Response of Macrobrachium rosenbergii to Vegetable Oils Replacing Dietary Fish Oil: Insights From Antioxidant Defense

The study was conducted to evaluate the effects of fish oil replacement by vegetable oils on growth performance, histology, and antioxidant capacity of Macrobrachium rosenbergii. Three isonitrogenous and isoenergetic diets were formulated with different lipid sources included. DFO diet contained 6% fish oil, whereas DSO and DRO diets included 6% soybean oil and rapeseed oil (RO) as alternatives for fish oil, respectively. Prawns were fed thrice daily for 8 weeks. The results showed that prawns in DFO group showed significantly lower final weight, weight gain ratio, and specific growth rate (SGR), but higher feed intake and feed coefficient ratio than those in DSO and DRO groups. In hepatocellular ultrastructure, malformed and atrophic nucleus and higher apoptosis ratio were observed in DFO group. In addition, levels of haemolymph proinflammatory cytokines, activities of anti-superoxide anion, inducible-type NO-synthase (iNOS) and content of nitric oxide, and hepatopancreas NF-κB signal pathway gene expression in DFO group increased markedly compared to those of DSO and DRO groups. The results suggested that vegetable oils, such as soybean oil and RO might be the better lipid sources in diets for Macrobrachium rosenbergii than fish oil, it may be attributed to modified oxidative status induced by NF-κB-NO signal pathway.

Introduction

Marine fish oil is commonly used as the main lipid ingredient in aquafeeds, especially for rapid-growing fish and crustacean species which require LC-PUFA as EFA, such as (20:5n-3, EPA), docosahexaenoic acid (22:6n-3, DHA) and arachidonic acid (20:4n-6, AA). Providing an EFA balanced diet is not only essential for maintaining growth and food utilization, but also for immunity and resistance to oxidative stress of aquatic animals (Tocher et al., 2010).

Nevertheless, the reduction in global fish oil supply has forced this industry to use alternative fat sources in aquafeeds. The relative richness of plants makes vegetable oils a good alternative and sustainable source. Vegetable oils contain abundant C18 PUFA, such as (18:1n-9, OA), (18:2n-6, LA) and (18:3n-3, LNA), but are low in n-3 LC- PUFA (Turchini et al., 2010). Many freshwater teleosts can potentially convert LA to AA by desaturating and elongating via enzymatic pathways (Bell and Tocher, 2009), in which Δ6 and Δ5 fatty acyl desaturases (Fad) play a pivotal role. However, this capability in crustaceans is currently unknown. Previously, expression of Δ6 Fad has been conducted on euryhaline and freshwater crustaceans, Chinese mitten crab (Eriocheir sinensis) and Pacific white shrimp (Litopenaeus vannamei) (Yang et al., 2013; Chen K. et al., 2017). Δ12 and Δ15 Fads have been detected in some species of plants and invertebrates for n-3 and n-6 PUFA synthesis through LA (National Research Council [NRC], 2011). In addition, M. rosenbergii is unable to synthesize 18:2n-6 or 18:3n-3, but elongates and desaturates 18:2n-6 to 20:2n-6, 20:3n-6 and 18:3n-3 to 22:6n-3 (Reigh and Stickney, 1989). M. rosenbergii is also unable to synthesize either 20:4n-6 or 20:5n-3 from shorter chain fatty acids, but the requirements of the two fatty acids are small amount (Del Rosario González-Baró and Pollero, 1998; National Research Council [NRC], 2011). M. rosenbergii can use OA efficiently (Querijero et al., 1997). Thus, M. rosenbergii might have the capacity to use vegetable oils rich in C18 fatty acids.

Accordingly, single or blend of vegetable oils have been tested in M. rosenbergii, such as soybean oil, sunflower oil, coconut oil, palm oil, castor oil, corn oil, and RO (canola oil), and some of them (soybean oil, sunflower oil, and RO) have a comparable or better effects on growth performance compared with fish oil (Kamarudin and Roustaian, 2002; Kim et al., 2013; Kangpanich et al., 2017). Among them, soybean oil and RO have great potential to be used in the feed of M. rosenbergii because of the considerable contents of OA and LA, respectively, (National Research Council [NRC], 2011), and the abundant availability makes these two vegetable oils more preferable for low cost aquafeed production. However, some reports pointed out that vegetable oils could reduce the survival rate and post-larval production and of shrimps because of the fatty acid profiles (Petriella et al., 1984; Kamarudin and Roustaian, 2002).

Numerous studies indicate that the imbalance of dietary fatty acids can induce inflammation and oxidative stress, a reaction of immune system response to external physical, chemical stimuli or bacterial infiltration. However, chronic or excessive stress reactions could lead to inflammatory diseases. During an intracellular signal cascade, Rel/NF-κB family makes an important impact in the activation of the immunological factors involved with inflammation and apoptosis (Dixit and Mak, 2002; Hoffmann and Reichhart, 2002; Hayden and Ghosh, 2008).

There are also two NF-κB family signal pathways (Relish or Dorsal pathway) in Drosophila melanogaster, a representative invertebrate, and the two signal pathways also contribute to anti-bacterial and immune responses (De Gregorio et al., 2002; Hoffmann and Reichhart, 2002). Dorsal/Dif interacts with Cactus, an IκB-related inhibitor, but in response to the degradation of Cactus, Dorsal/Dif was activated by Toll receptor signaling and translocated to the nucleus to interact with target gene regulatory sites (Busse et al., 2007). In Drosophila, the Toll-Dorsal pathway plays a core role in response to infection by microorganisms and viruses through the synthesis of antibacterial peptides (AMPs) (Valanne et al., 2011). Similar with vertebrate p105, Relish is triggered by the IMD pathway in response to infection by activating the gene expression of AMPs in Drosophila (Lemaitre and Hoffmann, 2007). Also, some key genes in Toll and IMD pathway of M. rosenbergii have been reported, but most research focuses on viral preventing and controlling (Srisuk et al., 2014; Shi et al., 2015; Huang et al., 2016). In mammals, research has revealed that NF-κB can regulate oxidative stress by increasing iNOS activity and subsequently inducing nitric oxide (NO) synthesis (Janssen-Heininger et al., 2016; Marinello et al., 2019). NO can be combined with oxygen radicals to reduce oxidative stress (Aktan, 2004). However, less research has focused on oxidative stress regulated by dietary lipid via NF-κB/NO signal pathway in shrimp. Therefore, the signaling pathways involved in NF-κB and oxidative stress regulation in shrimp is an area for further research.

The giant freshwater prawn, M. rosenbergii, has been cultured in tropical and subtropical regions for a long time. This variety has excellent cultivation characteristics such as fast growth, high market demand, strong resistance to stress, and adaptation to euryhaline environments (Moffitt and Cajas-Cano, 2014). It supports the livelihood in low-income communities in many parts of world and strengthens the use of marginal inland waters to produce high-value goods. However, high feed cost and serious disease problem in intensive aquaculture limits the industrial development (Miao and Ge, 2002; Fu et al., 2012). Thus, the objective of the present study was to evaluate the efficacy of vegetable oils replacing dietary fish oil on growth and antioxidative capacity of M. rosenbergii.

Materials and Methods

Animal Ethics

The care and use of animals followed Animal Research Institute Committee guidelines of Nanjing Agricultural University, China. This study has been approved by the Committee of the Animal Research Institute of Nanjing Agricultural University, China [permit number: SYXK (Su) 2011-0036].

Experimental Ingredients and Diets

Three isonitrogenous (40.9% crude protein) and isoenergetic (16.3 MJ kg^–1 gross energy) diets were formulated in this study. The control diet included 6% fish oil (DFO), while fish oil was substituted with soybean oil and rapeseed oil in the test diets (DSO and DRO). Formulation and proximate composition of the experimental diets is presented in Table 1. Fish meal, casein and gelatin served as protein sources; fish oil, soybean oil, and RO were supplemented as lipid sources; α-starch and dextrin were used as carbohydrate source. Fatty acid composition of the feeds is shown in Table 2.

TABLE 1

Table 1. Formulation and proximate composition of the experimental diets.

TABLE 2

Table 2. Fatty acid composition of the feed (%).

All the ingredients were ground through a 60-mm mesh. The fine powder was carefully weighed, then 6% oil (w/w) and 30% water (w/w) were added to the mixture that was further blended to ensure homogeneity. A Laboratory pelletizer (Guangzhou Huagong Optical Mechanical & Electrical Technology CO. LTD, Guangzhou, China) was used for the pelletizing process. Feed pellets (1 mm and 1.5 mm diameter) were produced for prawns in different growth periods. After drying in feed dryer, the feeds were offered to prawns.

Shrimps and the Feeding Trial

Juvenile prawns were provided by Zhejiang Southern Taihu Lake Freshwater Aquatic Seed Industry Co., Ltd., (Huzhou, China). After 2 weeks of acclimation, prawns of similar size (0.24 ± 0.001 g) were randomly distributed into 9 concrete tanks (2.0 × 2.0 × 0.8 m) at a density of 50 prawns per tank. Three experimental diets were randomly allotted to prawns in triplicate tanks.

All prawns were fed three times daily at 7:00, 12:00, and 17:30 for 56 days, and feeding amount was 2–5% of body weight. Feces and molts were removed by siphoning. During the feeding trial, the average water temperature was 30 ± 0.4°C; continuous aeration was supplied to each tank to maintain the dissolved oxygen above 50 mg/L; pH was 7.6–8.0, and total ammonia nitrogen level was above 0.02 mg/L, Total ammonia nitrogen and nitrite were kept <0.2 and 0.005 mg/L, respectively.

Sample Collection

At the end of the feeding period, prawns were fasted for 24 h to empty the digestive tract. Before sampling, the prawns were placed in the mixture of water and ice. The hemolymph from 5 prawns was randomly sampled per tank using heparinized syringes. Hemolymph samples were collected into anticoagulation tubes, then centrifuged at 2000 × g 4°C for 10 min. Hepatopancreas samples were stored in 4% paraformaldehyde for apoptosis measurement and 2.5% glutaraldehyde for ultrastructure study, respectively. The remainder of the hepatopancreas of each prawn was stored at −80°C for subsequent analysis of gene expression.

Proximate and Fatty Acid Analysis

Diets were analyzed for proximate composition. Crude protein (nitrogen × 6.25) was determined by the Kjeldahl method using an Auto Kjeldahl System (2300; FOSS Tector, Hoganas, Sweden); crude lipid was determined by ether extraction using an Auto Soxtec System (2050; FOSS Tector); gross energy was determined by an adiabatic bomb calorimeter (PARR 1281, United States).

The total lipids of experimental diets were extracted according to the Bligh–Dyer’s method (Bligh and Dyer, 1959), and the obtained total lipids were weighed and stored at −20°C until used. Afterward, fatty acids were converted to their methyl esters for Gas Chromatographic (GC) analysis according to Nasopoulou et al. (2011). Agilent 5890 series II GC (Agilent Technologies, Santa Clara, CA, United States) was used for GC analysis of fatty acids.

Ultrastructure Study

Electron microscopy samples were fixed with 2.5% glutaraldehyde for 24 h, then fixed with 1% osmium tetroxide for 1 h and stored at 4°C. The sections were embedded in epoxy resin Epon 812, cut into70-nm-thick sections by RMC PowerTomeXL microtome, stained with uranyl acetate and lead citrate, and examined under a transmission electron microscope (Hitachi H-7650, Tokyo, Japan).

Apoptosis Detection

Hepatocyte apoptosis was determined according to Lu et al. (2013); the terminal deoxynucleotidyl TUNEL assay followed the protocol of Apoptosis Detection Kit (Nanjing Jian-Cheng Bioengineering Institute, China). The tissue sections were deparaffinized, rehydrated, and treated with protein digestion buffer at room temperature for 10–15 min, followed by incubation of biotinylated nucleotide mix with the working-strength TDT at 37°C for 60 min. The reactions were terminated by immersing the slides in 2 × saline sodium citrate at room temperature for 10–15 min. Each slide was incubated with 100 mL of dimethylaminoazobenzene (DAB) and developed until a light brown background appears. The slides were mounted in an aqueous mounting medium followed by the examination under a light microscope. The positive cell nucleus was dyed brown–yellow. The DNase1-treated tissue was used as the positive control. The reaction without TDT enzyme was used as the negative control.

Levels of Proinflammatory Cytokines

Tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), interleukin-1 (IL-1), and interleukin-6 (IL-6) in hemolymph were measured using enzyme-linked immunosorbent assay (ELISA) kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China) following the manufacturer’s protocols.

iNOS, NO and Anti-Superoxide Anion in Hemolymph

iNOS activity, NO content, and ASA activity in hemolymph was detected using a commercially available assay kit (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer’s instructions.

RNA Isolation and RT-qPCR Analysis

Total RNA was isolated using RNAiso Plus (Takara Co., Ltd., Japan), and then purified with RNase-Free DNase (Takara Co., Ltd., Japan) to avoid genomic DNA amplification. Purity and concentration of RNA were measured using a NanoDrop (DN-1000, Thermo Fisher Scientific, United States). After normalizing the concentration of the RNA samples, cDNA was generated from 500 ng DNase-treated RNA using ExScriptTM RT-PCR kit according to the manufacturer’s directions (Takara Co., Ltd., Japan).

The cDNA samples were analyzed using a real-time quantitative detector (BIO-RAD, United States) with the SYBR Green II Fluorescence Kit (Takara Co., Ltd., Japan). The fluorescent qPCR reaction solution consisted of 10 μL SYBR^® premix Ex Taq^TM, 0.4 μL ROX Reference Dye II, 0.4 μL PCR forward primer (10 μM), 0.4 μL PCR reverse primer (10 μM), 2.0 μL RT reaction (cDNA solution), and 6.8 μL dH2O. All RT-qPCR primers were designed using the Primer 5 software and listed in Table 3. The thermal profile was 95°C for 30 s, followed by 40 cycles of 95°C for 5 s and 60°C for 30 s, followed by a melt curve analysis of 15 s from 95 to 60°C, 1 min for 60°C, and then up to 95°C for 15 s. Control reactions were conducted with non-reverse transcribed RNA to determine the level of background or genomic DNA contamination. In all cases, genomic DNA contamination was negligible. β-actin was selected as the housekeeping gene to normalize our samples because of its stable expression in the present study. qPCR reactions were carried out in triplicates of each sample. Values for the threshold (CT) from the treated and control tissue templates were compared, and the 2^–△△CT method was used as the relative quantification calculation method (Livak and Schmittgen, 2001).

TABLE 3

Table 3. Sequences of the primers used in the study.

Calculations and Statistical Analysis

The growth parameters were calculated as follows, where W₀ and W_t are initial and final body weight.

$\mathrm{Weight\; gain\; rate }(\mathrm{WGR},\%)=(\mathrm{W}{}_{\mathrm{t}}-\mathrm{W}{}_0)\times 100/\mathrm{W}{}_0.$

$\mathrm{Specific\; growth\; rate }(\mathrm{SGR},\%\mathrm{day}{}^{-}{}^1)=(\mathrm{LnW}{}_{\mathrm{t}}-\mathrm{LnW}{}_0)\times 100/\mathrm{day}.$

$\mathrm{FCR\; = }\mathrm{feed}\mathrm{consumption}(\mathrm{g})/\mathrm{Weight}\mathrm{gain}(\mathrm{g}).$

$\mathrm{Relative\; feed\; intake }(\mathrm{RFI},\%/\mathrm{day})= 100\times \mathrm{total}\mathrm{amount}\mathrm{of}\mathrm{the}\mathrm{feed}\mathrm{consumed}\times 2/[(\mathrm{W}{}_0+\mathrm{W}{}_{\mathrm{t}})\times \mathrm{days}]$

Data were subjected to one-way analysis of variance (ANOVA) to investigate the growth performance, hemolymph parameters, and mRNA expression, after testing the homogeneity of variances with the Levene test. If significant (P < 0.05) differences were found, Tukey’s multiple range test was used to compare the means. Analyses were performed using the SPSS program version 16.0 (SPSS Inc., Michigan Avenue, Chicago, IL, United States) for Windows. All data were presented as means ± S.E.M (standard error of the mean).

Results

Growth Performance

As shown in Table 4, at the end of the feeding trial, final weight, WGR and SGR in DSO and DRO were significantly higher than those in DFO (P < 0.05), while no significant difference was observed between DSO and DRO (P > 0.05). Significant differences in RFI and FCR were only found between DFO and DSO (P < 0.05).

TABLE 4

Table 4. Growth performance and feed utilization of Macrobrachium rosenbergii fed with different dietary lipid sources.

Ultrastructure of Hepatocytes

As shown in Figure 1, hepatopancreas of prawns fed DSO and DRO diets showed normal ultrastructure (Figures 1b,c). In these hepatocytes, the nucleus was round and the nucleolus was visible, and the lipid droplets were big and plump. However, in DFO (Figure 1a), the nucleus was malformed and atrophic; the lipid droplets became smaller and increased in number than those in the other groups.

FIGURE 1

Figure 1. Transmission electron microscope images of Macrobrachium rosenbergii hepatocyte ultrastructure (1200 ×). (a) Prawns fed diet including 6% fish oil (DFO); (b) Prawns fed diet including 6% soybean oil (DSO); (c): Prawns fed diet including 6% rapeseed oil (DRO). N: nucleus; L: lipid droplet.

Hepatocyte Apoptosis

Apoptosis of hepatopancreatic cells was evaluated by TUNEL assay. The cells with stained brown nuclei were considered in a state of apoptosis (Figure 2) and were counted to calculate hepatopancreatic apoptosis cell ratio. Three paraffin sections were used for TUNEL assay each group. In prawns fed DFO diets, apoptotic cells were about 36% of total hepatocytes (Figure 2a). In the hepatopancreas from prawns fed DSO or DRO diets, the apoptotic cells were about 10–20% of total hepatocytes (Figures 2b,c). Thus, TUNEL positive hepatocytes of prawns fed DFO diet were significantly more than those fed DSO and DRO diets (P < 0.05) (Figure 2d).

FIGURE 2

Figure 2. Hepatocyte apoptosis of Macrobrachium rosenbergii. (a) Prawns fed diet including 6% fish oil (DFO); (b) Prawns fed diet including 6% soybean oil (DSO); (c) Prawns fed diet including 6% rapeseed oil (DRO); (d) Ratio of hepatopancreatic apoptosis cell (n = 3). Blue arrow: normal cells; yellow arrow: apoptotic cells.

Proinflammatory Cytokines in Hemolymph

The levels of inflammatory factors in hemolymph were shown in Figure 3. The levels of IL-1 and INF-γ in DSO and DRO showed significant differences compared with those in DFO (P < 0.05), but no significant differences were found between DSO and DRO (P > 0.05). The levels of IL-6 and TNF-α in DSO group showed significant difference compared with those in DFO group (P < 0.05), but there was no significant difference between the other two pairs (P > 0.05).

FIGURE 3

Figure 3. Levels of proinflammatory cytokines in hemolymph. DFO: prawns fed diet including 6% fish oil; DSO: prawns fed diet including 6% soybean oil; DRO: prawns fed diet including 6% rapeseed oil. Values are means ± SEM (n = 15). (A) IL-1 concerntration in hemolymph; (B) IL-6 concerntration in hemolymph; (C) INF-γ concerntration in hemolymph; (D) TNF-α concerntration in hemolymph. Columns with different letters indicate significant differences (P < 0.05).

Hemolymph Oxidative Status

As shown in Figure 4, the activity of iNOS and level of NO in hemolymph in DFO fed prawns were significantly higher than those in DSO and DRO fed groups (P < 0.05). ASA activity in DFO fed prawns was significantly higher than that in DSO fed group (P < 0.05), while no marked difference was observed between DFO and DRO groups (P > 0.05).

FIGURE 4

Figure 4. Hemolymph oxidative status of Macrobrachium rosenbergii. DFO: prawns fed diet including 6% fish oil; DSO: prawns fed diet including 6% soybean oil; DRO: prawns fed diet including 6% rapeseed oil; ASA: arachidonic acid; iNOS: inducible-type NO-synthas; NO: nitric oxide. Values are means ± SEM (n = 15). Columns with different letters indicate significant differences (P < 0.05).

Gene Expression in NF-κB Signal Pathway

The mRNA expression of IMD and Relish showed a similar pattern when the prawns were fed different lipid sources (Figure 5). Significant differences were found between DFO and DRO in mRNA expression of IMD (P < 0.05), while significant differences were found between DFO and DSO in Relish expression (P < 0.05).

FIGURE 5

Figure 5. Gene expression of IMD-Relish signal pathway in hepatopancreas. DFO: prawns fed diet including 6% fish oil; DSO: prawns fed diet including 6% soybean oil; DRO: prawns fed diet including 6% rapeseed oil. Values are means ± SEM (n = 15). (A) The expression of IMD in hepatopancreas; (B) the expression of Relish in hepatopancreas. Columns with different letters indicate significant differences (P < 0.05).

As shown in Figure 6, lipid sources showed significant effects on the mRNA expression of Toll, IκBα and Dorsal (P < 0.05). DFO showed the highest expression on Toll, IκBα and Dorsal, significant difference were found between DFO and DRO (P < 0.05).

FIGURE 6

Figure 6. Gene expression of Toll-Dorsal signal pathway in hepatopancreas. DFO: prawns fed diet including 6% fish oil; DSO: prawns fed diet including 6% soybean oil; DRO: prawns fed diet including 6% rapeseed oil. Values are means ± SEM (n = 15). (A) The expression of Toll in hepatopancreas; (B) the expression of IκBα in hepatopancreas; (C) the expression of Dorsal in hepatopancreas. Columns with different letters indicate significant differences (P < 0.05).

Discussion

With the increase of vegetable oil production, the prices of multifarious products in some regions have stabilized and even declined (Bell et al., 2004). Hence, in the present study, vegetable oils as candidate to replace fish oil was examined. The results showed that fish oil could be completely replaced by soybean oil or RO in giant freshwater prawn diets for an 8-week feeding period, and even these two vegetable oils showed better growth promoting effects than fish oil. Similar results were also found in some previous studies that showed the viability of replacing dietary fish oil with soybean oil completely (Piedecausa et al., 2007). In this study, the DFO diet had the highest level of PUFA, such as EPA and DHA, while the DSO and DRO diets were rich in LA and OA. The demand for dietary EFA varies widely among different species. In most freshwater fish species, the demands of EFA can be satisfied by C18 PUFA (18:3n-3, 18:2n-6), while C18 PUFA cannot be used to synthetize the EFA in marine fish (Tocher et al., 2010). According to National Research Council [NRC] (2011), the EFA requirements of M. rosenbergii are 0.075% (DHA) and 0.08% (AA). According to the dietary fatty acid composition in the present study, all the experimental diets included with 22% fish meal could satisfy the demands of the LC-PUFA. Growth inhibition in DFO group might be attributed to the dietary excess n-3 LC-PUFA (1.10% EPA, 0.35% DHA) rather than optimum demands of M. rosenbergii. Similar results on fish oil replacement by vegetable oils in M. rosenbergii also showed that vegetable oils had a better effect on growth improvement than fish oil when the basal diet included 9.8–35% fish meal (Kim et al., 2013; Kangpanich et al., 2017). Inverse result was observed when no fish meal was incorporated in basal diet (Muralisankar et al., 2014). Thus, 10% dietary fish meal could supply enough amount of LC-PUFA for M. rosenbergii. It was supported by the data that showed excess n-3 HUFA can cause poor growth performance, hepatic pathology, and blood lipid peroxidation in grass carp (Ctenopharyngodon idella) (Du et al., 2010), growth restriction and high mortalities in Russian sturgeon (Acipenser gueldenstaedtii) (Zhu et al., 2017), and high mortalities and muscular lesions in European sea bass (Dicentrarchus labrax) (Betancor et al., 2011). It was further supported by the fact that only vegetable oil or mix of vegetable oil and fish oil was incorporated in the commercial diet included with fish meal of M. rosenbergii, but the ratio of fish oil decreased with the increasing proportion of dietary fish meal (Tidwell et al., 1993; Radhakrishnan et al., 2014; Banerjee and Khemundu, 2019).

The histological examination is considered as the gold standard of liver damage detection. Compared with DFO, significant alterations in the ultrastructure of hepatopancreas were found in DSO and DRO, in which lipid droplets were plump and bigger, as well as the nucleus was clear. The atrophy of the organelles in DFO group might be atributted to the excess LC-PUFA in fish oil. Previous studies had evaluated that excess dietary unsaturated fatty acids could cause functional damage to cells through lipid peroxidation (Matés et al., 1999), which could increase levels of reactive oxygen species, attenuate the body antioxidant defenses, and introduce liver injury (Atalay et al., 2000; Sanders et al., 2004; Nieto, 2007). The oxidant status is the decisive factor of most kinds of steatosis hepatis (Tariq et al., 2014). Additionally, many studies indicate that higher dietary soybean oil levels could induce lipid deposition in the liver of fish (Li et al., 2016), which is similar with the result of the present study. This conclusion is further supported by the studies that show dietary LA can increase lipid deposition both in the livers of mammals and fish (Pérez-Matute et al., 2007; Muhlhausler and Ailhaud, 2013). This may be attributed to vegetable oils that increase the key enzyme activies associated with fatty acid synthesis.

Moreover, hepatocyte apoptosis is often associated with hepatopancreas lipid peroxidation, and the evaluation of apoptosis is considered as a novel biomarker of disease severity of liver in human (Wieckowska et al., 2006). In this study, DFO-fed prawns were significantly TUNEL-positive compared to those in the other groups. The steatotic hepatocyte appears to be more susceptible to apoptosis. A previous study reported that fish oil can modulate the redox environment by increasing apoptosis, which is mediated by ROS (Sanders et al., 2004), but other reports concluded fish oil has the ameliorating effects of oxidative stress and apoptosis (Myhrstad et al., 2014; Hong et al., 2015). The contradiction might be due to the dose because excess n-3 HUFA led to lipid peroxidation and oxidative stress in the present study.

Cytokines including the interferons, the interleukins, and TNF-α were traditionally considered to be unique to vertebrates. However, functional analogs of cytokines, such as IL-1, IL-2, IL-6, and TNF-α have been identified in different groups of invertebrates (e.g. annelids, tunicates, molluscs, echinoderms) (Ottaviani et al., 1996). These invertebrate-derived cytokine-like molecules have been shown to share many biological activities with vertebrate equivalents because of their conserved structure (Nappi and Ottaviani, 2000). In addition, a growing number of cytokines of shrimps have been identified in recent years. The TNF members have been identified in Marsupenaeus japonicus, Litopenaeus vannamei, and Macrobrachium nipponense (Mekata et al., 2010; Wang et al., 2012; Qin et al., 2019). The interleukin-1 receptor associated kinase-1 and interleukin-16-like gene were characterized in Fenneropenaeus penicillatus and Litopenaeus vannamei (Liang et al., 2017; Xu et al., 2017). The interferon regulatory factor was also identified in Litopenaeus vannamei (Li et al., 2015). The above studies might support indirectly that IL-1, IL-6, INF-γ, and TNF-α are also found in M. rosenbergii, and these cytokines could be good indicators to assess the effect of lipid sources on shrimp health as they are the main effectors of immune responses.

In the present study, contents of TNF-α, INF-γ, IL-1, and IL-6 significantly decreased in DSO compared with that in DFO, the same as IL-6 and TNF-α in DRO. It is postulated that proinflammatory cytokines production could be induced by oxidative stress which was caused by fish oil (Naik and Dixit, 2011), and the lipid peroxidation could be the main reason of the oxidative stress. Similar results were also found in common carp (Cyprinus carpio) (Chen X. M. et al., 2017), large yellow croaker (Larmichthys crocea) (Li et al., 2013), and rat (Rattus norvegicus) (Sanders et al., 2004). Additionally, considerable research supports that n-3 PUFA can induce lipid peroxidation (Pupe et al., 2002; Muralidhar et al., 2004). However, numerous studies have clearly described that n-3 LC-PUFA reduced the expression of inflammation-related genes in vertebrate macrophages, which caused a decreased proinflammatory cytokines production (Weldon et al., 2007; Mullen et al., 2010). However, little literature is available on shrimp, and the contradiction might be due to dietary levels of n-3 LC-PUFA. According to previous research, the anti-inflammatory effects induced by n-3 LC-PUFAs depend on the stimulation of macrophages, while macrophages can be induced by an appropriate type and dose of stimulants (Mullen et al., 2010; Forlenza et al., 2011). Therefore, the unconspicuous anti-inflammatory effects of n-3 LC-PUFAs in the present study could be due to the short of stimulating start-up process.

In the present study, prawns fed vegetable oils showed significantly lower iNOS and ASA activities, as well as NO contents than those fed fish oil. NO might behave as a potential antioxidant agent relying on its ability to eliminate oxygen free radicals. Previous in vitro studies support this concept in as much as NO is able to inhibit lipid peroxidation (Violi et al., 1999). Similar results were found in Catla catla (Sharma et al., 2017), mussel (Belavgeni and Dailianis, 2017) and Litopenaeus vannamei (Duan et al., 2017). Cytokines, especially TNF-α, are produced during liver injury, subsequently activate iNOS and induce NO release (Carnovale et al., 2000). In general, the activation of transcription factors NF-κB is an indispensable step for iNOS activity increasing, afterward inducing the release of NO (Pautz et al., 2010). In the present study, the expression levels of two major homologs of the components of NF-κB signal pathways, IMD-Relish and Toll-Doral, were both affected by the dietary lipid sources. The mRNA expression of the key gene in NF-κB signal pathway deceased significantly when fish oil was replaced by vegetable oils. It is speculated that both IMD-Relish and Toll-Dorsal signal pathway were activated by oxidative stress, which further caused lipid peroxidation in DFO. This conclusion supported by previous research that shows that oxidative stress is a major factor leading to the phosphorylation of IκB, that subsequently releases NF-κB, which than is translocated to the nucleus to regulate the expression of target genes (Bowie and O’Neill, 2000).

Conclusion

This present study investigated the growth performance and oxidative status of M. rosenbergii fed different lipid sources, and revealed the molecular regulatory mechanism in response to n-3 LC-PUFA. In the present study, vegetable oils replacing dietary fish oil benefited the growth performance, it could be attributed to the modified hepatopancreas oxidative status of prawns fed with vegetable oils, of which the fatty acid profile is different from fish oil. A putative mechanism to explain our results is shown in Figure 7. Excess n-3 PUFA causes lipid peroxidation, which exposes to oxidative stress. This leads to increased levels of hemolymph proinflammatory cytokines (TNF-α, INF-γ, IL-1, and IL-6), then hepatopancreatic cellular damage, and apoptosis occur. NF-κB signal pathway is activated to induce iNOS expression, which would induce NO production. NO would remit the oxidative stress. Thus, vegetable oils, such as soybean oil and RO might be the better lipid sources for M. rosenbergii than fish oil, which might be attributed to modified oxidative status induced by NF-κB-NO signal pathway.

FIGURE 7

Figure 7. NF-κB/NO signaling mechanism in response to different lipid sources.

Data Availability Statement

The authors declared that the raw data underlying the conclusions made in the manuscript will be made available to other researchers by request.

Ethics Statement

This study has been reviewed and approved by the Committee of the Animal Research Institute of Nanjing Agricultural University, China [permit number: SYXK (Su) 2011-0036]. The care and use of animals followed Animal Research Institute Committee guidelines of Nanjing Agricultural University, China.

Author Contributions

CS contributed in the areas of experimental design, sampling, data analysis, and write-up. BL and QZ contributed to the experimental design and manuscript review. ZX and FS contributed to feeding and cultivating of experimental shrimps, sampling and statistics. HZ contributed to feed production, feeding of shrimps and sampling.

Funding

This work is supported by the Central Public-Interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS (CS, grant number: 2018JBFR02), the Natural Science Foundation of Jiangsu Province for Youths (CS, grant number: BK20180172), National Key R&D Program of China (BL, grant number: 2019YFD0900200), and China Agriculture Research System (BL, grant number: CARS-48). All the funders had no role in the design, analysis or writing of this manuscript.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Abbreviations

AA, arachidonic acid; ASA, anti-superoxide anion; DFO, group fed the diets included 6% fish oil; DRO, group fed the diets included 6% rapeseed oil; DSO, group fed the diets included 6% soybean oil; EFA, essential fatty acids; EPA, eicosapentaenoic acid; FCR, feed conversion ratio; FO, fish oil; IL-1, interleukin-1; IL-6, interleukin-6; IMD, immune deficiency; INF- γ, interferon- γ; iNOS, inducible-type NO-synthase; LA, linoleic acid; LC-PUFA, long-chain polyunsaturated fatty acids; LNA, linolenic acid; OA, oleic acid; RFI, relative feed intake; RO, rapeseed oil; SGR, specific growth rate; SO, soybean oil; TDT, terminal deoxynucleotidyl transferase; TNF- α, tumor necrosis factor- α; TUNEL, transferase-mediated dUTP-biotin nick end labeling; WGR, weight gain rate.

References

Aktan, F. (2004). iNOS-mediated nitric oxide production and its regulation. Life Sci. 75, 639–653.

PubMed Abstract | Google Scholar

Arockiaraj, J., Avin, F. A., Vanaraja, P., Easwvaran, S., Singh, A., Othman, R. Y., et al. (2012). Immune role of MrNFκBI-α, an IκB family member characterized in prawn M. rosenbergii. Fish Shellf. Immunol. 33, 619–625. doi: 10.1016/j.fsi.2012.06.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Atalay, M., Laaksonen, D. E., Khanna, S., Kaliste-Korhonen, E., Hänninen, O., and Sen, C. K. (2000). Vitamin E regulates changes in tissue antioxidants induced by fish oil and acute exercise. Med. Sci. Sports Exerc. 32, 601–607.

PubMed Abstract | Google Scholar

Banerjee, K., and Khemundu, G. R. (2019). Total replacement of fishmeal with indigenously available ingredients from plant sources for culturing Macrobrachium rosenbergii. Aquaculture 512:734277.

Google Scholar

Belavgeni, A., and Dailianis, S. (2017). The role of phosphatidylinositol-3-OH-kinase (PI3-kinase) and respiratory burst enzymes in the [omim][BF4]-mediated toxic mode of action in mussel hemocytes. Fish Shellf. Immunol. 68, 144–153. doi: 10.1016/j.fsi.2017.07.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Bell, J. G., Henderson, R. J., Tocher, D. R., and Sargent, J. R. (2004). Replacement of dietary fish oil with increasing levels of linseed oil: modification of flesh fatty acid compositions in Atlantic salmon (Salmo salar) using a fish oil finishing diet. Lipids 39, 223–232.

PubMed Abstract | Google Scholar

Bell, M. V., and Tocher, D. R. (2009). “Biosynthesis of polyunsaturated fatty acids in aquatic ecosystems: general pathways and new directions,” in Lipids in Aquatic Ecosystems, eds M. Kainz, M. Brett, and M. Arts (New York, NY: Springer), 211–236.

Google Scholar

Betancor, M., Atalah, E., Caballero, M., Benítez-Santana, T., Roo, J., Montero, D., et al. (2011). α-Tocopherol in weaning diets for European sea bass (Dicentrarchus labrax) improves survival and reduces tissue damage caused by excess dietary DHA contents. Aquac. Nutr. 17, e112–e122.

Google Scholar

Bligh, E. G., and Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917.

Google Scholar

Bowie, A., and O’Neill, L. A. (2000). Oxidative stress and nuclear factor-κB activation^∗: a reassessment of the evidence in the light of recent discoveries. Biochem. Pharmacol. 59, 13–23.

Google Scholar

Busse, M. S., Arnold, C. P., Towb, P., Katrivesis, J., and Wasserman, S. A. (2007). A κB sequence code for pathway-specific innate immune responses. EMBO J. 26, 3826–3835.

Google Scholar

Carnovale, C. E., Scapini, C., Alvarez, M. L., Favre, C., Monti, J., and Carrillo, M. C. (2000). Nitric oxide release and enhancement of lipid peroxidation in regenerating rat liver. J. Hepatol. 32, 798–804.

PubMed Abstract | Google Scholar

Chen, K., Li, E., Li, T., Xu, C., Xu, Z., Qin, J. G., et al. (2017). The expression of the Δ6 fatty acyl desaturase-like gene from Pacific white shrimp (Litopenaeus vannamei) under different salinities and dietary lipid compositions. J. Shellf. Res. 36, 501–510.

Google Scholar

Chen, X. M., Guo, G. L., Sun, L., Yang, Q. S., Wang, G. Q., and Zhang, D. M. (2017). Modulatory role of L-carnitine against microcystin-LR-induced immunotoxicity and oxidative stress in common carp. Fish Physiol. Biochem. 43, 1081–1093. doi: 10.1007/s10695-017-0354-3

PubMed Abstract | CrossRef Full Text | Google Scholar

De Gregorio, E., Spellman, P. T., Tzou, P., Rubin, G. M., and Lemaitre, B. (2002). The Toll and Imd pathways are the major regulators of the immune response in Drosophila. EMBO J. 21, 2568–2579.

PubMed Abstract | Google Scholar

Del Rosario González-Baró, M., and Pollero, R. J. (1998). Fatty acid metabolism of Macrobrachium borellii: dietary origin of arachidonic and eicosapentaenoic acids. Compar. Biochem. Physiol. A Mol. Integr. Physiol. 119, 747–752.

Google Scholar

Dixit, V., and Mak, T. W. (2002). NF-κB signaling: many roads lead to Madrid. Cell 111, 615–619.

PubMed Abstract | Google Scholar

Du, Z. Y., Clouet, P., Huang, L. M., Degrace, P., Zheng, W. H., He, J. G., et al. (2010). Utilization of different dietary lipid sources at high level in herbivorous grass carp (Ctenopharyngodon idella): mechanism related to hepatic fatty acid oxidation. Aquac. Nutr. 14, 77–92.

Google Scholar

Duan, Y., Dong, H., Wang, Y., Li, H., Liu, Q., Zhang, Y., et al. (2017). Intestine oxidative stress and immune response to sulfide stress in Pacific white shrimp Litopenaeus vannamei. Fish Shellf. Immunol. 63, 201–207. doi: 10.1016/j.fsi.2017.02.013

PubMed Abstract | CrossRef Full Text | Google Scholar

Forlenza, M., Fink, I. R., Raes, G., and Wiegertjes, G. F. (2011). Heterogeneity of macrophage activation in fish. Dev. Compar. Immunol. 35, 1246–1255.

Google Scholar

Fu, H., Jiang, S., and Xiong, Y. (2012). Current status and prospects of farming the giant river prawn (Macrobrachium rosenbergii) and the oriental river prawn (Macrobrachium nipponense) in China. Aquac. Res. 43, 993–998.

Google Scholar

Hayden, M. S., and Ghosh, S. (2008). Shared principles in NF-kappaB signaling. Cell 132, 344–362. doi: 10.1016/j.cell.2008.01.020

PubMed Abstract | CrossRef Full Text | Google Scholar

Hoffmann, J. A., and Reichhart, J. M. (2002). Drosophila innate immunity: an evolutionary perspective. Nat. Immunol. 3, 121–126.

Google Scholar

Hong, M. Y., Turner, N. D., Murphy, M. E., Carroll, R. J., Chapkin, R. S., and Lupton, J. R. (2015). In vivo regulation of colonic cell proliferation, differentiation, apoptosis, and P27Kip1 by dietary fish oil and butyrate in rats. Cancer Prevent. Res. 8, 1076–1083. doi: 10.1158/1940-6207.CAPR-15-0147

PubMed Abstract | CrossRef Full Text | Google Scholar

Huang, X., Wang, W., and Ren, Q. (2016). Dorsal transcription factor is involved in regulating expression of crustin genes during white spot syndrome virus infection. Dev. Comp. Immunol. 63, 18–26. doi: 10.1016/j.dci.2016.05.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Janssen-Heininger, Y., Jones, J., McMillan, D., Qian, X., Lahue, K., and Van Der Velden, J. (2016). D33 role of oxidative stress in inflammation: Gstp modulates Nf-kappa b activation and pro-inflammatory responses in lung epithelial cells. Am. J. Respir. Crit. Care Med. 193:1.

Google Scholar

Kamarudin, M. S., and Roustaian, P. (2002). Growth and fatty acid composition of freshwater prawn, Macrobrachium rosenbergii, larvae fed diets containing various ratios of cod liver oil–corn oil mixture. J. Appl. Ichthyol. 18, 148–153.

Google Scholar

Kangpanich, C., Pratoomyot, J., and Senanan, W. (2017). Effects of alternative oil sources in feed on growth and fatty acid composition of juvenile giant river prawn (Macrobrachium rosenbergii). Agric. Nat. Resour. 51, 103–108.

Google Scholar

Kim, Y. C., Romano, N., Lee, K. S., Teoh, C. Y., and Ng, W. K. (2013). Effects of replacing dietary fish oil and squid liver oil with vegetable oils on the growth, tissue fatty acid profile and total carotenoids of the giant freshwater prawn, Macrobrachium rosenbergii. Aquac. Res. 44, 1731–1740.

Google Scholar

Lemaitre, B., and Hoffmann, J. (2007). The host defense of Drosophila melanogaster. Annu. Rev. Immunol. 25, 697–743.

PubMed Abstract | Google Scholar

Li, C., Li, H., Chen, Y., Chen, Y., Wang, S., Weng, S. P., et al. (2015). Activation of Vago by interferon regulatory factor (IRF) suggests an interferon system-like antiviral mechanism in shrimp. Sci. Rep. 5:15078. doi: 10.1038/srep15078

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, Q., Ai, Q., Mai, K., Xu, W., and Zheng, Y. (2013). A comparative study: in vitro effects of EPA and DHA on immune functions of head-kidney macrophages isolated from large yellow croaker (Larmichthys crocea). Fish Shellfish Immunol. 35, 933–940. doi: 10.1016/j.fsi.2013.07.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, Y., Liang, X., Zhang, Y., and Gao, J. (2016). Effects of different dietary soybean oil levels on growth, lipid deposition, tissues fatty acid composition and hepatic lipid metabolism related gene expressions in blunt snout bream (Megalobrama amblycephala) juvenile. Aquaculture 451, 16–23.

Google Scholar

Liang, Q., Zheng, J., Zuo, H., Li, C., Niu, S., Yang, L., et al. (2017). Identification and characterization of an interleukin-16-like gene from pacific white shrimp Litopenaeus vannamei. Dev. Comp. Immunol. 74, 49–59. doi: 10.1016/j.dci.2017.04.011

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, B., Ge, X., Xie, J., Xu, P., He, Y., Cui, Y., et al. (2012). Effects of anthraquinone extract from Rheum officinale Bail on the physiological responses and HSP70 gene expression of Megalobrama amblycephala under Aeromonas hydrophila infection. Fish Shellfish Immunol. 32, 1–7. doi: 10.1016/j.fsi.2011.02.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Livak, K. J., and Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25, 402–408.

Google Scholar

Lu, K. L., Xu, W. N., Li, J. Y., Li, X. F., Huang, G. Q., and Liu, W. B. (2013). Alterations of liver histology and blood biochemistry in blunt snout bream Megalobrama amblycephala fed high-fat diets. Fish. Sci. 79, 661–671.

Google Scholar

Marinello, P. C., Panis, C., Silva, T. N. X., Binato, R., Abdelhay, E., Rodrigues, J. A., et al. (2019). Metformin prevention of doxorubicin resistance in MCF-7 and MDA-MB-231 involves oxidative stress generation and modulation of cell adaptation genes. Sci. Rep. 9:5864. doi: 10.1038/s41598-019-42357-w

PubMed Abstract | CrossRef Full Text | Google Scholar

Matés, J. M., Pérez-Gómez, C., and De Castro, I. N. (1999). Antioxidant enzymes and human diseases. Clin. Biochem. 32, 595–603.

PubMed Abstract | Google Scholar

Mekata, T., Sudhakaran, R., Okugawa, S., Inada, M., Kono, T., Sakai, M., et al. (2010). A novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp, Marsupenaeus japonicus. Fish Shellfish Immunol. 28, 571–578. doi: 10.1016/j.fsi.2009.12.020

PubMed Abstract | CrossRef Full Text | Google Scholar

Miao, W., and Ge, X. (2002). Freshwater prawn culture in China: an overview. Aquac. Asia 7, 9–12.

Google Scholar

Moffitt, C. M., and Cajas-Cano, L. (2014). Blue growth: the 2014 FAO state of world fisheries and aquaculture. Fisheries 39, 552–553.

Google Scholar

Muhlhausler, B. S., and Ailhaud, G. P. (2013). Omega-6 polyunsaturated fatty acids and the early origins of obesity. Curr. Opin. Endocrinol. Diabetes Obes. 20, 56–61. doi: 10.1097/MED.0b013e32835c1ba7

PubMed Abstract | CrossRef Full Text | Google Scholar

Mullen, A., Loscher, C. E., and Roche, H. M. (2010). Anti-inflammatory effects of EPA and DHA are dependent upon time and dose-response elements associated with LPS stimulation in THP-1-derived macrophages. J. Nutr. Biochem. 21, 444–450. doi: 10.1016/j.jnutbio.2009.02.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Muralidhar, B., Carpenter, K. L., Müller, K., Skepper, J. N., and Arends, M. J. (2004). Potency of arachidonic acid in polyunsaturated fatty acid-induced death of human monocyte–macrophages: implications for atherosclerosis. Prostaglandins Leukot. Essent. Fatty Acids 71, 251–262.

PubMed Abstract | Google Scholar

Muralisankar, T., Bhavan, P. S., Radhakrishnan, S., Seenivasan, C., Manickam, N., and Shanthi, R. (2014). Effects of dietary supplementation of fish and vegetable oils on the growth performance and muscle compositions of the freshwater prawn Macrobrachium rosenbergii. J. Basic Appl. Zool. 67, 34–39. doi: 10.1016/j.jobaz.2014.09.004

CrossRef Full Text | Google Scholar

Myhrstad, M., Ulven, S. M., Günther, C. C., Ottestad, I., Holden, M., Ryeng, E., et al. (2014). Fish oil supplementation induces expression of genes related to cell cycle, endoplasmic reticulum stress and apoptosis in peripheral blood mononuclear cells: a transcriptomic approach. J. Intern. Med. 276, 498–511. doi: 10.1111/joim.12217

PubMed Abstract | CrossRef Full Text | Google Scholar

Naik, E., and Dixit, V. M. (2011). Mitochondrial reactive oxygen species drive proinflammatory cytokine production. J. Exp. Med. 208, 417–420.

Google Scholar

Nappi, A. J., and Ottaviani, E. (2000). Cytotoxicity and cytotoxic molecules in invertebrates. Bioessays 22, 469–480.

PubMed Abstract | Google Scholar

Nasopoulou, C., Stamatakis, G., Demopoulos, C. A., and Zabetakis, I. (2011). Effects of olive pomace and olive pomace oil on growth performance, fatty acid composition and cardio protective properties of gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax). Food Chem. 129, 1108–1113. doi: 10.1016/j.foodchem.2011.05.086

PubMed Abstract | CrossRef Full Text | Google Scholar

National Research Council [NRC] (2011). Nutrient Requirements of Fish and Shrimp (Pittsburgh: National Academies Press), 107–116.

Google Scholar

Nieto, N. (2007). Ethanol and fish oil induce NFκB transactivation of the collagen α2 (I) promoter through lipid peroxidation–driven activation of the PKC-PI3K-Akt pathway. Hepatology 45, 1433–1445.

Google Scholar

Ottaviani, E., Franchini, A., Kletsas, D., and Franceschi, C. (1996). Presence and role of cytokines and growth factors in invertebrates. Ital. J. Zool. 63, 317–323.

Google Scholar

Pautz, A., Art, J., Hahn, S., Nowag, S., Voss, C., and Kleinert, H. (2010). Regulation of the expression of inducible nitric oxide synthase. Nitric Oxide 23, 75–93. doi: 10.1016/j.niox.2010.04.007

PubMed Abstract | CrossRef Full Text | Google Scholar

Pérez-Matute, P., Marti, A., Martínez, J. A., Fernández-Otero, M. P., Stanhope, K. L., Havel, P. J., et al. (2007). Conjugated linoleic acid inhibits glucose metabolism, leptin and adiponectin secretion in primary cultured rat adipocytes. Mol. Cell. Endocrinol. 268, 50–58.

PubMed Abstract | Google Scholar

Petriella, A. M., Muller, M. I., Fenucci, J. L., and Saez, M. B. (1984). Influence of dietary fatty acids and cholesterol on the growth and survival of the Argentine prawn, Artemesia longinaris Bate. Aquaculture 37, 11–20.

Google Scholar

Piedecausa, M. A., Mazon, M. J., Garcia, G. B., and Hernandez, M. D. (2007). Effects of total replacement of fish oil by vegetable oils in the diets of sharpsnout seabream (Diplodus puntazzo). Aquaculture 263, 211–219.

Google Scholar

Pupe, A., De Haes, P., Rhodes, L., Garmyn, M., Moison, R., van Henegouwen, G. B., et al. (2002). Eicosapentaenoic acid, a n-3 polyunsaturated fatty acid differentially modulates TNF-α, IL-1α, IL-6 and PGE2 expression in UVB-irradiated human keratinocytes. J. Investig. Dermatol. 118, 692–698.

Google Scholar

Qin, N., Tang, T., Liu, X., Xie, S., and Liu, F. (2019). Involvement of a TNF homologue in balancing the host immune system of Macrobrachium nipponense. Int. J. Biol. Macromol. 134, 73–79. doi: 10.1016/j.ijbiomac.2019.05.045

PubMed Abstract | CrossRef Full Text | Google Scholar

Querijero, B. V. L., Teshima, S., Koshio, S., and Ishikawa, M. (1997). Utilization of monounsaturated fatty acid (18: 1n-9, oleic acid) by freshwater prawn Macrobrachium rosenbergii (de Man) juveniles. Aquac. Nutr. 3, 127–139.

Google Scholar

Radhakrishnan, S., Bhavan, P. S., Seenivasan, C., Shanthi, R., and Muralisankar, T. (2014). Replacement of fishmeal with Spirulina platensis, Chlorella vulgaris and Azolla pinnata on non-enzymatic and enzymatic antioxidant activities of Macrobrachium rosenbergii. J. Basic Appl. Zool. 67, 25–33.

Google Scholar

Reigh, R. C., and Stickney, R. R. (1989). Effects of purified dietary fatty acids on the fatty acid composition of freshwater shrimp, Macrobrachium rosenbergii. Aquaculture 77, 157–174.

Google Scholar

Sanders, L. M., Henderson, C. E., Hong, M. Y., Barhoumi, R., Burghardt, R. C., Wang, N., et al. (2004). An increase in reactive oxygen species by dietary fish oil coupled with the attenuation of antioxidant defenses by dietary pectin enhances rat colonocyte apoptosis. J. Nutr. 134, 3233–3238.

PubMed Abstract | Google Scholar

Sharma, J. G., Singh, S. P., and Chakrabarti, R. (2017). Effect of temperature on digestive physiology, immune-modulatory parameters, and expression level of Hsp and LDH genes in Catla catla (Hamilton, 1822). Aquaculture 479, 134–141.

Google Scholar

Shi, Y. (2016). The Primer Sequence of IMD was Obtained from the Thesis: Shi Yanru. Study on Sequences Analysis and Function of IMD and Relish in IMD Signal Pathway in Macrobrachium rosenbergii. Nanjing: Nanjing Normal University.

Google Scholar

Shi, Y. R., Jin, M., Ma, F. T., Huang, Y., Huang, X., Feng, J. L., et al. (2015). Involvement of Relish gene from Macrobrachium rosenbergii in the expression of anti-microbial peptides. Dev. Comp. Immunol. 52, 236–244. doi: 10.1016/j.dci.2015.05.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Srisuk, C., Longyant, S., Senapin, S., Sithigorngul, P., and Chaivisuthangkura, P. (2014). Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii. Fish Shellfish Immunol. 36, 552–562. doi: 10.1016/j.fsi.2013.12.025

PubMed Abstract | CrossRef Full Text | Google Scholar

Tariq, Z., Green, C. J., and Hodson, L. (2014). Are oxidative stress mechanisms the common denominator in the progression from hepatic steatosis towards non-alcoholic steatohepatitis (NASH)? Liver Int. 34, e180–e190. doi: 10.1111/liv.12523

PubMed Abstract | CrossRef Full Text | Google Scholar

Tidwell, J. H., Webster, C. D., Yancey, D. H., and D’Abramo, L. R. (1993). Partial and total replacement of fish meal with soybean meal and distillers’ by-products in diets for pond culture of the freshwater prawn (Macrobrachium rosenbergii). Aquaculture 118, 119–130.

Google Scholar

Tocher, D. R., Dabrowski, K., and Hardy, R. (2010). Fatty acid requirements in ontogeny of marine and freshwater fish. Aquac. Res. 41, 717–732.

Google Scholar

Turchini, G. M., Torstensen, B. E., and WingKeong, N. (2010). Fish oil replacement in finfish nutrition. Rev. Aquac. 1, 10–57.

Google Scholar

Valanne, S., Wang, J. H., and Rämet, M. (2011). The Drosophila toll signaling pathway. J. Immunol. 186, 649–656.

Google Scholar

Violi, F., Marino, R., Milite, M., and Loffredo, L. (1999). Nitric oxide and its role in lipid peroxidation. Diabetes Metab. Res. Rev. 15, 283–288.

PubMed Abstract | Google Scholar

Wang, P. H., Wan, D. H., Pang, L. R., Gu, Z. H., Qiu, W., Weng, S. P., et al. (2012). Molecular cloning, characterization and expression analysis of the tumor necrosis factor (TNF) superfamily gene, TNF receptor superfamily gene and lipopolysaccharide-induced TNF-α factor (LITAF) gene from Litopenaeus vannamei. Dev. Comp. Immunol. 36, 39–50. doi: 10.1016/j.dci.2011.06.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Weldon, S. M., Mullen, A. C., Loscher, C. E., Hurley, L. A., and Roche, H. M. (2007). Docosahexaenoic acid induces an anti-inflammatory profile in lipopolysaccharide-stimulated human THP-1 macrophages more effectively than eicosapentaenoic acid. J. Nutr. Biochem. 18, 250–258.

PubMed Abstract | Google Scholar

Wieckowska, A., Zein, N. N., Yerian, L. M., Lopez, A. R., Mccullough, A. J., and Feldstein, A. E. (2006). In vivo assessment of liver cell apoptosis as a novel biomarker of disease severity in nonalcoholic fatty liver disease. Hepatology 44, 27–33.

PubMed Abstract | Google Scholar

Xu, Y., Huang, Y., and Cai, S. (2017). Characterization and function analysis of interleukin-1 receptor-associated kinase-1 (IRAK-1) from Fenneropenaeus penicillatus. Fish Shellfish Immunol. 61, 111–119. doi: 10.1016/j.fsi.2016.12.030

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, Z., Guo, Z., Ji, L., Zeng, Q., Wang, Y., Yang, X., et al. (2013). Cloning and tissue distribution of a fatty acyl Δ6-desaturase-like gene and effects of dietary lipid levels on its expression in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis). Comp. Biochem. Physiol. B Biochem. Mol. Biol. 165, 99–105. doi: 10.1016/j.cbpb.2013.03.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhu, H., He, A., Chen, L., Qin, J., Li, E., Li, Q., et al. (2017). Effects of dietary lipid level and n-3/n-6 fatty acid ratio on growth, fatty acid composition and lipid peroxidation in Russian sturgeon Acipenser gueldenstaedtii. Aquac. Nutr. 23, 879–890.

Google Scholar