Plasmacytoid Dendritic Cells control tumor progression in LPS-stimulated lung tumor-bearing mice.
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1
University of Salerno, Department of Pharmacy (DIFARMA), Italy
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2
Cedars Sinai Medical Center, United States
The anti-tumour activity of LPS was firstly described by William Coley. However its role in lung cancer is still unclear. The aim of our study was to elucidate the effect of LPS (0.1-10µg/mouse) in a mouse model of B16-F10-induced metastatic lung cancer. Lung tumour growth increased at 3 and 7 days after the administration of low dose of LPS (0.1µg/mouse). This was associated with a greater influx of plasmacytoid dendritic cells (pDCs), regulatory T-cells (Treg), myeloid-derived suppressor cells (MDSC) and CD8+ Treg cells. In contrast, the high dose of LPS (10µg/mouse) reduced lung tumour burden associated with higher influx of pDCs, Th1- and Th-17 polarization. Depletion of pDCs using a specific antibody (m927) reversed the immune-suppressive environment associated to the administration of the low dose of LPS and resulted in a decreased lung tumour burden. The opposite was observed with high dose of LPS. The dichotomy in LPS activity was due to the phenotype of pDCs, which were immune-suppressive after the low dose, and Th1- and Tc-polarizing cells after the high dose administration. The adoptive transfer of T cells into nude mice demonstrated that CD8+ T cells were responsible for pDC recruitment after the administration of the low dose, whereas CD4+ T cells were dispensable for pDC influx after the high dose of LPS.
In conclusion, our data demonstrate the dual activity of LPS according to pDC phenotype into the lung of tumour-bearing mice.
Acknowledgements
This work was supported by FARB from the university of Salerno and by Campania Research in Experimental Medicine (CREME) POR Campania FSE 2007-2013. RS was supported by a fellowship from the University of Salerno, Italy.
Keywords:
LPS,
lung cancer,
plasmacytoid dendritic cells,
innate immunity,
Immunoediting
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Sorrentino
R,
Rega
A,
Terlizzi
M,
Forte
G,
Crother
T,
Arditi
M and
Pinto
A
(2013). Plasmacytoid Dendritic Cells control tumor progression in LPS-stimulated lung tumor-bearing mice..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00357
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Received:
20 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Rosalinda Sorrentino, University of Salerno, Department of Pharmacy (DIFARMA), Fisciano-Salerno, Italy, rsorrentino@unisa.it