IMMUNOCHEMICAL ASSAY OF CARCINOGENIC TOXIN IN MILK
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1
Djillali Liabes University, Biology, Algeria
Introduction
The present study was aimed to analyze the presence and levels of Aflatoxin M1 (AFM1), « considered as a proved carcinogen » [1,2], in the raw and the recombined milk, on one hand, and to study the kinetics of its degradation due to the lactic acid bacterias throughout the process of the production of Leben (whey or fermented milk) on the other hand.
Materials and methods
- Immunochemical assay of the rate of AFM1 in the raw and recombined milk intended for the manufacturing of Leben (whey);
- Evaluation of the degradation, of this toxin, by the lactic acid bacteria, by using the the competitive ELISA method.
Results
AFM1 rate determined by competitive ELISA in raw milk and recombined one, gave similar rates, of 119,057. 10-3 µg / kg, which are 2,38 times higher than the maximum recommended by the regulatory.
An evident correlation is revealed between the evolution of the AFM1 rate and the acidity whether it is for Leben with raw milk or that with recombined milk. Indeed after 18 hours of maturation of Leben with raw milk, the AFM1 and acidity rates are, respectively, 32,991. 10-3 µg/kg and 73 °D, with a degradation rate of 88,29 %. Also for Leben with recombined milk, these same rates are, in the respective order, 29,405. 10-3 µg / kg and 78°D, with a degradation rate of 89,36 %.
Conclusion
Aflatoxins are highly toxic, immunosuppressive, mutagenic, teratogenic, and carcinogenic compounds. The main target organ for their toxicity and carcinogenicity is the liver. For this reason, AFM1 in milk and dairy products should be controlled. We finally held that the consumption of Leben, contrary to milk, is more reassuring and present few risks of poisoning due to the AFM1.
References
1. Pfohl-Leszkowicz A. Mycotoxines : facteur de risque de cancers. J. Afr. Cancer. 2009 ; 1 (1): 42-55
2. CIRC.Aflatoxins. IARC Monograph on the Evaluation of Carcinogenic Risks to Humans. WHO. 2002; 82: p 171, IARC, Lyon, France.
Keywords:
Aflatoxin M1,
carcinogenic,
ELISA method,
immunochemical Assay,
Milk.
Conference:
The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014.
Presentation Type:
Poster Presentation
Topic:
Immunity – Nutrition – Cancer
Citation:
DRA
G and
BENALI
M
(2014). IMMUNOCHEMICAL ASSAY OF CARCINOGENIC TOXIN IN MILK.
Front. Immunol.
Conference Abstract:
The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014).
doi: 10.3389/conf.fimmu.2014.04.00020
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Received:
20 Sep 2014;
Published Online:
01 Dec 2014.
*
Correspondence:
Mrs. Ghislaine DRA, Djillali Liabes University, Biology, Sidi Bel Abbes, Sidi Bel Abbes, 22000, Algeria, yaralife@yahoo.fr