3D-Printed FGF-incorporated silk scaffold for skin regeneration
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1
Center for Stem Cells and Tissue Engineering, School of Medicine, China
The key problems hindering skin repair are deficiency of dermal vascularization and difficulty of epidermis regeneration, it is challenging to fabricate scaffolds that can biologically fulfill the requirements for regeneration of skin injuries. To solve this problem, we developed a three-dimensional printed hydrogel-silk fibroin (3DG-SF) matrix scaffold incorporated with basic fibroblast growth factor (FGF-2) through a sulfonic acid group (SO3). The efficacy and mechanism by which the 3DG-SF-SO3-FGF scaffolds promote skin regeneration were systematically investigated both in cell culture and a full-thickness skin defect models. The histological results showed that incorporation of FGF-2 into 3D scaffolds significantly enhanced the regeneration of skin-like tissues as compared to petroleum gauze, after implantation in rat skin defects for 14 and 28 days. Further investigations demonstrated that the 3DG-SF-FGF scaffolds might stimulate the vascularization of dermis. These findings suggest that the incorporation of FGF-2 into 3DG-SF-SO3 scaffolds is a viable strategy for skin regeneration.
Keywords:
Regenerative Medicine,
Bioprinting,
3D scaffold,
tissue niche
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Regenerative medicine: biomaterials for control of tissue induction
Citation:
Zhang
S,
Xiong
S,
Lu
P,
Wu
Y,
Wang
Q,
Zhang
X,
Sun
H and
Ouyang
H
(2016). 3D-Printed FGF-incorporated silk scaffold for skin regeneration.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01297
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.