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Basophils control T cell Responses and limit Disease Activity in Experimental Murine Colitis

Manuel Rodriguez Gomez1*, Yvonne Talke1, Claudia Hofmann2, Isabel Ketelsen1, Fabian Hermann1, Barbara Reich1, Kathrin Schmidbauer1, Nicole Goebel1, Nadja Dunger1, Hilke Bruehl2, Kerstin Renner1, Shahzad-Nawaz Syed1 and Matthias Mack1
  • 1 University Clinic Regensburg, Internal Medicine II - Nephrology, Germany
  • 2 University Clinic Regensburg, Internal Medicine I, Germany

Basophils have been recognized as important inducers of Th-2 responses. Using the colitis model of adoptive transfer of CD4+ CD62L+ T cells into lymphopenic hosts we have analyzed how basophils regulate T cell responses and modulate disease activity. Transferred T cells rapidly proliferate, produce large amounts of IL-3 and expand the number of basophils in an IL-3 dependent manner. Depletion of basophils with two different antibodies substantially upregulated Th-1 cytokines in transferred T cells at day 8. Increased Th-1 cytokine expression persisted until the end of the experiment when basophil depleted mice showed exacerbation of colitis with more severe loss of weight, histological damage, colonic leukocyte infiltration and expression of proinflammatory cytokines. In vitro we show that basophil derived IL-4 and IL-6 downregulates expression of IFN-γ, IL-2 and TNF in T cells. These data show a beneficial role of basophils in a T cell driven model of autoimmunity.

Acknowledgements

We thank Glenn Dranoff for the permission to use the IL-3 deficient mice. We thank Maria Hutterer for help in histology, Rüdiger Eder and Jasmin Stahl for support in FACS-sorting and Klaus Stark for help with real time PCR. This work was supported by grants from the Deutsche Forschungsgemeinschaft (M.M.)

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Keywords: basophil, Colitis, T cell, Autoimmunity, Interleukin-3, Interleukin-4, Interleukin-6, Interferon-gamma, Tumor Necrosis Factor-alpha

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Adaptive Immunity

Citation: Rodriguez Gomez M, Talke Y, Hofmann C, Ketelsen I, Hermann F, Reich B, Schmidbauer K, Goebel N, Dunger N, Bruehl H, Renner K, Syed S and Mack M (2013). Basophils control T cell Responses and limit Disease Activity in Experimental Murine Colitis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00641

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Received: 11 Jun 2013; Published Online: 22 Aug 2013.

* Correspondence: Dr. Manuel Rodriguez Gomez, University Clinic Regensburg, Internal Medicine II - Nephrology, Regensburg, 93053, Germany, manuel.rodriguez-gomez@klinik.uni-regensburg.de