NEUROPROTECTIVE EFFECTS OF α-MANGOSTIN AGAINST SCOPOLAMINE-INDUCED COGNITIVE DEFICITS
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1
Institute of Science, Suranaree University of Technology, School of Pharmacology, Thailand
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2
Institute of Science, Suranaree University of Technology, School of Physiology, Thailand
Cholinergic neurons, particularly in the basal forebrain, are markedly depleted in Alzheimer's disease (AD) which is associ- ated with cognitive deficits. Muscarinic antagonist scopolamine (SCO)-induced cognitive deficits is commonly used as a model for AD. The extract from the fruit rind of mangosteen (Garcinia mangostana L.) was recently reported to improve spatial memory in SCO-induced amnesic rats. α-Mangostin (α-MG) is an apre- nylated xanthone derivative from the fruit rind of mangosteen. The effects of α-MG on learning and memory performance were thus investigated in SCO-induced amnesic rats. Eight groups (n=8 each) of 8-weeks-old male Wistar rats were i.p. injected with normal saline solution (1 ml/kg), donepezil (2 mg/ml/kg; positive control), α-MG (50 mg/ml/kg), or α-MG (100 mg/ml/kg) followed by i.p. injected with SCO (2 mg/ml/kg) or normal saline solution (1 ml/kg) an hour later. Thirty minutes later, the learning and memory performance were assessed using Morris water maze test. All rats received four trials per day. These procedures were repeated for 7 days. On day 7, all rats were tested in the probe trial. We found that donepezil and α-MG (50 and 100 mg/ml/kg) given to rats before SCO administration could ameliorate adverse effects of SCO by decreasing time to find platform on training session and increasing both time spent and number of entries into the target quadrant in probe trial session when compared to their control group. These findings indicated that donepezil and α-MG could improve spatial memory impairment. Pretreatment with donepezil and α-MG provided neuroprotective effects against SCO-induced memory deficits and neuronal impairment in rat brain. Future work will be required to determine whether the effects of α-MG on cholinergic system in hippocampus, cerebral cortex, and basal forebrain are related with an amelioration of the SCO-induced memory deficits.
References
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Keywords:
scopolamine,
α-Mangostin,
cognitive deficits,
Morris Water Maze (MWM),
choline acetyltransferase,
Acetylcholinesterase
Conference:
Neuroinformatics 2015, Cairns, Australia, 20 Aug - 22 Aug, 2015.
Presentation Type:
Poster, to be considered for oral presentation
Topic:
Clinical neuroscience
Citation:
Changlek
S and
Srisawat
R
(2015). NEUROPROTECTIVE EFFECTS OF α-MANGOSTIN AGAINST SCOPOLAMINE-INDUCED COGNITIVE DEFICITS.
Front. Neurosci.
Conference Abstract:
Neuroinformatics 2015.
doi: 10.3389/conf.fnins.2015.91.00061
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Received:
21 May 2015;
Published Online:
05 Aug 2015.
*
Correspondence:
Mr. Suksan Changlek, Institute of Science, Suranaree University of Technology, School of Pharmacology, Muang District, Nakhon Ratchasima, 30000, Thailand, csuksun@gmail.com