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Biochemical and cellular correlates of embelin against bacterial endotoxin induced airway inflammation

Madhusudan N. Saraf1*, Rahul Shirole1, Lohit B. Badgujar1 and Ashish Kshatriya1
  • 1 Bombay College of Pharmacy, Dept. Pharmacology, India

To investigate mechanism of anti-inflammatory activity of embelin against bacterial endotoxin induced acute lung inflammation.
Embelin was isolated from dried seeds of Embelia ribes. Female Sprague Dawley rats (180-230g) were divided in six groups. Saline (5ml/kg/day,p.o.) Embeline (5,10&20mg/kg/day,p.o.), Roflumilast (1mg/kg/day,p.o.) were administered for four days. Animals were anesthetised with ketamine-xylazine (80:20 mg/kg,i.p.) and administered with sterile solution of LPS (Lipopolysaccharide) intratracheally. After four hours bronchoalveolar lavage was done with icecold phosphate buffer. Bronchoalveolar lavage fluid (BALF) and lung samples were investigated for total cell count, differential cell count and myeloperoxidase, nitrate/nitrite, total Protein, albumin, histopathology respectively.
Data was analysed by GraphPad Prism 5.01 and results were expressed as means ± SEM. P value of <0.05 was considered to be significant.
Saline treated animals showed significant increase in total and differential cell count (p<0.05). Embelin (5,10&20mg/kg) & Roflumilast (1mg/kg) treated animals showed significant decrease in total and differential cell count compared to saline treated animals (p<0.05).
Saline treated animals showed significant increase in myeloperoxidase, nitrosative stress, albumin and total protein in lung samples but embelin (5, 10 & 20mg/kg) and roflumilast (1mg/kg) showed reduction compared to saline treated animals (p<0.05). Mild diffused lesions involving focal interalveolar septal, intraluminal infiltration of neutrophils were observed in embelin (5&10 mg/kg) pretreated while no abnormality was detected in embelin (20 mg/kg) rofelumilast (1mg/kg) pretreated rats.
Embelin showed anti inflammatory and protective effect against LPS induced airway inflammation by reducing nitrosative stress and inhibiting mononucleated cellular infiltration. Further studies on molecular level are in progress.

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Keywords: Bacterial endotoxin, airway inflammation, Embelin, Embelia ribes, LPS

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Translational immunology and immune intervention

Citation: Saraf MN, Shirole R, Badgujar LB and Kshatriya A (2013). Biochemical and cellular correlates of embelin against bacterial endotoxin induced airway inflammation. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00571

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Received: 02 May 2013; Published Online: 22 Aug 2013.

* Correspondence: Dr. Madhusudan N Saraf, Bombay College of Pharmacy, Dept. Pharmacology, Mumbai, Maharashtra, 400098, India, madhusudan.saraf@gmail.com