Experimental study on the effect of pine pollen on the immune system of a murine model
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1
university of 8 May 1945, Ecologie et génie de l'environnement, Algeria
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2
university of Badji Mokhtar, Biochimie, Algeria
The allergy is considered as an abnormal and specific reaction of the organism in contact with a strange substance (allergen). Actually, the allergies to pollens pose a major problem of public health seen pollinic diversity.
In order to determine the pollinic contents of the atmosphere of the commune of Oued Zenati - Guelma – Algeria, and to carry out an immunological characterization of a definite species, samplings monthly were carried out from three sites. The collection of April showed pollinic diversity with an abundance of Pine pollen. The treatment consists in sensitizing the mice with pollen by the administration (nasal way) of an extract of pine’s pollen grains. The treatment is repeated 5 times at the order of once / 3 days during two weeks. After this treatment, various analyses (nasal lavage, bronco-alveolar lavage, white blood cells and histological study) were realized. The smears of nasal and bronco-alveolar liquids from different mice (treated and control) were made and stained with May-Grünwald-Giemsa.
This experiment has allowed us to identify the changes of some immunological parameters such as an increase in the total number of the nasal and bronco-alveolar liquid cells, a reduction in the rate of the white cells, lymphocytes, monocytes and polynuclears, as well as an increase in the thrombocytes in the animals treated with the pine pollen
The organism deteriorations in the organization have been demonstrated by the increase in lungs weight because of their inflammatory state confirmed by a histological study proving the presence of the inflammatory infiltrates in the sensitized mice.
In our work, we have tried to contribute to the study pollen allergies. We investigated the effects of pine pollen on the respiratory immune system (nasal and bronco-alveolar liquid) and the WBC. These results illustrate the effect of pollen on the immune system depending on the type of pollen and its allergenic power which still interesting to be exploited.
References
Durham O.C. (1946): “The volumetric incidence of atmospheric allergens. IV. A proposed standard method of gravity sampling, counting and volumetric interpolation ofresults”. J. Allergy, 17(2): 79-86.
Xiuzhen S., Yali L., Lei Z., Yun L. & Xia Y. (2001): Humulus pollen allergic pulmonary inflammation model mice sensitized. Chinese Journal of Microbiology and Immunology, S(2): 1-7.
Urbain B., Mast J., Goddeeris B., Ansay M. & Gustin P. (1997): Influence d’une exposition aux poussières sur la composition cellulaire des liquides de lavage nasal et broncho-alvéolaire chez le porc. Journées Rech. Porcine en France, 29 : 17-22.
Keywords:
allergy,
bronco-alveolar lavage,
Immune reactions,
Nasal Lavage,
pollens
Conference:
The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014.
Presentation Type:
Poster Presentation
Topic:
Allergology
Citation:
BOUGUENOUN
I,
Bensakhri
Z,
MERZOUG
S,
BOUGUENOUN
W and
BENDJEDDOU
D
(2014). Experimental study on the effect of pine pollen on the immune system of a murine model.
Front. Immunol.
Conference Abstract:
The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014).
doi: 10.3389/conf.fimmu.2014.04.00005
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Received:
09 Sep 2014;
Published Online:
01 Dec 2014.
*
Correspondence:
Miss. Imane BOUGUENOUN, university of 8 May 1945, Ecologie et génie de l'environnement, Guelma, 24000, Algeria, imeneb87@yahoo.com