Antibacterial activity of silver nanoparticles Clinacanthus nutans (Agnp-CN) against Streptococcus mutans
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1
Department of Basic Sciences & Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Malaysia
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2
Cluster of Integrative Medicine, Advanced Medical and Dental Institute, University of Science, Malaysia, Malaysia
Background
Clinacanthus nutans was found to possess anti-venom, anti-inflammatory, analgesic, anti- diabetic, anti-rheumatism, antiviral and antioxidant properties. Silver nanoparticles are nanoparticles between 1nm to 100nm in size and play significant role in medicinal fields. Silver nanoparticles exhibit distinctive properties, such as good conductivity, chemical stability, catalytic and antibacterial activity. Streptococcus mutans is commonly found in human oral cavity and the main contributor to tooth decay. There is no study on antibacterial effect of silver nanoparticles Clinacanthus nutans (AgNp-CN) against Streptococcus mutans reported to date. Therefore, objective of this study is to investigate antibacterial properties of silver nanoparticles Clinacanthus nutans against Streptococcus mutans.
Methods
Streptococcus mutans was subcultured in brain heart infusion (BHI) broth and agar. AgNp-CN with different concentrations was tested against Streptococcus mutans via disc diffusion assay, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Amoxicillin was used as positive control while DMSO and blank silver nanoparticles were used as negative control.
Results
Disc diffusion assay shows AgNp-CN inhibit Streptococcus mutans growth. AgNp-CN shows the greatest inhibition properties (15.3±1.53mm) in comparison to Clinacanthus nutans leaves extract (6.0±0.00mm) and blank silver nanoparticles (6.0±0.00mm). MIC value for AgNp-CN is 2.5mg/mL while amoxicillin is 25mg/mL. Whereas MBC values for AgNp-CN is 2.5mg/mL. Results are concentration dependent, with higher concentration shows better inhibition property.
Conclusion
It can be concluded AgNp-CN possesses bactericidal properties against Streptococcus mutans.
Keywords:
Tooth decay,
Antibacterial,
silver nanoparticles,
Streptococcus mutans,
Clinacanthus nutans
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Miscellaneous
Citation:
Abd Ghafar
S,
Muhd Fudzi
N,
Wan Sulaiman
W,
Mohamad Hanafiah
R and
Lim
V
(2019). Antibacterial activity of silver nanoparticles Clinacanthus nutans (Agnp-CN) against Streptococcus mutans.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00062
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Received:
12 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Siti Aisyah Abd Ghafar, Department of Basic Sciences & Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Nilai, Malaysia, aisyahghafar@usim.edu.my