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CORRECTION article

Front. Mol. Neurosci., 24 April 2019
Sec. Methods and Model Organisms
Volume 12 - 2019 | https://doi.org/10.3389/fnmol.2019.00103
This article is part of the Research Topic Biological and Therapeutic Roles of Glycine Receptors View all 16 articles

Corrigendum: A Novel RNA Editing Sensor Tool and a Specific Agonist Determine Neuronal Protein Expression of RNA-Edited Glycine Receptors and Identify a Genomic APOBEC1 Dimorphism as a New Genetic Risk Factor of Epilepsy

This article is a correction to:

  1. A Novel RNA Editing Sensor Tool and a Specific Agonist Determine Neuronal Protein Expression of RNA-Edited Glycine Receptors and Identify a Genomic APOBEC1 Dimorphism as a New Genetic Risk Factor of Epilepsy

    1. Read original article
\r\nSvenja KankowskiSvenja Kankowski1Benjamin FrsteraBenjamin Förstera2Aline WinkelmannAline Winkelmann3Pina KnauffPina Knauff4Erich E. WankerErich E. Wanker3Xintian A. YouXintian A. You5Marcus SemtnerMarcus Semtner6Florian Hetsch*Florian Hetsch1*Jochen C. Meier*Jochen C. Meier1*
  • 1Division Cell Physiology, Zoological Institute, Technische Universität Braunschweig, Braunschweig, Germany
  • 2Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilians University of Munich, Munich, Germany
  • 3Neuroproteomics, Max Delbrueck Center for Molecular Medicine, Berlin, Germany
  • 4Institute of Cell Biology and Neurobiology, Charité Universitätsmedizin Berlin, Berlin, Germany
  • 5Bioinformatics in Medicine, Zuse Institute Berlin, Berlin, Germany
  • 6Cellular Neurosciences, Max Delbrueck Center for Molecular Medicine, Berlin, Germany

A Corrigendum on
A Novel RNA Editing Sensor Tool and a Specific Agonist Determine Neuronal Protein Expression of RNA-Edited Glycine Receptors and Identify a Genomic APOBEC1 Dimorphism as a New Genetic Risk Factor of Epilepsy

by Kankowski, S., Förstera, B., Winkelmann, A., Knauff, P., Wanker, E. E., You, X. A., et al. (2018). Front. Mol. Neurosci. 10:439. doi: 10.3389/fnmol.2017.00439

In the original article, there was an error. The incorrect oligonucleotide sequences were provided.

A correction has been made to the Methods, subsection PCR-RFLP Analysis of Human TLE Samples:

“Resected hippocampal tissue of human iTLE patients (Eichler et al., 2008) was analyzed with regard to APOBEC1 gene dimorphism coding for 80M or 80I Apobec-1 protein variants. For this purpose, we developed a new PCR-based RFLP approach. Total RNA was isolated and reverse transcribed into cDNA as described earlier (Raltschev et al., 2016). Pre-amplification of Apobec-1 was performed using oligonucleotides 5′-CTTCAACCGGTGACCCCACTC-3′ and 5′-TGCGTACAACATCATCCACAGAGG-3′. Then, 3.5 μl of the pre-PCR were investigated in another PCR using oligonucleotides 5′-GAGTTTGACGTCTTCTATGACCC-3′ and 5′-GTTGACAAAATTCCTCCAGCAG-3′ to amplify a region spanning the 80M/I-coding position. This nested PCR amplification step yielded sufficient amount of DNA that was purified with Monarch® DNA Gel Extraction Kit (catalog no. L1020L, New England Biolabs GmbH) and digested using NlaIII restriction enzyme. NlaIII cuts at the 80M-coding position (CATG), and restriction fragments were separated using electrophoresis with 5% agarose gels to identify the genotype of the iTLE patients. For control purpose, Apobec-1 80I- or 80M-coding vectors for transfection were processed in parallel. Ethidium bromide was used to stain DNA bands.”

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

References

Eichler, S. A., Kirischuk, S., Jüttner, R., Schafermeier, P. K., Legendre, P., Lehmann, T. N., et al. (2008). Glycinergic tonic inhibition of hippocampal neurons with depolarising GABAergic transmission elicits histopathological signs of temporal lobe epilepsy. J. Cell. Mol. Med. 12, 2848–2866. doi: 10.1111/j.1582-4934.2008.00357.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Raltschev, C., Hetsch, F., Winkelmann, A., Meier, J. C., and Semtner, M. (2016). Electrophysiological signature of homomeric and heteromeric glycine receptor channels. J. Biol. Chem. 291, 18030–18040. doi: 10.1074/jbc.M116.735084

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: glycine receptors, epilepsy, temporal lobe, RNA editing, hippocampus, ligands

Citation: Kankowski S, Förstera B, Winkelmann A, Knauff P, Wanker EE, You XA, Semtner M, Hetsch F and Meier JC (2019) Corrigendum: A Novel RNA Editing Sensor Tool and a Specific Agonist Determine Neuronal Protein Expression of RNA-Edited Glycine Receptors and Identify a Genomic APOBEC1 Dimorphism as a New Genetic Risk Factor of Epilepsy. Front. Mol. Neurosci. 12:103. doi: 10.3389/fnmol.2019.00103

Received: 26 March 2019; Accepted: 04 April 2019;
Published: 24 April 2019.

Edited and reviewed by: Carmen Villmann, University of Wuerzburg, Germany

Copyright © 2019 Kankowski, Förstera, Winkelmann, Knauff, Wanker, You, Semtner, Hetsch and Meier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Florian Hetsch, f.hetsch@tu-braunschweig.de
Jochen C. Meier, jochen.meier@tu-braunschweig.de

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.