Effect of SAA1, SAA2 and SAA4 knockdown on proliferation and invasion of glioblastomas multiformes cells
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1
Universidade de São Paulo, Brazil
Serum amyloid A (SAA) is an immunomodulatory protein produced in acute and chronic inflammatory process, such as cancer. Our research group showed that glioblastomas multiformes - A172 and T98G cells - produce SAA and have proliferation, migration and invasion affected by SAA treatment (Knebel, et al. Mediators of Inflammation, 2013, in press). To understand the significance of SAA produced by the tumor itself or by an inflammatory process, SAA1, SAA2 and SAA4 genes knockdown were prepared by lentiviral system using five different plasmids for each SAA gene. 3[H] thymidine incorporation and Boyden chamber assay were used for proliferation and invasion measurements, respectively. Among the five plasmids of each gene, we were able to selected at least one that present a gene inhibition greater than 88%. SAA1 and SAA2 knockdown inhibited proliferation and invasion of glioblastomas multiformes cells when compared to the wild type. However the SAA4 knockdown only inhibited the A172 proliferation. Although preliminary, our data may suggest that SAA1 and SAA2 contribute more than SAA4 to tumor invasiveness. Addition of recombinant SAA (Peprotech) revert the effects of silencing in most but not all cases. At this point it is crucial to identify the roles of each SAA genes and the genetic or cellular uniqueness that outline SAA responsiveness to identify the potentiality of SAA as a therapeutic target in cancer.
Acknowledgements
Grant support: Sao Paulo Research Foundation (FAPESP, Sao Paulo); National Counsel of Technological and Scientific Development (CNPq, Brasília); and Coordination of Improvement of Higher Education Personnel (CAPES, Brasília).
References
Knebel, F.H., Albuquerque, R.C., Massaro, R.R., Maria-Engler, S.S., Campa, A. (2013). Dual Effect of Serum Amyloid A on the Invasiveness of Glioma Cells. Mediators of Inflammation.
Keywords:
Serum amyloid A knockdown,
Inflammation,
proliferation,
invasion,
glioblastomas multiformes
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune receptors and signaling
Citation:
KNEBEL
FH,
Bellé
LP,
Haga
R,
Kaihami
G and
Campa
A
(2013). Effect of SAA1, SAA2 and SAA4 knockdown on proliferation and invasion of glioblastomas multiformes cells.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00949
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Received:
26 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Ms. FRANCIELE H KNEBEL, Universidade de São Paulo, São Paulo, Brazil, fhknebel@usp.br