Haplotype analysis of interleukin-8 gene polymorphisms in chronic and aggressive periodontitis
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1
Faculty of Medicine, Masaryk University, Brno, Department of Pathophysiology, Czechia
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2
Faculty of Medicine, Masaryk University, Brno, Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Czechia
Objective: Periodontitis is an inflammatory disease characterized by connective tissue loss and alveolar bone destruction. Interleukin-8 (IL-8) variants are important in the regulation of the inflammatory response. The aim of this study was to analyze four polymorphisms in the IL8 gene and their haplotypes in relation to chronic (CP) and aggressive periodontitis (AgP).
Design: A total of 492 unrelated subjects were included in this case-control study. Genomic DNA of 278 patients with CP, 58 patients with AgP, and 156 healthy/non-periodontitis controls were genotyped, using the 5′ nuclease TaqMan® assay for allelic discrimination, for IL8 (rs4073, rs2227307, rs2227306, rs2227532) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray based on a periodontal pathogen detection kit in a subgroup of subjects (N=114).
Results: Allele and genotype frequencies of all investigated IL8 polymorphisms were not significantly different between the subjects with CP and/or AgP and controls (p > 0.05). Nevertheless, the complex analysis revealed differences in IL8 haplotype frequencies. Specifically, the haplotypes A(-251)/T(+396)/T(+781) and T(-251)/G(+396)/C(+781) were significantly less frequent in patients with CP (2.1% versus 5.5%, resp. 4.9%, p < 0.05) and haplotype T(-251)/G(+396)/C(+781) was significantly less frequent in patients with AgP (0.9% versus 4.9%, p < 0.05) than in controls.
Conclusions: Although none of the investigated SNPs in the IL8 gene was individually associated with periodontitis, some haplotypes can be protective against CP and AgP in the Czech population.
Acknowledgements
This study was supported by the project IGA NT11405-6.
Keywords:
Interleukin-8,
IL8 gene polymorphism,
Bacterial colonization,
Chronic Periodontitis,
Aggressive Periodontitis
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Borilova Linhartova
P,
Vokurka
J,
Poskerová
H,
Fassmann
A and
Izakovičová Hollá
L
(2013). Haplotype analysis of interleukin-8 gene polymorphisms in chronic and aggressive periodontitis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00919
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Received:
25 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Lydie Izakovičová Hollá, Faculty of Medicine, Masaryk University, Brno, Department of Pathophysiology, Brno, Czechia, holla@med.muni.cz