Thomas T. Liu
Maryam Falahpour- 1Center for Functional MRI, University of California, San Diego, La Jolla, CA, United States
- 2Departments of Radiology, Psychiatry, and Bioengineering, University of California, San Diego, La Jolla, CA, United States
Measures of resting-state functional magnetic resonance imaging (rsfMRI) activity have been shown to be sensitive to cognitive function and disease state. However, there is growing evidence that variations in vigilance can lead to pronounced and spatially widespread differences in resting-state brain activity. Unless properly accounted for, differences in vigilance can give rise to changes in resting-state activity that can be misinterpreted as primary cognitive or disease-related effects. In this paper, we examine in detail the link between vigilance and rsfMRI measures, such as signal variance and functional connectivity. We consider how state changes due to factors such as caffeine and sleep deprivation affect both vigilance and rsfMRI measures and review emerging approaches and methodological challenges for the estimation and interpretation of vigilance effects.
1. Introduction
Resting-state fMRI (rsfMRI) is a widely used method to characterize the functional organization of the brain at rest. A commonly used rsfMRI measure is the correlation coefficient between the blood oxygenation level dependent (BOLD) time series observed in different brain regions. This measure of functional connectivity (FC) has been shown to be sensitive to cognitive function and disease state (Greicius, 2008; Hampson et al., 2010). Other rsfMRI measures may also have diagnostic potential, such as the use of the variance of the rsfMRI global brain signal (defined as the mean of all BOLD signals in the brain) to distinguish schizophrenic patients from healthy controls (Yang et al., 2014). Because they do not require the subject to perform a task, rsfMRI measures are attractive for both research and clinical applications.
Despite the widespread use of rsfMRI-based methods, the origins of the underlying signals are still not well understood. However, there is growing evidence that fluctuations in vigilance can have a profound effect on the rsfMRI signal and derived metrics. In contrast to task-based fMRI studies in which there is an explicit task, rsfMRI studies are especially prone to vigilance effects due to the absence of an engaging task. Subjects often report difficulty in maintaining a constant level of vigilance or wakefulness during resting state scans. In a study that used data from over 1,100 subjects scanned by research groups across the world, Tagliazucchi and Laufs (2014) reported that about a third of participants lost wakefulness within the first 3 min of a resting-state scan and that half of the participants lost wakefulness after 10 min. In addition to vigilance fluctuations within a scan, there can be pronounced differences in the mean vigilance levels between subjects and scans, due to factors such as medication use, disease state, and anxiety levels. Unless properly accounted for, differences in vigilance can give rise to changes in resting-state activity that can be misinterpreted as primary disease-related effects. Most rsfMRI studies currently make the implicit assumption that all participants are in similar states of wakefulness or vigilance, but the validity of this assumption is rarely evaluated. A better understanding of potential vigilance effects is critical to the correct interpretation of both past and future rsfMRI studies.
Our goal in this paper is to critically review the relevant findings regarding the link between vigilance and the rsfMRI signal. We will examine the evidence relating variations in vigilance to the amplitude of the rsfMRI signal and derived metrics such as measures of both static and dynamic functional connectivity. We will also consider how state changes due to factors such as caffeine and sleep deprivation affects both vigilance and rsfMRI measures. Finally, we will review emerging methods for the estimation of vigilance effects and conclude with a consideration of methodological concerns, potential mechanisms, and future avenues of research.
2. Vigilance Metrics
In this work, we will primarily use the term vigilance but will also use related terms such as arousal and wakefulness. The term vigilance has been employed in a number of prior studies (Matejcek, 1982; Jobert et al., 1994; Oken et al., 2006; Olbrich et al., 2009). Additional related terms in the literature include cortical arousal, sustained attention, and tonic alertness (Oken et al., 2006; Sadaghiani et al., 2010; Olbrich et al., 2011).
In considering vigilance effects in rsfMRI studies, we will find it useful to consider independent measures of vigilance that are applicable to the resting-state. Most metrics of resting-state vigilance are based on EEG measures that have emerged from a wide range of scientific studies over the past century (Oken et al., 2006; Olbrich et al., 2009; Knaut et al., 2019). Other measures include pupilometry and percent eyelid closure. However, these metrics are only applicable to studies in which the subjects are instructed to keep their eyes open.
2.1. EEG-Based Metrics
For differentiating wakefulness from sleep and characterizing different sleep stages, EEG-based metrics have been standardized by the American Academy of Sleep Medicine (AASM, 2009), with a sleep stage score assigned to each 30 s epoch. In contrast, there is not currently a standard metric for characterizing the temporal fluctuations in arousal and vigilance that occur between wakefulness (W) and the first stage (N1) of non-REM sleep. Table 1 summarizes various EEG-based metrics that have been proposed over the past several decades. Although they differ in specific details, the metrics are generally related to the ratio of the power in middle frequency bands (e.g., α and β bands) associated with increased wakefulness to the power in lower frequency bands (e.g., δ and θ) associated with decreased wakefulness (Klimesch, 1999; Oken et al., 2006). In contrast to the 30 s epochs used for the standard sleep stage scores, these metrics have been used with temporal intervals as short as 1.8 s. In Jobert et al. (1994), Larson-Prior et al. (2009), and Wong et al. (2013), the proposed metrics have the form of either the ratio of the power in the alpha band to the power in the delta and theta bands or the square root of this ratio. Horovitz et al. (2008) used the inverse of the square root of the ratio as an inverse index of wakefulness. Olbrich et al. (2009) used the ratio of the power in the alpha band to the power in the delta, theta, and alpha bands. More recently, Knaut et al. (2019) proposed an EEG wakefulness index that is a ratio of powers that depends on both the EEG frequency band and topography. Table 1 also includes two related metrics recently utilized by Chang et al. (2016) for non-human primate studies.
Table 1. Vigilance metrics.
2.2. Other Metrics
In rsfMRI studies where subjects are instructed to keep their eyes open, measures of pupil or eyelid closure can be used to assess vigilance and arousal levels. For example a number of studies have used measures of eye closure to assess drowsiness and the presence of microsleeps (Poudel et al., 2014; Chang et al., 2016; Wang et al., 2016). Similarly, pupil diameter has been used to assess vigilance states during resting-state scans (Yellin et al., 2015; Schneider et al., 2016; Breeden et al., 2017).
3. Resting-State BOLD Signal Amplitude and Vigilance
In considering the relationship between BOLD signal amplitude and vigilance, investigators have considered (1) the mean amplitude of the BOLD signal in different brain regions of interest and (2) the amplitude of the global mean signal, defined as the average of the BOLD signals in either the entire brain or gray matter regions. Note that for rsfMRI signals the amplitude was defined in early studies as the standard deviation of the time course of interest (Fukunaga et al., 2006; Horovitz et al., 2008), a definition that has been adopted by a number of subsequent studies (Wong et al., 2012, 2013; Cordani et al., 2018). This is in contrast to the definition used in task-based fMRI for which the term amplitude typically refers to the difference between the BOLD signals measured in baseline and activation states. In addition to the use of the standard deviation, other metrics that are related to the amplitude have been used, including the variance of the rsfMRI signal (Jao et al., 2013; Yang et al., 2014) and the spectral power of the rsfMRI signal in a specified frequency band (Kiviniemi et al., 2005; Larson-Prior et al., 2009; Cordani et al., 2018). On the other hand, some studies have regressed the rsfMRI signal onto measures of vigilance state and examined the amplitudes of the regression fit coefficients as a function of state (Olbrich et al., 2009; Poudel et al., 2018). For these studies, the amplitudes can be interpreted as in a task-based fMRI study, with the fit coefficients providing information about the difference in BOLD signals between vigilance states.
3.1. Wakefulness to Light Sleep
The use of long duration (e.g., 30 min) resting-state scans has facilitated the study of the rsfMRI signal as subjects fluctuate between wakefulness and light sleep. In general, these studies have found that the amplitude of the BOLD signal increases with decreases in wakefulness. Fukunaga et al. (2006) reported that the mean BOLD signal amplitude in the visual cortex increased during early sleep stages, with amplitudes that were comparable to those observed with visual stimulation. In a follow-up study using simultaneous EEG-fMRI, Horovitz et al. (2008) confirmed the prior findings of Fukunaga et al. (2006) and further demonstrated a significant correlation between the BOLD signal amplitude and an Inverse Index of Wakefulness (see Table 1) in multiple brain regions, including the visual cortex, auditory cortex, and precuneus. Larson-Prior et al. (2009) found that the global signal spectral power significantly increased during light sleep as compared with awake states, with a general trend toward significance in individual regions of interest. Using a measure of vigilance stages, Olbrich et al. (2009) found that decreases in vigilance were associated with an increase in the BOLD signal amplitude in the occipital cortex, the anterior cingulate, the frontal cortex, the parietal cortices, and the temporal cortices and a decrease in BOLD signal amplitude in the thalamus and frontal regions. McAvoy et al. (2018) demonstrated that the amplitude of the global mean signal increased with sleep depth. They concluded that the increase in global signal amplitude reflected a proportionally greater decrease in oxygen consumption with sleep as compared to the sleep-related decrease in blood flow.
3.2. Variations Across Subject Scans and States
The relation between BOLD signal amplitude and vigilance can also be examined by considering variations in the two quantities across scans and experimental conditions. Wong et al. (2013) looked at the amplitude of the resting-state global signal and EEG vigilance measures across scans and found a strong and significant negative correlation between the two quantities when subjects were studied in the eyes-closed condition, with a weaker and nearly significant correlation observed in the eyes-open condition. Thus, scans for which the subjects exhibited relatively higher vigilance levels had lower global signal amplitudes, while scans with relatively lower vigilance levels were associated with higher global signal amplitudes.
Wong et al. (2013) also considered the effects of caffeine on vigilance and global signal amplitude and found that increases in vigilance due to caffeine were significantly correlated with decreases in the amplitude of the resting-state global signal. In contrast, in a study using the sedative midazolam, Kiviniemi et al. (2005) found an increase in the spectral power of low frequency BOLD fluctuations. Similarly, Esposito et al. (2010) found that the depressant alcohol increased spontaneous BOLD fluctuations in the visual cortex. These pharmacological studies further support the notion of an inverse relation between vigilance and the amplitude of resting-state BOLD fluctuations.
A number of studies have examined differences in resting-state fMRI activity between the eyes-closed (EC) and eyes-open (EO) conditions (Yang et al., 2007; McAvoy et al., 2008, 2012; Bianciardi et al., 2009; Yan et al., 2009; Zou et al., 2009; Jao et al., 2013; Patriat et al., 2013; Xu et al., 2014; Yuan et al., 2014). In general, these studies have found that the amplitude of the resting-state BOLD signal is decreased in the eyes-open condition as compared to the eyes-closed condition. For example, Jao et al. (2013) found that the average variance of the BOLD signal was significantly lower in the eyes-open condition. There is some diversity in the findings, with regional resting-state activity sometimes found to be higher in the EO condition, with the differences most likely reflecting variations in processing approaches, such as the use of global signal regression and physiological noise reduction in some studies and not others.
Using simultaneous EEG fMRI, Wong et al. (2015) demonstrated an overall increase in EEG vigilance in the EO state as compared to the EC state and found that these increases in vigilance were negatively correlated with the differences in global signal amplitude between the two states. Interestingly, the slope between the changes in vigilance and differences in global signal amplitude was similar to the slope found in Wong et al. (2013) relating the caffeine-induced changes in vigilance and global signal amplitude. The similarity between the relationships observed for the EO-EC changes and the caffeine-related changes suggest that the basic mechanisms underlying the vigilance and global signal amplitude relationship may to some extent be independent of the experimental manipulation.
Cordani et al. (2018) found that the resting-state BOLD signal amplitude in the sensory cortices decreases at times corresponding to dawn and dusk, possibly reflecting an anticipatory mechanism in which spontaneous activity is reduced in order to improve visual perception during times associated with low light levels. Yeo et al. (2015) reported that the amplitude of the global signal increased with sleep deprivation. Similarly, Poudel et al. (2018) observed spatially widespread increases in the BOLD signal associated with microsleeps after both normal rest and sleep deprivation conditions.
4. Temporal Fluctuations in Vigilance During a Scan
The various studies reviewed in the prior section largely support the overall conclusion that the amplitude of the resting-state BOLD signal over a given time period is inversely proportional to the average vigilance level of the subject during that period. Over the course of scan, there are also moment to moment variations in arousal and vigilance. Their effect on rsfMRI data can be assessed by looking at the correlation between the rsfMRI signal and a vigilance-associated time course. Using simultaneous EEG fMRI, a number of studies have established that EEG alpha power is negatively correlated with fMRI signals in widespread regions of the brain, including the visual and fronto-parietal cortices (Goldman et al., 2002; Laufs et al., 2003a,b; Moosmann et al., 2003). Positive correlations have been reported for the thalamus, insula, and anterior cingulate (Goldman et al., 2002; Moosmann et al., 2003; Feige et al., 2005; Difrancesco et al., 2008; Sadaghiani et al., 2010). Using the metric of EEG vigilance from Wong et al. (2013), Falahpour et al. (2018a) found similar spatial patterns of correlation, which is expected given the close link between the vigilance metric and alpha power.
It has also been shown that there is a significant negative correlation between the rsfMRI global signal and EEG vigilance time courses (Falahpour et al., 2016, 2018a). Figure 1 shows an example of this negative correlation, where the plot in the middle row shows the vigilance time series in blue and the global signal (inverted for display) in red. The top row shows the rsfMRI images obtained by averaging over time points corresponding to the top 10% of vigilance values. Consistent with prior observations, these show positive signal values in the thalamus and negative values in sensory areas. In the bottom row, images obtained by averaging over time points with the lowest 10% of vigilance values shows the opposite pattern, with negative signal values in the thalamus and positive values in sensory areas.
Figure 1. Patterns associated with high and low vigilance and relation between vigilance and the global signal. (Top) Average rsfMRI image from the time points corresponding to the top 10% of vigilance values. (Middle) Vigilance time course in blue and the global signal (inverted for display) in red, with a correlation of r = −0.33. (Bottom) Average rsfMRI image from the time points corresponding to the lowest 10% of vigilance values.
Han et al. (2019) have recently put forth the hypothesis that the observed correlations between the EEG and rsfMRI signals are due to stereotypical electrophysiological events, first observed in the global signal of large-scale electrocorticography (ECoG) recordings from monkeys by Liu et al. (2015). These sequential spectral transition (SST) events were found to last for 10–20 s and consisted of a decrease in mid-band (alpha and beta; 8–30 Hz) activity followed by an increase in low frequency (delta and theta; < 30 Hz) activity and a burst of high-frequency broadband gamma activity (>30 Hz). The SST events were later shown to be coupled with peaks in the rsfMRI global signal (Liu et al., 2018), roughly consistent with the aforementioned findings of a significant negative correlation between the rsfMRI global signal and EEG vigilance time series. As further evidence for the role of transient activity in EEG-BOLD correlations, Poudel et al. (2014) observed transient changes in BOLD activity associated with microsleeps. Furthermore, in subjects who exhibited a higher occurrence of microsleeps, the authors found that post-central EEG theta power was positively correlated with the BOLD signal in the thalamus, basal forebrain, visual, posterior parietal, and prefrontal cortices.
Using pupilometry, Schneider et al. (2016) found that spontaneous pupil dilations were associated with increased BOLD activity in the salience network, thalamus, and frontoparietal regions, whereas spontaneous pupil constrictions were associated with increased BOLD activity in the visual and sensoriomotor areas. Similarly, several studies have reported a positive correlation between pupil size and the rsfMRI BOLD signal in regions comprising cingulo-opercular, default mode, and fronto-parietal networks and a negative correlation in the visual and sensorimotor regions (Yellin et al., 2015; Breeden et al., 2017; DiNuzzo et al., 2019). To first order, the spatial pattern of correlations observed with pupilometry are roughly consistent with those reported using simultaneous EEG fMRI studies. In addition, a positive correlation has been demonstrated between pupil size and BOLD activity in the locus coeruleus (Murphy et al., 2014; DiNuzzo et al., 2019), a nucleus in the brainstem that contains norepinephrine neurons that are thought to modulate pupil size (Joshi et al., 2016).
5. Functional Connectivity and Vigilance
Complementing the work relating vigilance to BOLD amplitude, the connection between vigilance and rsfMRI FC has also been explored by a number of investigators. The main investigative approaches include examining (1) differences in FC across sleep stages, (2) temporal variations in FC as a function of alpha power or a related time-varying measure of vigilance, and (3) FC changes associated with induced changes in state. A recent extensive review of the relation between FC and sleep has been provided by Tagliazucchi and Van Someren (2017). The effects of sleep deprivation have been recently reviewed by Chee and Zhou (2019).
5.1. Functional Connectivity and Sleep Stages
Both Larson-Prior et al. (2011) and Sämann et al. (2011) reported decreases in the extent of anti-correlations between the default mode network (DMN) and the task positive network (TPN) during the transition to light sleep. Tagliazucchi et al. (2012a) demonstrated that FC measures and nonlinear support vector machines could be used to classify sleep stages and later used this relation to characterize wakefulness levels across a large collection of rsfMRI studies (Tagliazucchi and Laufs, 2014). Subsequently, Altmann et al. (2016) found that linear support vector machines could also be used to predict sleep stages from FC measures.
Haimovici et al. (2017) found that dynamic functional connectivity states obtained through clustering algorithms were similar to the average FC state found in each sleep stage, suggesting that variations in dynamic functional connectivity states are associated with fluctuations in wakefulness. Zou et al. (2019) later reported similar findings. Using a Hidden Markov model (HMM) approach, Stevner et al. (2019) identified multiple FC states associated with each sleep stage and characterized the transition between states within and between stages. Laumann et al. (2017) showed that a multivariate measure of kurtosis was significantly correlated with an index of sleep and argued that this was evidence for a sleep-related increase in the temporal variability of FC measures.
5.2. Alpha Power and FC
In examining dynamic fluctuations in FC across the duration of a scan, Tagliazucchi et al. (2012b) found that time-varying increases in alpha power were correlated with decreases in functional connectivity as measured in awake subjects. As increased alpha power is proportional to vigilance (see EEG metrics), this finding suggests that time-varying FC decreases with increased vigilance in a manner that is largely consistent with the reductions in FC observed across an entire scan when mean vigilance increases (e.g., with caffeine). Similarly, Scheeringa et al. (2012) reported that increases in alpha power were associated with a decrease of FC within the visual system and also a diminishing of the negative relation between the visual cortex and thalamus. In related work, Chang et al. (2013) observed that the time-varying strength of connectivity between the DMN and default attention network (DAN) was inversely proportional to the alpha power measured within the same time window (40 s window length).
Allen et al. (2018) noted that certain dynamic FC (DFC) states were found more frequently in the EO condition while other DFC states were found predominantly in the EC condition. They identified a DFC state related to increased drowsiness (lower alpha and higher delta and theta power) in which there were high levels of FC in the sensorimotor and visual regions and the increased presence of anti-correlations between the thalamus and these regions.
5.3. Induced State Changes
Caffeine has been shown to lead to spatially widespread decreases in rsfMRI FC measures (Wong et al., 2012). Using the same sample of subjects, Tal et al. (2013) employed source-localized magnetoencephalography (MEG) to demonstrate similar decreases in MEG-based measures of resting-state connectivity. In a follow-up study, Wong et al. (2013) reported that caffeine-induced increases in EEG vigilance were significantly correlated with increases in the anti-correlation between nodes of the DMN and TPN. Taking into account the observation that caffeine also reduces the amplitude of the global signal, the authors concluded that the increased presence of anti-correlations could be largely attributed to the reduction in global rsfMRI activity (Wong et al., 2012, 2013). In addition, the caffeine-induced increases in anti-correlation were consistent with the aforementioned decreases in anti-correlations observed in the transition to light sleep (Larson-Prior et al., 2011; Sämann et al., 2011).
In studies with the sedative midazolam, Kiviniemi et al. (2005) found increased FC within the sensory-motor network, consistent with the observed increase in the amplitude of BOLD signal fluctuations. Further evidence for this increase was presented by Greicius et al. (2008), who observed a midazolam-related increase in FC in the sensory-motor network, but reported a decrease in FC in the DMN. In a study using the sedative zolpidem, Licata et al. (2013) reported a drug-related increase in FC in a number of sensory, motor, and limbic networks.
As discussed in section 3.2, vigilance is higher and the global signal amplitude is lower in the EO versus the EC state. It has also been found that functional connectivity is generally lower in the EO state as compared to the EC state (McAvoy et al., 2008; Bianciardi et al., 2009; Zou et al., 2009; Xu et al., 2014). This decreased connectivity is consistent with the decreased global activity and increased vigilance in the EO state. Furthermore, these decreases in global signal amplitude and increases in vigilance may account for the increased reliability of connectivity measures obtained in the EO condition as compared to the EC state (Patriat et al., 2013).
In reviewing prior studies that have observed state-based changes in functional connectivity, it is important to note that there can be some variability in the reporting of connectivity changes, especially when there are both positive and negative correlation values. As an example, for the studies reporting reduced connectivity in the EO state as compared to the EC state, the findings can be divided into three groups: (1) both EO and EC correlation values are positive, and the EO values are less positive (i.e., smaller numerical value) (McAvoy et al., 2008; Bianciardi et al., 2009), (2) the EO and EC correlation values are either both positive or both negative, and the EO values are either less positive or less negative, respectively (i.e., absolute magnitude of the EO correlation values are smaller independent of the sign) (Zou et al., 2009), or (3) EC values are positive, and EO values are either less positive or negative (i.e., the EC values are greater than the EO values, with the possibility that a negative EO value could have a larger magnitude than a positive EC value) (Xu et al., 2014). Paying attention to the sign of the correlation values is especially important when examining studies that use global signal regression, since this preprocessing step has been shown to introduce negative correlation values (Murphy et al., 2009).
5.4. Sleep Deprivation
De Havas et al. (2012) found that sleep deprivation led to reductions in both DMN functional connectivity and the degree of anticorrelation between the DMN and other regions. These findings were supported by a follow-up study by Yeo et al. (2015), who reported that subjects who exhibited less vigilance declines after sleep deprivation showed stronger anti-correlations among several networks. These results were obtained with global signal regression. When GSR was not applied, Yeo et al. (2015) observed a spatially widespread increase in functional connectivity with sleep deprivation. Wirsich et al. (2018) also reported widespread increases in FC with sleep deprivation. Zhang et al. (2019) found that sleep deprivation lead to decreases in FC between the cerebellum and a number of brain regions and an increase in FC between the cerebellum and bilateral caudate.
Ong et al. (2015) examined spontaneous eye closures in sleep deprived subjects and reported additional reductions in the FC in the DMN and DAN beyond what had been previously observed for sleep deprivation. In a related study, Wang et al. (2016) went on to identify a low arousal DFC state associated with spontaneous eye closures and another high arousal state associated with periods of the eyes remaining wide open. Patanaik et al. (2018) found that subjects with a greater fraction of high arousal states showed higher levels of vigilance, working memory, and processing speed after sleep restriction.
Kaufmann et al. (2016) reported that sleep deprivation led to significant alterations in several resting-state FC networks, including the dorsal attention, default mode, and hippocampal networks, with an overall increase in FC values with sleep deprivation. Furthermore, they found differences in FC between morning and evening measures with a return to morning FC patterns after a night of sleep. They used partial correlation instead of global signal regression. Tüshaus et al. (2017) observed that sleep pressure led to significant changes in the FC between resting-state networks as determined using independent components analysis. Yang et al. (2018) reported that sleep deprivation led to decreases in FC density (FCD) in brain regions including the posterior cingulate cortex and precuneus and increases in sensory integration and arousal regulating areas, such as thalamus.
6. fMRI-Based Vigilance Estimates
As discussed above, EEG and measures of eye closure or pupil size can be used to assess vigilance during rsfMRI studies. However, the additional acquisition and analysis efforts associated with these measures have precluded their widespread use in rsfMRI studies. Simultaneous EEG-fMRI scans are technically challenging and require specialized equipment and substantial set-up time (with current technologies). Pupilometry poses less of a logistical challenge, but the equipment costs, set-up time, and analysis requirements can still complicate its adoption for many studies. Video-based measures of eye closure are more readily implemented and demand relatively little setup-time, but still require additional effort to analyze the images. Nevertheless, more rsfMRI studies should probably consider regular video monitoring of eye state given the relative ease of use and the potential benefit of the information.
Vigilance estimates based on the fMRI data alone can serve as a useful alternative to external measures, especially in studies where there is limited time available for additional set-up procedures. As noted in prior sections, Tagliazucchi et al. (2012a) and Altmann et al. (2016) used windowed rsfMRI connectivity estimates to estimate sleep stages in 30 s epochs. To provide estimates on a finer time scale, Chang et al. (2016) introduced an fMRI template-based approach to estimate arousal fluctuations in awake monkeys sitting in complete darkness. In this approach, a spatial template was first formed, where the value of each voxel in the template reflected the strength of the correlation between the fMRI data and an eye-based metric of arousal. Correlation of this spatial template with each volume of an independent fMRI dataset was then used to form an estimate of arousal for each timepoint in the test dataset.
Falahpour et al. (2018a) subsequently applied the template-based approach to simultaneous EEG-fMRI data acquired in humans and demonstrated the ability to predict EEG-based measures of vigilance fluctuations, supporting the generalizability of the approach from macaques to humans. They also demonstrated that the performance of the method was related to the overall amount of variability in a subject's vigilance state and that the approach could be used to estimate the variability across scans in the amplitude of the vigilance fluctuations. In a recent preprint, Gu et al. (2019) used a global co-activation map (Liu et al., 2018) as a template and found that the resulting estimates were similar to those derived using the template in Falahpour et al. (2018a). A graphical summary of the template-based approach is provided in Figure 2.
Figure 2. Graphical summary of the template-based approach for prediction of vigilance fluctuations as described in Chang et al. (2016). The vigilance template is obtained by correlating the fMRI data with an estimate of EEG vigilance measure as described in Falahpour et al. (2018a), using data originally acquired for a prior study (Wong et al., 2013). This vigilance template was then applied to an independent simultaneous EEG-fMRI dataset. For each timepoint in the fMRI data, the spatial correlation between the template and the fMRI volume is computed to form an estimate of vigilance (red line). This estimate is highly correlated (r = 0.51) with the EEG-based measure of vigilance (blue line).
7. Methodological Considerations
One of the challenges in understanding vigilance effects in rsfMRI stems from the presence of noise components (both BOLD and non-BOLD weighted) such as system-related instabilities, subject motion, and physiological fluctuations. While there have been considerable efforts to characterize and mitigate the effects of these components in BOLD fMRI time series (Birn, 2012; Greve et al., 2013; Murphy et al., 2013; Power et al., 2015), the choice of methods varies widely between rsfMRI studies. This lack of uniformity makes it difficult to compare results across studies. For studies of vigilance, the problem is further complicated by the connection between vigilance and several of the primary noise confounds, such as the global signal, motion, and respiratory activity (Yuan et al., 2013; Liu et al., 2017; Patanaik et al., 2018). For example, Yuan et al. (2013) reported that the correlation between EEG alpha power and rsfMRI signal was reduced after respiratory and cardiac nuisance regressors were projected out of the rsfMRI data. Similarly, Patanaik et al. (2018) found that the relation between vigilance and the global signal was reduced when motion was used as a covariate.
In the case of the global signal, there is still an ongoing debate as to whether the global signal should be regressed out prior to the analysis of rsfMRI data (Liu et al., 2017). Due to the relation between the global signal and vigilance, the use of global signal regression (GSR) can have a significant effect on findings regarding the connection between vigilance and the rsfMRI signal. As an example, Falahpour et al. (2018b) noted that prior studies that did not use GSR generally found a negative correlation between EEG alpha power (or vigilance) and the BOLD signal in widespread regions of the brain, including the lingual gyrus, posterior cingulate, cuneus, and precuneus (Goldman et al., 2002; Laufs et al., 2003b; Falahpour et al., 2018a). In contrast, a study that used GSR found positive correlations in additional areas not reported in prior studies, including the dorsal anterior cingulate cortex, the anterior insula, and the anterior prefrontal cortex (Sadaghiani et al., 2010). Falahpour et al. (2018b) went on to demonstrate that GSR induced a positive shift in the correlation between EEG vigilance and the rsfMRI signal, roughly consistent with the discrepancy in the prior findings.
To address the methodological challenges, the support and use of open multimodal neuroimaging databases and standardized processing approaches (Poldrack et al., 2017; Babayan et al., 2019) will become increasingly important. These resources will facilitate the comparison of various methods and studies and enable researchers to better understand the relation between rsfMRI and vigilance measures.
8. Vigilance and Disease
There is growing evidence that disregulation of arousal is associated with a variety of mental disorders such as depression, autism, and schizophrenia (Boutros et al., 2008; Razavi et al., 2013; Sander et al., 2016; Jawinski et al., 2019). In a genome-wise association study, Jawinski et al. (2019) found an association between resting-state vigilance levels (as assessed with EEG) and genetic markers for major depressive disorder, autism spectrum disorder, and Alzheimer's disease. In parallel, there has been widespread use of rsfMRI to study disease-related alterations in resting-state brain activity and connectivity. For example, rsfMRI studies of schizophrenia have reported disease-related differences in functional connectivity and signal variance (Calhoun et al., 2011; Yang et al., 2014; Wang et al., 2015). Given the link between rsfMRI measures and vigilance and prior findings indicating a decrease in EEG vigilance with schizophrenia (Boutros et al., 2008; Razavi et al., 2013), it is likely that disease-related vigilance effects contributed to the observed differences. Yang et al. (2014) reported that the variance of the global signal was significantly higher in patients with schizophrenia as compared to normal controls and concluded that the differences reflected an increase in neural coupling. However, the authors acknowledged that the potential confound of vigilance differences between groups would need to be carefully considered in follow-up work.
9. Potential Mechanisms
Although the mechanisms underlying the relationship between vigilance fluctuations and the rsfMRI signal are not well understood, the evidence from prior observational studies (see section 4) suggests a link with activity in brain regions related to arousal, such as the basal forebrain and the locus coeruleus. Recent studies involving invasive neuromodulation in animal models support this view. Turchi et al. (2018) observed reduced global signal fluctuations in macaques with inactivation of the nucleus basalis of Meynert, a group of neurons in the basal forebrain with widespread arousal-related modulatory projections to the cortex. Using chemogenetic activation of the locus coeruleus in a mouse model, Zerbi et al. (2019) found an increase in functional connectivity in several networks, including the salience network, consistent with the relation between increased rsfMRI activity and pupil size discussed in section 4.
As the rsfMRI signal is a complex reflection of neural, metabolic, and vascular factors (Liu, 2013), a better understanding of the link between vigilance and the rsfMRI signal requires deeper insight into the relative contribution of these factors. Using data from all-night EEG-fMRI sleep studies, Özbay et al. (2019) demonstrated a tight relationship between the occurrence of K-complexes, episodic drops in finger skin vascular tone, and widespread decreases in the rsfMRI signal. The authors argued that the findings were consistent with a picture in which increased sympathetic activity associated with K-complexes resulted in vasoconstriction of the cerebral vasculature and a concomitant decrease in the rsfMRI signal. As this study focused on activity during NREM Stage 2 sleep, additional studies will be needed to further elucidate the role of arousal-related sympathetic activity in rsfMRI studies during which the subjects are largely awake.
10. Conclusion
There is now substantial evidence indicating that vigilance effects play a significant role in resting-state fMRI studies. The first order effects are summarized in Figure 3. In general, there is a negative correlation between vigilance and global rsfMRI activity, with higher vigilance levels leading to global reductions in signal variance and functional connectivity and an increase in the presence of anti-correlations in functional connectivity maps. However, the details of the observed effects vary between studies and conditions. While part of this variation may reflect differences in processing and analysis approaches, it is likely that a significant part of the remaining variation reflects different underlying causes for the vigilance changes and variability. A better understanding of the mechanisms linking vigilance and resting-state brain activity will be helpful for understanding these variations and their impact on the interpretation of rsfMRI studies. Toward that end, invasive studies in animal models (Turchi et al., 2018; Zerbi et al., 2019) can provide insights not readily attainable in human studies. Finally, differences in vigilance can give rise to changes in resting-state activity that can be misinterpreted as primary disease-related effects. The further development of approaches to better estimate and account for vigilance effects will play a critical role in the improved interpretation of rsfMRI data in both clinical and research settings.
Figure 3. Overview of the relationship between vigilance and rsfMRI signal amplitude and functional connectivity. In general, vigilance is negatively correlated with rsfMRI signal amplitude, with higher vigilance levels corresponding to global reductions in fMRI activity and functional connectivity. These reductions are associated with a greater presence of anti-correlations in functional connectivity maps at higher vigilance levels. The functional connectivity maps were obtained using a seed signal from the posterior cingulate cortex and acquired before (left) and after (right) the administration of caffeine (Wong et al., 2012). The bottom plot shows representative EEG spectra for low, medium, and high vigilance levels (Wong et al., 2013).
Author Contributions
All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.
Funding
This work was supported in part by NIH grant R21MH112155.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: vigilance, arousal, wakefulness, fMRI, EEG, functional connectivity
Citation: Liu TT and Falahpour M (2020) Vigilance Effects in Resting-State fMRI. Front. Neurosci. 14:321. doi: 10.3389/fnins.2020.00321
Received: 08 November 2019; Accepted: 18 March 2020;
Published: 23 April 2020.
Edited by:
Shella Keilholz, Emory University, United StatesReviewed by:
Michael W. L. Chee, National University of Singapore, SingaporeEnzo Tagliazucchi, Goethe University Frankfurt, Germany
Copyright © 2020 Liu and Falahpour. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Thomas T. Liu, dHRsaXUmI3gwMDA0MDt1Y3NkLmVkdQ==