A physiological study of integument secretions in the marine polychaete Eulalia viridis and their potential biotechnological value.
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1
IMAR – Instituto do Mar, Departamento de Ciências e Engenharia do Ambiente, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, Portugal
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2
CESAM-Centro de Estudos do Ambiente e do Mar, CiiEM-Centro de Investigação Interdisciplinar Egas Moniz, Portugal
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3
Centro de Biologia Ambiental, Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa, Portugal
There is a growing interest in marine bioproducts, especially in those with life improving potential to humans. Marine invertebrates, in particular, possess particular interest due to their high diversity, abundance and ecological importance. However, the physiology of such a wide and diverse group of organisms is far from understood. Marine annelids, in particular (Class Polychaeta), are of especial interest, in large part owing to their ecological representativity and easy handling. Still only few studies have been focusing on physiological traits of the polychaeta that may relate to the production of bioproducts of potential biotechnological applications. The secretion of substances by polychaetes is acknowledged to relate primarily with locomotion and feeding, but also thought to have an important role in egg protection, prevention against infection and even as defence mechanisms against possible predators. As such, the present work aims at identifying, isolating and analysing the nature of integumentary secretions of E. viridis, a common intertidal worm of rocky shores. Histological analyses revealed a more complex microanatomy of the skin than it could be anticipated. Several cell types were identified in the integument, each bearing different functions such as structural support, pigmentation and sensorial, to which is added secretion as one of the most important roles. Due to the great complexity of the integument, the identification of the chemical nature of all types of secretions is particularly challenging. However, more detailed histochemical analyses and electron microscopy enabled the identification of different secretory cells and their chemical nature and role. The analyses was complemented by protein isolation from mucous and homogenates trough SDS-PAGE. Extracts revealed, for instance, that peptidic substances produced by the skin are potentially biocidal, as assessed through the Microtox Assay. Overall, the results enhance the importance of studies with marine invertebrates, especially polychaetes, for the isolation of natural biocides, antioxidants and other substances of high biotechnological value. However, further research is still needed to fully isolate and identify such substances and the physiological processes underlying their production in marine invertebrates in general.
Acknowledgements
The authors acknowledge Fundação para a Ciência e Tecnologia for the grant SFRH/BPD/72564/2010 to P.M. Costa and for funding the project EXPL/MAR–EST/1236/2012, including the fellowship to A. Rodrigo. The authors also acknowledge the contributions of C. Gonçalves, C. Martins and J. Santos (IMAR) and P. Henriques (CiiEM).
Keywords:
Annelida,
Biocidals,
Natural Products,
Goblet Cells,
marine biotechnology
Conference:
IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014.
Presentation Type:
Oral Presentation
Topic:
BLUE BIOTECH
Citation:
Rodrigo
AP,
Alves De Matos
AP,
Carrapiço
F,
Costa
MH and
Costa
PM
(2014). A physiological study of integument secretions in the marine polychaete Eulalia viridis and their potential biotechnological value..
Front. Mar. Sci.
Conference Abstract:
IMMR | International Meeting on Marine Research 2014.
doi: 10.3389/conf.fmars.2014.02.00040
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Received:
16 May 2014;
Published Online:
18 Jul 2014.
*
Correspondence:
Miss. Ana P Rodrigo, IMAR – Instituto do Mar, Departamento de Ciências e Engenharia do Ambiente, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, Caparica, Portugal, a.rodrigo@campus.fct.unl.pt