Artificial dense granules, a potential, biomimetic procoagulant agent composed of inorganic polyphosphate, initiates autoactivation of factor XII
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1
University of Illinois at Chicago, Chemical Engineering, United States
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2
University of Illinois at Chicago, Physics, United States
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3
University of Illinois at Urbana-Champaign, Biochemistry, United States
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4
University of Illinois at Chicago, Biopharmaceutical Sciences, United States
Platelet-sized, inorganic polyphosphate (polyP) was nanoprecipitated in aqueous media and encapsulated in sterically-stabilized liposomes, creating a liposomal nanoformulation with promising procoagulant properties, reminiscent of human platelet dense granules’ hemostatic effects. Dynamic light scattering (DLS) measurements reveal that the Artificial Dense Granules (ADGs) are 200-nm monodisperse particles, and are stable in suspension for several hours. High-resolution imaging accomplished by placing the ADGs in a biocompatible graphene sandwich demonstrates that the nanoformulation possesses a defined core-shell nano-architecture, consisting of a 150-nm granular core with a homogeneous distribution of Ca, P, and O and a 25-nm shell containing only C, P, and O. Triggered autoactivation of Factor XII (FXII) zymogen was realized in vitro by detergent solubilization or phospholipase-induced hydrolysis of the ADG liposomal envelope on a time-scale sufficient to mitigate adverse outcomes arising from hemorrhagic bleeding events.
Ms. Linda Juarez; Dr. Qiao Qiao
Keywords:
nanoparticle,
biomedical application,
targeting delivery,
biomacromolecule
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Tissue targeting nanoparticles
Citation:
Donovan
AJ,
Kalkowski
J,
Szymusiak
M,
Wang
C,
Smith
SA,
Klie
RF,
Morrissey
JH and
Liu
Y
(2016). Artificial dense granules, a potential, biomimetic procoagulant agent composed of inorganic polyphosphate, initiates autoactivation of factor XII.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.02209
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.