Self-assembled colloidal particles with pH sensing property
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1
Zhejiang University, Department of Polymer Science and Engineering, China
Encapsulation of pH sensitive fluorophores as reporting molecules provides a powerful approach to visualize the transportation of colloidal particles. In this study, the dual-labeled microcapsules via nanoparticle/polymer assembly based on polyamine–salt aggregates can be fabricated for the ratiometric intracellular pH sensing. After deposition of SiO2 nanoparticles on the poly(allylamine hydrochloride)/multivalent anionic salt aggregates followed by silicic acid treatment, the generated microcapsules are stable in a wide pH range (3.0~8.0). pH sensitive dye and pH insensitive dye are simultaneously labeled on the capsules, which enable the ratiometric pH sensing. Due to the rough and positively charged surface, the microcapsules can be internalized by several kinds of cells naturally. Real-time measurement of intracellular pH in several living cells shows that the capsules are all located in acidic organelles after being taken up. Furthermore, the negatively charged DNA and dyes can be easily encapsulated into the capsules via charge interaction. The microcapsules with combination of localized pH sensing and drug loading abilities have many advantages, such as following the real-time transportation and processing of the carriers in cells.
Natural Science Foundation of China (21174130)
Keywords:
self-assembly,
biosensing,
Capsule,
Nano/micro particle
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biosensors
Citation:
Tong
W,
Zhang
P and
Gao
C
(2016). Self-assembled colloidal particles with pH sensing property.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01421
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Pan Zhang, Zhejiang University, Department of Polymer Science and Engineering, Hangzhou, China, 75214957@qq.com