Jianyang Liu
Sherif M. Sherif- Alson H. Smith Jr. Agricultural Research and Extension Center, School of Plant and Environmental Sciences, Virginia Tech, Winchester, VA, United States
The sustainable fruit production in temperate and boreal regions is often imperiled by spring frosts. The risk of frost damage and the resulting economic losses have been increasing in the recent years as a result of the global climate change. Among the many approaches in mitigating frost damages, an ethylene-based compound, ethephon has proven to be effective in delaying bloom time in many fruit species and, thereby, avoid frost damage. However, effective concentrations of ethephon are often associated with harmful effects on fruit trees, which largely limit its use. Relatively, limited research attention has been given to understand the mechanisms underlying this ethylene-mediated bloom delay, thus hindering the progress in exploring its potential in frost protection. Recent advances in omics and bioinformatics have facilitated the identification of critical molecular and biochemical pathways that govern the progression of bud dormancy in deciduous woody perennials. In this review, we summarized our current understanding of the function of ethylene and its interaction with other networks in modulating dormancy and blooming in temperate fruit trees. Some possible mechanisms are also proposed that might potentially guide future studies attempting to decipher the dormancy regulation or searching for methods to alleviate frost damages.
Introduction
Fruit production in temperate and boreal regions is often threatened by spring frosts. Deciduous trees lose their hardiness to cold temperature after bud break in response to rising temperatures in the spring, and frost damage may occur if the temperature drops below or near freezing again. Trees can suffer from two types of frosts: advection and radiation, with the former resulting from large cold air mass intruding an area, and the latter due to the rapid heat loss to the atmosphere, which typically occurs on calm and clear nights. In the context of global climate change, the risks of frost damage and the resultant economic losses on the global scale have been increasing at a steady pace (Augspurger, 2013). In this regard, it becomes increasingly imperative to develop technically sound and economically feasible strategies that can effectively alleviate frost damage in today’s fruit industry.
The existing methods in protecting against frost can be classified into active and passive types. Active approaches are ad hoc and can exhibit immediate and direct effects. Such methods usually involve the use of wind machines and helicopters to downdraft the warmer air aloft in the inversion layer 10–50 m above ground, surface irrigation by sprinklers to take advantage of the fusion heat in the water, and heaters (e.g. solid fuel, propane) to prevent frost formation. These approaches mitigate, instead of avoiding frost damage, and their efficacy is highly dependent on external factors such as frost types, wind speed, dew point, wet-bulb temperature, etc. (Tsipouridis et al., 2006; Unterberger et al., 2018). In addition, active methods are generally labor-intensive, cost-ineffective, and environmentally unsustainable. In contrast, passive methods are pre-emptive and have relatively long-lasting effects. These include breeding and selection of cold-hardy and/or late-bloom varieties, selection of proper plantation site (e.g. to avoid frost pockets), and treatment with plant growth regulators (PGR) to increase freezing tolerance (Durner, 1995; Nzokou and Paligwende, 2008), to extend the duration of bud dormancy (Durner and Gianfagna, 1991; Seeley et al., 1992), or to delay flowering time (Moghadam and Mokhtarian, 2006; Grijalva-Contreras et al., 2011). In particular, the application of PGR holds great potential in frost protection due to their high efficiency, low cost, and ease of implementation.
Endodormancy of buds is an adaptative mechanism that temperate species have evolved to survive the adverse conditions during wintertime. During endodormancy, plant regrowth is repressed by intrinsic signals and remain unresponsive to environmental cues (Rohde and Bhalerao, 2007). In this review, endodormancy will be used interchangeably with dormancy, unless specified otherwise. After entering dormancy, plants track the number of chilling hours (0–7.2 °C) and exit dormancy only when a certain number of chilling hours, also known as chilling requirement (CR), is satisfied. Chilling requirements are tightly controlled by genetics and are highly variable across species and varieties. Upon completion of dormancy, resumption of plant growth is inhibited by unfavorable environmental conditions, rather than internal cues, and this period is termed as ecodormancy (Lang, 1987). To reach full bloom stage, deciduous fruit species need to satisfy heat requirements (HRs), a period of warm temperature, which is also a genotype-dependent trait (Fan et al., 2010). Bud dormancy and regrowth cycle in deciduous woody perennials are genetically programmed, highly regulated, as well as subject to the influence of many internal and external factors.
Recent studies have also indicated that global climate change exerts profound impacts on the phenology of dormancy and flowering and the increasing risk of spring frost. Alterations in plant phenology, such as shortened dormancy and early budburst and flowering, have been observed in many woody perennials in the Northern Hemisphere (Augspurger, 2013; Ma et al., 2019). For example, apple blooming in Europe has advanced for a total of 6–9 days over the last 30 years (Vitasse et al., 2011; Hoffmann and Rath, 2013; Vitasse et al., 2018), and this trend is projected to continue for decades ahead (Unterberger et al., 2018). Combined with other consequences of the climate change such as the number of frost days during the growing season (Liu et al., 2018) and the frequency of the warm spells in the late winter or early spring (Ma et al., 2019), all these factors contribute to increasing the odds of frost damage.
Ethylene Control of Bud Dormancy
Ethylene is a gaseous plant hormone that plays important roles in a plethora of physiological aspects, especially in responses to environmental stresses and biotic attacks (Bleecker and Kende, 2000). Ethylene is derived from methionine, which undergoes a series of conversions catalyzed sequentially by S-adenosyl methionine (SAM) synthetase, aminocyclopropane-1-carboxylic acid (ACC) synthase, and ACC oxidase (Wang et al., 2002). The conversion of SAM to ACC is a rate-limiting step, and ACC has been recognized to have similar functions as ethylene in mediating plant development and defense responses (Nascimento et al., 2018). In the ethylene signaling pathway, the endoplasmic reticulum (ER)-located ethylene receptors (ETR), upon activation by ethylene, releases Ethylene Insensitive-2 (EIN2), a positive regulator of ethylene signaling, from the repression by the protein kinase Constitutive Triple Response 1 (CTR1). Activated EIN2 migrates to nucleus to activate the transcription factors ethylene insensitive like (EIN3/EILs), which in turn induces the expression of a large multigene family of ethylene response factors (ERFs) (Merchante et al., 2013). Both ethylene biosynthesis and signaling have been found to mediate in the regulation of dormancy, as application of ethylene antagonist 2, 5-norbornadiene (NBD) accelerates dormancy break in potato micro-tuber, (Suttle, 1998), and mutation with impaired ethylene reception prevents the initiation of dormancy in chrysanthemum (Chrysanthemum morifolium), even after treatment with high dosage of ethephon (Sumitomo et al., 2008). The finding that many genes associated with ethylene biosynthesis and signaling (e.g. ETR2, EIN3, EIN4, and ERFs) in poplar are upregulated in dormancy-inducing conditions (Ruttink et al., 2007) also reinforces the notion that ethylene plays an essential role in dormancy induction.
Ethylene as a Bloom-Delaying Agent
Ethylene has been used to delay bloom and avoid frost damage on fruit trees for decades. Ethephon is a plant growth regulator that degrades and releases ethylene once entering plant cytoplasm (Pahwa and Ghai, 2015). Many studies have reported that fall application of ethephon can effectively delay the blooming time in the following spring in many fruit species, especially stone fruits (Table 1). According to these studies, ethephon-induced bloom delay can range from 3 up to 18 days, depending on the concentrations and application time. In general, early application with higher concentration is more effective. For example, when applied at 10% leaf drop, 250 and 500 ppm ethephon delayed bloom in plums (Prunus domestica) by 13 and 16 days, respectively; whereas only 5 and 7 days bloom delay was observed when ethephon was applied at 50% leaf drop stage (Crisosto et al., 1990). The effectiveness of ethephon appears to be limited to the pre-dormancy stage, as serious flower bud abscission was induced when application was made after the satisfaction of CR (Durner and Gianfagna, 1991), and little or no effect was found when ethephon was applied in apricot during the dormancy stage (Grijalva-Contreras et al., 2011). In addition to delaying bloom date, fall application of ethephon was also found to enhance cold hardiness of dormant buds. In peach, a frost of -3.3°C during the bud expansion period resulted in a loss of 34% in the untreated buds, but only 8% mortality was recorded in the ethephon treated buds. Moreover, 30–40% of the buds treated with ethephon survived a -23°C mid-winter low temperature, whereas only 10% of untreated buds remained viable (Gianfagna et al., 1989). Similarly, Durner (1995) showed that ethephon treated peach flower buds were 0.8–2.8°C hardier than nontreated buds during mid-December through March in the northern East Coast. Practically, the consequences of delaying bloom and improving cold hardiness are significant, as lessening the effects of winter injury and spring frost damage can greatly contribute to the increase of crop yields.
Table 1 Effects of Autumn-Applied Ethephon on Fruit Trees.
Limitations of Using Ethylene in Delaying Bloom
Despite the significant success in using ethephon to delay bloom and prevent spring frost, some studies have indicated beneficial effects of ethephon can be comprised by the occurrence of detrimental effects such as gummosis, leaf yellowing and abscission, terminal dieback, flower abscission, floral bud failure, low fruit set, and yield reduction (Table 1). Furthermore, fall application of 75–300 ppm ethephon on almond caused up to 3-fold yield reduction, and the yield reduction was in proportion to the ethephon concentrations (Grijalva-Contreras et al., 2011). Such linear yield decrease caused by increasing ethephon concentrations was also documented in peach (Crisosto et al., 1990). The formation and exudation of gums on the trunk or limbs of fruit trees, known as gummosis, is another major problem that pesters fruit growers when using ethephon. Tree gums are mainly composed of polysaccharides and are induced by various environmental stresses, mechanical or chemical injury, insect attack, or infection (Saniewski et al., 2006). Ethylene has been implicated as the leading factor that induces gummosis (Li et al., 2014). Ethylene can act synergistically with jasmonic acid to cause the breakdown of cell membranes and cell disintegration, which is the first step of the gum formation (Saniewski et al., 2006). Application of ethephon can induce gummosis in the bulbs of grape hyacinth (Muscari armeniacum) within several days (Miyamoto et al., 2010). Ethephon-induced gummosis in fruit trees, especially of stone fruit species, has been reported in many studies (Moghadam and Mokhtarian, 2006; Miyamoto et al., 2010; Miyamoto et al., 2019). Though literature lacks the data on the yield reduction due to ethephon induced gummosis, growth retardation and value loss caused by gummosis in fruit trees can be substantial (Beckman, 2003; Ezra et al., 2017). As ethephon-based PGRs are widely used in today’s fruit production practices, none of them are explicitly labeled as generic bloom-delaying agents for fruit trees in general. Recently, ethephon has been suggested to be potentially hepatotoxic (Bhadoria et al., 2018), and this may further limit the scope of its use.
Possible Mechanisms by Which Ethylene Delays Bloom
Limited information is available on how the effect of a fall application of ethephon is carried over to influence the floral behavior during the following spring. The time of full bloom depends on the fulfillment of both chilling and HR; the former dictates endodormancy break and the latter, ecodormancy release (Campoy et al., 2012). Accordingly, the delayed bloom may result from an inadequate accumulation of chilling or heat. Indeed, previous studies have indicated that chilling accumulation negatively correlates with the number of days to full bloom and the HR (Durner and Gianfagna, 1991; Li et al., 2016). Durner and Gianfagna (1991) proposed that ethephon can reduce the effectiveness of chilling in breaking flower bud dormancy, as ethephon treated flower buds needed about 3 additional weeks of chilling exposure to reach the CR compared to controls. By examining excised peach branches, Coston et al. (1985) found that ethephon-treated flower buds grew slower than untreated buds in controlled conditions, even after the fulfillment of CR. Some early studies also supported this finding in which ethephon leads to altered bud responses to warm temperatures after endodormancy release, manifested as delay in the differentiation of flower buds and the growth rate (Crisosto, 1989; Gianfagna et al., 1989). These findings indicate that ethephon may prolong both endo- and eco-dormancy duration, and influence the springtime phenology of floral buds, which appear to develop slower responsiveness to seasonal changes. Particularly, if the ethephon increases the CR to the point that exceeds the local chilling hours, significant bloom delay will likely occur. Though many studies have gained some insights into the physiology of fruit trees influenced by ethephon, none of them have explored the underlying mechanisms of how ethylene affects bud ontogeny during endodormancy and their growth after dormancy break, especially at the molecular level, and more critical studies are still needed to answer these questions.
The Function of Ethylene in Dormancy Induction May Be Also Attributed to Its Interaction With Other Hormonal Pathways Such as ABA and GA, the Two Key Hormones That Act Antagonistically in Controlling the Establishment and Release of Bud Dormancy. It Has Been Observed That Ethylene Induces the Accumulation of ABA in Many Natural Physiological Processes (Grossmann and Hansen, 2001), Presumably Through Upregulating Genes Involved in the ABA Biosynthesis (Rodrigo et al., 2006). Indeed, the Ethylene Precursor, ACC, Was Found to Activate ABA Signaling Pathway in Leafy Spurge (Euphorbia Esula) (Dogramaci et al., 2013). the Critical Role of Ethylene in Dormancy Induction Has Become More Evident When an Ethylene-Insensitive Mutation (Etr1) in White Birch (Betula Pendula) Diminished ABA Accumulation and Delayed Dormancy Initiation (Ruonala et al., 2006). on the Other Hand, Studies in Arabidopsis Have Shown That Ethylene Signaling Components (I.E. CTR1, Erfs) Can Interfere With GA Signaling by Stabilizing DELLA Proteins, the Key Negative Regulator of GA Response, Leading to Growth Inhibition (Dubois et al., 2013; Dubois et al., 2015) and Bloom Delay (Achard et al., 2007). in Tobacco (Nicotiana Tabacum), Ethylene Was Also Found to Mediate in the Light (Low R:FR) Signal Transduction and GA Acts Downstream of Ethylene Signaling (Pierik et al., 2004). a More Detailed and Graphical Explanation of Ethylene Interactions With Other Pathways That Are Associated With Dormancy Induction Was Summarized in a Recent Review by Liu and Sherif (2019).
Considering the essential role of ethylene in plant defense and stress signaling pathways, the ethylene-induced bloom delay may be a result of the activated or amplified stress responses. If exogenous ethylene is sensed by plants as a stress signal, longer dormancy, and late flowering would be advantageous for perennial plants to survive the unfavorable conditions, since in terms of fitness, survival outweighs reproductive success in perennials (Shefferson, 2009). Some evidence has emerged to support this hypothesis. The aforementioned gummosis evidently links ethylene to plant defense network, as gummosis has a strict connection to defense response against biotic attacks (Saniewski et al., 2006). Moreover, a study in Arabidopsis showed that treatment of ethylene precursor ACC rapidly decreases cell proliferation rates by arresting the cell cycle, in a similar way as osmotic stress (Skirycz et al., 2011). Such ACC-induced arrest of cell cycle was shown to cause dwarfism in leafy spurge that mimics the dormant phenotype (Dogramaci et al., 2013). The high resemblance between stress and dormancy with regard to cell cycle arrest and growth inhibition suggests that they may share common or overlapping networks acting downstream of ethylene signaling pathway. Growth arrest proceeds, and is a prerequisite for the dormancy induction, and accelerated growth cessation has been suggested to be associated with greater dormancy depth and late bud burst (Kalcsits et al., 2009). In light of this, early growth arrest induced by exogenous ethylene may be responsible for the extended dormancy and bloom delay. Nevertheless, how precocious growth cessation intensifies dormancy and delays bud burst still needs further examination.
In a recent review, Beauvieux et al. (2018) highlighted the role of reactive oxygen species (ROS) as an important signal in mediating the progression of dormancy. The findings that ethylene enhances the production of ROS in response to stress (Zhang et al., 2016) and the ROS levels increase during the ethylene biosynthesis (Ionescu et al., 2017) point to the dual role of ethylene in: a) establishing basal level of ROS at the dormancy induction, and b) activating ROS detoxication mechanism to release dormancy by triggering transient ROS increase. Thus, if the fall-applied ethephon causes the ROS levels to be higher than what would be induced by natural environmental cues, such as short photoperiod (Karpinski et al., 2003) or cold temperature (Heidarvand and Amiri, 2010), intensified or extended dormancy will likely occur. It has been shown that stress can induce post-translational modifications. Although some modifications can be reset back to the basal level upon the relief of the stress, some can be carried forward as “stress memory” to exert long term effects (Chinnusamy and Zhu, 2009). Increasing evidence has indicated that histone acetylation is necessary for the transcriptional regulation of ethylene signal transduction elements, i.e. EIN3 and EIL1 (Wang et al., 2017; Zhang et al., 2017; Wang and Qiao, 2019). Therefore, if such ethylene mediated chromatin modifications are retained, they may likely affect the subsequent events of dormancy and flowering.
Concluding Remarks and Future Perspectives
The phenology of plant dormancy and flowering are of great agricultural and economic importance. Ethylene exerts strong control over the progression of dormancy and flowering, and elucidation of its regulation mechanisms, especially its interaction and crosstalk with other signaling networks holds the key to formulate effective approaches for frost mitigation and avoidance. This review intended to summarize the current knowledge of ethephon-mediated bloom delay in temperate fruit trees and highlight the possible mechanisms by which ethylene interferes with the initiation, maintenance, and release of bud dormancy. As ethylene is at the hub of crossroads to defense- and stress-related signaling pathways, it might be meaningful to examine ethylene in the context of stress responses. Emerging evidence has suggested that pathways of stress response and dormancy may converge at the stages of growth cessation, and bud break. Nonetheless, specific questions that still need to be addressed may include: 1) how ethylene affects chilling and HRs in deciduous woody perennials; 2) how ethylene interacts with other regulatory networks such as those controlling flowering and cell cycle progression; 3) whether ethylene is modulating the level of DNA methylation and/or histone modification of dormancy-related genes, e.g. dormancy associated MADS (DAM) genes; and 4) how accelerated, rather than naturally induced, growth cessation is related to dormancy depth and budburst. Studies focusing on temporal transcriptomic and hormonal changes during the dormancy/growth cycle have indeed provided some valuable insights into the mechanisms by which ethylene mediates such multifaceted roles in bud dormancy, particularly in stone fruits. However, due to the wide range of climates under which these studies are conducted and to facilitate comparative analyses, it would be highly advisable that future investigations use chill units (CU) and growing degree hours (GDH), rather than Gregorian calendar, to time sample collections during the bud dormancy cycle.
Author Contributions
JL and SS have contributed equally to the writing, editing, and preparation of this mini-review.
Conflict of Interest
The authors declare that no conflicts of interest exist regarding the completion and publication of this research.
Acknowledgments
The authors are grateful for the funding to SS from The Virginia Agricultural Council (#449846), The Virginia Apple Research Program (#459952), and the VT's Open Access Subvention Fund.
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Keywords: spring frost, temperate fruits, deciduous woody perennials, bud dormancy, hormone signalling, ethylene
Citation: Liu J and Sherif SM (2019) Combating Spring Frost With Ethylene. Front. Plant Sci. 10:1408. doi: 10.3389/fpls.2019.01408
Received: 06 September 2019; Accepted: 10 October 2019;
Published: 30 October 2019.
Edited by:
Isabel Lara, Universitat de Lleida, SpainReviewed by:
Gilbert Neuner, University of Innsbruck, AustriaKlára Kosová, Crop Research Institute (CRI), Czechia
Copyright © 2019 Liu and Sherif. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Sherif M. Sherif, c3NoZXJpZkB2dC5lZHU=