In the original article, there was a mistake in Figure 6 as published. On the y-axis “nmol g−1 FW” was used instead of “μmol g−1 FW” for the total NPT content measurement. The corrected Figure 6 appears below.
Figure 6
A correction has been made to the Materials and Methods, subsection Quantification of Total Non-Protein Thiols:
“For the determination of total non-protein thiols (NPT), a modified method of Queval and Noctor (2007) was applied. Total thiols in leaf extract were assayed as 5,5′-dithio-bis-[2-nitrobenzoic acid]-reactive thiols (DTNB-reactive thiols) by spectrophotometry (Beckman UV- DU650, Beckman Coulter, United States) using glutathione (GSH) as a standard. Approximately 100 mg frozen leaf powder was extracted in 1 ml 0.2N HCl. Aliquots of 0.5 ml supernatant were transferred into fresh micro tubes (Sarstedt AG & Co., Nümbrecht, Germany) and neutralized with 0.4 ml 0.2 M NaOH in the presence of 50 μl 0.2 M NaH2PO4 (pH 5.6). For thiol quantification by spectrophotometry, each semi-micro cuvette (Sarstedt AG & Co., Nümbrecht, Germany) contained 500 μl phosphate-EDTA buffer (0.2 M NaH2PO4, pH 7.5; 10 mM EDTA), 50 μl of 12 mM DTNB and 450 μl neutralized sample extract (total volume 1 ml). For standards, the extract was replaced by 450 ml of 0, 10, 20, 30, 40, 50 μmol GSH. The absorbance was measured at a wavelength of 412 nm 3 min after addition of extract or standard.”
The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.
References
1
QuevalG.NoctorG. (2007). A plate reader method for the measurement of NAD, NADP, glutathione, and ascorbate in tissue extracts: application to redox profiling during Arabidopsis rosette development. Anal. Biochem.363, 58–69. 10.1016/j.ab.2007.01.005
Summary
Keywords
amino acids, Cd treatment, cysteine, glutathione, glycine, phosphorylated pathway, serine
Citation
Samuilov S, Rademacher N, Brilhaus D, Flachbart S, Arab L, Kopriva S, Weber APM, Mettler-Altmann T and Rennenberg H (2019) Corrigendum: Knock-Down of the Phosphoserine Phosphatase Gene Effects Rather N- Than S-Metabolism in Arabidopsis thaliana. Front. Plant Sci. 10:325. doi: 10.3389/fpls.2019.00325
Received
20 February 2019
Accepted
28 February 2019
Published
26 March 2019
Volume
10 - 2019
Edited and reviewed by
Jean-Philippe Reichheld, Centre National de la Recherche Scientifique (CNRS), France
Updates
Copyright
© 2019 Samuilov, Rademacher, Brilhaus, Flachbart, Arab, Kopriva, Weber, Mettler-Altmann and Rennenberg.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Sladjana Samuilov sladjana.samuilov@ctp.uni-freiburg.de
This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science
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