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Effects of sinapic acid on oxidative DNA damage in V79 cell line

Hasan Hameed1, Sevtap Aydin2*, Arif Ahmet Başaran3 and Nursen Basaran2
  • 1 Basra University, Department of Pharmacology, Faculty of Pharmacy, Iraq
  • 2 Hacettepe University, Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Türkiye
  • 3 Hacettepe University, Department of Pharmacognosy, Faculty of Pharmacy, Türkiye

Phenolic compounds, due to their antioxidant properties, play an important role in the prevention of various degenerative disorders or diseases related to oxidative damage. Sinapic acid (SA), a phenolic compound, is widely distributed in the plant kingdom and commonly consumed in human diets. SA has been described as a chain-breaking antioxidant that probably acts as a radical scavenger. SA was reported to exhibit a protection against H2O2-mediated cytotoxicity in a dose-dependent manner. SA is believed to be therapeutically beneficial and non-toxic. However the data about the genotoxicity of SA are limited. In this study, the genotoxic/antigenotoxic activities of SA were evaluated in V79 cells by alkaline single cell gel electrophoresis. No significant increase in DNA strand breakage expressed as DNA tail intensity was observed below 1000 µM; however, at the concentrations of 1000-5000 µM SA alone caused an increase in DNA damage in a dose-dependent manner. At the concentrations of 50-2000 µM, SA seemed to significantly decrease H2O2-induced DNA damage. However, at the highest concentration of 5000 µM, SA did not decrease H2O2-induced DNA damage in V79 cells. In conclusion, at low concentrations SA might protect against various oxidative stress related-diseases by reducing oxidative DNA damage.

Acknowledgements

This study was funded in part by a grant from Hacettepe University Research Fund (contract grant number: 014 D11 301 002).

References

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Keywords: Sinapic acid, Chinese hamster lung fibroblast cell line, DNA Damage, Oxidative Stress, Alkaline single cell gel electrophoresis

Conference: ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015.

Presentation Type: Poster Presentation

Topic: Genotoxicity testing of chemicals and nanomaterials

Citation: Hameed H, Aydin S, Başaran A and Basaran N (2015). Effects of sinapic acid on oxidative DNA damage in V79 cell line. Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop. doi: 10.3389/conf.fgene.2015.01.00041

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Received: 14 Apr 2015; Published Online: 23 Jun 2015.

* Correspondence: Dr. Sevtap Aydin, Hacettepe University, Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ankara, 06100, Türkiye, sevtapaydin@hotmail.com