- 1Department of Biological Sciences, University of Manitoba, Winnipeg, MB, Canada
- 2Technology Specialist, Fish and Richardson P.C., Minneapolis, MN, United States
A regulatory protein (sarcolipin or SLN) bound to SERCA pumps in the sarcoplasmic reticulum (SR) of cardiac and skeletal muscle has been shown to increase heat production in vitro. Here, we review recent work in this area, assess the potential of in vivo heat generation by this mechanism, and advocate that a comparative approach is the best path toward resolving many outstanding questions on the physiological function, regulation, and evolution of SLN.
Introduction
Precise control of cytosolic Ca2+ levels underlies the modulation of optimal contraction strengths, frequencies, and relaxation rates by cardiac and skeletal muscle over a wide range of activities. This regulation is largely achieved by metering the release of SR Ca2+ stores into the cytosol via ryanodine receptor channels and the subsequent uptake of Ca2+ by sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pumps. Briefly, SERCA harnesses the phosphate bond energy of one ATP molecule to translocate two Ca2+ ions into the SR lumen, thereby generating a ~15,000-fold (1.5 mM vs. 0.1 μM) lumen-to-cytosol Ca2+ concentration gradient in resting muscle (Toyoshima and Inesi, 2004). Several single-pass transmembrane peptides—including phospholamban, myoregulin, and sarcolipin (SLN)—interact with SERCA isoforms to modify Ca2+ uptake in a tissue specific manner. SLN, for example, is a relatively short (31 amino acid) helical peptide that was initially shown to alter Ca2+ uptake kinetics by inhibiting SERCA1a and SERCA2a activity in the atria and skeletal muscles of mammals (Odermatt et al., 1998; MacLennan et al., 2003). Subsequent in vitro evidence that SLN increases the heat generated by SERCA by partially uncoupling Ca2+ re-sequestration from ATP hydrolysis in rabbit and mouse skeletal muscle (Smith et al., 2002; Mall et al., 2006) has led to the hypothesis that SLN contributes to non-shivering thermogenesis (NST) in vivo (Bal et al., 2012). It should be noted that this proposed heat generating process is mechanistically distinct from, and hence unrelated to, known Ca2+-linked thermogenic processes in skeletal muscle, which will not be further discussed here, such as futile Ca2+ cycling in the non-contractile extraocular muscles of regionally endothermic billfishes and ryanodine receptor mutations associated with malignant hyperthermia. While there remains a lack of consensus regarding adaptive muscle NST in mammals by any mechanism, the proposal that SLN increases energy turnover in the skeletal muscles where it is expressed has generated substantial interest in the biomedical research community as a potential target for obesity and other metabolic syndromes. To date, however, studies on the structure, function, and regulation of SLN, its interaction with SERCA, and its potential role in facultative muscle NST predominantly remain limited to a few model systems that reside within the same mammalian clade (Glires; rodents and rabbits). While this work is tantalizing, several key mechanistic aspects remain unanswered such as (1) whether uncoupling of Ca2+ transport from ATP hydrolysis in SERCA primarily arises from slippage of the SERCA-bound Ca2+ ions into the cytosol after ATP hydrolysis or from passive leak of luminal Ca2+ back into the cytosol through SERCA (Figure 1A), (2) what region(s) and/or residue(s) of SLN and SERCA are involved in this uncoupling, and (3) whether SLN-mediated Ca2+ uncoupling occurs in resting muscle when SR luminal Ca2+ concentration is high and cytosolic [Ca2+] is low, or in actively-contracting and/or shivering muscle when cytosolic Ca2+ is elevated (de Meis, 2001; Smith et al., 2002; Inesi and Tadini-Buoninsegni, 2014). Also unclear are broader questions pertaining to how SLN-mediated Ca2+ uncoupling evolved, and its occurrence and potential thermogenic importance—i.e., how much heat is produced relative to shivering—across the mammalian phylogeny (and beyond).
![www.frontiersin.org](https://www.frontiersin.org/files/Articles/368603/fphys-09-00714-HTML/image_m/fphys-09-00714-g001.jpg)
Figure 1. Proposed mechanism of sarcolipin in skeletal muscle non-shivering thermogenesis, and its genetic evolution. (A) The two functional mechanisms by which SLN is postulated to return SERCA-bound Ca2+ to the cytosol following ATP hydrolysis: enzyme slippage and passive leak (right complex). (B) Primary mechanisms of ATP hydrolysis in a typical skeletal muscle fiber following a nervous stimulation: (1) Na+-K+-ATPase, (2) myosin-ATPase, and (3) SERCA Ca2+-ATPase. Note that while some heat is liberated by these ATPases, most muscle thermogenesis is via mitochondrial catabolic processes which generate the proton motive force required for ATP synthesis. (C) Mammalian phylogenetic tree denoting potentially relevant SLN residue substitutions (black), start codon mutations (blue), and deletions (red) based on data in Gaudry et al. (2017). SLN residue replacement and deletion events are not dated and are placed on the phylogenetic branches in sequential order. The height of collapsed branches (gray triangles) is proportional to the number of SLN sequences available for each clade. Note that published functional studies pertaining to SLN-NST have been restricted to a few model species in the Glires clade (predominantly mice). Silhouettes are taken from phylopic.org (image credits: megabat, Oren Peles/vectorized by Yan Wong; rabbit and sloth, Sarah Werning; https://creativecommons.org/licenses/by/3.0/).
Heat Yes, But How Much?
It is imperative to delineate the potential amount of heat generated via SLN-mediated NST from that of other ATP consuming processes coupled to Ca2+ turnover during a muscle contraction-relaxation cycle. These include activation of sarcolemma Na+-K+-ATPases for membrane repolarization, myosin-ATPases for cross-bridge relaxation, and SERCA Ca2+-ATPases for Ca2+ sequestration (Figure 1B). As SLN is not associated with the former two ATPases, back of the napkin calculations suggest its thermogenic contribution is limited to a small fraction of that liberated by shivering. For simplicity, if we arbitrarily assume that 1,000 Ca2+ ions are released into the cytosol following nervous activation, then transporting these Ca2+ ions back into the SR will hydrolyze 500 ATP (assuming no Ca2+ backflux or leak), with an additional 750–1,167 ATP hydrolyzed by the Na+-K+ and myosin ATPases (these two ATPases are estimated to contribute ~60–70% of the energetic cost of an isometric muscle contraction; Barclay et al., 2007). For SLN to not interfere with muscle relaxation speeds, and hence maximal contraction frequencies—an especially important consideration for small mammals—SLN uncoupling is likely to predominantly operate when SR luminal concentrations are elevated (Inesi and Tadini-Buoninsegni, 2014). Thus, assuming Ca2+ slippage/leak occurs at a cytosolic [Ca2+] low enough to preclude cross-bridge formation—say below 100 Ca2+ ions in our example—and that the Ca2+/ATP coupling ratio is lowered by 35% (Bombardier et al., 2013), then this mechanism would only hydrolyze an additional ~25 ATP, or ~2% of the heat produced by a single contraction-relaxation cycle. Results of in vitro biochemical assays demonstrate that SERCA activity is almost undetectable at Ca2+ levels found in resting muscle (Gorski et al., 2013; Sahoo et al., 2013). Nonetheless, even if we assume that SLN lowers net Ca2+ pumping efficiency to zero in this state, then >3-fold more Ca2+ ions than are released during a single muscle contraction would need to “slip” for SLN to generate an equivalent amount of heat within the same timeframe. Of course, this latter scenario also requires that cytosolic Ca2+ levels remain below the contraction threshold, whereby few SERCA Ca2+ binding sites would be populated relative to that following muscle contraction (Barclay et al., 2007). Taken together, these calculations question whether SLN-NST can contribute meaningfully to thermogenesis relative to shivering and other non-shivering processes (e.g., brown adipose tissue).
Evidence for SLN-Mediated Non-Shivering Thermogenesis is Inconclusive
Crucially, heat released via the SLN and SERCA complex has been shown to increase linearly with SLN concentration in vitro (Mall et al., 2006), which current data suggests varies markedly among skeletal muscles in rodents. Briefly, moderate concentrations of SLN are present in small muscles of mice such as the soleus and diaphragm that are predominantly composed of slow-twitch oxidative fibers, though (nearly) undetectable in fast-twitch glycolytic fibers that form the bulk of the limb musculature (e.g., quadriceps; Vangheluwe et al., 2005; Babu et al., 2007; Bombardier et al., 2013). Consequently, any NST linked to SLN may be expected to be imperceptible in these small mammals. This conclusion is in line with recent work on SLN knock-out mice that revealed no differences in body mass, food intake, or resting whole body and isolated soleus muscle O2 consumption rates relative to wild-type littermates, despite indications that SLN reduced the apparent Ca2+/ATP coupling ratio within the soleus (but not fast-twitch extensor digitorum longus muscles) by up to 35% (Bombardier et al., 2013). Similarly, SLN overexpressing mice exhibited no differences in body mass or energy expenditure, even on high fat diets, relative to wild-type individuals whose muscles contained ~25-fold lower SLN concentrations (Butler et al., 2015). It should be noted, however, that these findings are not universal, as pair-fed SLN knock-out mice gained weight relative to wild-type mice, while SLN overexpression resulted in weight loss and an increased rate of O2 consumption (Maurya et al., 2015). The basis for these contradictory findings remains unclear, though differences in O2 consumption may have arisen in part due to values being presented in a mass-specific basis for the latter study. Nonetheless, results of the Maurya study are ostensibly bolstered by several mouse studies that contend that loss of SLN hinders muscle-based NST (Bal et al., 2012, 2017; Rowland et al., 2015), although it is also conceivable that the observed effects of SLN ablation arise from an impaired shivering response, altered Ca2+ signaling pathways, and/or compromised Ca2+ handling.
Unfortunately, in vivo experiments in support of muscle NST that involved curare (e.g., Bal et al., 2012) are inconclusive as none of the animals required artificial respiration, thus indicating that shivering may not have been completely blocked. Additional support for a thermogenic role for SLN is that cold acclimation mitigates the natural developmental reduction in SLN levels in neonatal mice and results in elevated SLN transcription in slow-twitch adult skeletal muscle (Pant et al., 2015). However, this interpretation is complicated by studies conducted on non-cold stressed wheel running mice whose soleus SLN mRNA expression profiles show a distinct exercise effect, being 2- to 3-fold higher than found in sedentary mouse muscle (de Snoo, 2009). Consequently, the observed elevations in muscle SLN levels in cold challenged mice may simply reflect a shivering induced “training effect.” While additional research in this area is required, the findings of the wheel running mice studies are not consistent with an adaptive thermogenic role for SLN, as elevated ATP hydrolysis due to futile Ca2+ pumping would be expected to impair exercise performance (see below).
SLN Expression and Evolutionary Analyses
Previous work has shown SLN mRNA and/or protein levels to be several magnitudes higher in both slow and fast twitch skeletal muscles of rabbits, pigs, dogs, and humans than lab rodents, leading to suggestions that SLN-NST may be of greater thermogenic importance in larger mammals (Barbot et al., 2016). However, given that larger mammals also possess lower surface-area-to-volume ratios, thicker insulation, and the ability to further minimize heat loss via countercurrent heat exchangers, this contention is puzzling. Similarly, implications that larger mammals may exhibit a stronger reliance on SLN-NST due to small (or even absent) depots of thermogenic brown adipose tissue (BAT) are unsatisfactory, as it is precisely for the reasons outlined above that these species have a reduced reliance on BAT thermogenesis (i.e., a reduction in BAT mass does not need to be counterbalanced by other heat generating mechanisms). Indeed, recent work has illustrated that ancient magnitude increases in body mass in five mammalian lineages [cetaceans (whales and dolphins), elephantids, sirenians (sea cows), hyracoids (hyraxes), and equids (horses and kin)] were temporally coupled with inactivation of thermogenic UCP1 (Gaudry et al., 2017), suggesting that costs of maintaining an elevated thermogenic capacity outweighed its benefits.
If SLN plays a prominent thermoregulatory role in mammals, then this locus may be expected to have evolved under relaxed selection or potentially even be pseudogenized in large or tropically distributed species and/or mammalian lineages lacking a functional UCP1 protein. The latter assemblage offers particularly good model systems as members of some UCP1 lacking lineages (cetaceans, woolly mammoths, Steller's sea cows, and horses) have nonetheless evolved extreme cold tolerance while others (pangolins, xenarthrans, hyraxes, pigs, and extant sea cows) are notoriously cold intolerant (even often as adults). In contrast to such expectations, not only is the SLN locus functionally intact in each of these cases (i.e., able to encode a ~31 unit peptide), but its primary structure has remained exceptionally well conserved despite highly divergent body sizes and thermoregulatory capacities among species. For example, UCP1-lacking elephants, whales, pigs, and hyraxes all possess identical SLN peptides to those of rabbits, while SLN sequences of “proto-endothermic” tenrecs and golden moles precisely match those of cold tolerant deer mice and meadow voles (cf. Figure S4 of Gaudry et al., 2017). This remarkable degree of sequence conservation—over evolutionary timescales exceeding 65 million years, and in large bodied and tropical species for which NST should have no apparent adaptive benefit—is not readily compatible with a thermogenic function. Intriguingly, the start codon of SLN is mutated in weakly endothermic sloths (Figure 1C), which moreover have nonfunctional UCP1 (Gaudry et al., 2017), though its primary structure remains highly conserved; whether the start codon mutation inhibits protein translation or is rescued by alternative splicing remains unknown and should be examined.
While there is evidence that SLN interaction with SERCA results in an elevated rate of ATP hydrolysis in vitro, taken together, the above findings argue that the primary role of SLN in skeletal muscle of mammals is not thermoregulatory in nature. Nonetheless, SLN expression and in vitro studies on a range of large-bodied mammals, including species lacking functional UCP1, are required to confirm or refute the above contentions. An examination of representative marsupials and monotremes are also required to assess the thermogenic potential of SLN across Mammalia and better pinpoint if and when the proposed thermogenic function arose in evolutionary time. Finally, it is worth noting that the high expression level of SLN in mammalian atria—the concentration of which is 10-to 1,000-fold higher than in the soleus of rodents (Babu et al., 2007; Bombardier et al., 2013)—is also difficult to envision within a thermogenic framework, though in line with a role in modulating intracellular Ca2+ homeostasis by modulating the (apparent) Ca2+ affinity of SERCA. Indeed, both elevated and lowered atrial SLN concentrations are linked to cardiac pathologies (Babu et al., 2007).
How is SLN Regulated?
Surprisingly, the mechanism underling SERCA inhibition by SLN is also equivocal. For example, some labs report a decrease in SERCA's apparent Ca2+ affinity in the presence of SLN (Hellstern et al., 2001; Buck et al., 2003; Buffy et al., 2006; Gorski et al., 2013) whereas others do not observe a change in Ca2+ affinity and only observe a decrease in Ca2+ uptake (Smith et al., 2002; Sahoo et al., 2013). There are several potential reasons for these discordant observations including the use of different assays to measure ATP hydrolysis as well as the use of different test species employed (with different SLN and SERCA sequences). It is essential to both understand and reconcile these inconsistencies to determine the mechanism of inhibition, and whether or not this process is linked to thermogenic Ca2+ uncoupling/leak. Similarly, comparative (and, potentially, site-directed mutagenesis) studies are also required to elucidate what component of the SLN peptide is involved in thermogenesis. Two recent studies have implicated the cytosol-oriented N-terminus to underlie Ca2+ uncoupling (Sahoo et al., 2015; Autry et al., 2016). Specifically, rabbit SLN residues Glu2 and Glu7 are predicted to form salt-bridges with Arg324 and Lys328 of rabbit SERCA1a, thereby perturbing one of the SERCA Ca2+ binding sites (E309 on helix M4), although only Glu7 was predicted as necessary to induce the uncoupling structural change (Autry et al., 2016). Dicke (2017) has questioned the role of this region in uncoupling due to a substantial degree of sequence variability among mammals (e.g., MGINTRE7 in catarrhine primates vs. MERSTQE7 in most rodents; Gaudry et al., 2017), and it remains unknown whether in vitro SLN Ca2+/ATP uncoupling is compromised by the Glu2 → Gly2 replacement in human, carnivore, and aardvark SLN, the Glu2 → Asp2 replacement in vesper bats, or the Glu7 → Asp7 replacement in sloths, dugonid sea cows, and false vampire bats (Figure 1C). It should be further noted that Xenopus and zebrafish—for which a non-shivering thermogenic mechanism would be most unexpected—possess the identical SLN N-terminal MERSTQE motif found in rodents (their SERCA proteins also contain Arg324 and Lys328). By contrast, the luminal C-terminal sequence (27RSYQY31)—which underlies both the proper SR/ER cellular localization of SLN and its apparent inhibition of SERCA (Gramolini et al., 2004; Gorski et al., 2013)—is nearly 100% conserved among mammals, and thus may be a better candidate for an uncoupling function (Dicke, 2017). Importantly, two C-terminal residues (Arg27 and Tyr31) that are proposed to be essential for SLN inhibition (Gorski et al., 2013) are only found in mammals, with other vertebrates having Lys27 and Glu/Asp31 at these sites, except falcons which have Gln31 (Montigny et al., 2014; Gaudry et al., 2017). Interestingly, Tyr31 → Ser31 substitutions appear to have evolved independently at least three times in mammals (twice in bats and again in flying lemurs), while this C-terminal residue is deleted in equids (Figure 1C). These species accordingly provide natural model systems to help evaluate the potential role of the cytosolic (N-terminus) and luminal (C-terminus) regions of SLN in both decreasing SERCA's apparent Ca2+ affinity and uncoupling of Ca2+ transport.
In addition to supplying sufficient heat to help maintain thermal balance in sub-thermoneutral temperatures, a key feature for any adaptive, facultative thermogenic process is the ability to rapidly activate it once the need arises and inactivate it once additional thermogenesis is no longer required. If, for example, SLN-NST is simply an unregulated by-product associated with Ca2+ pumping during muscle contraction, then this mechanism would become maladaptive in many circumstances such as during exercise in temperatures near or above the lower limit of the thermoneutral zone, as this supplemental heat production would tax heat-dissipating processes thereby promoting hyperthermia-induced muscle fatigue and impaired motor performance (Silva, 2011; Nybo et al., 2014). To our knowledge, no studies have yet demonstrated that SLN-NST can be activated/inactivated independently from muscle contraction, let alone have identified the molecular mechanism by which this process can be mediated. While phosphorylation of one or more residues (Ser4 and/or Thr5) within the cytosolic domain have been shown to relieve the inhibitory effect of SLN on cardiac SERCA pumps (Gramolini et al., 2004; Bhupathy et al., 2009), likely via structural changes to the SLN-SERCA complex, it remains unknown whether this mechanism alters uncoupling activity (Autry et al., 2016; Barbot et al., 2016). However, primary sequence analysis of available data reveal that either one or both of Ser4 and Thr5 are—with one exception (equids)—universally present from fish to mammals (Barbot et al., 2016; Gaudry et al., 2017), indicating that this inhibitory mechanism of control is likely both ancient and highly conserved. Key insights into this regulation may thus be provided by studies on horses/donkeys whose SLN peptides (in addition to lacking the C-terminal Tyr31 residue; see above) also lack the potential for reversible phosphorylation (one of the two residues at positions 4–5 is deleted, while the other exhibits a dephospho-mimetic Thr→Arg substitution; Figure 1C).
In addition to phosphorylation as a post-translational modification, it was recently shown that Cys9 of native SLN from rabbits and pigs was S-palmitoylated and S-oleylated (Montigny et al., 2014), and that depalmitoylation treatment increased Ca2+-ATPase activity of rabbit SR by 30%. Rodents, by contrast, possess Phe9 and show a smaller increase in Ca2+-ATPase activity following depalmitoylation (20%; Montigny et al., 2014), suggesting a potential (albeit limited) role for this residue position in the regulation of SLN activity. However, the presence of Cys9—found in mammals ranging from rabbits, bats, kangaroos, and pangolins to elephants and whales (Figure 1C)—does not appear to be linked with either body mass or metabolic intensity. While this phylogenetic distribution is consistent with a pattern of both repeated (convergent) evolution of Cys9 and its reversion back to the ancestral Phe9 found in the majority of vertebrate species, the potential physiological and thermogenic effects of this residue replacement are yet to be assessed.
Conclusions
While there can be little doubt that the interaction of SLN with SERCA lowers the metabolic efficiency of Ca2+ transport in vitro, unequivocal support for an adaptive thermogenic role in vivo is lacking. Suggestions that SLN plays any meaningful thermogenic role in larger bodied mammals (including humans) or non-mammalian vertebrates (e.g., birds) is also without empirical evidence and remains purely speculative. Comparative studies taking advantage of naturally occurring variability in SLN concentrations and primary sequence among vertebrates provide ideal avenues to explore the function of this regulatory peptide, and its evolution.
Author Contributions
KC drafted the manuscript, prepared the figures, and approved the final version. AD contributed to manuscript writing, provided important interpretations, critically revised the work, and provided final approval of the opinion content.
Conflict of Interest Statement
AD was employed by Fish & Richardson P.C. at the time of writing. The ideas and opinions in the article are her own, and predominantly arose from biophysical research on SLN interactions with SERCA at the University of Minnesota during the course of her Ph.D. These viewpoints have not been vetted with the law firm where she works or its clients, and do not represent the positions of the firm, its lawyers, or any of its clients. None of this writing is intended as legal advice and it should not be taken as such.
The remaining author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
KC is funded by the Natural Sciences and Engineering Research Council (NSERC) of Canada (RGPIN/6562-2016). We thank Mike Autry, Jay Treberg, Vitaly Vostrikov, Todd Duhamel, and Jens Franck for constructive comments on an earlier version of this paper.
References
Autry, J. M., Thomas, D. D., and Espinoza-Fonseca, L. M. (2016). Sarcolipin promotes uncoupling of the SERCA Ca2+ pump by inducing a structural rearrangement in the energy-transduction domain. Biochemistry 55, 6083–6086. doi: 10.1021/acs.biochem.6b00728
Babu, G. J., Bhupathy, P., Carnes, C. A., Billman, G. E., and Periasamy, M. (2007). Differential expression of sarcolipin protein during muscle development and cardiac pathophysiology. J. Mol. Cell. Cardiol. 42, 215–222. doi: 10.1016/j.yjmcc.2007.05.009
Bal, N. C., Maurya, S. K., Sopariwala, D. H., Sahoo, S. K., Gupta, S. C., Shaikh, S. A., et al. (2012). Sarcolipin is a newly identified regulator of muscle-based thermogenesis in mammals. Nat. Med. 18, 1575–1579. doi: 10.1038/nm.2897
Bal, N. C., Singh, S., Reis, F. C. G., Maurya, S. K., Pani, S., Rowland, L. A., et al. (2017). Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice. J. Biol. Chem. 292, 16616–16625. doi: 10.1074/jbc.M117.790451
Barbot, T., Montigny, C., Decottignies, P., le Maire, M., Jaxel, C., Jamin, N., et al. (2016). “Functional and structural insights into sarcolipin, a regulator of the sarco-endoplasmic reticulum Ca2+-ATPases,” in Regulation of Ca2+-ATPases, V-ATPases and F-ATPases, eds S. Chakraborti and N.S. Dhalla (Cham: Springer International Publishing), 153–186.
Barclay, C. J., Woledge, R. C., and Curtin, N. A. (2007). Energy turnover for Ca2+ cycling in skeletal muscle. J. Muscle Res. Cell Motil. 28, 259–274. doi: 10.1007/s10974-007-9116-7
Bhupathy, P., Babu, G. J., Ito, M., and Periasamy, M. (2009). Threonine-5 at the N-terminus can modulate sarcolipin function in cardiac myocytes. J. Mol. Cell. Cardiol. 47, 723–729. doi: 10.1016/j.yjmcc.2009.07.014
Bombardier, E., Smith, I. C., Vigna, C., Fajardo, V. A., and Tupling, A. R. (2013). Ablation of sarcolipin decreases the energy requirements for Ca2+ transport by sarco (endo) plasmic reticulum Ca2+-ATPases in resting skeletal muscle. FEBS Lett. 587, 1687–1692. doi: 10.1016/j.febslet.2013.04.019
Buck, B., Zamoon, J., Kirby, T. L., DeSilva, T. M., Karim, C., Thomas, D., et al. (2003). Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies. Protein Expr. Purif. 30, 253–261. doi: 10.1016/S1046-5928(03)00127-X
Buffy, J. J., Buck-Koehntop, B. A., Porcelli, F., Traaseth, N. J., Thomas, D. D., and Veglia, G. (2006). Defining the intramembrane binding mechanism of sarcolipin to calcium ATPase using solution NMR spectroscopy. J. Mol. Biol. 358, 420–429. doi: 10.1016/j.jmb.2006.02.005
Butler, J., Smyth, N., Broadbridge, R., Council, C. E., Lee, A. G., Stocker, C. J., et al. (2015). The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity. Arch. Biochem. Biophys. 569, 26–31. doi: 10.1016/j.abb.2015.01.027
de Meis, L. (2001). Uncoupled ATPase activity and heat production by the sarcoplasmic reticulum Ca2+-ATPase. Regulation by ADP. J. Biol. Chem. 276, 25078–25087. doi: 10.1074/jbc.M103318200
de Snoo, M. (2009). Responses of Mouse Skeletal Muscle to Endurance Exercise: Functional, Metabolic, and Genomic Adaptations. Ph.D. thesis, Utrecht University, Utrecht.
Dicke, A. (2017). Biophysical Characterization of Interactions Between Two Membrane Proteins: SERCA and Sarcolipin. Ph.D. thesis, University of Minnesota, Minneapolis, MN.
Gaudry, M. J., Jastroch, M., Treberg, J. R., Hofreiter, M., Paijmans, J. L. A., Starrett, J., et al. (2017). Inactivation of thermogenic UCP1 as a historical contingency in multiple placental mammal clades. Sci. Adv. 3:E1602878. doi: 10.1126/sciadv.1602878
Gorski, P. A., Glaves, J. P., Vangheluwe, P., and Young, H. S. (2013). Sarco (endo) plasmic reticulum calcium ATPase (SERCA) inhibition by sarcolipin is encoded in its luminal tail. J. Biol. Chem. 288, 8456–8467. doi: 10.1074/jbc.M112.446161
Gramolini, A. O., Kislinger, T., Asahi, M., Li, W., Emili, A., and MacLennan, D. H. (2004). Sarcolipin retention in the endoplasmic reticulum depends on its C-terminal RSYQY sequence and its interaction with sarco (endo) plasmic Ca2+-ATPases. Proc. Natl. Acad. Sci. U.S.A. 101, 16807–16812. doi: 10.1073/pnas.0407815101
Hellstern, S., Pegoraro, S., Karim, C. B., Lustig, A., Thomas, D. D., Moroder, L., et al. (2001). Sarcolipin, the shorter homologue of phospholamban, forms oligomeric structures in detergent micelles and in liposomes. J. Biol. Chem. 276, 30845–30852. doi: 10.1074/jbc.M102495200
Inesi, G., and Tadini-Buoninsegni, F. (2014). Ca2+/H+ exchange, lumenal Ca2+ release and Ca2+/ATP coupling ratios in the sarcoplasmic reticulum ATPase. J. Cell Commun. Signal. 8, 5–11. doi: 10.1007/s12079-013-0213-7
MacLennan, D. H., Asahi, M., and Tupling, A. R. (2003). The regulation of SERCA-type pumps by phospholamban and sarcolipin. Ann. N.Y. Acad. Sci. 986, 472–480. doi: 10.1111/j.1749-6632.2003.tb07231.x
Mall, S., Broadbridge, R., Harrison, S. L., Gore, M. G., Lee, A. G., and East, J. M. (2006). The presence of sarcolipin results in increased heat production by Ca2+-ATPase. J. Biol. Chem. 281, 36597–36602. doi: 10.1074/jbc.M606869200
Maurya, S. K., Bal, N. C., Sopariwala, D. H., Pant, M., Rowland, L. A., Shaikh, S. A., et al. (2015). Sarcolipin is a key determinant of the basal metabolic rate, and its overexpression enhances energy expenditure and resistance against diet-induced obesity. J. Biol. Chem. 290, 10840–10849. doi: 10.1074/jbc.M115.636878
Montigny, C., Decottignies, P., Le Maréchal, P., Capy, P., Bublitz, M., Olesen, C., et al. (2014). S-palmitoylation and s-oleoylation of rabbit and pig sarcolipin. J. Biol. Chem. 289, 33850–33861. doi: 10.1074/jbc.M114.590307
Nybo, L., Rasmussen, P., and Sawka, M. N. (2014). Performance in the heat—physiological factors of importance for hyperthermia-induced fatigue. Compr. Physiol. 4, 657–689. doi: 10.1002/cphy.c130012
Odermatt, A., Becker, S., Khanna, V. K., Kurzydlowski, K., Leisner, E., Pette, D., et al. (1998). Sarcolipin regulates the activity of SERCA1, the fast-twitch skeletal muscle sarcoplasmic reticulum Ca2+-ATPase. J. Biol. Chem. 273, 12360–12369. doi: 10.1074/jbc.273.20.12360
Pant, M., Bal, N. C., and Periasamy, M. (2015). Cold adaptation overrides developmental regulation of sarcolipin expression in mice skeletal muscle: SOS for muscle-based thermogenesis? J. Exp. Biol. 218, 2321–2325. doi: 10.1242/jeb.119164
Rowland, L. A., Bal, N. C., Kozak, L. P., and Periasamy, M. (2015). Uncoupling protein 1 and sarcolipin are required to maintain optimal thermogenesis, and loss of both systems compromises survival of mice under cold stress. J. Biol. Chem. 290, 12282–12289. doi: 10.1074/jbc.M115.637603
Sahoo, S. K., Shaikh, S. A., Sopariwala, D. H., Bal, N. C., Bruhn, D. S., Kopec, W., et al. (2015). The N terminus of sarcolipin plays an important role in uncoupling sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) ATP hydrolysis from Ca2+ transport. J. Biol. Chem. 290, 14057–14067. doi: 10.1074/jbc.M115.636738
Sahoo, S. K., Shaikh, S. A., Sopariwala, D. H., Bal, N. C., and Periasamy, M. (2013). Sarcolipin protein interaction with sarco (endo) plasmic reticulum Ca2+ ATPase (SERCA) is distinct from phospholamban protein, and only sarcolipin can promote uncoupling of the SERCA pump. J. Biol. Chem. 288, 6881–6889. doi: 10.1074/jbc.M112.436915
Silva, J. E. (2011). Physiological importance and control of non-shivering facultative thermogenesis. Front. Biosci. 3, 352–371. doi: 10.2741/s156
Smith, W. S., Broadbridge, R., and East, J. M. (2002). Sarcolipin uncouples hydrolysis of ATP from accumulation of Ca2+ by the Ca2+-ATPase of skeletal-muscle sarcoplasmic reticulum. Biochem. J. 361, 277–286. doi: 10.1042/bj3610277
Toyoshima, C., and Inesi, G. (2004). Structural basis of ion pumping by Ca2+-ATPase of the sarcoplasmic reticulum. Annu. Rev. Biochem. 73, 269–292. doi: 10.1146/annurev.biochem.73.011303.073700
Keywords: sarcolipin, thermogenesis, evolution, muscle, calcium, SERCA
Citation: Campbell KL and Dicke AA (2018) Sarcolipin Makes Heat, but Is It Adaptive Thermogenesis? Front. Physiol. 9:714. doi: 10.3389/fphys.2018.00714
Received: 27 February 2018; Accepted: 24 May 2018;
Published: 14 June 2018.
Edited by:
Elias T. Polymeropoulos, Institute for Marine and Antarctic Studies (IMAS), AustraliaReviewed by:
Barbara Cannon, Stockholm University, SwedenCopyright © 2018 Campbell and Dicke. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Kevin L. Campbell, kevin.campbell@umanitoba.ca