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Phylogeography of the species Trachurus picturatus (Bowdich 1825) inferred from mitochondrial and nuclear DNA sequence data

Cláudia Moreira1, 2*, Alberto T. Correia2, 3 and Elsa Froufe2
  • 1 Instituto Ciências Biomédicas Abel Salazar da Universidade do Porto (ICBAS-UP), Portugal
  • 2 Centro Interdiscilinar de Investigação Marinha e Ambiental (CIIMAR-CIMAR), Portugal
  • 3 Faculdade de Ciências da Saúde da Universidade Fernando Pessoa (FCS-UFP), Portugal

The populations of marine pelagic fishes, in particular the migratory ones as the Trachurus picturatus (Bowdich 1825), may be erroneously considered an homogenous population unit because they show broad geographic distributions, large population sizes and high migratory movements. The blue jack mackerel, T. picturatus is widely distributed in the NE Atlantic and also found in the Mediterranean and Black Seas. It is an economically important resource in the Macaronesian islands of Azores, Madeira and Canaries, but despite its fishery value and ecological importance, fluctuations in the landings are difficult to explain since studies regarding the population dynamics, stocks structure, fish movements and habitat connectivity are inexistent. Moreover, genetic methods that can act as a complementary tool for stock identification and population dynamics have never been applied to this species. The blue jack mackerel was sampled by artisanal fleets in the Islands of Azores, Madeira and Canaries, and at the Portuguese mainland - Matosinhos, Peniche and Portimão -during the spring 2016. A tissue sample was taken from each individual and stored in 96% ethanol for molecular analysis. Total DNA was successfully extracted, followed by a Polymerase Chain Reaction amplification of mitochondrial and nuclear DNA. Amplifications and sequencing of the mitochondrial (Cyt B, COI and control region) and nuclear (S7) genes were carried out using published primers used for the Carangidae family (Cárdenas et al. 2005) and for marine fish species (Almada et al. 2016). Sequences from Greece and Turkey, available on GenBank, were added to our sequences and all analysed together. In total 46 sequences were analysed, including the closely related outgroup (Trachurus Trachurus). Preliminary results show that within the samples collected one was proven to be miss identified, being T. Trachurus. Preliminary phylogeographic analyses revealed a very shallow phylogenetic tree and a star-like haplotype network, with haplotypes shared among populations. Further studies, including the number of sequences and the development of microsatellites are been conducted as part of a PhD program.

Acknowledgements

The first author has a grant from the Portuguese Foundation for Science and Technology (SFRH/BD/111133/2015). This article is a result of the project MarInfo – Integrated Platform for Marine Data Acquisition and Analysis (NORTE-01-0145-FEDER-000035) supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).

References

Almada F., Casas L., Francisco, S. M., Villegas-Ríos, D., Saborido-Rey, F., Irigoien, X., Robalo, J. I. (2016). On the absence of genetic differentiation between morphotypes of the ballan wrasse Labrus bergylta (Labridae). Marine Biology, 163:86
Cárdenas L, Hernández C. E., Poulin E, Magoulas A, KornWeld I and Ojeda F. P. (2005). Origin, diversification, and historical biogeography of the genus Trachurus (Perciformes: Carangidae). Molecular Phylogenetics and Evolution 35, 496-507.
GenBank—http://www.ncbi.nlm.nih.gov/genbank/

Keywords: Trachurus picturatus, habitat connectivity, mitochondrial DNA, nuclear DNA, Phylogeography

Conference: XIX Iberian Symposium on Marine Biology Studies, Porto, Portugal, 5 Sep - 9 Sep, 2016.

Presentation Type: Poster Presentation

Topic: 4. FISHERIES, AQUACULTURE AND BIOTECHNOLOGY

Citation: Moreira C, Correia AT and Froufe E (2016). Phylogeography of the species Trachurus picturatus (Bowdich 1825) inferred from mitochondrial and nuclear DNA sequence data. Front. Mar. Sci. Conference Abstract: XIX Iberian Symposium on Marine Biology Studies. doi: 10.3389/conf.FMARS.2016.05.00113

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Received: 30 Apr 2016; Published Online: 03 Sep 2016.

* Correspondence: Miss. Cláudia Moreira, Instituto Ciências Biomédicas Abel Salazar da Universidade do Porto (ICBAS-UP), Porto, Portugal, claudyamoreira@gmail.com