Design of viral-mimetic surfaces to recognize tumor glycolipids using hemagglutinating virus of japan envelope (HVJ-E)
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1
National Institute for Materials Science (NIMS), Biomaterials Unit, Japan
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2
Osaka University Hospital, Medical Center for Translational Research, Japan
Our study reports a versatile immobilization method of Hemagglutinating Virus of Japan Envelope (HVJ-E) for the generation of viral-mimetic surfaces for metastatic prostate cancer cells isolation.
HVJ-E has recently attracted much attention as a new type of therapeutic materials because metastatic prostate cancer cells such as PC-3 cells possess the HVJ-E receptors, GD1a. The HVJ-E was successfully immobilized on precursor films composed of poly-L-lysine and alginic acid via layer-by-layer assembly without changing the biological activity. The monolayer adsorption of HVJ-E particles was confirmed by quartz crystal microbalance, fluorescent and atomic force microscopy analyses.
By developing the HVJ-E coating with an affinity based cell trap within a glass capillary tube, we are able to gently isolate PC-3 from LN-Cap cells that represent adenocarcinoma without compromising cell viability. We achieved approximately 100 % cell separation efficiency only by 60 seconds of flowing. We believe that the proposed technique offers significant promise for the creation of a metastatic cancer cells trap on a broad range of materials.
References:
[1] T. Okada, K. Uto, M. Sasai, C. M. Lee, M. Ebara and T. Aoyagi, Langmuir, 2013, 7384–7392.
Keywords:
virus,
Polymeric material,
Cell interaction,
bioactive interface
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Layer-by-layer deposition techniques
Citation:
Okada
T,
Uto
K,
Aoyagi
T,
Lee
C and
Ebara
M
(2016). Design of viral-mimetic surfaces to recognize tumor glycolipids using hemagglutinating virus of japan envelope (HVJ-E).
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01118
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.