Ursolic acid effect on proinflammatory cytokines in response to diet-induced obesity in rats
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1
UNIVERSIDAD DE GUADALAJARA, Instituto de Enfermedades Crónico Degenerativas, Mexico
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2
Universidad de Guadalajara, Instituto de Biología Molecular y Terapia Génica, Mexico
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3
Universidad de Guadalajara, Grupo de Inmunogenética Funcional, Mexico
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4
Universidad de Guadalajara, Laboratorio de Ciencias de los Alimentos, Mexico
BACKGROUND. Obesity represents a major metabolic alteration of worlwide impact. Inflammation constitutes a molecular feature in obesity mediated by the expression of TNF-α, IL-1 and MCP1, among other proinflammatory cytokines, which contributes to the development of associated morbidities. Antiinflammatory activity of Ursolic acid (UA), a pentacyclic triterpenoid, has been evidenced both in vivo and in vitro models. OBJECTIVE. The aim of this work was to evaluate the antiinflammatory effect of UA on diet-induced obese rats. MATERIAL AND METHODS. Antiinflammatory preventive effect of AU was assayed by intragastrically administering UA (50 mg/kg/day) together with a high-fat diet (HFD) to Wistar rats weighing 150-180 g during 3, 6, or 9 weeks. Antiinflammatory reversal effect of AU was evaluated by administering AU (50 mg/kg/day) during 3 weeks to previously HFD fed animals during 6 weeks. Control group received only HFD. ELISA and QPCR to quantify proteins and cytokines expression respectively, were performed. RESULTS. Comparisons were always assessed between experimental and control group. Adipose tissue relative expression of TNF-α was statistically lower (p<0.05) after 9 weeks of preventive treatment. Significantly (p<0.05) lower expression of IL-1 and MCP-1 was appreciated after 6 and 9 weeks of preventive treatment and also after reversal treatment. Circulating levels of TNF-α and IL-1 were not detectable. CONCLUSIONS. AU is capable to counteract obesity-induced inflammatory cytokine expression in animals fed HFD.
Keywords:
Macrophages,
antiinflammatory effect,
high fat diet,
obesity model,
cytokine expression.
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
González-Garibay
AS,
López-Vazquez
A,
Garcia-Bañuelos
J,
Sandoval-Rodríguez
AS,
Muñoz-Valle
JF,
Bueno-Topete
MR,
Del Toro-Arreola
S,
Del-Angel-Meza
AR,
Armendáriz-Borunda
JS and
Bastidas-Ramírez
BE
(2013). Ursolic acid effect on proinflammatory cytokines in response to diet-induced obesity in rats.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00261
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Received:
14 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Blanca E Bastidas-Ramírez, UNIVERSIDAD DE GUADALAJARA, Instituto de Enfermedades Crónico Degenerativas, GUADALAJARA, Mexico, bastidas@cencar.udg.mx