Comparison between colloidally-confined and molecularly-dissolved polyphosphates for activating the contact pathway of blood coagulation
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1
University of Illinois at Chicago, Department of Chemical Engineering, United States
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2
University of Illinois at Urbana-Champaign, Department of Biochemistry, United States
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3
University of Illinois at Chicago, Department of Biopharmaceutical Sciences, United States
Polyphosphate-functionalized gold nanoparticles (polyP-GNPs) were synthesized by phosphoramidate conjugation chemistry. More specifically, platelet-sized polyphosphate was first conjugated with cystamine via EDAC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride)-mediated reaction and attached to gold nanoparticles (GNPs) of various sizes via reduction of the disulfide moiety in cystamine. The procoagulant activity of the synthesized polyP-GNPs was quantified by assaying for the contact pathway of clotting in human pooled normal plasma (PNP). It was demonstrated that the particles are excellent contact pathway activators, significantly better than molecularly dissolved, platelet-sized polyP of the same chain length. Furthermore, their activity was found to be equivalent to that of long chain polyP (more than ten times longer than platelet-sized polyP) commonly found in bacteria. These results strongly support the hypothesis that localization of procoagulant agents such as polyP on colloidal surfaces markedly improves their pharmacological efficacy. These newly synthesized polyP-GNPs may serve as promising hemostatic agents to treat both short- and long-term bleeding events.
Keywords:
nanoparticle,
surface property,
biomacromolecule,
Biofunction
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Nano-structured materials for unique functions
Citation:
Szymusiak
M,
Donovan
AJ,
Smith
SA,
Ransom
R,
Shen
H,
Kalkowski
J,
Morrissey
JH and
Liu
Y
(2016). Comparison between colloidally-confined and molecularly-dissolved polyphosphates for activating the contact pathway of blood coagulation.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.02179
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.