HMGB1 in an experimental model of acute lung injury.
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1
Botucatu Medical School - São Paulo State University - UNESP, Brazil
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2
Bioscience Institute of Botucatu - UNESP, Microbiology and Immunology, Brazil
Despite recent therapeutic advances in acute respiratory distress syndrome (ARDS), its physiological and immunological aspects have not been completely understood. Recently, HMGB1, a nonhistone, chromatin-associated protein and a cytokine produced by macrophages, has been associated with sustained inflammatory response after injury or infection of tissue and lung injury. The aim of the study was to measure HMGB1 levels in rabbits with acute lung injury (ALI) induced by tracheal infusion of warm saline.
Methods: Thirty rabbits were instrumented and randomly assigned into two groups: 1) animals with no ALI (Control Group-CG; n = 15); 2) animals with severe ALI (SG Group; n = 15). The groups were ventilated during 4 hours with protective conventional mechanical ventilation (CMV). After ventilation, HMGB1 levels from bronchoalveolar lavage fluid (BAL) and plasma were measured as well as the percentage of white cell from BAL. Left lung was collected for histopathology analysis.
Results:Lung injury decreased pulmonary compliance (SG before: 1.86±0.576 > SG after: 0.67± 0.24) and oxygenation (PaO2 - SG before: 427.92 ± 89.90 > SG after: 68.18 ± 19.08). Percentage of neutrophil (p=0.001) and lung injury score were higher in SG than CG. Higher levels of HMGB1 in BAL were found in SG as compared to CG group [SG: 62 (20.30-79.41) > CG: 15.60 (13.86-25.81); p<0.035]. No statistically significant difference was found in HMGB1 plasmatic levels when groups were compared.
Conclusions:HMGB1 is released earlier in alveolar space in acute lung lesion induced by warm saline and can contribute to the pathogenesis of ALI.
Fapesp grants:2011/15144-2
Acknowledgements
This study was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo);grants 2011Q15144-2. We would like to thank Clinical and Experimental Research Center of Pediatrics Department
Keywords:
HMGB1,
lung lesion,
rabbit,
Lung Diseases,
Cytokines
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Kurokawa
CS,
Fioretto
JR,
Araujo
JP,
Pires
RB,
Moraes
MA,
Bonatto
RC and
Carpi
MF
(2013). HMGB1 in an experimental model of acute lung injury..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00525
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Received:
31 May 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Cilmery S Kurokawa, Botucatu Medical School - São Paulo State University - UNESP, Botucatu, Sao Paulo, 18618970, Brazil, cskurokawa@gmail.com