Hierarchically aligned fibrillar fibrin hydrogel designed for spinal cord injury repair
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1
Tsinghua University, School of Materials Science and Engineering, China
Biomaterial-based strategies for nerve regeneration have attracted extensive attentions in recent years. In this study, a hierarchical aligned fibrillar fibrin hydrogel was prepared through electrospinning technique and the concurrent molecular self-assembly process at the moment of collection. The aligned fibrin hydrogel that simulated the elasticity and oriented structures of nerve tissue simultaneously provided biomimetically biophysical cues to direct in vivo or in vitro cells thus constructing nerve-like microtissues rapidly. In vitro studies showed that human umbilical cord mesenchymal stem cells could “feel” the cues afforded by aligned fibrin gels and extend along the long axis of the fibers soon after cell attachment and undergo strong neural differentiation after two weeks cell culture in regular growth medium without any soluble growth factors. More interestingly, the rat dorsal root ganglion neurons also could follow the fiber structure in a highly oriented longitudinal manner with three-dimensionally neurite elongation to total 3.92 mm just in 3 days basic culture. In rat T9 dorsal hemisection spinal cord injury. the host neural cells with β-tubulin III-positive migrated into the aligned fibrin hydrogels very quickly. And the axon reconstruction and locomotor performance was improved starting from 2 weeks after the surgery in the aligned fibrin hydrogel group. Our study suggested that the hierarchically aligned fibrillar fibrin hydrogels will not only have great promising for directing stem cells towards nerve lineage, but also may have great potential applications in spinal cord regeneration.
Tsinghua University Initiative Scientific Research Program (20121087982, 20131089199); 973 Program (No. 2011CB606205)
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Keywords:
Hydrogel,
Regenerative Medicine,
Biomimetic,
matrix-cell interaction
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomimetic materials
Citation:
Wang
X and
Yao
S
(2016). Hierarchically aligned fibrillar fibrin hydrogel designed for spinal cord injury repair.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01340
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Xiumei Wang, Tsinghua University, School of Materials Science and Engineering, Beijing, China, Email1
Dr. Shenglian Yao, Tsinghua University, School of Materials Science and Engineering, Beijing, China, csuysl@126.com