcomes. Eur J Gastroenterol Hepatol. (2012) 24:869–77. doi: 10.1097/MEG.0b013e3283551dee
54. Jablonka A, Solbach P, Ringe B, Schleenvoigt BT, Dopfer C, Hampel A. Niedrige seroprävalenz von hepatitis C bei flüchtlingen in Deutschland im Jahr 2015. Notfall. (2017) 20:1–5. doi: 10.1007/s10049-016-0198-x
55. Magistro M. Versorgungssituation von Patienten mit chronischer Hepatitis B an der Universitätsklinik Ulm von 2000–2008. Universität Ulm. Medizinische Fakultät (2014).
56. Erhardt A. Retrospective analysis of chronic HDV in a West German university clinic over two decades: migratory pattern, prevalence and clinical outcome. Zeitschrift fur Gastroenterologie. (2010) 48:813–7. doi: 10.1055/s-0028-1109984
57. Reinheimer C, Doerr HW, Berger A. Hepatitis delta: on soft paws across Germany. Infection. (2012) 40:621–5. doi: 10.1007/s15010-012-0287-9
58. Fischer C, Mauss S, Zehnter E, Bokemeyer B, Heyne R, Hüppe D. Epidemiology and clinical characteristics of patients with chronic hepatitis B (CHB) in Germany - Results of a nationwide cross-sectional study. Z Gastroenterol. (2012) 50:22–9. doi: 10.1055/s-0031-1281628
59. Hüppe D, Buggisch P, Christensen S, Heiken H, Mauss S, Naumann U, et al. Chronic hepatitis C patients prior to broad access to interferon-free treatments in Germany. Z Gastroenterol. (2016) 54:740–7. doi: 10.1055/s-0042-106731
60. Hofstraat SHI, Falla AM, Duffell EF, Hahné SJM, Amato-Gauci AJ, Veldhuijzen IK, et al. Current prevalence of chronic hepatitis B and C virus infection in the general population, blood donors and pregnant women in the EU/EEA: a systematic review. Epidemiol Infect. (2017) 145:2873–85. doi: 10.1017/S0950268817001947
61. Falla AM, Hofstraat SHI, Duffell E, Hahné SJM, Tavoschi L, Veldhuijzen IK. Hepatitis B/C in the countries of the EU/EEA: a systematic review of the prevalence among at-risk groups. BMC Infect Dis. (2018) 18:79. doi: 10.1186/s12879-018-2988-x
62. El-Serag HB. Epidemiology of viral hepatitis and hepatocellular carcinoma. Gastroenterology. (2012) 142:1264–73.e1. doi: 10.1053/j.gastro.2011.12.061
63. Aguilera A, Trastoy R, Rodríguez-Calviño J, Manso T, de Mendoza C, Soriano V. Prevalence and incidence of hepatitis delta in patients with chronic hepatitis B in Spain. Eur J Gastroenterol Hepatol. (2018) 30:1060–2. doi: 10.1097/MEG.0000000000001163
64. Hirzel C, Wandeler G, Owczarek M, Gorgievski-Hrisoho M, Dufour JF, Semmo N, et al. Molecular epidemiology of hepatitis B virus infection in Switzerland: a retrospective cohort study. BMC Infect Dis. (2015) 15:483. doi: 10.1186/s12879-015-1234-z
65. Sagnelli E, Stroffolini T, Sagnelli C, Smedile A, Morisco F, Furlan C, et al. Epidemiological and clinical scenario of chronic liver diseases in Italy: data from a multicenter nationwide survey. Dig Liver Dis. (2016) 48:1066–71. doi: 10.1016/j.dld.2016.05.014
66. Chen HY, Shen DT, Ji DZ, Han PC, Zhang WM, Ma JF, et al. Prevalence and burden of hepatitis D virus infection in the global population: a systematic review and meta-analysis. Gut. (2018) 68:316601. doi: 10.1136/gutjnl-2018-316601
68. World Health Organization Regional Office for Europe. Hepatitis C in the WHO European Region. Copenhagen: Fact sheet (2018).
69. World Health Organization Regional Office for Europe. Hepatitis B in the WHO European Region. Copenhagen (2018).
70. European Center for Disease Prevention and Control. Hepatitis B and C epidemiology in selected population groups in the EU/EEA, in Technical Report. Stockholm: ECDC (2018).
72. Santos-Hövener C, Maria S, Schmich P, Gößwald A, Rommel A, Thomas Z, et al. Improving the information base regarding the health of people with a migration background. Project description initial findings from IMIRA. J Health Monitor. (2019) 4:46–57. doi: 10.25646/5874
73. Zeisler ML, Lemcke J. Integration of migrant populations into health monitoring in Germany. Results from a feasibility study. Survey Methods. (2019) 1–11. doi: 10.13094/SMIF-2019-00013
74. Platt L, Easterbrook P, Gower E, McDonald B, Sabin K, McGowan C, et al. Prevalence and burden of HCV co-infection in people living with HIV: a global systematic review and meta-analysis. Lancet Infect Dis. (2016) 16:797–808. doi: 10.1016/S1473-3099(15)00485-5
75. Vaux S, Chevaliez S, Saboni L, Sauvage C, Sommen C, Barin F, et al. Prevalence of hepatitis C infection, screening and associated factors among men who have sex with men attending gay venues: a cross-sectional survey (PREVAGAY), France, 2015. BMC Infect Dis. (2019) 19:315. doi: 10.1186/s12879-019-3945-z
76. European Center for Disease Prevention and Control. Hepatitis C Annual Epidemiological Report for 2017. Surveillance Report. (2017) Stockholm: ECDC.
77. Trickey A, Fraser H, Lim AG, Peacock A, Colledge S, Walker JG, et al. The contribution of injection drug use to hepatitis C virus transmission globally, regionally, and at country level: a modelling study. Lancet Gastroenterol Hepatol. (2019) 4:435–44. doi: 10.1016/S2468-1253(19)30085-8
Keywords: hepatitis B, hepatitis C, hepatitis D, scoping review, epidemiology, prevalence
Citation: Sperle I, Steffen G, Leendertz SA, Sarma N, Beermann S, Thamm R, Simeonova Y, Cornberg M, Wedemeyer H, Bremer V, Zimmermann R and Dudareva S (2020) Prevalence of Hepatitis B, C, and D in Germany: Results From a Scoping Review. Front. Public Health 8:424. doi: 10.3389/fpubh.2020.00424
Received: 17 March 2020; Accepted: 14 July 2020;
Published: 28 August 2020.
Edited by:
Remco P. H. Peters, University of Pretoria, South AfricaCopyright © 2020 Sperle, Steffen, Leendertz, Sarma, Beermann, Thamm, Simeonova, Cornberg, Wedemeyer, Bremer, Zimmermann and Dudareva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ida Sperle, c3BlcmxlLWhldXBlbGkmI3gwMDA0MDtya2kuZGU=