Impact of glacial refugia on present-day diversity of the Black Sea trout (Salmo labrax Pallas, 1814) in Croatia
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1
Division of Zoology, Faculty of Science, University of Zagreb, Croatia
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2
Faculty of Science, University of Zagreb, Department of Zoology, Croatia
Gene structure and diversity emerged from evolutionary history of species represent a valuable and fragile gene reservoir crucial for dealing with environmental changes, as well as handy tool for discovering one species evolutionary history. Complete sequencing of the mitochondrial cytochrome b gene (cyt b) of Black Sea trout collected among Adriatic and Danube watershed in Croatia was used to describe phylogenetic and intraspecies diversity, population structure and viability, and consequently to describe colonization of this species in Croatia. In this paper we report on analysis based on cyt b gene of 414 different individuals collected on 92 different localities in order to define geographic distribution of this species in Croatia along with its phylogenetic status and populations structure. Phylogenetic reconstruction was performed using MP (maximum parsimony) and ML (maximum likelihood) phylogenetic methods, along with MJ (median-joining) algorithm which resulted in phylogenetic network. To adequately describe status and stability of populations, effective population sizes and migration rates were estimated using MIGRATE 3.2.1, intraspecific genetic diversity was approximated with DnaSP 5.10 Software as well as viability of populations, simulated with Vortex 10 software. Finally, evolutionary history of Black Sea trout was analyzed using BEAST software. Sequencing of cytochrome b gene revealed 43 different haplotypes and an interesting evolutionary pattern which was deeply affected by alternation between glacial and interglacial periods. Also, intraspecific genetic diversity indicated high variety of this species, making this area a “gene pool hot spot”, with numerous localities containing pure populations of Salmo labrax, even though some of them did not stay immune to either natural or anthropological pressures. All in all, considering mtDNA and population analyses, it seems like this particular area played a major role in present species distribution and evolution, most probably owing it to its specific geology.
Keywords:
Black Sea trout,
cytochrome b,
evolutionary history,
Glacial – Interglacial,
hot spot,
Salmo labrax,
Population viability,
population structure,
genetic diversity
Conference:
XVI European Congress of Ichthyology, Lausanne, Switzerland, 2 Sep - 6 Sep, 2019.
Presentation Type:
Oral
Topic:
GENETICS, GENOMICS AND PHYSIOLOGY
Citation:
Ivić
L,
Buj
I and
Raguz
L
(2019). Impact of glacial refugia on present-day diversity of the Black Sea trout (Salmo labrax Pallas, 1814) in Croatia.
Front. Mar. Sci.
Conference Abstract:
XVI European Congress of Ichthyology.
doi: 10.3389/conf.fmars.2019.07.00052
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Received:
07 Jun 2019;
Published Online:
14 Aug 2019.
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Correspondence:
PhD. Lucija Ivić, Division of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia, lucija.ivic@biol.pmf.hr