Giuseppantonio Maisetta*
Giovanna Batoni*- Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy
Editorial on the Research Topic
Interspecies Interactions: Effects on Virulence and Antimicrobial Susceptibility of Bacterial and Fungal Pathogens
One of the most exciting achievements that microbiologists have pursued over the last decades is the recognition that microorganisms rarely live as single fluctuating entities but strictly interact with each other in complex communities known as biofilms. Studies on dental biofilms, intestinal communities, chronic wounds, or respiratory infections in patients with cystic fibrosis clearly demonstrate that community interactions greatly influence microbial survival and disease progression (Dalton et al., 2011; Caverly et al., 2015; Reynolds et al., 2015; Marsh and Zaura, 2017). These interactions range from synergism to competition and involve, among others, physical interactions, chemical signaling, and exchange of genetic information. There is no doubt that consideration of the social behavior of microorganisms can reveal emergent traits and mechanisms of pathogenicity that would be overlooked by studying bacteria in isolation. For instance, community establishment provides members with additional properties such as enhanced tolerance to antimicrobials, ability to evade host immune responses or to survive in harmful environments (Batoni et al., 2016).
This Research Topic gathers 11 articles from 71 authors exploring different aspects of species-to-species interactions. We believe that a deep understanding of the mechanisms at the basis of the ecological interactions among microbial species will provide the knowledge needed to translate novel interventions for the diagnosis, treatment, and prevention of poly-microbial infections into the clinic, and hope that this Research Topic may contribute to this purpose.
Certainly, the study of interspecies interactions strongly relies on the availability of suitable experimental models that enable the stable and long-term cultivation of poly-microbial communities (Røder et al., 2020). In this respect, the Topic includes three studies aimed at reproducing the features of specific body sites and at exploring the multiple interactions among commensals and pathogenic organisms, as well as antimicrobials. O'Brien and Welch described a new continuous-flow model for in vitro cultivation of mixed bacteria associated with cystic fibrosis airway infections, while Jordana-Lluch et al. developed and validated a simple 2D skin infection model for investigating commensals, pathogens and keratinocytes interactions. Finally, by employing an in vitro nasopharyngeal colonization model that mimics the conditions of the human nasopharynx including temperature, nutrient availability, aeration, and epithelial attachment, Bair and Campagnani studied the co-colonization dynamics of three main othopatogens: Moraxella catarrhalis, non-typable Haemophilus influenzae (NTHi), and Streptococcus pneumoniae and found that the presence of M. catarrhalis is essential for NTHi to survive the bactericidal effects of S. pneumoniae.
One of the most obvious translational aspect of interspecies interactions is the use of “friend” microorganisms, the so-called probiotics, to prevent or cure diseases caused by microorganisms endowed high pathogenic potential. Although traditionally used to restore intestinal flora after prolonged antibiotic therapy, probiotics have been considered as means to prevent/treat a variety of diseases during the last decade (Sales-Campos et al., 2019). In this Research Topic, two papers concerning the employment of candidate probiotics against oral pathogens are included. Moman et al. demonstrated that bacterial strains such as Lactobacillus reuteri and Streptococcus salivarius decrease the toxic effects of the periodontal pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans toward oral keratinocytes and in an in vivo model of G. mellonella larvae. Zhang et al. reported that Lactobacillus plantarum K41 is able to inhibit biofilm formation of the highly cariogenic species Streptococcus mutans and observed a significant reduction in the incidence and severity of dental caries in rats pretreated with this probiotic strain.
Kang et al. showed that competitive interactions among microorganisms can be exploited to protect plants by phytopathogens. They studied how the gene expression of the pathogenic fungus Gaeumannomyces graminis var. tritici was affected by Bacillus velezensis, an endophytic biocontrol bacterium exhibiting a broad antifungal spectrum against many phytopathogens.
The close relationship between interspecies interactions and susceptibility to antimicrobials is a rapidly expanding research area that is likely to provide new clues to face the worrisome and world- spreading problem of antimicrobial resistance (AMR) (Radlinski and Conlon, 2018). In this direction, Banerji et al. present a comprehensive and interesting review addressing the relation between microbial interactions and AMR from an ecological point of view. Highlighting that human, animal, and environmental systems are strictly interconnected, the Authors show that species interactions may play significant and sometimes multifaceted roles in determining the prevalence and distribution of AMR and antimicrobial resistance-associated genes (ARGs).
The effects of interspecies interactions on antibiotic susceptibility are not limited to closely related microorganisms but can actually cross kingdom borders (Harriott and Noverr, 2009). Nabb et al. disclose an interesting mechanism by which C. albicans may promote multi-drug tolerance in S. aureus. They report that S. aureus grown in dual cultures with C. albicans displays decreased intracellular ATP concentrations as well as lower membrane potential when compared to cultures lacking C. albicans. Collectively, the data reported demonstrate that decreased metabolic activity through nutrient deprivation may induce the formation of persister cells and represent a mechanism for increased antibiotic tolerance within polymicrobial cultures.
Interestingly, three articles from the Research Topic highlight how interactions between species can not only negatively affect the susceptibility of microbial populations to antimicrobials, but also be exploited for the identification of new drugs or drug targets. In the first of these articles, Hardy et al. screened a number of clinical bacterial isolates obtained from a variety of body sites for the ability to inhibit multiple S. aureus strains. They found that the majority of the isolates inhibited at least one S. aureus strain including MRSA. Furthermore, many of the clinical isolates belonging to the Staphylococcus and Corynebacterium genera mediated contact-independent inhibitory or bactericidal activity against S. aureus warranting the characterization of the active entities at the molecular level to reveal novel S. aureus therapeutics. In the second article, Herbrik et al. studied a strain of Streptomyces (TR1341) isolated from the sputum of a tuberculosis patient. They demonstrated that TR1341 produces at least two bioactive compounds with fungicidal or antibacterial/anti-virulence activity. Finally, in the third article (Veerapandian and Vediyappan), an in vitro study was carried out demonstrating the inhibition of mono-species or dual-species biofilms of S. gordonii and C. albicans, by gymnemic acid (GAs), a non-toxic small molecule inhibitor of fungal hyphae. The study shows that S. gordonii stimulates the expression of adhesive materials in C. albicans by direct interaction and/or signaling, and that the adhesive material expression can be inhibited by GAs.
Overall, we believe that the articles collected in this Research Topic represent a step forward for a better understanding of microbe-microbe interactions and their effects on infection outcome and antibiotic susceptibility. We hope that this article collection may encourage further studies in this research field aimed to develop new preventive and/or therapeutic approaches against poly-microbial infections.
Author Contributions
GM and GB equally contributed to the writing of the manuscript. All authors contributed to the article and approved the submitted version.
Funding
This work was supported by institutional funds from the University of Pisa.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We thank Dr. Semih Esin for critical reading of the manuscript.
References
Batoni, G., Maisetta, G, and Esin, S. (2016). Antimicrobial peptides and their interaction with biofilms of medically relevant bacteria. Biochim. Biophys. Acta. 1858, 1044–1060. doi: 10.1016/j.bbamem.2015.10.013
Caverly, L. J., Zhao, J., and LiPuma, J. J. (2015). Cystic fibrosis lung microbiome: opportunities to reconsider management of airway infection. Pediatr. Pulmonol. 50(Suppl. 40):S31–S38. doi: 10.1002/ppul.23243
Dalton, T., Dowd, S. E., Wolcott, R. D., Sun, Y., Watters, C., Griswold, J. A., et al. (2011). An in vivo polymicrobial biofilm wound infection model to study interspecies interactions. PLoS ONE 6:e27317. doi: 10.1371/journal.pone.0027317
Harriott, M. M., and Noverr, M. C. (2009). Candida albicans and Staphylococcus aureus form polymicrobial biofilms: effects on antimicrobial resistance. Antimicrob. Agents Chemother. 53, 3914–3922. doi: 10.1128/AAC.00657-09.
Marsh, P. D., and Zaura, E. (2017). Dental biofilm: ecological interactions in health and disease. J. Clin. Periodontol. 44(Suppl. 18):S12–S22. doi: 10.1111/jcpe.12679
Radlinski, L., and Conlon, B. P. (2018). Antibiotic efficacy in the complex infection environment. Curr. Opin. Microbiol. 42, 19–24. doi: 10.1016/j.mib.2017.09.007
Reynolds, L. A., Finlay, B. B., and Maizels, R. M. (2015). Cohabitation in the intestine: interactions among helminth parasites, bacterial microbiota, and host immunity. J. Immunol. 195, 4059–4066. doi: 10.4049/jimmunol.1501432
Røder, H. L., Olsen, N. M. C., Whiteley, M., and Burmølle, M. (2020). Unravelling interspecies interactions across heterogeneities in complex biofilm communities. Environ. Microbiol. 22, 5–16. doi: 10.1111/1462-2920.14834
Keywords: interspecies interactions, polymicrobial infections, virulence, biofilm, susceptibility
Citation: Maisetta G and Batoni G (2020) Editorial: Interspecies Interactions: Effects on Virulence and Antimicrobial Susceptibility of Bacterial and Fungal Pathogens. Front. Microbiol. 11:1922. doi: 10.3389/fmicb.2020.01922
Received: 27 May 2020; Accepted: 21 July 2020;
Published: 26 August 2020.
Edited by:
Natalia V. Kirienko, Rice University, United StatesReviewed by:
Alexandra R. Lucas, Arizona State University, United StatesMilya Davlieva, Quantapore, United States
Copyright © 2020 Maisetta and Batoni. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Giuseppantonio Maisetta, giuseppantonio.maisetta@dps.unipi.it; Giovanna Batoni, giovanna.batoni@med.unipi.it