Anti-infective efficacy of a 3D printed PLGA/HA scaffold grafted with quaternised chitosan
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1
Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, China
Contaminated or infectious bone defect remains a serious complicated challenge in current orthopedic surgery and the bone substitute with both osteoconductivity and anti-bacterial potential represents an ideal treatment strategy. In previous study, we have developed a novel quaternised chitosan (hydroxypropyltrimethyl ammonium chloride chitosan, HACC) and demonstrated that it exhibited strong antibacterial activity[1],[2]. In this study, the HACC was grafted to the 3D printed bioactive porous scaffolds, which made of polylactide-co-glycolide (PLGA) or impregnated with hydroxyapatite (HA) (weight ratio, PLGA:HA=9:1).
Three standard strains, Staphylococcus aureus (ATCC25923), Staphylococcus epidermidis (ATCC35984) and methicillin-resistant Staphylococcus aureus (ATCC43300), and one clinical isolates, methicillin-resistant S.epidermidis (MRSE287), were selected to evaluate the bacterial adhesion and biofilm formation on the materials at 4, 24 and 48h using the spread plate method, tissue culture plate (TCP) method and confocal laser scanning microscopy (CLSM) in vitro. We found that both PLGA/HA/HACC and PLGA/HACC composites could significantly inhibit bacterial adhesion and biofilm formation compared with PLGA or PLGA/HA. Meanwhile, human bone marrow derived mesenchymal stem cells were used to assess the osteoactivity of the scaffold and we found that HA impregnated scaffolds including PLGA/HA and PLGA/HA/HACC exhibited significantly better cell attachment, proliferation, spreading and osteogenic differentiation compared with the PLGA or PLGA/HACC. Furthermore, the scaffold contaminated with a bioluminescent S.aureus strain (ATCC12600, Xen29) was implanted subcutaneously in SD rats. The real-time monitoring of the IVIS Imaging System showed more evident decrease in bacterial counts in the scaffolds of PLGA/ HA /HACC and PLGA/HACC compared with the other two scaffolds without HACC grafting. Taken all together, our findings may develop a good foundation for potential clinical validation of this inovative bioactive porous scaffold for combating infectious bone defect.
NSFC-DG-RTD Joint Scheme (Project No. 51361130034); the European Union’s 7th Framework Program under grant agreement n° NMP3-SL-2013-604517
References:
[1] Peng ZX, et al. Carbohydrate Polymers 2010, 81（2）：275-2834.
[2] Tan HL, et al. Biomaterials 2012; 33:365-377.
Keywords:
Bacteria,
Infection,
3D scaffold,
Bone repair
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
New Frontier Oral
Topic:
Anti-infective biomaterials
Citation:
Yang
Y,
Yang
S,
Ao
H and
Tang
T
(2016). Anti-infective efficacy of a 3D printed PLGA/HA scaffold grafted with quaternised chitosan.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01201
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Ying Yang, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai, China, Email1
Dr. Shengbing Yang, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai, China, Email2
Dr. Haiyong Ao, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai, China, Email3