Phenotypic and functional heterogeneity of circulating CD4+ and CD8+ T cell subsets and their age related changes
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1
Universidad de Antioquia, Colombia
The phenotypic heterogeneity of human CD4+ and CD8+ T cell subsets has been widely reported. Many different surface markers are currently used to identify T cell subsets, resulting in a rather confuse panorama. We analyzed the phenotype of
circulating CD4+ and CD8+ T cell subsets in healthy individuals, based on the expression of CD45RO and CD27. Thus, four
CD4+ and CD8+ T cell subsets were initially identified: CD45RO+CD27+ (TCM), CD45RO+CD27- (TEM), CD45RO-CD27+ (Tnaïve/early) and CD45RO-CD27- (TEF). The percentage of circulating CD4+ and CD8+ T cell subsets showed a higher frequency of circulating CD4+TCM compared to CD8+ cells, and of CD8+TEF cells compared to CD4+ cells. There was a significant negative correlation between CD4+ Tnaïve/early and CD4+TEM, and between CD8+ Tnaïve/early cells and CD8+TCM. Further analysis confirmed a high heterogeneity regarding the expression CD127, CD62L, CD45RA, CD28 and CD57; the later mainly expressed by CD27- T cells. The functional evaluation of T cell subsets showed that memory T cells (TCM and TEM) were the main responders to the stimulation with anti-CD3 plus anti-CD28 and PPD. Finally, significant age-related changes in the frequency of T cell subsets were associated with a reduction in the frequency of Tnaïve/early and an increase in the frequency of memory T cells, mainly TEM. Our results corroborate the heterogeneity of CD4+ and CD8+ T cell subsets and their age-related changes; however, their biological meaning requires careful analysis and interpretation.
Acknowledgements
This work was supported by COLCIENCIAS (Bogotá, Colombia), grant 1115408204088.
Keywords:
T cells memory,
Aging,
C,
CD4+,
CD8+
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Marín
ND,
Rojas
M,
París
SC and
Garcia
LF
(2013). Phenotypic and functional heterogeneity of circulating CD4+ and CD8+ T cell subsets and their age related changes.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00753
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Received:
13 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Luis F Garcia, Universidad de Antioquia, Medellin, Colombia, lfgarcia@une.net.co