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THYMOQUINONE AMELIORATES NICOTINE-INDUCED SPERM DAMAGE IN RATS

Farah Dayana Rosli1*, Noor Hashida Hashim2, Yusmin Mohd Yusuf3, Khairul Osman4, Siti Fatimah Ibrahim5 and Nurul Kabir6
  • 1 University of Malaya, Institute of Graduate Studies, Malaysia
  • 2 University of Malaya, Biology Division, Center for Foundation Studies in Science, Malaysia
  • 3 University of Malaya, Centre for Research in Biotechnology for Agriculture (CEBAR), Malaysia
  • 4 National University of Malaysia, Faculty of Allied Health Sciences, Malaysia
  • 5 National University of Malaysia, Faculty of Medicine, Malaysia
  • 6 University of Malaya, Institute of Biological Sciences, Malaysia

Background Thymoquinone (TQ), the main constituent of the volatile oil derived from Nigella sativa has demonstrated therapeutic potentials against various diseases while nicotine is an active component in cigarette known to be detrimental towards sperm quality. This study was conducted to assess the effects of TQ in ameliorating the injurious state in sperm of nicotine treated rats. Methods Adult male Sprague-Dawley rats were divided into four equal groups: control group received normal saline orally for 60 days; nicotine group was subcutaneously injected with 5 mg/kg/day nicotine for 30 days and then given normal saline for next 30 days; TQ group was given normal saline for 30 days followed by TQ 5 mg/kg/day for 30 days; nicotine-TQ group, received 5 mg/kg nicotine for 30 days, followed by TQ 5mg/kg for 30 days. The sperm count, motility and mitochondrial function were evaluated. Results Sperm count was significantly lower (p<0.05) in the nicotine group (26.72 ± 1.64) in comparison to the control (31.75 ± 0.80). Whereas the number of sperm was significantly higher in the nicotine-TQ group (30.97 ± 0.88) compared to the nicotine group. Motility evaluation demonstrated similar pattern with number of motile sperm was higher in the nicotine-TQ group (85.02 ± 2.24 %) compared to the nicotine group (66.24 ± 1.01 %). In mitochondrial function assessment, nicotine-TQ group (79.08 ± 0.74 %) showed higher (p<0.05) MitoTracker positive sperm percentage compared to nicotine group. Conclusion Thus, the present study suggests the potential of TQ in ameliorating nicotine-induced sperm damage.

Acknowledgements

Gratefully acknowledging UMRG, project number RG378/15AFR for the funding of this project. Immense gratitude to Faculty of Medicine , UKM for the usage of facilities.

References

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Keywords: Thymoquinone, Nicotine, Sperm Count, motility, Mitochondria

Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.

Presentation Type: Poster Presentation

Topic: Miscellaneous

Citation: Rosli F, Hashim N, Mohd Yusuf Y, Osman K, Ibrahim S and Kabir N (2019). THYMOQUINONE AMELIORATES NICOTINE-INDUCED SPERM DAMAGE IN RATS. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2019.63.00001

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Received: 28 Nov 2018; Published Online: 17 Jan 2019.

* Correspondence: Miss. Farah Dayana Rosli, University of Malaya, Institute of Graduate Studies, Kuala Lumpur, Malaysia, farahdayanarosli@yahoo.com