Introduction: Magnesium, a biodegradable and bioactive metal, has drawn increasing interesting due to its mechanical strength and good biocompatibility. An innovative bioactive porous scaffold composed of poly (lactide-co-glycolide, PLGA), b-tricalcium phosphate (TCP) and magnesium (Mg) with well-defined biomimic microstructure for bone regeneration was designed and fabricated by low-temperature 3D printing technology. In this study, the authors aimed to investigate the in vivo osteogenic and angiogenic effect of PLGA/TCP/Mg porous scaffold. The effect of magnesium in angiogensis and osteogenesis was observed in this study.
Materials and Methods: A steroid–associated osteonecrosis (SAON) rabbit model with 3 mm bone defect tunnel in both distal femora was developed accroding to our published paper[1]. The samples were randomly divided into three groups: (i) control group without any implantation after the surgery, (ii) PLGA/TCP group with PLGA/TCP scaffold implanted into the bone tunnel, and (iii) PLGA/TCP/Magnesium group with PLGA/TCP/Magnesium implanted into the bone tunnel. Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) was used to observe the blood perfusion function at the defect sites at 0,2,4,8 weeks post surgery. Micro-CT scanning and X-ray were used to analysis the in vivo osteogenetic effect of the PLGA/TCP/Magnesium. Bone tissue volume density (BV/TV, %), connectivity density (Conn.D, 1/mm3), trabecular number (Tb.N, 1/mm), in bone tunnel were measured.
Results and Discussion: At week 2 and 4, PLGA/TCP/Mg group performed good blood perfusion and showed significantly higher “maximum enhancement” than the PLGA/TCP group and control group(p<0.05, n=3). Micro-CT data showed that at week 12, the BV/TV, Conn.D and Tb.N of PLGA/TCP/Magnesium group increased significantly than those in control group (p<0.05, n=8).
Conclusions: The in vivo study shows that the PLGA/TCP/Mg scaffolds have good osteogenetic and angiogenic effect and the scaffold is a promising biomaterial for bone regeneration.
The authors are grateful for the financial supports from funding NSFC-DG-RTD Joint Scheme (Project No. 51361130034),the European Union’s 7th Framework Program under grant agreement n° NMP3-SL-2013-604517, NSFC grant (Project No. 51203178), and Shenzhen Fundamental Research Foundation (Project No. JCYJ20120617114912864).
References:
[1] Qin L, Zhang G, et al. Multiple bioimaging modalities in evaluation of an experimental osteonecrosis induced by a combination of lipopolysaccharide and methylprednisolone. Bone, 2006, 39: 863-871.