Corrigendum: Identification of a Novel Small RNA srvg23535 in Vibrio alginolyticus ZJ-T and Its Characterization With Phenotype MicroArray Technology

A Corrigendum on
Identification of a Novel Small RNA srvg23535 in Vibrio alginolyticus ZJ-T and Its Characterization With Phenotype MicroArray Technology

by Deng, Y., Su, Y., Liu, S., Guo, Z., Cheng, C., Ma, H., et al. (2018). Front. Microbiol. 9:2394. doi: 10.3389/fmicb.2018.02394

In the published article, there was an error in affiliation 1. Instead of “Ministry of Agriculture” the correct name of the ministry is “Ministry of Agriculture and Rural Affairs”.

In Table 1, the references for “53813,” “GEB88,” and “pSW7848,” were incorrectly written as “This lab.” It should be “Le Roux et al., 2007,” “Nguyen et al., 2018,” and “Val et al., 2012,” respectively. Additionally, the intermediate host Escherichia coli strain was named as “GEB802,” but should be “53813.”

The corrected Table 1 appears below.

TABLE 1

Table 1. Strains and plasmids used in this study.

A correction has also been made to the MATERIALS AND METHODS, Bacterial Strains, Plasmids, and Growth Conditions and Gene Disruption, paragraph one:

“To generate the sRNA disruptant, the sequence from 46 bp before the 5′ end to 2 bp after the 3′ end was deleted from the chromosome of V. alginolyticus ZJ-T. The deletion was constructed by homologous recombination as described before with some modification (Yiqin et al., 2016). Briefly, two flanking fragments of srvg23535 (Figure 1A) were amplified with two pairs of primers, srvg23535-UP-F and -R and srvg23535-DOWN-F and -R respectively, and the linearized pSW7848 was amplified with pSW7848-F and -R (Supplementary Table 1). srvg23535-UP-F and srvg23535-DOWN-R contained overlapping extensions with pSW7848-R and -F, respectively, and srvg23535-UP-R contained overlapping extensions with srvg23535-DOWN-F. The two flanking fragments were further assembled into the linearized pSW7848 by using a ClonExpress Multis One Step Cloning Kit (Vozyme, China), generating the recombinant plasmid pSW7848-Δsrvg23535 comprising the 1,084 bp upstream and 1,105 bp downstream regions of srvg23535 (Table 1), using E. coli Π3813 as an intermediate host. The recombinant plasmid was transferred by conjugation from strain GEB883 (Table 1) to V. alginolyticus ZJ-T before allelic exchange as described above. The sRNA disruptant was then confirmed by sequencing and the strain was named ZJ-T-Δsrvg23535 (Figure 1 and Table 1).”

The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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